Method for preparing cape jasmine glycosides standard substance and its analogue
A standard substance, the technology of geniposide, applied in the field of medicine, can solve problems such as unseen geniposide and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Embodiment 1: Preparation process of geniposide and its analogues
[0046] Take 1 kg of gardenia medicinal material, pulverize it, extract 3 times with 8 L of 70% ethanol under reflux, and extract for 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add water to disperse and dissolve, and make the concentration of the aqueous solution 3.00 mg / mL (based on the amount of geniposide ) through 5L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:8, the impurity is removed by water elution of 5 times the resin volume, and 10% ethanol elution of 2 times the resin volume Carry out impurity removal, the impurity removal flow rate is 1.0mL / min, 30% ethanol elutes 3 times the resin volume, the elution flow rate is 2.0mL / min, collects 1~3 times the resin volume 30% ethanol eluate, reclaims the solvent, reduces Press and dry, that is, geniposide and its analogues. The c...
Embodiment 2
[0047] Embodiment 2: Preparation process of geniposide and its analogues
[0048] Take 2 kg of gardenia medicinal material, pulverize it, 15 L of 50% ethanol, reflux extraction 3 times, extract for 1.5 hours each time, recover the solvent, add water to disperse and dissolve the extract, and make the concentration of the aqueous solution 6.00 mg / mL (based on the amount of geniposide), Through 6L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:7, 4 times the resin volume is eluted with water for impurity removal, and 10% ethanol is eluted for 2 times the resin volume for impurity removal , the impurity removal flow rate is 1.0mL / min, 20% ethanol elutes 5 times the resin volume, the elution flow rate is 3.0mL / min, collects 1 to 5 times the resin volume 20% ethanol eluate, recovers the solvent, and dries under reduced pressure. That is geniposide and its analogues. The content of total iridoid glycosi...
Embodiment 3
[0049] Example 3: Preparation process of geniposide and its analogues
[0050] Take 5 kg of gardenia medicinal material, pulverize, 70% ethanol 10L, reflux extraction 2 times, extract 2 hours each time, recover the solvent, add water to disperse and dissolve the extract, so that the concentration of the aqueous solution is 9.00 mg / mL (based on the amount of geniposide), Through 6L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:9, and 3 times the volume of the resin is eluted with water for impurity removal, the impurity removal flow rate is 1.0mL / min, 20% ethanol Elute 6 times the volume of the resin, the elution flow rate is 3.0mL / min, collect 2-5 times the volume of the resin 20% ethanol eluent, recover the solvent, dry under reduced pressure, that is geniposide and its analogues. The content of total iridoid glycosides was determined to be 75.35%, and the content of geniposide was 68.53%.
PUM
Property | Measurement | Unit |
---|---|---|
purity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com