215 results about "Iridoid Glycosides" patented technology

The present invention relates to medicine technology, and is especially the preparation process of preparing jasminodin with cape jasmine fruit. The preparation process includes soaking or refluxing cape jasmine fruit with water or water solution of ethanol to obtain coarse extract; eluting in macroporous adsorbing resin first with great amount of water and then with 10 % concentration ethanol solution to eliminate strong polar iridoid glycoside components and other impurities; selectively eluting jasminodin with low concentration ethanol solution; and decompression concentrating to obtain high purity jasminodin. The preparation process of the present invention has simple operation, low cost, high product purity, no environmental pollution and high yield, and is suitable for industrial production.

The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening / diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

The invention discloses an alcohol extractable matter of paederia scandens of Rubiaceae plant, which is characterized by the following: extracting iridoid glycosides compound as effective component; displaying obvious analgesic and anti-inflammatory action when the content of paederia scandensin and paederia scandensin acid methyl ester at 10-90% respectively; improving the analgesic and anti-inflammatory action when the content of paederia scandensin and paederia scandensin acid methyl ester at 20-60% respectively; displaying the best analgesic and anti-inflammatory action when the content rate of paederia scandensin and paederia scandensin acid methyl ester at 5: 1-1: 5. The invention has similar analgesic action to opium drug without habituation.

The invention provides a compound extract for tranquillization, infantile eclampsia resistance and digestion promotion, which comprises 5-95% of iridoid glycosides, 1-90% of saponins, 1-90% of terpenes, 1-90% of flavones and 1-90% of volatile oil.

The invention provides high-purity geniposide as well as preparation and clinical application of preparations thereof. Gardenia is a dried and matured fruit of rubiaceae plant gardenia and has liver-protecting, chole-promoting, pressure-reducing, heat-clearing, cold-promoting and diarrhea-relieving functions and the like. The geniposide is a product which is obtained by refining the dried and matured fruit of the rubiaceae plant gardenia by virtue of a high-technology production process and is an iridoid glycoside compound. The geniposide is used for treating cardiovascular and cerebrovascular diseases, hepatic and biliary diseases, diabetes mellitus and the like. At present, a lot of methods for extracting the geniposide and gardenia pigment from the gardenia are reported in documents, but a plenty of problems exit, such as the product contains high impurity content so that the requirements of customers at home and abroad can not be satisfied, the production cost is high, the yield is low, the economic benefits are poor, the methods are not suitable for industrial production, and the like. According to the invention, the problems are solved. The high-purity geniposide is obtained by virtue of extraction and purification of gardenia and is prepared into proper preparations such as tablets, capsules, particles, injecta, powder injections, infusion solutions and the like to be applied to the clinic for treating cardiovascular and cerebrovascular diseases, hepatic and biliary diseases, diabetes mellitus and the like.

The invention provides a method for extracting cornel iridoid glycoside by virtue of ultrasonic-microwave synergetic extraction. The method comprises the following steps: (1) preparing cornus officinalis coarse powder; (2) adding the cornus officinalis coarse powder into a 80% methanol solution in a material-liquid ratio of (1:5)-(1:25), sealing, weighing and determining the weight, connecting a condensing device, and extracting for 3-15 minutes with microwave power of 300-700W and ultrasonic powder of 120-600W to obtain an extract; (3) carrying out suction filtration on the extract to obtain filtrate, keeping the constant volume, filtering, concentrating, drying and purifying to obtain the cornel iridoid glycoside. A method for determining the cornel iridoid glycoside comprises the following steps: a, preparing a mixed reference substance solution; b, preparing a test substance solution; c, determining the contents of morroniside, loganin, sweroside and cornel iridoid glycoside in the mixed reference substance and the test substance; d, respectively calculating the contents of the four glycosides according to the result of liquid chromatographic analysis.

The invention discloses a new medical application of loganin, and in particular relates to an application of the loganin in preparation of medicaments for treating diabetes, wherein the loganin is extracted from dogwood. The method for extracting the loganin comprises the following steps of: (1) performing water extraction and alcohol precipitation on dogwood coarse powder to obtain a dogwood water extraction and alcohol precipitation extracting solution; (2) loading the dogwood water extraction and alcohol precipitation extracting solution into a macroporous adsorption resin column, and performing enrichment to obtain dogwood iridoid glycosides; (3) performing chromatographic separation by using a silica gel column to obtain a loganin flow component; and (4) purifying to obtain a loganin monomeric compound. Pharmacodynamics experimental results prove that the loganin has the functions of reducing the content of blood sugar, serum AGEs and urokinase protein, raising the serum insulin level, relieving pathologic lesions of pancreas, kidney, liver, heart, testis and the like, and obviously improving a 'three more one less' symptom. Therefore, the loganin can be used for preparing the medicaments for treating the diabetes.

The invention relates to a method for extracting and separating more than 90% iridoid glycoside compound monomer from efflorescence snake tongue grass, wherein the iridoid glycoside compound monomer of efflorescence snake tongue grass is E-6-0-coumaricacetate paederoside methyl ester. The method comprises the following steps: (1)putting the disintegrated efflorescence snake tongue grass in the primary alcohol aqueous solution in order to heat, reflux and extract; filtering; getting the concrete (I)by concentrating; adding the water in order to dissolve; filtering; getting the concrete (II)by concentrating; (2)dissolving the concrete (II)with the secondary alcohol aqueous solution; separating with macroreticular resin column; eluting with the tertiary alcohol aqueous solution; concentrating the eluent; getting the primary product; (3)proceeding with chromatography with Sephadex LH-20 column after dissolving the primary product with the fourth alcohol aqueous solution; proceeding with gradient elution with the fifth alcohol solution; concentrating; crystallizing; getting the product E-6-0-coumaricacetate paederoside methyl ester. The method can recycle the column material and solvent, which doesn't pollute the environment, has the low cost, the high product efficient and the easy operation, and is fit for the small-scale extraction separation of the laboratory and the industry extraction separation.

The present invention relates to simultaneous extraction and measurement of iridoid glycosides from dogwood, and is especially process of simultaneously extracting and measuring six kinds of iridoid glycoside compound. Compared with available technology, the present invention has the advantages of simple extraction process, high efficiency, low cost, fast and effective detection, etc.

Disclosed is a pharmaceutical preparation for tranquilizing, liver-preserving, allaying fever and relaxing bowels, which comprises 5-95% of iridoid glycosides, 1-90% of saponins, 1-90% of flavones, 1-90% volatile oils, and 1-90% of alkaloids.

The invention belongs to the field of medicines and particularly relates to a new application of an iridoid glycoside compound, genipin-1-[beta]-D-gentiobioside, and a composition composed by compounding the genipin-1-[beta]-Dgentiobioside with other components as effective components for preparing antiviral drugs, antibacterial drugs, fever alleviating drugs, inflammation resistant drugs and anti-oxidizing drugs. The iridoid glycoside compound and the composition can be used for treating acute respiratory infection, flu, pneumonia, viral hepatitis type B, herpes zoster, herpes viral keratitis, viral myocarditis, Epstein-Barr virus infection, retrovirus infectious diseases and bacterial infectious diseases.

The invention discloses a paederia scandens water extract containing an iridoid glycoside compound. The extract is characterized in that the iridoid glycoside compound contains 8.00-60.0% of paederosidie acid. The invention also discloses a preparation method of the paederia scandens water extract. The method comprises the steps that: (a) a paederia scandens material or decoction pieces are subjected to water extraction, such that an extraction liquid is obtained; (b) the extraction liquid is concentrated; methanol or ethanol is added, or the concentrated liquid is subjected to standing, and the liquid is precipitated; and a supernatant is obtained; (c) the supernatant is concentrated until no alcohol smell is left; the concentrated liquid is subjected to macroporous resin chromatography; elution is carried out sequentially by using water and ethanol of different concentrations; an eluant is collected; ethanol is recovered; and the eluant is subjected to reduced-pressure drying, such that the paederia scandens water extract is obtained; and (d) optionally, ethanol is added to the paederia scandens water extract obtained in the step (c), and filtering, concentrating, and drying are carried out. The paederia scandens water extract provided by the invention can be used for preparing medicines used for treating gout. The preparation method has simple processes, and is suitable for industrialized productions.

The invention discloses a paederia scandens extract. The extract contains iridoid glycoside compounds, and is characterized in that the iridoid glycoside compounds are mainly composed of 20.0-50.0% of paederosidic acid, 2.0-25.0% of paederoside and 1.0-10.0% of paederosidic acid methyl ester. The paederia scandens extract is prepared by the following ways: a) extracting paederia scandens medicinal material by virtue of ethanol to obtain an extracting solution; b) concentrating the extracting solution until no alcohol smell exists, carrying out macro-porous adsorption resin chromatography, eluting with water and 80-90% of ethanol sequentially, collecting eluate, and drying the eluate under reduced pressure to obtain the paederia scandens extract. The paederia scandens extract disclosed by the invention is applicable to the preparation of medicines which are capable of reducing uric acid, and also resisting inflammation and gouty arthritis.

The present invention relates to medicine technology, and is especially the preparation process of preparing total iridoid glycoside with cape jasmine fruit. The preparation process includes soaking or refluxing cape jasmine fruit with water or water solution of ethanol to obtain coarse extract; eluting in macroporous adsorbing resin first with small amount of water and then with low concentration ethanol solution to purify; and decompression concentrating to obtain high purity total iridoid glycoside. The preparation process of the present invention has simple operation, low cost, high product purity, no environmental pollution and high yield, and is suitable for industrial production.

The invention discloses a fevervine extract and applications thereof. The extract contains iridoid glycoside compounds, which are characterized by being composed of the following components in percentage by weight: 20.0 to 50.0% of paederosidic acid, 2.0 to 25.0% of paederoside, and 1.0 to 10.0% of methyl paederosidate. The fevervine extract is prepared by the following steps: (a) using ethanol to extract fevervine herb so as to obtain an extract solution; (b) condensing the extract solution until no ethanol fragrance can be smelled, then carrying out macroporous resin chromatography on the extract solution, eluting the resin by water and ethanol (80-90%) in sequence, collecting the eluents, and drying under reduced pressure so as to obtain the fevervine extract. The invention further provides applications of the fevervine extract in preparation of drugs for treating and preventing kidney damages.

The invention relates to an extract method of Chinese traditional medicinal compound rhizoma coptidis alexipharmic soup active part and an application of the active part in preparing medicament resisting vascular dementia. The extract method disclosed by the invention adopts industrialized separating and refining technologies, such as microporous membrane and macroporous resin absorption, greatly decreases cream yield of the preparation, maximally retains effective components, improves transforming rate of effective components in raw materials and purity of effective components in the preparation. The extracted active part comprises three kinds of known chemical components: alkaloids, flavonoid glycosides and iridoid glycosides respectively, and has a vascular dementia resisting function.

Belonging to the technical field of traditional Chinese medicines, the invention provides a processing principle-based individualized and characteristic quality evaluation method for Gardenia jasminoides Ellis decoction pieces. Based on preliminary revealing of the processing principle of Gardenia jasminoides Ellis, the method conducts quality evaluation to different Gardenia jasminoides Ellis decoction pieces through diterpenoid pigment components with obvious changes, an HPLC (high-performance liquid chromatography) standard fingerprint of iridoid glycoside and representative compound content. While enriching, perfecting and distinguishing quality evaluation methods for different Gardenia jasminoides Ellis decoction pieces, the method of the invention provides the basis for standardized production and clinical application of Gardenia jasminoides Ellis decoction pieces, provides a demonstration for establishing individualized and characteristic quality standards for crude and cooked decoction pieces. In addition, the method provided in the invention can also be used in quality control and evaluation of resources, plantation, identification, preparations, and drug effects etc. in connection with Gardenia jasminoides Ellis medicinal materials.

A process for extracting the iridoid glycoside from common lamiophlomis herb includes reflux extracting in alcohol solution, macroreticular resin adsorpotion, and separation. Said iridoid glycoside can be used as the active staltic component.

The invention discloses a preparation method for extracting and preparing high-purity gardenia blue pigment from gardenia nutshells. The method comprises the following steps that: the gardenia nutshells are extracted to obtain gardenia nutshell extractum, and the extractum is mixed with macroporous absorption resin to be further eluted and purified to obtain an iridoid glycoside compound; after the eluent is concentrated, amino acid and cellulose enzyme liquid are added for direct reacting; after the reaction is finished, ethanol is added in the reacting liquid for separating and drying; and the quick preparation of high-purity gardenia blue is realized.

The invention discloses a fructus gardeniae standard substance and a fructus gardeniae analog extracted from traditional Chinese medicine fructus and the preparation methods thereof. The fructus gardeniae analog consists essentially of fructus gardeniae, iso-fructus-gardeniae, dehydroxy fructus gardeniae (geniposide), geniposidic gentiobiose glycoside, wild fructus gardeniae, gardeniae ketoside; scandoside formate, deacetylasperuloside, geniposidic acid, 10-O-acetyl geniposidic glocoside, 6-para-cormaroyl geniposidic gentiobioside glycoside etc. The fructus gardeniae standard substance and the fructus gardeniae analog can be prepared by any of the methods such as solvent extraction, macroporous absorption resin, supercritical fluid extraction, column chromatography, fluid-fluid countercurrent chromatography, or any combination of the methods. The purity of the prepared fructus gardeniae standard substance is 98-100 percent (w / w), and the total percentage contents of iridoid components in the fructus gardeniae analog are 5-100 percent (w / w), wherein, the content of fructus gardeniae account for 5-100 percent (w / w) of the total iridoid contents.

The invention discloses a nasal drug delivery gel preparation for curing cerebral diseases. Water is used as solvent in the preparation. The preparation includes geniposide or total iridoid glycoside of cape jasmine fruit, poloxamer 407 and poloxamer 188. The phase transformation temperature of the preparation is 25-35 DEG C. Based on the weight of the preparation, the geniposide or total iridoid glycoside of cape jasmine fruit is 1-5%; the poloxamer 407 is 16-18%; the poloxamer 188 is 0-5%; preferably, based on the weight of the preparation, the preparation is composed of 2% of the geniposide, 16% of the poloxamer 407, 2.5% of the poloxamer 188, 0.2% of ethylparaben, 0.9% of sodium chloride and the balance of water; and the phase transformation temperature of the preparation is 25-33 DEG C. According to the preparation disclosed by the invention, the gel preparation has proper phase transformation temperature by adjusting kinds and proportions of gelling matrixes in the preparation; after the gel preparation is sprayed in a nasal cavity in the form of solution so that gel is rapidly formed under a body temperature condition, therefore, the contact time of the geniposide or total iridoid glycoside of cape jasmine fruit and nasal mucosa is increased; and the distribution of drugs in brain tissue is increased.

The invention discloses an effective part of spreading hedyotis herb and a preparation method thereof. The preparation method comprises the following steps of: purifying the effective part of the spreading hedyotis herb, determining the content of total iridoid glycoside and 6-O-p-coumaroyl scandoside methyl ester in the effective part of the spreading hedyotis herb, and further defining effective anti-tumor ingredients in the effective part; the preparation method is beneficial to increase of the stability of products; meanwhile, mice anti-tumor experiments and the contrast between the effective part and a clinically commonly-used anti-tumor medicine, namely, cyclophosphamide, show that the effective part of the spreading hedyotis herb has an obvious anti-tumor effect.

The invention relates to a method for preparing iridoid aglycone. The method comprises that: iridoid glycoside is subjected to enzyme hydrolysis while continuous liquid-liquid extraction is performed, and an ether organic solvent or an alkyl halide organic solvent is adopted to carry out extraction separation on the hydrolysate iridoid aglycone, such that the high yield iridoid aglycone is obtained.

The invention discloses a honeysuckle quality evaluation method. According to the honeysuckle quality evaluation method, chlorogenic acid, galuteolin and six iridoid glycoside ingredients in honeysuckle are determined, wherein the six iridoid glycoside ingredients are loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal. The honeysuckle quality evaluation method disclosed by the invention has the advantages that the iridoid glycoside ingredients in the honeysuckle are included in a honeysuckle quality evaluation system for the first time, six representative iridoid glycoside ingredients in the honeysuckle are selected and determined at the same time for guaranteeing reliability and effectiveness of the quality evaluation method, and a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) is established for the first time and used for detecting the six iridoid glycoside ingredients of different structures, so that reliability and effectiveness of honeysuckle quality evaluation are obviously improved, quality control level of traditional Chinese medicine honeysuckle and related patent medicines is further improved, and the honeysuckle quality evaluation method disclosed by the invention is significant on guidance of clinical application.

The invention discloses a method for extraction and purification of four iridoid glycosides in cornus officinalis. The method comprises the following steps of 1, accurately weighing cornus officinalis, carrying out heating reflux extraction by an ethanol aqueous solution having a volume concentration of 40-90% for 1-4h, wherein the weight of the ethanol aqueous solution is 5-15 times the weight of the weighed cornus officinalis, carrying out filtration, carrying out extraction 2-4 times, and merging the filtrate, and 2, carrying out purification of the merged filtrate by a mixed macroporous resin comprising HPD300, LSA30 and SP825, carrying out elution by 1-4BV of an ethanol aqueous solution having a volume concentration of 20-80% at an elution flow rate of 1-3BV / h, collecting the eluent, and carrying out condensation to obtain a mixture of four iridoid glycosides. The method has the advantages that 1, processes are simple and safe; a process is short; a cost is low; and industrial production is convenient; and 2, through extraction and purification by the mixed resins, high-purity loganin, swertiamarin, morroniside and cornuside are obtained.

The invention relates to a preparation method for a honeysuckle extract. The method comprises the steps as follows: firstly, ethanol extracting; secondly, vacuum concentrating; thirdly, pH value regulating; fourthly, macroporous resin purifying; fifthly, concentrating, drying and the like. The technology of the method is simple and easy to operate; as the macroporous resin can be recycled, the method is low in manufacturing cost and small in environmental pollution; the ethanol solution, adopted as an extracting and eluting solvent, is high in safety, cheap and easy to obtain, and low in production cost; as the obtained honeysuckle extract and the honeysuckle have basically identical ingredients, namely compounds such as an organic acid, iridoid glycoside, and flavone, an active substance group is wholly transferred; under the situation that invalid substances are removed effectively, the recycling rate of the organic acid, the iridoid glycoside, and the flavone can reach higher than 80%, and the resemblance of the extract finger-print before and after the macroporous resin purification can reach over 0.91.

The invention belongs to the technical field of medicines and discloses a preparation method of a rehmannia iridoid glycoside extractive. In order to increase the extracting rate of the reparation method of rehmannia iridoid glycoside extractive, the method comprises the following steps: firstly performing low temperature tissue fragmentation flash extraction by taking 20% to 50% by mass percentage of methanol or ethanol as a solvent; separating and removing impurities by combination application of polar and non-polar macroporous adsorption resin, and then preparing by using a high-performanceliquid-phase method; and performing low temperature vacuum drying to obtain a dry extractive. For the prepared dry extractive, the content of cycloenethers is 60%-80% (w / w) based on the total contents of catalpol, rehmannioside A and D, and leonuride. The method disclosed by the invention is better than a conventional extraction process, is conducive to development and utilization of rehmannia.

The invention relates to a method for improving the active ingredients of honeysuckle flowers, in particular to a method for improving the active ingredients of honeysuckle flower by a photoenzymaticinduction technology. The method comprises the following sequential steps of (1) taking the honeysuckle flowers; (2) performing -20 DEG C freezing; (3) performing alternate illumination by UV-A being320 to 400nm, UV-B with the wave length being 280 to 320nm and UV-C with the wave length being 200 to 280nm. The method has the advantages that the contents of caffeoyl quinic acid and iridoid glycoside of the active ingredients of the honeysuckle flowers can be obviously increased; the ultraviolet treatment time is greatly shortened; the environment-friendly effect is achieved; meanwhile, small experiments prove that the method is applicable to large-scale industrial production.

The invention discloses a process for preparing Chinese herbal slices of dogwood fruit pulp prepared for decoction, which comprises the following steps: taking crude dogwood fruit pulp; adding water of 0.2 to 4 times by weight; moistening for 1 to 3h, heating to 80 to 100DEG C and boiling for 30min to 6h and then cooling; taking out liquid medicine until the liquid medicine is completely absorbed for later use; adding yellow wine accounting for 20 to 30 percent of the boiled dogwood fruit pulp by weight and uniformly stirring; and heating and steaming by spacing out water for 0.5 to 4h and drying to obtain the Chinese herbal slices of dogwood fruit pulp prepared for decoction. According to the process for preparing the Chinese herbal slices of dogwood fruit pulp prepared for decoction, provided by the invention, plenty of experiment researches are carried out on the basis of the traditional process for preparing the dogwood fruit pulp; and the process of steaming before boiling is applied to the process for preparation, the contents of active components in the dogwood fruit pulp, such as iridoid glycoside, polyose, and the like can be improved, so that the processed dogwood fruit pulp product has better clinical curative effect, which provides stronger scientific basis for the novel process for preparing the Chinese herbal slices of dogwood fruit pulp prepared for decoction. The invention has broad application prospect.

The invention relates to the use of fevervine iridoid total glycoside or the composition of the effective component thereof in preparing medicines for treating pains and inflammation, and belongs to the field of the medical use of the effective component of Chinese medicine. The invention uses the fevervine iridoid total glycoside to prepare analgesics, overcomes disadvantages of strong side effects of the prior Western medicine therapy, etc., and provides the effective component extracted from a single medicine with little side effects as the ideal analgesics. The sources of the raw materials of the fevervine are rich, the invention develops the medicinal prospect for the fevervine iridoid total glycoside, and relative new medicine development can be performed on the basis of the invention.