13 results about "Verticillium dahliae" patented technology

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

Provided in the present invention are Talaromyces purpureogenus and use thereof in the prevention and control of a cotton disease. The deposit number of the Talaromyces purpureogenus is CCTCC NO: M 20242093. A further aspect of the present invention provides use of a Talaromyces purpureogenus CEF642N strain, which is used as a biological control agent for cotton Verticillium wilt. In the present invention, the inhibitory effect of a Talaromyces purpureogenus CEF642N extract component on Verticillium dahliae is analyzed, and it is found that the Talaromyces purpureogenus CEF642N extract has the effect of preventing and controlling Verticillium wilt, and can be used for preparing a biological control agent, thereby providing a new biological control means for the prevention and control of Verticillium wilt.

The application discloses a dsRNA molecule targeting VdIRE1 a gene and application thereof in prevention and treatment of verticillium wilt, and belongs to the technical field of biotechnology. VdIRE1 The application specifically discloses a dsRNA molecule which can target a Verticillium dahliae gene (coding the amino acid sequence shown in SEQ ID NO: 3) and inhibit expression of the gene, and a nucleotide sequence of a sense strand of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the application further provides a recombinant carrier containing a coding sequence of the molecule, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The schemes can be realized by means of host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment, and can effectively reduce virulence of a pathogenic bacterium, thereby providing a new technical approach for green prevention and control of verticillium wilt.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

This invention discloses a *Streptomyces xylose-lyticus* strain P6-2-1 antagonistic to multiple plant pathogenic fungi and its applications. *Streptomyces xylose-lyticus* P6-2-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36794, and is classified as *Streptomyces xylose-lyticus*. Streptomyces xylanilyticus The *Streptomyces xylose-degrading* P6-2-1 strain is effective against *Alternaria alternata*, *Fusarium solanum*, *Fusarium oxysporum* race 1 (Cuba specialized type), *Fusarium oxysporum* race 4 (Cuba specialized type), and *Fusarium graminearum*. 、 Verticillium dahliae exhibits inhibitory effects and a broad spectrum of inhibition. It can be effectively used to control plant diseases including apple rot, apple leaf spot, apple replant disease, banana wilt, cotton wilt, and corn stem rot, showing promising prospects for biocontrol applications.

The application discloses a natural sesquiterpene compound with an agricultural pathogenic bacteria resisting effect and a preparation method and application thereof. A novel natural sesquiterpene compound is separated from stems of diseased Gordonia aculeata. Activity experiments show that the natural sesquiterpene compound has the agricultural pathogenic bacteria resisting effect, has good antibacterial activity on plant pathogenic bacteria Rhizopus stolonifer, Verticillium dahliae Kleb, Thanatephorus cucumeris and Fusarium oxysporum, and can be applied to plant disease prevention and treatment as an agricultural fungicide.

This invention belongs to the field of agricultural microbiology technology, specifically relating to a microbacterium strain and its application in plant disease control. This invention discloses a microbacterium strain screened from wolfberry roots, named... Microbacterium sp. R5-1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 34797, has the 16S rDNA sequence shown in SEQ ID NO:1. It secretes siderophores, cellulase, and protease. Experimental results demonstrate that this strain exhibits inhibitory activity against the growth of *Fusarium oxysporum*, *Rhizoctonia solani*, and *Verticillium dahliae*, and shows significant control efficacy against soil-borne fungal diseases of plants caused by these fungi. This invention provides an important microbial resource for the green control of soil-borne fungal diseases of plants.

The present application belongs to the field of agricultural microorganism technology, and particularly relates to a Pseudomonas aeruginosa strain and application thereof Pseudomonas aeruginosa R5‑2, and the strain number is CGMCC No.34796.The strain can secrete iron carrier, chitinase and other active substances, and can effectively inhibit the growth of multiple pathogenic fungi such as Fusarium oxysporum, Verticillium dahliae, Rhizoctonia solani and Fusarium graminearum.The experiment shows that the fungicide containing the strain has a significant prevention and treatment effect on the root rot of wolfberry caused by Fusarium oxysporum and the tomato wilt.The present application provides a new, environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of wolfberry, tomato and other crops, and has important application value.

A Talaromyces purpureogenus and an application thereof in control of cotton diseases, a deposit number of the Talaromyces purpureogenus is CCTCCNO: M20242093. An application of the Talaromyces purpureogenus CEF642N is an application as a biocontrol agent for cotton Verticillium wilt. In the present disclosure, after analyzing the inhibitory effect of extract components of Talaromyces purpureogenus CEF642N on Verticillium dahliae, it is found that an extract of Talaromyces purpureogenus CEF642N has the effect of controlling Verticillium wilt and can be used to prepare a biocontrol agent, thereby providing a new biocontrol method for the control of Verticillium wilt.

This invention provides the application of a *Trichoderma harzianum* strain Th01 in the control of *Cotinus coggygria* wilt disease. The *Trichoderma harzianum* strain was isolated in April 2025 from the rhizosphere soil of healthy *Cotinus coggygria* plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of *Cotinus coggygria* wilt disease, shows significant inhibitory effects against *Verticillium dahliae*, promotes root repair while secreting auxin, and possesses the ability to colonize directionally in the rhizosphere soil of diseased *Cotinus coggygria* plants and continuously inhibit the germination of pathogenic microsclerotia. The *Trichoderma harzianum* strain... Trichoderma harzianum It was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42309. It can be used to control Fusarium wilt in Cotinus coggygria and can be made into a fermentation broth for the control of Fusarium wilt in Cotinus coggygria, showing good application prospects.

This invention discloses a pathogenic gene, protein, and application of *Verticillium dahliae*. This invention relates to the field of biotechnology and provides a method for controlling *Verticillium dahliae* wilt based on RNAi (RNA interference), along with its related components and applications. Specifically, it discloses the method of inhibiting or silencing the pathogenic gene of *Verticillium dahliae*. VdHDAC The application of the expression method (whose encoded protein is shown in SEQ ID NO:3) for the prevention and control of Verticillium wilt relies on providing a class of dsRNA molecules that can target this gene, with the positive strand preferably selected from SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:11. Based on this molecule, this invention further provides recombinant vectors containing its coding sequence, recombinant microorganisms, transgenic disease-resistant plants, and RNAi pesticide formulations. These methods can be achieved through host-induced gene silencing, microorganism-mediated gene silencing, or formulation treatment, effectively reducing the virulence of the pathogen and providing a new technical approach for the green control of Verticillium wilt.

This invention provides the application of Bacillus subtilis strain Bs25 in the control of Fusarium wilt in Cotinus coggygria. The Bacillus subtilis strain was isolated in April 2025 from the rhizosphere soil of healthy Cotinus coggygria plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of Fusarium wilt in Cotinus coggygria, showing a significant inhibitory effect on Verticillium dahliae. While secreting auxin, it promotes root repair and possesses the ability to colonize the rhizosphere soil of diseased Cotinus coggygria plants and continuously inhibit the germination of pathogenic microsclerotia. The Bacillus subtilis strain was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36538. Its microbial inoculum remains active under high temperature, high salinity, strong alkalinity, or extreme drought conditions. It can be directly used to control Fusarium wilt in Cotinus coggygria and has promising application prospects.