116 results about "Metabolic regulation" patented technology

The invention discloses a method for preparing Lactobacillus casei microcapsule bacterial powder by spray drying, and aims to provide a method which can enhance drying resistance and product viable count of Lactobacillus casei by using various activity protection techniques and can realize large-scale production. The method comprises the following steps: Lactobacillus casei viable bacteria culture obtained by high density culture adopts pre-culturing or heat activation treatment as the metabolic regulation means, and the protector is made from trehalose, sucrose, sodium borate, sodium glutamate or glutathione and vitamin C; and the mixture of skimmed milk powder and beta-cyclodextrin is used as a wall material, a Lactobacillus casei thallus suspension containing the protector is used as a core material, and spray drying is carried out to obtain the Lactobacillus casei microcapsule bacterial powder. In the spray drying process, the charging temperature is 25 DEG C, the inlet air temperature is 130 DEG C, and the pressure of an atomizer is 150 kPa. The survival rate of the bacterial powder is higher than 60%, and the viable count is more than 1010 per g.

The present invention discloses a solid drink having weight losing and body slimming functions. The solid drink is prepared from the following raw materials according to a certain proportion: soybean protein isolate, whey protein, L-carnitine, tartrate, chitin, conjugated linoleic acid glyceride micro-capsule powder, a cassia seed extract, skim milk powder, collagen powder, soybean lecithin, a lotus leaf extract, a white kidney bean extract, polydextrose, isomaltooligosaccharide, enzyme powder, konjac flour, sodium carboxymethyl cellulose and excipients of essence pigments and maltodextrin. The solid drink takes into account the comprehensive needs of people taking the solid drink in nutritional supplement, metabolic regulation, satiated meal, taste in taking, etc., avoids the common problems of the existing weight losing products to the human body, contains the necessary functional components for the weight losing, also considers the accessory functions of regulating gastrointestinal functional regulations, blood lipids and cardiovascular and cerebrovascular health, improving skin, etc., fully blends and supplements the various functional components, plays the weight losing and body slimming effect, and also has a certain health-care role.

The invention discloses a streptomyces and a multi-antibiotics metabolic regulation fermentation process. According to the method, nutrient media components are optimized, inorganic salt is added to disturb a metabolic network of streptomyces aureochromogenes, and a target production way is strengthened, so that the generation of products is promoted. Meanwhile, the multi-antibiotics fermentationprocess is optimized, and a method of flowing-feeding glucose is adopted for a feed supplement batch fermentation method, so that the fermentation unit and the production output are greatly improved,and the production cost is reduced.

The invention discloses application of Inonotus obliquus polysaccharide components in preventing and controlling chronic pancreatitis and belongs to the technical field of medicaments. The Inonotus obliquus polysaccharide components are applicable to preventing and controlling chronic pancreatitis. The Inonotus obliquus polysaccharide components which are 0.4g / kg of mouse weight are effective in increasing the content of superoxide dismutase in pancreatic glands of mice with chronic pancreatitis and decreasing accumulation of malondialdehyde and meanwhile are capable of reducing IL-1Beta and IFN-Nu in serum, and effective in decreasing the contents of amylase and lactic dehydrogenase and decreasing synthetic hydroxyproline. An application method of Inonotus obliquus polysaccharide components alleviate the condition of chronic pancreatitis in terms of immunity and metabolic regulation; compared with methods such as accumulated heat and dyspepsia conditioning in traditional Chinese medicine and medicated analgesia and surgery in western medicine, the method has the advantages that no toxic or side effect occurs, surgical pain is avoided, a treatment course is short, treatment is safe and convenient, and the effect of preventing and treating chronic pancreatitis in mice is significant.

The invention discloses an egg-laying hen feed containing Chinese herbal medicine components. The feed comprises the following materials: astragalus, polygonum multiflorum, lycium barbarum, salvia miltiorrhiza, hawthorn, and the balance of common complete feed. Compared with the common complete feed, the laying rate of egg-laying hen fed by the feed disclosed by the invention is improved by 3.2%,the prevalence is reduced by 3%, and the mortality and elimination rate is reduced by 3%. Compared with eggs laid by egg-laying hen fed by the common complete feed, the lecithin content of the eggs fed by the feed disclosed by the invention is improved by 89.3%, the content of cholesterol is reduced by 27.1%, and the nutritional value of the eggs is obviously improved. The eggs laid by the egg-laying hen fed by the feed disclosed by the invention can play the metabolic regulation role on blood fat of people, and have the health-care effects of enhancing immunization, resisting arteriosclerosis and preventing and curing cardiocerebrovascular diseases.

The invention relates to a method for adding alpha-ketoglutarate dehydrogenase inhibitor to realize excessive accumulation of alpha-ketoglutaric acid, which belongs to the technical field of the metabolic regulation optimized fermentation process of the protein level. The method of the invention comprises following steps: utilizing multi - vitamin - auxotrophic yeast of Torulopsis glabrata CCTCC M202019 as producing strains, regulating the activity of alpha-ketoglutarate dehydrogenase through adding the alpha-ketoglutarate dehydrogenase inhibitor: hydrogen peroxide, methotrexate, sodium hypochlorite or hydroxyamino in culture medium, purposively lowing the activity of the alpha-ketoglutarate dehydrogenase, reducing the degradation of the alpha-ketoglutaric acid in the metabolic process, and achieving the aim of the excessive accumulation of the alpha-ketoglutaric acid. The method of the invention cuts off carbon metabolism flow on a node of the alpha-ketoglutaric acid through regulating the stream distribution of carbon metabolism and the carbon metabolism flow, wherein the maximum output of the alpha-ketoglutaric acid reaches 23.2g / L, which is increased by 12.2% compared with the control. The invention provides a new thought for the fermentation research of TCA cycle intermediate metabolite.

The invention relates to a strain for producing L-lactic acid and a method for producing the L-lactic acid through saccharifying and fermenting Jerusalem artichoke simultaneously with the strain, which belongs to the technical fields of biological energy source and conversion technology, enzymes and metabolic regulation and control technology, as well as related fermentation engineering. The method uses aspergillus niger and lactobacillus to convert the Jerusalem artichoke into the L-lactic acid through a novel process of synchronous saccharification and fermentation. An aspergillus niger strain SL-09 with high inulinase vitality and a lactobacillus G-02 for producing the L-lactic acid with high optical purity are obtained from the natural world; when the two strains are mixed for culture, the inulinase vitality of the aspergillus niger is improved significantly due to the obvious synergy; in a lactic acid fermentation process, the Jerusalem artichoke powder is used as a substrate; and with a feed-batch fermentation process and after 36 hours of fermentation, the concentration of the L-lactic acid is 120.5 grams per liter and the conversion rate is improved to 94.5 percent. The invention provides a basis for the industrialization of the transformation of the Jerusalem artichoke to the lactic acid.

The present invention relates to (1) a polypeptide containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:7 (A chain), (2) a polypeptide containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:8 (B chain), (3) a polypeptide wherein the A and B chains are bonded to each other via disulfide bonds (2 chains) or its salt, (4) DNAs encoding the same, etc. These polypeptides and DNAs encoding the same can be used for the diagnosis, treatment, prevention, etc. for diseases, including abnormalities (e.g., diabetes mellitus, etc.) in metabolic regulation (sugar metabolism, lipid metabolism, etc.) of energy sources such as carbohydrates.

In general, this invention relates to nucleic acid and amino acid sequences involved in fat metabolism regulation and the use of these sequences as targets for the diagnosis, treatment, and prevention of obesity and obesity-related diseases. In addition, the invention relates to screening methods for identifying modulators of body fat metabolism and the development of treatments for obesity and obesity-related diseases.

The invention relates to a rat-strain-knee-osteoarthritis model building method. The rat-strain-knee-osteoarthritis model building method is combined with the primary-risk-factor joint acute or chronic biomechanics effect, the clinical strain-knee-osteoarthritis pathogenetic process is efficiently simulated, a novel animal model similar to the clinical knee-osteoarthritis pathogenesis is built, and in other words, experiment rats are only subjected to anterior cruciate ligament transection technology, but meniscuses of rats are not excised, and metabolic regulation and the stress effect of the rat knee joint micro-environment are highly kept; on the basis that a rat-knee-joint instable model is built, rats is in the forced upright bearing state at the same time, certain-strength running sport loads are fed, the joint stress effect is increased in a upslope-movement mode, a knee-joint-cartilage long-term strain is simulated, a knee osteoarthritis animal model is built, the model forming efficiency of the method and the rough time required by different-period molding of the knee osteoarthritis model are explored, and the basis is laid on further studying the mechanism and the treatment means of knee osteoarthritis.

The invention discloses slimming healthy compound fiber metabolic regulation nutrition meal replacement powder. The slimming healthy compound fiber metabolic regulation nutrition meal replacement powder consists of the following formula in proportion by weight of 8.00% of oat bran, 22.00% of polydextrose, 22.26% of resistant dextrin, 2.00% of solid malt extracts, 14.09% of separated milk protein,1.00% of wheat fibers, 1.00% of oat fibers, 0.60% of composite vitamins, 0.12% of taurine, 0.08% of zinc gluconate, 1.75% of calcium carbonate, 3.00% of linseed oil powder, 2.00% of red bean powder, 1.00% of Chinese yam flour, 2.00% of barley powder, 1.00% of gordon euryale seed powder, 2.00% of Chinese jujube powder, 1.00% of Chinese wolfberry fruit powder, 3.50% of konjaku flour, 3.00% of wholemilk powder, 0.60% of yeast chromium, 0.50% of pectin and 7.50% of oat beta-glucan. The nutrition meal replacement powder disclosed by the invention not only is rich in various dietary fibers but alsocontains rich trace elements which are beneficial to glycometabolism, and is low in glycemic index; and animal experiment and clinical research both show that the slimming healthy compound fiber metabolic regulation nutrition meal replacement powder has the effects of improving postprandial blood sugar, reducing weight and alleviating abdomen type obesity, and also has certain effect of easing constipation.

The invention provides a recombinant mortierella alpina strain for heterologous expression of an MpFADS6 gene by virtue of a delta-6 desaturase gene MpFADS6 from tiny microcystis. The invention also provides an application of the recombinant mortierella alpina strain in production of EPA. By adding 1% of peony seed oil or 50g / L of peony seed pulp juice into a culture medium, the yield of the EPA is effectively increased, which has important significance for basic theory research of metabolic regulation in synthesis of fatty acid of an oil-producing fungus namely mortierella alpine and production of PUFAs. Compared with over-expression of an omega 3 fatty acid desaturase gene in the mortierella alpine, the yield of the EPA is increased by 5 times and can reach 588.5+ / -29.6mg / L.

The present invention relates to high-throughput mammalian and medium-throughput oocyte-based electrophysiological assays for identifying human TRPML3 modulators, preferably TRPML3 enhancers. Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans. The inventive electrophysiological assays, such as the two-electrode voltage-clamp technique, facilitate the identification of compounds which specifically modulate human TRPML3. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function. Compounds that enhance or block TRPML3 channel activity should thereby modulate salty taste. In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels. This invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals (given the significance of sodium and other ions to physiological functions and conditions this phenotype is likely strongly conserved in different animals). The TRPML3 gene also modulates one or more of sodium metabolism, sodium excretion, blood pressure, fluid retention, cardiac function and urinary functions such as urine production and excretion. The inventors have identified TRPML3 as encoding a salty taste receptor in primates and humans (and likely other mammals) based on gene expression assays which have determined that TRPML3 is expressed specifically in taste bud cells and not in lingual epithelial cells, similar assays that have determined that TRPML3 is specifically expressed or enriched in the top half of taste bud cells in a subset of taste cells which do not express TRPM5 or PKD2L1, prior reports that document the expression of TRPML3 in other sensory organs such as the ear (therefore further substantiating the role of TRPML3 as a 'professional' sensory gene).

The invention discloses a primary pathway transformation method under the guidance of an FK506 strain genome-scale metabolic network model, which comprises the following steps of: adding the characteristic reaction and thallus synthesis reaction of FK506 biosynthesis according to the streptomyces tsukubaensis genome sequence, and performing manual refining on the network reaction to obtain an FK506 strain streptomyces tsukubaensis genome-scale metabolic network model; and analyzing the metabolic network model, and determining the primary pathway precursor key gene of FK506 biosynthesis through a flux equilibrium analysis and minimum metabolic regulation analysis method. According to the method disclosed by the invention, the strain production level after the transformation is improved by 80-100%. The method provides efficient guidance to the establishment, research and analysis of the primary pathway of the FK506 strain, and has great application prospects in the research process of FK506 strain transformation.

The invention relates to the field of biological analysis methods, and in particular to an analysis method of cecal metabolome in a broiler chicken. The change of metabolites and metabolic pathways ofcecal contents after the broiler chicken eats a yeast culture produced at different fermentation time is detected by jointly using a gas chromatography-mass spectrometry, thereby determining the influence of the yeast culture on the cecal metabolic regulation and the metabolites of the broiler chicken. Metabolomics data show that yeast culture is used for feeding the broiler chicken, the cecal differential metabolites mainly involve in amino acid metabolism and cholesterol metabolism, and participates in fat, vitamin digestion and absorption processes.

The invention relates to a method for converting wastes through biotechnology for controlling the diseases and insect pests of wide bighead atractylodes rhizome growing in the Tianmu Mountain, wherein after hickory hulls, pine needles and camphor leaves are grinded; the piling fermentation method is adopted, the metabolic products of microorganisms are used and the metabolic regulation of microorganisms is utilized so as to catalyze and decompose partial amino acids, generate organic amino and organic acid and neutralize partial alkaloid in the hulls; toxic substances are changed to compounds with low toxicity through enzyme reaction, the converted weakly alkaline hickory hulls are spread to the cultivated fields, thus increasing the pH value of soil and ensuring that germs and insect pests are difficult to live in the alkaline environment; and bitter and aromatic leaves can repel insect pests, and the phenolic compound, tannin and alkaloid in the hickory hulls are used to control diseases and insect pests.

The invention discloses natural medicine composition for adjusting diabetes immune and metabolism functions and a preparation method of the natural medicine composition. The natural medicine composition comprises raw materials as follows: cordyceps militaris, cyclocarya paliurus leaves and ginkgo biloba extract. A pharmacology experiment proves that the level of TG (triacylglycerol), TC (total cholesterol) and LDL-C (low-density lipoprotein cholesterol) in serum of a db / db diabetic mouse can be remarkably reduced and indicates that a product researched by our company has a remarkable function of adjustment of fat metabolism and can effectively protect the liver and the kidneys. According to compatibility of medicines, immunity declining and glucolipid metabolism disorder caused by diabetes are taken as pointcut, the functions of immunity boosting and metabolic regulation of cordyceps militaris, cyclocarya paliurus and the ginkgo leaves are used for research of health-care food, and a certain scientific theoretical foundation is provided.

The invention discloses a promoter regulating expression of genes in non-secreted glandular hair. A nucleotide sequence of the promoter is shown as SEQ ID No:1. The invention further provides a preparation method of the promoter, a carrier with the same and an expression box. The promoter can regulate expression of target genes temporally and spatially, origination and development of the non-secreted glandular hair and metabolic regulation of secondary metabolite therein can be studied deeply, and the promoter can be applied in genetic engineering breeding for producing metabolite. Consequently, the promoter is of important significance in utilizing plant glandular hair tissue expression and genetic engineering breeding for producing the metabolite.

The invention uses Kluyveromyces marxianus yeast as a platform to construct a strain of knockout pyruvate decarboxylase and glycerol-3-phosphate dehydrogenase by means of genetic engineering, metabolic engineering, molecular biology and synthetic biology. The strain's growth is restored by metabolic regulation in order to improve the yield and production rate of pyruvate, and then the strain's xylose metabolism is improved, so that it has the ability to produce pyruvate with xylose. Eventually the glucose effect of the engineering strain is removed so that the engineered strain is capable of simultaneously saccharifying lignocellulose, using glucose and xylose and fermenting at high temperature to produce pyruvate. The Kluyveromyces marxianus strain YZB058 obtained by the present invention can produce pyruvate at the same time using glucose and xylose at 42 DEG C. At 42 DEG C, YZB058 is able to produce 29.21 g / l of pyruvate with 40.97 g / l of glucose and 20.37 g / l of xylose for 36 h, the production rate is at a rate of 0.81 g / l / h, and the total yield is at 0.48 g / g.

The invention discloses a high-load metabolic regulation bioreactor and a method for adopting the same to carry out waste-water treatment. The high-load metabolic regulation bioreactor comprises a double-layer barrel-shaped separator (4), a circular isolating barrel (7) and an aeration head (6), wherein the high-load metabolic regulation bioreactor is partitioned into a reaction area and a precipitation area (1) which are connected by a sludge returning gap (8); the circular isolating barrel (7) is vertically arranged in the reaction area; the aeration head (6) is arranged at the bottom part of the circular isolating barrel (7). The method comprises the steps of: mixing of waste water, pretreatment of waste water, anaerobic methanization treatment and aerobic biological treatment. The device and method adopted for treating the waste water containing acrylic acid and the waste water containing acrylate have the advantages of fast starting speed, high treatment load, stable operation and low treatment cost.

The invention discloses a hybrid nano-formulation for promoting normalization of tumor blood vessels based on tumor extensive metabolism regulation and application thereof. A nano self-assembly technology based on electrostatic interaction and hydrogen bonding is used to obtain the hybrid nano-formulation. Heparin polysaccharide macromolecules that having VEGF inhibitory activity and derivatives and active biological macromolecules thereof, or active substance targeting vascular endothelial cells are assembled with non-viral gene carriers and metabolic regulation related gene drugs to construct stable nano-drugs targeting tumor cells and tumor vascular endothelial cells, separately. After the hybrid nano-formulation formed only by physically mixing the two ternary assembled nano-drugs areadministered in the manner of beat, significant metabolic regulation effects of tumor cells and tumor vascular endothelial cells, promoting normalization of tumor blood vessels, and the multiple therapeutic effects of tumor proliferation inhibition can be obtained. The three effects are synergistic and complement each other to enhance the effectiveness.

The invention belongs to the technical field of medical technology, relates to a preparation method for increasing CK (Compound K) yield of ginsenoside, and in particular relates to a preparation method for increasing the CK yield of ginsenoside by increasing the yield of a converted product through adjustment and control in a microbial conversion process. According to the preparation method, the yield of the converted product and the concentration of a primer are improved through metabolic regulation. The preparation method comprises the steps of: reconstructing ultraviolet mutagenesis of a parental strain, culturing in a high-concentration pseudo-ginseng stem and leave saponin culture medium, sieving in a directional manner to obtain a strain resisting to 1-5% of the total saponins of the pseudo-ginseng stems and leaves. Through the strain mutagenesis and the metabolic regulation, the concentration of the primer and the yield of the converted product are improved obviously, the final conversion rate is up to 43.63%, and the yield of the Compound K of the ginsenoside is improved by more than 80%. The preparation method for increasing CK yield of ginsenoside has practical significance for the industrial application of the microbial conversion.

The invention relates to a method for preparing and regenerating aspergillus oryzae protoplast for high-yield lactase. The method comprises the following steps: (1) preparing aspergillus oryzae spore suspension; (2) performing physiochemical mutation so as to obtain a mutant strain; (3) performing enzymolysis to prepare protoplast; (4) purifying the protoplast; (5) regenerating the protoplast. The quantity of the protoplast prepared by the method disclosed by the invention is high, the obtained protoplast has high activity, and the regeneration rate can reach 31.2%. The protoplast is high in preparation rate and excellent in activity maintenance, is favorable for preparation of subsequent fusants and has significances for researching the metabolic regulation theory of aspergillus oryzae regulated lactase and improving the lactase activity; meanwhile, a novel pathway is provided for strain breeding during lactase production.

The invention discloses a meal replacement powder with the function of regulating body metabolism, and a preparation method thereof. In the meal replacement powder, grains with high fiber content, various dietary fibers, and active ingredients used as medicine and food are added, and the traditional Chinese medicine theory and modern pharmacological research results are used for scientific formulation. The functional formula powder is made into a functional food, and the efficacy of the functional formula powder is verified through animal experiments. The results show that the functional formula powder can inhibit weight gain, reduces fat accumulation in the body, improves the body's insulin sensitivity, lowers the cholesterol and triglyceride content, and has good metabolic regulation effects. The meal replacement powder is suitable for different people. The food does not contain any flavor, food coloring and preservatives, and thereby the product is safe to eat.

The invention provides an integrated fertilizing method for cultivating selenium-rich organic crops. The integrated fertilizing method comprises the following steps of on the basis of crops per mu, when the crops need to be fertilized, applying 500 to 3,000kg of carbon organic fertilizer with carbon, wherein the carbon organic fertilizer is wormcast organic fertilizer, fulvic acid organic fertilizer or a mixture of the wormcast organic fertilizer and the fulvic acid organic fertilizer; besides, applying 10 to 75kg of compound liquid probiotic fertilizer or 50 to 300kg of compound solid probiotic fertilizer, 50 to 200kg of potash fertilizer, 25 to 100kg of selenium-rich nutritional vitality element, 5 to 10kg of carbon dioxide long-acting granular fertilizer, 2 to 5kg of root irrigation cultivating agent, 50 to 150g of plant metabolic regulation hormone and 50 to 250g of plant immune regulation hormone. The integrated fertilizing method provided by the embodiment of the invention can realize the effects that grown crops are good in growth vigor and high in yield; the crops are rich in trace elements of selenium and other nutritious elements, so that the crops are more nutritional and are absorbed by human bodies more easily after being eaten.

The invention belongs to the field of biological fermentation and in particular provides an enzyme bamboo charcoal capsule product capable of whitening and fading spots and a preparation method thereof. Fermentation is performed by using a special process, the fermented enzyme stock solution is subjected to low-temperature spray granulation, and the biological activity of the probiotics is remained. On one hand, due to metabolic regulation of activated probiotics, the intestinal flora is balanced, and normal physiological functions are restored; and on the other hand, facial pigmentation is reduced by combining with super-strong adsorption of the bamboo charcoal. In addition, the capsule product has the characteristics of small size, light weight and convenience in carrying.

The invention brings forward a method for raising sirolimus fermentation yield by adding amino acid into a fermentation medium. During fermentation, the initial pH value of the medium is controlled within the range from 5.5 to 7.0, the disinfection temperature ranges from 115 DEG C to 121 DEG C and the disinfection process lasts for 30 min; simultaneously, amino acid at mass concentration of 0.2-1% is added into the initial fermentation medium, wherein the amino acid is one or more combinations selected from the group consisting of proline, phenylalanine, glutamic acid and glutamine, valine, alanine, and leucine or threonine. According to the invention, with the combination of sirolimus biosynthesis characteristics, amino acid which is related with the supply of sirolimus synthesis precursor is obtained by screening, and the addition of a certain concentration of amino acid into the initial medium can greatly raise the sirolimus fermentation level. The method provided by the invention has advantages of clear purpose and low fermentation cost and the like, can be used to conduct the metabolic regulation of the sirolimus fermentation by adding amino acid and applied for industrial production.

The invention discloses a method for preparing enterococcus faecalis microcapsule bacterium powder through a spray drying method, and aims at providing a method through which the anti-dry capacity of enterococcus faecalis can be improved and the product viable bacterium count of the enterococcus faecalis can be increased by combining multiple activity protection techniques, and large-scale production can be achieved. Preculture or heat shock treatment is adopted as a metabolic regulation means for viable enterococcus faecalis cultures obtained through high-density culturing, and a protective agent is prepared from trehalose, sucrose and vitamin c; a mixture of soybean protein and guar gum (or Arabic gum) is selected as a wall material, and enterococcus faecalis thallus suspension liquid containing the protective agent is taken as a core material; the enterococcus faecalis microcapsule bacterium powder is obtained through spray drying, wherein in the spray drying process, the inlet air temperature ranges from 80 DEG C to 120 DEG C, the outlet air temperature ranges from 60 DEG C to 80 DEG C, and the atomizer pressure is 150 kPa. The survival rate of the obtained bacterium powder is 70% or above, and the viable bacterium count is 1,011 / g or above.

The invention relates, in part, to methods and compositions that are useful to modulate metabolic function of cells in vivo or in vitro. In some aspects the invention includes methods and / or compositions that increase metabolism in cells, tissues, organs, and / or subjects. In certain aspects the invention includes methods and / or compositions useful to decrease metabolism in cells, tissues, organs, and / or in subjects.