36results about How to "Significant inhibition of proliferation" patented technology

The invention relates to the field of genetic engineering, in particular to a novel fungal immunomodulatory protein FIP-NHA with antineoplastic activity and a gene thereof. The fungal immunomodulatory protein FIP-NHA according to the invention has an amino acid sequence as shown in SEQ ID NO. 1, and the gene fip-nha for coding the fungal immunomodulatory protein has a nucleotide sequence as shownin SEQ ID NO. 2. The fungal immunomodulatory protein FIP-NHA provided by the invention has the effects on promoting splenic lymphocyte division, increasing synthesis of interleukin 2, and obviously inhibiting proliferation of three tumor cells including hepatoma cell HepG2, gastric cancer cell MGC823 and leukemia cell HL60, therefore, the fungal immunomodulatory protein FIP-NHA as a novel antineoplastic biological agent can be widely used in the fields of health products and biological medicines.

The invention discloses 2-(3,4-dimethoxy)benzoyl-5-(4-substituted phenylethynyl)thiophene, whose structural formula is as follows: ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ; Its preparation method comprises steps: 1) In the presence of a catalyst, react 2-bromothiophene and 3,4-dimethoxybenzoyl chloride in a solvent, and subsequently purify and separate to obtain 2-(3,4-dimethoxy Methoxy) benzoyl-5-bromothiophene; 2) In the presence of tetrakis (triphenylphosphine) palladium, CuI, acid binding agent, 2-(3,4-dimethoxy) benzoyl- 5-bromothiophene and 4-substituted phenylacetylene are reacted in a solvent, followed by subsequent purification and separation. The application of the compound in the preparation of anti-mammary cancer targeted therapy drugs. The application of the compound in the preparation of an anti-breast cancer drug resistance reversal agent. The compound of the present invention has obvious anti-proliferation effect on human breast cancer cells and drug-resistant strains of breast cancer cells, and can be used to prepare anti-breast cancer targeted therapy drugs and anti-breast cancer drug resistance reversal agents. Moreover, the compound of the present invention has the potential for large-scale industrial production, and the cost is relatively low.

The invention discloses a pycnporus cinnabarnus protein extract and anti-tumor application thereof. The pycnporus cinnabarnus protein extract is prepared according to the following steps of: 1) extracting by water after crushing pycnporus cinnabarnus sporocarp, and collecting water-soluble substances to obtain a pycnporus cinnabarnus water-soluble extract; 2) carrying out ammonium sulfate precipitation on the pycnporus cinnabarnus water-soluble extract of which the saturation level is 80%, collecting sediments, thereby obtaining the pycnporus cinnabarnus protein extract after dialyzing the sediment by deionized water. The IC50 values are 12 mu g / ml, 6 mu g / ml and 4 mu g / ml respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a human lung adenocarcinoma A-549 cell are processed by the pycnporus cinnabarnus protein extract; the inhibition ratio is increased along with time expansion and increase of the concentration; apoptosis morphology change of the cells can be observed under a light microscope.

The invention discloses a pholiota adiposa protein extract and anti-tumor application thereof. The pholiota adiposa protein extract is prepared according to the method comprising the following steps of 1) extracting by water after crushing pholiota adiposa sporocarp, collecting a water-soluble matter to obtain a pholiota adiposa water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the pholiota adiposa water-soluble extract, collecting sediments, and obtaining the pholiota adiposa protein extract after dialyzing the sediments by deionized water. IC50 values are 30 mug / mL, 20 mug / mL, and 20 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the pholiota adiposa protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.

The invention discloses a black fungus protein extract and anti-tumor application thereof. The black fungus protein extract is prepared according to the method including the following steps of 1) extracting by water after crushing black fungus sporocarp, collecting a water-soluble matter to obtain a black fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the black fungus water-soluble extract, collecting sediments, and obtaining the black fungus protein extract after dialyzing the sediments by deionized water. IC50 values are 6 mug / mL, 6 mug / mL, and 6 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the black fungus protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.