Compound probiotic medicinally and edibly homologous plant fermented drink and preparation method thereof

A technology of medicinal and edible homologous plants and compound probiotics, applied in the directions of bacteria, food science, Lactobacillus, etc. used in food preparation, can solve the problems of short shelf life and unstable living bacteria, achieve easy absorption and utilization, and improve health care. Efficacy, good fermentation performance

Inactive Publication Date: 2018-05-18
SHANGHAI JIAODA ONLLY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the shelf life of lactic acid bacteria beverages at low temperature is short, and the problem of unstable live bacteria is still a technical problem that those skilled in the art have been working hard to solve.

Method used

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  • Compound probiotic medicinally and edibly homologous plant fermented drink and preparation method thereof
  • Compound probiotic medicinally and edibly homologous plant fermented drink and preparation method thereof
  • Compound probiotic medicinally and edibly homologous plant fermented drink and preparation method thereof

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preparation example Construction

[0039] The present invention has no special limitation on the preparation method of the probiotic powder, which can be prepared by conventional preparation methods in the art. In the present invention, the preferred probiotic powder is prepared by the following method:

[0040] 1) Cultivate and ferment the above three kinds of probiotics in MRS culture solution or improved MRS culture solution respectively to obtain a fermentation solution containing probiotics;

[0041] 2) Centrifugation: centrifuge the three kinds of probiotic fermentation broths obtained above to obtain bacteria sludge;

[0042] 3) Freeze-drying: the bacteria slime is mixed with water and a freeze-drying carrier, and the obtained mixed material is freeze-dried to make freeze-dried live bacteria powder.

[0043] Among the present invention, the formula of described MRS culture fluid is: containing peptone 10.0g in every liter of culture fluid, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citr...

Embodiment 1

[0073] Physiological and biochemical characteristics of Lactobacillus plantarum LP-Onlly

[0074] The Lactobacillus plantarum LP-Onlly in the present invention was isolated from a healthy infant in 1989, the original strain code is 8901, and the accession number of its 16S rRNA gene partial sequence in GeneBank is AY590777. The bacterium was preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 6, 2004, and the preservation registration numbers are respectively CGMCCNO.1258. Bacteria are rod-shaped, lack flagella, but can move. Gram-positive, non-spore forming, facultatively anaerobic. A unique strain determined by the analysis of acid resistance and bile salt resistance. Its high aminopeptidase activity, high β-galactosidase activity and high glucosidase activity make its fermentation performance and genetic stability better, and it has been passed down for 15 generations. Afterwards, the genomic DNA fingerprints and HPLC fingerprints ...

Embodiment 2

[0076] Physiological and biochemical characteristics of Lactobacillus acidophilus LA11-Onlly

[0077] The Lactobacillus acidophilus LA11-Onlly in the present invention is directly isolated from the feces of healthy infants, and was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 13, 2007, with the preservation number CGMCCNo.2106. It has unique acid resistance, bile salt resistance and good antioxidant properties. Its shape is rod-shaped, Gram-positive, does not form spores, produces lactic acid, and is facultatively anaerobic. It can use galactose, glucose, fructose, mannose, mannitol, cellobiose, maltose, lactose, melibiose, sucrose, trehalose, raffinose, gentiobiose, etc. for fermentation.

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Abstract

The present invention belongs to the field of fermented drink preparation and particularly relates to compound probiotic medicinally and edibly homologous plant fermented drink and a preparation method thereof. The fermented drink comprises the following fermentation raw materials in parts by weight: 0.001-0.1 part of compound probiotic, 30-60 parts of a medicinally and edibly homologous plant water extract and 0.5-10 parts of breeding factors; wherein the compound probiotics are mixed from the following 3 bacteria in any proportions: lactobacillus acidophilus with a preservation number of CGMCC No. 2106, lactobacillus plantarum with a preservation number of CGMCC No. 1258 and lactobacillus casei with a preservation number of CNCM I-4458. A total number of the viable probiotics in the fermented drink is more than 10<9> CFU / g. Besides, after being preserved in a cold-storage condition of 0 DEG C-4 DEG C for 4 months, a number of the viable bacteria is still 10<7> CFU / ml, and shelf lifeof the product is greatly prolonged.

Description

technical field [0001] The invention belongs to the field of fermented beverage preparation, and in particular relates to a compound probiotic medicinal and edible homologous plant fermented beverage and a preparation method thereof. Background technique [0002] In recent years, combining food science and preventive medicine to carry out research, so that products have special health functions, is the direction of food science and technology development. With the continuous acceleration of the pace of life, the deterioration of the living environment and the decline of physical functions, more and more people are in a sub-health state such as fatigue, vitality, responsiveness, and adaptability. As a result, the normal balance of the human body is broken, and the body's self-regulation function is out of balance. At the same time, with the intensification of people's unhealthy lifestyles, long-term staying up late, smoking, drinking a lot of alcohol, high work pressure and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L33/00
CPCA23L2/382A23L33/00A23V2400/125A23V2400/113A23V2400/169
Inventor 贾素中杭晓敏张和春张旻
Owner SHANGHAI JIAODA ONLLY CO LTD
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