27 results about "Major gene" patented technology

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention provides a molecular marker primer closely linked with a brown planthopper resistant major gene Bph48 of rice as well as a marking method and application of the molecular marker primer. A rice insect-resistant variety 75-9-3 (called C268 for short) and Yangdao 9 (called 9311 for short) are hybridized to obtain genotypes of F2 individual plants, genetic linkage analysis is performed in combination with the brown planthopper resistance level of an F3 family, a resistance major gene Bph48 carried by the insect-resistant variety C268 is identified, a Bph48 positioning region is reduced to a 370kb fragment limited by markers 1M30.093 and 1M30.471 on the two sides, and the Bph48 gene is identified to be a Bph48 gene. And the molecular marker is closely linked with the molecular marker 1M30.471. The molecular marker 1M30.471 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C268 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph48 is obtained.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph33 of a rice variety W41123 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker KBPH33 which is closely linked with a rice brown planthopper resistant gene locus Bph33 and has good amplification efficiency. The marker detection is located at the 19571017 site of the No.4 chromosome of Nipponbare rice, the polymorphism is A / G, and a marker primer developed on the basis of a KASP technology is as shown in SEQ ID No: 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph33 site, can be conveniently and efficiently used for efficiently identifying whether W41123 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper in a period, and greatly improves the breeding efficiency.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The application discloses an InDel molecular marker related to peanut crown rot resistance and application, and belongs to the field of molecular biology.The polymorphism of the InDel molecular marker on an A01 chromosome is that a 177 bp nucleotide sequence exists or is lost, and the nucleotide sequence is shown as SEQ ID NO.5.The molecular marker is based on a resistance major gene qCRA01 The co-dominant marker developed from the candidate gene is closely related to the resistance trait, and rapid resistance identification and screening of peanut breeding materials and germplasm resources can be realized.The application provides a reliable tool for peanut crown rot resistance molecular marker assisted breeding. Early selection is carried out by using the molecular marker, the workload of field identification can be greatly reduced, the introduction and aggregation of disease resistance genes to excellent backgrounds can be accelerated, the breeding period is shortened, and the breeding efficiency is improved, and therefore the application has important application value for cultivating high-yield, high-quality and disease-resistant peanut new varieties.

Recombinant adeno-associated virus (AAV) vectors are a major gene delivery platform, and the clinical use of several AAV-mediated therapies has recently been approved. Disclosed herein are compositions comprising AAV capsid proteins exhibiting improved tropism and improved transduction efficiency to renal cells and kidney-associated cells, as well as methods for using AAV particles and AAV vectors containing these AAV capsid proteins to efficiently deliver a gene or transgene of interest to target cells or tissues, and to treat subjects requiring treatment.

This invention provides a molecular marker primer tightly linked to the major brown planthopper resistance gene Bph57 in rice, along with its marking method and application. This invention utilizes genetic linkage analysis of individual F2 plants obtained from crossing the insect-resistant rice variety “Daomushe Late Bengu” (C9) (♂) with 9311 (♀), combined with the brown planthopper resistance levels of F3 families. This analysis identifies the major resistance gene Bph57 carried by the insect-resistant variety C9. The Bph57 region is narrowed down to an approximately 90kb fragment defined by flanking markers 2M26.442 and 2M26.536, and tightly linked to molecular marker 2M26.762. The molecular marker 2M26.762 of this invention effectively detects whether the insect-resistant variety C9 and its derived varieties (lines) contain this major resistance gene locus, significantly improving the selection efficiency for brown planthopper resistance and obtaining brown planthopper-resistant rice varieties (lines) containing Bph57.

The invention discloses a genome optimization configuration analysis system and method for molecular marker-assisted breeding, and relates to the technical field of biological information. The method is used for solving the problems of genetic interaction distortion, environmental response lag and breeding execution disjunction in multi-gene collaborative optimization. The system comprises a conflict resolution module, an environment adaptation module, an interaction compression module and a configuration generation module, wherein the conflict resolution module calculates and corrects an interaction effect value through phenotypic deviation and environment fluctuation, generates a linkage burdens intensity index and dynamically allocates a character weight; the environment adaptation module is used for fusing the stress data and a historical threshold value to identify a stress period and lock a key gene interval; the interaction compression module constructs a radial interaction network taking a major gene as a core and generates a linkage vector; and the configuration generation module analyzes key vector dimensions, locates recombination hotspots, matches complementary parents and outputs a structured breeding instruction, so that a breeding optimization closed loop from interaction recognition to parent combination recommendation is realized.

The application provides cloning of a soybean oil content regulating gene and its encoded protein and application, and relates to the fields of plant molecular genetics and genetic engineering. The soybean oil content regulating gene is qOil10, and the nucleotide sequence is shown in the sequence table SEQ ID NO:1. A major gene controlling soybean oil content is successfully cloned, the function of the gene in positively regulating seed oil accumulation is determined, and the shortage of insufficient soybean oil functional gene resources is filled. The application further provides gene positioning primers, knockout primers, recombination vectors and genetic transformation methods, which can be directly applied to molecular marker assisted selection breeding, significantly improve the breeding efficiency of high-oil soybeans, and shorten the breeding cycle. Compared with conventional breeding, the application is precise in trait improvement and simple in operation, and provides key technical support for cultivating new high-oil soybean varieties and ensuring oil supply safety.

The application provides a primer set of a molecular marker closely linked to a major sterile locus of indica-japonica subspecies hybridization, including a primer set of kasp2-14 and a primer set of kasp2-15, the primer set of kasp2-14 includes kasp2-14 forward primer 1, kasp2-14 forward primer 2 and kasp2-14 common reverse primer, and the nucleotide sequences are SEQ ID NO. 1-3 in sequence, the primer set of kasp2-15 includes kasp2-15 forward primer 1, kasp2-15 forward primer 2 and kasp2-15 common reverse primer, and the nucleotide sequences are SEQ ID NO. 4-6 in sequence, and a detection method is also provided, and the primer set is applied to identification of a sterile locus S69 of indica-japonica subspecies hybridization. The primer set can improve the accuracy and efficiency of detection of the sterile locus S69 in indica-japonica subspecies hybridization combinations.

The application relates to a cotton fiber strength major gene NTL9 BS41 and application thereof. Through cotton transgenic technology, NTL9 BS41 is successfully introduced into Gossypium hirsutum and high expression of the gene in cotton fibers is realized, the fiber strength of cotton is significantly enhanced without negative influence on other fiber qualities, overexpression of NTL9 BS41 can effectively enhance the density and uniformity of cellulose microfibril accumulation, and the structure and performance of cotton fibers are significantly optimized. In addition, the gene is derived from Gossypium barbadense, compared with Gossypium hirsutum, there is an insertion type variation of 24 bp nucleotides in the CDS of the gene, and a molecular marker capable of effectively distinguishing Gossypium hirsutum and Gossypium barbadense haplotypes is successfully developed. The application provides new gene resources, technical means and germplasm resources for cotton fiber quality improvement, and has far-reaching application value and potential for promoting the progress of cotton breeding work.

The invention relates to a breeding method of a fluoride-resistant and polyhedrosis-resistant bombyx mori strain, and belongs to the technical field of bombyx mori genetic breeding. In order to solve the technical problems that an existing silkworm variety cannot resist fluoride poisoning and pyosis at the same time, and resistance is prone to loss, the method comprises the following steps: hybridizing a germplasm carrying a fluorine-resistant major gene and a germplasm carrying an antiviral dominant major gene to obtain an F1 generation, and backcrossing the F1 generation with a fluorine-resistant parent after fluorine-resistant screening to obtain a BC1 generation; and finally, fluorine-resistant and antiviral characters are synchronously screened and improved through selfing generations, and directional breeding is carried out by combining whole-course recorded development and cocoon quality characters. According to the method, a new silkworm strain with double resistance is successfully bred, and the method is mainly used for solving the problem of low survival rate of silkworm breeding in a fluorine pollution area and a virus high incidence area in industrial production.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an anti-brown planthopper major gene locus Bph36 (t) of a rice variety PSL2 and application of the SNP molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) marker C9100ZL which is closely linked with a rice brown planthopper resistant gene locus Bph36 (t) and has good amplification efficiency. The marker detection is located at the 1434111 site of the shuhui498 rice chromosome 6, the polymorphism is C / T, and a marker primer developed based on the KASP technology is shown as SEQ ID No 1-3. The SNP marker disclosed by the invention can be used for detecting a close linkage site of a Bph36 (t) site, can be conveniently and efficiently used for identifying whether PSL2 and derived varieties thereof contain the major gene site or not, can predict the resistance level of brown planthopper, and greatly improves the breeding efficiency.

The invention discloses identification of a molecular marker associated with low-temperature resistance of rape and application of the molecular marker, a major gene locus of an oil content character located in a C06 chromosome of the rape is screened out, and two molecular markers LTRSWU6C06M1 and LTRSWU6C06M2 which are closely linked with the major gene locus are developed. Whether an SWU6 derivative line contains the major QTL site or not is detected through the two molecular markers, then a low-temperature resistant line is rapidly screened for rape breeding, the assistant breeding selection target is clear, and the cost is saved.

This invention relates to the field of plant genetic engineering technology, specifically disclosing a major gene for cold tolerance in rice seedlings, its encoded protein, and its applications. The major gene... RGA4L The nucleotide sequence is shown in SEQ ID No:1. This invention relates to a major gene controlling cold tolerance in rice seedlings. RGA4L After the expression of the gene was reduced in rice, the T3 transgenic plants showed a significant decrease in cold tolerance and became cold-sensitive; the gene was expressed or overexpressed. RGA4L Rice varieties with this gene exhibit high survival rates at low temperatures and demonstrate cold tolerance. Research on the molecular mechanism regulating cold tolerance in rice seedlings has significant theoretical and practical implications for the breeding of new cold-tolerant rice varieties. It also has broad application potential and market prospects in the agricultural field.

The invention relates to the field of plant genetic engineering, in particular to application of an MYB transcription factor gene HvPHL2 in regulating and controlling the content of beta-glucan in barley grains. The MYB transcription factor gene HvPHL2 participating in regulation and control of the barley grain beta-glucan content is found, and through construction experiments of yeast single impurity, gel migration, dual luciferase and overexpression genetic materials, it is proved that the transcription factor HvPHL2 can be combined to CCAAT-box of a promoter region of a barley grain beta-glucan synthesis major gene HvCslf6, expression of an HvCslf6 promoter is inhibited, and the content of the barley grain beta-glucan in the barley grain beta-glucan synthesis major gene HvCslf6 in the barley grain beta-glucan synthesis major gene HvCslf6 in the barley grain beta-glucan synthesis major gene HvCslf6 is regulated and controlled. The method plays a key role in regulation and control of beta-glucan synthesis, and provides a new theoretical basis for genetic regulation and control of the beta-glucan content of the barley grains.

The invention provides a close-linkage molecular marker primer of a rice brown planthopper-resistant major gene Bph55 as well as a marking method and application of the close-linkage molecular marker primer. According to genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety QG44 (sex) and 9311 (sex), genetic linkage analysis is performed in combination with the resistance level of an F3 family to brown planthopper; a resistance major gene Bph55 carried by an insect-resistant variety QG44 is identified, and a Bph55 region is shrunk to a 270kb fragment limited by markers 7M10.726 and 7M10.996 on the two sides and is closely linked with a molecular marker 7M10.726. The molecular marker 7M10.726 provided by the invention can effectively detect whether an insect-resistant variety QG44 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph55 is obtained.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhNAC2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The invention discloses a major site qSOCA08-1 for regulating and controlling the oil content of peanut seed kernels, and the phenotypic variation of 11.41-20.97% is explained. The qSOCA08-1 is finely positioned in a 352.2 kb physical interval between a No.8 chromosome marker Tif2. A08.38582892 and a No.8 chromosome marker Tif2. A08.38935104, and a candidate gene of the qSOCA08-1 is confirmed to be AhNAC2. The invention also discloses a method for identifying the qSOCA08-1. The 285th basic group of the coding sequence of the AhNAC2 shows C-A nonsense mutation between parents Jihuansweet No.1 and W191, and the early termination can cause the function loss of the AhNAC2. A competitive allele specific PCR molecular marker is developed on the basis of a C-A mutation SNP site, the association between the molecular marker and the oil content of the seed kernel is successfully verified in a peanut germplasm resource material and a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

The invention discloses a multiple PCR (Polymerase Chain Reaction) Panel for detecting favorable allelic variation of a wheat gibberellic disease resistant site and application of the multiple PCR Panel. The Panel takes a gibberellic disease resistant major gene Fhb1 and important resistance sites QFhb5A and QFhb2D derived from backbone parents in the middle and lower reaches of the Yangtze River as targets, can synchronously and accurately detect multi-site allelic variation in a single-tube reaction, and has the advantages of high throughput, low cost, simplicity and convenience in operation, high specificity and the like. The invention further establishes a wheat gibberellic disease-resistant molecular design breeding strategy based on the Panel, forms a cooperative selection mode of'precise parent matching + multi-site targeted detection + comprehensive character evaluation ', and realizes rapid polymerization and efficient fixation of multi-site favorable allelic variation. In a word, the multi-PCR Panel and a matched molecular breeding strategy of the multi-PCR Panel are favorable for improving the targeted selection efficiency and accuracy of fusarium head blight resistance pyramiding breeding of the wheat, and a feasible technical means is provided for cultivating new wheat germplasm with stable resistance and coordinated comprehensive characters.

The invention relates to the technical field of gene detection, and discloses a multiple PCR primer structure for detecting solid tumor gene fusion and a method for constructing an NGS library, the multiple PCR primer structure for detecting solid tumor gene fusion and the method for constructing the NGS library comprise the following steps: S1, linker connection; s2, purification after connection; s3, carrying out specific amplification; s4, purifying after specific amplification: purifying a PCR amplification product obtained in S3 to obtain a purified NGS library; s5, sequencing on a machine and analyzing a result. According to the multiple PCR primer structure for detecting solid tumor gene fusion and the method for constructing the NGS library, an omega type target primer is designed for a main gene exon participating in fusion, so that all gene sequences fused by the exon can be amplified without difference while the specificity is greatly improved, and the purpose of detecting unknown gene fusion of partners is achieved; the method is simple to operate, short in time consumption, low in cost, high in specificity and capable of simultaneously detecting fusion of various genes.

The invention relates to the technical field of molecular biology, in particular to a method for detecting the genotype of a sheep multi-fetal major gene FecB based on an isothermal amplification technology. The invention provides the specific RPA primer group for the sheep multi-fetal major gene FecB, and a novel method for typing the polymorphic sites of the sheep multi-fetal major gene FecB is established by utilizing an RPA technology, and the method has the advantages of simplicity in operation, high efficiency, rapidness and high accuracy; genetic typing of the sheep multi-fetal major gene FecB and identification of sheep multi-fetal character individuals can be rapidly achieved on a large scale, a pasture is helped to conduct early selection on high-reproduction breeding stock, the group reproduction efficiency can be effectively improved, genetic improvement of the lambing number, the lambing rate and other characters of a sheep flock can be accelerated, continuous optimization of the population is achieved, and the method has wide application prospects.

The invention belongs to the field of molecular biology, provides a functional molecular marker of a major gene AhARF2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker, discloses a major site qSOCA08-3 for regulating and controlling the oil content of the peanut seed kernels, and explains phenotypic variation of 14.91%-17.31%. The qSOCA08-3 is finely positioned to a physical interval of 349.6 kb between the Tif2. A08.49681404 and the Tif2. A08.50031016, and a candidate gene of the qSOCA08-3 is confirmed to be AhARF2. The qSOCA08-3 has the advantages that the qSOCA08-3 can be used for identifying the The AhARF2 coding sequence shows 275bp deletion between parents Jianhua No.1 and W191 and is accompanied by 7bp replacement, so that the code shift mutation of the coding sequence is caused, and the protein translation is terminated in advance. According to the invention, the KASP molecular marker is developed on the basis of a mutant sequence, the association between the KASP molecular marker and the oil content of the seed kernel is successfully verified in a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in the marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhWRI1 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The molecular marker is located at InDel sites of 45bp, 716bp, 738bp-45bp, 718bp and 423bp of a No.8 chromosome of peanuts, the sequence of the molecular marker is SEQ ID NO.1 or SEQ ID NO.2, and a candidate gene of the molecular marker is named as AhWRI1. The KASP molecular marker is successfully developed on the basis of LTR reverse transcription transposon insertion / deletion polymorphism, the association between the KASP molecular marker and the oil content of the seed kernel is verified in a Yuhua 15 * Yueyan 20 recombinant inbred line population and a germplasm resource material, the accuracy of the marker is proved, and the KASP molecular marker can be applied to high-oil-content molecular marker-assisted selection breeding of the peanut seed kernel.

The invention discloses a brown planthopper resistant major gene Bph46 of rice and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the Bph46 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the Bph46 gene is as shown in SEQ ID NO. 2. Experiments prove that the Bph46 gene is introduced into insect-susceptible rice, so that the resistance of the insect-susceptible rice to brown planthoppers can be remarkably enhanced; on the contrary, the Bph46 gene in the insect-resistant rice is knocked out, so that the resistance of the insect-resistant rice is lost, and the function of the gene is confirmed. In addition, the invention also provides a molecular marker 12M19.042 closely linked with the Bph46 gene, a primer sequence of the molecular marker 12M19.042, and a method for detecting the Bph46 gene by using the marker. The Bph46 gene provided by the invention provides a new gene resource for brown planthopper-resistant breeding of rice, and the linked molecular marker of the Bph46 gene can be used for molecular marker-assisted selection to accelerate the breeding process of insect-resistant varieties.

The application belongs to the technical field of wheat breeding, and particularly relates to a detection marker of a main-effect gene qRS-2A of resistant starch content in wheat. The linkage marker is a SNP marker AX-110945017, the SNP marker is located on the long arm of the 2A chromosome, and the 36th base is an A / G allele, and there are AA genotypes with high resistant starch content and GG genotypes with low resistant starch content. Based on the SNP site and the corresponding KSAP marker, the resistant starch content in the wheat kernel can be accurately distinguished, and thus a good technical foundation can be laid for early screening of wheat germplasm resources with different resistant starch contents in the process of wheat molecular breeding, and subsequent selection of wheat varieties with target traits.