51 results about "Verticillium dahliae" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

The invention relates to the field of biotechnology application, and discloses a GhHSL1 gene and application thereof in regulation and control of upland cotton verticillium wilt resistance. The nucleotide sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 1, and the amino acid sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 2. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to preliminarily research the function of the cotton GhHSL1 gene in cotton verticillium wilt resistance, and the result shows that the verticillium wilt resistance of cotton plants is obviously reduced after the GhHSL1 gene in upland cotton is silenced; after the GhHSL1 gene is over-expressed in upland cotton, the verticillium wilt resistance of the cotton is remarkably improved, and it is determined that the GhHSL1 gene positively regulates the resistance of the upland cotton to verticillium dahliae; the invention discloses the application of the GhHSL1 gene in regulating and controlling the disease resistance of upland cotton verticillium wilt for the first time, and provides theoretical guidance and genetic resources for improving the disease resistance of cotton through molecular design breeding or genetic engineering.

The application belongs to the field of antibacterial activity research, and discloses a preparation method of guaiane sesquiterpenes and application thereof in resisting plant pathogenic fungi. Firstly, leaf blades of Artemisia stolonifera are dried, extracted, concentrated, and then a crude fraction is obtained through extraction, and then the crude fraction is further purified through normal silica gel, dextran gel and reversed-phase macroporous resin column chromatography, and finally three guaiane sesquiterpenes are obtained through liquid phase preparation. The compounds can effectively inhibit the growth of Sclerotinia sclerotiorum, Cochliobolus sativus and Verticillium dahliae, and the minimum inhibitory concentration (MIC) of moxartenolide is 6.25, 12.5 and 12.5 μg / mL respectively, the minimum inhibitory concentration (MIC) of artemdubolide C is 12.5, 12.5 and 12.5 μg / mL respectively, and the minimum inhibitory concentration (MIC) of artemvulactone F is 12.5, 12.5 and 12.5 μg / mL respectively, and the compounds have great application potential in resisting plant pathogenic fungi.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. The invention discloses Pseudomonas aeruginosa R5-2, and the number of the Pseudomonas aeruginosa R5-2 is CGMCC (China General Microbiological Culture Collection Center) No.34796. The invention further discloses a preparation method of the Pseudomonas aeruginosa R5-2. The strain can secrete various active substances such as siderophores and chitinase, and can effectively inhibit growth of various pathogenic bacteria such as fusarium oxysporum, verticillium dahliae, rhizoctonia solani and fusarium graminearum. Experiments show that a fungicide containing the strain has a remarkable prevention and treatment effect on lycium barbarum root rot and tomato fusarium wilt caused by fusarium oxysporum. The invention provides a novel and environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of crops such as medlar, tomato and the like, and has important application value.

The invention discloses a dsRNA molecule targeting a VdIRE1 gene and application of the dsRNA molecule in prevention and treatment of verticillium wilt, and belongs to the technical field of biology. The dsRNA molecule can target a verticillium dahliae VdIRE1 gene (encoding an amino acid sequence as shown in SEQ ID NO: 3) and inhibit expression of the verticillium dahliae VdIRE1 gene, and a positive-sense strand nucleotide sequence of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a chalcone emulsion in water, application of the chalcone emulsion in water in resisting cotton verticillium wilt and a prevention and treatment method. The chalcone emulsion in water comprises the following components in percentage by mass: 25% of chalcone, 30% of solvent oil 150, 7-8% of pesticide emulsifier 600 and 0.1% of xanthan gum. The preferable formula of the 25% chalcone emulsion in water is obtained through screening, and indoor and field bacteriostasis tests prove that the 25% chalcone emulsion in water can inhibit the growth of verticillium dahliae, reduce the disease index of cotton wilt and verticillium wilt, reduce the influence of cotton wilt and verticillium wilt on the agronomic traits of cotton plants in the boll period, and improve the cotton boll period. The composition is used for preventing and treating cotton verticillium wilt or cotton blight and verticillium wilt, and the prevention and treatment method is simple and effective.

The invention provides a rare earth element-based cotton verticillium wilt control composite microbial agent and a preparation method thereof, and the preparation method comprises the following steps: dissolving a rare earth compound in water, heating, stirring, adding a catechin compound, reacting, standing, cooling to obtain a mixed solution, adding a bacterial liquid into the mixed solution, and compounding to obtain the rare earth element-based cotton verticillium wilt control composite microbial agent. And storing the rare earth element-based cotton verticillium wilt prevention and control compound microbial agent at the temperature of-80 to-4 DEG C for later use. The composite microbial agent is adsorbed to cell walls of verticillium dahliae through electrostatic interaction of rare earth elements to interfere physiological metabolism of the verticillium dahliae, meanwhile, the microbial agent is released into the verticillium dahliae, and proliferation of the verticillium dahliae is inhibited through antagonism of bacillus polymyxa YUPP-8 and bacillus subtilis YUPP-2. The rare earth compound and the biocontrol bacteria are jointly applied to the field of prevention and treatment of cotton verticillium wilt for the first time, and a new technical means is provided for prevention and treatment of cotton diseases.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of preventing and treating verticillium wilt by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHAT1 (the encoding protein of the verticillium dahliae pathogenic gene VdHAT1 is as shown in SEQ ID NO: 3), the core of the verticillium dahliae pathogenic gene VdHAT1 is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

Provided in the present invention are Talaromyces purpureogenus and use thereof in the prevention and control of a cotton disease. The deposit number of the Talaromyces purpureogenus is CCTCC NO: M 20242093. A further aspect of the present invention provides use of a Talaromyces purpureogenus CEF642N strain, which is used as a biological control agent for cotton Verticillium wilt. In the present invention, the inhibitory effect of a Talaromyces purpureogenus CEF642N extract component on Verticillium dahliae is analyzed, and it is found that the Talaromyces purpureogenus CEF642N extract has the effect of preventing and controlling Verticillium wilt, and can be used for preparing a biological control agent, thereby providing a new biological control means for the prevention and control of Verticillium wilt.

The invention relates to application of turmeric carbon dots and a preparation thereof in growth promotion and bacteriostasis of cotton, and aims to overcome the defects of limited bacteriostasis effect, photosynthesis inhibition and ecological damage of existing chemical agents in prevention and treatment of cotton verticillium wilt. According to the method, a biomass-sourced turmeric carbon dot (CDs) solution (with the concentration of 25-100 mg / L, preferably 50 mg / L) is applied in a foliage spraying (seedling stage disease-resistant preparation) or soil root irrigation (blocking pathogenic bacteria infection) dual mode, mycelial growth and spore germination of verticillium dahliae are directly inhibited, meanwhile, acquired resistance (SAR) of a cotton system is activated, and root development and photosynthesis in a seedling stage are promoted. According to the method, organic unification of'prevention effect improvement, growth promotion and ecological safety 'of the nano material in prevention and control of soil-borne diseases is realized, and revolutionary technical support is provided for green agriculture.

The application discloses a dsRNA molecule targeting VdIRE1 a gene and application thereof in prevention and treatment of verticillium wilt, and belongs to the technical field of biotechnology. VdIRE1 The application specifically discloses a dsRNA molecule which can target a Verticillium dahliae gene (coding the amino acid sequence shown in SEQ ID NO: 3) and inhibit expression of the gene, and a nucleotide sequence of a sense strand of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the application further provides a recombinant carrier containing a coding sequence of the molecule, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The schemes can be realized by means of host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment, and can effectively reduce virulence of a pathogenic bacterium, thereby providing a new technical approach for green prevention and control of verticillium wilt.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The invention discloses a plant protein GhREVs, a coding gene and application of the plant protein GhREVs and the coding gene. The invention provides any one of the following applications of the GhREV protein or the coding gene thereof: A1) regulating the verticillium wilt resistance of plant cotton; a2) regulating and controlling plant disease resistance caused by verticillium dahliae; and A3) regulating and controlling the lignin content in the plants infected with verticillium dahliae. Experiments prove that silence of the GhREVs reduces the resistance of the cotton to verticillium dahliae, and the GhREVs positively regulates and controls the resistance of the cotton to V.dahliae.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Bv47 in crop growth promotion and verticillium dahliae antagonism. The strain can secrete indoleacetic acid, cellulase, protease and amylase, has various growth promoting functions of dissolving potassium, producing ammonia and the like, has good high temperature resistance and ultraviolet light resistance, and has the preservation number of CGMCC No.36499. A plate confrontation test shows that the strain has a remarkable inhibition effect on various plant pathogenic bacteria including verticillium dahliae. A pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be obviously reduced by inoculating the strain, and meanwhile, the growth indexes such as the root length, the plant height, the stem diameter, the fresh weight, the leaf area and the root dry weight of cotton plants are obviously promoted. The strain provides an efficient and green microbial resource for biological control of cotton verticillium wilt and cotton growth promotion, and has a good agricultural application prospect.

The invention belongs to the technical field of biological prevention and control of plant diseases, and particularly relates to application of streptomyces rust GJ15 in prevention and control of cotton verticillium wilt and growth promotion. The invention provides a streptomyces rubignus GJ15, and the preservation number of the streptomyces rubignus GJ15 is CGMCC (China General Microbiological Culture Collection Center) No.36046. The streptomyces rubignus GJ15 can be used for preparing a strain of streptomyces rubignus GJ15. The strain GJ15 has been preserved in the China General Microbiological Culture Collection Center on September 23, 2025, and the preservation number is CGMCC NO.36046. The China General Microbiological Culture Collection Center is called CGMCC for short, and the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The streptomyces rust GJ15 provided by the invention is free of ecological toxicity, high in safety and relatively simple to culture, has an antagonistic effect on verticillium dahliae, can obviously improve the resistance of cotton plants to verticillium wilt caused by verticillium dahliae, and can promote the growth of cotton at the same time.

The invention discloses an application method for controlling cotton verticillium wilt by utilizing terbinafine to target verticillium dahliae sterol binding protein VdPBP1, and relates to the technical field of cotton control, and the method comprises the following application steps: S1, confirming the characteristics of a terbinafine medicament; s2, formulating a terbinafine application scheme; and S3, verification and guarantee of prevention and control effects. According to the method, theoretical support is provided for precise prevention and control; the EC50 of the terbinafine is 10.8 ng / mL, the dosage of the medicament can be greatly reduced, the monovalence of the terbinafine is 30.4 yuan / g, so that the large-scale application cost is lower, the terbinafine is only combined with verticillium dahliae VdPBP1 and blocks mediated electron transfer of the verticillium dahliae VdPBP1, and the existing medicaments such as phenazino-1-carboxylic acid can inhibit histone acetylation and benomyl has wide action targets of interfering microtubulin, so that the application range of the medicament is widened. Non-target organisms are easily influenced; moreover, an ergosterol synthesis pathway is an essential pathway for fungi, and the VdERG1 is a single copy gene in verticillium dahliae, so that pathogenic bacteria are difficult to generate drug resistance through gene mutation, and prevention and control nodes widely cover the whole growth period.

The invention discloses a bacillus, which is bacillus sp. A1, and the preservation number of the bacillus is CCTCC (China Center for Type Culture Collection) M 20252592. The invention also provides a microbial inoculum product containing the strain and an application of the microbial inoculum product. The invention discloses bacillus A1 (Bacillus sp. A1) which has antagonistic activity and can effectively inhibit cotton verticillium wilt for the first time, the strain is separated from cotton rhizosphere soil, and experimental research proves that the bacillus A1 has a strong antagonistic effect on the pathogenic bacterium verticillium dahliae causing the cotton verticillium wilt, not only can significantly inhibit the growth and reproduction of the verticillium dahliae, but also can effectively inhibit the cotton verticillium wilt. The method can also practically reduce the incidence risk of cotton verticillium wilt, and shows good application potential in preparation of microbial fertilizers and functional microbial agents. In addition, the characteristics of the bacillus A1 are highly matched with the environmental conditions of the Xinjiang region, and a core strain support is provided for research and development of efficient, green and environment-friendly microbial fertilizers matched with local characteristics.

This invention discloses a *Streptomyces xylose-lyticus* strain P6-2-1 antagonistic to multiple plant pathogenic fungi and its applications. *Streptomyces xylose-lyticus* P6-2-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36794, and is classified as *Streptomyces xylose-lyticus*. Streptomyces xylanilyticus The *Streptomyces xylose-degrading* P6-2-1 strain is effective against *Alternaria alternata*, *Fusarium solanum*, *Fusarium oxysporum* race 1 (Cuba specialized type), *Fusarium oxysporum* race 4 (Cuba specialized type), and *Fusarium graminearum*. 、 Verticillium dahliae exhibits inhibitory effects and a broad spectrum of inhibition. It can be effectively used to control plant diseases including apple rot, apple leaf spot, apple replant disease, banana wilt, cotton wilt, and corn stem rot, showing promising prospects for biocontrol applications.