Rice whole genome SNP chip and application thereof

A whole genome and chip technology, applied in the field of rice whole genome SNP chip and preparation, rice SNP chip field, can solve the problems of limited use, high data analysis requirements, no chip, etc.
CN102747138AActive Publication Date: 2012-10-24先正达集团股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
先正达集团股份有限公司
Publication Date
2012-10-24

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Abstract

The present invention discloses a rice whole genome SNP chip and an application thereof. A method for preparing the chip comprises: (1) obtaining a first class of probes on a chip, wherein sequencing is performed to obtain a parental genome sequence, resequencing data of other rice varieties in a public database are combined, a Nipponbare genome is adopted as a reference sequence, a MAQ software is adopted to match and analyze all the sequencing data, and finally a SNP marker is screened; (2) obtaining a second class of probes on the chip, wherein a rice function gene is obtained from the public database, sequence difference reflecting gene function is searched, and a SNP / INDEL probe is designed according to the sequence difference; (3) adopting an infinium chip manufacturing technology to produce a SNP chip; and (4) testing accuracy and application efficiency of the chip. The chip of the present invention can be applicable for rice germplasm resource molecule marker fingerprint analysis, seed authenticity detection, filial generation genotyping, and other related researches.
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Description

technical field

[0001] The invention relates to the fields of molecular biology, functional genomics, bioinformatics and genome breeding, and more specifically relates to a rice whole-genome SNP chip and a preparation method thereof, and also relates to the application of the rice SNP chip. Background technique

[0002] Molecular markers refer to the technical method of using macromolecular compounds, proteins and DNA, to mark genetic variation by differences among biological individuals. Since the difference between individuals of protein is smaller than that of DNA, and it is difficult to detect, most molecular markers use genetic polymorphisms at the DNA level, also known as DNA molecular markers. Molecular marker technology mainly refers to molecular biology techniques for detecting DNA variation. Molecular marker technology is mainly used in the following four aspects in crop research: (1) construction of genetic linkage map and gene mapping; (2) germplasm resource res...

Claims

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