17 results about "Gene probe" patented technology

The invention provides a design method of a specific gene probe for detecting pathogenic microorganisms, and belongs to the technical field of microorganism detection. Comprising the following steps: acquiring reference genome data of target pathogenic bacteria, reference genome data of all species belonging to the same genus as the target pathogenic bacteria and genome data of all strains under the target pathogenic bacteria species; comparing the reference genome data of the target pathogenic bacteria with the reference genome data of all target pathogenic bacteria congeneric species by using MUMmer to obtain all fragments in the reference genome of the target pathogenic bacteria, wherein the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms, and the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms; recording starting base sites and ending base sites of all fragments; breaking a reference genome of the target pathogenic bacteria into a 50nt k-mer set, and filtering out non-specific fragments in the 50nt k-mer set to obtain an initial candidate probe set; filtering the initial candidate probe set to obtain a candidate probe set 3; and filtering the candidate probe set 3 to obtain a specific probe set. According to the design method, the specific probe is directly mined in the genomic data of the pathogenic microorganisms, large-scale probe design work can be competent, the designed probe has excellent resolution, the accuracy of pathogenic microorganism detection can be improved, and accurate detection of the pathogenic microorganisms is achieved.

The present invention relates to a respiratory pathogenic microorganism and drug resistance gene nucleic acid testing kit (gene chip method), comprising a kit body. The kit body comprises a PCR amplification reaction reagent, PCR amplification reaction consumables, gene chip hybridization reaction reagents, gene chip hybridization reaction consumables, and a specific gene probe. The gene chip hybridization reaction reagents include a hybridization solution and a 1000X cleaning solution; the gene chip hybridization reaction consumables include a gene chip and a coverslip; the PCR amplification reaction reagent is a PCR buffer solution; and the PCR amplification reaction consumables are PCR tubes. On the basis of gene chip technology in combination with novel multiplex PCR amplification technology, the present invention provides a novel respiratory pathogenic bacterium and drug resistance gene testing technology which has high testing throughput and sensitivity and good identification accuracy, is simple and easy to operate and low-cost, and is widely used as a conventional testing means; and testing results are used for guiding treatment plans and medications for patients suffering from respiratory diseases and even acute and severe patients.

The invention belongs to the field of digital PCR (polymerase chain reaction) detection of legionella pneumophila, and relates to a primer probe group, a kit and a detection method for detecting legionella pneumophila, primers comprise an upstream primer for detecting legionella pneumophila, a downstream primer for detecting legionella pneumophila, an internal control upstream primer and an internal control downstream primer; the probes comprise a legionella pneumophila detection probe and an internal control gene probe. According to the application, a sample with the concentration as low as 4000 copies / mL can be stably detected, the operation is simple, the detection efficiency is high, meanwhile, the legionella pneumophila is qualitative and quantitative, and secondly, the primer probe combination characteristic is high, and the detection sensitivity and the detection accuracy of the legionella pneumophila are high.

The invention belongs to the technical field of tumor molecule detection, and particularly discloses a method and a kit for detecting HER2 gene mRNA (messenger Ribonucleic Acid) trace expression based on an RT-ddPCR (Reverse Transcription-Derived Polymerase Chain Reaction) technology. The kit contains a cross HER2 exon 19-20 / 20-21 specific primer probe, a reference gene specific primer probe and reverse transcriptase, and the ddPCR premix solution, the HER2 probe and the reference gene probe are respectively marked with different fluorophores. According to the detection method, after RNA extraction, reverse transcription and RT-ddPCR amplification of a detection sample are carried out, the HER2 / internal reference copy number ratio is calculated to realize typing, a breast cancer sample is completely negative when the Ratio is smaller than 1.0, is ultralow in expression when the Ratio is larger than or equal to 1.0 and smaller than 5.0, is low in expression when the Ratio is larger than or equal to 5.0 and smaller than 50.0, and is medium-high in expression when the Ratio is larger than or equal to 50.0. The detection sensitivity reaches 0.1 copy / microliter, absolute quantification can be achieved, adaptability to FFPE samples is high, breast cancer patients with ultralow expression of HER2 in IHC 0 can be accurately screened, a molecular basis is provided for accurate medication of ADC drugs such as Trastuzumab deruxtecan, and the kit can also be used for related detection of solid tumors such as gastric cancer and cervical cancer with HER2 gene amplification as a marker.

The invention relates to an NGS detection kit for gene variation related to targeted medication of gastric cancer and application of the NGS detection kit. The kit provided by the invention comprises a capture probe for detecting related genes and a corresponding reagent based on a next-generation sequencing technology, and variation conditions of six genes (BRAF, NTRK1, NTRK2, NTRK3, RET and ERBB2) of gastric cancer targeted medication related genes can be detected at one time by utilizing a hybrid capture method. The probe provided by the invention has the advantages of wide coverage and high sequencing depth, and can detect all variations in the capture area of the designed gene probe. According to the method, all variations of genes related to gastric cancer targeted medication can be reported, the specificity on gastric cancer patients is high, compared with multi-gene solid tumor large panel detection, resource waste caused by irrelevant gene detection is reduced, the detection cost is reduced, and the detection efficiency is improved.

The invention belongs to the field of molecular diagnostic biology, and relates to a primer probe combination, a kit and a detection method for quantitatively detecting group B streptococcus nucleic acid, and the primer probe combination comprises an upstream primer for detecting group B streptococcus and has a nucleotide sequence as shown in SEQ ID NO: 1; the downstream primer for detecting the group B streptococcus has a nucleotide sequence as shown in SEQ ID NO: 2; the group B streptococcus detection probe has a nucleotide sequence as shown in SEQ ID NO: 3; the internal control gene upstream primer has a nucleotide sequence as shown in SEQ ID NO: 4; the internal control gene downstream primer has a nucleotide sequence as shown in SEQ ID NO: 5; and the internal control gene probe has a nucleotide sequence as shown in SEQ ID NO: 6. The method is simple to operate and high in detection efficiency, and can be used for qualitative and quantitative detection of group B streptococcus at the same time, and the primer probe combination is high in characteristic and high in detection sensitivity and detection accuracy of group B streptococcus.

The invention discloses an unequal-depth HRD and HRR gene hybridization capture probe composition as well as a preparation method and application thereof, and belongs to the technical field of molecular biology. The preparation method of the unequal-depth HRD and HRR gene hybridization capture probe composition comprises the following steps: mixing a streptavidin solution with a targeted HRD region probe group, and incubating to obtain a mixed solution; and mixing the mixed solution with a target HRR gene probe group to obtain the unequal-depth HRD and HRR gene hybridization capture probe composition, the ratio of each probe to the streptavidin in the targeted HRD region probe group is 1.6 fmol: (0.1-1) [mu] g. According to the invention, streptavidin is utilized to selectively close part of probes in the target HRD region probe group, and then the probe group is mixed with the target HRR gene probe group, so that the purpose of unequal-depth sequencing of the two regions is achieved without physical separation, and the cost is reduced by 50% compared with that of branched-tube hybridization capture.

The invention provides a detection device for plant pathogenic microorganisms, and belongs to the technical field of microorganism detection, the detection device contains a specific gene probe set of the plant pathogenic microorganisms, and the plant pathogenic microorganisms comprise at least one of Alternania alterata, Botrytis cinerea, Fusarium avenaceum, Fusarium bothii, Fusarium fujikuroi, Fusarium trichotricinctum, Fusarium trichotricinctum, Sclerotinia sclerotiorum and Verticilliumdahliae. The device comprises a specific gene probe set of plant pathogenic microorganisms, has excellent resolution, can improve the accuracy of pathogenic microorganism detection, and realizes accurate detection of soil-borne pathogenic microorganisms.

The invention belongs to the field of biological detection, and relates to a primer probe combination, a kit and a detection method for quantitatively detecting haemophilus influenzae, primers comprise an upstream primer for detecting haemophilus influenzae, a downstream primer for detecting haemophilus influenzae, an internal control upstream primer and an internal control downstream primer, the probe comprises a haemophilus influenzae detection probe and an internal control gene probe; the nucleotide sequence of the upstream primer for detecting haemophilus influenzae is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer for detecting haemophilus influenzae is as shown in SEQ ID NO: 2; the nucleotide sequence of the internal control upstream primer is as shown in SEQ ID NO: 3, and the nucleotide sequence of the internal control downstream primer is as shown in SEQ ID NO: 4; the nucleotide sequence of the haemophilus influenzae detection probe is as shown in SEQ ID NO: 5, and the nucleotide sequence of the internal control gene probe is as shown in SEQ ID NO: 6. According to the application, the qualitative and quantitative detection of haemophilus influenzae can be realized at the same time, and the sensitivity and accuracy of haemophilus influenzae detection are improved.

The invention discloses a composition for detecting the methylation level of an EMBP1 gene and application of the composition. Comprising a detection gene primer pair and a detection gene probe, the detection gene primer pair has nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2 or nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4, and the detection gene probe has a nucleotide sequence as shown in SEQ ID NO: 11 or SEQ ID NO: 12. The composition is based on a qPCR method, can be used for detecting the methylation level of the EMBP1 gene, is high in detection sensitivity, good in specificity, good in sample applicability, suitable for non-invasive colorectal cancer screening, good in patient dependency and high in participation rate, and can provide an important basis for early diagnosis and treatment of colorectal cancer.

The invention belongs to the field of molecular diagnostic biology, and relates to a primer, a probe, a kit and a detection method for quantitatively detecting streptococcus pneumoniae, the primer comprises an upstream primer for detecting streptococcus pneumoniae, a downstream primer for detecting streptococcus pneumoniae, an internal control gene upstream primer and an internal control gene downstream primer, the probe comprises a streptococcus pneumoniae detection probe and an internal control gene probe; the nucleotide sequence of the upstream primer for detecting the streptococcus pneumoniae is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer for detecting the streptococcus pneumoniae is as shown in SEQ ID NO: 2; the nucleotide sequence of the upstream primer of the internal control gene is as shown in SEQ ID NO: 3, and the nucleotide sequence of the downstream primer of the internal control gene is as shown in SEQ ID NO: 4; the nucleotide sequence of the streptococcus pneumoniae detection probe is as shown in SEQ ID NO: 5, and the nucleotide sequence of the internal control gene probe is as shown in SEQ ID NO: 6. According to the application, the sensitivity and accuracy of streptococcus pneumoniae detection can be improved; operation is simple and detection efficiency is high.

The invention belongs to the field of medical detection, and relates to a primer probe combination, a kit and a detection method for quantitatively detecting pseudomonas aeruginosa, primers comprise an upstream primer for detecting the pseudomonas aeruginosa, a downstream primer for detecting the pseudomonas aeruginosa, an internal control gene upstream primer and an internal control gene downstream primer, the probes comprise a pseudomonas aeruginosa detection probe and an internal control gene probe; the nucleotide sequence of the upstream primer for detecting the pseudomonas aeruginosa is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer for detecting the pseudomonas aeruginosa is as shown in SEQ ID NO: 2; the nucleotide sequence of the upstream primer of the internal control gene is as shown in SEQ ID NO: 3, and the nucleotide sequence of the downstream primer of the internal control gene is as shown in SEQ ID NO: 4; the nucleotide sequence of the pseudomonas aeruginosa detection probe is as shown in SEQ ID NO: 5, and the nucleotide sequence of the internal control gene probe is as shown in SEQ ID NO: 6. The method is simple to operate and high in detection efficiency, and the detection sensitivity and the detection accuracy of the pseudomonas aeruginosa are improved.

The invention discloses application of OPTN in treatment of NPM1 mutant leukemia, and relates to the technical field of biological medicines. Comprising application of OPTN as a biomarker in preparation of a prognosis evaluation product of NPM1 mutation AML. The evaluation product is a kit, and the kit comprises an OPTN gene probe or an anti-OPTN antibody. The kit is used for quantifying the OPTN mRNA or protein level in a bone marrow sample. According to the scheme provided by the invention, the OPTN is highly expressed in the NPM1 mutation AML and is related to poor prognosis, so that the OPTN becomes an ideal treatment target; by inhibiting the OPTN, the mitochondrial autophagy pathway can be accurately blocked, the drug resistance of leukemia cells is reduced, and normal cells are not influenced.

The embodiment of the invention provides a gene panel, a probe, a kit and a method for detecting bony mandibular prolapse, and belongs to the technical field of gene detection. The gene panel for detecting the bony mandibular prolapse comprises a plurality of target genes. According to the scheme, the reported bony mandibular preprocess related genes and the potential core pathogenic genes screened through the network propagation algorithm are integrated at the same time, a genetic detection panel which is wider in coverage and higher in relevance is constructed, and systematic capture and high-confidence identification of the bony mandibular preprocess pathogenic genes are achieved. The panel can detect functional mutation of multiple gene loci at a time, the problem that key genetic factors are omitted in traditional single-gene detection is solved, and the detection sensitivity and specificity are improved.

The embodiment of the invention belongs to the technical field of detection, and relates to a kit for detecting influenza B virus and application, the kit comprises a primer and a probe, the nucleotide sequence of an upstream primer of a flow B is as shown in SEQ ID NO: 1, the nucleotide sequence of a downstream primer of the flow B is as shown in SEQ ID NO: 2, the nucleotide sequence of the flow B probe is as shown in SEQ ID NO: 5, and the nucleotide sequence of the flow B probe is as shown in SEQ ID NO: 2. The nucleotide sequence of the upstream primer of the internal control gene is shown as SEQ ID NO: 3, the nucleotide sequence of the downstream primer of the internal control gene is shown as SEQ ID NO: 4, the nucleotide sequence of the internal control gene probe is shown as SEQ ID NO: 6, and the kit is further applied to qualitative and quantitative detection of suspected influenza B virus nucleic acid fragments. According to the application, the requirement on quantitative detection of the influenza B virus is met.

The invention provides a FISH probe combination and kit for detecting NECTIN4 gene amplification and a preparation method and application of the FISH probe combination and kit. The FISH probe combination comprises an NECTIN4 gene probe and a first chromosome internal reference probe; a preparation template of the NECTIN4 gene probe is composed of any one of the following BAC clone plasmids (1) to (3): (1) the BAC clone plasmids are RP11-157H6, CTD-3003O12 and RP11-4N6, and (2) the BAC clone plasmids are RP11-157H6, CTD-3003O12 and RP11-4N6; (2) cloning plasmids CTD-2299J15 and CTD-2502B20 by using a BAC (Bacterial Amplification Cellulose); and (3) cloning the plasmid CTD-2502B20 by using the BAC. The probe combination can detect the amplification condition of the NECTIN4 gene of a patient, provides data support for clinical tests and individualized treatment, has the characteristics of high sensitivity and good specificity, and can rapidly hybridize within 1 hour and stably interpret the signal intensity at high energy.