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Molecular marker for QTL/major gene related to verticillium wilt resistance of cotton

A technology for cotton Verticillium wilt and main effect genes, applied in the field of biotechnology applications, can solve the problem of low saturation

Active Publication Date: 2015-01-28
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies provide useful information for disease resistance breeding, but the saturation level of the existing genetic map construction is not high, and there are not many markers available for production practice, and most of the QTL positioning adopts F 2 Generation groups, mainly focus on the detection of a single generation or a single environment, and there are few stable QTLs in multiple environments

Method used

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  • Molecular marker for QTL/major gene related to verticillium wilt resistance of cotton
  • Molecular marker for QTL/major gene related to verticillium wilt resistance of cotton
  • Molecular marker for QTL/major gene related to verticillium wilt resistance of cotton

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Screening molecular markers

[0030] The present invention is linked with the molecular marker of cotton verticillium wilt resistance QTL / main effect gene, is screened out by following method:

[0031] (1) The upland cotton Zhongmian Institute 36 was used as the recurrent parent, and the Haidao Mianhai 1 was used as the donor parent to prepare a hybrid backcross combination. China Cotton Institute 36 (Zhong 36) is an early-maturing and excellent upland cotton popularization variety (National Approved Cotton 990007), and Hai 1 (Hai1) is a sea-island cotton cultivar with high resistance to Verticillium wilt (Jing Shenrong et al., 1990, China Agricultural science).

[0032] In the summer of 2003, the parents crossed and obtained the 36×Hai 1 f 1The seeds were backcrossed in the wild cotton plantation test base of China Cotton Research Institute in Hainan in the winter of 2003, and the reincarnation parent Zhong Cotton Research Institute 36 was used as the fem...

Embodiment 2

[0042] Example 2: Molecular marker selection method for improved resistance to Verticillium dahliae in upland cotton

[0043] Using the molecular marker obtained in Example 1, the marker linked to qDI-5-1 is DPL0247 110 and CIR224 160 ; marker linked to qDI-5-2 is PGML03048 270 、NAU4034 220 and BNL1042 150 ; the marker linked to qDI-5-3 is DPL0063 130 、HAU0746 210 、TMB1296 180 、CGR6708 110 and DPL0724 210 ; the marker linked to qDI-5-4 is TMB1120 390 、CGR5590 160 、PGML02063 210 、CGR5025 160 and HAU1712 220 ; The marker linked to qDI-5-5 is MGHES6 190 、DPL0838 160 、NAU2494 210 、DPL0138 240 and DPL0241 130 , carry out molecular marker selection in the breeding population related to Sea Island Cotton Sea 1 etc., comprising the following steps:

[0044] (1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent and the upland cotton variety or strain as the recipient parent, carry out hybridization and backcrossing to obtain a segregated population, or ...

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Abstract

The invention discloses an SSR marker interlocked with verticillium wilt resistance QTL / major gene loci qDI-5-1, qDI-5-2, qDI-5-3, qDI-5-4 and qDI-5-5 of cotton. The qDI-5-1, the qDI-5-2, the qDI-5-3, the qDI-5-4 and the qDI-5-5 are all located on a chromosome C5. By utilizing the SSR marker interlocked with verticillium wilt resistance major gene loci of cotton to carry out assistant selection, breeding efficiency of verticillium wilt resistance of cotton can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology applications, and relates to a SSR (Simple Sequence Repeat, simple repeat sequence) molecular marker linked to a Verticillium wilt resistance QTL / main effect gene linked with a highly resistant Verticillium wilt sea island cotton sea 1, which can be used Molecular marker-assisted selection for Verticillium wilt resistance in cotton, in order to improve the efficiency of selection and speed up the pace of breeding. Background technique [0002] Cotton is an important commercial crop, providing most of the world's natural fibers. my country is one of the largest cotton production and consumption countries in the world, and cotton production has an important strategic position in the entire national economy. It is very important to select and plant high-yielding, high-quality and disease-resistant cotton varieties. Verticillium wilt is one of the main diseases in cotton production. It is distributed in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 石玉真卢全伟袁有禄李文坦李爱国葛瑞华张保才李俊文刘爱英李骏智杨泽茂王涛龚举武商海红巩万奎陈婷婷
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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