The application discloses an effective part of animal
medicine small-molecule
peptide and a preparation method and broad-spectrum antitumor application thereof. The preparation method comprises the following steps: taking one or more of geckos, centipedes, scorpions, earthworms, leeches, turtle shells, deer horns, silkworms, cicadas, earthworms, and nine insects, and the like, performing
water extraction, and performing
ultrafiltration through a 2.5-3.5 kD
ultrafiltration membrane, collecting an
ultrafiltration permeate with a molecular weight less than 3 kD, and concentrating to obtain the effective part. The effective part is not limited to the animal
medicine source, and also includes products with the same characteristics obtained by expression of a
genetic engineering host
cell (such as a
plant cell or a microbial
cell). The effective part has a high
impurity and pyrogen removal rate, a small-molecule
peptide enrichment rate of greater than or equal to 40%, a significant
inhibitory effect on
human lung cancer,
liver cancer, gastric
cancer,
breast cancer and various
solid tumor cells, an induction of
tumor cell apoptosis,
low toxicity to normal cells and good safety. The application has the advantages of simple process, green
environmental protection, industrial production, and the like, is suitable for various sources, and provides a new material basis and production platform for development of broad-spectrum antitumor drugs.