36results about How to "Significant inhibition of proliferation" patented technology

The invention relates to the field of genetic engineering, in particular to a novel fungal immunomodulatory protein FIP-NHA with antineoplastic activity and a gene thereof. The fungal immunomodulatory protein FIP-NHA according to the invention has an amino acid sequence as shown in SEQ ID NO. 1, and the gene fip-nha for coding the fungal immunomodulatory protein has a nucleotide sequence as shownin SEQ ID NO. 2. The fungal immunomodulatory protein FIP-NHA provided by the invention has the effects on promoting splenic lymphocyte division, increasing synthesis of interleukin 2, and obviously inhibiting proliferation of three tumor cells including hepatoma cell HepG2, gastric cancer cell MGC823 and leukemia cell HL60, therefore, the fungal immunomodulatory protein FIP-NHA as a novel antineoplastic biological agent can be widely used in the fields of health products and biological medicines.

The invention discloses an application of cycloartane triterpenoid (23Z)-9,19-cycloartane-23-alkene-3alpha, 25-glycol in preparation of an anti-lung cancer medicine. The plant source of cycloartane triterpenoid is abundant; when the effective in vitro acting concentration of the prepared cycloartane triterpenoid (23Z)-9,19-cycloartane-23-alkene-3alpha, 25-glycol for a lung cancer cell strain is 5-20mu mol / L, the cycloartane triterpenoid has obvious proliferation inhibition action on the lung cancer cell strains H1299, H1944, H292 and H358 and the IC50 values are respectively 2.6mu mol / L, 7.8mu mol / L, 4.9mu mol / L and 8.7mu mol / L. The cycloartane triterpenoid can be applied to preparation of the anti-lung cancer medicine.

The invention discloses 2-(3,4-dimethoxy)benzoyl-5-(4-substituted phenylethynyl)thiophene, whose structural formula is as follows: ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ; Its preparation method comprises steps: 1) In the presence of a catalyst, react 2-bromothiophene and 3,4-dimethoxybenzoyl chloride in a solvent, and subsequently purify and separate to obtain 2-(3,4-dimethoxy Methoxy) benzoyl-5-bromothiophene; 2) In the presence of tetrakis (triphenylphosphine) palladium, CuI, acid binding agent, 2-(3,4-dimethoxy) benzoyl- 5-bromothiophene and 4-substituted phenylacetylene are reacted in a solvent, followed by subsequent purification and separation. The application of the compound in the preparation of anti-mammary cancer targeted therapy drugs. The application of the compound in the preparation of an anti-breast cancer drug resistance reversal agent. The compound of the present invention has obvious anti-proliferation effect on human breast cancer cells and drug-resistant strains of breast cancer cells, and can be used to prepare anti-breast cancer targeted therapy drugs and anti-breast cancer drug resistance reversal agents. Moreover, the compound of the present invention has the potential for large-scale industrial production, and the cost is relatively low.

The invention discloses an auricularia polytricha protein extract and anti-tumor application thereof. The application is application of the auricularia polytricha protein extract in preparation of a product for restricting tumor cell proliferation. The auricularia polytricha protein extract is prepared according to the method comprising the following steps of: 1) extracting by water after crushing auricularia polytricha sporocarp, collecting a water-soluble matter to obtain an auricularia polytricha water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the auricularia polytricha water-soluble extract, collecting sediments, and obtaining the auricularia polytricha protein extract after dialyzing the sediments by deionized water. Multiplication of HepG2, MCF-7 and A-549 cells is obviously restrained after the human liver cancer HepG2 cell, the human breast cancer MCF-7 cell and the pulmonary adenocarcinoma A-549 cell are processed by the auricularia polytricha protein extract for 72 hours; the IC50 values are 45 mug / mL, 20 mug / mL, and 25 mug / mL respectively.

The invention discloses a pycnporus cinnabarnus protein extract and anti-tumor application thereof. The pycnporus cinnabarnus protein extract is prepared according to the following steps of: 1) extracting by water after crushing pycnporus cinnabarnus sporocarp, and collecting water-soluble substances to obtain a pycnporus cinnabarnus water-soluble extract; 2) carrying out ammonium sulfate precipitation on the pycnporus cinnabarnus water-soluble extract of which the saturation level is 80%, collecting sediments, thereby obtaining the pycnporus cinnabarnus protein extract after dialyzing the sediment by deionized water. The IC50 values are 12 mu g / ml, 6 mu g / ml and 4 mu g / ml respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a human lung adenocarcinoma A-549 cell are processed by the pycnporus cinnabarnus protein extract; the inhibition ratio is increased along with time expansion and increase of the concentration; apoptosis morphology change of the cells can be observed under a light microscope.

The invention discloses a nitrogen-containing fused heterocycle compound shown by a formula I, a pharmaceutically acceptable salt thereof, or enantiomers, diastereoisomers, tautomers, solvates, metabolic precursors or prodrugs of the nitrogen-containing fused heterocycle compound and the pharmaceutically acceptable salt thereof, or a pharmaceutical composition containing the nitrogen-containing fused heterocycle compound and an application of the nitrogen-containing fused heterocycle compound. The nitrogen-containing fused heterocycle compound disclosed by the invention has relatively good antitumor activity.

The invention discloses a pholiota adiposa protein extract and anti-tumor application thereof. The pholiota adiposa protein extract is prepared according to the method comprising the following steps of 1) extracting by water after crushing pholiota adiposa sporocarp, collecting a water-soluble matter to obtain a pholiota adiposa water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the pholiota adiposa water-soluble extract, collecting sediments, and obtaining the pholiota adiposa protein extract after dialyzing the sediments by deionized water. IC50 values are 30 mug / mL, 20 mug / mL, and 20 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the pholiota adiposa protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.

The invention discloses a nitrogen-containing condensed heterocyclic compound, its preparation method, intermediate, composition and application. The compound of the present invention has higher inhibitory activity on different subtypes of CDK at the molecular level, better inhibitory activity on breast cancer cells at the cellular level, and also has inhibitory activity on tumor cells related to cyclin-dependent kinase activity at the animal level Significant inhibitory effect on proliferation, good stability to liver microsomes of humans and mice, no obvious inhibition of metabolic enzymes, good absorption properties in rats and mice, high bioavailability, and good druggability.

The invention discloses a black fungus protein extract and anti-tumor application thereof. The black fungus protein extract is prepared according to the method including the following steps of 1) extracting by water after crushing black fungus sporocarp, collecting a water-soluble matter to obtain a black fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the black fungus water-soluble extract, collecting sediments, and obtaining the black fungus protein extract after dialyzing the sediments by deionized water. IC50 values are 6 mug / mL, 6 mug / mL, and 6 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the black fungus protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.