The invention discloses an anti-aging sauce and a preparation method thereof. The anti-aging sauce is characterized by being prepared from the following raw materials in parts by weight: 100-110 parts of fish, 12-13 parts of fish bones, 8-9 parts of fish roes, 25-27 parts of fish vesicles, 80-90 parts of loquat juice, 30-32 parts of carrots, 15-20 parts of pea seedlings, 4-5 parts of bullfrogs, 4-5 parts of porphyra, 20-23 parts of castanea mollissima, 7-8 parts of seeds of Capsellae bursa-pastoris, 2-3 parts of brown sugar, 2-3 parts of sweet osmanthus powder, 1-2 parts of astragalus membranaceus, 2-2.3 parts of longspur epimedium, 3-4 parts of lycium barbarum leaves, 1-2 parts of eucommia ulmoides, 2-2.5 parts of grifola frondosa, 1-2 parts of red rhodobryum, 10-11 parts of tea, 8-10 parts of soybean oil, 300-320 parts of sweet soybean paste and 8-10 parts of a nutrition health liquid. Complete nutrients of the fish are reserved; the fish vesicles are rich in collagen, and can be solidified after being decocted with water and then cooled; the anti-aging sauce is smooth and delicious, contains a plurality of Chinese herbal medicines, and is capable of resisting ageing after being frequently eaten; and in addition, tremella, puerarin and the like capable of promoting the production of body fluid to relieve thirst are added to the nutrient health liquid in the anti-ageing sauce, so that thirst caused by eating the sauce is avoided.