33 results about "Poplar trees" patented technology

The invention discloses application of a PdePin1At protein and a coding gene thereof in regulation and control of a plant tree form. According to the invention, a transgenic poplar with reduced PdePin1At gene expression quantity is constructed by using a microRNA interference technology, and experiments prove that compared with a wild poplar, the leaf area, the leaf width, the leaf length, the branch included angle, the petiole length and the phyllotactic angle of the transgenic poplar are reduced, and the number of leaves / stem nodes is increased. The method is of great significance to molecular mechanism and theoretical research of regulating and controlling poplar tree forms, leaf shapes and quantity, branch angles and phyllotaxis, and is of great guiding significance to directional cultivation of trees with tree forms meeting actual production requirements. Under the condition that the plant height is not changed, the number of leaves of newborn organs can be increased, so that the number of photosynthetic organs is increased; and by changing the included angle of the branches, sunshine shielding can be reduced during close planting of the forest trees. Therefore, the method also has important guiding significance on agricultural production.

The invention discloses an application of PtoERD3 gene structure variation in evaluation of poplar lignin content, the structure variation is located in an upstream promoter region of a poplar PtoERD3 gene, is a 54bp chromosome structure variation SV fragment, and has a nucleotide sequence as shown in SEQ ID NO.1. The invention also discloses an application of the PtoERD3 gene structure variation in evaluation of poplar lignin content. By measuring the structural variation, the lignin content of the poplar can be accurately judged, superior plants with high-quality wood quality characters can be accurately and efficiently screened in the early growth stage of the poplar, the breeding period is effectively shortened, and a theoretical support is provided for molecular design breeding of the poplar wood quality.

The invention discloses a method for improving the character of a poplar, which is characterized in that a poplar GLR3.3b gene is knocked out through a CRISPR / Cas9 system to obtain a gene knockout poplar. Compared with a wild type poplar, the widths of a gelatinous layer, xylem in a stem, a cambium and a cortex are increased in a stem cell wall of the gene knockout poplar, and the cellulose content is remarkably improved, so that wood has higher strength and tensile strength, and the mechanical property of the poplar is improved.

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

This invention discloses PagUBC10a The application of genes in regulating the growth and development of poplar trees belongs to the field of agricultural bio-genetic engineering technology. PagUBC10a The application of genes in regulating the growth and development of poplar trees, the PagUBC10a The nucleotide sequence of the gene is shown in SEQ ID NO:1, and is utilized using the... PagUBC10a Genetically modified poplar trees are cultivated using the following methods: cloning poplar trees. PagUBC10a Genes; the aforementioned PagUBC10a The gene is linked to a vector to obtain an overexpression vector; the overexpression vector is transformed into Agrobacterium to obtain Agrobacterium bacterial suspension; the Agrobacterium bacterial suspension is used to inoculate poplar leaves to obtain... PagUBC10a Gene overexpression in poplar. This invention provides a new option for regulating the growth and development of poplar trees and offers a potential target for molecular breeding improvement of poplar traits.

The invention discloses application of a PagUBC10a gene in regulation and control of growth and development of poplars, belongs to the technical field of agricultural biological genetic engineering, and discloses application of the PagUBC10a gene in regulation and control of growth and development of poplars, the nucleotide sequence of the PagUBC10a gene is shown as SEQ ID NO: 1, transgenic poplars are cultivated by using the PagUBC10a gene, and a method comprises the following steps: cloning the PagUBC10a gene of the poplars; the PagUBC10a gene is connected to a vector, and an overexpression vector is obtained; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and impregnating poplar leaves with the agrobacterium liquid to obtain the PagUBC10a gene overexpressed poplar. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a potential target for molecular breeding improvement of the properties of the poplar.

This invention relates to a gene, MaYUCCA2, that promotes adventitious root development and its applications, belonging to the field of plant growth and development genetic engineering. The nucleotide sequence of the MaYUCCA2 gene is shown in Sequence 3; its amino acid sequence is shown in Sequence 4. This invention, by transferring MaYUCCA2 into *Populus alba*, produces transgenic poplar trees named MaYUCCA2-OE. Compared with non-transgenic poplar trees (WT), the results show that MaYUCCA2-OE has a significantly higher endogenous auxin content, and during asexual propagation tissue culture, adventitious roots are more easily formed and their number is significantly increased. Furthermore, the callus tissue of MaYUCCA2-OE transgenic poplar is more significantly enlarged and deeper red in color compared to WT, and it is more likely to induce the production of adventitious buds.

The present invention discloses a method for knocking out genes in poplar trees. The method comprises using sterilized young leaves of poplar tissue culture seedlings as explants; infecting the explants with Agrobacterium, which is capable of expressing the Cas9 gene and a guide RNA for knocking out the poplar GLR2.8 gene via the CRISPR / Cas9 system; co-culturing the Agrobacterium-infected poplar explants; performing callus induction culture on the co-cultured poplar explants to obtain callus tissue; performing differentiation culture on the callus tissue to obtain a poplar plant with sprouts; and performing rooting culture on the sprouted poplar plant to obtain a gene-knocked-out poplar plant with sprouts and roots. The gene-knocked-out plant has improved mechanical properties. The present invention also discloses a method for preparing a composite material, comprising extracting wood fiber and bast fiber from the poplar plant obtained by the knockout method; and melt-blending the wood fiber and bast fiber with polylactic acid to obtain the composite material. The composite material has improved mechanical properties.

This invention discloses PCR primers and identification methods for distinguishing the sex of poplar trees from five major groups and the sex genotype of the white poplar group. This invention is based on the sex-determining gene in poplar trees. FERR Genes and FERR-R Specific primers were designed for each gene, and a PCR primer set for detecting the sex of poplar trees from five different groups was finally obtained through screening. PCR detection results of poplar materials with known sex using this primer set were completely consistent with the actual poplar sex phenotype, demonstrating that the PCR primer set provided by this invention can accurately identify the sex of poplar trees from the five different groups at the molecular level, making poplar sex identification more universal. Furthermore, the specific amplification provided by this invention... FERR The primer pairs for the gene can accurately identify the sex chromosome composition of poplar species. This invention has significant application prospects in the identification of male and female sexes in the five major poplar species, molecular breeding of poplars, and the control of female tree fluff.

The invention relates to a PagTTG1 gene for regulating and controlling pore opening and closing and drought resistance of poplar and application of the PagTTG1 gene, and relates to the technical field of stress-resistant biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagTTG1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagTTG1 gene is knocked out through CRISPR / Cas9, and compared with a wild plant, a TTG1 knockout plant has the advantages that the drought resistance is obviously enhanced, and the pore opening degree is obviously reduced; the WD family protein PagTTG1 gene is indicated to be a key regulatory factor for regulating and controlling pore opening and closing and drought resistance of the poplar. The invention provides a new choice for pore opening and closing and drought resistance regulation and control means, and has important application value in the field of forest stress resistance gene engineering.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

This invention discloses PagBAG8 The application of genes in regulating the growth and development of poplar trees belongs to the field of forestry bio-genetic engineering technology. PagBAG8 The application of genes in regulating the growth and development of poplar trees, the PagBAG8 The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the gene utilizes the described... PagBAG8 The method for genetically breeding transgenic poplar trees includes the following steps: cloning poplar trees. PagBAG8 Genes; the aforementioned PagBAG8 Genes are linked to a vector to obtain an overexpression vector; the overexpression vector is transformed into Agrobacterium to obtain Agrobacterium culture; the Agrobacterium culture is then used to infect poplar leaves to obtain... PagBAG8 Gene overexpression in plants. This invention provides a new option for regulating poplar growth and development, and offers potential targets for molecular breeding improvement of poplar traits.

A device and method for rapid calculation of forest stock volume, belonging to the field of forest calculation technology, uses a drone as a platform and integrates a lidar module, an optical camera module, a positioning and navigation module, a data storage module, a data transmission module, and a ground control module to achieve rapid collection, storage, transmission, and preliminary processing of forest parameters. It employs a drone-borne lidar and optical camera working in tandem, using 10 poplar trees as a standard measurement sample to achieve rapid, accurate, and efficient calculation of poplar stock volume. This significantly reduces the labor costs and time required for forest stock volume calculation, enabling calculation of a single forest plot to be completed within 1-2 days with an error controlled within 5%. It is applicable to scenarios such as forestry resource inventory, forest tenure valuation, carbon sequestration, and forestry management, providing accurate and efficient data support for relevant departments.

This invention discloses a gene, PagSKP2a, related to poplar root growth and development. The coding region of this gene includes the sequence shown in SEQ ID NO.1. Overexpression of this gene significantly improves the rooting rate, taproot length, and lateral root number of poplar trees, which is of great significance for breeding poplar plants with easy rooting and well-developed root systems and for the innovation of poplar germplasm resources. This invention also discloses a breeding method for promoting poplar root growth and development and the application of the PagSKP2a gene in screening poplar varieties with easy rooting and well-developed root systems. It can be directly used for molecular-assisted breeding related to poplar root growth and development, effectively shortening the poplar breeding cycle.

The invention discloses application of a PagWRKY40 gene in regulation and control of insect resistance of poplars, relates to the field of biotechnology and genetic engineering, and particularly relates to application of the PagWRKY40 gene or a protein coded by the PagWRKY40 gene in regulation and control of insect resistance of poplars, and a nucleotide sequence of the PagWRKY40 gene is shown as SEQ ID No.1. According to the invention, the PagWRKY40 gene of the 84K poplar is cloned, and an over-expression transgenic plant of the gene is obtained by utilizing an agrobacterium tumefaciens-mediated genetic transformation mode. Research results provide a basis for improvement of insect-resistant varieties of poplar trees and have important scientific significance for revealing excellent genes for forest tree genetic engineering breeding, and the 84K poplar PagWRKY40 gene has obvious insect-resistant ability and does not influence plant growth, so that the gene has very important application prospects in growth of transgenic plants, especially transgenic forest trees.

The present invention discloses a temperature control device that promotes the rooting of female flower branches of poplar trees while inhibiting flowering, including a heat preservation chamber, in which a constant temperature air conditioner is installed, and a sealing door is provided on the heat preservation chamber; the rooting part includes a water source tank, an outer shell is detachably connected to the water source tank, a first water pump is arranged in the water source tank, a net plate and a partition plate are fixedly connected in the outer shell, the upper part of the partition plate is the planting area, the lower part of the partition plate is the water storage chamber, a water volume adjusting component is arranged between the planting area and the water storage chamber, the first water pump is communicated with the water storage chamber, a water temperature adjusting component is arranged in the water storage chamber, an air temperature adjusting component is arranged outside the outer shell, the air temperature adjusting component is communicated with the planting area, a planting container mechanism is arranged on the net plate, and female flower branches of poplar trees are planted in the planting container mechanism. The present invention not only facilitates the control of the external environmental temperature of the female flower branches of poplar trees, but also facilitates the control of the temperature of the roots of the female flower branches of poplar trees, facilitates the rapid rooting of the female flower branches of poplar trees, and also avoids the disadvantages of hydroponics.

The invention discloses application of a poplar PdPXC1 gene in regulating poplar growth, xylem development and cell wall component synthesis. A nucleotide sequence of a coding region of the PdPXC1 gene is shown as SEQ ID NO: 1, and an amino acid sequence coded by the PdPXC1 gene is shown as SEQ ID NO: 3. A PdPXC1 over-expressed or gene-edited transgenic poplar plant can be obtained by constructing a recombinant expression vector containing the gene or a CRISPR / Cas9 gene editing vector and transforming the poplar. Compared with a wild type, the plant height, the ground diameter, the cambium cell number, the xylem width, the cellulose content, the lignin content and the fiber cell wall thickness of the overexpressed plant are all remarkably increased; and the gene editing plant shows an opposite phenotype. The invention provides an important gene target and a breeding material for directionally improving the growth speed of the poplar and the wood quality through a genetic engineering means, and has a wide application prospect in forestry production.

The invention discloses PCR (Polymerase Chain Reaction) primers and an identification method for identifying female and male genders of five-region-named poplar and gender genotypes of poplar poplar. Specific primers are respectively designed according to the sex determining genes FERR gene and FERR-R gene of the poplar, finally, the PCR primer group for detecting the sex of the five-grade poplar is obtained through screening, and the PCR detection result of the PCR primer group on the known sex poplar material is completely consistent with the actual sex phenotype of the poplar. The PCR primer group provided by the invention is proved to be capable of accurately identifying the male and female sex of the five grades of the poplar from the molecular level, so that the male and female sex identification of the poplar is more universal; in addition, the primer pair for specifically amplifying the FERR gene provided by the invention can be used for accurately identifying the sex chromosome composition of the poplar pie species. The method has an important application prospect in the aspects of sex identification of the five-part poplars, molecular breeding of the poplars, prevention and treatment of flying catkins of female plants and the like.

The invention discloses an H2A. Z1 protein and application of a coding gene of the H2A. Z1 protein in regulation and control of plant chromosome doubling. According to the invention, a model species poplar of a woody plant is taken as a research object, and a transgenic poplar with reduced H2A.Z1 gene expression quantity is constructed. Experiments prove that compared with wild poplar, the plant height and the stem node number of the transgenic poplar with reduced H2A.Z1 gene expression quantity are reduced, and the stem node length is increased. In addition, cells in stem and leaf organs of the transgenic poplar with reduced H2A.Z1 gene expression quantity become larger, cell chromosomes are doubled, and diploid is changed into tetraploid. The invention has important significance for researching polyploid breeding work of plants such as vegetables, important flowers, fruit trees and the like.

The invention discloses application of a PagSCL28a gene in regulation and control of poplar growth and development, belongs to the technical field of agricultural biological genetic engineering, and discloses application of the PagSCL28a gene in regulation and control of poplar growth and development, and the nucleotide sequence of the PagSCL28a gene is shown as SEQ ID NO: 1. The invention also discloses a method for cultivating a transgenic poplar by using the PagSCL28a gene. The method comprises the following steps: S1, cloning the poplar PagSCL28a gene; s2, the PagSCL28a gene is constructed into a plant expression vector, and a recombinant vector is obtained; s3, transforming the recombinant vector obtained in S2 into a poplar tissue culture seedling through an agrobacterium-mediated method, and screening to obtain a transgenic plant. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a key molecular target and a technical means for increasing the yield of poplar wood, optimizing the quality and improving the stress resistance.

This invention discloses PagSCL28a The application of genes in regulating the growth and development of poplar trees belongs to the field of agricultural bio-genetic engineering technology. PagSCL28a The application of genes in regulating the growth and development of poplar trees, the PagSCL28a The nucleotide sequence of the gene is shown in SEQ ID NO:1. And using the described... PagSCL28a The method for genetically breeding transgenic poplar trees includes the following steps: S1, cloning poplar trees PagSCL28a Gene; S2, will PagSCL28a Genes were constructed into plant expression vectors to obtain recombinant vectors; in step S3, the recombinant vectors obtained in step S2 were transformed into poplar tissue culture seedlings using Agrobacterium-mediated transformation, and transgenic plants were obtained through screening. This invention provides a new option for regulating the growth and development of poplar trees, and provides key molecular targets and technical means for improving poplar timber yield, optimizing quality, and enhancing stress resistance.

The device comprises a base plate, a fixing mechanism is arranged on the base plate, a first through hole is formed in the base plate, an outer cylinder is fixedly connected into the first through hole, an air inlet is formed in the outer cylinder, the air inlet is communicated with an air pump, a guide sleeve is fixedly connected into the outer cylinder, and the guide sleeve is fixedly connected with the outer cylinder. The bottom of the outer cylinder is fixedly connected with a rubber skirt; the bottom surface of the rubber skirt is fixedly connected with a plurality of sealing rings; the sampling assembly comprises an inner cylinder and a sampling cylinder, the inner cylinder is slidably connected into the guide sleeve, the sampling cylinder is slidably connected into the inner cylinder, a straight rod is arranged on the sampling cylinder, a bottom plate is fixedly connected to the inner cylinder, a power mechanism is arranged on the bottom plate, the power mechanism is in transmission connection with the straight rod, and a cutting mechanism is arranged on the sampling cylinder; a plurality of holding rods are fixedly connected to the bottom plate, and a connecting mechanism is arranged on the base plate. The rhizosphere soil collecting device can collect rhizosphere soil in water, can reduce the influence of water on a soil sample, and is more convenient to use.

Provided is PagAFP2a gene related to salt tolerance and drought tolerance of poplar trees. Under salt stress, PagAFP2a gene-knockout mutant poplar trees have a significantly enhanced antioxidant enzyme activity compared to that of WT (non-transgenic poplar trees), have significantly reduced reactive oxygen and malondialdehyde contents and relative conductivity, and have significantly enhanced resistance. However, the growth of the mutant poplar trees under normal culture conditions is not significantly different from that of WT. PagAFP2a gene-knockout mutants can significantly enhance salt tolerance and drought tolerance of poplar trees.

The invention discloses a stomata-specific promoter-driven gene expression vector and application thereof, and belongs to the technical field of plant genetic engineering. In order to improve the application value of ABA in forest tree breeding and improve the genetic improvement of poplar trees, the invention provides a stomatal specific promoter driver gene expression vector, and the expression vector comprises a stomatal specific promoter ProAtOST1 (SEQ ID NO.1) and a PagPYL4 gene (SEQ ID NO.2). According to the expression vector provided by the invention, the PagPYL4 gene can only respond to a drought signal in pore-related tissues, so that pore opening and closing are accurately regulated and controlled, and the water utilization efficiency is improved; the method has the advantages that adverse effects of constitutive overexpression on photosynthetic efficiency and biomass accumulation are avoided, synergistic regulation and control of drought resistance improvement, photosynthetic product accumulation and biomass increase are realized, and the method can be applied to plant genetic improvement, in particular to cultivation of drought-resistant poplar varieties.

Celluloses are taken from industrial trees by means of chemical pulp processing method which does not give damage to environment, and turned into fiber form, and fibers of industrial trees are turned into dental floss form. As said industrial tree; a kind of tree selected from beech tree, birch tree, poplar tree, elm and oak tree is used.

The invention discloses a gene for regulating a plant type and application thereof. The nucleotide sequence of a coding region of the PopLBO1 gene disclosed by the invention is as shown in SEQ ID NO: 1, and the amino acid sequence of the coded protein is as shown in SEQ ID NO: 2; the gene can negatively regulate and control the number of branches of the poplar and positively regulate and control the plant height and the first leaf node height of the poplar, and provides a key candidate gene and a theoretical basis for genetic improvement of the poplar plant type; the invention also discloses a method for regulating and controlling the poplar plant type and improving the poplar plant type by using the PopLBO1 gene, the poplar plant type can be subjected to directional genetic improvement by overexpressing or silently expressing the PopLBO1 gene in a poplar body, and excellent poplar germplasm adapting to different purposes can be quickly bred.

The utility model discloses a poplar seedling lateral bud rapid cleaning device which comprises two symmetrically-arranged arc-shaped sleeves, one ends of the arc-shaped sleeves are hinged, the hinged ends of the two arc-shaped sleeves are further connected with a long handle, cotton strips are arranged on the inner sides of the arc-shaped sleeves, cleaning inner sleeves are arranged on the inner sides of the cotton strips, and a plurality of liquid drainage holes are formed in the inner side surfaces of the cleaning inner sleeves. And a connecting spring embedded in the cotton sliver is also connected between the sleeve and the cleaning inner sleeve. Compared with the prior art, the poplar seedling lateral bud cleaning device has the advantages that when lateral buds are cleaned, the long handle is operated to enable the two arc-shaped sleeves to be arranged on a poplar trunk in a sleeved mode, and the poplar seedling lateral buds at different growth stages can be cleaned through the poplar seedling lateral bud cleaning device; meanwhile, when the lateral buds are cleaned, the cotton slivers absorb maleic hydrazide and daminozide agents, when the lateral buds are cleaned, the cotton slivers are extruded through the cleaning inner sleeve, the agents in the cotton slivers are discharged through the liquid discharging holes and smeared on the trunk of the poplar tree, the growth of the lateral buds is inhibited, and the working efficiency and effect are further improved.

This invention relates to a miRNA that regulates tree dwarfing and its application, belonging to the fields of plant genetic engineering and biotechnology; regulating tree dwarfing PagmiR396b The precursor sequence is shown in Sequence 1 of the sequence listing; it regulates tree dwarfing. PagmiR396b The mature sequence is shown in Sequence 2 of the sequence listing. This invention utilizes... PagmiR396b The precursor sequence was transferred into Silver Yang 84K and overexpressed. PagmiR396b Transgenic poplar trees with the precursor sequence showed a reduced plant height compared to non-transgenic poplar trees, indicating that... PagmiR396b The precursor sequence is key to regulating poplar dwarfing and has important application value in the field of forest tree dwarfing genetic engineering.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.

The application discloses a gene for regulating plant plant type and application thereof. The disclosed PopLBO1 gene has a coding region nucleotide sequence as shown in SEQ ID NO:1, and the amino acid sequence of the coded protein is as shown in SEQ ID NO:2. The gene can negatively regulate the branch number of a poplar tree, positively regulate the plant height and the first leaf node height of the poplar tree, and provides a key candidate gene and a theoretical basis for genetic improvement of the plant type of the poplar tree. The application further discloses a method for regulating the plant type of the poplar tree and improving the plant type of the poplar tree by using the PopLBO1 gene. By overexpressing or silencing expressing the PopLBO1 gene in the poplar tree, directional genetic improvement of the plant type of the poplar tree can be realized, and this is beneficial to fast breeding of excellent germplasm of the poplar tree suitable for different uses.