54 results about "Major gene" patented technology

A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention relates to the technical field of molecular breeding and phytopathology, in particular to a KASP molecular marker of a wheat stripe rust resistant gene and application of the KASP molecular marker. The invention particularly relates to a functional SNP (Single Nucleotide Polymorphism) marker of a wheat stripe rust resistance major gene Chr6B excavated on the basis of Meta-GWAS (Complete Genome Association Analysis), and application of a KASP (Kinase Amplified Sequence Protein) molecular marker system developed on the basis of an SNP locus in wheat disease-resistant germplasm resource screening, molecular marker-assisted breeding and gene pyramiding breeding. The major effect of the MQTL6B.2 site on the Chr6B chromosome in resistance phenotypic variation is disclosed for the first time, and the contribution rate is greater than or equal to 28.54%. The primer can distinguish a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.

The invention provides a molecular marker primer closely linked with a brown planthopper resistant major gene Bph48 of rice as well as a marking method and application of the molecular marker primer. A rice insect-resistant variety 75-9-3 (called C268 for short) and Yangdao 9 (called 9311 for short) are hybridized to obtain genotypes of F2 individual plants, genetic linkage analysis is performed in combination with the brown planthopper resistance level of an F3 family, a resistance major gene Bph48 carried by the insect-resistant variety C268 is identified, a Bph48 positioning region is reduced to a 370kb fragment limited by markers 1M30.093 and 1M30.471 on the two sides, and the Bph48 gene is identified to be a Bph48 gene. And the molecular marker is closely linked with the molecular marker 1M30.471. The molecular marker 1M30.471 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C268 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph48 is obtained.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of the corrected 100 kg day age of a pig and application, major genes and functional mutation related to the corrected 100 kg day age of the pig are obtained through screening and are verified in a group, and a new molecular marker is provided for auxiliary selection and prediction improvement of the corrected 100 kg day age of the pig. The method has important significance on auxiliary screening of pigs.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph33 of a rice variety W41123 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker KBPH33 which is closely linked with a rice brown planthopper resistant gene locus Bph33 and has good amplification efficiency. The marker detection is located at the 19571017 site of the No.4 chromosome of Nipponbare rice, the polymorphism is A / G, and a marker primer developed on the basis of a KASP technology is as shown in SEQ ID No: 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph33 site, can be conveniently and efficiently used for efficiently identifying whether W41123 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper in a period, and greatly improves the breeding efficiency.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The application discloses an InDel molecular marker related to peanut crown rot resistance and application, and belongs to the field of molecular biology.The polymorphism of the InDel molecular marker on an A01 chromosome is that a 177 bp nucleotide sequence exists or is lost, and the nucleotide sequence is shown as SEQ ID NO.5.The molecular marker is based on a resistance major gene qCRA01 The co-dominant marker developed from the candidate gene is closely related to the resistance trait, and rapid resistance identification and screening of peanut breeding materials and germplasm resources can be realized.The application provides a reliable tool for peanut crown rot resistance molecular marker assisted breeding. Early selection is carried out by using the molecular marker, the workload of field identification can be greatly reduced, the introduction and aggregation of disease resistance genes to excellent backgrounds can be accelerated, the breeding period is shortened, and the breeding efficiency is improved, and therefore the application has important application value for cultivating high-yield, high-quality and disease-resistant peanut new varieties.

The invention provides an application of a CsPPC3 gene in regulation and control of carbon and nitrogen metabolism of cucumber fruits, a major gene CsPPC3 for coding phosphoenolpyruvate carboxylase (PEPC) in carbon fixation of the cucumber fruits is taken as a research target, an overexpression and gene knockout transgenic plant is constructed, the effect of the CsPPC3 gene in carbon fixation and carbon and nitrogen metabolism of photosynthesis of the fruits is researched, and the CsPPC3 gene is applied to regulation and control of carbon and nitrogen metabolism of the cucumber fruits. It is found that the amount of CO2 released by respiration of a transgenic plant overexpressed by the CsPPC3 gene is reduced, the CO2 refixation capacity is enhanced, the single fruit weight of the fruit is increased, the content of malic acid, soluble sugar and starch is increased, and the content of amino acid is reduced, while the amount of CO2 released by respiration of a transgenic plant knocked out by the CsPPC3 gene is increased, and the single fruit weight of the fruit is reduced. Meanwhile, the contents of malic acid, soluble sugar and amino acid are reduced, the starch content is increased, and the CO2 refixing capacity is reduced. The method disclosed by the invention has practical guiding significance for cultivating transgenic plants with high CO2 fixing efficiency.

The present application discloses a multiplex KASP marker primer set for major genes controlling wheat plant height, which consists of a forward primer with a nucleotide sequence as shown in SEQ ID NO.10, a reverse primer with a nucleotide sequence as shown in SEQ ID NO.11, and a common primer with a nucleotide sequence as shown in SEQ ID NO.9; this multiplex KASP marker primer set can simultaneously detect the Rht-B1 and Rht-D1 genes in wheat; compared with the existing detection methods, this primer set realizes the simultaneous detection of the Rht-B1 and Rht-D1 genes for the first time, doubles the efficiency, reduces the cost by half, greatly improves the breeding efficiency, and has broad application prospects.

The invention relates to the technical field of molecular genetics, in particular to a molecular marker primer of a winter melon fruit type major gene BhFS2.1 as well as a marking method and application of the molecular marker primer. According to the molecular marker primer, an F2 genetic population is obtained by hybridizing and selfing a long and thin white gourd material and a thick and short white gourd material, and then QTL detection is performed by combining the genotype and phenotype of the F2 genetic population; a major gene for controlling the fruit type of the winter melon is successfully identified and named as BhFS2.1, a molecular marker closely linked with the gene is developed, two pairs of simple and easy-to-use functional markers 2M57.86 and 2M59.91 are obtained, and experimental verification shows that the two molecular markers can accurately judge whether a breeding intermediate material is a slender fruit type wax gourd or a thick and short fruit type wax gourd in the seedling stage, so that the breeding intermediate material can be quickly and accurately selected. The method can be used for quickly screening required fruit type strains (species) in the seedling stage, and the detection is convenient, quick and convenient and is not influenced by the environment.

Recombinant adeno-associated virus (AAV) vectors are a major gene delivery platform, and the clinical use of several AAV-mediated therapies has recently been approved. Disclosed herein are compositions comprising AAV capsid proteins exhibiting improved tropism and improved transduction efficiency to renal cells and kidney-associated cells, as well as methods for using AAV particles and AAV vectors containing these AAV capsid proteins to efficiently deliver a gene or transgene of interest to target cells or tissues, and to treat subjects requiring treatment.

This invention provides a molecular marker primer tightly linked to the major brown planthopper resistance gene Bph57 in rice, along with its marking method and application. This invention utilizes genetic linkage analysis of individual F2 plants obtained from crossing the insect-resistant rice variety “Daomushe Late Bengu” (C9) (♂) with 9311 (♀), combined with the brown planthopper resistance levels of F3 families. This analysis identifies the major resistance gene Bph57 carried by the insect-resistant variety C9. The Bph57 region is narrowed down to an approximately 90kb fragment defined by flanking markers 2M26.442 and 2M26.536, and tightly linked to molecular marker 2M26.762. The molecular marker 2M26.762 of this invention effectively detects whether the insect-resistant variety C9 and its derived varieties (lines) contain this major resistance gene locus, significantly improving the selection efficiency for brown planthopper resistance and obtaining brown planthopper-resistant rice varieties (lines) containing Bph57.

The application discloses a KASP molecular marker closely linked to a BNS series of wheat male sterility major gene site and application thereof, the BNS series of wheat male sterility major gene site is located on a wheat 2A chromosome, and the KASP molecular marker closely linked to the BNS series of wheat male sterility major gene site is AX-174216228 and AX-109413735; two KASP markers AX-174216228 and AX-109413735 provided by the application are used to verify 97 wheat materials, and the result is completely consistent with expectation. The KASP marker provided by the application has the characteristics of rapidness, convenience, high specificity and good accuracy, and can complete screening and phenotype prediction work of the male sterility major gene site in a large number of materials in a short time.

The invention discloses an InDel molecular marker InDel-P1 closely associated with barley salt tolerance and application of the InDel molecular marker InDel-P1, and belongs to the technical field of crop molecular marker assisted breeding. The invention develops an InDel molecular marker InDel-P1 which is closely associated with a salt-tolerant major gene located on a barley 2H chromosome. Through group salt tolerance correlation analysis, it is proved that the InDel-P1 marker is remarkably related to salt tolerance, the correlation coefficient is 0.63, and the InDel-P1 marker can be effectively used for screening barley salt-tolerant varieties. The molecular marker is detected by 4% agarose gel, the method is simple, the result is reliable, the selection efficiency of salt-tolerant germplasm and salt-tolerant single plant is improved, and the breeding process of salt-tolerant barley varieties is accelerated.

The invention discloses a genome optimization configuration analysis system and method for molecular marker-assisted breeding, and relates to the technical field of biological information. The method is used for solving the problems of genetic interaction distortion, environmental response lag and breeding execution disjunction in multi-gene collaborative optimization. The system comprises a conflict resolution module, an environment adaptation module, an interaction compression module and a configuration generation module, wherein the conflict resolution module calculates and corrects an interaction effect value through phenotypic deviation and environment fluctuation, generates a linkage burdens intensity index and dynamically allocates a character weight; the environment adaptation module is used for fusing the stress data and a historical threshold value to identify a stress period and lock a key gene interval; the interaction compression module constructs a radial interaction network taking a major gene as a core and generates a linkage vector; and the configuration generation module analyzes key vector dimensions, locates recombination hotspots, matches complementary parents and outputs a structured breeding instruction, so that a breeding optimization closed loop from interaction recognition to parent combination recommendation is realized.

The present invention discloses a SNP molecular marker for watermelon fruit size genes and its application, aiming to solve the technical problem of lacking recognition and identification methods for the major genes controlling watermelon fruit size. The present invention provides a SNP locus of a watermelon fruit size gene (the base at position 27,105,097 of chromosome 8 of the watermelon 97103 reference genome version 2 is mutated from T to C), and designed CAPS molecular marker primers v2ft36 based on the enzyme digestion information of the SNP locus. Applying this marker for molecular marker-assisted selection breeding can more quickly and accurately conduct directional genetic improvement of watermelon fruit size; the fruit size of watermelon can be quickly identified through simple experimental operations and analyses, providing a rapid, simple, scientific and practical detection and identification technology for the detection of watermelon fruit size traits.

The application provides cloning of a soybean oil content regulating gene and its encoded protein and application, and relates to the fields of plant molecular genetics and genetic engineering. The soybean oil content regulating gene is qOil10, and the nucleotide sequence is shown in the sequence table SEQ ID NO:1. A major gene controlling soybean oil content is successfully cloned, the function of the gene in positively regulating seed oil accumulation is determined, and the shortage of insufficient soybean oil functional gene resources is filled. The application further provides gene positioning primers, knockout primers, recombination vectors and genetic transformation methods, which can be directly applied to molecular marker assisted selection breeding, significantly improve the breeding efficiency of high-oil soybeans, and shorten the breeding cycle. Compared with conventional breeding, the application is precise in trait improvement and simple in operation, and provides key technical support for cultivating new high-oil soybean varieties and ensuring oil supply safety.

The application provides a primer set of a molecular marker closely linked to a major sterile locus of indica-japonica subspecies hybridization, including a primer set of kasp2-14 and a primer set of kasp2-15, the primer set of kasp2-14 includes kasp2-14 forward primer 1, kasp2-14 forward primer 2 and kasp2-14 common reverse primer, and the nucleotide sequences are SEQ ID NO. 1-3 in sequence, the primer set of kasp2-15 includes kasp2-15 forward primer 1, kasp2-15 forward primer 2 and kasp2-15 common reverse primer, and the nucleotide sequences are SEQ ID NO. 4-6 in sequence, and a detection method is also provided, and the primer set is applied to identification of a sterile locus S69 of indica-japonica subspecies hybridization. The primer set can improve the accuracy and efficiency of detection of the sterile locus S69 in indica-japonica subspecies hybridization combinations.

The invention discloses KASP molecular markers closely linked with BNS series wheat male sterility major gene loci and application of the KASP molecular markers, the BNS series wheat male sterility major gene loci are located on a wheat 2A chromosome, and the KASP molecular markers closely linked with the BNS series wheat male sterility major gene loci are AX-174216228 and AX-109413735; the two KASP markers AX-174216228 and AX-109413735 provided by the invention are used for verifying 97 parts of wheat materials, and the results of the two KASP markers AX-174216228 and AX-109413735 completely meet the expectation. The KASP marker provided by the invention has the characteristics of rapidness, simplicity, convenience, high specificity, good accuracy and the like, and the screening and phenotype prediction work of the male sterility major gene locus in a large batch of materials can be completed in a relatively short time.

The invention relates to cloning of a rice chalkiness phenotype average value and plasticity major gene MPC5 and application of the rice chalkiness phenotype average value and plasticity major gene MPC5 in quality improvement. The major gene MPC5 capable of regulating and controlling the average value and plasticity of chalkiness phenotypes in an opposite mode is determined, and the verification of transgenic tests in a multi-point high-temperature and normal-temperature field for many years shows that after the MPC5 is knocked out, the chalkiness degree of grains is remarkably increased while the grain shape is remarkably widened, and the plasticity of the grains is reduced; overexpressed MPC5 grains are remarkably narrowed, the chalkiness is remarkably reduced, the plasticity is improved, and the expression level of the MPC5 is remarkably improved under a high-temperature condition. The major QTL for controlling the chalkiness phenotype average value and plasticity, the molecular breeding minimum SNPs combination of the major QTL and the molecular breeding minimum SNPs combination and the cloned major gene MPC5 for controlling the chalkiness phenotype average value and plasticity are important targets for cultivating high-temperature-resistant high-quality rice varieties and have wide application value.

The application relates to a cotton fiber strength major gene NTL9 BS41 and application thereof. Through cotton transgenic technology, NTL9 BS41 is successfully introduced into Gossypium hirsutum and high expression of the gene in cotton fibers is realized, the fiber strength of cotton is significantly enhanced without negative influence on other fiber qualities, overexpression of NTL9 BS41 can effectively enhance the density and uniformity of cellulose microfibril accumulation, and the structure and performance of cotton fibers are significantly optimized. In addition, the gene is derived from Gossypium barbadense, compared with Gossypium hirsutum, there is an insertion type variation of 24 bp nucleotides in the CDS of the gene, and a molecular marker capable of effectively distinguishing Gossypium hirsutum and Gossypium barbadense haplotypes is successfully developed. The application provides new gene resources, technical means and germplasm resources for cotton fiber quality improvement, and has far-reaching application value and potential for promoting the progress of cotton breeding work.

The disclosure discloses a method and application for rapid and accurate chromosomal location of economic traits in laver, belonging to the fields of genomics and molecular breeding, wherein comprising the following steps: distinguishing the different genotype sectors by the color difference of pigment mutants, releasing monospores based on the asexual reproduction of single-genotype sectors, forming offspring CMD population; performing QTL-seq analysis on the extreme phenotype pools of offspring CMD population by SNP / InDel markers; and combining KASP and RNA-seq to predict the location of the genes of discrete traits or major QTL. The disclosure may solve the problem of difficulty in genetic analysis of various traits caused by the genotypic chimeric haploid characteristics.

The invention relates to a DNA (Deoxyribose Nucleic Acid) of a gene OsMKKK32 for regulating and controlling rice grain shape, thousand grain weight and salt tolerance and breeding utilization of a protein of the gene OsMKKK32. The grain width major gene OsMKKK32 is cloned by performing QTL (quantitative trait loci) analysis on the grain width character of an RIL group constructed by backcrossing the GXB (GXB) and the Dular for many years and combining quantitative analysis and gene annotation. By adopting a gene editing and molecular marker assisted breeding technology, an OsMKKK32 knockout near-isogenic line material and a near-isogenic line material with different haplotypes are obtained. The OsMKKK32 is knocked out or the expression of the OsMKKK32 is reduced, so that the grain width, the grain thickness and the thousand grain weight of rice grains are remarkably reduced, and the grain length is remarkably increased. Salt tolerance analysis shows that the expression of the OsMKKK32 gene is inhibited by salt, and the salt tolerance of the rice is remarkably improved by knocking out the OsMKKK32. The OsMKKK32 different haplotype near-isogenic line material provides material and technical support for high yield of rice and cultivation of saline-alkaline tolerant rice.

The invention relates to a breeding method of a fluoride-resistant and polyhedrosis-resistant bombyx mori strain, and belongs to the technical field of bombyx mori genetic breeding. In order to solve the technical problems that an existing silkworm variety cannot resist fluoride poisoning and pyosis at the same time, and resistance is prone to loss, the method comprises the following steps: hybridizing a germplasm carrying a fluorine-resistant major gene and a germplasm carrying an antiviral dominant major gene to obtain an F1 generation, and backcrossing the F1 generation with a fluorine-resistant parent after fluorine-resistant screening to obtain a BC1 generation; and finally, fluorine-resistant and antiviral characters are synchronously screened and improved through selfing generations, and directional breeding is carried out by combining whole-course recorded development and cocoon quality characters. According to the method, a new silkworm strain with double resistance is successfully bred, and the method is mainly used for solving the problem of low survival rate of silkworm breeding in a fluorine pollution area and a virus high incidence area in industrial production.

The invention provides a probe set for detecting genetic chronic granulomatosis related gene mutation, a kit and application, and relates to the technical field of molecular biology. The hereditary chronic granulomatosis related genes comprise a CYBA gene, an NCF1 gene, an NCF2 gene, a CYBB gene, a WG gene, an NCF4 gene, a CYBC1 gene, an ASAH1 gene, an RAG2 gene and an RAG1 gene. Through a targeted panel customized by the probe, major genes related to chronic granuloma can be efficiently captured, the detection efficiency and the positive rate can be greatly improved, the period is shortened, and the detection cost of genetic CGD second-generation high-throughput sequencing is reduced.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph36 (t) of a rice variety PSL2 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker C9100ZL which is closely linked with a rice brown planthopper resistant gene locus Bph36 (t) and has good amplification efficiency. The marker detection is located at the 1434111 site of the shuhui498 rice chromosome 6, the polymorphism is C / T, and a marker primer developed based on the KASP technology is shown as SEQ ID No 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph36 (t) site, can be conveniently and efficiently used for identifying whether PSL2 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper, and greatly improves the breeding efficiency.

The invention belongs to the field of molecular breeding, and particularly relates to a group of QTLs (quantitative trait loci) related to wheat grain roundness as well as KASP primers and application of the QTLs. According to the specific technical scheme, the major gene locus QGr.cib-5A related to the wheat grain roundness is located on a short arm of a wheat 5A chromosome. By utilizing the QTL and the corresponding KASP molecular marker found by the invention, early generation detection can be carried out on the grain roundness in wheat breeding, the workload is reduced, the breeding period is shortened, the breeding efficiency is improved, and the application prospect is good.

The present invention relates to a biomarker for predicting a treatment response to immunotherapy and a gene prediction model using the same. The gene prediction model using the biomarker is based on the expression pattern of a main gene by which characteristics of a tumor microenvironment of a patient can be recognized, and thus can predict a treatment response. Therefore, the gene prediction model makes it possible to avoid unnecessary surgery and determine an optimal treatment plan.