539 results about "Antibiotic free" patented technology

The invention relates to an antibiotic-free piglet conservation material and a preparation method thereof. The conservation material comprises the following raw materials of puffed corn, wheat, a base material, efficient cream, soybean meal, fermentation soybean meal, whey powder, composite yeast, rice protein powder, stone powder, calcium hydrogen phosphate, lysine, methionine, threonine, choline chloride, liquid phytase, liquid xylanase, a acidifying agent, sodium butyrate and a premix compound. The antibiotic-free piglet conservation material can increase immunity of the piglet, prevents the piglet from generating drug resistance and appearing drug residue problems, maintains stability of intestinal flora, and improves digestion and absorption for forage of the piglet.

A host-vector system that uses the RNA-based copy number control mechanism of ColE1-type plasmids for regulating the expression of a marker gene allows for antibiotic-free selection of plasmids and is useful for production of plasmid DNA and recombinant proteins.

Improved DNA vaccine plasmids are disclosed that contain novel immunostimulatory RNA compositions. The improved plasmids eliminate all extraneous sequences, incorporate a novel antibiotic free short RNA based selectable marker, increase eukaryotic expression using a novel chimeric promoter, improve yield and stability during bacterial production, and improve immunostimulation. These vectors are utilized in immunization to elicit improved immune responses or therapy to induce type 1 interferon production.

The invention relates to a Chinese herbal medicine feed additive for enhancing chicken immunity, a preparation method and a feed. The additive includes ingredients of, by weight, 30-90 parts of radix astragali, 30-90 parts of radix codonopsis, 30-90 parts of Chinese yam, 30-90 parts of poria cocos, 30-90 parts of coix seeds, 15-45 parts of fried lotus seed, 10-30 parts of bighead atractylodes rhizome, 10-30 parts of rhizoma atractylodis, 10-30 parts of dried orange peel, 15-45 parts of epimedium, 30-90 parts of stir-baked medicated leaven, 30-90 parts of stir-baked malt, 10-30 parts of betel nut, 10-30 parts of rhizome of cyrtomium and 10-30 parts of licorice. According to a compatibility principle of traditional Chinese medicine and a modern pharmacological research, the invention uses amedicament comprised of natural Chinese herbal medicines as an additive to feed chickens, so as to enhance chicken immunity, reduce infectious diseases, pathogenic microorganisms and parasitic diseases; besides, the additive has no antibiotic residuals, so as to enhance chicken and egg quality.

The invention discloses an antibiotic-free feed for nursery pigs and a preparation method thereof. Corn, barley and soybean meal are pre-cleaned to remove impurities and enter a permanent magnet drum to remove tramp iron; the materials enter a bin for smashing respectively; smashing is performed, 100% of smashed materials are subjected to 14-mesh sieving, and 95% of the smashed materials are subjected to 20-mesh sieving; suckling pig compound enzyme, a vitamin premixing agent, a microbial ecological agent, plant essential oil, phagostimulant, high temperature resistant phytase, an organic microelement premixing agent, a Chinese herbal medicine prescription, yeast cell wall polysaccharide, glycerol monobutyralte and an emulsifier are firstly mixed fully for use; then the corn, the barley, the soybean meal, soybean oil, extruded soybeans, fermented soybean meal, import fish meal, whey mist, salt, calcium formate, calcium hydrophosphate, amino acid, composite organic acid, an antioxidant and a mildewproof agent are weighed in proportion and then are compounded and mixed; finally granulation is performed. An antibiosis growth promotion effect which is the same as or better than that obtained by using antibiotics can be achieved, the sensibility of germs to the antibiotics is improved, and the treatment drug effect is improved.

The invention discloses an antibiotic-free pig creep conservation general concentrate, which comprises the following components by weight percent: 0.03 to 0.15% of vitamins, 0.5 to 2.5% of microelements, 0.01 to 1.8% of amino acid, 0.2 to 2.5% of prebiotic, 0.03 to 0.15% of enzymic preparation, 0.2 to 5.0% of lactic acid bacteria, 5 to 40% of fermented soybean meal, 10 to 40% of whey powder, 4 to 16% of glucose powder, 10 to 40% of protein supplement, and 2 to 10% of vegetable oil. The antibiotic-free pig creep conservation general concentrate provided by the invention can be used for well controlling the diarrhea of piglets, and solving the problems of low feed intake, slow growth, and low immunity of piglets and the like, and has good palatability and high feed conversion rate.

The invention relates to an ecological cultivation method with health care fermented feed matched with a probiotic fermentation bed. The method includes the steps that first, probiotic fermentation is carried out, wherein the probiotic fermentation comprises anaerobic fermentation of anaerobes and aerobic fermentation of aerobic microorganisms; second, the probiotic fermentation bed is prepared, wherein various raw materials for preparing the material of the fermentation bed are evenly mixed so as to obtain organic bedding, bacterium solution is inoculated for fermentation, and the organic bedding contains saw dust, rice husks, bran and first compound Chinese herbal medicine; third, the fermented feed contains corn, bean pulp, vitamins, microelements, mineral substances, amino acids, other additives, second compound Chinese herbal medicine and the like, and microorganisms are added into the feed components for fermentation. The ecological cultivation method has the advantages that water can be purified, and hidden danger to health of hogs caused by a water source is blocked; the using amount of the microelements is reduced, resources are saved, emissions are reduced, and the environment is protected; no antibiotics or residue exists, and food safety and human health are guaranteed; the Chinese herbal medicines have the functions of being resistant to disease and promoting growth and can replace antibiotics.

The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.

The invention discloses a feed special for a low-fat meat chicken without antibiotic, which consists of the following by weight percentage: 10-25 of lactobacillus fermented material and 75-90 of main material, wherein the lactobacillus fermented material consists of the following raw materials: cotton dregs, rapeseed dregs, bran, peanut meal, maize and lactobacillus fermented liquid; and the main material consists of the following raw materials: bean dregs, maize protein powder, maize flour stone dust, calcium hydrophosphate, orexin and growth promotion agents, flavoring agents and trace elements. 10-25 percent of the obtained lactobacillus fermented material is taken according to the percentage of the total weight of the feed for bagging, and 75-90 percent of the obtained main material is taken according to the percentage of the total weight of the feed for packing after bagging, thus the feed special for the meat chicken is prepared. The chicken fed by the feed which is prepared by uniformly mixing the lactobacillus fermented material and the main material have pork type meat quality, abundant muscles, less fat and high protein content, are inspected to have no chloromycetin, terramycin, aureomycin and sulfanilamide medicaments by the national meat product supervision and inspection center and completely reach a non-antibiotic standard and meet the requirement of food security of the European Union.

The invention provides an antibiotic-free mixed feedstuff and a preparation method thereof. The invention belongs to the field of animal feedstuffs. According to the invention, a mixed feedstuff containing Chinese herbal medicines is mixed with a mixed fermentation material containing various probiotics according to a certain proportion, such that the feedstuff provided by the invention is obtained. The mixed feedstuff containing Chinese herbal medicines comprises half-dried ginkgo leaves or ginkgo leaf extraction residues, Japanese honeysuckle stems, balloon flower root, soybean meal, peanutmeal, corn powder, wheat flour, calcium hydrogen phosphate, common salt, composite microelement, mulvital, and selenium-enriched yeast. The mixed fermentation material containing various probiotics comprises an enterococcus faecalis fermentation material, a bacillus licheniformis fermentation material, a saacharomyces cereisiae fermentation material, a Trichoderma reesei fermentation material, and an aspergillus niger fermentation material, which are mixed according to a certain proportion. Microbe strains are processed through targeted acclimatization, and are cultivated by using a solid fermentation medium, such that the mixed fermentation material is obtained. The antibiotic-free mixed feedstuff provided by the invention assists in promoting growth, and brings no toxic or side-effect. The feedstuff causes no antibiotic residue, no pollution, and no drug resistance. With the mixed fermentation the utilization rate of the feedstuff can be improved, healthy growth of live stocks can be promoted, and meat qualities of the live stocks can be improved.

The invention discloses an antibiotic free, hormone free and harmful additive free biological mixed feed capable of improving quality of meat and suitable for feeding of various meat animals, and a production method thereof. The biological mixed feed comprises the following materials, by weight: 8-10% of biological fermentation cotton seed meal, 3-5% of polypeptide biological blood powder, 80-84% of mixed forage powder, 3-5% of feed additive and 1-5% of plant oil. According to exogenous enzyme and microbial fermentation, macro-molecule protein raw materials are decomposed into polypeptides and small peptides, so as to degrade anti-nutritional factors like non-starch polysaccharides in the forage and increase utilization rate of the forage; meanwhile, abundant B-group vitamin, free amino acid and organic acidity are generated during a fermentation process to promote deposition of flavor amino acid, inosinic acid and unsaturated fatty acid in muscle, so as to enhance quality of meat. Freshwater fish, fryers and growing and fattening pigs fed by the biological mixed feed for animals of the invention have characteristics of fast growth speed, and elastic, chewy, delicious and strongly fragrant fish meat, chicken and pork. The method of the invention is simple, requires no special equipment and is suitable for industrialized production.

The present invention provides a microbial fermented composite antibiotic-free feed containing natural antibacterial components. The feed comprises a fermented product based on the united segmented fermentation of aerobic-anaerobic composite strains, composite natural antibacterial components, a non-plant source protein/polypeptide compound nutrient supplement agent, and an additive comprising atleast a soybean protein amino acid/polypeptide compound chelate. The composite strains comprises at least lactic acid bacteria, bacillus subtilis, yeast selected from candida utilis or saccharomyces cerevisiae, and enterococcus faecalis. The fermented product based on the united segmented fermentation is prepared by carrying out secondary fermentation on fermentation raw materials through the aerobic-anaerobic composite strains. The combination of the microbial fermented feed and the natural antibacterial components is beneficial to the balance of intestinal flora of animals, and can prevent the dysbacteriosis of digestive tracts, improve the immunity functions of animal organisms, reduce or replace the use of feed antibiotics, and significantly improve the digestion and absorption efficiency of the feed and the meat quality of animals.

The invention discloses an efficient environment-friendly antibiotic-free compound feed for piglets. In parts by weight, the efficient environment-friendly antibiotic-free compound feed for piglets comprises 20-70 parts of corns, 10-70 parts of sorghum, 10-22 parts of bean pulp, 3-10 parts of DDGS, 0-15 parts of soybean oil, 0-10 parts of rice bran, 0-8 parts of bran, 2 parts of multi-vitamin multi-mineral substance additive premix, 0.1-0.3 part of an acidifying agent and 0.1-0.3 part of a piglet feed antibiotic-free additive. The efficient environment-friendly antibiotic-free compound feed is free of any growth promoting antibiotic and has the advantages of improving the feed digestibility, improving the piglet growth performance, improving the swinery immunity level and decreasing the diarrhea incidence rate.

The invention discloses piglet early-stage microbial fermented antibiotic-free feed, which is mixed by a batch material and a complex ferment material, wherein the weight ratio of the batch material and the complex ferment material is 92-96:4-8; the complex ferment material is mixed by the following ferment materials by weight: 3-6 parts of lactobacillus acidophilus ferment materials, 0.1-0.5 parts of bacillus subtilis ferment materials, 0.35-1.07 parts of saccharomyces cerevisiae ferment materials, 0.05-0.10 parts of aspergillus niger ferment materials, and 0.05-0.33 parts of aspergillus oryzae ferment materials. The piglet early-stage microbial fermented antibiotic-free feed obtained by the invention contains various beneficial bacteria with a large quantity, also contains various high-activity digestive enzymes such as beta-glucanase, xylanase, cellulase, and protease, has very good synergistic effect, can increase body immunity of animals, improve intestinal tract micro-ecologicalenvironment, thus improve animal health status, increase the feed digestion utilization rate, and improve the culture economic benefit.

The invention discloses an automatic production device of an environmental protection antibiotic-free fermented?feed. The automatic production device comprises a cleaning system, a crushing system, a feed proportioning system, a microbe?solution injection system, a mixing system and a packaging system, wherein the discharge port of the cleaning system is connected with the feed port of the crushing system, the discharge port of the crushing system is connected with the feed port of the feed proportioning system, the discharge port of the feed proportioning system is connected with the solid-material feed port of the mixing system, the discharge port of the microbe solution injection system, the discharge port of the microbe solution injection system is connected with the microbe solution feed port f the mixing system, and the discharge port of the mixing system is connected with the packaging system. The automatic production device has advantages of convenient operation, safe and reliable use, environmental protection, and no pollution. The special process design concept?is adopted, the solid and solution mixture design is utilized, the feed is packaged and then fermented, the production package mode, artificial stacking?fermentation, of the traditional production is changed, and the computer automatic control way is adopted, so as to achieve perfect automatic production of the antibiotic-free fermented?feed.

The invention discloses normal-temperature yogurt and a preparation method thereof. According to the normal-temperature yogurt, the viscosity is 200-250cp, the total protein content by mass percent is2.56%-3.03%, the fat content by mass percent is 3.5%-6.0%, the carbohydrate content by mass percent is 4.76%-12.14%, and the acidity is 70-90 degrees T. The preparation method of the yogurt comprisesthe following steps: uniformly mixing 81.491%-93.327% of pre-sterilized antibiotic-free milk, 4%-8% of a sweetening agent, 2.25%-8.61% of light cream and 0.42%-0.84% of a stabilizing agent by a vacuum mixing mode, homogenizing the mixed liquid, and performing pasteurization on the homogenized liquid, wherein the percentages are the mass percentages of the components in the mixed liquid; cooling the sterilized mixed liquid, adding 0.003%-0.009% of a starter and 0%-0.1% of essence, performing fermenting, wherein the percentage is the mass percentage of the starter in the mixed liquid, and performing emulsion breaking when the acidity reaches 70-90 degrees T to obtain a yogurt base material; and performing homogenization and pasteurization on the yogurt base material for the second time, wherein the sterilization temperature is 70-80 DEG C and the sterilization time is 10-40s, performing cooling to 20-30 DEG C after sterilization and performing nitrogen-filled filling. The product is high in fat content, suitable for young people to drink and smooth and delicate in mouthfeel.

The invention discloses an antibiotic-free conservation fodder for piglets. The conservation fodder comprises the following components in part by weight: 550-640 parts of maize, 40-60 parts of super steam fish meal, 200-250 parts of peeled soybean meal, 40-60 parts of whey powder, 20-30 parts of glucose, 1-2 parts of table salt, 5-9 parts of secondary calcium phosphate, 10-25 parts of Nengbao, 7-12 parts of calcium formate, 10 parts of premix of the conservation fodder for piglets, 6-11 parts of lysine, 1 part of acidifying agent (coated), premix of vitamins, premix of compound minor elements for piglets, phytase, multienzyme, antibacterial peptide, prebiotics, antioxidant and zeolite powder. The antibiotic-free conservation fodder for piglets does not contain any antibiotics, has no defect of drug resistance of animals, drug residues, environmental pollution and the like, and can rival with or even exceed the antibiotic-contained piglet conservation fodder in the prior art in controlling diarrhea and promoting growth of the piglets.

The invention discloses a low-phosphorus antibiotic-medicine-free complete feed for fattening pigs. The feed is prepared from a trace element premix, a vitamin premix, a mineral premix, compound enzyme, a feed attractant and a feed toxin adsorbent. According to a formula, the feed comprises the following components in percentage by weight: 60.00 percent of corn, 15.00 percent of 46 percent bean pulp, 5.00 percent of low-toxicity rapeseed meal, 5.00 percent of wheat bran, 2.00 percent of soybean phospholipid oil, 3.00 percent of garlic powder, 3.00 percent of pepper meal, 2.10 percent of lees powder, 2.00 percent of molasses, 1.05 percent of mountain flour, 0.75 percent of calcium hydrophosphate, 1.00 percent of low-phosphorus antibiotic-medicine-free core material premix special for fattening pigs, and 0.10 percent of feed mould inhibitor. The invention is characterized in that the feed is high-lysine, low-phosphorus and antibiotic-medicine-free, has comprehensive nutrition, is high in palatability and can promote the growth of the pigs; the daily gain of growing pigs can reach 706g/day; and the feed conversion rate is less than 3.02:1.

The invention discloses production method for a recombinant human granulocyte colony-stimulating factor. According to the invention, an engineered strain of an escherichia coli expressed recombinant human granulocyte colony-stimulating factor is cultured to obtain an inclusion body, the engineered strain of the escherichia coli expressed recombinant human granulocyte colony-stimulating factor is pKG931/HB101, a culture method comprises multiplication culture and culture in a fermentation cylinder, an antibiotic-free medium containing yeast and peptone is employed in both culture, a high pressure homogenizer is used for breaking of bacteria, and denaturation, renaturation and chromatography are carried out so as to obtain a high purity G-CSF stock solution. The production method provided by the invention has the advantages of a short production period, high production efficiency, a great production scale, especial suitability for industrial production and reduction in production cost.

The invention discloses an antibiotic-free feed capable of increasing food consumption of weaned piglets. The antibiotic-free feed comprises the following components in parts by weight: 200 parts of corn, 150 parts of puffed corn, 20 parts of broken rice, 30 parts of puffed broken rice, 90 parts of soy protein concentrate, 45 parts of puffed soybeans, 40 parts of fermented soybean meal, 30 parts of soybean meal, 50 parts of fish meal, 100 parts of whey powder, 30 parts of whole milk powder, 10 parts of yeast hydrolyate, 50 parts of coconut oil, 10 parts of soybean oil, 90 parts of carbohydrate, 4 parts of stone powder, 3 parts of calcium dihydrogen phosphate, 1 part of choline chloride, 8 parts of amino acids, 5 parts of glutamine, 0.5 part of composite vitamins, 10 parts of composite trace elements, 3 parts of a composite acidulant, 15 parts of lactic acid, 0.5 part of a compound enzyme preparation, 1 part of prebiotics and 5 parts of a Chinese herbal medicine preparation. The feed obviously increases the growth properties and the health level of piglets a week after weaning.

The invention discloses an antibiotic-free weaned-piglet complete feed and a preparing method thereof. The antibiotic-free weaned-piglet complete feed is antibiotic-free efficient complete feed capable of meeting the nutritional requirements of weaned piglets, promoting digestion and using of nutrient substances, improving the immunity function and the resistance of piglet bodies and reducing the diarrhea rate. The preparing method includes the steps that in the formula proportion, weighed corn, weighed high protein bean pulp and weighed extruded soybeans are smashed to be subjected to first-time high-temperature pelletizing, thin smashing is carried out after pellets cool, then weighed imported fish meal, weighed calcium hydrophosphate, weighed mountain flour, weighed salt, weighed soybean oil, weighed whey powder, weighed feed grade lysine, weighed methionine, weighed threonine, weighed premixed materials for the weaned piglets and compounded Chinese herbal medicine are mixed to be even, then second-time low-temperature pelletizing is carried out, cooling is carried out, then a finished product is packed, and the weaned piglets can be directly fed. Particles of the weaned-piglet feed produced with the preparing method are good in filling power, low in hardness, good in water dissolving performance, high in nutritive value, good in palatability, sanitary and safe, the diarrhea rate is reduced, the feed use ratio of the weaned piglets is increased, the production performance of the weaned piglets is improved, and antibiotic harm is avoided.