The invention discloses a structure and a
major gene locus Psr9 of a pod shattering resistance character of a rape and application thereof. A preparation method of the
major gene locus Psr9 of the pod shattering resistance character of the rape comprises the following steps of: (a) hybridizing by utilizing a high-low pod shattering resistance
assembly zy72360 of the rape and R1, and carrying out
selfing on an F1 generation to generate an F2 generation segregation
population; (b) extracting a parent zy72360 and R1, the leaf total
DNA of the F1 generation and F2 generation segregation
population; (c) collecting rape SSR (Simple
Sequence Repeat)
label public databases, autonomously developing an SSR
label, and screening polymorphic primers by utilizing the parent zy72360; (d) establishing a genetic map through distributions inside the F2 generation segregation
population, carrying out QTL (Quantitative Trait Loci) locus analysis by dint of pod shattering resistance index data, and obtaining the SSR
label closely concatenated with a
major gene of the pod shattering resistance character; and (e) observing the parent zy72360 and the structure of a mature pod of the F2 generation segregation population through a dissecting
microscope, and measuring the combining area index of a carpel and a
placenta frame. The invention has the advantages of enhanced selection efficiency, definite position of the pod shattering resistance major
gene locus Psr9, convenience and fastness for detection, fast screening of a high pod shattering resistance strain used for the pod shattering resistance breeding of the rape, definite breeding selection target and cost saving.