116 results about "Metabolic regulation" patented technology

The invention discloses a method for preparing Lactobacillus casei microcapsule bacterial powder by spray drying, and aims to provide a method which can enhance drying resistance and product viable count of Lactobacillus casei by using various activity protection techniques and can realize large-scale production. The method comprises the following steps: Lactobacillus casei viable bacteria culture obtained by high density culture adopts pre-culturing or heat activation treatment as the metabolic regulation means, and the protector is made from trehalose, sucrose, sodium borate, sodium glutamate or glutathione and vitamin C; and the mixture of skimmed milk powder and beta-cyclodextrin is used as a wall material, a Lactobacillus casei thallus suspension containing the protector is used as a core material, and spray drying is carried out to obtain the Lactobacillus casei microcapsule bacterial powder. In the spray drying process, the charging temperature is 25 DEG C, the inlet air temperature is 130 DEG C, and the pressure of an atomizer is 150 kPa. The survival rate of the bacterial powder is higher than 60%, and the viable count is more than 1010 per g.

The invention discloses application of Inonotus obliquus polysaccharide components in preventing and controlling chronic pancreatitis and belongs to the technical field of medicaments. The Inonotus obliquus polysaccharide components are applicable to preventing and controlling chronic pancreatitis. The Inonotus obliquus polysaccharide components which are 0.4g / kg of mouse weight are effective in increasing the content of superoxide dismutase in pancreatic glands of mice with chronic pancreatitis and decreasing accumulation of malondialdehyde and meanwhile are capable of reducing IL-1Beta and IFN-Nu in serum, and effective in decreasing the contents of amylase and lactic dehydrogenase and decreasing synthetic hydroxyproline. An application method of Inonotus obliquus polysaccharide components alleviate the condition of chronic pancreatitis in terms of immunity and metabolic regulation; compared with methods such as accumulated heat and dyspepsia conditioning in traditional Chinese medicine and medicated analgesia and surgery in western medicine, the method has the advantages that no toxic or side effect occurs, surgical pain is avoided, a treatment course is short, treatment is safe and convenient, and the effect of preventing and treating chronic pancreatitis in mice is significant.

The invention relates to a method for adding alpha-ketoglutarate dehydrogenase inhibitor to realize excessive accumulation of alpha-ketoglutaric acid, which belongs to the technical field of the metabolic regulation optimized fermentation process of the protein level. The method of the invention comprises following steps: utilizing multi - vitamin - auxotrophic yeast of Torulopsis glabrata CCTCC M202019 as producing strains, regulating the activity of alpha-ketoglutarate dehydrogenase through adding the alpha-ketoglutarate dehydrogenase inhibitor: hydrogen peroxide, methotrexate, sodium hypochlorite or hydroxyamino in culture medium, purposively lowing the activity of the alpha-ketoglutarate dehydrogenase, reducing the degradation of the alpha-ketoglutaric acid in the metabolic process, and achieving the aim of the excessive accumulation of the alpha-ketoglutaric acid. The method of the invention cuts off carbon metabolism flow on a node of the alpha-ketoglutaric acid through regulating the stream distribution of carbon metabolism and the carbon metabolism flow, wherein the maximum output of the alpha-ketoglutaric acid reaches 23.2g / L, which is increased by 12.2% compared with the control. The invention provides a new thought for the fermentation research of TCA cycle intermediate metabolite.

The invention relates to a rat-strain-knee-osteoarthritis model building method. The rat-strain-knee-osteoarthritis model building method is combined with the primary-risk-factor joint acute or chronic biomechanics effect, the clinical strain-knee-osteoarthritis pathogenetic process is efficiently simulated, a novel animal model similar to the clinical knee-osteoarthritis pathogenesis is built, and in other words, experiment rats are only subjected to anterior cruciate ligament transection technology, but meniscuses of rats are not excised, and metabolic regulation and the stress effect of the rat knee joint micro-environment are highly kept; on the basis that a rat-knee-joint instable model is built, rats is in the forced upright bearing state at the same time, certain-strength running sport loads are fed, the joint stress effect is increased in a upslope-movement mode, a knee-joint-cartilage long-term strain is simulated, a knee osteoarthritis animal model is built, the model forming efficiency of the method and the rough time required by different-period molding of the knee osteoarthritis model are explored, and the basis is laid on further studying the mechanism and the treatment means of knee osteoarthritis.

The present invention relates to high-throughput mammalian and medium-throughput oocyte-based electrophysiological assays for identifying human TRPML3 modulators, preferably TRPML3 enhancers. Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans. The inventive electrophysiological assays, such as the two-electrode voltage-clamp technique, facilitate the identification of compounds which specifically modulate human TRPML3. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function. Compounds that enhance or block TRPML3 channel activity should thereby modulate salty taste. In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels. This invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals (given the significance of sodium and other ions to physiological functions and conditions this phenotype is likely strongly conserved in different animals). The TRPML3 gene also modulates one or more of sodium metabolism, sodium excretion, blood pressure, fluid retention, cardiac function and urinary functions such as urine production and excretion. The inventors have identified TRPML3 as encoding a salty taste receptor in primates and humans (and likely other mammals) based on gene expression assays which have determined that TRPML3 is expressed specifically in taste bud cells and not in lingual epithelial cells, similar assays that have determined that TRPML3 is specifically expressed or enriched in the top half of taste bud cells in a subset of taste cells which do not express TRPM5 or PKD2L1, prior reports that document the expression of TRPML3 in other sensory organs such as the ear (therefore further substantiating the role of TRPML3 as a 'professional' sensory gene).

The present invention relates to (1) a polypeptide containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:7 (A chain), (2) a polypeptide containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:8 (B chain), (3) a polypeptide wherein the A and B chains are bonded to each other via disulfide bonds (2 chains) or its salt, (4) DNAs encoding the same, etc.These polypeptides and DNAs encoding the same can be used for the diagnosis, treatment, prevention, etc. for diseases, including abnormalities (e.g., diabetes mellitus, etc.) in metabolic regulation (sugar metabolism, lipid metabolism, etc.) of energy sources such as carbohydrates.

The invention discloses a hybrid nano-formulation for promoting normalization of tumor blood vessels based on tumor extensive metabolism regulation and application thereof. A nano self-assembly technology based on electrostatic interaction and hydrogen bonding is used to obtain the hybrid nano-formulation. Heparin polysaccharide macromolecules that having VEGF inhibitory activity and derivatives and active biological macromolecules thereof, or active substance targeting vascular endothelial cells are assembled with non-viral gene carriers and metabolic regulation related gene drugs to construct stable nano-drugs targeting tumor cells and tumor vascular endothelial cells, separately. After the hybrid nano-formulation formed only by physically mixing the two ternary assembled nano-drugs areadministered in the manner of beat, significant metabolic regulation effects of tumor cells and tumor vascular endothelial cells, promoting normalization of tumor blood vessels, and the multiple therapeutic effects of tumor proliferation inhibition can be obtained. The three effects are synergistic and complement each other to enhance the effectiveness.

The invention discloses a meal replacement powder with the function of regulating body metabolism, and a preparation method thereof. In the meal replacement powder, grains with high fiber content, various dietary fibers, and active ingredients used as medicine and food are added, and the traditional Chinese medicine theory and modern pharmacological research results are used for scientific formulation. The functional formula powder is made into a functional food, and the efficacy of the functional formula powder is verified through animal experiments. The results show that the functional formula powder can inhibit weight gain, reduces fat accumulation in the body, improves the body's insulin sensitivity, lowers the cholesterol and triglyceride content, and has good metabolic regulation effects. The meal replacement powder is suitable for different people. The food does not contain any flavor, food coloring and preservatives, and thereby the product is safe to eat.

The invention discloses a method for preparing enterococcus faecalis microcapsule bacterium powder through a spray drying method, and aims at providing a method through which the anti-dry capacity of enterococcus faecalis can be improved and the product viable bacterium count of the enterococcus faecalis can be increased by combining multiple activity protection techniques, and large-scale production can be achieved. Preculture or heat shock treatment is adopted as a metabolic regulation means for viable enterococcus faecalis cultures obtained through high-density culturing, and a protective agent is prepared from trehalose, sucrose and vitamin c; a mixture of soybean protein and guar gum (or Arabic gum) is selected as a wall material, and enterococcus faecalis thallus suspension liquid containing the protective agent is taken as a core material; the enterococcus faecalis microcapsule bacterium powder is obtained through spray drying, wherein in the spray drying process, the inlet air temperature ranges from 80 DEG C to 120 DEG C, the outlet air temperature ranges from 60 DEG C to 80 DEG C, and the atomizer pressure is 150 kPa. The survival rate of the obtained bacterium powder is 70% or above, and the viable bacterium count is 1,011 / g or above.

The invention relates, in part, to methods and compositions that are useful to modulate metabolic function of cells in vivo or in vitro. In some aspects the invention includes methods and / or compositions that increase metabolism in cells, tissues, organs, and / or subjects. In certain aspects the invention includes methods and / or compositions useful to decrease metabolism in cells, tissues, organs, and / or in subjects.