Microbial fermentation antibiotic-free feed and preparing method thereof

A microbial fermentation, antibiotic-free technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limited effect, inconvenient addition of multivitamins, insufficient addition, etc.

Inactive Publication Date: 2010-09-15
湖南创新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The types of beneficial bacteria are limited to anaerobic bacteria and facultative anaerobic bacteria, and the effect of replacing antibiotics is limited: Fermentation using a fermentation bag with a breathing valve can only cultivate anaerobic bacteria and facultative anaerobic bacteria, such as Bacillus and A variety of enzyme-producing aerobic bacteria cannot grow and reproduce, let alone metabolites, and the role of anaerobic bacteria and facultative anaerobic bacteria in replacing antibiotics is limited;
[0006] 2. Fermentation of all components of the feed without sterilization leads to uncontrollable quality: it is recognized worldwide that it is extremely dangerous to use raw material fermentation. Since the amount of bacteria varies with raw materials, it is beneficial to use fermentation bags with breathing valves for fermentation. At the same time, the miscellaneous bacteria brought by the raw materials are also cultivated. It is not ruled out that they contain pathogenic bacteria. Once an epidemic occurs, it will cause huge losses;
[0007] 3. Energy loss and vitamin problems: All the components of the feed are fermented as the medium. Due to the generation of fermentation heat, a large amount of energy components are lost; no multivitamins are added to the feed formula, mainly due to the limitation of its process conditions, which is inconvenient. Add multivitamins, because multivitamins are fermented for dozens of days in an environment with a water content of 40% to 50%, a temperature of 30°C to 40°C, and a pH value of ≤5, plus the destruction of minerals, warehouse storage, transportation, and breeding Stored in households, the potency of vitamins is very small, if no multi-vitamins are added or feeds with insufficient amounts are added, animals will have serious consequences of various vitamin deficiencies after eating;
[0008] 4. High cost and inconvenient use: the moisture in the feed is as high as 40%, and the fermentation of the fermentation bag with a breathing valve increases the cost of packaging and transportation, and it is prone to damage, mildew and waste during transportation and storage. , It is also easy to form sticky and wet lumps that are difficult to break, so that the farmers increase the mixing equipment and still mix unevenly, which is extremely inconvenient to use;
[0009] 5. Long turnover period and large storage capacity: the inoculated bacteria in fermentation production only account for one ten thousandth of the fermentation material (that is, one ten thousandth of the inoculation amount), resulting in a fermentation cycle of dozens of days, making the manufacturer The amount of press storage is extremely large, which increases the production cost

Method used

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  • Microbial fermentation antibiotic-free feed and preparing method thereof
  • Microbial fermentation antibiotic-free feed and preparing method thereof
  • Microbial fermentation antibiotic-free feed and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0064] A microbial fermented antibiotic-free feed, which includes a compound material and a compound fermented material; the compound material is composed of raw materials in the following proportions by weight: 30-50 parts of corn (for example: 30 parts, 40 parts, 50 parts), soybean meal 20-50 parts 40 parts (for example: 20 parts, 30 parts, 40 parts), 5-20 parts of cottonseed meal (for example: 5 parts, 12 parts, 20 parts), 2-5 parts of calcium hydrogen phosphate (for example: 2 parts, 3 parts, 5 parts), salt 0.2~0.4 parts (example: 0.2 parts, 0.3 parts, 0.4 parts), compound trace elements 0.1~0.2 parts (example: 0.1 parts, 0.2 parts), multivitamins 0.2~0.4 parts (example: 0.2 parts , 0.3, 0.4);

[0065] The composite fermented material is composed of fermented material in the following proportions by weight: 30 to 70 parts of Lactobacillus acidophilus fermented material (for example: 30 parts, 45 parts, 70 parts), 10 to 20 parts of Bacillus subtilis fermented material (for ...

Embodiment 2

[0081] A preparation method for microbial fermentation antibiotic-free feed, comprising the steps of:

[0082] 1. Preparation of bacterial strains: Lactobacillus acidophilus, Bacillus subtilis, Saccharomyces cerevisiae, Trichoderma longifolia, Aspergillus niger, and Aspergillus oryzae were taken as the starting bacteria, and the temperature was gradually increased for acclimation, extraction, and rejuvenation, respectively, to obtain strains capable of achieving resistance to strains. The target strains at the grain temperature, and then expanded and cultured separately to obtain a bacterial content of 1×10 7 ~1×10 8 Each bacterial strain liquid per g, that is, Lactobacillus acidophilus seed liquid, Bacillus subtilis seed liquid, Saccharomyces cerevisiae seed liquid, long handle Trichoderma seed liquid, Aspergillus niger seed liquid, Aspergillus oryzae seed liquid.

[0083] The above strains can be purchased from the China Industrial Microbiology Culture Collection Management...

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Abstract

The invention discloses a microbial fermentation antibiotic-free feed, which comprises a batch and a composite fermentation material. The batch consists of the following raw materials in part by weight: 30 to 50 parts of corn, 20 to 40 parts of bean pulp, 5 to 20 parts of cotton seed meal, 2 to 5 parts of calcium hydrophosphate, 0.2 to 0.4 part of salt, 0.1 to 0.2 part of composite trace element, and 0.2 to 0.4 part of composite vitamin; and the composite fermentation material consists of the following fermentation materials in part by weight: 30 to 70 parts of lactobacillus acidophilus fermentation material, 10 to 20 parts of bacillus subtilis fermentation material, 20 to 35 parts of saccharomyces cerevisiae fermentation material, 1 to 3 parts of trichoderma longbrachiatum fermentation material, 1 to 3 parts of aspergillus niger fermentation material, and 1 to 3 parts of aspergillus oryzae fermentation material, wherein the weight ratio of the batch to the composite fermentation material is 96-92:4-8.

Description

technical field [0001] The invention relates to an animal feed, in particular to a microbial fermented antibiotic-free feed and a preparation method thereof. Background technique [0002] The addition of antibiotics to feed in the 20th century has brought about the rapid development of the global animal husbandry industry. However, long-term feeding of animals with antibiotic-added feed will produce drug-resistant strains in the body and cause problems such as drug residues in animal products. With the gradual improvement of people's living standards, consumers' demand for livestock and poultry products has transitioned from the pursuit of quantity to the pursuit of quality, so the practice of adding antibiotics to feed is becoming more and more restricted. Currently, in Europe, North America, Asia and other regions Many countries have banned the addition of antibiotics to feed. [0003] Developing antibiotic-free feed and solving the problem of drug residues from the sour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16A23K1/175C12N1/20C12N1/14C12N1/18C12R1/23C12R1/125C12R1/865C12R1/885C12R1/685C12R1/69
Inventor 王德昌李清定彭立秋袁钟宇
Owner 湖南创新生物科技有限公司
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