7 results about "White poplar" patented technology

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

This invention discloses PCR primers and identification methods for distinguishing the sex of poplar trees from five major groups and the sex genotype of the white poplar group. This invention is based on the sex-determining gene in poplar trees. FERR Genes and FERR-R Specific primers were designed for each gene, and a PCR primer set for detecting the sex of poplar trees from five different groups was finally obtained through screening. PCR detection results of poplar materials with known sex using this primer set were completely consistent with the actual poplar sex phenotype, demonstrating that the PCR primer set provided by this invention can accurately identify the sex of poplar trees from the five different groups at the molecular level, making poplar sex identification more universal. Furthermore, the specific amplification provided by this invention... FERR The primer pairs for the gene can accurately identify the sex chromosome composition of poplar species. This invention has significant application prospects in the identification of male and female sexes in the five major poplar species, molecular breeding of poplars, and the control of female tree fluff.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

The invention relates to the technical field of tissue culture, in particular to a regeneration method of larix olgensis. According to the method, mature zygotic embryos of larix olgensis are subjected to callus induction, adventitious bud induction, elongation culture and transplanting. According to the method, the limitation that rooting and transplanting are difficult in the process of a larix olgensis tissue culture regeneration system is broken through, an efficient and rapid tissue culture regeneration system is achieved, the period is only about 6 months, a complete regenerated plant can be obtained, and callus induction, adventitious bud induction and elongation, the rooting rate and the transplanting rate are all improved. Technical support is provided for follow-up development of genetic improvement and industrial application of Larix olgensis trees, a feasible scheme reference is provided for cultivation of a precious new variety of Larix olgensis, and the method is also a breakthrough in the propagation technology level of Larix olgensis.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.

The invention discloses application of a PsAAAP21 gene in regulation and control of nitrogen utilization efficiency of poplar, and belongs to the technical field of molecular biology. The method comprises the following steps: constructing a plant inhibition expression vector containing a gene PsAAAP21, carrying out heterologous transformation on the plant inhibition expression vector to poplar 84K, screening to obtain a transgenic positive plant, and carrying out phenotypic analysis on the positive plant and a wild plant under different nitrogen treatment conditions to obtain a transgenic plant capable of efficiently utilizing nitrogen. The invention provides an important nitrogen efficient utilization gene resource possibly having universality, materials are provided for subsequent related research, and a foundation is laid for efficient utilization research of forest resources.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting taxol accumulation of taxus chinensis cells by overexpressing hydroxylase. The invention provides a method for promoting accumulation of paclitaxel in taxus chinensis cells, which comprises the following steps: constructing taxus chinensis cells overexpressing taxane-5 alpha-hydroxylase genes, performing tissue culture amplification, and extracting paclitaxel from the amplified taxus chinensis cells, wherein the taxane-5 alpha-hydroxylase from the overexpressed Popus alba has the optimal effect, and the yield of paclitaxel can be increased by 8.44%.