Microbial fermentation antibiotic-free feed and preparing method thereof
A microbial fermentation, antibiotic-free technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limited effect, inconvenient addition of multivitamins, insufficient addition, etc.
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Embodiment 1
[0064] A microbial fermented antibiotic-free feed, which includes a compound material and a compound fermented material; the compound material is composed of raw materials in the following proportions by weight: 30-50 parts of corn (for example: 30 parts, 40 parts, 50 parts), soybean meal 20-50 parts 40 parts (for example: 20 parts, 30 parts, 40 parts), 5-20 parts of cottonseed meal (for example: 5 parts, 12 parts, 20 parts), 2-5 parts of calcium hydrogen phosphate (for example: 2 parts, 3 parts, 5 parts), salt 0.2~0.4 parts (example: 0.2 parts, 0.3 parts, 0.4 parts), compound trace elements 0.1~0.2 parts (example: 0.1 parts, 0.2 parts), multivitamins 0.2~0.4 parts (example: 0.2 parts , 0.3, 0.4);
[0065] The composite fermented material is composed of fermented material in the following proportions by weight: 30 to 70 parts of Lactobacillus acidophilus fermented material (for example: 30 parts, 45 parts, 70 parts), 10 to 20 parts of Bacillus subtilis fermented material (for ...
Embodiment 2
[0081] A preparation method for microbial fermentation antibiotic-free feed, comprising the steps of:
[0082] 1. Preparation of bacterial strains: Lactobacillus acidophilus, Bacillus subtilis, Saccharomyces cerevisiae, Trichoderma longifolia, Aspergillus niger, and Aspergillus oryzae were taken as the starting bacteria, and the temperature was gradually increased for acclimation, extraction, and rejuvenation, respectively, to obtain strains capable of achieving resistance to strains. The target strains at the grain temperature, and then expanded and cultured separately to obtain a bacterial content of 1×10 7 ~1×10 8 Each bacterial strain liquid per g, that is, Lactobacillus acidophilus seed liquid, Bacillus subtilis seed liquid, Saccharomyces cerevisiae seed liquid, long handle Trichoderma seed liquid, Aspergillus niger seed liquid, Aspergillus oryzae seed liquid.
[0083] The above strains can be purchased from the China Industrial Microbiology Culture Collection Management...
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