783 results about "Genotyping" patented technology

Provided are an apolipoprotein E detection reagent and the use thereof. The reagent comprises at least one of an ApoE2 protein detection reagent, an ApoE3 protein detection reagent and an ApoE4 protein detection reagent. One of a capture antibody and a detection antibody used for detecting ApoE2 protein or ApoE4 protein is a specific monoclonal antibody, and the capture antibody and the detection antibody used for detecting ApoE3 are both specific monoclonal antibodies. Genotyping of ApoE and quantitative detection of different genotypes of proteins are performed on the basis of an immunological detection method. The detection method has the advantages of being simple, easy to operate, short in time and cheap. A genotype detection result is highly consistent with a fluorescence PCR result. Genotyping of ApoE (6 types) can be performed, which is used to replace the nucleic acid detection and guide medication, and clarify the correlation of the ApoE4 genotype homozygosity / heterozygosity and the protein concentration of ApoE4 with AD.

The invention provides an SNP molecular marker combination for pop corn whole genome typing, a detection chip and application, and relates to the technical field of molecular breeding. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for genotyping of the whole genome of the pop corn, a genotyping object comprises 6419 SNP loci, the SNP markers uniformly cover the whole corn reference genome, and a high-quality molecular marker can be provided for map-based cloning of a pop corn specific agronomic trait control gene. Meanwhile, different types of corn can also be subjected to genotyping. Burst corn germplasm resources are narrower than other types of corn germplasm resources, and the chip can overcome the defect that an existing chip developed based on grain corn diversity is low in diversity in pop corn genotyping.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for constructing an oat DNA (Deoxyribose Nucleic Acid) fingerprint spectrum and application, the SNP molecular marker combination is developed and obtained based on an SLAFseq technology, and has the characteristics of uniformly covering an oat genome, being high in polymorphic information content and being suitable for typing. Compared with other traditional molecular marker technologies, the SNP marker provided by the invention has higher stability, is not easily influenced by experimental conditions and environmental factors, and can be widely applied to oat germplasm resource identification, genetic diversity analysis, DNA fingerprint construction and molecular marker-assisted breeding. The DNA fingerprint spectrum constructed on the basis of the SNP molecular marker can more comprehensively reflect genetic information of a biological individual or population, and is of great significance to research of genetic diversity, evolutionary relationship and the like of species. Therefore, the method can provide technical reference for popularization of oat varieties, property right confirmation and protection, purity identification of oat seed production and the like.

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

The invention relates to a lymphoma prognosis prediction method and device based on a PET-CT marker and a medium, and the method comprises the following steps: obtaining a PET / CT image of a patient, inputting the PET / CT image into a lymphoma prognosis prediction model based on image-clinical-molecular typing, and obtaining a prognosis risk category; the lymphoma prognosis prediction model based on image-clinical-molecular typing is constructed according to PET-CT markers, clinical indexes and genetic typing data, the PET-CT markers are obtained by calculating a segmentation result of a PET / CT image of a patient according to a bimodal lesion segmentation network, and prognosis significance verification is performed through a K-M survival curve; the bimodal lesion segmentation network comprises an encoder and a decoder, the encoder performs feature extraction through a plurality of convolutional layers, and a channel smoothing layer is introduced after the last convolutional layer to fuse the extracted features. Compared with the prior art, tumor information can be accurately captured from PET / CT images, and lymphoma prognosis prediction is carried out in combination with genomics and clinical medicine.

The invention discloses an SNP molecular marker related to the meat percentage character of procambarus clarkia and application of the SNP molecular marker, genetic typing is performed on the procambarus clarkia through whole genome association analysis, whole genome association analysis is performed on the meat percentage of the procambarus clarkia, and the SNP marker remarkably related to the meat percentage character is screened out and comprises one or more of SNP1-SNP10. By detecting the SNP marker, the procambarus clarkii with high growth speed and high meat percentage can be effectively predicted and bred, the breeding efficiency and accuracy of the procambarus clarkii are remarkably improved, a new variety of the procambarus clarkii with high yield and high quality is bred, and the SNP marker has a wide application prospect in the aspect of molecular breeding of the procambarus clarkii with the meat percentage character.

The invention discloses a tea tree liquid phase chip and application thereof, and relates to the technical field of molecular detection. The invention discloses a tea tree liquid phase chip and application thereof, according to site screening requirements and probe design principles, the tea tree liquid phase chip comprises 5781 SNP sites, and tea tree resource genetic typing can be realized based on a target interval genome sequence liquid phase capture accurate positioning sequencing typing technology. The tea tree liquid chip can realize low-cost genetic typing, is mainly specific to tea trees, can realize variety identification and genetic relationship analysis of the tea trees, scientifically guides hybridization improvement work of the tea trees and assists protection and development of germplasm resources of the tea trees, and has relatively high application values in multiple fields of tea tree breeding. The tea tree liquid phase chip can be used for tea tree genetic diversity evaluation, germplasm resource and genetic relationship identification, genetic map construction and gene localization, whole genome association analysis and tea tree molecular marker assisted breeding.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses a KASP molecular marker primer combination for detecting peanut late spot disease resistance and application of the KASP molecular marker primer combination, and belongs to the technical field of peanut molecular breeding. The molecular marker linked with the late spot resistance character can be used for detecting the genotype of peanuts so as to judge whether a material has late spot resistance or not, through high-throughput genotyping detection, manpower and material resources are greatly saved, the detection is not influenced by environmental factors, the result is accurate and reliable, and the molecular marker can be used for early screening of peanut late spot resistance varieties and can be applied to early screening of peanut late spot resistance varieties. The breeding period can be shortened and the breeding efficiency is improved.

The invention discloses a kit and a method for HLA cross reaction group and HPA genotyping. The system for HLA cross reaction group and HPA genotyping detection is obtained through respective combination design of two different primers on the basis of common specific SNP loci, and rapid, simple, low-cost and high-throughput HLA epitope and HPA genotyping detection can be realized, so that platelet infusion ineffectiveness and immune platelet infusion ineffectiveness are more effectively prevented.

The invention relates to an IGF2BP3 gene molecular marker primer related to pigeon weight traits and application of the IGF2BP3 gene molecular marker primer, and belongs to the technical field of biology. According to the invention, genome DNA of a to-be-detected pigeon is taken as a template, a specific primer is adopted to carry out PCR amplification on the genome DNA, then a PCR amplification product is subjected to Sanger sequencing and SNP molecular marker genotyping, and then pigeon body weight character selection is carried out. The weight of G / G genotype pigeons is higher than that of A / G and A / A genotype individuals, and the weight of A / G genotype pigeons is higher than that of A / A genotype individuals. By eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker is used as a genetic marker for breeding pigeons, and the pigeons with large weight and uniformity are bred; the method can efficiently and quickly identify the weight traits of the pigeons, improves the uniformity of the weight of pigeons, provides a scientific basis for the early breeding of the pigeons, and better serves the breeding of the pigeons.

The invention discloses a cucumber whole genome SNP (Single Nucleotide Polymorphism) site combination, a liquid chip and application of the cucumber whole genome SNP site combination. On the basis of whole genome re-sequencing data and GBS data of cucumbers, a group of SNP locus combinations and liquid phase chips which are uniformly distributed in the whole genome are developed, 17519 SNP loci are selected according to the whole genome re-sequencing data, 7340 SNP loci are selected according to the GBS data, and finally 24859 SNP loci are selected. 24859 SNP loci are uniformly distributed on the whole genome of the cucumber, so that an efficient whole genome genotyping tool is provided for genetic diversity analysis, variety identification, background screening, molecular marker-assisted breeding, gene positioning and whole genome association analysis of the cucumber, and the related research process of the cucumber is promoted. The method has an important application value.

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for genetic typing of micropterus salmoides and application of the SNP site combination. The SNP locus combination comprises 50000 SNP loci, and the positions and base information of the 50000 SNP loci on chromosomes are as shown in a table 3. By applying the micropterus salmoides whole genome liquid chip of the SNP site combination for genotyping of micropterus salmoides, provided by the invention, the genotype of a sample to be detected can be rapidly and accurately detected, and the average detection rate of the SNP sites and the average consistency rate of the genotype of the sample are as high as 99.95%; the micropterus salmoides whole genome liquid phase chip has the characteristics of high capture specificity, low cost, flexible sites and the like, is suitable for character breeding and improvement, gene mining, genetic relationship identification, genetic diversity analysis and the like of micropterus salmoides, and can effectively improve the breeding efficiency and accuracy of improved varieties of micropterus salmoides; and a new variety of largemouth micropterus salmoides which is more suitable for market requirements and is more competitive can be cultivated.

The invention discloses an application of a CYP3A4 * 1G gene in preparation of a product for evaluating the postoperative analgesic effect and dosage prediction of sufentanil compounded with butorenol. According to the invention, the gene polymorphism of CYP3A4 * 1G is found to be one of genetic factors causing sufentanil compounded butornori postoperative analgesic effect and dosage use difference individualized difference for the first time, and anesthetists can determine the dosage of analgesic drugs for postoperative patients according to genetic characteristic difference, so that the satisfaction degree of anesthesia and postoperative analgesia of the patients is improved; therefore, the CYP3A4 * 1G gene can be used as a marker for evaluating the postoperative analgesic effect and dosage use of sufentanil compounded with butoenorphan, and detection is carried out through related genes of postoperative analgesic medication of a patient and CYP3A4 * 1G genotyping, so that guidance can be provided for the postoperative analgesic medication dosage of the patient.

The invention discloses a specific molecular identity card for identifying a pier-head pig and application of the specific molecular identity card. The specific molecular identity card is a unique homozygous SNP (Single Nucleotide Polymorphism) site set of the pier-head pig. According to the method, the specific molecular marker of the pier-head pig is screened at the genome level through a molecular biological technology, and the symbolic genetic difference between the pier-head pig and other varieties can be revealed, so that the purpose of identifying the varieties is achieved. The method for obtaining the specific molecular marker of the pier head pig comprises the following steps: carrying out selective clearance analysis by calculating a genetic differentiation index (Fst) and a nucleotide polymorphism (pi) ratio, screening a selected region of the pier head pig, selecting a homozygous SNP site of the pier head pig in the region as candidate specific SNPs, carrying out genetic typing by virtue of a KASP typing technology, and screening out the specific molecular marker of the pier head pig. And counting the genotype frequency of each site in different varieties so as to screen out the specific molecular marker of the pier-head pig.

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention discloses an individual breast cancer risk detection method based on HRR pathway related genes, relates to the technical field of breast cancer risk detection, and aims to solve the problems of inaccurate breast cancer risk detection effect and unobvious subsequent treatment effect. According to the method, genetic typing and clinical data are combined to establish an adaptive model, relative risks are accurately calculated and graded, differential early warning and intervention schemes are formulated for low, medium and high risks, precision from risk detection to intervention is achieved, excessive medical treatment or insufficient intervention is avoided, prevention and control pertinence is improved, a dynamic tracking and optimization mechanism is established, and the risk detection accuracy is improved. Tracking frequency and indexes are set according to risk levels, intervention effects are evaluated regularly, schemes are adjusted, a'detection-intervention-tracking-optimization 'closed loop is formed, it is ensured that intervention measures continuously adapt to individual conditions, long-term risk management and control effectiveness is improved, and breast cancer prevention and control are promoted to be upgraded from static management to dynamic management.

The invention relates to an SNP molecular marker related to litchi fruit weight and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker related to the litchi fruit weight is based on a seedless litchi reference genome, the SNP molecular marker is located in a litchi SL05G0138630 gene coding region, and is located at the 27564818th basic group of a litchi chromosome 5, and the basic group is A or G. The application finds that a non-synonymous SNP site in a coding region of the litchi SL05G0138630 gene is closely associated with the litchi fruit weight phenotype, the variety with the weight of the litchi fruit can be efficiently identified by detecting the polymorphic site, the invention further discloses an identification method for the molecular level of the variety with the weight of the litchi fruit, and the molecular level of the variety with the weight of the litchi fruit can be identified by an SNP-based typing method. And cheap and accurate gene SNP typing can be achieved, so that the purpose of efficiently and accurately identifying the weight variety of the litchi fruit is achieved.

Disclosed are a genomic mating method for Huaxi cattle based on whole genome single nucleotide polymorphism (SNP) information and an application thereof. The method includes the following specific steps: step 1, extracting deoxyribonucleic acid (DNA) from to-be-hybridized Huaxi cattle individuals for genotyping; step 2, performing genotype data imputation to obtain high-density chip data; step 3, calculating an additive genetic relationship matrix, utilizing genomic best linear unbiased prediction (GBLUP) to obtain genomic estimated breeding values of five important economic traits of a to-be-hybridized Huaxi cattle population, and calculating a comprehensive selection index of the individuals; and step 4, using a genetic algorithm to construct a population optimal mating combination list. In the present invention, the breeding cost is greatly saved and an inbreeding level of offspring populations is reduced.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination, a molecular probe combination, a liquid phase chip and a kit for corn genotyping and application of the SNP molecular marker combination, the molecular probe combination, the liquid phase chip and the kit, and belongs to the technical field of gene chips. The SNP molecular marker combination and the liquid chip provided by the invention can be used for efficient and rapid genetic typing of corn germplasm resources, and are low in cost and suitable for corn biological breeding. The SNP marker provided by the invention uniformly covers the whole corn reference genome, provides a high-quality molecular marker for silage corn whole genome selective breeding, and can also be used for map-based cloning of important agronomic trait genes of silage corn.

The invention relates to the technical field of medical detection, in particular to a cervical cancer screening system based on risk HPV (human papillomavirus) expansion typing, and a risk assessment module provides solid technical support for individualized CIN2 + / 3 + risk assessment by using a Bayesian network and a Cox proportional risk model. By dynamically acquiring key data such as HPV types, cytological results, patient ages and infection duration, the Bayesian algorithm is beneficial to constructing individual specific risk models, and real-time risk division is carried out. The Cox regression model predicts accumulated risks in three years, dynamically adjusts a risk assessment threshold value and refreshes the weight of an input variable in real time in combination with a machine learning mechanism, and the composite model structure enables the risk assessment to be more flexible and ensures the scientificity of a management decision.

The invention provides a Beijing duck whole genome 60K SNP (Single Nucleotide Polymorphism) liquid phase chip, which comprises a reagent for detecting SNP sites, the SNP loci comprise highly selected SNP loci in identified Beijing ducks, Beijing duck production trait related genes obtained from transcriptome analysis and high-effect SNP loci in corresponding annotation files, and SNP loci which are mined according to existing GWAS research and are significantly related to production traits such as growth / body type of the Beijing ducks and potentially significantly related to the production traits such as growth / body type of the Beijing ducks. On the basis of large-scale meat duck groups in China, and on the basis of reasonably processed re-sequencing data and genetic typing, representative SNP and InDel loci are screened. The Beijing duck whole genome 60K SNP liquid phase chip can be used for Beijing duck genetic typing, auxiliary breeding, genetic relationship analysis, variety identification and the like.

The invention relates to the technical field of breeding methods, in particular to a precise breeding method based on single nucleotide polymorphism, which comprises the following steps: carrying out whole genome sequencing and screening SNP (Single Nucleotide Polymorphism) markers related to target traits; designing a genotype detection chip based on the SNP marker, and performing genotyping; constructing a genetic evaluation model and predicting a breeding value; designing a matching scheme based on the breeding value; performing performance verification on the offspring and continuously improving the breeding strategy. By integrating whole genome sequencing, transcriptome and metabolome data and combining bioinformatics and an artificial intelligence algorithm, efficient screening of functional SNPs related to complex characters is achieved, and the problems of multi-character collaborative improvement and insufficient environmental adaptability in a traditional method are effectively solved.

The invention relates to the technical field of biology, in particular to an SNP molecular marker for identifying upland cotton fiber quality, a KASP primer group and application of the SNP molecular marker and the KASP primer group. The invention provides five SNP (Single Nucleotide Polymorphism) molecular markers XW1-XW5 which are obviously related to fiber quality, and accurate and stable genotyping can be realized through verification. Wherein the fiber length can be remarkably increased by excellent allelotypes corresponding to the markers XW1, XW2, XW4 and XW5; the excellent allelotype corresponding to the marker XW3 can significantly improve the micronaire value, provides a reliable tool for upland cotton fiber quality identification and molecular breeding, and has important application value.

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention relates to the technical field of life science, in particular to a method for constructing an atrophic gastritis-based SPEM lesion model, which comprises the following steps: S1, constructing an Slc7a11 conditional knockout mouse strain: carrying out cage hybridization on an Slc7a11 flox / flox mouse and an ATP4b-Cre transgenic mouse, and knocking out a gastric wall cell specific Slc7a11 gene; s2, carrying out genotyping identification; the identification method based on the atrophic gastritis SPEM lesion model and the construction method based on the atrophic gastritis SPEM lesion model comprise the following steps: S1, pathological model verification: taking a mouse gastric mucosa tissue slice, quantitatively analyzing the proportion of an SPEM region by adopting an immunofluorescence double-standard technology, and confirming Slc7a11 knockout induced SPEM pathological characteristics in combination with the existence of SPEM in the gastric mucosa; and S2, long-term pathology evolution monitoring. Through gastric wall cell specific Slc7a11 gene knockout and multiple PCR identification technologies, the initial response SPEM state after gastric mucosa damage is successfully simulated, and the kit is used for researching atrophic gastritis pathological mechanisms and evaluating drug intervention.

A method for sequencing can be used to monitor a recipient of an allograft. A sample comprising nucleic acid molecules can be provided from the recipient of the allograft. A plurality of nucleic acid molecules can be isolated from the sample and amplified. The plurality of nucleic acid molecules can be subjected to one or more amplification reactions using a plurality of primers to generate a plurality of DNA molecules. The plurality of primers can target at least 100 independent polymorphisms. The DNA molecules, or derivatives thereof, can be sequenced. The method may not require genotyping of the recipient.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention provides a multi-task brain glioma automatic segmentation and IDH genetic typing method based on SAM. Cooperative optimization of segmentation and typing tasks is achieved. The method comprises the following steps: acquiring a brain glioma multi-mode MRI image data set; preprocessing the data set to obtain a preprocessed standardized data set, and dividing the standardized data set into a training set and a test set according to a preset proportion; establishing a multi-task brain glioma automatic segmentation and IDH genotyping model based on SAM, wherein the model comprises a four-branch image encoder, a feature fusion module, a prompt encoder, a mask decoder and an IDH classifier; inputting the training set into the model for training, setting a joint loss function, and optimizing parameters of the model through the joint loss function to obtain an optimized model; and inputting the test set into the optimized model for segmentation and IDH typing and prediction to obtain the tumor segmentation precision and IDH typing accuracy of the optimized model.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.