Detection of nucleic acid reactions on bead arrays
Inactive Publication Date: 2006-12-07
ILLUMINA INC
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[0061] In an additional aspect of the invention, is a kit for the detection of a NASBA reaction wherein the first enzyme is a reverse transcriptase, and the kit comp
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Target amplification involves the amplification (i.e. replication) of the target sequence to be detected, resulting in a significant increase in the number of target molecules.
Specificity, in contrast, remains a problem in many currently available gene probe assays.
It may be possible under some circumstances to distinguish targets wit
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[0080] The present invention is directed to the detection and quantification of a variety of nucleic acid reactions, particularly using microsphere arrays. In particular, the invention relates to the detection of amplification, genotyping, and sequencing reactions. In addition, the invention can be utilized with adapter sequences to create universal arrays.
[0081] Accordingly, the present invention provides compositions and methods for detecting and / or quantifying the products of nucleic acid reactions, such as target nucleic acid sequences, in a sample. As will be appreciated by those in the art, the sample solution may comprise any number of things, including, but not limited to, bodily fluids (including, but not limited to, blood, urine, serum, lymph, saliva, anal and vaginal secretions, perspiration and semen, of virtually any organism, with mammalian samples being preferred and human samples being particularly preferred); environmental samples (including, but not limited to, ai...
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Abstract
The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.
Description
[0001] This application is a continuing application of: U.S. Ser. No. 60 / 135,123, filed May 20, 1999; 60 / 160,917, filed Oct. 22, 1999; 60 / 135,051, filed May 20, 1999; 60 / 161,148, filed Oct. 22, 1999; Ser. No. 09 / 517,945, filed Mar. 3, 2000; 60 / 130,089, filed Apr. 20, 1999; 60 / 160,027, filed Oct. 22, 1999; Ser. No. 09 / 513,362, filed Feb. 25, 2000; 60 / 135,053, May 20, 1999; Ser. No. 09 / 425,633, filed Oct. 22, 1999; and Ser. No. 09 / 535,854, filed Mar. 27, 2000, all of which are expressly incorporated by reference.FIELD OF THE INVENTION [0002] The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic a...
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