60 results about "Gentiobiose" patented technology

The invention discloses a feed additive for a laying hen. The feed additive comprises the following components by weight percent: 65-75wt% of flaxseed cake, 9-12wt% of lucid ganoderma, 8-10wt% of vinolevure, 3-6wt% of streptococcus faecium and 5-7wt% of viametto, wherein the flaxseed cake is subjected to detoxification treatment; the total mass fraction of linamarin, lotaustraline, beta-gentiobiose neolinustatin and beta-neolinustatin in the detoxified flaxseed cake is smaller than 20mg / kg; and the additive is evenly mixed into an edible feed for the laying hen by 0.4-0.5wt%. According to the feed additive, the immunity of the laying hen can be improved, the genital system function of the laying hen is enhanced, the laying rate and the egg weight are increased, the nutritive value of the feed can be improved, the yolk colors of egg products are increased, the eggshell quality is increased, the health of chicken flocks is ensured, application of antibiotics, hormone and the like in the laying hen feed is effectively reduced, the safety of egg products is improved, and the feeding benefits are improved.

The invention discloses a fructus gardeniae standard substance and a fructus gardeniae analog extracted from traditional Chinese medicine fructus and the preparation methods thereof. The fructus gardeniae analog consists essentially of fructus gardeniae, iso-fructus-gardeniae, dehydroxy fructus gardeniae (geniposide), geniposidic gentiobiose glycoside, wild fructus gardeniae, gardeniae ketoside; scandoside formate, deacetylasperuloside, geniposidic acid, 10-O-acetyl geniposidic glocoside, 6-para-cormaroyl geniposidic gentiobioside glycoside etc. The fructus gardeniae standard substance and the fructus gardeniae analog can be prepared by any of the methods such as solvent extraction, macroporous absorption resin, supercritical fluid extraction, column chromatography, fluid-fluid countercurrent chromatography, or any combination of the methods. The purity of the prepared fructus gardeniae standard substance is 98-100 percent (w / w), and the total percentage contents of iridoid components in the fructus gardeniae analog are 5-100 percent (w / w), wherein, the content of fructus gardeniae account for 5-100 percent (w / w) of the total iridoid contents.

The invention relates to an oligosaccharide water-retaining agent used for water retention of red shrimps, which is composed of following raw materials, by weight, 3-5% of gentiobiose, 25-30% of trehalose, 3-9% of a degradation product of konjac polysaccharide, 4.5-6.5% of alginate oligosaccharides, 2-3% of lactic acid, 5-8% of an anti-oxidizing agent and the balanced being dextrin. The water-retaining agent can maintain fresh degree of frozen shrimps. Volatile amino nitrogen and total bacteria are limited within a national standard range. The water-retaining agent can greatly reduce drying loss during freezing and greatly reduce drying loss of the red shrimps. Meanwhile, the water-retaining agent is prepared from natural fresh-keeping and water-retaining raw materials, thereby ensuring food safety and increasing economic benefit in aquatic product processing enterprises.

The invention relates to a method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi and belongs to the technical field of separation and purification of a compound monomer. According to the invention, the folium sauropi medicinal material is taken as a raw material, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers are acquired according to the steps of ethanol extraction, concentration, macro-porous resin enrichment and elution, polyamide resin enrichment and elution, efficient preparation, liquid chromatography separation and product recycling. According to the invention, through mature processing steps and parameter conditions, the purities of the quercetin-3-O-gentian diglucoside and the kaempferol-3-O-gentian diglucoside in the product respectively reach above 99%; and meanwhile, the method is simple and convenient in operation and is high in separating efficiency, the raw materials are recyclable, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers can be simultaneously separated and prepared in large batch and high purity.

The invention relates to a composition, and also relates to a Crocus sativus pigment active site extracted from Fructus Gardeniae. The composition mainly comprises trans-crocetin di(beta-D-gentiobiosyl)ester, trans-crocetin-beta-D-gentiobiosyl-beta-D-glucosyl ester, trans-crocetin di(beta-D-glucosyl)ester, 13-cis-crocetin di(beta-D-gentiobiosyl)ester, neocrocin B, trans-crocetin mono(beta-D-gentiobiosyl)ester, 13-cis-crocetin-8-O-beta-D-gentiobiosyl ester, 13-cis-crocetin-8'-O-beta-D-gentiobiosyl ester and trans-crocetin mono(beta-D-glucosyl)ester. Pharmacological experiment results show that the Crocus sativus pigment active site can effectively improve mouse learning memory injuries induced by scopolamine and amyloid beta protein.

The present invention provides functional rare sugar syrup and a preparation method thereof. A mass percentage of sucrose syrup in the rare sugar syrup is 0-99%. The rare sugar syrup is one or more of trehalose syrup, fructooligosaccharide syrup, allulose syrup, arabinose syrup, meso-erythritol syrup, neohesperidin dihydrochalcone syrup, stevioside syrup, maltooligosaccharide syrup, isomaltooligosaccharide syrup, galacto-oligosaccharide syrup, soybean oligosaccharide syrup, mixed cottonseed sugar, lactose and sucrose syrup, xylo-oligosaccharide syrup, mannan-oligosaccharide syrup, cyclodextrin glucose syrup, gentiobiose syrup, coupling sugar syrup, panose syrup, milk oligosaccharide syrup, chitin oligosaccharide acetylglucosamine syrup and ganoderma lucidum polysaccharide syrup. The functional rare sugar syrup has functions of protecting cells, regulating flora balance in body, regulating balances of blood sugar, blood pressure and blood lipids, protecting internal organs, promoting absorption of nutrients, preventing obesity, dental caries, oxidation and tumors, delaying aging, etc.

The invention provides a synthetic method of gentiobiose. The synthetic method of the gentiobiose comprises the following steps: taking D-glucose as a starting material; respectively synthesizing into beta-1,2,3,4-tetra-acetylated glucose and glucose-B-glucosinolate; and carrying out coupled reaction and then carrying out deprotection to generate the gentiobiose. The controllability of the technological process is high, isomerization of the product is avoided, the yield of single-step reaction is high, and the gentiobiose is suitable for being produced on a large scale. The separating efficiency of a final product is improved. By the chemical method, large-scale production of the gentiobiose is implemented, a biological-method complicated purification process is overcome, the production period is shortened, the cost is reduced, and the purity of the product is improved.

A composition suitable for inhibiting the outgrowth of fungi is provided comprising a first ingredient which inhibits the biogenesis of a normal fungal cell wall and a second ingredient which is capable of perturbing the structure of the cellular membrane of said fungi, so that either the cellular integrity is essentially lost or cell division cannot take place, or both. The first ingredient preferably inhibits the anchorage of cell wall proteins in the cell wall of the fungi and is suitably a beta-(1,6)-glucose polysaccharide, preferably beta-gentiobiose or a pustulan fragment. The second ingredient is suitably a natural microbial membrane affecting substance (MMAS), preferably MB-21. The composition is particularly suitable as a preservative for inhibiting the outgrowth of fungi in food products, such as sauces, dressings, ketchups, and soups, but is also useful for preventing or inhibiting undesired fungal growth on other products such as personal health care products, e.g. soap bars.

The invention provides a cleaning fluid for water treatment ultrafiltration membranes. The cleaning fluid for the water treatment ultrafiltration membranes is composed of deionized water, inulase, gentiobiase, citrulline, beta-amylase, zytase and aspartic protease. The deionized water in each cubic meter comprises the components of 20-60g inulase, 40-90g gentiobiase, 5-20g citrulline, 80-200g beta-amylase, 60-200g zytase and 5-10g aspartic protease. The invention further provides a method for cleaning the ultrafiltration membranes. The cleaning fluid for the water treatment ultrafiltration membranes is simple in formula and simple and easy to produce. The cleaning fluid is basically composed of active enzymes, and can effectively removing the organic pollutants in the ultrafiltration membranes.

The present invention provides a dried influenza vaccine preparation in which the activity of an influenza vaccine antigen can be stably maintained even when stored without strictly maintaining a low temperature, and which can be stably supplied. The present invention also provides a method of producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation containing an influenza vaccine antigen and a disaccharide, wherein the disaccharide is at least one selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The invention relates to a method for preparing gentiooligosaccharide by using beta-1,6-glucanase and an application of the method and belongs to the technical field of genetic engineering and fermentation engineering. The invention firstly provides beta-1,6-glucanase TcBgn with transglycosylation activity on gentiobiose, the beta-1,6-glucanase TcBgn can transform glucose into gentiooligosaccharide under the condition of relatively small additive amount of enzyme, and the transformation rate is high, so that the production cost can be significantly reduced. The gentiooligosaccharide is prepared by double-enzyme compounding of the beta-1,6-glucanase and beta-glucosidase, and the glucose is transformed into the gentiobiose and gentianose by using a double-enzyme compounded system, so that the transformation rate is high, and the proportion of the gentianose in a product is significantly improved. Therefore, the method for preparing the gentiooligosaccharide by a single enzyme of the beta-1,6-glucanase or double-enzyme compounding has good industrial application value.

The invention discloses a method for producing cellulase by mixed fermentation of aspergillus oryzae and fusarium chlamydosporum. The method comprises the following steps: inoculating the fusarium chlamydosporum and the aspergillus oryzae on a bagasse solid culture medium and performing mixed culture fermentation, wherein the culture temperature is 20-50 DEG C, and pH value is 4.0-10.0; finishing fermentation culture after the maximum cellulose yield is reached. The method disclosed by the invention has the benefits that through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, high-transglycoside activity belta-glucosidase produced by the aspergillus oryzae can synthesize gentiobiose, and the gentiobiose can stimulate the synthesis of the cellulase from the fusarium chlamydosporum; through the mixed culture of the aspergillus oryzae and the fusarium chlamydosporum, the composition of a cellulase system is optimized, so that the overall enzyme activity of the cellulase system can be significantly improved.

The present invention relates to a novel beta-1,6-endoglucanase producing gentiobiose or glucose from beta-glucan, and more specifically, the present invention provides an effect of producing gentiobiose or glucose at high yield through beta-1,6-endoglucanase showing beta-1,6-endoglucanase activity on beta-glucan.

The invention provides an extraction method of whole components of cassia seeds and in particular relates to the technical field of traditional Chinese medicine extraction. The extraction method can extract all effective components in cassia seeds and convert the effective components into finished products to the full extent by combination use of two sets of extraction methods including a supercritical CO2 extraction technology and a high-pressure differential low-temperature continuous extraction technology. Added value of products is increased, and the application amount of organic solvent is reduced while the high extraction rate of all effective components is guaranteed, and pollution is reduced. The extraction rate of total flavonoids, polysaccharides, chrysophanol, aurantio-obtusin,cassia glycoside C and cassia glycoside B2 is up to 65% or above, and especially the extraction rate of components which are high content but sensitive to temperature in raw materials as protein and erythromycin and gentiobiose glycoside is up to 65% or above, and extraction steps are simplified so that production cost is saved. The extraction method is simple to operate and easy to repeat and issuitable for realizing standard industrial production.

The invention discloses a functional beverage capable of neutralizing the effect of alcoholic drinks and invigorating the stomach. The functional beverage is prepared by mixing an extract A, an extract B, an extract C, an additive and water according to the weight ratio of the extract A to the extract B, to the extract C, to the additive to the water being 5 to 3 to 1.5 to 3 to 100, wherein the extract A is prepared from the following substances of kudzuvine roots, raisin tree seeds, flavescent sophora roots, semen lablab album, licorice roots, and stringy stonecrop herb; the extract B is prepared from the following substances of calyx kaki, flos mume, coix seeds, haws, wrinkled gianthyssop herb and colocasia esculenta stems; the extract C is prepared from the following substances of pinellia tubers, rhizome cyperi, and inula flowers; and the additive comprises the following substances of vitamin B1, vitamin B2, vitamin B9, vitamin E, zinc gluconate, taurine, aspartame, gentiobiose, and aminofusin. The functional beverage disclosed by the invention has good efficacies of neutralizing the effect of alcoholic drinks and invigorating the stomach, can relieve symptoms of gastrointestinal discomfort, dizziness, headache and the like of people after being drunk, can improve the digestion utilization rate of foods by drunk human bodies, and is especially suitable for people loving drinking wine and drinking wine often to drink.

A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and / or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and / or a salt thereof; and / or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The present invention includes a formulation comprising a physiological salt solution, or an oil-in-water emulsion, or a water immiscible solid phase, and an adjuvant comprising one or more disaccharide derivatives derived from cellobiose, gentiobiose, lactose, lactulose, maltose, melibiose, sucrose or turanose, wherein at least four of the free hydroxyl groups of the disaccharide molecule have been modified such that the disaccharide derivative has: (i) at least 3, but not more than N-1, fatty acid ester groups, wherein each of the fatty acid ester groups is represented by a general formula of —O—C(═O)—(CH2)xCH3 wherein x is between 6 and 14, and (ii) at least one, but no more than N-1 anionic groups, wherein N is the number of hydroxyl groups of the disaccharide from which the derivative is derived and wherein the combined number of fatty acid esters and anionic groups does not exceed N.

The invention provides a rooting liquid for improving the survival rate of evergreen oriental cherry and a preparation method thereof. The rooting liquid comprises: shikonin, calcium nitrate, biotin,chlorogenic acid, wild mint, a rooting agent, gentiobiose, sucrose, amino acid, trace elements, vitamin C, and purified water. The rooting liquid for improving the survival rate of evergreen orientalcherry provided by the invention has reasonable ratio and comprehensive nutrition, contains the trace elements needed by growth of evergreen oriental cherry, can effectively promote the rooting of evergreen oriental cherry cuttings, shorten the rooting time, and improve the rooting rate. The contained trace elements can promote growth, and endow evergreen oriental cherry with deep roots and luxuriant leaves, thus increasing the survival rate of cuttings. The rooting liquid can significantly increase the survival rate of cuttings.

The invention discloses a rapid quantitative detection method for quantitative analysis of multi-components by single marker (QAM) of a pharmaceutical composition containing three-ingredient rhizoma polygonati decoction, and belongs to the technical field of detection and analysis. Specific components of the traditional Chinese medicinal materials in the medicine composition containing the three-component rhizoma polygonati decoction are quantified, three components of the three traditional Chinese medicinal materials are quantified, and determination is carried out under two detection wavelengths in a gradient elution mode. The detection wavelength is as follows: the 5-hydroxymethylfurfural is 270nm, the quercetin-3-O-gentiobioside and the isorhamnetin-3-O-gentiobioside are 330nm, the wave crests are appropriate in size, the 5-hydroxymethylfurfural, the quercetin-3-O-gentiobioside and the isorhamnetin-3-O-gentiobioside do not interfere with each other, the quantitative analysis of multiple components by one marker is realized, and the method is simple, convenient, rapid, accurate, low in cost, high in efficiency and beneficial to market supervision.

A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

The invention discloses a feed capable of improving the quality of crab cream.The feed is prepared from bean pulp, corn meal, minced shrimps, distillers' grains, tea dregs, ootheca mantidis, cynomorium songaricum, modified diatomite, hawthorn fruit, folia perillae acutae and salt; the modified diatomite is prepared from diatomite, alginate oligosaccharide, gentiobiose, nano-sized chitosan, olive oil, compound vitamins, beneficial bacterium powder and distilled water.The feed is reasonable in ingredient matching and rich in nutrition, the feed does not contain harmful substances and is not prone to be pasty in water, the eating habits of crabs are met, the growth speed of the crabs can be increased, the quality taste of the crabs can be improved, the water quality can be improved, the content of harmful substances in the crabs can be lowered, and therefore the quality of the crab cream is guaranteed; the yield of the crab cream can be increased by 10%-12%, and a good economic benefit is achieved.

The invention discloses a preparation method of quercetin-3-gentiobioside, which comprises a crushing step, a reflux extraction step, a decompression concentration step, a centrifugation step, a macroporous resin enrichment step, and a preparation liquid chromatography enrichment step and drying step, the quercetin-3-gentiobioside prepared by the preparation method of the present invention has stable quality and can be added to products such as skin care and beauty treatment as an antioxidant substance, and can also be used as a reference substance to treat the Extracts were quality controlled.

The invention discloses a beta-glucosidase mutant and application of the beta-glucosidase mutant in preparation of gentiooligosaccharide, and belongs to the technical field of enzyme engineering and functional oligosaccharide preparation. According to the invention, site-directed mutagenesis is carried out on a beta-glucosidase gene derived from Thermotoga maristima STB25, and high-efficiency expression of the beta-glucosidase gene in bacillus subtilis is realized. Beta-glucosidase prepared from the mutant is added into a reaction system with 480 g / L glucose as a substrate, reaction is carried out under the conditions that the pH is 6.0 and the temperature is 60 DEG C, and the yield of gentiobiose oligomer can reach 22.12% and is increased by 66.44% compared with that of a wild type. The beta-glucosidase mutant provided by the invention has good acid stability, thermal stability and glucoside conversion capability, can generate gentiobiose through catalysis by taking glucose as a substrate, and has a relatively good application prospect in the field of gentiooligosaccharide production.

The invention relates to a method for relieving pseudo-ginseng autotoxicity injuries by adding exogenous gentiobiose, belonging to the technical field of plant protection. The technical scheme is as follows: carrying out seed soaking treatment on pseudo-ginseng seeds by using a 1.0-50.0 mg / L gentiobiose solution; or dissolving gentiobiose in seed coating Celest to prepare a 5.0-500.0 mg / L gentiobiose seed coating, and carrying out coating treatment on the pseudo-ginseng seeds according to the agent:seed mass ratio of 1:50; or carrying out root soaking treatment on seeds and seedlings by using a 1.0-50.0 mg / L gentiobiose solution. The exogenous antioxidant is added to enhance the resistance of pseudo-ginseng, lower the cell injuries caused by autotoxic substances in the pseudo-ginseng growth process so as to increase the root rot damage probability, and reduce the consumption of pesticides; and thus, the method is an ecological safe effective measure for relieving successive cropping obstacles.

The invention relates to a method for preparing oligogentisose by using β-1,6-glucanase and its application, and belongs to the technical field of genetic engineering and fermentation engineering. The present invention first provides a β-1,6-glucanase TcBgn with transglycosylation activity on gentiobiose, which can convert glucose into oligomeric gentian with a low amount of added enzyme sugar, and the conversion rate is high, which can significantly reduce production costs. In the present invention, β-1,6-glucanase and β-glucosidase double enzymes are also compounded to prepare oligosaccharides, and the double-enzyme compounding system is used to convert glucose into gentiobiose and gentiotriose. , the conversion rate is high, and the proportion of gentiotriose in the product is significantly increased. Therefore, the method for preparing oligosaccharide gentisose by β-1,6-glucanase single-enzyme or double-enzyme compounding has good industrial application value.

The invention discloses an extract of an active anti-fatigue part of okra, and a preparation method and application thereof. The extract of the active anti-fatigue part of okra uses total polyphenols as a main active anti-fatigue component, wherein the total polyphenols account for 11.79 to 23.34% of the total mass of the extract of active anti-fatigue part and mainly comprise catechin derivatives and quercetin derivatives, and the quercetin derivatives are mainly composed of quercetin-3-O-gentiobioside, quercetin-3-O-glucose-xyloside, isoquercitrin, quercetin-3-O-malonyl-glucoside, rutin and quercetin. The extract of the active anti-fatigue part of okra provided by the invention can substantially prolong the load swimming time of mice, eliminate lactic acid accumulation after sports, reduce the content of serum urea nitrogen, increase hepatic glycogen and improve the antioxidation capability of mice in a stress state, and has substantial anti-fatigue effect.

The invention belongs to the field of compound drugs, which relates to new drug application of crocin and a derivant thereof, in particular to application of crocin and a derivant in preparing a hypnotic drug and a health product. The crocin is ester formed by combining crocetin2-naphtholum8-sulfoacid with two-molecule gentiobiose with a chemical formula of C44H62O24 and molecular weight of 974.97. An animal experiment proves that the non-rapid eye movement sleep (slow wave sleep) time can be increased to treat insomnia or improve sleep quality, and the sleeping quantity is improved with dosedependent. The crocin and the derivant thereof can be used for preparing sedative-hypnotic drugs and health products and can be prepared into intestinal or non-intestinal combination drugs which comprise granules, tablets, liquid preparations, sustained release agents, medicinal granules, gelatin pearls, capsules or injections.