25 results about "White poplar" patented technology

Provided is a PagHB7a gene related to the salt tolerance and drought tolerance of poplar. The gene is used to create new germplasms of salt-tolerant and drought-tolerant 84K poplar (Populus alba×Populus glandulosa). Being a candidate gene related to the salt tolerance and drought resistance of poplar, the PagHB7a gene provides an important gene resource for breeding new varieties of salt-tolerant and drought-resistant poplar, and can be directly used for molecular-assisted breeding of stress-resistant poplar. In addition, a primer pair for amplifying the candidate gene and a transgenic modification method for poplar are provided, which can evaluate the stress resistance of poplar on the molecular level; on this basis, varieties with remarkable stress resistance can be accurately and efficiently screened, thus effectively shortening the breeding period of poplar.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PaVAMP711-a protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-a protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-a gene. Through a gene editing technology, the PaVAMP711-a gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

This invention relates to a gene, MaYUCCA2, that promotes adventitious root development and its applications, belonging to the field of plant growth and development genetic engineering. The nucleotide sequence of the MaYUCCA2 gene is shown in Sequence 3; its amino acid sequence is shown in Sequence 4. This invention, by transferring MaYUCCA2 into *Populus alba*, produces transgenic poplar trees named MaYUCCA2-OE. Compared with non-transgenic poplar trees (WT), the results show that MaYUCCA2-OE has a significantly higher endogenous auxin content, and during asexual propagation tissue culture, adventitious roots are more easily formed and their number is significantly increased. Furthermore, the callus tissue of MaYUCCA2-OE transgenic poplar is more significantly enlarged and deeper red in color compared to WT, and it is more likely to induce the production of adventitious buds.

This invention discloses PCR primers and identification methods for distinguishing the sex of poplar trees from five major groups and the sex genotype of the white poplar group. This invention is based on the sex-determining gene in poplar trees. FERR Genes and FERR-R Specific primers were designed for each gene, and a PCR primer set for detecting the sex of poplar trees from five different groups was finally obtained through screening. PCR detection results of poplar materials with known sex using this primer set were completely consistent with the actual poplar sex phenotype, demonstrating that the PCR primer set provided by this invention can accurately identify the sex of poplar trees from the five different groups at the molecular level, making poplar sex identification more universal. Furthermore, the specific amplification provided by this invention... FERR The primer pairs for the gene can accurately identify the sex chromosome composition of poplar species. This invention has significant application prospects in the identification of male and female sexes in the five major poplar species, molecular breeding of poplars, and the control of female tree fluff.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

1. Name of the designed product: Electric vehicle frame (white poplar - hard fork). 2. Use of the designed product: Frame for electric vehicle. 3. Design points of the designed product: In shape. 4. Picture or photo that best indicates the design points: Perspective view 1.

The invention discloses PCR (Polymerase Chain Reaction) primers and an identification method for identifying female and male genders of five-region-named poplar and gender genotypes of poplar poplar. Specific primers are respectively designed according to the sex determining genes FERR gene and FERR-R gene of the poplar, finally, the PCR primer group for detecting the sex of the five-grade poplar is obtained through screening, and the PCR detection result of the PCR primer group on the known sex poplar material is completely consistent with the actual sex phenotype of the poplar. The PCR primer group provided by the invention is proved to be capable of accurately identifying the male and female sex of the five grades of the poplar from the molecular level, so that the male and female sex identification of the poplar is more universal; in addition, the primer pair for specifically amplifying the FERR gene provided by the invention can be used for accurately identifying the sex chromosome composition of the poplar pie species. The method has an important application prospect in the aspects of sex identification of the five-part poplars, molecular breeding of the poplars, prevention and treatment of flying catkins of female plants and the like.

The present invention discloses the application and method of the PtobZIP29 gene in regulating the wood development of white poplar. By overexpressing the PtobZIP29 gene in white poplar, the division activity of the vascular cambium of the poplar is promoted, and the formation of secondary xylem is accelerated, thereby obtaining transgenic white poplar germplasm with increased wood biomass and improved wood fast-growing ability. This provides a method and germplasm basis for the breeding of fast-growing and superior forest varieties, is conducive to improving the wood yield and economic benefits of economic forests, and also provides materials for the construction of shelter forests and urban and rural greening.

1. Name of the designed product: Electric vehicle (white poplar - hard fork). 2. Use of the designed product: used for vehicles, for people to ride. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view 1.

The invention provides an ultra-dry preservation method for poplar seeds, and belongs to the technical field of local preservation of plants. The method comprises the following steps: mixing white poplar seeds and silica gel according to a mass ratio of 1: (5-15), treating and drying at low temperature for 5 hours to 3 days to obtain ultra-dry seeds, and carrying out vacuum sealing storage at room temperature or low temperature. According to the method disclosed by the invention, the germination rate of the stored white poplar seeds can be remarkably increased, the storage condition is simple, the germination rate can be further increased to 89% through rewetting treatment under normal-temperature storage, and natural storage can be realized on the premise of not needing other means, so that after the seeds are harvested, ultra-dry seeds are obtained by a user through silica gel treatment, and the germination rate of the white poplar seeds is increased. The high germination rate can still be obtained after long-term storage in the natural environment, and the method is economical and practical and has high application and popularization value.

The present invention discloses PCR-specific primers, a detection method, and a detection kit for detecting the genome of Populus alba. Based on the genomes of Populus alba, Xinjiang Populus, Populus davidianus, and Populus tremula, the present invention finds fragments with large differences in the genome through whole-genome sequence comparison analysis, and designs specific primers for each poplar accordingly, from which specific primers that can accurately distinguish the genome of Populus alba from the genomes of other poplars are screened. The present invention further provides a PCR detection method for quickly distinguishing Populus alba from other poplar species using the specific primers. The present invention can quickly distinguish Populus alba from other poplar species. The PCR detection method used is easy to operate, has a simple banding pattern, and can distinguish Populus alba-specific genomic fragments directly by amplifying fragments without sequencing. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The present invention discloses PCR primers, a detection method, and a PCR detection kit for detecting the genome of Xinjiang poplar. Based on the genomes of Populus alba, Xinjiang poplar, Populus rapa, and Populus tremula, the present invention finds fragments with large differences in the genome through whole genome sequence comparison analysis, and designs specific primers for each poplar accordingly, from which specific primers that accurately distinguish the Xinjiang poplar genome from the genomes of other poplars are screened. The present invention further provides a PCR detection method and a detection kit for quickly distinguishing Xinjiang poplar from other poplar species using the specific primers. The present invention can quickly distinguish Xinjiang poplar from other poplar species. The PCR detection method is easy to operate and has a simple banding pattern. It does not require sequencing to directly distinguish Xinjiang poplar-specific genomic fragments by amplifying fragments. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The invention belongs to the technical field of machine learning, and provides a poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation, and the main scheme is as follows: constructing a poplar stress-resistant phenotype prediction model based on machine learning, screening out poplar with stress-resistant phenotype based on the poplar stress-resistant phenotype prediction model, and storing the poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation. The tissue is used as a populus albus callus tissue material; the method comprises the following steps: determining pre-culture medium components of a populus albus callus material based on molecular dynamics simulation, and performing pre-culture treatment on the populus albus callus material by using a determined pre-culture medium; a cryoprotectant is screened based on molecular dynamics simulation, and the screened cryoprotectant is used for conducting freezing treatment on the populus albus callus material subjected to pre-culture treatment; and determining a thawing mode based on molecular dynamics simulation, and unfreezing and re-culturing the frozen populus albus callus material by using the determined thawing mode.

The present invention relates to the field of genetic engineering technology, and specifically to a PagMOM1 Genes and their applications in wood improvement. PagMOM1 The nucleotide sequence of the gene is shown in SEQ ID No. 1. Populus alba×Populus glandular A gene in Populus argentatus (84K) PagMOM1 , knocked out using CRISPR / Cas9 gene editing technology PagMOM1 Genes can promote the thickening of secondary cell walls, inhibit cell expansion, and change the characteristics of wood cells, thereby obtaining improved forest varieties with thickened secondary cell walls and smaller cells. mom1 mutant.

The invention relates to the technical field of tissue culture, in particular to a regeneration method of larix olgensis. According to the method, mature zygotic embryos of larix olgensis are subjected to callus induction, adventitious bud induction, elongation culture and transplanting. According to the method, the limitation that rooting and transplanting are difficult in the process of a larix olgensis tissue culture regeneration system is broken through, an efficient and rapid tissue culture regeneration system is achieved, the period is only about 6 months, a complete regenerated plant can be obtained, and callus induction, adventitious bud induction and elongation, the rooting rate and the transplanting rate are all improved. Technical support is provided for follow-up development of genetic improvement and industrial application of Larix olgensis trees, a feasible scheme reference is provided for cultivation of a precious new variety of Larix olgensis, and the method is also a breakthrough in the propagation technology level of Larix olgensis.

The invention relates to a populus tomentosa PtoYABBY2 gene and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the gene is as shown in Seq ID NO.1, and the gene encodes a protein of Seq ID NO.2. The gene can obviously advance the flowering time of plants by regulating and controlling key factors (such as up-regulation of AtFT and the like and inhibition of AtFLC) of a flowering pathway. Through overexpression of the PtoYABBY2 in arabidopsis thaliana, the flowering time can be advanced by about 4 days on average, and floral organs grow normally. The gene shows tissue specific expression (mature leaves > young leaves > stems / roots) and dynamic expression in a flower bud development stage in populus tomentosa. The invention provides a construction method of a cloning primer, a fluorescent quantitative primer and an overexpression vector. The invention also discloses a method for cultivating populus tomentosa varieties with different flowering time by using the gene. By applying the gene, the breeding cycle of populus tomentosa can be shortened, the problems of long juvenile phase and flying catkins pollution are solved, and a new tool is provided for forest molecular design breeding.

The invention discloses an ecological restoration management and control method and system for interplanting poplar and jerusalem artichoke in a windy and sandy area, and relates to the technical field of ecological engineering management and control, and the method comprises the steps: firstly collecting an environment parameter EP1 before planting, and calculating a wind erosion potential index W of each grid unit, so as to match differentiated planting planning parameters, and achieve the scientific layout according to local conditions; after planting, an environment parameter EP2 after planting is obtained, the daily compensation water amount ET is calculated, precise irrigation of the composite canopy is achieved, and the water resource utilization rate is increased. Meanwhile, a biological hazard index I is calculated, a patrol scheduling scheme APL is generated, and pest control is converted into active early warning from passive response. And finally, an irrigation scheduling scheme BPL is generated and sent to an automatic irrigation control terminal, a patrol scheduling scheme APL is sent to patrol personnel, key parameters are stored in a log data set HIS, a complete management and control process from data acquisition, scientific analysis to accurate execution is completed, and long-term health and stability of an ecological restoration project are ensured.

The present invention discloses PCR-specific primers and a PCR detection method for detecting the genome of Populus davidiana. The present invention is based on the genomes of Populus alba, Populus xinjiangensis, Populus siliquae and Populus davidiana, and through whole genome sequence comparison analysis, finds fragments with large differences in the genome, and accordingly designs specific primers for each poplar, from which specific primers that can accurately distinguish the genome of Populus davidiana from the genomes of other poplars are screened. The present invention further provides a PCR detection method for quickly distinguishing Populus davidiana from other Populus species using the specific primers. The method of the present invention can quickly distinguish Populus davidiana from other Populus species, and the PCR detection method is easy to operate, has a simple banding pattern, and can distinguish Populus davidiana-specific genomic fragments directly by amplifying fragments without sequencing. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a PaVAMP711-c protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-c protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-c gene. Through a gene editing technology, the PaVAMP711-c gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a PaVAMP711-b protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-b protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-b gene. Through a gene editing technology, the PaVAMP711-b gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.

The present invention discloses PCR specific primers and a PCR detection method for detecting the genome of Populus alba. The present invention is based on the genomes of Populus alba, Populus xinjiangensis, Populus sibiricus and Populus davidiana. Through whole genome sequence comparison analysis, fragments with large differences in the genome are discovered and specific primers for each poplar are designed accordingly. Finally, specific primers that can accurately distinguish the genome of Populus sibiricus from the genomes of other poplars are screened out. The present invention further provides a PCR detection method and a PCR detection kit for establishing a rapid distinction between Populus sibiricus and other Populus species using the specific primers. The present invention can quickly distinguish Populus sibiricus from other Populus species, is easy to operate, has a simple banding pattern, and can distinguish Populus sibiricus-specific genomic fragments directly by amplifying fragments without sequencing. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The invention discloses application of a PsAAAP21 gene in regulation and control of nitrogen utilization efficiency of poplar, and belongs to the technical field of molecular biology. The method comprises the following steps: constructing a plant inhibition expression vector containing a gene PsAAAP21, carrying out heterologous transformation on the plant inhibition expression vector to poplar 84K, screening to obtain a transgenic positive plant, and carrying out phenotypic analysis on the positive plant and a wild plant under different nitrogen treatment conditions to obtain a transgenic plant capable of efficiently utilizing nitrogen. The invention provides an important nitrogen efficient utilization gene resource possibly having universality, materials are provided for subsequent related research, and a foundation is laid for efficient utilization research of forest resources.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting taxol accumulation of taxus chinensis cells by overexpressing hydroxylase. The invention provides a method for promoting accumulation of paclitaxel in taxus chinensis cells, which comprises the following steps: constructing taxus chinensis cells overexpressing taxane-5 alpha-hydroxylase genes, performing tissue culture amplification, and extracting paclitaxel from the amplified taxus chinensis cells, wherein the taxane-5 alpha-hydroxylase from the overexpressed Popus alba has the optimal effect, and the yield of paclitaxel can be increased by 8.44%.