250 results about "Metabolic regulation" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a centella asiatica glycosyl transferase gene in catalyzing glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the protein coded by the gene can catalyze the C-28 site of asiatic acid and madecassic acid to perform glucose group modification so as to generate asiatic acid monoglucoside and madecassic acid monoglucoside, and preferentially catalyzes madecassic acid. By constructing a metabolic regulation network, the genes are widely and accurately screened, and the glycosylation process of catalyzing asiatic acid and madecassic acid is analyzed; an important reference basis is provided for improving the glycosylation efficiency of asiatic acid and madecassic acid and the yield of asiaticoside and madecassoside in the in-vitro synthesis and industrial production process of the centella asiatica triterpenoid saponin, and an important theoretical support is provided for large-scale production of the centella asiatica active saponin.

The embodiment of the invention discloses a sleep peptide and differential metabolite detection method for caenorhabditis elegans, belongs to the technical field of organism metabolism, and aims to research sleep-awakening key metabolite of caenorhabditis elegans and a regulation mechanism of the sleep-awakening key metabolite. According to the sleep peptide for caenorhabditis elegans, the sleep peptide is an F4 monomer as shown in SEQ ID NO: 1. Compared with the prior art, the sleep and awakening process of the caenorhabditis elegans under the intervention of the goat milk-derived sleep peptide is analyzed through a metabonomics technology, the key metabolite for regulating and controlling sleep and awakening is successfully screened out, and a core metabolic pathway related to the key metabolite is disclosed. The application also constructs a metabolite-pathway-target regulation framework, fills the blank of sleep research in the aspect of metabolic regulation, provides a scientific basis for a sleep intervention strategy based on metabolic regulation, and also lays a foundation for developing metabolic markers of sleep states in the future.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention discloses a rice large-scale metabolism regulator DGP1 and crop metabolism quality design. The invention finds that the structural domain of the rice DGP1 family protein TIGR01589 targets a plurality of metabolic enzymes and promotes the metabolic enzymes to be transferred to a cell nucleus; the structural domain also has an intervention effect on a light breathing pathway by changing the composition of a glycine decarboxylase compound in mitochondria. Based on a molecular mechanism of DGP1, a chimeric protein ST containing a conservative structural domain and coupled with a chloroplast positioning signal is designed, and genetic engineering modification of pakchoi is realized. The modification strategy not only can guide substrate flow of a central glycolysis pathway to flavonoid biosynthesis, but also can promote accumulation of saccharides derived from photosynthetic products in leaves.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the technical field of microorganisms, in particular to saccharomyces cerevisiae CEC NW94 and application thereof. The invention provides the Saccharomyces cerevisiae CEC NW94, and the Saccharomyces cerevisiae CEC NW94 is used for brewing the wine, so that the contents of glycerol and lactic acid can be increased, and the yield of volatile acid can be reduced; the synthesis of volatile substances such as ethyl 2-methylbutyrate, isoamyl alcohol and linalool is promoted, and the generation of acetic acid is inhibited, so that the wine presents complex aroma of black fruits, mushrooms and the like, and the odor of herbaceous plants, herbal medicines, vinegar and the like is effectively reduced; in addition, the strain can improve the umami of the grape wine, and improve the aroma purity, the aroma concentration, the wine body, the aftertaste and the complexity. According to the method, the flavor substances of the dry red wine are controlled through a microbial metabolism regulation and control technology, and a new technical scheme is provided for high-quality wine production.

The invention discloses a method for screening a Wilson disease serum exosome miRNA biomarker based on a transcriptomics technology, and belongs to the field of bioinformatics analysis of diseases. According to the invention, the effect of miRNA in WD pathogenesis is discussed by identifying serum exosome miRNA, and a potential biomarker is provided for accurate diagnosis and treatment of the disease. According to the invention, 59 DE-miRNAs are identified by analyzing the expression of differential miRNAs of WD and control group patients, and the DE-miRNAs are related to key approaches such as metabolic regulation, cancer progression, signal transduction and the like. Besides, the reliability of the key DE-miRNA, including miR-451a, miR-204-5p and the like, is confirmed through experimental verification, and the potential of the key DE-miRNA as a non-invasive biomarker is indicated. Research results provide important theoretical basis and new insight for accurate diagnosis of WD and development of potential therapeutic targets.

The invention provides a dietary fiber anti-obesity and internal fat-reducing composition and a preparation method and application thereof, and the dietary fiber anti-obesity and internal fat-reducing composition comprises the following components in percentage by mass: a metabolic regulation formula which comprises 0.8-1.2% of mulberry leaf extract, 0.3-0.5% of potato extract, 0.2-0.4% of gamma-aminobutyric acid microcapsules and 4.0-6.0% of isomaltooligosacharide; the water management formula comprises 0.5 to 1.0 percent of opuntia ficus-indica powder and 0.2 to 0.4 percent of fructus gardeniae powder; the auxiliary regulation formula comprises the following components in percentage by weight: 2.0 to 4.0 percent of fructo-oligosaccharide, 0.05 to 0.15 percent of sucralose, 0.10 to 0.30 percent of xanthan gum and 0.05 to 0.15 percent of sodium citrate; and the nutrition fortification formula comprises 0.1-0.3% of taurine and 0.5-1.0% of a compound nutrition fortifier, and the compound nutrition fortifier contains vitamin B6 / B12, magnesium, iron and zinc. By constructing a fiber barrier-enzymolysis network-targeted metabolism three-in-one system, accurate reduction and control of visceral fat are achieved, fat oxidation combustion can be promoted, fat decomposition can be accelerated, fat accumulation can be reduced, and the method can be used for preparation of liquid beverages, preparation of powdery electuary and preparation of far infrared bagged powder solid beverages and has good application prospects. And the application scenarios are greatly increased.

The invention discloses a culture medium composition for culturing GDT cells and a culture method, and belongs to the field of biological medicine. Aiming at the problems of low in-vitro amplification efficiency and poor function of the existing GDT cells, the invention provides the culture medium composition for culturing the GDT cells, the culture medium composition comprises an induction culture medium and a proliferation culture medium, the induction culture medium comprises a basic culture medium and an activating agent, and the activating agent comprises IL-7 and paclitaxel; the proliferation culture medium comprises a basic culture medium, IL-18 and a metabolism regulator. An STAT5 pathway is activated through IL-7, and amplification of the Vdelta2 subgroup is specifically promoted; on the other hand, through the cooperation of the IL-7 and the IL-18, the IL-18 activates the expression of the NKG2D, and the IL-18 and the IL-7 jointly enhance the cytotoxicity; efficient amplification and function enhancement of the GDT cells are achieved; the killing rate of PD-L1 + tumor cells is larger than 80%, the motility rate after cryopreservation recovery is larger than 90%, the function maintenance rate is larger than 85%, and the cell line is suitable for tumor immunotherapy, infectious disease treatment and cell drug development.

The invention relates to technical development and application of new special medical food on the basis of a new food raw material framework, and belongs to the technical field of special medical purpose formula food (FSMP). According to the series of foods, new food raw materials (euglena, haematococcus pluvialis and ampelopsis grossedentata leaves) are taken as cores, medicinal and edible components (lotus leaves, haws and mulberry leaves) and marine active substances (fucoidan polysaccharides and chitin) are combined, functional factors are extracted through a prehydrolysis SBE technology and a biological enzymolysis technology, and the bioavailability is improved by adopting a nano-carrier technology. The product comprises a total nutrient base material (20-30% of protein and 15-25% of dietary fiber), functional components (10-15% of a new food raw material extract and 8-12% of a traditional Chinese medicine extract) and a metabolism regulator (conjugated linoleic acid and L-carnitine). Clinical tests show that after an obese patient takes 400 kcal of the replacement meal every day, the weight of the obese patient is reduced by 10.2 kg on average (4.8 kg of a control group) 12 weeks later, and the body fat rate is reduced by 16.3%. The product supports line iteration (such as powder, tablets and liquid food) through modular formula design, and fills the application blank of new food raw materials in special medical food.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a rice cake capable of regulating postprandial blood sugar and inhibiting starch aging, and belongs to the field of food processing and preservation. The rice cake is prepared from the following raw materials in parts by mass: 100 to 300 parts of polished round-grained rice flour, 100 to 200 parts of glutinous rice flour, 5 to 30 parts of pre-gelatinized corn starch, 5 to 30 parts of wheat type B starch granules and 100 to 300 parts of water. The pre-gelatinized corn starch-wheat type B starch composite preservative prepared by mixing the pre-gelatinized starch and the wheat type B starch in proportion can effectively inhibit hardness increase caused by starch aging and retrogradation in the storage process of the rice cake and avoid taste deterioration. Meanwhile, by adding the composite starch, the digestibility rate of the rice cake is remarkably reduced, and the metabolic regulation characteristic similar to that of low glycemic index food is endowed to the product. The characteristic of a cleaning label formed by natural components of the rice highly conforms to the development trend of healthy food, and the rice is especially suitable for functional rice product development of people needing to control postprandial blood sugar fluctuation.

The present application relates to the technical field of biological medicine, and in particular to a cat triple vaccine immune adjuvant containing two kinds of cat interleukins and metabolic regulatory molecules, a composition and application. The composition comprises cat interleukin 15, cat interleukin 23 and a metabolic regulatory molecule, the metabolic regulatory molecule comprises beta-nicotinamide mononucleotide, beta-nicotinamide adenine dinucleotide disodium salt, N-acetyl cysteine and L-carnitine, and can be used for preparing a cat triple vaccine or an auxiliary preparation of the cat triple vaccine. The composition can effectively improve the protection of the vaccine, prolong the protection time of the vaccine and reduce adverse reactions; the vaccine can more durably and at a higher level reduce or control the reproductive infection of viruses, significantly improve the specific humoral immune level and memory response ability of animals, and better resist the invasion and spread of FPV, FCV and FHV-1.

The invention relates to an intestinal flora derivative indolepropionic acid capable of relieving obesity and application thereof, and discloses new application of indolepropionic acid in obesity treatment. Indolepropionic acid is prepared through a microbial fermentation method, the purity is higher than that of indolepropionic acid prepared through a chemical synthesis method, multi-target metabolism regulation is achieved by activating a PPAR-gamma signal channel, and the effects of fat metabolism remodeling, liver injury repairing, glycometabolism regulation, inflammation inhibition and the like are achieved; compared with the prior art, the probiotic strain dependence is broken through, a single and definite active component, namely indolepropionic acid, is provided, and the complexity and effect fluctuation of flora intervention are eliminated; the multi-target metabolism regulation is realized, the increase of white fat browning and the reduction of liver fat decomposition are synchronously promoted, and the problem that the fat type cannot be converted by the existing metabolite is solved; the industrial feasibility is optimized, the high-purity indolepropionic acid is prepared through microbial fermentation, the cost is reduced compared with that of a chemical synthesis method, and the purity and production cost bottlenecks are broken through.

This invention discloses a acne-repairing and repairing composition specifically developed for adolescents and its application, relating to the field of cosmetic technology. The invention provides an acne-repairing and repairing composition comprising 5-12 parts of guaiac extract, 1-6 parts of eggplant extract, 0.001-0.01 parts of aescin, 0.01-0.1 parts of diosgenin, and 0.01-0.1 parts of asiaticoside. This invention achieves a three-dimensional synergistic effect of "anti-inflammatory, repair, and metabolic regulation" through the synergistic interaction of five components: guaiac extract, eggplant extract, aescin, diosgenin, and asiaticoside, forming a comprehensive management system for acne and post-acne repair in adolescents.

The invention discloses a novel active peptide composition for improving concentration and a preparation method and application thereof. Belongs to the technical field of new active peptide composition preparation. The novel active peptide composition is prepared through synergism of the novel active peptide PAVP, the blood-brain barrier penetrating peptide and the metabolism regulator, when the novel active peptide PAVP, the blood-brain barrier penetrating peptide and the metabolism regulator act together, the novel active peptide composition has a synergistic effect, and the concentration improving effect of the novel active peptide composition is better than that of each independent component; the time for the mouse to explore new things can be prolonged to a certain extent, the relative expression quantity of synaptic plasticity markers PSD-95, BDNF and p-CREB can be increased to a certain extent, and technical support is provided for research and development of concentration-related products.

The invention discloses application of metformin to improvement of crop seed germination and stress resistance, the metformin is used as a widely used hypoglycemic drug, has a cross-species conservative metabolism regulation function, can activate an antioxidant defense system of plants, maintain K < + > / Na < + > balance in the plants, and can improve the survivability of crops in saline-alkali soil. After the metformin is adopted to treat the seeds, the germination rate and the germination potential of the seeds are obviously improved, the chlorophyll content, the peroxidase content, the soluble protein content and the like of seedlings are obviously increased, the malondialdehyde content is obviously reduced, and the stress resistance is obviously enhanced. The technology is low in treatment cost, simple to operate and obvious in effect.

The invention relates to the technical field of medical treatment, and particularly discloses a training method for delaying development of Alzheimer's disease, which comprises the following steps: (a) a neural pathway activation stage: generating a dynamic three-dimensional space navigation task through a virtual reality system and displaying a color coding path to a patient; the method comprises the following steps: (c) a lymphatic driving stage: starting a 40Hz multi-mode stimulation combination after metabolic regulation reaches the standard, and (d) a closed-loop optimization stage: detecting saliva exosome A beta 42 concentration or EEG gamma wave power every two weeks, each time is 40 minutes every day, and the steps (a)-(b)-(c) are executed in sequence; neuropathology is improved, compared with the prior art, the hippocampus atrophy rate is remarkably reduced, the A beta 42 pathological deposition removal efficiency is high, and the white matter fiber integrity is improved; the cognitive function of the patient is enhanced, the space navigation capability is optimized, and the attention and work memory are improved.

The application discloses application of tomato saponin in preparation of a uric acid reducing composition and belongs to the technical field of biological medicine. The uric acid reducing composition comprises 80-240 parts of tomato saponin, 50-120 parts of momordica charantia saponin, 25-100 parts of fruit and vegetable extract, 10-20 parts of schizonepeta turczanovii extract, 10-20 parts of terminalia catappa leaf extract and 40-100 parts of soluble dietary mixture. Animal experiment verification shows that the composition can effectively reduce blood uric acid level and improve kidney function through the synergistic effect of multi-target points such as inhibition of uric acid synthesis, promotion of uric acid excretion, anti-inflammatory kidney protection and metabolic regulation, and a safe and efficient solution is provided for hyperuricemia.

This invention relates to a pyridoxal phosphate-responsive biosensor and its application, belonging to the field of synthetic biology biosensor technology. This invention is the first to discover and identify the relationship between pyridoxal phosphate and Cgl1185-P. cgl2838 The transcriptional regulation and response relationship between these factors was investigated, and this relationship was used as the basis for constructing a biosensor responsive to pyridoxal. The transcription factor regulatory system Cgl1185-P derived from Corynebacterium glutamicum was used. cgl2838 A biosensor for the specific detection of pyridoxal phosphate was constructed by assembling a reporter gene as the main component onto a plasmid, enabling effective detection of pyridoxal phosphate concentrations in intracellular or culture environments. This biosensor possesses advantages such as clear structure, high sensitivity, and good host adaptability, and can be used for fermentation process monitoring, cofactor metabolism research, and screening of relevant strains, showing promising application prospects in the fields of cofactor concentration monitoring and metabolic regulation.

The invention relates to a colon-targeted micro-ecological regulation composition. The colon-targeted micro-ecological regulation composition is prepared by compounding the following functional components in percentage by weight: 5-95% of a component A, 1-15% of a component B and 4-94% of a component C, the component A is resistant starch, the polymerization degree DP is greater than or equal to 15, and the crystallinity is greater than or equal to 35%; the component B is probiotics and is embedded through a complex coacervation method or an extrusion-gel method, the embedding rate is larger than or equal to 75%, and the particle size is 200-800 microns; the survival rate is greater than or equal to 60% after treatment in simulated gastric juice for 2 hours, and the survival rate is greater than or equal to 50% after treatment in simulated intestinal juice for 4 hours; the component C comprises functional nutritional auxiliary materials, the functional nutritional auxiliary materials comprise prebiotics and a medicinal and edible composition, and the prebiotics comprise rapid fermentation type prebiotics, medium-speed fermentation type prebiotics, slow fermentation type prebiotics and a non-fermentation type water-holding expansion component. By constructing a carbon source slow release-flora orientation-metabolism regulation ternary interaction collaborative network, accurate regulation and multi-target metabolism intervention of the whole digestive tract are realized.

The invention discloses a substance basis and a detection method for the drug effect of liver-bean jelly, and belongs to the technical field of medicines. In-vitro chemical components of liver-bean jelly are combined to track blood entering components of liver-bean jelly and carry out attribution analysis; obtaining an action target spot of the liver-bean linging for intervening the WD hepatic fibrosis; screening metabolic markers for significant callback of liver-bean linging, and performing enrichment analysis to obtain a significant metabolic pathway; constructing a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network by the action target and the significant callback metabolic marker, and screening a core action target, a core metabolic marker and a core metabolic pathway; a molecular docking technology is used for screening a liver-bean drug effect material basis, and a multi-dimensional network of the liver-bean drug effect material basis, a core action target, a core metabolic marker, a core metabolic pathway and WD liver fibrosis is visualized. The invention discloses a pharmacodynamic material basis and a metabolic regulation mechanism for treating WD hepatic fibrosis by using liver bean paste.

The invention relates to small yellow ginger compound protein powder as well as a preparation method and application thereof, belongs to the technical field of food, and provides small yellow ginger compound protein powder which takes small yellow ginger extract microcapsules and soybean protein isolate powder as main components. According to the invention, the small yellow ginger extract and the soybean protein isolate are combined for use, and the metabolic regulation function of the small yellow ginger is combined with the nutritional supplement function of the protein powder, so that the functionality of the protein powder is improved; good metabolism promoting, muscle building and weight losing effects can also be achieved.

The present invention relates to the technical field of pharmaceutical preparations, and specifically to a selenized carrageenan vitamin complex, a preparation method and application thereof. The vitamin complex comprises the following components in parts by mass: 33-37 parts of selenized carrageenan, 8-12 parts of modified vitamin E, 10-14 parts of modified vitamin C, 0.5-1 parts of modified folic acid, 1-2 parts of vitamin B6, 4-8 parts of magnesium glycinate, and other raw materials. The present invention achieves efficient compounding and functional synergy of multiple active ingredients through an innovatively designed biological carrier network and molecular modification technology. Compared with the existing technology, the stability, bioavailability and targeted delivery ability of nutrients are significantly improved, and breakthrough advantages are demonstrated in metabolic regulation and anti-oxidation. The technology is suitable for health industries such as functional food development and has broad market prospects.

This invention belongs to the field of biopharmaceutical technology and specifically relates to the use of NT5M in the preparation of a drug for the treatment of pancreatic cancer. The invention reveals that NT5M is a key metabolic regulator in PAAD, influencing tumor progression, immune regulation, and drug sensitivity. Low NT5M expression is associated with poor prognosis, while its overexpression inhibits cell migration, invasion, and PD-L1-mediated immune evasion, highlighting its potential as a tumor suppressor in PAAD. It can serve as both a prognostic biomarker and a potential therapeutic target, providing a new treatment strategy for PAAD patients.

The application provides a method for improving the adenosine content of Ophiocordyceps sinensis based on metabolic regulation and synergistic induction, wherein a metabolic intervention is performed on the larva and strain complex using a regulating agent during the infection period before low-temperature treatment to form the infected larva; first, deep low-temperature immobilization treatment is performed at 2-5 DEG C for 30-50 days, then culture is performed using a variable-temperature cycle with diurnal temperature difference during the stroma induction period, and mannitol is sprayed onto the surface of the culture medium at the beginning of the step; after harvesting, rapid temperature rising enzyme inactivation and drying treatment is performed on the harvested Ophiocordyceps sinensis, the rapid temperature rising enzyme inactivation and drying treatment comprises increasing the drying temperature from 40-45 DEG C to 60-70 DEG C within 30-45 minutes and maintaining the temperature until the moisture content is less than 10%. The application can solve the problem that the adenosine content is generally low in the existing full artificial cultivation technology, and can make the adenosine content of the full artificial cultivation Ophiocordyceps sinensis reach more than 0.020% (w / v), which is significantly better than the existing artificial cultivation level, and the product morphology is complete and the reproducibility is high.

The invention provides a composition for promoting ATP (adenosine triphosphate) generation and improving kidney energy metabolism by activating an AMPK / PGC-1alpha pathway. The composition is prepared from the following raw materials in parts by weight: 100 to 400 parts of herba cistanche, 50 to 200 parts of radix astragali seu hedysari, 25 to 100 parts of glycine betaine and 150 to 300 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for improving ATP generation and improving kidney energy metabolism by activating the AMPK / PGC-1alpha pathway. Through combined use of cistanche, astragalus membranaceus, betaine and alpha-ketoglutaric acid, a multi-target synergistic mechanism of AMPK activation, metabolic substrate supplementation and epigenetic regulation is formed, the mitochondrial function and ATP generation of kidney cells are comprehensively improved, the limitation of existing single-component or chemical drugs is broken through, a safer and more efficient intervention strategy is provided for kidney energy metabolism disorders, and the clinical application prospect is broad. The invention provides an innovative solution for renal tubular energy disorder, has dual values of metabolic regulation and kidney protection, and conforms to the development trend of natural medicines. The invention belongs to the technical field of food and health care product processing.

The invention relates to the technical field of grease processing, and discloses a method for increasing the sn-2 DHA content in schizochytrium limacinum triglyceride. The method comprises the following steps: adding a metabolic regulator into a fermentation culture solution containing schizochytrium limacinum, carrying out fermentation culture until the total fermentation time is more than 120 hours, and collecting a grease product, the metabolic regulator is selected from at least one of choline chloride, inositol and lysophosphatidic acid. According to the method provided by the invention, the lipid metabolic flux of schizochytrium limacinum can be effectively regulated and controlled, and directional enrichment of DHA to the sn-2 site of triglyceride is remarkably promoted, so that the yield and content of sn-2 DHA are improved.

The embodiment of the invention provides an oxadiazole-steroid compound, a pharmaceutical composition and application. The oxadiazole-steroid compound is a compound with a structure as shown in a formula I or pharmaceutically acceptable salt of the compound or a mixture of the compound and the pharmaceutically acceptable salt. The oxadiazole-steroid compound provided by the embodiment of the invention can effectively activate a metabolic regulation target FXR, and has excellent potential for preventing or treating MAFLD and related complications thereof. Meanwhile, the compound has no excitation effect on an MRGPRX4 receptor possibly causing pruritus while maintaining the FXR excitation activity, and a safer choice is provided for treatment of related diseases. Therefore, the oxadiazole-steroid compound has a more remarkable application prospect in preparation of the medicine for treating the metabolism-related fatty liver disease.

The invention discloses application of auricularia auricula monomer polysaccharide ME-2 in preparation of drugs for preventing and treating fatty liver diseases related to metabolic dysfunction. The research of the invention systematically proves that ME-2 has a remarkable intervention effect on the whole course of MASLD for the first time. The invention discloses a breakthrough function of ME-2 as a liver metabolism regulator for the first time: by directly correcting liver lipid metabolism disorder (such as regulating core genes such as Srebp-1c, ACC1, FASN and the like), liver fatty degeneration can be relieved in a dose-dependent manner in the whole course of MASLD, and the serum transaminase level and dyslipidemia can be reduced. Importantly, the curative effect of the traditional Chinese medicine is derived from intervention on a metabolic root source, and is totally different from a known anti-inflammatory application mechanism. Meanwhile, ME-2 has the remarkable advantages of being clear in structure, taking metabolic regulation as a core, and being safe and non-toxic, and a brand new drug candidate is provided for prevention and treatment of MASLD.