52 results about "Poplar trees" patented technology

The invention provides application of a Pto-C3H35 gene haplotype in evaluation of poplar lignin content, and belongs to the technical field of molecular markers. According to the invention, the haplotype of the Pto-C3H35 gene is determined according to the nucleotides of SNP1, InDel1, InDel2, InDel3 and SNP2. The invention provides a primer group and a kit for determining the gene haplotype as well as a method for determining the gene haplotype, the lignin content of the poplar can be accurately judged by determining the genotype combination of the five sites, and superior plants with low lignin content are accurately and efficiently screened in the early growth stage of the poplar; the method is applied to new variety breeding of industrial poplar artificial forests, and effectively shortens the breeding period.

The invention relates to the technical field of biology, and particularly discloses application of a PagYUCCA6a gene in regulation and control of poplar wood yield. The PagYUCCA6a gene is cloned by taking a poplar variety 84K as a material and is constructed to an overexpression vector pMDC32, the gene is positioned behind a 35S promoter, and under the driving of the 35S promoter, the PagYUCCA6a can be overexpressed in a poplar body, so that the poplar wood yield is regulated and controlled, the PagYUCCA6a gene is a key gene for regulating and controlling the poplar wood yield, and the PagYUCCA6a gene can be used for regulating and controlling the poplar wood yield. The method has important application value in the field of forest high-yield gene engineering.

The present invention discloses a SNP molecular marker combination of poplar, a whole-genome liquid chip prepared therefrom, and applications thereof. The SNP molecular marker combination of poplar includes 60,944 SNP molecular markers, and the physical positions of the 60,944 SNP molecular markers are determined by sequence alignment based on the poplar reference genome GCA_015852605.2. The SNP molecular markers of poplar in the present invention can be used for specific identification of five major sections of poplar species. Using the SNP molecular markers of poplar to prepare a whole-genome liquid chip for poplar tree species can achieve efficient, accurate genotyping detection of low-cost poplar germplasm resource populations, and is specific to the sections of Populus, such as Leuce, Aigeiros, Tacamahaca, Leucoides, and Turanga, and has important application values in multiple fields of poplar biological breeding.

The invention discloses application of a PdePin1At protein and a coding gene thereof in regulation and control of a plant tree form. According to the invention, a transgenic poplar with reduced PdePin1At gene expression quantity is constructed by using a microRNA interference technology, and experiments prove that compared with a wild poplar, the leaf area, the leaf width, the leaf length, the branch included angle, the petiole length and the phyllotactic angle of the transgenic poplar are reduced, and the number of leaves / stem nodes is increased. The method is of great significance to molecular mechanism and theoretical research of regulating and controlling poplar tree forms, leaf shapes and quantity, branch angles and phyllotaxis, and is of great guiding significance to directional cultivation of trees with tree forms meeting actual production requirements. Under the condition that the plant height is not changed, the number of leaves of newborn organs can be increased, so that the number of photosynthetic organs is increased; and by changing the included angle of the branches, sunshine shielding can be reduced during close planting of the forest trees. Therefore, the method also has important guiding significance on agricultural production.

The invention discloses an application of PtoERD3 gene structure variation in evaluation of poplar lignin content, the structure variation is located in an upstream promoter region of a poplar PtoERD3 gene, is a 54bp chromosome structure variation SV fragment, and has a nucleotide sequence as shown in SEQ ID NO.1. The invention also discloses an application of the PtoERD3 gene structure variation in evaluation of poplar lignin content. By measuring the structural variation, the lignin content of the poplar can be accurately judged, superior plants with high-quality wood quality characters can be accurately and efficiently screened in the early growth stage of the poplar, the breeding period is effectively shortened, and a theoretical support is provided for molecular design breeding of the poplar wood quality.

The invention discloses a method for improving the character of a poplar, which is characterized in that a poplar GLR3.3b gene is knocked out through a CRISPR / Cas9 system to obtain a gene knockout poplar. Compared with a wild type poplar, the widths of a gelatinous layer, xylem in a stem, a cambium and a cortex are increased in a stem cell wall of the gene knockout poplar, and the cellulose content is remarkably improved, so that wood has higher strength and tensile strength, and the mechanical property of the poplar is improved.

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

This invention discloses PagUBC10a The application of genes in regulating the growth and development of poplar trees belongs to the field of agricultural bio-genetic engineering technology. PagUBC10a The application of genes in regulating the growth and development of poplar trees, the PagUBC10a The nucleotide sequence of the gene is shown in SEQ ID NO:1, and is utilized using the... PagUBC10a Genetically modified poplar trees are cultivated using the following methods: cloning poplar trees. PagUBC10a Genes; the aforementioned PagUBC10a The gene is linked to a vector to obtain an overexpression vector; the overexpression vector is transformed into Agrobacterium to obtain Agrobacterium bacterial suspension; the Agrobacterium bacterial suspension is used to inoculate poplar leaves to obtain... PagUBC10a Gene overexpression in poplar. This invention provides a new option for regulating the growth and development of poplar trees and offers a potential target for molecular breeding improvement of poplar traits.

The invention discloses application of a PagUBC10a gene in regulation and control of growth and development of poplars, belongs to the technical field of agricultural biological genetic engineering, and discloses application of the PagUBC10a gene in regulation and control of growth and development of poplars, the nucleotide sequence of the PagUBC10a gene is shown as SEQ ID NO: 1, transgenic poplars are cultivated by using the PagUBC10a gene, and a method comprises the following steps: cloning the PagUBC10a gene of the poplars; the PagUBC10a gene is connected to a vector, and an overexpression vector is obtained; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and impregnating poplar leaves with the agrobacterium liquid to obtain the PagUBC10a gene overexpressed poplar. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a potential target for molecular breeding improvement of the properties of the poplar.

This invention relates to a gene, MaYUCCA2, that promotes adventitious root development and its applications, belonging to the field of plant growth and development genetic engineering. The nucleotide sequence of the MaYUCCA2 gene is shown in Sequence 3; its amino acid sequence is shown in Sequence 4. This invention, by transferring MaYUCCA2 into *Populus alba*, produces transgenic poplar trees named MaYUCCA2-OE. Compared with non-transgenic poplar trees (WT), the results show that MaYUCCA2-OE has a significantly higher endogenous auxin content, and during asexual propagation tissue culture, adventitious roots are more easily formed and their number is significantly increased. Furthermore, the callus tissue of MaYUCCA2-OE transgenic poplar is more significantly enlarged and deeper red in color compared to WT, and it is more likely to induce the production of adventitious buds.

The invention discloses a visual detection system and detection method based on transgenic poplar. The visual detection system comprises an amplification primer, Cas12a protein and crRNA, a loop allosteric G-quadruplex, a suppressor and a scanning substrate. According to the method, an LAMP reaction is utilized to amplify a poplar transgenic fragment, CRISPR-Cas12a responds to an LAMP product and cuts a suppressor, a loop allosteric G-quadruplex can be combined with a heme post-catalysis substrate TBM to generate a yellow product in the absence of the suppressor, a scanner scans a chromogenic sample, RGB numerical values are collected, picture color information is analyzed, and the detection result is obtained. And the detection result can be stored in a high-throughput and digitalized manner. According to the detection method, complex instruments such as a thermal cycler are not needed, all related equipment can be carried about, a fixed power supply is not needed, and on-site and instant detection of the transgenic poplar in the field sampling process can be achieved.

The present invention discloses a method for knocking out genes in poplar trees. The method comprises using sterilized young leaves of poplar tissue culture seedlings as explants; infecting the explants with Agrobacterium, which is capable of expressing the Cas9 gene and a guide RNA for knocking out the poplar GLR2.8 gene via the CRISPR / Cas9 system; co-culturing the Agrobacterium-infected poplar explants; performing callus induction culture on the co-cultured poplar explants to obtain callus tissue; performing differentiation culture on the callus tissue to obtain a poplar plant with sprouts; and performing rooting culture on the sprouted poplar plant to obtain a gene-knocked-out poplar plant with sprouts and roots. The gene-knocked-out plant has improved mechanical properties. The present invention also discloses a method for preparing a composite material, comprising extracting wood fiber and bast fiber from the poplar plant obtained by the knockout method; and melt-blending the wood fiber and bast fiber with polylactic acid to obtain the composite material. The composite material has improved mechanical properties.

This invention discloses PCR primers and identification methods for distinguishing the sex of poplar trees from five major groups and the sex genotype of the white poplar group. This invention is based on the sex-determining gene in poplar trees. FERR Genes and FERR-R Specific primers were designed for each gene, and a PCR primer set for detecting the sex of poplar trees from five different groups was finally obtained through screening. PCR detection results of poplar materials with known sex using this primer set were completely consistent with the actual poplar sex phenotype, demonstrating that the PCR primer set provided by this invention can accurately identify the sex of poplar trees from the five different groups at the molecular level, making poplar sex identification more universal. Furthermore, the specific amplification provided by this invention... FERR The primer pairs for the gene can accurately identify the sex chromosome composition of poplar species. This invention has significant application prospects in the identification of male and female sexes in the five major poplar species, molecular breeding of poplars, and the control of female tree fluff.

The invention relates to a PagTTG1 gene for regulating and controlling pore opening and closing and drought resistance of poplar and application of the PagTTG1 gene, and relates to the technical field of stress-resistant biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagTTG1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagTTG1 gene is knocked out through CRISPR / Cas9, and compared with a wild plant, a TTG1 knockout plant has the advantages that the drought resistance is obviously enhanced, and the pore opening degree is obviously reduced; the WD family protein PagTTG1 gene is indicated to be a key regulatory factor for regulating and controlling pore opening and closing and drought resistance of the poplar. The invention provides a new choice for pore opening and closing and drought resistance regulation and control means, and has important application value in the field of forest stress resistance gene engineering.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

The present invention discloses a method for improving the traits of poplar trees. By using the CRISPR / Cas9 system, the GLR3.3a gene and the GLR3.3b gene of poplar trees are knocked out to obtain gene-edited poplar trees. Compared with the wild type, a gelatinous layer appears in the stem cell wall of the gene-edited poplar trees, the widths of the xylem, cambium and cortex in the stem increase, and the cellulose content is significantly increased, endowing the wood with higher strength and tensile strength and improving the mechanical properties of poplar trees.

This invention discloses PagBAG8 The application of genes in regulating the growth and development of poplar trees belongs to the field of forestry bio-genetic engineering technology. PagBAG8 The application of genes in regulating the growth and development of poplar trees, the PagBAG8 The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the gene utilizes the described... PagBAG8 The method for genetically breeding transgenic poplar trees includes the following steps: cloning poplar trees. PagBAG8 Genes; the aforementioned PagBAG8 Genes are linked to a vector to obtain an overexpression vector; the overexpression vector is transformed into Agrobacterium to obtain Agrobacterium culture; the Agrobacterium culture is then used to infect poplar leaves to obtain... PagBAG8 Gene overexpression in plants. This invention provides a new option for regulating poplar growth and development, and offers potential targets for molecular breeding improvement of poplar traits.

A device and method for rapid calculation of forest stock volume, belonging to the field of forest calculation technology, uses a drone as a platform and integrates a lidar module, an optical camera module, a positioning and navigation module, a data storage module, a data transmission module, and a ground control module to achieve rapid collection, storage, transmission, and preliminary processing of forest parameters. It employs a drone-borne lidar and optical camera working in tandem, using 10 poplar trees as a standard measurement sample to achieve rapid, accurate, and efficient calculation of poplar stock volume. This significantly reduces the labor costs and time required for forest stock volume calculation, enabling calculation of a single forest plot to be completed within 1-2 days with an error controlled within 5%. It is applicable to scenarios such as forestry resource inventory, forest tenure valuation, carbon sequestration, and forestry management, providing accurate and efficient data support for relevant departments.

This invention discloses a gene, PagSKP2a, related to poplar root growth and development. The coding region of this gene includes the sequence shown in SEQ ID NO.1. Overexpression of this gene significantly improves the rooting rate, taproot length, and lateral root number of poplar trees, which is of great significance for breeding poplar plants with easy rooting and well-developed root systems and for the innovation of poplar germplasm resources. This invention also discloses a breeding method for promoting poplar root growth and development and the application of the PagSKP2a gene in screening poplar varieties with easy rooting and well-developed root systems. It can be directly used for molecular-assisted breeding related to poplar root growth and development, effectively shortening the poplar breeding cycle.

The present invention provides a gene PtoCXE06 for poplar resistance to bark rot and its application, and relates to the field of molecular breeding technology. The present invention provides a gene PtoCXE06 for poplar resistance to bark rot, and by overexpressing the PtoCXE06 gene in poplar, the resistance of poplar to bark rot can be significantly improved. Experiments have confirmed that overexpressing the PtoCXE06 gene in white poplar can create a new poplar disease-resistant germplasm material with significantly enhanced resistance to bark rot, laying a foundation for the cultivation of new poplar varieties.

The invention discloses application of a PagWRKY40 gene in regulation and control of insect resistance of poplars, relates to the field of biotechnology and genetic engineering, and particularly relates to application of the PagWRKY40 gene or a protein coded by the PagWRKY40 gene in regulation and control of insect resistance of poplars, and a nucleotide sequence of the PagWRKY40 gene is shown as SEQ ID No.1. According to the invention, the PagWRKY40 gene of the 84K poplar is cloned, and an over-expression transgenic plant of the gene is obtained by utilizing an agrobacterium tumefaciens-mediated genetic transformation mode. Research results provide a basis for improvement of insect-resistant varieties of poplar trees and have important scientific significance for revealing excellent genes for forest tree genetic engineering breeding, and the 84K poplar PagWRKY40 gene has obvious insect-resistant ability and does not influence plant growth, so that the gene has very important application prospects in growth of transgenic plants, especially transgenic forest trees.

The present invention discloses a temperature control device that promotes the rooting of female flower branches of poplar trees while inhibiting flowering, including a heat preservation chamber, in which a constant temperature air conditioner is installed, and a sealing door is provided on the heat preservation chamber; the rooting part includes a water source tank, an outer shell is detachably connected to the water source tank, a first water pump is arranged in the water source tank, a net plate and a partition plate are fixedly connected in the outer shell, the upper part of the partition plate is the planting area, the lower part of the partition plate is the water storage chamber, a water volume adjusting component is arranged between the planting area and the water storage chamber, the first water pump is communicated with the water storage chamber, a water temperature adjusting component is arranged in the water storage chamber, an air temperature adjusting component is arranged outside the outer shell, the air temperature adjusting component is communicated with the planting area, a planting container mechanism is arranged on the net plate, and female flower branches of poplar trees are planted in the planting container mechanism. The present invention not only facilitates the control of the external environmental temperature of the female flower branches of poplar trees, but also facilitates the control of the temperature of the roots of the female flower branches of poplar trees, facilitates the rapid rooting of the female flower branches of poplar trees, and also avoids the disadvantages of hydroponics.

The invention discloses application of a poplar PdPXC1 gene in regulating poplar growth, xylem development and cell wall component synthesis. A nucleotide sequence of a coding region of the PdPXC1 gene is shown as SEQ ID NO: 1, and an amino acid sequence coded by the PdPXC1 gene is shown as SEQ ID NO: 3. A PdPXC1 over-expressed or gene-edited transgenic poplar plant can be obtained by constructing a recombinant expression vector containing the gene or a CRISPR / Cas9 gene editing vector and transforming the poplar. Compared with a wild type, the plant height, the ground diameter, the cambium cell number, the xylem width, the cellulose content, the lignin content and the fiber cell wall thickness of the overexpressed plant are all remarkably increased; and the gene editing plant shows an opposite phenotype. The invention provides an important gene target and a breeding material for directionally improving the growth speed of the poplar and the wood quality through a genetic engineering means, and has a wide application prospect in forestry production.

The invention discloses PCR (Polymerase Chain Reaction) primers and an identification method for identifying female and male genders of five-region-named poplar and gender genotypes of poplar poplar. Specific primers are respectively designed according to the sex determining genes FERR gene and FERR-R gene of the poplar, finally, the PCR primer group for detecting the sex of the five-grade poplar is obtained through screening, and the PCR detection result of the PCR primer group on the known sex poplar material is completely consistent with the actual sex phenotype of the poplar. The PCR primer group provided by the invention is proved to be capable of accurately identifying the male and female sex of the five grades of the poplar from the molecular level, so that the male and female sex identification of the poplar is more universal; in addition, the primer pair for specifically amplifying the FERR gene provided by the invention can be used for accurately identifying the sex chromosome composition of the poplar pie species. The method has an important application prospect in the aspects of sex identification of the five-part poplars, molecular breeding of the poplars, prevention and treatment of flying catkins of female plants and the like.

The invention discloses an H2A. Z1 protein and application of a coding gene of the H2A. Z1 protein in regulation and control of plant chromosome doubling. According to the invention, a model species poplar of a woody plant is taken as a research object, and a transgenic poplar with reduced H2A.Z1 gene expression quantity is constructed. Experiments prove that compared with wild poplar, the plant height and the stem node number of the transgenic poplar with reduced H2A.Z1 gene expression quantity are reduced, and the stem node length is increased. In addition, cells in stem and leaf organs of the transgenic poplar with reduced H2A.Z1 gene expression quantity become larger, cell chromosomes are doubled, and diploid is changed into tetraploid. The invention has important significance for researching polyploid breeding work of plants such as vegetables, important flowers, fruit trees and the like.

The invention discloses application of a PagSCL28a gene in regulation and control of poplar growth and development, belongs to the technical field of agricultural biological genetic engineering, and discloses application of the PagSCL28a gene in regulation and control of poplar growth and development, and the nucleotide sequence of the PagSCL28a gene is shown as SEQ ID NO: 1. The invention also discloses a method for cultivating a transgenic poplar by using the PagSCL28a gene. The method comprises the following steps: S1, cloning the poplar PagSCL28a gene; s2, the PagSCL28a gene is constructed into a plant expression vector, and a recombinant vector is obtained; s3, transforming the recombinant vector obtained in S2 into a poplar tissue culture seedling through an agrobacterium-mediated method, and screening to obtain a transgenic plant. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a key molecular target and a technical means for increasing the yield of poplar wood, optimizing the quality and improving the stress resistance.

The present invention discloses a method for improving the traits of poplar trees. The method is as follows: By means of genetic engineering, the MYB75 gene in poplar trees is highly expressed; improving the traits of poplar trees includes: increasing the stem diameter of poplar trees; promoting the growth of poplar tree leaves; promoting the growth of poplar tree roots; increasing the width of the xylem in the poplar tree stem; increasing the lengths of wood fibers and phloem fibers in the poplar tree stem. It lays a foundation for cultivating new materials of fast-growing, high-quality and high-yield poplar wood for plywood by molecular breeding to cultivate new varieties of high-quality and high-yield poplar wood for plywood.

The present invention discloses a method for improving the traits of poplar trees. The method is as follows: by means of genetic engineering, the PagKNAT5a gene in poplar trees is highly expressed; improving the traits of poplar trees includes: increasing the plant height of poplar trees; increasing the ground diameter of poplar tree plants; increasing the internode length of poplar trees; increasing the cell length of cortical cells in the xylem of poplar trees; increasing the fiber cell length of poplar tree plants; increasing the vessel length of poplar tree plants; increasing the width of the vascular xylem of poplar tree plants; increasing the cell wall thickness of xylem fiber cells in poplar tree plants; increasing the secondary cell wall thickness of the xylem of poplar tree plants; increasing the lignin content in poplar tree stem segments.

The present invention discloses a method for improving the traits of poplar trees. The method is as follows: by means of genetic engineering, the NAC73 gene in poplar trees is highly expressed; improving the traits of poplar trees includes: increasing the height of poplar trees; increasing the number of internodes of poplar trees; increasing the length of internodes of poplar trees; increasing the leaf area of poplar trees; increasing the width of the xylem of poplar trees; increasing the pore diameter of the xylem vessels of poplar trees. Cultivating fast-growing poplar trees lays a foundation for cultivating new varieties of fast-growing, high-quality and high-yield forest trees through molecular breeding.

This invention discloses PagSCL28a The application of genes in regulating the growth and development of poplar trees belongs to the field of agricultural bio-genetic engineering technology. PagSCL28a The application of genes in regulating the growth and development of poplar trees, the PagSCL28a The nucleotide sequence of the gene is shown in SEQ ID NO:1. And using the described... PagSCL28a The method for genetically breeding transgenic poplar trees includes the following steps: S1, cloning poplar trees PagSCL28a Gene; S2, will PagSCL28a Genes were constructed into plant expression vectors to obtain recombinant vectors; in step S3, the recombinant vectors obtained in step S2 were transformed into poplar tissue culture seedlings using Agrobacterium-mediated transformation, and transgenic plants were obtained through screening. This invention provides a new option for regulating the growth and development of poplar trees, and provides key molecular targets and technical means for improving poplar timber yield, optimizing quality, and enhancing stress resistance.