6 results about "Gene probe" patented technology

The invention belongs to the technical field of tumor molecule detection, and particularly discloses a method and a kit for detecting HER2 gene mRNA (messenger Ribonucleic Acid) trace expression based on an RT-ddPCR (Reverse Transcription-Derived Polymerase Chain Reaction) technology. The kit contains a cross HER2 exon 19-20 / 20-21 specific primer probe, a reference gene specific primer probe and reverse transcriptase, and the ddPCR premix solution, the HER2 probe and the reference gene probe are respectively marked with different fluorophores. According to the detection method, after RNA extraction, reverse transcription and RT-ddPCR amplification of a detection sample are carried out, the HER2 / internal reference copy number ratio is calculated to realize typing, a breast cancer sample is completely negative when the Ratio is smaller than 1.0, is ultralow in expression when the Ratio is larger than or equal to 1.0 and smaller than 5.0, is low in expression when the Ratio is larger than or equal to 5.0 and smaller than 50.0, and is medium-high in expression when the Ratio is larger than or equal to 50.0. The detection sensitivity reaches 0.1 copy / microliter, absolute quantification can be achieved, adaptability to FFPE samples is high, breast cancer patients with ultralow expression of HER2 in IHC 0 can be accurately screened, a molecular basis is provided for accurate medication of ADC drugs such as Trastuzumab deruxtecan, and the kit can also be used for related detection of solid tumors such as gastric cancer and cervical cancer with HER2 gene amplification as a marker.

The invention relates to an NGS detection kit for gene variation related to targeted medication of gastric cancer and application of the NGS detection kit. The kit provided by the invention comprises a capture probe for detecting related genes and a corresponding reagent based on a next-generation sequencing technology, and variation conditions of six genes (BRAF, NTRK1, NTRK2, NTRK3, RET and ERBB2) of gastric cancer targeted medication related genes can be detected at one time by utilizing a hybrid capture method. The probe provided by the invention has the advantages of wide coverage and high sequencing depth, and can detect all variations in the capture area of the designed gene probe. According to the method, all variations of genes related to gastric cancer targeted medication can be reported, the specificity on gastric cancer patients is high, compared with multi-gene solid tumor large panel detection, resource waste caused by irrelevant gene detection is reduced, the detection cost is reduced, and the detection efficiency is improved.

The invention discloses a composition for detecting the methylation level of an EMBP1 gene and application of the composition. Comprising a detection gene primer pair and a detection gene probe, the detection gene primer pair has nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2 or nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4, and the detection gene probe has a nucleotide sequence as shown in SEQ ID NO: 11 or SEQ ID NO: 12. The composition is based on a qPCR method, can be used for detecting the methylation level of the EMBP1 gene, is high in detection sensitivity, good in specificity, good in sample applicability, suitable for non-invasive colorectal cancer screening, good in patient dependency and high in participation rate, and can provide an important basis for early diagnosis and treatment of colorectal cancer.

The embodiment of the invention provides a gene panel, a probe, a kit and a method for detecting bony mandibular prolapse, and belongs to the technical field of gene detection. The gene panel for detecting the bony mandibular prolapse comprises a plurality of target genes. According to the scheme, the reported bony mandibular preprocess related genes and the potential core pathogenic genes screened through the network propagation algorithm are integrated at the same time, a genetic detection panel which is wider in coverage and higher in relevance is constructed, and systematic capture and high-confidence identification of the bony mandibular preprocess pathogenic genes are achieved. The panel can detect functional mutation of multiple gene loci at a time, the problem that key genetic factors are omitted in traditional single-gene detection is solved, and the detection sensitivity and specificity are improved.

The invention provides a FISH probe combination and kit for detecting NECTIN4 gene amplification and a preparation method and application of the FISH probe combination and kit. The FISH probe combination comprises an NECTIN4 gene probe and a first chromosome internal reference probe; a preparation template of the NECTIN4 gene probe is composed of any one of the following BAC clone plasmids (1) to (3): (1) the BAC clone plasmids are RP11-157H6, CTD-3003O12 and RP11-4N6, and (2) the BAC clone plasmids are RP11-157H6, CTD-3003O12 and RP11-4N6; (2) cloning plasmids CTD-2299J15 and CTD-2502B20 by using a BAC (Bacterial Amplification Cellulose); and (3) cloning the plasmid CTD-2502B20 by using the BAC. The probe combination can detect the amplification condition of the NECTIN4 gene of a patient, provides data support for clinical tests and individualized treatment, has the characteristics of high sensitivity and good specificity, and can rapidly hybridize within 1 hour and stably interpret the signal intensity at high energy.