159 results about "Pleurotus citrinopileatus" patented technology

The invention discloses a preparation method for an edible fungus flavor food. The edible fungus can be any one from edible agaricus bisporus, straw mushrooms, oyster mushrooms, shii-take, needle mushrooms, pleurotus nebrodensis, pleurotus eryngii, agrocybe aegerita, hypsizigus marmoreus, agaricus pratensis schaeff, spring mushrooms, stropharia rugoso-annulata, sparassis crispa, macrolepiota procera, tremellodon gelatinosum, black trumpet mushrooms, hericium erinaceus, pholiota nameko, lepista personata, mitake mushrooms, lactarius hatsudake, golden oyster mushrooms, pholiota adiposa, cantharellus cibarius, termitomyces albuminosus, tricholoma giganteum, pleurotus citrinopileatus, calocybe gambosa, truffle, amillariella mellea, fungus suillus, beef-steak fungus, lactarius deliciosus, tricholoma matsutake, agaricus bitorquis, pleurotus pulmonarius, toadstool and russula vinosa, and after treatment, the edible fungus is steeped into a steeping liquor prepared from red wine vinasse, high quality dry red wine and a flavouring agent in a vacuum and normal temperature and normal pressure combined condition, so as to obtain the flavor food; the edible fungus flavor food for leisure is prepared through oil-bath dewatering; a flavor food flavor puffed food is obtained through a swelling process, so as to meet consumers' hobbies and requirements.

The invention discloses a method for preparing an efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm). The method comprises the following steps: preparing a liquid or a solid culture medium from agricultural and sideline products and culturing fungi to obtain a bacterial liquid or a mycoplasm; carrying out physical and biological treatment on the bacterial liquid or the mycoplasm to enable polysaccharide polypeptide substances to be released from cells and adding a nitrogen source and a carbon source to prepare a probiotics culture medium; and then inoculating the probiotics and culturing to obtain the efficient probiotic preparation. The fungi comprise medicinal (edible) fungi of lentinu edodes, agrocybe cylindracea, morchella, pholiota nameko, hericium erinaceus, bolete, pleurotus nebrodensis, pleurotus citrinopileatus, lucid ganoderma, coriolus versicolor, cordyceps taishanensis, poria cocos, bamboo fungus, lactarius deliciosus and the like; and the probiotics is one or more selected from bacillus, saccharomycete, lactobacillus, enterococcus, bifidobacterium and the like. The probiotic preparation prepared by the method disclosed by the invention is rich in active substances, such as polysaccharides and polypeptides, has better health and treatment effects that those of a conventional probiotic preparation and can be used as therapeutic drugs and health foods or feed additives of human and animal gastrointestinal diseases.

The invention discloses pleurotus citrinopileatus sing ultrafine power cultured by using ramulus mori stems as major ingredients and a production method of the pleurotus citrinopileatus sing ultrafine power. The production method is characterized in that the ramulus mori stems is adopted as the major ingredients of the pleurotus citrinopileatus sing ultrafine power, 35-55 DEG C low-temperature vacuum drying is carried out for 4 to 5 hours, the crushing is carried out by a 500-1000-mesh sieve ultrafine crushing machine, and the pleurotus citrinopileatus sing ultrafine dry power is obtained. The pleurotus citrinopileatus sing ultrafine power and the production method have the advantages that the original nutrition and health care characteristics of pleurotus citrinopileatus sing can be maintained, the activity of B type monoamine oxidase is inhibited, in addition, the peroxyl lipid content of the liver can be reduced, the lipofuscin content of the heart is reduced, the activity of SOD (super oxygen dehydrogenises) in red blood cells can be improved, good effects of nourishing yin and yang, delaying senescence, preventing and resisting cancer, lowering the blood pressure and the like are realized, in addition, more convenient edible performance and efficient utilization performance of the health care ingredients are realized, and meanwhile, the silkworm industrial chain can be favorably prolonged.

The invention discloses a cultivation method of pleurotus citrinopileatus. The method comprises the steps as follows: treatment of biogas residues, preparation of a cultivation material, inoculation, spawning, fruiting and crop harvest, wherein the treatment of the biogas residues comprises the following steps: the biogas residues which are sufficiently fermented and decomposed, normally produce gas for more than three mouths and are free from fecal odor are stacked on a higher place for leaching, and the water content is 70%-80%; the preparation of the cultivation material comprises the following steps: proportioning, bagging and sterilization, the cultivation material comprises components in parts by weight as follows: 30-50 parts of biogas residues, 40-60 parts of ramulus mori scraps, 8 parts of corn flour, 1 part of lime and 1 part of gypsum powder; the sterilized cultivation material is cooled and inoculated; the inoculated cultivation material is cultivated for 25-30 days; fruiting is performed after a cultivation bag is overgrown with mycelia; a crop of pleurotus citrinopileatus is harvested before a fruiting body cap edge is spread but not involute; and fertilizer and water are appropriately supplemented after a crop of pleurotus citrinopileatus is harvested. The cultivation method of the pleurotus citrinopileatus utilizes the biogas residues for cultivating the pleurotus citrinopileatus, and has the advantages of wide material sources, convenience, simple technology, labor saving, time saving, material saving, low cost, good quality, high production, good benefit and the like.

The invention discloses a method for producing pleurotus citrinopileatus by using pleurotus eryngii substrate. The method comprises 1), crushing the pleurotus eryngii substrate into fine particles after removal of skins; 2), preparing a culture medium; and 3), sterilizing the culture medium. According to the method, the pleurotus citrinopileatus is produced by using pleurotus eryngii substrate, resources are saved, costs are reduced, pollution is reduced, and edible fungi are promoted to be on the path of eco-environmental protection and sustainable development.

The invention provides a pleurotus citrinopileatus cultivation material. The pleurotus citrinopileatus cultivation material is characterized in that pecan branch scraps, outer shell scraps and shell scraps are used as main raw materials, bagasse, rice bran, calcium superphosphate, sand plaster and the like are used as auxiliary materials, and the water content of the cultivation material is 63%-65%. The invention further provides a method for preparing the cultivation material. The pleurotus citrinopileatus cultivation material and the method have the advantages that the pecan branch scraps, the outer shell scraps and the shell scraps undergo sun exposure and secondary fermentation or piled ageing treatment, poisonous bacteriostat is degraded, macromolecular substances such as xylogen, cellulose and the like are decomposed into simple materials which can be absorbed by pleurotus citrinopileatus hyphae easily, and the growth of the pleurotus citrinopileatus hyphae is promoted; the cultivation material is reasonable in proportion, fungous materials are good in breathability and strong in water-retaining property, pleurotus citrinopileatus is good in growth and high in yield, and the biological efficiency of the pleurotus citrinopileatus reaches 130.5%-145.1%; a solution to innocent treatment of pecan branches, outer shells and shells is provided, and a novel matrix for pleurotus citrinopileatus cultivation is further found out.

The invention discloses a domestic fungus culture medium. The culture medium comprises, by weight, 15-20 parts of bean residues, 20-30 parts of rick husks, 15-25 parts of cassava stalk powder, 15-25 parts of spinach leaf powder, 10-15 parts of potatoes, 10-20 parts of corn flour, 5-10 parts of beef extract, 10-20 parts of guava leaves, 1-5 parts of vitamins, 2-8 parts of ammonium sulfate, 1-6 parts of gypsum, 1-3 parts of urea, 2-10 parts of Chinese yam powder, 2-10 parts of Spica Prunellae, 1-5 parts of iron citrate, 2-10 parts of silkworm chrysalis powder, 1-5 parts of licorice root and 1-6 parts of fermented chicken manure. The culture medium has the advantages of fast spawn running speed, short culture period, high output, low cost, simple composition, improvement of the output and the nutrition of edible fungi, enhancement of the disease resistance and the competitor infection resistance of the edible fungi, effective killing of competitors and viruses, guaranteeing of the healthy growth of the edible fungi, no pollution, no pesticide residual and low content of heavy metals, and is suitable for culturing Pleurotus eryngii, needle mushroom, Pleurotus citrinopileatus, Pleurotus ostreatus, Pleurotus nebrodensis, shiitake, edible tree fungus and various other edible fungi.

The invention relates to an edible fungus culture medium. The edible fungus culture medium comprises, by mass, 20-40 parts of corn straws, 10-30 parts of bran, 10-30 parts of rutin, 5-15 parts of light calcium carbonate, 5-15 parts of oilseed rape shell stems, 10-30 parts of tea residues, 5-10 parts of magnesium sulfate, 10-30 parts of rice bran and 5-10 parts of gibberellins. A preparation method of the edible fungus culture medium comprises the steps of prewetting and fermenting, material distributing of the edible fungus culture medium and bottling or bagging and sterilizing; a bottling machine or a bagging machine is adopted to pack the edible fungus culture medium in a split mode, the sterilizing and preparing are conducted, and a product is obtained. The edible fungus culture medium is suitable for edible funguses such as pleurotus eryngii, needle mushrooms, pleurotus citrinopileatus, oyster mushrooms, pleurotus nebrodensis, lentinula edodes and agarics, by adopting the edible fungus culture medium, the cultivation period can be shortened for 6-8 days, the yield is improved by 10-15%, and no apparent difference is shown in the physical form aspect.

The invention relates to a Pleurotus citrinopileatus culture material, which is characterized by using broad bean husk as the main raw material and cottonseed hull, wood dust, rice bran, bean pulp and the like as auxiliary materials, and the water content of the culture material is 63-65%. The invention also relates to a manufacturing method of the culture material. Compared with the wood dust culture material, the invention has the following advantages: the reasonable matching of rough and fine granules of the culture material enhances the air permeability and water retention property of the culture material, so that the growth speed of the Pleurotus citrinopicarbon hypha is enhanced, and the bagging time is shortened; the culture material has proper carbon-nitrogen ratio, thereby being beneficial to growth and development of sporocarps, and the yield is enhanced by 20-25%; and the invention widens the culture raw material, and greatly lowers the production cost. The invention has the following social benefits: by using the broad bean husk substitute material for culturing the Pleurotus citrinopileatus, the invention can relieve part of contradiction between bacterium and forest, and change wastes into valuable substances, thereby being beneficial to energy saving and emission reduction.

The invention relates to a Pleurotus citrinopileatus culture material which is characterized in that sunflower byproducts, including sunflower heads, sunflower straws, sunflower seed husks and sunflower seed cakes, are used as main raw materials; bran, sunflower seed cakes, calcium superphosphate, lime powder, gypsum powder and the like are used as auxiliary materials; and the water content of the culture material is 63-65%. The invention also relates to a manufacturing method of the culture material. The method has the following advantages: the raw materials are proportioned reasonably to promote the physical behavior complementation and nutrient complementation of the culture material, so that the culture material has favorable air permeability and high water retention property, and the Pleurotus citrinopileatus hypha can grow vigorously; the culture material has the advantages of balanced nutrition, good growth vigor of sporophores and high yield, and the biological efficiency is up to 140.6-148.3%; and the method provides a solution for innocent treatment of sunflower heads, sunflower straws and sunflower seed husks, and finds out a new substrate for culturing Pleurotus citrinopileatus.

The invention discloses a pleurotus citrinopileatus drink, which comprises the following components in part by weight: 80 to 120 parts of pleurotus citrinopileatus, 5 to 9 parts of shizandra berry, 2 to 6 parts of chrysanthemum, 50 to 70 parts of white granulated sugar and 700 to 900 parts of water. The pleurotus citrinopileatus drink is prepared by the following steps of: respectively extracting the pleurotus citrinopileatus, the shizandra berry and the chrysanthemum with water to form juice, combining the juice, adding a gelatine into the mixed juice, standing the mixture, centrifugating the mixture, taking the supernatant, adding the white granulated sugar into the supernatant, stirring, diluting with water, sterilizing and cooling. The pleurotus citrinopileatus drink is prepared from natural raw materials, is not added with any coloring matter and spice, is reasonable in compatibility, convenient to carry, safe, good in mouthfeel, rich in nutrition and nontoxic, has obvious health-care function and meets requirements of green food. The invention also discloses a preparation method for the pleurotus citrinopileatus drink.

The invention discloses a composition for treating diabetes and having the function of lowering blood sugar, and relates to a preparation method for the composition. The composition is prepared from the following components in percentage by weight: 25-40% of coprinus comatus mycelium, 8-15% of pleurotus citrinopileatus mycelium, 8-15% of grifola frondosa mycelium, 4-10% of Chinese caterpillar fungus mycelium, 3-10% of tricholoma matsutake mycelium, 3-10% of cypress mycelium, 3-10% of glossy ganoderma mycelium, 1-5% of fragrant mushroom mycelium, 1-5% of black fungus mycelium, 1-5% of needle mushroom mycelium, 3-10% of pholiota nameko mycelium, 1-5% of versicolor mushroom mycelium, and 1-4% of gastrodia tubers. The product disclosed by the invention has the effects of improving the immunity of human bodies, safety, health and lowering the blood sugar, and can be used for treating the diabetes.

The invention discloses a culture material for cultivating pleurotus citrinipileatus sing by using waste pleurotus eryngii. The culture material is prepared by mixing the following materials in percentage by mass: 30-65% of waste pleurotus eryngii, 5-15% of corncobs, 5-25% of saw dust, 5-20% of bran, 5-15% of corn flour and 2% of light calcium carbonate. When the culture material is used for cultivating the pleurotus citrinipileatus sing, not only is the utilization problem of the waste pleurotus eryngii solved, but also the raw material cost is greatly reduced, and the economic benefit brought to a producer is also increased; and the pleurotus citrinipileatus sing cultivated by using the culture material is yellow or bright yellow in body, beautiful in shape and white in pulp, and three crops of pleurotus citrinipileatus sing can be harvested.

The invention relates to a Pleurotus citrinopileatus stock compost which is characterized in that corn kernels are used as main raw materials, and cottonseed hulls and fine wood dust are used as auxiliary materials, wherein the water content of the corn kernels is 45-48%, and the water content of the wood dust is 62-63%. The invention also includes a preparation method of the compost. Compared with a wood dust compost, the compost provided by the invention has the following advantages: 1, the corn kernels are abundant in nutrition, hyphae can quickly grow, and the bottle filling time is shortened; 2, the corn kernel stock is uniform in granularity, the transplant rate (cultivated species) is high, and the strains germinate quickly and grow vigorously after transplant; and 3, the coarse and fine auxiliary materials are reasonably blended, so that the air permeability and water retention property of the compost are enhanced, thus preventing the corn kernels from age hardening and prolonging the storage life of the strains.

The invention discloses a pleurotus citrinopileatus culture medium and a preparation method thereof. The pleurotus citrinopileatus culture medium is prepared from the following raw material components in parts by weight: 30-40 parts of corn cobs, 25-35 parts of sawdust, 20-30 parts of pleurotus eryngii mushroom dreg, 12-15 parts of peanut cake meal, 9-14 parts of oil residue cakes, 6-12 parts of pineapple peel, 12-15 parts of sweet potato vine powder, 4-8 parts of celastrus angulatus, 2-4 parts of fructose, 13-18 parts of dry poultry manure, 3-7 parts of plant ash, 1-3 parts of gypsum powder and 5-10 parts of nutrition additives. The culture medium disclosed by the invention is wide in main material source, economical and environmentally friendly due to addition of the pleurotus eryngii mushroom dreg and easy in absorbing nutrient substances by thallus, so that the cost is lowered. Besides, the nutrition additives are added to improve the biotransformation efficiency of the pleurotus citrinopileatus, so that the trophic structure is enriched, and thus, the obtained mushrooms are relatively fresh and bright, and mellow.

The invention relates to a needle mushroom small molecule peptide compound solid beverage and a preparation method thereof, belongs to the technical field of processing of solid beverages, and provides a high-nutrient functional food unavailable at the market. Small molecule peptide powders including needle mushroom peptide powder, cauliflower peptide powder, brown rice peptide powder, kiwi fruitpeptide powder, chestnut mushroom peptide powder, ginseng peptide powder, toadstool peptide powder, abalone mushroom peptide powder, pleurotus citrinopileatus peptide powder, broad bean peptide powder, walnut peptide powder and mushroom peptide powder which have the effects of ensuring vigor, resisting fatigue and enhancing the memory, and honey which is capable of prolonging life are taken as main materials; polysaccharides, vitamins, trace elements, essential oil and the like are taken as auxiliary materials; and the main materials and the auxiliary materials are subjected to weighing, mixing in different proportions, granulating, drying and packaging to obtain peptide granules. The solid beverage provided by the invention has the effect of helping examinees to win the first prize.

The invention discloses a culture medium and method for cultivating pleurotus citrinopileatus through moxa residues (plant resides obtained after blumea camphor is extracted from blumea balsamifera (L.) DC. through steam distillation), and belongs to the technical field of edible mushrooms. The culture medium is prepared from, by weight, 40-50 parts of cotton seed hulls, 40-57 parts of moxa residues, 5-15 parts of wheat bran, 0.5-1.5 parts of land plaster, 0.5-1.5 parts of lime powder and 0.5-1.5 parts of saccharose. The method for cultivating the pleurotus citrinopileatus through the moxa residues comprises the steps of culture activation, production seed preparation, cultivating culture medium preparation, inoculating and spawn running. According to the cultivating culture medium, the moxa residues are used for replacing wood bits in a breakthrough mode, the nutritive value of the cultivated pleurotus citrinopileatus is consistent with that of the pleurotus citrinopileatu cultivatedthrough the wood bits, the production cost is reduced, and the economic benefits are effectively increased. Resources are recycled, waste is turned into wealth, production operation is easy to implement, high generalization performance is achieved, and the method is a feasible way in modern efficient agricultural development.

The invention discloses a solid beverage formed by compounding pleurotus nebrodensis with small molecule peptides and a preparation method of the solid beverage, belongs to the technical field of solid beverage processing and provides a high nutrition functional food in the market. By using collybia albuminosa peptide powder, pleurotus nebrodensis peptide powder, mushroom peptide powder, chestnutground peptide powder, hericium erinaceus peptide powder, coprinus comatus peptide powder, tremellodon gelatinosum peptide powder, pleurotus eryngii peptide powder, abalone mushroom peptide powder, pleurotus ostreatus peptide powder, pleurotus citrinopileatus peptide powder and wild portobello peptide powder, the effects of lowering blood pressure, blood fat and cholesterol can be achieved by thesmall molecule peptides. Honey is a long-lived food and the substances are selected as main materials; polysaccharides, vitamins, trace elements, essential oils and the like are taken as auxiliary materials; the main materials and the auxiliary materials are weighed and mixed according to different proportions and the mixture is prepared into granules; and the granules are dried and packaged to obtain peptide granules. The solid beverage has the efficacy of lowering the high blood pressure, the high blood fat and the cholesterol.

The invention discloses a Pleurotus citrinopileatus protein extractive and an application thereof in tumor resisting and particularly relates to the application of the Pleurotus citrinopileatus protein extractive in preparation of anti-tumor products or anti-tumor cell products. The Pleurotus citrinopileatus protein extractive is prepared by a method including the following steps of: (1) carrying out lixiviating by utilizing water after crushing Pleurotus citrinopileatus sporocarp, collecting water-soluble matters, so as to obtain Pleurotus citrinopileatus water-soluble extractives; and (2) carrying out ammonium sulfate precipitation with the saturability of 80% on the Pleurotus citrinopileatus water-soluble extractives, collecting precipitates, and obtaining the Pleurotus citrinopileatus protein extractive after carrying out deionized water dialysis on the precipitates. After the Pleurotus citrinopileatus protein extractive is used for processing human liver cancer HepG cells, human breast cancer MCF-7 cells and human lung cancer A-549 cells for 72 hours, IC50 (half maximal inhibitory concentration) values respectively are 1.0 microgrammes per milliliter, 0.45 microgrammes per milliliter and 1.15 microgrammes per milliliter, and inhibition ratios are increased along the prolonging of the time and the increasing of the concentrations.

The invention relates to a fungal polysaccharide and a composition thereof with anti-gastric ulcer effects. According to the invention, a crude polysaccharide is obtained from fruiting bodies of pleurotus eryngii, white beech mushroom, calocybe gambosa, pleurotus citrinopileatus and hypsizygus mammoreus bigelow and the crude polysaccharide is purified with a purification method adopting a mixed pillar consisting of 717 cation exchange resin and 732 anion exchange resin; the anti-gastric ulcer effect of the purified fungal polysaccharide and the composition thereof is obviously strengthened; meanwhile, substances which possibly stimulate an immune system to generate allergy are removed, so that the fungal polysaccharide and the composition thereof are safer to use and are also beneficial to quality control of a product. According to the invention, the fungal polysaccharide and the composition thereof are proved to have obvious effects of preventing and treating gastric ulcer by experiments for the first time.

The invention relates to a method for cultivating and culturing pleurotus citrinopileatus by using forestry waste persimmon sawdust as main material. The method comprises a breeding process of pleurotus citrinopileatus capable of decomposing the persimmon sawdust and belongs to the technical field of utilization of forestry residues and cultivation of edible and medicinal funguses. The method comprises the following steps of: A, screening a pleurotus citrinopileatus strain having the decomposition advantage to the persimmon sawdust; B, propagating the strain; and C, cultivating the pleurotus citrinopileatus strain. According to the method, in ultraviolet mutation breeding and primary screening steps of the pleurotus citrinopileatus, persimmon sawdust powder is used as an only carbon source to induce and screen the strain which can effectively utilize the persimmon sawdust, so that the breeding efficiency of decomposition strain is improved; and during preparation of liquid strains, the persimmon sawdust is used as a specific substrate corresponding enzymes are generated because of induction function, adaptation time of the strain on a culture medium which uses the persimmon sawdust as the main material is shortened, and the fungus generation speed is improved, so that a technical scheme that the forestry waste persimmon sawdust is effectively utilized by breeding the pleurotus citrinopileatus strain which can efficiently use the persimmon sawdust is provided.

The invention discloses a pleurotus citrinopileatus cultivation method and relates to the technical field of cultivation. The pleurotus citrinopileatus cultivation method comprises the steps that firstly, piling and fermenting are conducted on culture media; secondly, the fermented culture media are bagged, sterilized, inoculated and cultivated; thirdly, after bags are full of hyphae and are kept for three days, the fungus bags are moved to a mushroom house, the temperature of the mushroom house is controlled within the range from 23 DEG C to 27 DEG C, and the day and night temperature difference is controlled within the range from 7 DEG C to 8 DEG C; fourthly, after fruiting, the air relative humidity is adjusted to the range from 85 percent to 95 percent, a user should pay attention to ventilation, and the concentration of carbon dioxide is kept below 500 ppm; fifthly, pleurotus citrinopileatus can be harvested when the edges of pilei of fruiting bodies are expanded to the maximum degree or are in a small wave shape. Compared with the prior art, mulberry branches serve as the main material of the culture media, can be got easily and is low in price, the nutrition value of the cultivated pleurotus citrinopileatus is the same as the nutrition value of pleurotus citrinopileatus cultivated through cottonseed hulls.

The invention discloses application of momordica grosvenori pomace to cultivating pleurotus citrinopileatus. Momordica grosvenori pomace powder is mixed with cottonseed hulls, bagasse or corncob, bran, land plaster and lime powder so as to prepare composts for cultivating the pleurotus citrinopileatus. The new cultivating raw materials for cultivating the pleurotus citrinopileatus are provided, and the high yield and the high quality of the pleurotus citrinopileatus can be achieved; in addition, waste can be used, and waste recycling is achieved.

The invention discloses a pleurotus citrinopileatus culture medium based on eupatorium adenophorum. The pleurotus citrinopileatus culture medium is prepared from components in parts by weight as follows: ageratina adenophora, wood vinegar, seaweed polysaccharides, soluble swelling starch, peptone, nanoscale tourmaline powder, bacterial nanocellulose and the like. A pleurotus citrinopileatus liquid strain is prepared for the culture medium in combination with the ageratina adenophora, the soluble swelling starch and the like; the sources of materials are wide, and the price cost is low; besides, the ageratina adenophora is subjected to super-fine grinding and then mixed with the seaweed polysaccharides and the wood vinegar, the mixture is boiled and extracted with soft fire, and the ingredients of the culture medium are enriched; meanwhile, the stability of the liquid culture medium is promoted by means of the property of the seaweed polysaccharides, and rich nutrition and good space are provided for stock culture and expanding culture of pleurotus citrinopileatus; good nutrients and living environments are provided for mycelium pellets of the pleurotus citrinopileatus liquid strain due to addition of the nanocellulose, the distribution stability of mycelium pellets can be improved, and loss caused by mechanical pulverization of the mycelium pellets before the liquid strain is used is avoided.

The invention provides a preparation method of pleurotus citrinopileatus amino acid powder and belongs to the technical field of preparation of amino acid. The preparation method of the pleurotus citrinopileatus amino acid powder comprises washing, pulping and enzymolysis filtration, wherein enzymatic hydrolysate is subjected to filtration with a 100-mesh sieve, ultrafiltration, flash evaporation concentration, spray drying and the like. With adoption of techniques of breaking assisted by ultrasonic waves as well as enzymolysis, breaking of cell walls of pleurotus citrinopileatus is facilitated, so that nutritional ingredients can be dissolved out sufficiently, protein in pleurotus citrinopileatus can be sufficiently hydrolyzed to amino acid and small molecule peptides, and accordingly, the protein in pleurotus citrinopileatus can be conveniently absorbed by a human body. With adoption of the flash evaporation concentration and spray drying techniques, microcapsulated pleurotus citrinopileatus amino acid powder is free of residual solvents, good in embedding effect and higher in yield.

The invention relates to the field of fungus cultivation, in particular to a pleurotus citrinopileatus culture medium preparation method and a pleurotus citrinopileatus cultivation method. The pleurotus citrinopileatus culture medium preparation method includes steps: taking each component for standby application according to culture materials; grinding kenaf stalks, and adding water to prewet along with saw dust; mixing other components with the pre-wetted kenaf stalks and saw dust, adding water, and well mixing to obtain a wet material in water content of 65%-68%; adding the wet material into a container, and sterilizing to obtain a pleurotus citrinopileatus culture medium. According to the pleurotus citrinopileatus cultivation method, the kenaf stalks are added to serve as a main material of the culture medium, proportions of auxiliary materials such as bran, corn flour and the like are increased, liquid strains are adopted for industrial bottle cultivation, and applying the methodfor pleurotus citrinopileatus cultivation realizes uniformity in fruiting mainly concentrated on first three rounds in an explosive fruiting characteristic; short production period, high biological efficiency, low cost and the like can be realized, seasonal and regional limitations are avoided, and year-round production of pleurotus citrinopileatus is realized.

The invention discloses an edible fungus culture medium. The raw materials of the culture medium include, by weight, 20-40 parts of hard mixed wood chips, 10-15 parts of bagasse, 5-15 parts of bean dregs, 10-30 parts of sodium hydrogen phosphate, 5-15 parts of camelina sativa cake, 5-15 parts of kelp residue, 10-30 parts of cotton seed hulls, 5-10 parts of an ionized calcium solvent, 10-30 parts of outer skin of a bamboo shoot, 5-10 parts of gibberellin, 1-5 parts of vitamins, 2-8 parts of ammonium sulfate, 2-10 parts of yam powder, 2-10 parts of common self-heal fruit-spike, 1-5 parts of ferric citrate, 2-10 parts of silkworm chrysalis powder, 1-5 parts of liquorice root, and 1-6 parts of fermented chicken manure. Through the culture medium, cultivation period can be shortened, yield canbe enhanced, the nutrition raw materials in the medium can be enriched, and full supply of the raw materials needed by strains can be guaranteed; and the culture medium is suitable for the edible fungus culturing of various edible fungi like pleurotus eryngii, flammulina velutipes, pleurotus citrinopileatus, oyster mushroom, pleurotus nebrodensis, shiitake mushroom, auricularia auricula-judae, etc.

The invention discloses a method for preparing an edible fungus protein extract with antitumor efficacy. The preparation method comprises the following steps of: 1) extracting by water after crushing edible fungus sporocarp, collecting a water-soluble matter to obtain an edible fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the edible fungus water-soluble extract, collecting sediments, and obtaining the edible fungus protein extract with the antitumor efficacy after dialyzing the sediments by deionized water, wherein the edible fungus is at least one of the following funguses: auricularia polytricha, pleurotus citrinopileatus, abalone mushroom, pycnoporus cin, cordyceps militaris, pleurotus djamor, black fungus, clitocybe maxima, flammulina velutipes, oudemansiella radicata and pholiota adipose. The edible fungus protein extract disclosed by the invention has a significant proliferation inhibition role on an HepG2 cell, an MCF-7 cell and an A-549 cell, and can be used for preparing a product for resisting tumors or tumor cells.