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130 results about "Bullfrog" patented technology

Bullfrog is a common English language term to refer to large, aggressive frogs, regardless of species.

Three-dimensional bullfrog breeding system and precise feeding control method

The invention discloses a bullfrog three-dimensional breeding system and an accurate feeding control method, and the bullfrog three-dimensional breeding system is characterized in that the bullfrog three-dimensional breeding system comprises a modular breeding unit, a gradient water level adjusting device and an interlayer isolation water circulation system, the three-step type three-dimensional framework is divided into a bottom layer tadpole pond, a middle layer froglet net cage and a top layer adult frog breeding bin, a control module is installed on each layer, and anti-escape assemblies are installed on the middle layer froglet net cage and the top layer adult frog breeding bin; the gradient water level adjusting device comprises a bionic step and a water level sensor which are arranged and installed, and the water level gradient adjusting and behavior inducing functions are achieved through engineering design. The interlayer isolation water circulation system comprises a distributed sensor, a UV sterilization and biological membrane filtration dual purification assembly and an adjustable air curtain, the adjustable air curtain forms a directional barrier between different layers of the three-dimensional culture system by dynamically regulating and controlling airflow, and independent environmental parameters of each layer are maintained.
Owner:JINING RUNTANG ECOLOGICAL AGRI TECH CO LTD

Bullfrog skin-derived peptide LGPQR as well as purification method and application thereof

The invention discloses bullfrog skin-derived peptide LGPQR and a purification method and application thereof.The molecular weight of the bullfrog skin-derived peptide LGPQR is 570.34 Da, and the amino acid sequence of the bullfrog skin-derived peptide LGPQR is shown as SEQ ID NO.1. The bullfrog skin-derived peptide LGPQR is obtained by extracting bullfrog skin enzymatic hydrolysate through an enzymolysis means to construct a polypeptide library, then screening the polypeptide library through computer-aided screening, and then evaluating the screened peptide fragments so as to be optimal. Finally, the purified bullfrog skin source peptide LGPQR is obtained. According to the bullfrog skin-derived peptide LGPQR as well as the purification method and the application thereof, the bullfrog skin-derived peptide LGPQR has relatively good functions of reducing blood sugar and resisting oxidation, has application potential of type II diabetes medicines, and is safe and free of toxic and side effects. The amino acid sequence is short, the operability is high, and the social popularization and application value is good.
Owner:化学与精细化工广东省实验室潮州分中心

Bullfrog skin source peptide as well as purification method and application thereof

The invention discloses bullfrog skin-derived peptide and a purification method and application thereof.The molecular weight of the bullfrog skin-derived peptide is 1035.52 Da, and the amino acid sequence of the bullfrog skin-derived peptide is shown as SEQ ID NO.1. The bullfrog skin-derived peptide is extracted through an enzymolysis means to construct a polypeptide library, then the polypeptide library is screened through computer-aided screening, then screened peptide fragments are evaluated so as to be optimized, and the bullfrog skin-derived peptide is obtained. And finally obtaining the purified bullfrog skin source peptide. According to the bullfrog skin source peptide and the purification method and application thereof, the bullfrog skin source peptide has good blood sugar reducing and oxidation resisting functions, has the application potential of type II diabetes mellitus drugs, and is safe and free of toxic and side effects. The amino acid sequence is short, the operability is high, and the social popularization and application value is good.
Owner:化学与精细化工广东省实验室潮州分中心

Bullfrog biological behavior recognition optimization system based on deep learning

The invention discloses a bullfrog biological behavior recognition optimization system based on deep learning, and relates to the technical field of biological recognition. Performing frame decomposition processing on the image data of the bullfrogs to obtain image data frames, extracting first-class feature information and second-class feature information in the image data frames, training an image recognition model to construct moving tracks of different bullfrogs, obtaining theoretical active coefficients and correlation coefficients of the bullfrogs according to the moving tracks, and obtaining the theoretical active coefficients and the correlation coefficients of the bullfrogs according to the theoretical active coefficients and the correlation coefficients; optimizing the theoretical activity coefficient to obtain an actual activity coefficient, inputting the actual activity coefficient into a preset behavior prediction model, obtaining a predicted movement track of the bullfrog, and obtaining predicted bullfrog density of different activity areas according to the predicted movement track; the bullfrog activeness monitoring accuracy can be improved, and the bullfrog density prediction accuracy can be improved.
Owner:HUNAN UNIV +1

Bullfrog peeling and pulling device

The utility model discloses a bullfrog skinning and pulling device, and particularly relates to the bullfrog skinning field, the bullfrog skinning and pulling device comprises a support plate, one side of the top of the support plate is fixedly connected with a support frame, the other side of the top of the support plate is fixedly connected with a fixed plate, and the top of one end of the support frame is provided with a first electric push rod; the moving end of the first electric push rod penetrates through the supporting frame and is fixedly connected with a lifting plate, and a plurality of drag hooks are fixedly connected to the bottom of the lifting plate. Firstly, a second electric push rod is started to control the moving plate to move away from the bottom of the lifting plate, bullfrogs are conveniently placed on the top of the moving plate, a motor is started to control the supporting pressing plate to drive the fixing pressing plate to clamp and fix bullfrog meat, the clamping and fixing strength can be improved, and the bullfrogs are moved to the bottom of the lifting plate, so that the bullfrog meat can be clamped and fixed. The first electric push rod is started to control the lifting plate to drive the drag hook to hook bullfrog skin, the skinning and pulling effects can be achieved when bullfrog meat is pulled, the bullfrog meat is not prone to falling off, and the using effect is improved.
Owner:SHANTOU HENGXING AQUATIC PRODUCTS CO LTD

Bullfrog breeding barrel facilitating excrement discharge

The utility model discloses a bullfrog breeding barrel convenient to discharge excrement, which comprises a support, a barrel body arranged on the support, a water outlet arranged at the bottom of the barrel body and an adjusting support plate arranged in the barrel body, grid holes are arranged on the adjusting support plate, and the size of the grid holes can be adjusted through the adjusting support plate. A sewage containing cavity is formed by the adjusting supporting plate and the bottom of the barrel body, and impurities discharged into water are precipitated into the sewage containing cavity through the grid holes. The bullfrog breeding device can solve the problems that a pool cannot be cleaned and breeding water is troublesome to replace, bullfrogs are not prone to being damaged when water is replaced, antibiotic drugs are reduced or avoided, the growth environment is stable, the growth cycle is stable, breeding facilities are convenient to build, cost is low, and popularization is easy.
Owner:ZHEJIANG LIANXING FEED TECH

Packaging bag (American bullfrog and fish hot pot seasoning)

ActiveCN309307083SProcess engineeringBullfrog
1. Name of the Design Product: Packaging Bag (American Bullfrog and Fish Hot Pot Seasoning). 2. Use of the Design Product: For product packaging. 3. Design Key Points of the Design Product: The combination of shape and pattern. 4. Picture or Photo Best Showing the Design Key Points: Front View. 5. The Design Product is a flat product, and the left view, right view, top view, and bottom view are omitted.
Owner:SICHUAN NONGFUXIANG FOOD CO LTD

Fixing mold for bullfrog treatment

The utility model relates to a bullfrog processing fixing die which comprises a square frame, positioning notches allowing two forelimbs of a bullfrog to be embedded are formed in the two long side walls of a front end opening of the square frame, and front pressing plates sliding up and down are arranged at the positions, at the front end opening, of the two side walls of the square frame respectively. A rear pressing plate is arranged on a rear port of the square frame in a front-back sliding mode, a clamping hook structure capable of limiting backward movement of the rear pressing plate relative to the square frame is arranged on the rear pressing plate, the clamping hook structure is assembled on the rear pressing plate in a rotating mode, and a clamping hook is arranged at the free end of the clamping hook structure. When bullfrogs are placed in the fixing mold for bullfrog treatment, the front limbs of the bullfrogs are directly pinched by hands and pressed down into the square frame, then the front pressing plate slides upwards to limit the two front limbs of the bullfrogs so as to prevent the two front limbs of the bullfrogs from being separated from the positioning notches, and then the rear pressing plate moves forwards relative to the square frame and is buckled and positioned; the bullfrogs can be quickly pressed and fixed between the front pressing plate and the rear pressing plate, time and labor are saved, and operation is convenient.
Owner:HUBEI HUANHUAN FOOD TECHNOLOGY CO LTD

Bullfrog breeding circulating water device

The utility model discloses a bullfrog culture circulating water device which comprises a culture pond, one end of the culture pond is communicated with a circulating mechanism, the circulating mechanism comprises a circulating box communicated with the culture pond, the culture pond is communicated with the circulating box through a first connecting pipe, and the side, close to the culture pond, of the first connecting pipe is communicated with a filtering mechanism. The filtering mechanism comprises a filtering box communicated with the first connecting pipe, a filtering piece is arranged in the filtering box, a transition cavity, a neutralizing cavity and a storage cavity are sequentially communicated with the interior of the circulating box, the transition cavity is communicated with the first connecting pipe, a neutralizing mechanism is arranged at the top of the neutralizing cavity, and one end of a second connecting pipe is communicated with the interior of the storage cavity; and the other end of the second connecting pipe is communicated with the culture pond. According to the bullfrog breeding device, the repeated utilization rate of bullfrog breeding water is improved, and therefore the bullfrog breeding cost is reduced.
Owner:ZHANJIANG EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI +1

Bullfrog feed granulator with cleaning effect

The bullfrog feed granulator with the cleaning effect comprises a machine body, the machine body is provided with a stirring cavity and an extrusion cylinder, the stirring cavity is communicated with the extrusion cylinder, a valve is arranged at the communication position of the stirring cavity and the extrusion cylinder, a feeding hole is formed in the upper end of the stirring cavity, and an installation opening is formed in the left side of the machine body. A mounting hole is formed in the upper end of the left side of the stirring cavity and communicates with the mounting opening, a first spray head is arranged in the mounting hole and communicates with a water inlet pipe, the water inlet pipe communicates with a hose, and a second spray head is arranged at the upper end of the stirring cavity and communicates with the hose. The device is provided with the mounting opening, the mounting hole, the first spray head, the water inlet pipe, the hose and the second spray head, when equipment needs to be cleaned, water can be directly sprayed out of the first spray head and the second spray head, then a stirring rod stirs water in a stirring cavity to clean the stirring cavity, and a push plate moves in a reciprocating mode to scrape residual raw materials in an extrusion cylinder; and residual raw materials can be discharged from the discharge hole or the extrusion hole, so that the use is convenient.
Owner:GUANGDONG KELU FEED CO LTD

Solid-liquid separation treatment equipment for bullfrog breeding waste

The invention relates to the technical field of solid-liquid separation treatment, in particular to bullfrog breeding waste solid-liquid separation treatment equipment which is characterized in that a first rotating shaft is fixedly mounted at the output end of the bottom of a double-head motor, rotating stirring blades are fixedly mounted on the side wall of the first rotating shaft, a spiral body is fixedly mounted on the side wall of the first rotating shaft, and a filtering barrel is arranged on the side wall of the spiral body; a first connecting cylinder is fixedly mounted on the side wall of the filter barrel, and a second connecting cylinder is fixedly mounted in the separation box, so that the flow resistance of liquid in pores of the filter barrel and gaps of solid particles is reduced, and meanwhile, water is promoted to overcome surface tension and adhesive force and is more quickly separated from the interior of a solid, so that the solid-liquid separation efficiency is greatly improved; compared with a traditional solid-liquid separation method, more wastes can be treated in a shorter time, the water content of the separated solid is remarkably reduced, the liquid is clearer, the efficiency and the quality of subsequent treatment of the solid and the liquid respectively are greatly improved, and the difficulty and the cost of subsequent treatment are effectively reduced.
Owner:ZHANGZHOU HAIZHIWEI BIOTECHNOLOGY CO LTD +1

Antioxidant peptide from bullfrog mucus and application thereof

The invention discloses an antioxidant peptide derived from bullfrog mucus and application of the antioxidant peptide. The amino acid sequence of the antioxidant peptide is KFLNVVSGLLTSKYR, and the molecular weight of the antioxidant peptide is 1724 g / mol. Polypeptides are identified from bullfrog mucus through a high performance liquid chromatography technology for the first time, an unreported antioxidant peptide with antioxidant activity is screened out, the antioxidant peptide is synthesized through a solid phase method, and the antioxidant peptide derived from bullfrog mucus has the advantages of being safe, non-toxic and good in water solubility and stability and has good application prospects. After the vitamin B3 is combined with the vitamin B3, the oxidation resistance is remarkably improved, and the vitamin B3 can be widely applied to preparation of foods, cosmetics, medicines or health-care products and has important economic value and social significance.
Owner:GUANGZHOU TAIWEI FEED CO LTD

Industrial aquaculture management system for antibiotic-free bullfrogs

The invention discloses an industrial aquaculture management system for antibiotic-free bullfrogs, and belongs to the technical field of aquaculture. The system comprises circulating water antibiotic-free treatment, antibiotic-free seedling cultivation, antibiotic-free cultivation management and a novel drainage vertical pipe system. The circulating water non-antibiotic treatment adopts a closed-loop flow, and antibiotics and ammonia nitrogen are efficiently removed through the working procedures of sewage tank microfiltration, ozone oxidation disinfection, constructed wetland biological photodegradation, aeration tank ammonia nitrogen removal, clean water tank plant bacterium adding purification reflux and the like; a staged plant nutrition and probiotic feeding mode is adopted for antibiotic-free seedling cultivation; the antibiotic-free cultivation management realizes antibiotic-free cultivation through density control, water quality and water temperature regulation and control, scientific feeding and animal health care; the novel drainage vertical pipe adopts a circular hole and vertical seam structure to prevent blocking. According to the method, whole-course antibiotic-free breeding is achieved, the survival rate in the seedling stage reaches 80% or above, the adult frog breeding period is about 120 days, the product is free of antibiotic residues, the national environmental protection and food safety specifications are met, and an efficient solution is provided for factory-like antibiotic-free bullfrog breeding.
Owner:DINGHU DISTRICT WANHUA GREEN FROG BREEDING FARM (INDIVIDUAL IND & COMMERCIAL HOUSEHOLD)

Digital PCR kit for detecting red-eared slider and bullfrog and application thereof

The application provides a digital PCR kit for detecting highland invasive species red-eared slider and bullfrog, a set of red-eared slider specific primer probes, which are composed of a primer pair for a specific segment of a red-eared slider mitochondrial CYTB gene and a red-eared slider specific TaqMan probe matched therewith; a set of bullfrog specific primer probes, which are composed of a primer pair for a specific segment of a bullfrog mitochondrial 12S rRNA gene and a bullfrog specific TaqMan probe matched therewith, and a set of internal standard (IPC) primer pairs and internal standard TaqMan probes for monitoring the nucleic acid extraction and amplification reaction process monitoring. The application is provided with the primer pair for the specific segment of the red-eared slider mitochondrial CYTB gene and the red-eared slider specific TaqMan probe, the primer pair for the specific segment of the bullfrog mitochondrial 12S rRNA gene and the bullfrog specific TaqMan probe, and a common internal standard to construct a triple detection reaction system, thereby avoiding the quantitative uncertainty caused by the single species detection in the prior art which needs to be carried out in multiple reactions or relies on a standard curve for relative quantification.
Owner:西藏自治区生态环境监测中心 +2

Lunch box bottom box (bullfrog)

1. The name of this design product: lunch box bottom box (bullfrog). 2. Purpose of this design product: used as the base box of take-out lunch boxes. 3. The design key point of this appearance design product lies in the shape of the product. 4. The picture or photo that best illustrates the key points of the design: Design 1 Stereoscopic drawing 1. 5. Designate Design 1 as the base design.
Owner:WEIHAI JUNZIFENG PACKAGING TECH CO LTD

Outdoor ecological bullfrog culture pond

The utility model discloses an outdoor ecological bullfrog culture pond, which belongs to the technical field of aquaculture, aims to solve the problem of water leakage during use due to insufficient waterproof measures of the outdoor ecological bullfrog culture pond, and comprises a first barrel support and a second barrel support. The two ends of the first barrel support are movably connected with the two ends of the second barrel support through fixing mechanisms respectively, the first barrel support and the second barrel support which are connected form a pond body shell, and a waterproof mechanism is contained in the pond body shell to form a culture pond. The pond body shell and the waterproof mechanism are matched to form the culture pond, the waterproof mechanism can improve waterproof measures of the culture pond, the water leakage phenomenon is reduced in the using process, the applicability of the culture pond is improved, and the service life of the culture pond is prolonged.
Owner:TONGWEI AGRI DEV CO LTD

Premade fruit and vegetable sour soup braised crayfish, fruit and vegetable sauce and production method thereof

The invention belongs to the technical field of food processing, and particularly relates to pre-prepared fruit and vegetable sour soup braised crayfish, fruit and vegetable sauce and a production method of the pre-prepared fruit and vegetable sour soup braised crayfish. The preparation method comprises the following steps: mixing 15%-17% of rumex hanus, 20%-25% of passion fruits, 18%-20% of steamed pumpkins, 6%-7% of small lime, 15%-17% of carambola and 15%-17% of lemons according to the total weight of the fruit and vegetable sauce, crushing the mixture into paste, and frying the paste at 115-125 DEG C for 8-10 minutes; 0.5%-1% of table salt is added in the stir-frying process to form homogenized fruit and vegetable sauce with the pH value of 3.8-4.2, and then the fruit and vegetable sauce is used for producing the pre-prepared fruit and vegetable sour soup braised crayfish. According to the production method, local fresh fruits and vegetables in Hainan are adopted to prepare the instant sour fruit and vegetable sauce, so that the problems of fruit and vegetable skin shrinkage, water loss, core vitamin content attenuation and the like caused by transportation of fresh fruits and vegetables from other places are avoided; meanwhile, flavor upgrading and efficiency improving of the crayfish dish are achieved, and the method is particularly suitable for the scenes of chain catering and industrial production of the crayfish, the crabs, the shellfish, the fish, the bullfrog, the chicken and the beef braised with the sour soup and the oil by the prefabricated dishes.
Owner:HUBEI SHIYECAO AGRI TECH CO LTD

Feed additive for improving muscle quality of bullfrogs and application of feed additive

The invention relates to a feed additive for improving the muscle quality of bullfrogs and application of the feed additive. The additive is prepared from broad beans, radix scutellariae, rhizoma coptidis and radix et rhizoma rhei. According to the research, broad beans can remarkably improve the muscle quality of bullfrogs, meanwhile, radix scutellariae, rhizoma coptidis and radix et rhizoma rhei are added, so that potential negative effects of the broad beans on the bullfrogs can be remarkably improved, the digestion ability of the feed is improved, and the survival rate and the weight gain rate of the bullfrogs are kept. Therefore, the invention provides a special feed additive for directionally improving the meat quality and taste of bullfrogs, which can realize collaborative optimization of quality-growth-health, and has important application value for high-quality green breeding of bullfrogs.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY +2

Application of epicatechin in preparation of medicine for resisting frog iridovirus

The invention provides application of epicatechin in preparation of a medicine for resisting frog iridovirus. Related experiments prove that the epicatechin can efficiently prevent and control infection and transmission of the frog iridovirus, so that the harm of the frog iridovirus to healthy culture of frogs such as bullfrogs can be reduced to a great extent, and high-quality ecological culture and economic benefits of the frog culture industry are promoted.
Owner:GUANGXI XINONG INTELLIGENT AGRI TECH DEV CO LTD +2

Three-section type bullfrog cutting device

The utility model discloses a bullfrog three-section type cutting device, and particularly relates to the technical field of bullfrog cutting, which comprises a working plate, two supporting plates arranged at the top of the working plate, a connecting frame arranged at the tops of the two supporting plates, a cutting mechanism arranged at the bottom of the connecting frame, and a collecting mechanism arranged on one side of the working plate. Clamping mechanisms are arranged on the surfaces of the two supporting plates, each clamping mechanism comprises a sliding groove formed in the surface of the corresponding supporting plate, a first motor is arranged on one side of each supporting plate, a first bidirectional screw is arranged at the output end of each first motor, one end of each first bidirectional screw extends into the corresponding sliding groove, and two first sliding blocks are arranged on each first bidirectional screw; a first electric telescopic rod is arranged on one side of the first sliding block. The bullfrog cutting device can clamp and fix bullfrogs according to the shapes and the sizes of the bullfrogs, the bullfrogs are cut into three sections when the bullfrogs are cut, and the bullfrog cutting efficiency of the bullfrog cutting device is improved.
Owner:SHANTOU HENGXING AQUATIC PRODUCTS CO LTD

A bulking device for bullfrog feed production

The utility model discloses a kind of bulking devices for bullfrog feed production, comprising: box, its bottom is set as opening, both sides are fixedly connected with L-shaped support;Base is connected in the bottom of two L-shaped supports;Steam dispersion enters component, is communicated and fixed in the left side of box;Round pipe, is communicated and fixed in the top of box.The utility model is provided with a series of structures, can be pre-crushed to caking raw material when bullfrog feed raw material is added Handle, and by stirring cooperation dispersed feed high-temperature steam mode, ensure the uniform effect of contact between feed and steam, to ensure the drying and curing speed of feed, and it is convenient to directly overall remove large-area shielding to box bottom after curing, to facilitate drying and curing feed directly with large-area passage mode Anti-blocking fast smooth discharge work, avoid the phenomenon that existing small-bore discharge pipe is easy to block, feed is difficult to discharge and discharge efficiency is low, improve discharge efficiency and stability.
Owner:JIANGMEN YUEHAI FEED

An antioxidant peptide derived from bullfrog mucus and application thereof

The application discloses an antioxidant peptide derived from bullfrog mucus and application thereof, and the amino acid sequence of the antioxidant peptide is KFLNVVSGLLTSKYR, and the molecular weight is 1724 g / mol. The polypeptide is identified from the bullfrog mucus by high performance liquid chromatography for the first time, and an unreported antioxidant peptide with antioxidant activity is screened out. The antioxidant peptide derived from the bullfrog mucus is synthesized by a solid phase method, and has the characteristics of safety, non-toxicity, good water solubility and stability. After being combined with vitamin B3, the antioxidant property of the antioxidant peptide is significantly improved, and the antioxidant peptide can be widely applied to preparation of cosmetics, medicines or health products, and has important economic value and social significance.
Owner:GUANGZHOU TAIWEI FEED CO LTD

Composition for promoting healthy growth of bullfrog and preparation method thereof

The present invention relates to a composition for promoting the healthy growth of bullfrogs and a preparation method thereof, and belongs to the technical field of feed additives. The present invention creatively utilizes Aspergillus oryzae and neutral protease to carry out bacterial enzyme synergistic fermentation on soybean meal, thereby preparing fermented soybean meal, and then a certain mass ratio of dandelion extract, chuanxiong extract and Centella asiatica extract are used to form a composite Chinese herbal medicine, and a certain mass ratio of astaxanthin and β-1,3-d-glucan are used to form a composite regulator, so that when the composition obtained by mixing the three components with other components is added to feed and applied to the daily feeding of bullfrogs, on the one hand, the three components can exert a good synergistic effect, effectively reducing the illness of bullfrogs, and on the other hand, the composition also plays an excellent role in promoting the growth and development of bullfrogs, achieving healthy growth.
Owner:FOSHAN XINTUN BIOLOGICAL SCI & TECH

Antimicrobial peptides

PendingUS20250332215A1Compound screeningAntibacterial agentsAntimikrobielle peptideMycobacterium smegmatis
Antimicrobial peptides (AMPs), small compounds that often exhibit broad spectrum antimicrobial activity, are garnering interest as potential therapeutics against antibiotic-resistant bacterial pathogens. Development of new AMPs is arduous due to the practical limitations of classical protein-based discovery approaches. A high throughput bioinformatics approach is described which is able to confirm identification of known AMPs from the North American bullfrog (Rana (Lithobates) castebeiana) genome, and a bioinformatics approach is used to develop new AMPs, as described herein as SEQ ID NOs: 1-178. The described AMPs exhibit antimicrobial activity against Mycobacterium smegmatis via microtitre broth dilution assays, indicating broader efficacy.
Owner:UVIC INDUSTRY PARTNERSHIPS INC +1

Novel simplified method for extracting and preparing bullfrog skin collagen

The invention provides a novel method for efficiently extracting and preparing collagen by taking bullfrog skin as a raw material. The process comprises the following steps. The preparation method comprises the following steps: 1) removing impurities from bullfrog skin, cleaning, chopping and homogenizing, stirring and soaking the bullfrog skin with an alkaline solution to remove grease and non-collagen in the bullfrog skin, and washing the bullfrog skin with pure water until the bullfrog skin is neutral; the preparation method comprises 1) preparing collagen powder, 2) hydrolyzing with pepsin and an acetic acid solution, and filtering to obtain a collagen hydrolysate, 3) precipitating to remove macromolecules and non-collagen containing pigment in the collagen hydrolysate by an alkali precipitation method, and centrifuging or filtering to obtain a collagen extracting solution, and 4) concentrating the collagen extracting solution by a membrane, and freeze-drying to obtain the high-purity collagen powder.
Owner:ZHONGYANG BIOTECHNOLOGY (SHANGHAI) CO LTD

DNA molecular marker, primer and detection method for bullfrog sex identification

The invention discloses a DNA molecular marker, a primer and a detection method for bullfrog sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the nucleotide sequences of a primer pair for detecting the molecular marker are as shown in SEQ ID NO: 2 and SEQ ID NO: 3. The molecular marker provided by the invention meets the identification requirements of minimally invasive living bodies, is not influenced by the tissue specificity and development stage of bullfrogs, and is low in cost and simple to use; the molecular marker not only can quickly and efficiently identify the genetic sex of bullfrogs, but also can promote the establishment of bullfrog unisexual fry breeding technology, and has a wide application prospect.
Owner:JIMEI UNIV

Drying equipment for bullfrog breeding feed processing

The utility model discloses drying equipment for processing bullfrog breeding feed, which belongs to the technical field of drying equipment and comprises a shell, and a cylindrical drying cavity is formed in the shell; the overturning mechanism comprises a rotating pipe rotationally connected to the inner wall of the rear side of the cylindrical drying cavity, the front side of the rotating pipe is fixedly connected with a plurality of connecting rods, the rear side of each connecting rod is fixedly connected with an overturning plate, a motor is installed on the rear side of the shell, and the rotating pipe is fixedly connected with the rotating pipe. The rear end of the rotating pipe penetrates through the inner wall of the rear side of the cylindrical drying cavity and is in transmission connection with an output shaft of the motor through a transmission assembly. In order to solve the use problem, the equipment is provided with a stirring mechanism and a drying mechanism, a hot air blower is started to enable a plurality of air spraying holes to spray out hot air, feed drying can be efficiently achieved, a motor is matched to drive a plurality of stirring plates to rotate, overall stirring of the feed is achieved, the fluidity of the feed is improved, and the drying effect is further improved.
Owner:GUANGDONG HENGWANG FEED CO LTD

Multi-strain insect protein and microalgae compounded bullfrog feed and preparation method thereof

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.
Owner:YINHAO BIOTECHNOLOGY (XIAMEN) CO LTD

Feed conditioner for bullfrog feed processing

The utility model discloses a feed conditioner for bullfrog feed processing, which comprises a conditioner main body, a cleaning mechanism and a stirring mechanism are arranged in the conditioner main body, the stirring mechanism is positioned on the inner side of the cleaning mechanism, the interior of the conditioner main body is communicated with a steam inlet pipe, and the steam inlet pipe is communicated with a steam outlet pipe. The cleaning mechanism comprises a water tank welded to one side of the conditioner body, the top of the water tank is fixedly connected with a pump body, the top of the pump body is fixedly connected with a spiral hose, the top of the spiral hose is fixedly connected with a guide pipe, and the guide pipe extends into the conditioner body and penetrates through the scraper. According to the feed conditioner for bullfrog feed processing, through the arrangement of the cleaning mechanism, an operator can more conveniently clean the inner wall of the conditioner and the stirring mechanism after feed conditioning, the phenomenon that too much gelatinized feed is left on the inner wall of the conditioner and the stirring mechanism to form a caking phenomenon is avoided, and the operator can more conveniently maintain the conditioner.
Owner:GUANGDONG HENGYUAN FEED IND CO LTD