Detection of nucleic acid reactions on bead arrays

Abstract

The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

Description

[0001] This application is a continuing application of: U.S. Ser. No. 60 / 135,123, filed May 20, 1999; 60 / 160,917, filed Oct. 22, 1999; 60 / 135,051, filed May 20, 1999; 60 / 161,148, filed Oct. 22, 1999; Ser. No. 09 / 517,945, filed Mar. 3, 2000; 60 / 130,089, filed Apr. 20, 1999; 60 / 160,027, filed Oct. 22, 1999; Ser. No. 09 / 513,362, filed Feb. 25, 2000; 60 / 135,053, May 20, 1999; Ser. No. 09 / 425,633, filed Oct. 22, 1999; and Ser. No. 09 / 535,854, filed Mar. 27, 2000, all of which are expressly incorporated by reference.FIELD OF THE INVENTION [0002] The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic a...

AI Technical Summary

Benefits of technology

[0061] In an additional aspect of the invention, is a kit for the detection of a NASBA reaction wherein the first enzyme is a reverse transcriptase, and the kit comp

Problems solved by technology

Target amplification involves the amplification (i.e. replication) of the target sequence to be detected, resulting in a significant increase in the number of target molecules.

Specificity, in contrast, remains a problem in many currently available gene probe assays.

It may be possible under some circumstances to distinguish targets with perfect complementarity from targets with mismatches; this is generally very difficult using traditional technology such as filter hybridization, in situ hybridization etc., since small variations in the reaction conditions will alter the hybridization, although this may not be a problem if appropriate mismatch controls are provided.

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