160 results about "Trametes versicolor" patented technology

A composite biological agent with ternary microorganism for composting process of agricultural waste is composed of a composite biological agent I, a composite biological agent II, and a composite biological agent III, which is characterized in that the composite biological agent I is composed of bacillus, actinomycetes, microzyme and lactobacillus, the composite biological agent II is composed of trichoderma, ergillus, penicillium and trametes versicolor, and the composite biological agent III is composed of photosynthetic bacterium, azotobacter, phosphorus bacteria, and silicate bacterium. 30-60% composite biological agent I, 30-60% composite biological agent II, 10-20% composite biological agent III are mixed or applied to the process of composting process of agricultural waste in phases. Each component ratio and recruitment of biological agents can be both properly adjusted with different dung materials and dung purpose, thereby achieving purpose of using microorganism resources efficiently and raising fermentation efficiency.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention provides a traditional Chinese medicine composition for treating complication of uremia caused by hematodialysis, which comprises astragalus, dangshen, Chinese yam, semen coicis, carthamus tinctorius, frangula emodin, herba orostachyos, dendrobe, arillus longan, dandelion, angelica sinensis, fructus kochiae, kelp, motherwort, radix salviae miltiorrhizae, pokeberry root, morinda officinalis, trametes versicolor, fructus psoraleae and acanthopanax root. The traditional Chinese medicine composition can well treat the complication such as skin itch and malnutrition caused by hematodialysis, and has the advantages of being high in treatment effect, short in course of treatment and rapid in taking effect when being used for treating the uremia by being matched with hematodialysis.

The invention belongs to the field of monosaccharide component analysis, and in particular relates to a method for detecting monosaccharide components in rainbow conk glycopeptide. The method comprises the following steps of: carrying out a test on HPLC (High Performance Liquid Chromatography) chromatographic condition and systematic compatibility; subsequently respectively absorbing a mixed reference solution and a test solution to be injected into the HPLC chromatographic instrument so as to obtain a chromatogram, wherein the reference solution is a 1phenyl-3-methyl-5-pyrazolone derivative solution of D-glucose, D-mannose, D-xylose, L-xylose, D-galactose, D-arabinose, D-hydrochloric acid glucosamine and L-rhamnose reference solutions; and the test solution is a 1-phenyl-3-methyl-5-pyrazolone derivative solution of a rainbow conk glycopeptide acid hydrolysis product. Polysaccharide peptide products such as rainbow conk glycopeptide, rainbow conk intracellular glycopeptide, rainbow conk extracellular glycopeptides and coriolus versicolor polysaccharide are distinguished by detecting the mole ratio of eight monosaccharide ingredients in a test sample, so that the quality and the curative effect of the rainbow conk glycopeptide are ensured.

The invention discloses a pig biological feed additive capable of effectively enhancing immunity and belongs to the field of feed additives. The additive comprises a microecological preparation, a compound Chinese herbal medicine extract, rhizoma drynariae powder, platycodon grandiflorum powder, lotus seed powder, caulis polygoni multiflori powder, trametes versicolor powder, mung bean powder, arginine, vitamin C, vitamin E, ferrous citrate and a carrier, wherein the carrier comprises defatted rice bran and bentonite. The additive can effectively enhance the immunity of pigs, reduce the morbidity and mortality of the pigs, improve the health degree of the pigs and replace antibiotics to be applied in the production of live pigs, and further has very broad application prospects.

The invention relates to a pretreatment fungicide for xylose residue or furfural residue, a preparation method and an application thereof, belonging to the technical field of agricultural microbiology. The invention relates to a microbial composite fungicide specially used for promoting the xylose residue or the furfural residue to degrade and improving the production efficiency of methane. The invention firstly adopts liquid fermentation technology to purely breed and ferment all components of the microbial fungicide, and then the components are compounded. The preparation method of the pretreatment fungicide comprises: 0.20-0.40 parts by weight of trichoderma viride liquid, 0.10-0.15 parts by weight of phanerochaete chrysosporium liquid, 0.05-0.10 parts by weight of aspergillus niger liquid, 0.08-0.16 parts by weight of aspergillus oryzae liquid and 0.05-0.10 parts by weight of trametes versicolor liquid, and the dry weight of fermentation hyphae is more than 20g / L. The composite fungicide can be used for improving the composite fungicide speed of the xylose residue or the furfural residue as well as the methane production efficiency; the methane production efficiency is improved by 30-60%; the fermentation time is shortened; the start period is shortened by 3-10 days; and the invention is also good in the effects on the aspects of pretreatment of smashed straws, and methane production.

The invention relates to Trametes Versicolor YS-L613 and a breeding method and application thereof. The Trametes Versicolor YS-L613 is prepared by the method through mutation breeding. The breeding method is to take Trametes Versicolor as a starting strain, adopt composite mutation breeding technology which combines UV, 60 CO, ultraviolet rays, nitrosoguanidine, ion implantation and microwave treatment, and breed the YS-L613 strain for high-yield production of laccase. The invention performs breeding operation through physiochemical treatment methods such as ion implantation, the ultraviolet rays and mutagen, finally obtains the YS-L613 strain for high-yield production of the laccase, and adopts experimental automatic control technology, strain fermentation control technology and strain extraction technology to develop laccase products. As proved by experiments of the laccase in pulp bleaching, fiber modification and pulp deinking, utilization of the laccase in the paper-making industry can improve the quality of paper, reduce environmental pollution and save the cost.

The invention discloses a method for extracting trametes versicolor exopolysaccharide through a two-membrane process, which comprises the steps that trametes versicolor fermentation liquor is sequentially subjected to microfiltration and ultrafiltration for separation to obtain trametes versicolor exopolysaccharide. According to the method, the conventional 'water extraction and alcohol precipitation' extraction mode of trametes versicolor exopolysaccharide is changed, the extraction effect of the active ingredients of the trametes versicolor exopolysaccharide is improved, the ethanol usage during the production is lowered, the filtrate wastewater discharge is reduced, and the economic and social benefits are obviously increased.

This invention belongs to the field of drug-used microbe fermentation, specifically it relates to stictin endocellular glicotide yielding bacterium and its fermentation plating method. The originator screen and get stictin endocellular glicotide yielding bacterium: stictin (Coriolus versicolor)CCTCC M204081, which can give birth to high-strength effective composition; he also supplies a new method for optimizing fermentation process by supplementing and controlling pH value. This invention also discloses a new extraction technique about how to extract stictin endocellular glicotide from mycelium. This method can enhance the quality of mycelium and this is good to synthesize stictin endocellular glicotide, therefore the productivity is advance.

The invention discloses a preparing method of oxalic acid decarboxylase with fungi, which comprises the following steps: (1) moist-heat-sterilizing organic acid or inorganic acid solution with high temperature at 121 deg. c; (2) setting the initial pH value of liquid medium of trametes versicolor (Trametes versicolor) or aspergillus niger (Aspergillus niger) and so on fungi at 5. 0-5. 5; setting the reacting temperature at 20-25 deg. c and air capacity at 20-30L / L . h; adding the acid solution at the third or fourth day with pH value at 0-3; centrifuging; collecting thallus cell; or filtering; getting thallus cell; eluting with o. 05-0. 2M and pH value at 3-3. 7 acetic acid buffer solution; collecting; freezing; grinding hypha; adding into acetic acid-sodium acetate buffer solution with 0. 05-0. 2M and pH value at 3-3. 7; grinding to pasty continually; centrifuging; getting oxalic acid decarboxylase liquid. This invention possesses low cost, which can be used to medical, food, beverage, agriculture and so on aspects.

The invention discloses a method for improving Trametes versicolor laccase output. The method includes: at the mycelium growth stage of a Trametes versicolor laccase fermentation process, adding farnesol into a fermentation liquor, and further performing fermentation to produce laccase. By applying the method, the laccase output of the Trametes versicolor can be greatly improved. Also, the method is simple in operation, is easy for enlarged production, and is suitable for large-scale laccase fermentation, thereby reducing the laccase production cost, and meeting the requirements of large-scale application.

The invention discloses a prescription for treatment of urticaria, and the prescription comprises the following substances: by weight, 10-30 parts of radix astragali, 5-20 parts of poria cocos, 10-30 parts of red bean, 5-15 parts of adenophora root, 5-15 parts of safflower, 10-20 parts of lithospermum, 10-25 parts of dahurian angelica root, 10-30 parts of safflower, 10-20 parts of radix scrophulariae, 10-20 parts of lotus leaf, 20-30 parts of sophora flower, 10-20 parts of balloonflower root, 10-20 parts of senecio, 10-20 parts of Chinese angelica, 15-20 parts of Chinese wax gourd Peel, 5-10 parts of rainbow conk, 5-25 parts of root of kudzu vine, 5-20 parts of pawpaw, 10-30 parts of bighead atractylodes rhizome, 5-20 parts of periostracum cicadae, 5-20 parts of lumbricus, 5-20 parts of magnolia officinalis and 5-20 parts of schizonepeta.

The invention relates to a preparation method for Coriolus versicolor glucan. The preparation method for the Coriolus versicolor glucan is characterized by comprising the following steps of: extracting Coriolus versicolor HT-6 (collection registration number: CGMCC (China General Microbiological Culture Collection Center) No.6760) mycelia through Na2CO3 and an enzyme-ultrasonic wave combined method; then, performing filtration and vacuum concentration on extracting solution; removing protein by using a Sevag method; performing decoloration treatment by using activated carbon; collecting supernate in the manner of centrifugal separation; performing sediment by using 95 percent of absolute ethyl alcohol; performing filter washing on precipitates by using absolute ethyl alcohol; and finally performing vacuum drying to obtain the Coriolus versicolor glucan. According to the preparation method disclosed by the invention, the extraction efficiency of the Coriolus versicolor glucan can be remarkably improved, and moreover, the operation time is obviously shortened, and the energy consumption is reduced. The preparation method is environmentally friendly; an adopted instrument is high in degree of automation and simple in operation, and therefore, the industrial production of the Coriolus versicolor glucan becomes possible.

The invention discloses a banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and a preparation method thereof. The banana stem and leaf residue decomposing inoculant is prepared by using trametes versicolor with a cellulose, lignin and hemicellulose decomposing function, trichoderma citrinoviride, aspergillus oryzae, bacillus amyloliquefaciens with a biological control function, and streptomyces pristinaespiralis for compound fermentation. The banana stem and leaf residue decomposing inoculant can effectively accelerate the decaying of banana stem and leaf residues returned to the field, while inhibiting the growth of fusarium wilt of banana and other pathogenic bacteria; the decomposition of the banana stem and leaf residues is accelerated after applying the decomposing inoculant in the field, wherein the weight loss rate of the banana stem and leaf residues is increased remarkably; the tensile force of the banana stem and leaf residues is reduced remarkably; the number of soil pathogens including antagonistic bacillus and actinomycetes is increased remarkably; and the number of pathogenic organisms is reduced remarkably.

The invention relates to an extraction method of polysaccharides in coriolus versicolor. The method comprises the steps as follows: freezing and drying a proper amount of coriolus versicolor to the constant weight, and crushing and sieving the coriolus versicolor; placing the sieved sample powder into a slant culture medium for culture; placing activated strains into a fermentation flask for shaking flask fermentation culture to obtain a strain fermentation liquid; adding the same amount of distilled water into the strain fermentation liquid for dissolution and dilution, performing multiple times of microwave extraction, performing centrifuging, taking supernatant for rotary evaporation, adding absolute ethyl alcohol, performing ultrasonic even mixing, placing a mixture in a refrigerator of 4 DEG C overnight, performing centrifuging, discarding the supernatant, drying sediment to the constant weight, and obtaining the polysaccharides in the coriolus versicolor. According to the microwave extraction method adopted in the extraction method, extraction conditions are strictly optimized, the extraction time is in a range of 20-30 s, the extraction power is in a range of 260-265 W, the extraction temperature is in a range of 55-60 DEG C, and the number of extraction times is in a range of 3-5, so that the polysaccharides in the coriolus versicolor can be fully extracted, the extraction ratio is highest, meanwhile, the polysaccharides in the coriolus versicolor are prevented from being damaged, and compared with traditional methods, the extraction method is simpler to operate and short in operation time.

The invention relates to a coriolus and lucid ganoderma edible mushroom and a preparation method thereof. The preparation method comprises the following steps of preparing first-grade mother spawns; preparing second-grade stock spawns from the obtained first-grade mother spawns; preparing third-grade cultivated spawns from the obtained second-grade stock spawns; sowing, fruiting, harvesting and drying the obtained third-grade cultivated spawns to obtain the coriolus and lucid ganoderma edible mushroom, wherein the first-grade mother spawns are prepared by adopting a selenium-enriched potato agar culture medium; every 1000mL of selenium-enriched potato agar culture medium is prepared from the following raw materials: 160 to 240g of potato, 16 to 23g of saccharides, 1 to 8g of magnesium sulfate, 1 to 5g of monopotassium phosphate, 15 to 30g of peptone, 0.5 to 5g of VB1, 0.1 to 3g of sodium selenite, 1 to 8g of calcium carbonate, 1 to 8g of ferrous sulfate, 1 to 8g of zinc sulfate and 15 to 30g of agar. The coriolus and lucid ganoderma edible mushroom prepared by the preparation method disclosed by the invention is high in fruiting rate which can reach 80 percent or above; in addition, the coriolus and lucid ganoderma edible mushroom is thick in mushroom and is good in mouthfeel; besides, by domestication, the contents of selenium and zinc in the coriolus and lucid ganoderma edible mushroom are improved.

The invention provides a traditional Chinese medicine composition for treating complication of uremia caused by hematodialysis, which comprises astragalus, dangshen, Chinese yam, semen coicis, carthamus tinctorius, frangula emodin, herba orostachyos, dendrobe, arillus longan, dandelion, angelica sinensis, fructus kochiae, kelp, motherwort, radix salviae miltiorrhizae, pokeberry root, morinda officinalis, trametes versicolor, fructus psoraleae and acanthopanax root. The traditional Chinese medicine composition can well treat the complication such as skin itch and malnutrition caused by hematodialysis, and has the advantages of being high in treatment effect, short in course of treatment and rapid in taking effect when being used for treating the uremia by being matched with hematodialysis.

The invention discloses a detection method of polystictus versicolor and a preparation containing polystictus versicolor. According to the method, monosaccharide fragments obtained after polysaccharides of polystictus versicolor and the preparation containing polystictus versicolor are hydrolyzed are detected by adopting ultra performance liquid chromatography or high performance liquid chromatography while mannitose, glucose, galactose, fucose and other monosaccharide fragments are taken as evaluating indicators, and a quality control and evaluation method for measuring characteristic spectra and content of the monosaccharide fragments is established. The method can be used for quality control and evaluation of a polystictus versicolor crude drug and a preparation containing the polystictus versicolor crude drug, a polystictus versicolor extract or a polystictus versicolor effective part. The method is used for evaluating the quality of the polystictus versicolor crude drug and the preparation by controlling the monosaccharide fragments obtained after polysaccharide of the polystictus versicolor and the preparation containing polystictus versicolor are hydrolyzed. The method is strong in specificity, high in accuracy, easy and simple to operate, easy to control and higher in practical value, and can be used for effectively controlling the product quality.

The invention relates to a preparation method of a physiological function regulator solution rich in triterpenoid glycopeptide, in particular to a preparation method of preparing the physiological function regulator solution. The preparation method of a physiological function regulator rich in triterpenoid glycopeptide adopts the technical scheme comprising the four steps: preparing rainbow conk zymotic fluid, preparing rainbow conk submerged fermentation liquid filtrate removing mycelium, preparing rainbow conk live bacterial zymotic fluid and adding a protective agent, wherein the preparation of the rainbow conk zymotic fluid comprises the following steps: preparing inclined culture rainbow conk strains, preparing shake flask culture rainbow conk strains, preparing first-order seed culture rainbow conk strains, preparing second-order seed culture rainbow conk strains and preparing rainbow conk submerged fermentation liquid. The invention is applied to the preparation of the physiological function regulator solution rich in the triterpenoid rainbow conk glycopeptides. The invention has the advantages that the physiological function regulator has various biological activities, can remove vegetable protein anti-nutrient factors, enhances the immunity and the disease-resistant capability, stimulates the growth and can be used for nourishing and caring human bodies or be used as animal fodder.

The invention relates to a natural drink, namely compound pin leaf and ganoderma tea, and a making method thereof. The compound pin leaf and ganoderma tea is mainly prepared by mixing 15 percent of pin leaf, 40 percent of ganoderma applanatum, 10 percent of fuscoporia punctata, 5 percent of phellinus pini, 10 percent of phaeoporus obliquus, 10 percent of trametes versicolor and 10 percent of Daxin bitter butyl tea. The making method comprises the following steps of: primarily processing raw materials; weighing and grinding; sterilizing by using steam, and drying in the sun; mixing and quantitatively bagging; performing vacuum packaging; and sterilizing by using steam and drying in the sun to obtain the finished product. During use, a vacuum bag is opened, a filter paper tea bag is put into a pot, boiling water is poured into the pot, and the tea can be drunk. If the tea is frequently drunk, the tea can strengthen the immunity of a human body, improve the disease resistance and diseasecuring capacity of the human body, promote metabolism, and ensures that a user is healthy and the life of the user is prolonged.

The invention discloses a new strain, namely Trametes versicolor T-4 capable of efficiently preprocessing lignin raw materials under the alkaline condition. The Trametes versicolor T-4 is preserved in the CGMCC (China General Microbiological Culture Collection Center) at Sep.2nd in 2014 with the preservation number of CGMCC No.9582, and the address of the CGMCC is Microbiology Research Institute of China Science Academy, No.3 of No.1 yard at Beichen west road of Chaoyang District in Beijing. The Trametes versicolor T-4, provided by the invention, can solve a series of problems that the biological preprocessing of the lignin raw materials is low in efficiency and long in cycle and the like, and has great potential in the aspects such as preparation of fuel ethanol and methane through the utilization of the lignin raw materials, and lays the foundation for the clean, efficient and low-cost preprocessing of the lignin raw materials.

The invention discloses a health tea suitable for use in all seasons, which comprises the following ingredients in parts by weight: 50-100 parts of oolong tea, 10-20 parts of Trametes versicolor, 10-20 parts of Cordyceps sinensis, 10-20 parts of Phellinus tgiarius, 10-20 parts of mulberry leaf, 10-20 parts of Schisandra chinesis, 10-20 parts of Panax quinquefolium, 10-20 parts of Lycium chinense, 10-20 parts of Syringa reticulata, 10-20 parts of ginseng leaf, 10-20 parts of Albizia flower and 5-10 parts of Adonis amurensis flower. The health tea suitable for use in all seasons has the effects of relieving fatigue, nourishing qi and blood, improving immunity, nourishing the heart, tranquilizing the mind and protecting the liver, can be taken for a long time; is convenient to serve and free of toxic or side effect, and can protect the internal organs of a human being.

The invention belongs to the technical field of soil bioremediation, particularly relates to a method for remedying soil of a coal mining area, and discloses a method for achieving plant-microorganismcombined remediation on soil of a coal mining area. According to the method, trisetum bifidum seeds are exposed to the sun, soaked and sprouted, then the sprouted trisetum bifidum seeds are sown in aplug containing a seedling grow matrix for emerging and seedling grow, then a mixed bacterium agent formed by acidithiobacillus ferrooxins, trametes versicolor and glomus coronatum is applied, afterthe polluted soil of the mining area is leveled, improved and ditched, seedlings are buried in ditches for soil return seedling covering, field management is carried out, and after growing for 90-120days, the seedlings are harvested. Through the method, after one or more times of planting, the physicochemical indexes of the soil of the coal mining area can be obviously improved to reach the normal standard, a new way is provided for soil control of wide coal mining areas, and wide application prospects are achieved.

The invention provides a preparing method of a compound microorganism agent for papermaking wastewater. The compound microorganism agent is prepared by mixing Trametes versicolor, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus, Pseudomonas alcaligenes and cellulase; the cellulase can decompose celluloses in the wastewater into glucose; compound microorganism can also degrade solids, lignin and the like in the wastewater. The compound microorganism agent is free of chemicals, environment friendly, non-toxic and free of secondary pollution, is simple to use, is insusceptible to environmental factors, has stable effect on wastewater treatment and allows the quality of effluent to meet the national standard.

The invention relates to a medicine for treating liver cancer, and in particular relates to a traditional Chinese medicine composition for treating liver cancer, which is prepared by taking plant Chinese herbal medicines as raw materials. The traditional Chinese medicine composition is characterized in that effective components are prepared from active pharmaceutical ingredients according to in parts by weight: 10-20 parts of radix pseudostellariae, 10-20 parts of Rhizoma Atractylodis Macrocephalae, 5-10 parts of licorice, 10-20 parts of poria cocos, 10-20 parts of rhizoma alismatis, 10-20 parts of astragalus membranaceus, 20-30 parts of spreading hedyotis herb, 10-20 parts of turtle shell, 20-30 parts of Sculellaria barbata, 15-20 parts of salvia miltiorrhiza, 5-10 parts of centipede, 10-20 parts of hericium erinaceus, 10-20 parts of cortex phellodendri, 10-20 parts of Trametes versicolor, 20-30 parts of fructus schisandrae, 15-20 parts of rhizoma zedoariae, 20-30 parts of fructus aurantii, 15-20 parts of rhizoma sparganii, 15-20 parts of herba artemisiae, 15-20 parts of radix curcumae and 15-20 parts of Gardenia jasminoides Ellis. After the medicines are combined, the traditional Chinese medicine composition provided by the invention has the efficacies of clearing away heat and toxic materials, activating blood circulation to dissipate blood stasis, removing blood stasis, relieving pain, cooling blood, diminishing swelling, calming the liver, purging fire, being antipyretic, dispelling dampness, and the like; due to the synergistic effect of the efficacies, when the medicine is used for treating the liver cancer, the immune function of a body is improved and protected, and the metastasis and diffusion of cancer cells are obviously inhibited; the traditional Chinese medicine composition is few in side effects, low in price, and easy to operate.

The invention relates to a trametes versicolor glucan water agent and a preparation method thereof, in particular to a trametes versicolor glucan water agent for potato Y virus. The water agent comprises the following components in percentage by weight: 1.0 to 2.0 percent of trametes versicolor glucan crude product, 5.0 to 8.0 percent of surfactant alkyl glucoside, 3.0 to 5.0 percent of decomposition-preventing agent 1,2-butanediol, 4.0 to 10.0 percent of antifreezing agent glycerol and the balance of double distilled water. The trametes versicolor glucan crude product is prepared by fermenting the trametes versicolor strain with the collection number of CGMCC No.6760 and extracting the glucan. The average control effect of 500-fold solution of the trametes versicolor glucan water agent on the potato Y virus is more than 78 percent, in particular, the average control effect of 1.1 percent trametes versicolor glucan water agent on the potato Y virus is up to 80.4 percent and is more excellent than 30-fold solution of 20 percent gram Y Tring wettable powder.

The invention relates to the field of traditional Chinese medicines and healthcare products, and especially relates to a composite Ganoderma lucidum extract product for enhancing immunity and inhibiting tumors. The composite Ganoderma lucidum extract product is an oral preparation prepared from Ganoderma lucidum, Ganoderma sinensis, Ganoderma lucidum Karst, Microporus affinis, fruiting bodies of Tyromyces sulpbureus and Trametes versicolor through a modern technology, and preferably a capsule, a tablet and a granule. Pharmacodynamic test confirms that the composite Ganoderma lucidum extract product has remarkable immunity enhancing and tumor inhibiting effects; the product can alleviate cancer pains and obviously improve the living quality of cancer patients; and the production process of the product is simple, and all the raw materials are natural fungi, so the product is safe to take, has a moderate price, and can be easily received by general consumers.

The invention relates to a method for producing a trametes versicolor immunomodulatory protein by virtue of a pichia pastoris expression system. Problems about the preparation of trametes versicolor immunomodulatory proteins are effectively solved. The method comprises the following steps of synthesizing a nucleotide sequence SEQ ID NO.1 of a trametes versicolor immunomodulatory protein FIP-tvc according to a codon usage bias of pichia pastoris; constructing a recombinant expression plasmid X-FIP-tvc by virtue of the trametes versicolor immunomodulatory protein FIP-tvc synthesized by restriction enzyme digestion and an expression plasmid vector X, linearizing the recombinant expression plasmid X-FIP-tvc by virtue of a restriction enzyme, then transforming a competent cell of pichia pastoris GS115, screening a constitutive positive transformant or inducible positive transformant by virtue of an antibiotic-containing plate and PCR (Polymerase Chain Reaction) method, selecting an inducible high-level rFIP-tvc secretion expression pichia pastoris recombinant strain, performing fermental cultivation or induction expression for 3 to 7 days, precipitating fermentation supernatant with ammonium sulfate, performing dialysis on precipitates for 24 to 48 hours, performing affinity exchange chromatography with a chromatographic column, and performing drying to obtain solid powder of the trametes versicolor immunomodulatory protein FIP-tvc. The method is low in cost, short in cycle, easy to implement technically, suitable for industrial production and high in purity.

The invention discloses a straw biodegradation complex bacterial agent. The complex bacterial agent is a compound of bacillus subtilis, bacillus amyloliquefaciens, bacillus cereus, phanerochaete chrysosporium and trametes versicolor. The invention belongs to the environmental-protection technology, a chemical pulping method commonly used in the current pulping technology is replaced by microbial degradation of plant straw, thus the environmental pollution caused by the use of a large number of chemical reagents in the traditional pulping process is avoided, in the production process of pulping, water can be recycled without discharging any sewage containing organic compounds and suspended solids, the link of sewage treatment is avoided, the production cost is saved, in the whole productionprocess, heating is not needed, decomposing is conducted at normal temperature, and bleaching is conducted at normal temperature. Meanwhile, the invention further discloses a preparation method and application of the straw biodegradation complex bacterial agent.

The invention discloses a liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and a breeding method thereof. The preservation number of the bacterial strain is CGMCC No. 10903; the classification name of the bacterial strain is coriolus versicolor in Latin. The polysaccharide yield of the coriolus versicolor mycelium mutated through the method is higher than that of a coriolus versicolor mutant strain of an original strain, the yield of coriolus versicolor polysaccharide is improved, and high economic benefits can be created.