Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs

A growth trait and marker technology, which is applied in the fields of animal molecular biotechnology and animal genetics and breeding, and can solve the problems of unidentified major genes and molecular markers.

Active Publication Date: 2014-07-02
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, so far, no major genes and molecular markers associated with growth traits have been identified in Chinese local pig breeds

Method used

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  • Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs
  • Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs
  • Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This example is to specifically explain the inventive process of obtaining the gene marker in the present invention.

[0020] Detection of 60K SNP genotype in the whole genome of pigs: A small piece of ear tissue sample was collected from each individual of the above two experimental groups, genomic DNA was extracted by standard phenol-chloroform method, and the DNA was dissolved in TE buffer. White Duroc × Erhualian pig F 2 The DNA of the resource population was tested for quality and concentration. The A260 / 280 ratio was 1.8-2.0, and the A260 / 230 ratio was 1.7-1.9. Qualified DNA samples were uniformly diluted to 50ng / μL -1 , using the Illumina Infinium SNP typing platform, order the Porcine SNP60DNA Analysis Kit chip, perform chip hybridization and result scanning according to the Illumina Infinium instructions and standard procedures, and read the genotype data through GenomeStudio software. The quality control of the obtained genotype data was carried out with PLI...

Embodiment 2

[0042] This example is the application of the SNP site g.34986810C>T obtained in Example 1 in breeding pigs. According to E Q Analysis of the distribution frequency of haplotypes in Chinese local pig breeds. For the core population of Chinese local pigs and the synthetic and matching lines of Chinese local pigs, the above-mentioned method of direct sequencing after PCR amplification was used to detect pig No. 7 The main mutation site g.34986810C>T genotype on the chromosome that affects the growth traits of pigs, select the favorable genotype TT to reserve seeds for individuals, and increase the live body length, live body height, carcass length, and Growth performance such as carcass weight, daily gain and head weight.

[0043] In this embodiment, the Erhualian group is used as experimental animals. Genomic DNA of 334 Erhualian pigs was directly sequenced after PCR amplification with specific primers. The genotype of g.34986810C>T main marker locus was determined according...

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Abstract

The invention provides a major SNP (single nucleotide polymorphism) marker influencing the growth traits of pigs. The SNP marker is located at a nucleotide sequence of an HMGA1 gene of a pig chromosome 7, a locus of the SNP marker is the nucleotide mutation of C857-G857 with an SEQ ID NO:1 sequence labeling position of 857, corresponding to a 34983991st nucleotide locus C> on chromosome 7 in a reference sequence in an international pig genome version 10.2, G mutation, or one of seven other loci completely linked with the locus. The invention also provides the application of the SNP marker in the genetic improvement of growth traits of breeding pigs, and provides the application of an SNP molecular marker with a linkage disequilibrium degree (r2) with the SNP marker of greater than 0.8 in the genetic improvement of growth traits of breeding pigs. The growth traits include one or more of the length and height of a living body of a pig, carcass length, carcass weight, daily gain and head weight. According to the invention, the breeding process of breeding pigs can be accelerated, the productivity of breeding pigs can be effectively improved, and remarkable economic benefits can be obtained.

Description

technical field [0001] The invention relates to the field of animal molecular biotechnology and animal genetics and breeding, and the main content is to identify major gene markers that can significantly increase the growth traits of pigs such as living body length, living body height, carcass length, carcass weight, daily gain, head weight and the like and the same Application in genetic improvement of breeding pig production performance. Background technique [0002] Growth traits are important indicators for evaluating the production performance of pigs, so they are important target traits in the genetic improvement research of breeding pigs. The traditional pig breeding method is evaluated by slaughtering the sibs or descendants of the target individual, which has high accuracy and remarkable effect, but has the shortcomings of high measurement cost, time-consuming and labor-intensive. Genetic improvement methods based on major gene molecular markers are economical, fas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 黄路生麻骏武任军丁能水周李生李琳
Owner JIANGXI AGRICULTURAL UNIVERSITY
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