Method for preparing cape jasmine glycosides standard substance and its analogue

A standard substance, the technology of geniposide, applied in the field of medicine, can solve problems such as unseen geniposide and the like

Inactive Publication Date: 2008-01-30
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no patent report related to the preparation of geniposide standard substance and its quality evaluation method

Method used

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  • Method for preparing cape jasmine glycosides standard substance and its analogue
  • Method for preparing cape jasmine glycosides standard substance and its analogue
  • Method for preparing cape jasmine glycosides standard substance and its analogue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Preparation process of geniposide and its analogues

[0046] Take 1 kg of gardenia medicinal material, pulverize it, extract 3 times with 8 L of 70% ethanol under reflux, and extract for 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add water to disperse and dissolve, and make the concentration of the aqueous solution 3.00 mg / mL (based on the amount of geniposide ) through 5L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:8, the impurity is removed by water elution of 5 times the resin volume, and 10% ethanol elution of 2 times the resin volume Carry out impurity removal, the impurity removal flow rate is 1.0mL / min, 30% ethanol elutes 3 times the resin volume, the elution flow rate is 2.0mL / min, collects 1~3 times the resin volume 30% ethanol eluate, reclaims the solvent, reduces Press and dry, that is, geniposide and its analogues. The c...

Embodiment 2

[0047] Embodiment 2: Preparation process of geniposide and its analogues

[0048] Take 2 kg of gardenia medicinal material, pulverize it, 15 L of 50% ethanol, reflux extraction 3 times, extract for 1.5 hours each time, recover the solvent, add water to disperse and dissolve the extract, and make the concentration of the aqueous solution 6.00 mg / mL (based on the amount of geniposide), Through 6L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:7, 4 times the resin volume is eluted with water for impurity removal, and 10% ethanol is eluted for 2 times the resin volume for impurity removal , the impurity removal flow rate is 1.0mL / min, 20% ethanol elutes 5 times the resin volume, the elution flow rate is 3.0mL / min, collects 1 to 5 times the resin volume 20% ethanol eluate, recovers the solvent, and dries under reduced pressure. That is geniposide and its analogues. The content of total iridoid glycosi...

Embodiment 3

[0049] Example 3: Preparation process of geniposide and its analogues

[0050] Take 5 kg of gardenia medicinal material, pulverize, 70% ethanol 10L, reflux extraction 2 times, extract 2 hours each time, recover the solvent, add water to disperse and dissolve the extract, so that the concentration of the aqueous solution is 9.00 mg / mL (based on the amount of geniposide), Through 6L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the diameter-to-height ratio of the resin column is 1:9, and 3 times the volume of the resin is eluted with water for impurity removal, the impurity removal flow rate is 1.0mL / min, 20% ethanol Elute 6 times the volume of the resin, the elution flow rate is 3.0mL / min, collect 2-5 times the volume of the resin 20% ethanol eluent, recover the solvent, dry under reduced pressure, that is geniposide and its analogues. The content of total iridoid glycosides was determined to be 75.35%, and the content of geniposide was 68.53%.

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Abstract

The invention discloses a fructus gardeniae standard substance and a fructus gardeniae analog extracted from traditional Chinese medicine fructus and the preparation methods thereof. The fructus gardeniae analog consists essentially of fructus gardeniae, iso-fructus-gardeniae, dehydroxy fructus gardeniae (geniposide), geniposidic gentiobiose glycoside, wild fructus gardeniae, gardeniae ketoside; scandoside formate, deacetylasperuloside, geniposidic acid, 10-O-acetyl geniposidic glocoside, 6-para-cormaroyl geniposidic gentiobioside glycoside etc. The fructus gardeniae standard substance and the fructus gardeniae analog can be prepared by any of the methods such as solvent extraction, macroporous absorption resin, supercritical fluid extraction, column chromatography, fluid-fluid countercurrent chromatography, or any combination of the methods. The purity of the prepared fructus gardeniae standard substance is 98-100 percent (w / w), and the total percentage contents of iridoid components in the fructus gardeniae analog are 5-100 percent (w / w), wherein, the content of fructus gardeniae account for 5-100 percent (w / w) of the total iridoid contents.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a method for separating and preparing geniposide standard substances and analogues thereof from gardenia jasminoides, a traditional Chinese medicine, and a quality evaluation method for geniposide standard substances and analogues thereof. Background technique [0002] Gardenia is a commonly used traditional Chinese medicine, derived from the dried ripe fruit of Gardenia jasminoide Ellis. Traditional Chinese medicine theory believes that gardenia has the effects of purging fire and relieving restlessness, clearing heat and diuresis, cooling blood and detoxifying, and is mainly used to treat fever and upset, jaundice, red urine, bloody strangury and pain, vomiting of blood heat, red eyes, swelling and pain, fire and sores modern pharmacological studies have shown that gardenia has various effects such as antipyretic, sedative, anti-inflammatory, and anti-microbial, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04A61K36/744A61K31/7048A61P43/00
CPCY02P20/54
Inventor 石任兵刘斌杨帆李更生
Owner 石任兵
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