Compound oudemansiella radicata polysaccharide oral liquid and preparation method and application thereof
A technology of Rhizopus rhizogenes and oral liquid, which is applied in the directions of pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems such as the preparation method of Rhizopus rhizome polysaccharide oral liquid that has not been reported in the literature and the like. , to achieve the effect of improving the level of hemolysin, high-efficiency scavenging ability, and solving the high requirements of the growth environment
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Embodiment 1
[0043] A kind of compound rhizome polysaccharide oral liquid, it is made up of the component of following mass percentage:
[0044]
[0045] The rest is water.
[0046] The preparation method is as follows:
[0047] (1) Seed culture: cutting the preserved Rhizome slant bacterial classification (PDA medium) to get the soybean size bacterium, inoculated in the liquid seed medium (corn flour 3.0%, sucrose 3.0%, peptone 0.5%, K 2 HPO 4 0.5%, MgSO 4 ·7H 2 O 0.2%), cultured at 25°C, 180 rpm for 5 days.
[0048] (2) Fermentation culture: the liquid seeds obtained are inoculated in the fermentation medium according to the inoculum size 10% (bean cake 3.0%, corn flour 1.0%, sucrose 2%, K 2 HPO 4 1.0%, MgSO 4 ·7H 2 O 0.3%, MnSO 4 ·H 2 O 0.4%, CaCO 3 0.2%), cultured at 25°C and 200 rpm for 7 days.
[0049] (3) Cell microfiltration: ceramic membrane microfiltration machine filters the fermentation broth to obtain mycelium filter cake and extracellular polysaccharide filtr...
Embodiment 2~10
[0080] Examples 2 to 10 respectively used the proportioning raw materials as described in Table 6 to prepare the compound rhizome polysaccharide oral liquid, wherein each proportioning ratio is a mass percentage ratio, and other steps are the same as in Example 1.
[0081] Table 6. Raw material ratio of the compound rhizome polysaccharide oral liquid of embodiments 2-10
[0082]
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