261 results about "Drug effect" patented technology

The invention relates to the technical field of intelligent anesthesia precise regulation and control, and discloses an anesthetic dosage optimization method based on artificial intelligence. According to the method, a dynamic treatment interval is constructed, and the boundary of the dynamic treatment interval is adaptively adjusted according to the real-time sedation depth and the nociceptive stimulation level. And in the interval, performing pattern recognition on the continuous electroencephalogram signals and the hemodynamic parameters, and marking abnormal events deviating from a standard anesthesia state. And establishing a correlation network of the drug effect chamber concentration and the abnormal events, and generating a virtual drug response curve for predicting the trend of the abnormal events under different doses. Whether dose strategy reconstruction is started or not is determined by comparing the goodness of fit between the prediction curve and the actual physiological trajectory. During reconstruction, contribution weights of historical drug infusion points to abnormal events are backtracked and analyzed, and adjustment coefficients are distributed and integrated into a new infusion sequence. According to the invention, individualization and self-adaptive optimization of anesthesia administration are realized, and the accuracy and safety of anesthesia depth control are improved.

The invention discloses a detection method for rapid real-time drug effect evaluation and application of the detection method. The detection method comprises the following steps: providing a quantitative corresponding relation between a same-generation calibration change value of electrical characteristics caused by physiological changes such as cell same-representative characteristics and the like and the cell proliferation ability; adhering to-be-detected cells to the surface of the same membrane electrode; applying the medicine to be detected, and observing the same generation measurement change value of the electrical characteristics in a cell proliferation cycle; and based on the quantitative corresponding relationship, obtaining the drug effect of the corresponding real-time proliferation characteristic evaluation drug. According to the detection method provided by the invention, the physiological changes such as the same-representative characteristics of the adherent cells are monitored through the membrane electrode, and after the quantitative corresponding relation between the change indexes of the same-representative characteristics of the cells and the proliferation capacity of the cells is established, the cell proliferation capacity is detected; the change of the cell proliferation capacity can be predicted through physiological changes such as the same representative characteristics of the cells before proliferation and apoptosis of the cells, so that the judgment period of the influence of the medicine on the cells can be greatly shortened, and the progress of medicine evaluation and research and development can be remarkably accelerated.

The invention provides a diketone biphenyl compound serving as a VAV1 degradation agent and application of the diketone biphenyl compound. Specifically provided are a compound represented by formula (I), a tautomer, a stereoisomer, a pharmaceutically acceptable salt or a prodrug thereof. The compound provided by the invention is good in drug effect and can be used for preparing drugs for treating or preventing VAV1 related diseases.

The invention discloses guanfacine hydrochloride sustained-release pellets and a preparation method thereof, and belongs to the technical field of biological medicines. The guanfacine hydrochloride sustained-release pellet comprises a drug-containing pellet core and a sustained-release layer, wherein the sustained-release layer is coated on the outer surface of the drug-containing pellet core; the medicine-containing pill core comprises the following components: guanfacine hydrochloride, a filling agent, a sustained-release material and a lubricating agent; and the sustained-release layer comprises the following components: a retardant, a plasticizer and an anti-sticking agent. The preparation method comprises the following steps: firstly, preparing guanfacine hydrochloride and auxiliary materials into a drug-containing pill core through an extrusion-rolling process, wherein the drug-containing pill core contains part of a sustained-release material for preventing sudden release of a drug; and then coating a sustained-release layer outside the drug-containing pellet core through fluidized bed coating to prepare the guanfacine hydrochloride sustained-release pellet. The guanfacine hydrochloride sustained-release pellet is free of burst release after being taken, is not influenced by individual differences of human bodies and pH of intestinal tracts, can tolerate peristalsis and extrusion of gastrointestinal tracts, is continuously released within 24 hours, keeps a proper concentration level in in-vivo blood, and is beneficial to reducing adverse reactions and continuously exerting drug effects.

The invention relates to the field of medicinal emulsifiable paste, in particular to compound fluconazole emulsifiable paste and a preparation method thereof. The compound fluconazole emulsifiable paste is prepared from the following components in parts by weight: 0.6 to 5 parts of fluconazole, 0.2 to 2 parts of neomycin sulfate, 0.1 to 0.2 part of triamcinolone acetonide, 5 to 15 parts of stearic acid, 10 to 25 parts of white vaseline, 2 to 8 parts of glyceryl monostearate, 0.5 to 3 parts of medicinal dimeticone, 1 to 5 parts of polysorbate-80, 0.1 to 0.3 part of sorbic acid, 0.1 to 1 part of vitamin E, 0.01 to 0.1 part of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 5 to 15 parts of glycerol and the balance of purified water, wherein the total amount is 100 parts. According to the application, the antioxidant property of the cream is improved by adding the vitamin E and the EDTA disodium, the vitamin E is used for directly scavenging free radicals, the EDTA disodium is used for eliminating an oxidation catalyst, and the quality guarantee period of the cream can be remarkably prolonged and the drug effect can be maintained by combining the vitamin E and the EDTA disodium.

The invention provides a traditional Chinese medicine preparation quality detection method and system based on multi-dimensional data analysis, and belongs to the technical field of traditional Chinese medicine preparation quality detection. The method comprises the following steps: acquiring mass spectrum data and nuclear magnetic resonance data of various active components in a preparation to generate a multi-dimensional chemical characteristic data set; synchronously collecting corresponding data of the medicinal raw materials, and constructing a process and environment association database; constructing a chemical and process coupling analysis model; and monitoring the production process in real time to obtain the dynamic chemical fingerprint spectrum. By constructing a full-component chemical map and associating the full-component chemical map with process and environmental parameters and combining clinical curative effect data to establish a drug effect and component response curved surface model, the limitation that only a few index components are concerned in traditional detection is broken through, so that a detection result can accurately reflect the multi-target and overall regulation characteristics of the traditional Chinese medicine preparation, and the detection accuracy is improved. The problem that the components are qualified but the curative effect is poor due to single detection dimension is avoided, and the relevance between quality detection and the clinical curative effect is remarkably improved.

The application relates to the technical field of cough-relieving medicines, and discloses a high-efficiency cough-relieving loquat particle composition which is composed of the following components in parts by weight: loquat leaf extract 30-40 parts; white front extract 10-15 parts; platycodon grandiflorum extract 6-10 parts; white mulberry bark extract 18-25 parts; stemona sessiliflora extract 7-10 parts; menthol 0.1-0.5 part; polyethylene glycol 2-5 parts; mannitol 8-12 parts; ethyl cellulose 3-6 parts; hydroxypropyl methyl cellulose 1-3 parts; phosphatidylcholine 1-3 parts; polysorbate 80 1-3 parts; cross-linked sodium carboxymethyl cellulose 4-6 parts; and sucrose 40-60 parts. The double dispersion system combining the immediate-release component and the sustained-release component is adopted, the solubility and the initial release speed of the traditional Chinese medicine extract are remarkably improved through the addition of polyethylene glycol and mannitol, and the technical effect that the drug effect appearing time is remarkably shortened is achieved. Compared with the technical scheme relying on only a single release mode in the prior art, the deficiency that the drug effect is slow and the symptoms of the patient cannot be timely relieved is solved.

The invention discloses antisense oligonucleotides for reducing XDH gene expression in a targeted manner and application of the antisense oligonucleotides, and belongs to the technical field of biological medicines. The antisense oligonucleotide mediates the degradation of the target mRNA through an RNase H (RNase H) dependent cleavage mechanism, and the mRNA expression of the XDH gene is horizontally knocked down after transcription; the antisense oligonucleotide molecule has a specific nucleic acid sequence, is combined with chemical modification design, can specifically bind XDH mRNA molecules in a targeting manner, promote XDH mRNA degradation and inhibit XDH protein synthesis, so that the expression level of XDH in cells is reduced, uric acid synthesis is reduced, the purpose of reducing uric acid is finally achieved, and cell experiments and animal model tests show that the antisense oligonucleotide molecule has a good application prospect. The antisense oligonucleotide molecule disclosed by the invention has a good uric acid reducing effect, and is long in drug effect duration and high in drug safety.

The invention provides a preparation method and application of graphene pain-relieving health-care paste, and the method comprises the following steps: mixing carrageenan with water to obtain a carrageenan solution, and carrying out ionization degradation on the carrageenan solution to obtain a carrageenan oligosaccharide solution; the preparation method comprises the following steps: stirring graphene oxide and a carrageenan oligosaccharide solution, cleaning to obtain a mixed solution, adding sodium alginate into the mixed solution to prepare a gel precursor, and adding an anhydrous calcium chloride solution and effective components into the gel precursor to obtain the pain-relieving gel, and drying the gel to obtain the health-care paste. The health-care ointment has the effects of promoting blood circulation, activating collaterals and inhibiting inflammation, and is suitable for various chronic pains such as rheumatic arthritis and lumbar muscle degeneration and acute pains such as traumatic injury and muscle strain; the composition is also suitable for auxiliary relieving of postoperative pain, and has safety and long-term effect. Due to the thermal conductivity of the graphene oxide in the health-care cream, local skin blood circulation can be accelerated, the permeation efficiency of the herbal extract is improved, and the medicine effect of the effective components is improved.

The invention discloses a traditional Chinese medicine liniment for treating rheumatoid arthritis and a preparation process thereof, the traditional Chinese medicine liniment is composed of a wind-dispelling and pain-relieving liquid medicine and auxiliary materials, the wind-dispelling and pain-relieving liquid medicine contains eight traditional Chinese medicines of thorny elaeagnus root, erythrina bark, kadsura longepedunculata root, waxberry root, ardisia crenata, rose mallow root, glabrous sarcandra herb and wikstroemia canescens root, and is prepared by ultrasonic extraction; the auxiliary materials comprise polyvinyl alcohol, a chitosan-acetic acid solution, glycerol, azone, methylparaben, vanillyl butyl ether and ethanol. The liniment is short in film forming time and good in stability, has excellent transdermal absorption performance and antioxidant activity, can remarkably relieve joint swelling of RA model mice, improve the pain threshold value, improve joint pathological injuries, achieve local precise long-acting administration, reduce side effects of the whole body and improve patient compliance, is suitable for clinical treatment of RA, and has a wide application prospect. The problems that an existing rheumatoid arthritis treatment medicine is large in side effect, and a traditional Chinese medicine preparation is short in efficacy duration and inconvenient to use are solved.

The invention relates to the technical field of medicines, and discloses an application of combination of bufalin and heme in preparation of a cancer treatment medicine. According to the invention, by means of an active metabonomics means, a key active metabolite-heme is locked through exogenous library screening and endogenous analysis, and the drug effect of bufalin is regulated by means of the active metabolite. Through verification, it is found that heme can cooperate with the synergistic drug effect in various cancer cell lines, and a new technical normal form based on an active metabolome for improving the drug effect of bufalin is provided. The pharmaceutical composition can improve the apoptosis induction ability of bufalin to different cancer cells, and provides powerful guiding significance for developing a novel treatment scheme in clinical medicine.

The invention relates to the technical field of medical care data processing, and discloses a medical care data processing method and system based on artificial intelligence, and the method comprises the steps: recording a medicine taking moment, a medicine effect starting moment and a medicine effect ending moment when a user takes medicines of each dose type; executing a work intensity evaluation strategy for each time period, monitoring the typewriting frequency of the user in each time period, and calculating the work intensity of each time period according to the time period score of the user; according to historical workdays, executing a drug effect intensity matching strategy, and calculating the corresponding work intensity of the drugs of each dose category under the optimal work completion degree; executing an intelligent medicine taking time period matching strategy, and calculating the optimal medicine taking time and dosage type based on the predicted working intensity of the next workday; and a night dose intelligent back-pushing module is executed, the dose type of the medicine taken before sleep in the next workday is calculated, the matching degree between the medicine use and the work performance is improved, and the work efficiency and the state stability are maintained to the maximum extent.

The invention provides application of an SNP (Single Nucleotide Polymorphism) marker of MIA2 in predicting the curative effect of an antithrombotic drug on thrombus, and belongs to the fields of molecular biology and cardiovascular medicine. According to the application disclosed by the invention, one or more of rs11845046 and rs10134365 of the MIA2 gene is found to be related to the prediction of the curative effect of dabigatran on thrombus for the first time. Gene polymorphism analysis and an ROC curve verify that the curative effect of an NVAF patient after the NVAF patient is treated by using the antithrombotic drug is related to the genotypes of rs11845046 and rs10134365 of the MIA2 gene in a patient sample, and when the genotype of the rs11845046 is GG or the genotype of the rs10134365 is CC, the drug effect of the subject taking the antithrombotic drug is good but the probability of bleeding events is increased.

The invention discloses a hypnotic traditional Chinese medicine pillow based on traditional Chinese medicine theory and ergonomics and a traditional Chinese medicine formula in the pillow, and particularly relates to the technical field of hypnotic traditional Chinese medicine pillows.The hypnotic traditional Chinese medicine pillow comprises a pillow inner body and detachable medicine bags, the detachable medicine bags are symmetrically arranged on the left side and the right side of the pillow inner body, and the detachable medicine bags are filled with traditional Chinese medicine mixtures; the traditional Chinese medicine mixture is prepared from the following raw materials in parts by weight: 1 part of rhizoma atractylodis, 1 part of rhizoma acori graminei, 0.5 part of radix angelicae, 1 part of wormwood, 1 part of rhizoma cyperi and 0.2 part of rhizoma kaempferiae. The components complement and cooperate in pharmacological action to jointly enhance the effects of tranquilizing and allaying excitement and improving sleep, the sleeping effect is improved in cooperation with a specific micronization treatment and low-temperature crushing process, and the traditional Chinese medicine sleeping effect is improved through deep integration of the traditional Chinese medicine sleeping theory, ergonomic design and a modern preparation process. The nerve soothing effect of traditional Chinese medicine and cervical vertebra comfortable supporting form a superimposed effect, and multi-dimensional upgrading from a single sleeping function to stable medicine effect, comfortable use and convenient maintenance is achieved.

The invention discloses a medicinal composition for strengthening body resistance and nourishing liver as well as a preparation method and application thereof. The preparation method of the composition comprises the following steps: weighing the 15 medicinal materials according to the formula amount, crushing the turtle shell, decocting for about half an hour, then adding the rest medicinal materials, decocting, filtering, and concentrating the water decoction to 1 / 3-1 / 5 of the water addition amount, thereby obtaining the traditional Chinese medicine composition. The healthy-energy-strengthening and liver-nourishing soup has the effects of inducing diuresis, nourishing the liver, relieving ascites due to cirrhosis and the like, can relieve the symptoms of patients suffering from cirrhosis due to deficiency of healthy energy, blood stasis and qi stagnation and water retention, and can be used for treating cirrhosis diseases. The prepared decoction is fast in oral administration absorption and fast in drug effect exertion. And the product has high safety and no adverse reaction.

The invention discloses a preparation method and a quality control method of a traditional Chinese medicine composition for stroke and middle meridian syndromes caused by extravasated blood blocking collaterals, and relates to the field of traditional Chinese medicines. The preparation method comprises the following steps: extracting salvia miltiorrhiza by adopting a composite enzymolysis-two-phase low-temperature extraction method, extracting erigeron breviscapus by adopting citric acid-ethanol extraction, extracting ligusticum wallichii by adopting double-template molecular imprinting-stir-frying with bran, extracting kudzu vine root by adopting a microwave method, adding auxiliary materials including hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose and beta-cyclodextrin, drying by adopting a centrifugal spray dryer, and packaging by adopting a packaging machine. The Maillard reaction is regulated and controlled. The method has the advantages of efficient extraction, component optimization, preparation stabilization, drug effect enhancement, adverse reaction rate reduction, quality controllability and the like.

The invention discloses a novel equivalence device structure closing and single-step repairing method and system in medical diagnosis, drug discovery and physiological status modeling, belongs to the field of medical diagnosis analysis, biological information processing and model verification, and ensures the reliability and traceability of the process through structural equivalence device detection and receipt track recording. The method monitors sequential equivalents (ABBA rectangles for checking consistency of operational orders) and conservation equivalents (triangles for checking information conservation and energy balance) during operation to find anomalies in diagnostic inference chains, drug action pathways, or physiological closed loops (such as predictor deviations, energy conservation imbalances, or model inconsistencies). When a non-equivalence condition is detected, the system selects a unique repair adapter from a geometric layer, a frame layer and a field layer for correction according to a preset priority, and the deviation metric mu is strictly reduced by one (delta mu = 1) during each repair, and is gradually converged to an equivalence closed state.

The present invention relates to thyroxine beta receptor selective agonist compounds, pharmaceutical compositions and uses thereof. Specifically, the invention relates to a compound as shown in a formula (I). The compound provided by the invention has excellent drug effect and / or specificity, and can be used for treating metabolism-related diseases.

The invention belongs to the technical field of plasters, and particularly relates to a plaster with long-term one-way moisture conduction performance and a preparation method of the plaster. After a backing layer of an original plaster is punched and subjected to oxygen / air plasma treatment, a large-molecular-weight polymer and a small-molecular-weight hydrophilic liquid reagent are sequentially applied to be combined, and the plaster with the long-term one-way moisture conduction performance is obtained. In-situ construction of a hydrophilic film on the inner surface of a hole of the drug matrix layer is carried out in cooperation with super-hydrophilic modification of the backing layer. The plaster patch disclosed by the invention can obtain long-term and efficient one-way moisture conduction and one-way perspiration functions on the premise of not influencing the drug effect, and the problems of discomfort and allergy of skin are avoided.

The invention discloses a method and system for predicting the effect of repairing lung injury by isoliquiritigenin based on machine learning, and relates to the field of drug effect prediction.The method comprises the steps that biomarker data and historical experiment repairing effect data are obtained; on the basis of molecular structure data and biomarker data, calculating a molecular interaction force index and a cell permeability index of the isoliquiritigenin, and analyzing through a multiple regression analyzer in combination with historical experiment repair effect data to obtain a repair effect coefficient; according to the repair effect coefficient, configuring an effect confidence interval, and performing range optimization on the molecular interaction force index and the cell permeability index to obtain an optimized biological activity index; and constructing an effect prediction model based on a neural network, inputting the optimized biological activity indexes into the effect prediction model, and outputting to obtain a lung injury repair effect prediction value. The problems that the prediction precision is insufficient and the prediction is too one-sided due to the lack of comprehensive analysis on a molecular mechanism and a biomarker are solved.

The invention provides a construction method and application of a humanized MECP2 gene mouse model, and relates to the field of gene engineering. The model animal with the humanized hMECP2 gene is successfully prepared, the humanized hMECP2 protein can be normally expressed in the body of the model, and the model can be used for MECP2 gene function research and screening and evaluation of human MECP2 targeted drugs and therapies. The animal model prepared by the invention can be used for rapidly establishing more different MECP2 mutation humanized disease mouse models, and is applied to drug screening, drug effect research, related nervous system disease and tumor treatment and the like aiming at human MECP2 target sites, so that the research and development process of new drugs is accelerated, the time and the cost are saved, and the drug development risk is reduced. And a powerful tool is provided for researching the functions of the MECP2 protein and screening drugs.

The invention relates to a composition and a medicament for inhibiting sprouting of wheat ears and application of the composition and the medicament, and belongs to the technical field of plant growth regulators. Experiments prove that when prohexadione calcium or ethephon is combined with S-abscisic acid for use, the effect of the composition is obviously superior to that of single use of S-abscisic acid, and the drug effect lasts for a longer time. It is proved for the first time that a synergistic effect exists between prohexadione calcium or ethephon and S-abscisic acid, the drug effect can be better achieved, and meanwhile the use amount of S-abscisic acid is remarkably reduced. The composition for inhibiting wheat pre-harvest germination provided by the invention effectively overcomes the defects of unstable effect, pesticide residue and the like in the prior art, can improve the effect of singly using S-abscisic acid, and achieves the purposes of controlling pre-harvest germination and not influencing wheat yield.

The invention discloses an application of glucopyranose galactooligosacharide in preparation of a medicine for treating and / or preventing allergic asthma. The invention finds that the glucopyranose galactooligosaccharide can obviously relieve airway inflammatory reaction of an allergen-induced allergic asthma model, including reducing airway inflammatory factor level, improving airway hyperreactivity and reducing lung inflammatory cell infiltration, and has no abnormal influence on blood routine and main organ functions; the composition has the advantages of small drug application dosage, low pharmaceutical cost, high safety, remarkable drug effect and the like, and has a wide application prospect.

Provided is a use of an oncolytic virus in the treatment of pain, especially in the treatment of cancer while relieving cancer pain. The drug effect time of the oncolytic virus for relieving cancer pain is obviously longer than that of the existing opioid analgesic, and the dual effects of treating cancer and easing pain are achieved at the same time.

The invention discloses a fusion protein and application thereof. The fusion protein comprises an anti-VEGFR2 (vascular endothelial growth factor receptor 2) antibody and an IL-10 monomer; wherein the IL-10 monomer is connected with the anti-VEGFR2 (vascular endothelial growth factor receptor 2) antibody. The fusion protein provided by the invention has the advantages of strong targeting property, small side effect, good drug effect and the like. The fusion protein can be used for preparing pharmaceutical compositions or drugs, and can be used for treating or preventing tumor diseases.

The invention provides a preparation method of HER2 targeted composite nanoparticles conjugated with a blood vessel disrupting agent, which comprises the following steps: grafting water-soluble azide-terminated poly (L-glutamic acid) with methoxy polyethylene glycol (NPLG-g-mPEG) to increase the number of effective load binding sites, bonding a cytotoxic drug SN38 with NPLG-g-mPEG through Steglich esterification reaction to obtain N3-PLG-SN38, and then adding the N3-PLG-SN38 into the HER2 targeted composite nanoparticles conjugated with the blood vessel disrupting agent to obtain the HER2 targeted composite nanoparticles conjugated with the blood vessel disrupting agent. According to the present invention, the Fc-PLG-SN38 is obtained by carrying out click chemical bonding on the Fc-III-4C peptide and the N3-PLG-SN38, by using the high affinity between the Fc-III-4C peptide and the Fc of the HER2 antibody, the antibody and the Fc-NPLG-SN38 are mixed in the water environment so as to obtain the HER2-PLG-SN38, and the HER2-PLG-SN38 and the blood vessel disrupting agent CA4P are conjugated so as to enhance the penetrating power and the drug effect on tumor cells;

The invention discloses an adjustable external application bellyband based on pancreatitis, and belongs to the field of medical instruments, the adjustable external application bellyband comprises a medicine application section, a bandage and a connecting assembly, and the connecting assembly is located on one side of the medicine application section and used for being connected with the bandage; the adjusting assembly is used for adjusting the tightness degree of the bandage according to the waistline size, the abdominal swelling degree and the comfort degree of the patient, and it is ensured that the bellyband can stably wrap the abdomen without generating too tight pressure feeling; then the pressure of the affected part corresponding to the medicine application section is adjusted in a targeted mode through an adjusting assembly in the medicine application section, and the situation that due to too small pressure, a medicine bag is not tightly attached, the medicine effect is lost, or due to too large pressure, pain of a patient is caused, and local blood circulation is affected is avoided; finally, an abutting assembly on an adjusting assembly mounting frame is matched with a clamping assembly, the medicine bag is firmly connected to the pancreatitis affected part in an abutting mode, it is guaranteed that the medicine bag is in tight contact with the skin all the time, and stable conditions are provided for medicine ingredient permeation and treatment effect exertion.

The present application provides the use of the volatile oil of Magnolia biondii in the preparation of a drug for chronic prostatitis. The present application also provides a pharmaceutical composition for treating chronic prostatitis, which comprises the volatile oil of Magnolia biondii and the volatile oil of Lavandula angustifolia; the weight ratio of the volatile oil of Magnolia biondii to the volatile oil of Lavandula angustifolia is 1.5-2.5:0.5-1.5. The present application also provides the preparation method and use of the extract. The present application further develops the medicinal function of the volatile oil of Magnolia biondii and makes a relevant study on the treatment of chronic prostatitis. The combination of the volatile oil of Lavandula angustifolia and the volatile oil of Magnolia biondii can better treat chronic prostatitis. The volatile oil of Lavandula angustifolia and the volatile oil of Magnolia biondii are prepared into a cream preparation, which is applied to the prostate of a rat with chronic prostatitis, and the drug effect is exerted through transdermal administration, thereby avoiding the side effects of the gastrointestinal tract and the prostate barrier, and achieving a synergistic effect of raw materials, which provides a new idea for the administration route for treating chronic prostatitis.

The invention provides a bow roundworm infection animal model as well as an establishment method and application thereof, and aims to establish a stable and repeatable bow roundworm infection animal model and explore the application value of the bow roundworm infection animal model. By optimizing the egg separation and culture technology, the development rule of infectious eggs is determined; a quantitative infection strategy is adopted to dynamically monitor worm egg discharge dynamics, clinical symptoms and blood index changes, and the reliability of the model is comprehensively evaluated; the model is further utilized to perform curative effect verification on a commercial anthelmintic drug Blein, and the result shows that the constructed animal model infected by the cat head roundworm can be used for drug effect evaluation.

The invention discloses a method and a device for detecting the energy metabolism level of cells and organoids. According to the detection method disclosed by the invention, the acidification rate and the oxygen consumption rate of the extracellular microenvironment are obtained through signal response of hydrogen ions and the dissolved oxygen sensing electrode, and meanwhile, a dynamic detection microcavity is constructed by utilizing a metabolism detection device, so that the sensitivity and the accuracy of measurement are improved. By adopting different drug intervention schemes, the energy metabolism pathway of the cells can be regulated and controlled, so that the evaluation of the two energy metabolism pathways of glycolysis and aerobic respiration of the cells is realized. The in-situ and high-sensitivity cell energy metabolism detection method and device are provided by detecting the concentration change of the specific analyte of the extracellular microenvironment, the method has the universality of two-dimensional and three-dimensional cell models, a brand new platform is provided for scientific research and drug effect screening based on an in-vitro model, and the application prospect is wide.