19 results about "Trametes versicolor" patented technology

The present application relates to the technical field of microbial fermentation, in particular to a polyporus arctorus and a culture method and application thereof. A polyporus arctorus YH-DB-1 is screened in the present application, and a large amount of glycopeptide can be extracted from the mycelium of the strain by culturing the strain, the peptide content in the glycopeptide can reach more than 45%, and the present application solves the problem of low peptide yield in the existing polyporus arctorus glycopeptide.

The invention provides a method for efficiently extracting ursolic acid from loquat leaves and application of the ursolic acid. Comprising the following steps: S1, taking fresh folium eriobotryae, cleaning, and drying at 45 DEG C until the water content is 1t; after 8%, crushing, sieving with a 60-mesh sieve, and sterilizing with microwaves to obtain sterilized loquat leaf powder; s2, mixing sterilized folium eriobotryae powder, a yeast extract, MgSO4. 7H2O and water, adjusting the pH value to 5.0, taking the mixture as a fermentation culture medium, synchronously inoculating a 5% saccharomyces cerevisiae seed solution and a 10% trametes versicolor seed solution, and performing shake culture at the temperature of 30 DEG C and the speed of 150 rpm for 36-48 hours; s3, adding a surfactant, uniformly stirring, and performing ultrasonic extraction to obtain an extracting solution; and S4, centrifuging the extracting solution at 4000-5000 rpm for 10-15 minutes, taking supernate, filtering with a filtering membrane, concentrating and freeze-drying. According to the method, double-bacterium synchronous synergistic fermentation and a high-dose surfactant-assisted ultrasonic extraction technology are combined, so that a strong synergistic effect is formed, and the problems of low extraction rate, poor purity, high energy consumption, large solvent dosage and the like in a traditional loquat leaf ursolic acid extraction method are fundamentally solved.

A mutant of laccase from *Trametes versicolor* with enhanced redox potential, along with its screening and preparation method, belongs to the field of biocatalysis technology. To address the technical problem that the low redox potential of laccase, as an oxidase, limits its broad catalytic spectrum, this invention analyzes the relationship between axial coordination residues and redox potential in *Trametes versicolor*-derived laccase to determine mutation sites. Furthermore, by predicting the distance between the active center Cu1 and the axial coordination residues of different mutants, mutants suitable for further modification are screened. Finally, by measuring the redox potential and ABTS activity of different mutants, a mutant with the amino acid sequence shown in SEQ ID NO.3 is obtained. Compared to wild-type *Trametes versicolor*-derived laccase, this mutant exhibits significantly enhanced redox potential and ABTS activity, demonstrating high application potential.

This invention provides a highly efficient extraction method for ursolic acid from loquat leaves and its application. The method includes the following steps: S1. Fresh loquat leaves are taken, washed clean, dried at 45℃ until the moisture content is <8%, pulverized, passed through a 60-mesh sieve, and microwave-sterilized to obtain sterilized loquat leaf powder; S2. The sterilized loquat leaf powder, yeast extract, MgSO4·7H2O, and water are mixed, and the pH is adjusted to 5.0 as a fermentation medium. Simultaneously, 5% *Saccharomyces cerevisiae* seed culture and 10% *Trametes versicolor* seed culture are inoculated and cultured at 30℃ and 150 rpm for 36-48 h with shaking; S3. A surfactant is added, stirred evenly, and ultrasonically extracted to obtain the extract; S4. The extract is centrifuged at 4000-5000 rpm for 10-15 min, the supernatant is filtered through a membrane, concentrated, and freeze-dried. This invention combines simultaneous synergistic fermentation by two bacteria with high-dose surfactant-assisted ultrasonic extraction technology, resulting in a powerful synergistic effect. This fundamentally solves the problems of low extraction rate, poor purity, high energy consumption, and large solvent consumption in traditional loquat leaf ursolic acid extraction methods.

This invention relates to the field of edible fungus preservation research, specifically to a composite preservation film containing ethyl maltol and its preparation method. The purpose of this invention is to produce a traditional Chinese medicine composite preservation film containing ethyl maltol with CMC-Na as a carrier. This composite preservation film, due to the presence of ethyl maltol, which significantly inhibits mold growth, combined with the antibacterial effect of *Trametes versicolor* polysaccharide, provides significant mold prevention while preserving freshness. The traditional Chinese medicine composite preservation film obtained by this invention is uniform, aesthetically pleasing, and breathable, with a thickness of only 0.1-0.2 mm. Using it to preserve *Pleurotus ostreatus* at room temperature extends the preservation time to more than 9 days. It is simple and convenient, with no direct contact between chemical reagents or other liquids and the surface of the edible fungus, and it can be reused.

Described herein are combined fungal compositions for treating, prophylaxis of, or ameliorating symptoms of one or more adverse reactions triggered by an infectious disease or condition that causes an inflammatory response. In one aspect the composition comprises an aqueous or solid fraction of Trametes versicolor and Fomitopsis officinalis mycelium, fermented substrates thereof, or combinations thereof.

This invention belongs to the field of daily chemical products and discloses a polysaccharide composition that improves skin immunity and strengthens the skin barrier function, as well as its applications. The polysaccharide composition includes Scutellaria baicalensis polysaccharide, Adenophora stricta polysaccharide, and Trametes versicolor polysaccharide. Through the synergistic effect of these three polysaccharides, it can not only reduce the number of mast cells but also increase antimicrobial peptides and filaggrin; increase filaggrin; and decrease monocyte chemotactic protein-1 and interleukin-6, thereby achieving the effects of improving skin immunity, anti-inflammatory and whitening. Furthermore, it can act as a signaling molecule, widely participating in physiological processes such as intercellular ligand-receptor recognition and intercellular signal transduction, reducing the average relative amplification fold of interleukin-6 expression factor mRNA, and effectively participating in epidermal cell differentiation and skin barrier formation, thus strengthening the skin barrier function; it can be used in the preparation of cosmetics.

The invention relates to a microbial fermentation technology, and discloses coriolus versicolor, a microbial agent containing the coriolus versicolor, and a culture method and application of the coriolus versicolor. The preservation number of the trametes versicolor is CCTCC (China Center for Type Culture Collection) NO: M2025762, and the preservation number of the trametes versicolor is CCTCC NO: M2025762. The invention provides application of the coriolus versicolor in fermentation production of active ingredients and / or preparation of functional products, and the active ingredients comprise flavonoid compounds and / or terpenoids. The coriolus versicolor is stable in fermentation performance, low in cost, high in biomass and hypha content and rich in various flavonoid and terpene active ingredients.

This invention discloses a specific primer pair, method, and application for detecting *Trametes versicolor*. The specific primer pair is used to amplify gene fragments of *Trametes versicolor*, including primer pair 1 and / or primer pair 2, with the following nucleotide sequences: Primer pair 1: SEQ ID NO:1 and SEQ ID NO:2; Primer pair 2: SEQ ID NO:3 and SEQ ID NO:4. This invention employs polymerase chain reaction (PCR) to amplify specific gene fragments of *Trametes versicolor* by designing specific primers and amplification conditions, followed by detection by gel electrophoresis. This method can accurately identify samples containing *Trametes versicolor*, including medicinal materials, processed slices, and cultures. The identification results are accurate, and the method has high specificity, which is of great significance for ensuring the quality of *Trametes versicolor* pharmaceuticals and the safety of clinical applications.

The invention provides a Chinese herbal medicine feed additive for preventing and treating avian influenza and a preparation method thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The Chinese herbal medicine feed additive for preventing and treating avian influenza is prepared from the Chinese herbal medicines of isaria cicadae miq, iridis tectori, arisaema cum bile, solidago decurrens, radix pseudostellariae, coriolus versicolor and liquorice, is safe and non-toxic, can remarkably inhibit proliferation of H9N2 subtype AIV in chick embryos under in-vitro conditions, and has a remarkable antiviral effect. Meanwhile, the treatment effect on laying hens suffering from the avian influenza is remarkable, the death rate caused by the avian influenza is remarkably reduced, and the avian influenza can be effectively prevented. The Chinese herbal medicine feed additive disclosed by the invention has huge application potential in the aspects of treating and preventing avian influenza.

The application discloses a trametes versicolor polysaccharide (TV-P) and a preparation method and application thereof. The composition of the TV-P includes xylose, arabinose, glucose and galactose, and the molar ratio is 3:1:10:5. The TV-P is obtained by hot water extraction, ethanol precipitation, protein removal, ion exchange column chromatography, dialysis and concentration of the trama of trames versicolor. The TV-P has significant immunoregulatory activity and antitumor activity, and can be applied in medicines, health products or food.

This invention belongs to the field of daily chemical products and discloses a polysaccharide composition that improves skin immunity and strengthens the skin barrier function. The polysaccharide composition includes: (1) Scutellaria baicalensis polysaccharide, Adenophora stricta polysaccharide and Trametes versicolor polysaccharide; or (2) Scutellaria baicalensis polysaccharide, Lycium barbarum polysaccharide and Adenophora stricta polysaccharide; through the synergistic effect of the above three polysaccharides, it can not only reduce the number of mast cells, but also increase antimicrobial peptides; increase filaggrin; and reduce monocyte chemotactic protein-1 and interleukin-6, thereby achieving the effects of improving skin immunity, anti-inflammatory and whitening; it can also act as an information molecule to widely participate in physiological processes such as the mutual recognition of intercellular ligands and receptors and intercellular signal transduction, reduce the average relative amplification fold of interleukin-6 expression factor mRNA, and can participate well in the differentiation of epidermal cells and the formation of the skin barrier, thereby strengthening the skin barrier function; it can be used to prepare cosmetics.

The present invention relates to a vegan leather containing any one mushroom mycelium selected from the group consisting of fungi in the Polyporaceae family, the Boletaceae family, and combinations thereof. Specifically, the present invention relates to: a vegan leather containing a mycelium derived from Trametes versicolor belonging to the Polyporaceae family or Retiboletus nigerrimus belonging to the Boletaceae family; and a method for manufacturing same. According to the present invention, the vegan leather can be manufactured using a mushroom strain, which is an eco-friendly material, rather than artificial leather or animal leather.

The invention discloses a Trametes polyzona strain for degrading lignocellulose, the strain name of the Trametes polyzona strain is BY-TP7, the Trametes polyzona strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.41595. The invention further discloses a preparation method of the Trametes polyzona strain for degrading lignocellulose. According to the invention, the trametes multiflora strain capable of effectively degrading lignocellulose in stem and leaf parts or straws of the gramineous plants is separated for the first time, and the invention proposes that the trametes multiflora (or a fungicide or an additive containing the trametes multiflora) is added into the gramineous plants for aerobic fermentation for the first time. The content of lignocellulose in stem and leaf parts or straws of gramineous plants can be effectively reduced, and the fermentation quality is improved.

The invention discloses a method for interplanting pleurotus citrinopileatus under a macadamia nut forest. The method comprises the following steps: constructing a standardized cultivation-management circulating unit; waste of the macadamia nut industry is used as a core raw material, and the culture medium is prepared according to the dry weight volume; paving the culture medium into the culture furrows, and then inoculating pleurotus citrinopileatus cultispecies by adopting a layer sowing or mixed sowing mode; an orchard drip irrigation system is started for humidity management, and nutrition is supplemented in the mushroom cultivation period after each flush of mushrooms is harvested; when pleurotus citrinopileatus sporocarp pilei are bright golden yellow, timely harvesting by adopting a rotating pulling method; after 2-3 times of mushrooms are harvested, mushroom bran which is rich in hypha protein and completely decomposed in the cultivation furrows and covering soil are turned over and buried into the deep position of the orchard soil on the spot. According to the method, a forest-fungus interplanting mode is adopted, the under-forest space ecological niche and fruit shells, branches and other solid wastes generated by the method are integrated and utilized, large-scale and wild-imitating cultivation of pleurotus citrinopileatus is conducted, and collaborative symbiosis and cyclic development of cultivating fungi with forests and promoting forests with fungi are achieved.

The present application provides a new strain of Agaricus blazei Murill, which is Agaricus blazei Murill JSR-1 with a preservation number of CGMCC No.23250. The present application also provides Agaricus blazei Murill polysaccharide extracted from fermentation liquor of Agaricus blazei Murill JSR-1. The Agaricus blazei Murill strain with the preservation number of CGMCC No.23250 provided by the present application has a short preparation period, a high extraction rate, an easy-to-implement preparation method, and can be mass-produced. The Agaricus blazei Murill polysaccharide obtained by the method of the present application is safe and has no side effects, can obviously improve the water content of the skin, relieve skin inflammation, and has good soothing and anti-wrinkle effects.

The present application provides a kind of fermentation of Tricholoma mongolicum and Camellia nitidissima and its preparation method and application.The preparation method of the fermentation of Tricholoma mongolicum and Camellia nitidissima includes inoculating Tricholoma mongolicum liquid into fermentation substrate for fermentation culture, and then taking supernatant to obtain the fermentation of Tricholoma mongolicum and Camellia nitidissima.The fermentation substrate includes appropriately proportioned potato glucose liquid medium and Camellia nitidissima powder.The present application utilizes liquid fermentation of Tricholoma mongolicum and composition containing Camellia nitidissima powder with appropriate proportioning to achieve synergistic effect, especially selecting newly collected Tricholoma mongolicum strain with preservation number CGMCC No.23250, and under suitable conditions, fermentation of composite substrate mainly composed of fresh potato homogenate or filtrate or potato glucose water, Camellia nitidissima powder, soybean peptide and appropriate amount of glucose, to obtain well-performing composite fermentation product, which has good effects of promoting cell proliferation and anti-inflammation, is safe and has no side effects for cosmetics, has good skin feel, has good repair effect, can enhance skin elasticity, and can also be absorbed for a long time.

The present application relates to microbial fermentation technology, disclose a kind of cloud fungus, the fungicide containing the bacteria and its culture method and application.The cloud fungus ( Trametes versicolor ) of the present application provides the application of the cloud fungus in the fermentation production of active ingredients and / or the preparation of functional products, wherein the active ingredients include flavonoids and / or terpenoids.The fermentation performance of the cloud fungus is stable, the cost is low, the biomass and mycelium content are high, and it is rich in various flavonoids and terpenoids active ingredients.

This invention relates to the field of microbial fermentation technology, and particularly to a co-culture fermentation broth of medicinal and edible fungi with whitening effects, its preparation method, and its application. The preparation method of the co-culture fermentation broth of medicinal and edible fungi with whitening effects includes: inoculating the seed liquid of a first fungus and the seed liquid of a second fungus into a co-fermentation medium for fermentation and co-culture to obtain the co-culture fermentation broth of medicinal and edible fungi with whitening effects; the first fungus includes at least one of *Ganoderma lucidum* strain, *Trametes versicolor* strain, *Poria cocos* strain, *Auricularia auricula-judae* strain, *Phellinus linteus* strain, and *Aspergillus schavar*; the second fungus includes *Schizophyllum commune*. This invention co-cultures *Schizophyllum commune* with other specific strains and found that co-culturing the two strains can exert a synergistic effect, thereby significantly improving the tyrosinase inhibition effect of the fermentation broth. The optimal combination of fermentation broth can achieve a tyrosinase inhibition rate of over 90%, and even 1 wt% of the fermentation broth has a tyrosinase inhibition effect of approximately 90%.