94 results about "Snp markers" patented technology

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention discloses a citrus hybrid offspring authenticity identification method based on whole genome SNP (Single Nucleotide Polymorphism) analysis. According to the invention, a genome typing technology in a whole genome range is adopted, and a set of discrimination system capable of accurately identifying real hybrid offspring is established through high-density SNP (Single Nucleotide Polymorphism) marker analysis and an IBD (Identity by means of an algorithm. The method breaks through the limitation of a traditional method on hybrid filial generation identification, and provides reliable technical support for breeding practice.

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a body height character. The SNP marker is located at the 136651710 bp position of the No.3 chromosome of sheep, and is Cgt; and T base mutation (rs403130818) is carried out. Experiments show that the SNP marker developed by the invention has significant influence on the sheep body height character (Plt; 0.05), and the allele T has more excellent body height character, so that the SNP molecular marker in the MCRS1 gene can be applied to molecular marker breeding of the sheep body height character. The molecular marker provided by the invention is not limited by the age, sex and the like of the sheep, can be used for breeding excellent body height sheep varieties, and even can be accurately screened when the sheep are newly born, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

This invention belongs to the field of molecular marker development for vegetable agronomic traits and marker-assisted breeding technology, specifically relating to a specific marker suitable for screening and breeding watermelon varieties, providing a high-throughput auxiliary technology for subsequent variety protection and improved seed breeding. This invention discloses 44 core SNP markers suitable for watermelon population structure analysis and variety identification; it also discloses KASP primers for amplifying the above SNP markers; and it further discloses the application of the above molecular markers in watermelon population structure analysis and variety identification.

The invention belongs to the technical field of genetic engineering and pulmonary interstitial disease medicine, and particularly relates to an SNP marker for auxiliary diagnosis of idiopathic pulmonary fibrosis and application of the SNP marker. The invention discloses and verifies the application value of a specific marker combination composed of 76 SNPs in auxiliary diagnosis of idiopathic pulmonary fibrosis for the first time. By detecting the SNP spectrum, an important molecular tool and data basis can be provided for early discovery of idiopathic pulmonary fibrosis, disease assessment and even subsequent drug target mining. The invention further provides a kit for detecting the 76 SNP markers, the kit has high sensitivity and specificity, and effective diagnosis of idiopathic pulmonary fibrosis can be achieved by detecting the 76 SNP markers.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

The invention discloses a corn genome breeding 45K chip and application thereof, the chip covers 44, 637 SNP loci and 27 InDel loci, and the SNP loci are uniformly distributed on 10 chromosomes and part of scaffold of a corn genome and have rich polymorphism. Compared with an existing corn chip, the SNP marker of the 45K chip is derived from re-sequencing results of 507 corn varieties, has better variety representativeness, and contains 925 key functional gene loci for close planting and high yield and 96 corn variety authenticity identification loci; the method can be more effectively used for functional gene identification, genetic diversity analysis, heterosis group division, whole genome selection analysis and variety authenticity identification.

The application discloses a SNP marker combination for broiler breast intramuscular fat content prediction and assisted breeding and application thereof, and belongs to the technical field of assisted breeding.The application aims to provide a method for broiler breast intramuscular fat content prediction and assisted breeding.The application provides 331 SNP markers related to broiler breast intramuscular fat content, which are used for identifying broiler breast intramuscular fat content.

The invention belongs to the technical field of aquatic genome selective breeding, and particularly relates to an edwardsiella tarda-resistant genome selective breeding liquid-phase chip for oplegnathus punctatus and application of the liquid-phase chip. According to the method, 500 core SNP loci highly associated with the Edwardsiella tarda resistance character are screened out from 2415773 high-quality SNPs by obtaining the whole genome SNP marker of the oplegnathus punctatus and combining the three methods of MME-rank, GWAS and PVE, and the low-density liquid phase chip prepared by taking the 500 core SNP loci as the core shows extremely high prediction accuracy in a reference group and an independent verification group and can be used for detecting the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character. The method is obviously superior to a whole genome set and a uniformly distributed marker set; family verification shows that the family GEBV calculated based on the chip is significantly related to the actual survival rate of the offspring. According to the method, high-precision disease resistance performance prediction is realized at extremely low cost, and a key technical tool and a solution are provided for rapid breeding of disease-resistant improved varieties of oplegnathus punctatus.

The application discloses a kind of silver carp variety specific SNP molecular marker combination, silver carp DNA fingerprint and application, the SNP molecular marker combination includes 367 SNP sites, the site information of the SNP molecular marker combination is as shown in table 1.The application takes silver carp germplasm resource as research object, obtains genotype by whole genome resequencing method, and then filters out 367 specific SNP markers capable of distinguishing different silver carp varieties, and the DNA fingerprint based on these markers can be used to distinguish different silver carp varieties.Compared with traditional methods, the fingerprint has the characteristics of high accuracy and strong reliability when used for silver carp variety identification.This technology not only provides strong support for the specific evaluation of silver carp varieties, the protection and management of breed rights, and the optimization and structure adjustment of silver carp breeding industry, but also shows good application prospects in the classification of silver carp germplasm resources, the confirmation of breed rights and the tracing of individual sources.

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

This invention belongs to the field of molecular biology detection, specifically relating to a rapid detection method for economic traits of Tibetan chickens. FBXO32 Gene SNP markers, detection methods, and applications. This invention is the first to discover: Tibetan chickens... FBXO32 The G / A mutation at position 8916 of the gene is closely related to the presence or absence of the Hpa II restriction site, and is also closely related to the main economic traits of Tibetan chickens. Based on this new discovery and the SNP site, this invention provides a method for rapidly detecting this SNP site and screening for economic traits in Tibetan chickens. The method is simple, convenient, fast, low-cost, and provides reliable results.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the body size and temperature adaptability of worker bees of apis cerana and application of the SNP marker. According to the invention, 25 key SNP sites and 10 associated genes for regulating and controlling the body type and temperature adaptation of the apis cerana are screened by a selection signal analysis method. Research finds that in the second intron region (6452066th site of the chromosome 7 of the bees) of the Foxo gene, the mutation type is T / C, and the mutation type is closely related to the body size and temperature adaptability of the Chinese bees. Through verification, in Hainan apis cerana and Aba apis cerana with extremely different body sizes and living environment temperatures, mutation position genotypes are different, TT is a large-body cold-resistant dominant genotype, and CC / TC is a small-body heat-resistant dominant genotype; a gene expression quantity detection result shows that the expression quantity of the Foxo gene is in negative correlation with a large body type and cold resistance and is in positive correlation with a small body type and heat resistance. The method can be used for genetic improvement of bee body shape characters and has breeding application value.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

The present application belongs to the technical field of plant molecular breeding, and particularly relates to a SNP marker combination for marker-assisted selection of apple fruit surface color degree, a probe set and application thereof. The SNP marker combination is composed of four SNP markers; the four SNP markers include SNP1 to SNP4; the nucleotide sequences of the SNP1 to the SNP4 are shown in SEQ ID NO. 1 to SEQ ID NO. 4 in turn. The present application provides a SNP marker combination for marker-assisted selection of apple fruit surface color degree, which can be applied to marker-assisted selection of apple fruit surface color degree traits at the seedling stage of apple hybrid offspring.

The invention discloses a method for auxiliary identification of Duroc pig growth traits based on a BTG1 region SNP marker, and belongs to the technical field of animal genetic breeding and reproduction. Comprising the following steps: step S1, acquiring a growth trait record of a Duroc pig to be detected, performing data preprocessing, and calculating genetic correlation and phenotypic correlation between heritability and growth traits based on a pedigree; step S2, performing genetic typing on the Duroc pig to be detected, and detecting genotypes of the Duroc pig in a BTG1 region and other key SNP sites; s3, carrying out auxiliary identification on the growth traits of the to-be-detected Duroc pigs on the basis of a pre-established relationship between genotypes and the growth traits; and S4, selecting a corresponding individual as a breeding object according to an auxiliary identification result. The method can be used for early screening and genetic improvement of growth traits such as different day-age weights and staged average daily gain of Duroc pigs, and is suitable for breeding pig breeding practice of large-scale pig farms, live pig breeding enterprises and scientific research institutions.

The application discloses a specific SNP marker for identifying a burley type in the genus Nicotiana and application thereof. A nucleotide sequence of a PCR amplification product of the specific SNP marker comprises a sequence as shown in SEQ ID No. 1. The specific SNP marker for identifying the burley type in the genus Nicotiana has the characteristics of stability, reliability, simplicity and low cost, and the molecular marker can effectively identify and detect whether the burley type variety exists in the genus Nicotiana.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker primer pair for distinguishing mandarin fish groups in Heilongjiang and Beijing water systems, a kit, application and a method. According to the invention, an SNP (Single Nucleotide Polymorphism) marker is found, the site is lgr4p.D874V and is located at the base 14537718 of a chromosome 1, and the base mutation type is T / A; different genotypes are shown in the two groups, and group verification shows that the site has remarkable group genetic differentiation characteristics and can be used as an effective molecular marker for subsequent group identification. On the basis, the invention also discloses a primer pair of the SNP marker, a kit containing the primer pair, and application of the SNP marker in molecular traceability, germplasm resource identification or population genetic characteristic analysis of a mandarin fish population. The invention further discloses a method for distinguishing siniperca chuatsi group sources in Heilongjiang and Beijing water systems and a molecular monitoring method for siniperca chuatsi local germplasm resources. The method can quickly and accurately identify the population source of the mandarin fish, judge the genetic background and guide the germplasm resource protection and improved variety breeding of the mandarin fish.

The invention discloses a method for identifying the purity of a cauliflower 'Youpon 65' hybrid as well as a molecular marker and application. According to the method, on the basis of double-parent re-sequencing data of the Youshon 65 variety, SNP loci with stable genetic difference between parents are obtained through screening, and two pairs of competitive allele specific PCR primers based on the KASP technology are developed respectively according to the SNP loci. By utilizing the two SNP markers and combining a fluorescence detection technology, the male parent, the female parent and the hybrid variety of the variety can be quickly and accurately distinguished in a single reaction. The method provided by the invention can be directly applied to purity identification and authenticity identification of the Youpon 65 commodity species, and provides a reliable technical basis for variety right protection and market supervision. Through early rapid identification of seed purity, production loss and market disputes caused by false hybrids can be effectively reduced, and identification efficiency and accuracy are significantly improved.

The present disclosure relates to SNP points and their combinations used in a diagnostic panel, to diagnose and genotype Brucella species in different samples by using genomic DNA of a single sample with SNP markers and their combinations to determine which or which of the bacterial species is infected with high accuracy and in a short time by performing multiplex reactions, to both reduce the labour force, keep the cost low when multiple samples are used, and prevent false or negative results due to the high sensitivity of the system used.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting the total flavor nucleotide content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify nine SNP markers associated with total flavor nucleotides. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing the total flavor nucleotide content, improving pork quality, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying a millettia specisoa champ germplasm resource, which comprises any one or a combination of any more of 384 SNP molecular markers, the physical positions of the 384 SNP molecular markers are determined by comparison based on a reference genome of millettia specisoa champ, the sites of the 384 SNP molecular markers are uniformly distributed on a millettia specisoa champ chromosome, and the number of the sites of the SNP molecular markers is MilSNP001-MilSNP384. The invention further discloses a specific probe corresponding to the SNP site, a detection method of the molecular marker, application of the molecular marker and a millettia speciosa champ breeding method. According to the 384 SNP markers provided by the invention, 1152 allelic variations are detected in 469 parts of germplasm resource materials in China, the average MAF value is 0.45, and the SNP markers have relatively high resolution on the germplasm resources of the beautiful millettia roots.

The application discloses a specific SNP marker for identifying sun-cured tobacco types in the genus Nicotiana and application thereof. A nucleotide sequence of a PCR amplification product of the specific SNP marker comprises a sequence as shown in SEQ ID No. 1. The specific SNP marker for identifying sun-cured tobacco types in the genus Nicotiana has the characteristics of stability, reliability, simplicity and low cost, and the molecular marker can effectively identify and detect whether there is a sun-cured tobacco type variety in the genus Nicotiana.

The invention belongs to the technical field of molecular biology, germplasm resource science and molecular breeding, and discloses an artemia 5K liquid phase chip based on a targeted capture sequence, a preparation method and application thereof, and the liquid phase chip comprises 4329 SNP sites. Compared with a previous fingerprint spectrum developed based on SNP molecular markers, the artemia liquid-phase chip disclosed by the invention contains more SNP markers, the identification accuracy is higher, the later detection cost is lower, the design is flexible, and interested marker sites can be added at any time in the later period.

The invention provides a construction method and application of a chrysanthemum aphid resistance prediction system based on whole genome selection. According to the method, SNP loci remarkably related to the aphid resistance of the chrysanthemum are identified through whole genome association analysis, the influence of different statistical models and SNP markers with different densities on the whole genome prediction precision is compared, the method for rapidly, efficiently and accurately predicting the aphid resistance of the chrysanthemum based on GWAS-assisted whole genome selection is established, and the prediction accuracy can reach 0.882. The whole genome selection system constructed based on the method can realize early selection of the aphid resistance of the chrysanthemum, accelerates the breeding process of the excellent new variety of aphid resistance of the chrysanthemum, saves the breeding cost, effectively overcomes the technical problems that the field aphid resistance identification work is tedious, long in period and easy to be influenced by environmental factors and human subjective factors and the like, and improves the identification efficiency of the aphid resistance of the chrysanthemum. The method has important theoretical and practical significance.

The invention discloses a shrimp individual inbreeding coefficient optimal evaluation parameter construction method based on a long homozygous fragment, and belongs to the technical field of molecular breeding of aquatic animals, and the method comprises the following steps: (1) carrying out SNP screening and typing on re-sequencing data of a shrimp sample by using a corresponding shrimp reference genome to obtain a corresponding number of whole genome SNP markers; (2) setting non-genome specific parameters and establishing a simulated sliding window database; (3) setting genome specific parameters; and (4) summarizing optimal evaluation parameters. The method does not depend on the quality of a reference genome, optimal parameters can still be found under the current fragmentation and low splicing quality conditions, ROH fragments are accurately detected, and the real inbreeding coefficient is accurately evaluated; meanwhile, the method can be popularized to other reference genomes with different splicing qualities, and corresponding optimal parameters are obtained.