43 results about "Oudemansiella radicata" patented technology

The invention discloses a method for artificially cultivating Oudemansiella radicata var.alba, which is specific application of a biological technology for artificial acclimatization and cal solve the difficult technical problem in artificial cultivation of wild fungi. The technical method comprises the following steps of: making a cultivation matrix recipe; preparing a cultivation matrix in a cultivation manner; and cultivating the cultivation matrix on a soil-covered bed, or directly covering soil at a bag opening for growing the Oudemansiella radicata var.alba. The method provided by the invention can be used for producing the Oudemansiella radicata var.alba through the artificial cultivation.

The deep fermentation process uses the spawn of Oudemansiella radicata from Central-China Agricultural Univ. and includes the following steps: slant spawn culture, shake flask seed culture, 7L fermenting tank culture, and polysaccharide extraction and determination. The present invention selects corn powder and soybean cake powder as main fermentation material and has low production cost. It is found that palmitic acid is key nutritious additive factor capable of raising the yield of extracellular polysaccharide of Oudemansiella radicata up to 30-40 %. The fermentation process is controlled through on-line control of variation in dissolved oxygen concentration. The present invention can realize large scale fermentation with simple technological operation, and is suitable for industrial production.

The invention discloses an application of siraitia grosvenorii fruit residues in cultivation of oudemansiella radicata. Siraitia grosvenorii fruit residue powder is mixed with cottonseed hulls, bran, gypsum powder and calcium carbonate to prepare compost for cultivating the oudemansiella radicata. According to the application, one novel cultivation raw material for cultivating the oudemansiella radicata is provided, high yield and high quality of the oudemansiella radicata can be achieved, and further, utilization and resource recovery of waste are realized.

The invention discloses a method for preparing an edible fungus protein extract with antitumor efficacy. The preparation method comprises the following steps of: 1) extracting by water after crushing edible fungus sporocarp, collecting a water-soluble matter to obtain an edible fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the edible fungus water-soluble extract, collecting sediments, and obtaining the edible fungus protein extract with the antitumor efficacy after dialyzing the sediments by deionized water, wherein the edible fungus is at least one of the following funguses: auricularia polytricha, pleurotus citrinopileatus, abalone mushroom, pycnoporus cin, cordyceps militaris, pleurotus djamor, black fungus, clitocybe maxima, flammulina velutipes, oudemansiella radicata and pholiota adipose. The edible fungus protein extract disclosed by the invention has a significant proliferation inhibition role on an HepG2 cell, an MCF-7 cell and an A-549 cell, and can be used for preparing a product for resisting tumors or tumor cells.

The invention belongs to the field of medicines, and discloses amino sugar oral liquid and a preparation method thereof. The amino sugar oral liquid comprises the components in parts by weight of 40-50 parts of glucosamine, 10-16 parts of oudemansiella radicata polysaccharide, 8-15 parts of raspberry polysaccharide, 5-7 parts of mint essence, 2-6 parts of a preservative, 7-15 parts of a solubilizer, 1-3 parts of a complexing stabilizing agent and 1-6 parts of a corrigent, wherein the pH is regulated to 5.5-7 with a pH-regulating agent. The amino sugar oral liquid contains the oudemansiella radicata polysaccharide having the medicine and pharmacology activity, and the raspberry polysaccharide having the efficacy of reducing blood sugar and resisting weariness, not only has the nutrient andhealth-care functions, but also is high in medical value and good in stability.

The invention provides a mother culture medium for artificial cultivation of oudemansiella radicata. The culture medium consists of the following components in parts by weight: 200 parts of potatoes, 20-25 parts of sugar, 18-20 parts of corn meal, 2-3 parts of soybean meal, 4-5 parts of algin, 0.2-0.3 part of vitamin, 1 part of monopotassium phosphate, 0.5 part of magnesium sulfate, 8-10 parts of coconut juice, 0.001 part of kinetin and 1000 parts of water. The invention also provides a preparation method of the mother culture medium for artificial cultivation of oudemansiella radicata. The mother culture medium disclosed by the invention is relatively high in biological efficiency and can be used for effectively improving the yield of oudemansiella radicata.

The invention discloses a cultivation method of an oudemansiella radicata strain. According to the cultivation method, an edible fungus cultivation device is used for cultivating the oudemansiella radicata strain, the edible fungus cultivation device provides proper cultivation conditions for the oudemansiella radicata strain through a vibration cultivation mechanism, and manual cultivation is replaced with mechanical equipment cultivation, so that the culture efficiency and accuracy of the oudemansiella radicata strain are effectively improved; a porous culture medium carrier is prepared in the strain culture process, and a scientific and reasonable strain culture medium is used in cooperation, so that artificial culture of the oudemansiella radicata strain is achieved, various types of bioactive substances are contained in strain culture, a promoting effect on formation of oudemansiella radicata mycelium primordia and growth of fruiting body mycelia, and oudemansiella radicata cultured by the cultured oudemansiella radicata strain has the advantages of thick stipe, bright color, fleshy flesh, high environmental adaptability, high spawn running speed, short production period and stable yield.

The invention discloses an oudemansiella radicata three-dimensional cultivation bed frame capable of automatically ascending and descending. The cultivation bed frame comprises a flat plate; a wide plate and a narrow plate are fixedly connected with the two sides of the upper end of the flat plate respectively; a lifting plate is arranged at the upper end of the side, close to the narrow plate, of the wide plate; a plurality of cultivation units are arranged between the wide plate and the narrow plate at equal intervals; each cultivation unit comprises a bearing plate; a weighing device is fixedly mounted at the upper ends of the bearing plates; a cultivation box is fixedly and horizontally arranged at the upper end of the weighing device; a bearing net is horizontally and fixedly connected with the inner wall of the cultivation box; a left sliding plate and a right sliding plate are fixedly connected with the left side and the right side of the cultivation box respectively; and a left sliding plate groove in sliding connection with the left sliding plate and a right sliding plate groove in sliding connection with the right sliding plate are formed in the opposite sides of the wide plate and the narrow plate correspondingly. According to the oudemansiella radicata three-dimensional cultivation bed frame capable of automatically ascending and descending, the multiple cultivation units are driven to stretch or retract through linkage of connecting rods, more equipment resources are saved, water can be supplemented in time, and a proper humidity environment is provided for oudemansiella radicata.

The invention discloses an oudemansiella radicata protein extract and anti-tumor application thereof. The oudemansiella radicata protein extract is prepared according to the method comprising the following steps of: 1) extracting by water after crushing oudemansiella radicata sporocarp, collecting a water-soluble matter to obtain an oudemansiella radicata water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the oudemansiella radicata water-soluble extract, collecting sediments, and obtaining the oudemansiella radicata protein extract after dialyzing the sediments by deionized water. IC50 values are 5 mug / mL, 7 mug / mL, and 8 mug / mL respectively after a human liver cancer HepG2 cell, a human breast cancer MCF-7 cell and a pulmonary adenocarcinoma A-549 cell are processed by the oudemansiella radicata protein extract for 72 hours; the inhibition ratio is increased along with time extension and increase of the concentration; apoptosis morphological changes of the cells can be observed under a light microscope.

Use of fruit skins and pruned branches and tendrils of Passiflora edulia in Oudemansiella radicata cultivation is characterized in that smashed Passiflora edulia fruit skin powder and pruned branch and tendril powder are evenly mixed to be as a main carbon source of a cultivation medium, and the mixture is used for Oudemansiella radicata cultivation. The test shows that the fruit skins and pruned branches and tendrils of the Passiflora edulia are used to replace other expensive carbon sources such as cotton seed hulls, and the mixture as the carbon source is used to prepare the cultivation medium to cultivate the Oudemansiella radicata. The yield and quality of the obtained Oudemansiella radicata is substantially identical to that of the Oudemansiella radicata cultivated by using the conventional cultivation mediums, the production cost is reduced, the economic benefits are increased, waste materials can be reused, and recycling of the waste materials can be achieved.

The invention discloses a compound oudemansiella radicata polysaccharide oral liquid. The compound oudemansiella radicata polysaccharide oral liquid is prepared from, by mass, 5%-20% of oudemansiella radicata polysaccharides, 1%-10% of milkvetch roots, 1%-10% of poria cocos, 1%-10% of fructus schisandrae chinensis polysaccharides, 0.1%-3% of acid agent, 3%-15% of sweetening agent and the balance water. The invention further discloses a preparation method of the oral liquid. The oral liquid has the health-care functions such as oxidation resistance and body immunity enhancement, technological operation is easy and convenient, the cost price is low, and the oral liquid is suitable for industrial production.

The present invention discloses an efficient oudemansiella radicata culture medium with propolis being antibacterial and nutrient substances. The culture medium comprises the following components in parts by weight: macroporous thermally-degradable water-soluble polyvinyl alcohol microspheres, porous ultra-high-strength activated carbon microspheres, porous chitosan microspheres, rice roots, perilla oil cakes, germinated brown rice, castor oil, Hedysari polysaccharide, ionic liquid emulsified propolis and yeast. According to the culture medium provided by the present invention, porous ultra-high-strength activated carbon microspheres, porous chitosan microspheres and ionic liquid emulsified propolis are used to prepare the modified latex which is used as nutrient and antibacterial substances added into the culture medium, so that nutritive and antimicrobial effects of the culture medium are improved, thereby achieving natural and environmentally-friendly features. Macroporous thermally-degradable water-soluble polyvinyl alcohol microspheres are used to adsorb the loaded yeast for fermentation, so that the fermentation rate is effectively adjusted; decomposition of the fermentable substrate is promoted; nutrients of the culture medium are more abundant, more balanced and more convenient for absorption and utilization by the oudemansiella radicata; and a basis is provided for efficient cultivation of the oudemansiella radicata.

The invention provides a high-yield Oudemansiella radicata cultivation material prepared from corn cob and radix puerariae residue, wherein the cultivation material is prepared from the following components by weight: 40-45 parts of corn cob, 40-45 parts of radix puerariae residue, 13-16 parts of bran, 0.8-1.2 parts of sucrose, and 0.8-1.2 parts of calcium carbonate. According to the present invention, the problems of less source and high prices of the carbon source main material for the Oudemansiella radicata cultivation are well solved.

The invention discloses a method for preparing oudemansiella radicata culturing medium by using crop byproducts. The crop byproducts of peanut shells, mulberry twigs and corn stalk are taken as main raw materials, the problem of the conversion of crop wastes is solved, new raw material sources are provided for oudemansiella radicata culturing, the economic benefits of the crop byproducts and the comprehensive utilization of resources are improved, the production cost can be reduced, and the economic benefits can be increased. According to the method, bean pulp, humic acid powder and urea are taken as nutritional agents, mineral salt is antimicrobial, monopotassium phosphate and pulverized lime are auxiliaries, comprehensive allocation is conducted to obtain a culturing medium with good activity, loosened structure, good air permeability, strong water preserving performance and good hybrid bacteria resisting performance, the growing demands of the oudemansiella radicata are met, and theoudemansiella radicata with good quality is obtained.

The invention discloses compost of oudemansiella radicata and application of the compost as cover soil. The compost is mainly prepared from cassava peel, agricultural pruning branches, bagasse, corn flour, light calcium carbonate, quicklime powder and potassium sorbate, the sources of the ingredients are wide, the ingredients are obtained easily, the price is low, not only is the reuse of waste resources achieved, but also the nutritional ingredients can be provided for the growth of oudemansiella radicata; the potassium sorbate is added to prevent too many microorganisms and sundry fungi fromgrowing; the compost of oudemansiella radicata replaces traditional cover soil, not only can a cover material through high temperature sterilization be obtained, but also the cost can be reduced, therisk of secondary risk during covering fruiting, thereby obtaining fresh, cool and clean oudemansiella radicata with good overall quality, so that oudemansiella radicata planting has better economicbenefits.

The invention belongs to the technical field of resources and environment, and relates to a method for jointly repairing heavy metal-contaminated soil by root mushrooms and golden blessing mushrooms. The method is to plant root mushrooms and golden blessing mushrooms on the polluted soil at the same time. When the bacteria are mature, harvest the mushrooms The mushroom body is processed to obtain a mushroom slurry rich in heavy metal ions, and then the heavy metal ions in the mushroom slurry are removed by chemical electrophoresis or chelation precipitation. The present invention takes the long root mushroom and golden blessing mushroom as the main bioconcentrators, and fully utilizes the characteristics that the two kinds of mushrooms can grow under similar soil conditions at the same time, the growth period is short, the field cultivation technology is mature, and the ability to enrich heavy metal ions is strong. Combined with simple and mature chemical methods such as chemical electrophoresis or chelation precipitation, heavy metal ions can be completely removed from the environment.

The invention relates to a method for preparing oudemansiella radicata nutrient powder by an oudemansiella radicata fermentation method. The method is characterized by comprising the following steps:(1) screening and activating oudemansiella radicata slant strains; (2) preparing a primary seed liquid; (3) preparing a secondary seed liquid; (4) preparing a tertiary seed liquid; (5) performing culturing in a fermentation tank; (6) drying oudemansiella radicata fermentation liquor; and (7) preparing oudemansiella radicata nutrient powder. The method has a scientific and reasonable design, fermentation is used by a special culture medium, and a fermentation mixture is directly dried for preparing powder. The process is simple and feasible, has good economic benefit, and can realize industrialproduction.

The invention discloses applications of siraitia grosvenorii vine in oudemansiella radicata cultivation. According to the applications, siraitia grosvenorii vine is dried in the sun, and is smashed into chippings so as to obtain a carbon source to prepare a cultivation matrix used for oudemansiella radicata cultivation. It is confirmed by experiment that the yield and quality of oudemansiella radicata cultured with the cultivation matrix are equal to those of oudemansiella radicata cultured with a conventional cultivation matrix prepared by taking cottonseed hull as a carbon source. Siraitia grosvenorii vine is widely available, is cheap, and can be used for replacing expensive cottonseed hull to reduce cultivation matrix cost and increase economic benefit; and utilization of waste products is realized.

The invention discloses a soil covering device for three-dimensional shelf cultivation of oudemansiella radicata. The soil covering device for three-dimensional shelf cultivation of the oudemansiella radicata comprises a base, wherein four moving devices are arranged on the lower end face of the base, four stand columns which are symmetrically arranged are fixedly connected to one side wall, away from the moving devices, of the base, and containing grooves are formed in one side walls, away from the base, of the four stand columns; working pipes are fixedly connected to the inner walls of one sides, close to the base, of the four containing grooves, a plurality of sliding openings formed at equal intervals are formed in the inner walls of the four containing grooves correspondingly, protruding blocks are slidably connected into the multiple sliding openings, working springs are fixedly connected to one side walls, close to the working pipes, of the protruding blocks correspondingly, and one ends, away from the protruding blocks, of the multiple working springs are fixedly connected to the outer walls of the four working pipes correspondingly. According to the soil covering device for three-dimensional shelf cultivation of the oudemansiella radicata, a user can conveniently, quickly and safely complete the soil covering work of the oudemansiella radicata on shelfs with different heights, so that great convenience is brought to the user to use the device.

The invention discloses a method for culturing oudemansiella radicata through eutrophic artificially soil. The eutrophic artificially soil is mainly prepared by taking mixed saw dust and bagasse as main materials and taking cellobiose, yeast extract, refined building materials and an adhesion agent as auxiliary materials and is fluffy in soil texture and good in air permeability, the demands of oudemansiella radicata for a carbon source and a nitrogen source are met, waste is utilized, and the artificially- soil which is suitable for growth of oudemansiella radicata and rich in nutrient can also be provided; in addition, the artificially soil is prepared into a specific granular shape, and the situation is reduced that when ripe oudemansiella radicata is harvested, a large amount of soil is attached to the roots of oudemansiella radicata, and accordingly the roots are cut off; the cost is lowered, and good-quality oudemansiella radicata is obtained.

The invention discloses application of sophora flower bud residues and kudzu vine root residues to a cultivation material for Oudemansiella radicata. According to the invention, the sophora flower budresidues are applied as a nitrogen source and the kudzu vine root residues are applied as a carbon source to the cultivation material for Oudemansiella radicata. The novel cultivation material is provided for development of Oudemansiella radicata, and the cultivation material is low in cost and high in nitrogen content.

The invention discloses an oudemansiella radicata cultivation formula, a preparation method and a cultivation method. The oudemansiella radicata cultivation formula is characterized by comprising thefollowing components including, by weight, 35%-55% of corn stalks, 15%-30% of cottonseed hulls, 8%-24% of corncob and 2%-12% of soybean meal, and the pH value is adjusted to 6.2-6.9. In order to overcome the defects in the prior art, the oudemansiella radicata cultivation formula, the preparation method and the cultivation method have the advantages that the corn stalks are taken as a main formula, waste is turned into wealth, and the corn stalks are combined with other components according to a proper ratio, so that the growth speed of the oudemansiella radicata hyphae can be remarkably increased, the biomass of the oudemansiella radicata hyphae is increased, and the yield of oudemansiella radicata is effectively increased.

The invention provides an oudemansiella radicata SSR molecular marker primer group and application thereof. According to the invention, 20 pairs of SSR labeled primers with strong specificity, good stability and high polymorphism are obtained by screening based on a whole genome sequence of oudemansiella radicata as a template, and the nucleotide sequences of the primers are shown as SEQ ID NO: 1-SEQ ID NO: 40. The oudemansiella radicata SSR molecular marker primer group can be used for identifying an oudemansiella radicata strain and distinguishing the oudemansiella radicata strain from other non-oudemansiella radicata strains, and can also be used for carrying out traceability detection on the oudemansiella radicata strain. Compared with conventional morphological detection and the like, the method has the advantages of being easy to operate, good in repeatability, stable and accurate in result and the like, and has important reference value and guiding significance for collybia radicata variety identification, genetic diversity analysis, phylogenetic analysis or polymorphism analysis and the like.

The invention provides a method for identifying fruiting phenotype of Ooospore oudemansiella radicata based on efficient capillary electrophoresis, the method comprises the following steps: carrying out capillary electrophoresis on a sample solution under the electrophoresis conditions that the effective detection length of an uncoated quartz capillary is 48-60cm; the detection wavelength is 190-225 nm; the separation voltage ranges from 25-30 kV; the temperature is 20-25 DEG C; the pressure is 0.5-0.8 psi, the sample introduction is performed for 3-5 seconds, and a running buffer solution is a borax buffer solution with the concentration of 25-50 mmol / L and a sodium citrate buffer solution with the concentration of 20-25 mmol / L; the operation time is 30-35 minutes, and obtaining the fingerprint spectrum. According to the method for identifying the fruiting phenotype of the Ooospora radicata based on efficient capillary electrophoresis, a W-shaped triple difference peak serves as a key indication peak for identifying the fruiting phenotype of different sporocarp of the Ooospora radicata, and a new method is provided for screening and identifying fruiting strains after wild domestication and cross breeding of the Ooospora radicata in the later period.

The invention discloses a method for preparing an edible fungus protein extract with antitumor efficacy. The preparation method comprises the following steps of: 1) extracting by water after crushing edible fungus sporocarp, collecting a water-soluble matter to obtain an edible fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the edible fungus water-soluble extract, collecting sediments, and obtaining the edible fungus protein extract with the antitumor efficacy after dialyzing the sediments by deionized water, wherein the edible fungus is at least one of the following funguses: auricularia polytricha, pleurotus citrinopileatus, abalone mushroom, pycnoporus cin, cordyceps militaris, pleurotus djamor, black fungus, clitocybe maxima, flammulina velutipes, oudemansiella radicata and pholiota adipose. The edible fungus protein extract disclosed by the invention has a significant proliferation inhibition role on an HepG2 cell, an MCF-7 cell and an A-549 cell, and can be used for preparing a product for resisting tumors or tumor cells.

The invention discloses an Oudemansiella radicata cultivation material prepared by using Flos sophorae residue and mulberry branch, wherein the raw materials comprise, by weight, 9-11 parts of Flos sophorae residue, 75-80 parts of mulberry branch, 1 part of gypsum powder, and 1 part of calcium superphosphate. According to the present invention, the new carbon source and nitrogen source materials with characteristics of low price and high quality are provided for the cultivation of Oudemansiella radicata so as to reduce the Oudemansiella radicata production cost and improve the yield and the quality, and the influence that Flos sophorae residue and mulberry branch are not treated in time to pollute the environment is solved.

The invention discloses application of sophora flower bud residues and grape vines to a cultivation material for Oudemansiella radicata. According to the invention, the sophora flower bud residues areapplied as a nitrogen source and the grape vines are applied as a carbon source to the cultivation material for Oudemansiella radicata. The novel cultivation material is provided for development of Oudemansiella radicata, and the cultivation material is low in cost and high in nitrogen content and is an innovative and creative cultivation material for Oudemansiella radicata.

The invention relates to an Oudemansiella radicata culture material using the root of sprouted beans and corn cob as carbon sources, wherein the Oudemansiella radicata culture material is prepared from the following components by weight: 40-45 parts of corn cob debris, 40-45 parts of the root of sprouted beans, 13-16 parts of bran, 0.8-1.2 parts of sugar and 0.8-1.2 parts of calcium carbonate through the existing technology. According to the present invention, the yield and the quality of the Oudemansiella radicata cultivated by using the crop waste as the carbon source are almost the same as the yield and the quality of the Oudemansiella radicata cultivated by using sawdust and cottonseed hull as the carbon source while the production cost is substantially reduced.

The invention is suitable for the technical field of culture of oudemansiella radicata, and provides an oudemansiella radicata culture medium as well as a preparation method and application thereof. The culture medium is prepared from sawdust, corncobs, yeast powder, sugar, wheat bran, monopotassium phosphate and water, the culture medium is heated, mixed, stirred and fermented to obtain a filtrate as a culture medium, and then, the culture medium is subjected to sterilization, inoculation and culture to obtain mature hyphae. The culture medium adopts a simple formula and is suitable for large-scale production, the source of raw materials is convenient, and the liquid strain culture process is simple, easy to operate and suitable for large-scale production, can also avoid forest and bacteria contradictions, and is conducive to sustainable development.