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Method for preparing enterococcus faecalis microcapsule bacterium powder through spray drying method

A spray-drying and Enterococcus faecalis technology, applied in the field of preparing Enterococcus faecalis microcapsule powder, can solve the problems of cell membrane cytoplasm and nucleic acid damage, bacterial protein coagulation and denaturation, leakage of cell contents, etc. Dehydration damage, increase the number of viable bacteria, long storage period

Inactive Publication Date: 2016-11-23
JIANGXI KENUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the destruction of the hydrogen bonds of important biopolymers such as proteins, nucleic acids, and enzyme systems, the bacterial proteins will be coagulated and denatured, and the nucleic acids will be degraded, denatured and inactivated, thereby destroying the composition of the cells; thermally dissolving the lipid-like components on the cell membrane to form a small Pores that allow leakage of cell contents and also cause damage to cell membranes, cell walls, cytoplasm, and nucleic acids, resulting in death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) High-density culture: Inoculate 10 ml of the activated Enterococcus faecalis culture into 10 liters of MRS liquid medium by aseptic operation, and culture it at 37°C for 24 hours, and the number of viable bacteria can reach 1.04×10 9 / ml.

[0020] (2) Cool the live Enterococcus faecalis culture obtained from the high-density culture in step (1) in a water bath at 4°C for 20 minutes, centrifuge to collect the Enterococcus faecalis bacterial cell precipitate, and obtain the bacterial cell precipitate of Lactobacillus dryum after centrifugation Item weight is 68g

[0021] (3) Prepare protective agent: add 4.08g trehalose, 2.72g sucrose and 0.0272g vitamin C, and mix well. Sterilize at 121°C for 15 minutes to prepare a protective agent additive solution.

[0022] (4) Add the protective agent additive liquid obtained in step (3) into the centrifuged thalline sediment of Enterococcus faecalis collected in step (2) through aseptic operation, shake for 5 minutes to make t...

Embodiment 2

[0026] (1) High-density culture: inoculate 10 ml of the activated Enterococcus faecalis culture into 10 liters of MRS liquid medium by aseptic procedure, and culture it at 37°C for 24 hours, and the number of viable cultured bacteria reaches 1.24×10 9 / ml.

[0027] (2) Cool the live Enterococcus faecalis culture obtained from the high-density culture in step (1) in a water bath at 4°C for 20 minutes, centrifuge to collect the Enterococcus faecalis bacterial cell precipitate, and obtain the bacterial cell precipitate of Enterococcus faecalis after centrifugation The weight is 71g.

[0028] (3) Preparation of protective agent: add 0.568g of trehalose, 2.84g of sucrose and 0.0284g of vitamin C, and mix well. Sterilize at 121°C for 15 minutes to make a protective agent additive solution for later use.

[0029] (4) Add the protective agent additive solution obtained in step (3) to the centrifuged bacterial sediment of Enterococcus faecalis collected in step (2) through aseptic op...

Embodiment 3

[0033] (1) High-density culture: Inoculate 10 ml of the activated Enterococcus faecalis culture into 10 liters of MRS liquid medium by aseptic method, and culture it at 37°C for 24 hours, and the number of viable bacteria in the culture reaches 1.44×10 9 / ml.

[0034] (2) Cool the live Enterococcus faecalis culture obtained from the high-density culture in step (1) in a water bath at 4°C for 20 minutes, centrifuge to collect the Enterococcus faecalis bacterial cell precipitate, and obtain the bacterial cell precipitate of Enterococcus faecalis after centrifugation The weight is 75g.

[0035] (3) Preparation of protective agent: add 0.9 g of trehalose, 1.5 g of sucrose and 0.015 g of vitamin C, and mix well. Sterilize at 121°C for 15 minutes to make a protective agent additive solution for later use.

[0036] (4) Add the protective agent additive solution obtained in step (3) to the centrifuged bacterial sediment of Enterococcus faecalis collected in step (2) through aseptic ...

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Abstract

The invention discloses a method for preparing enterococcus faecalis microcapsule bacterium powder through a spray drying method, and aims at providing a method through which the anti-dry capacity of enterococcus faecalis can be improved and the product viable bacterium count of the enterococcus faecalis can be increased by combining multiple activity protection techniques, and large-scale production can be achieved. Preculture or heat shock treatment is adopted as a metabolic regulation means for viable enterococcus faecalis cultures obtained through high-density culturing, and a protective agent is prepared from trehalose, sucrose and vitamin c; a mixture of soybean protein and guar gum (or Arabic gum) is selected as a wall material, and enterococcus faecalis thallus suspension liquid containing the protective agent is taken as a core material; the enterococcus faecalis microcapsule bacterium powder is obtained through spray drying, wherein in the spray drying process, the inlet air temperature ranges from 80 DEG C to 120 DEG C, the outlet air temperature ranges from 60 DEG C to 80 DEG C, and the atomizer pressure is 150 kPa. The survival rate of the obtained bacterium powder is 70% or above, and the viable bacterium count is 1,011 / g or above.

Description

technical field [0001] The invention relates to a method for preparing Enterococcus faecalis microcapsule powder by spray drying. Background technique [0002] Spray drying technology has the advantages of simple equipment, low cost, easy promotion, and is conducive to large-scale continuous production. It is widely used in the commercial field, especially in the pharmaceutical and food industries. Spray drying technology is beneficial to the large-scale production, transportation and storage of probiotics. However, in the spray drying process, microorganisms have to experience high temperature and dry pressure, and cell damage is mainly due to high temperature and dry dehydration. Temperature has the greatest impact on the survival of microorganisms, especially high temperature. High temperature can destroy the enzyme system of microorganisms to maintain life activities, reducing or losing their activity. Due to the destruction of the hydrogen bonds of important biopolyme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C12R1/01
Inventor 王劲松张魁
Owner JIANGXI KENUO BIOTECH
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