Preparation method of rehmannia iridoid glycoside extractive

A technology of rehmanniae iridoid glycosides and iridoid glycosides is applied in the directions of medical preparations, pharmaceutical formulations, plant raw materials and the like containing active ingredients, and can solve the problems of low extract content, unfavorable development and utilization of Rehmannia glutinosa, There are no problems such as ingredient content standards for extracts

Active Publication Date: 2018-05-08
THE CHINESE TRADITIONAL HERB MEDICINE RES INST HENAN PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Rehmannia glutinosa extract involved is obtained by water extraction, alcohol extraction, water extraction and alcohol refining, or preparation of water extract by XDA-1 macroporous adsorption resin. Standard, among which the name of rehmannia iridoid glycosides extract is only determined by catalpol single component as the reference substance, and the content of the extract is low, which is not conducive to the development and utilization of rehmannia glutinosa

Method used

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  • Preparation method of rehmannia iridoid glycoside extractive
  • Preparation method of rehmannia iridoid glycoside extractive
  • Preparation method of rehmannia iridoid glycoside extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] ⑴Extraction: 10kg fresh rehmannia medicinal materials were selected and cut into pieces, put in a pulverizer and added 30% by mass methanol solution, the tissue was broken and flashed at room temperature for 2 to 3 times, stirred evenly, and placed in a sealed container for cold soaking 2 to 4 hours. The amount of solvent added and the volume weight of medicinal materials are 10:1.

[0025] ⑵ Filtration: Centrifuge to get the extract, further suction and filter, and set aside.

[0026] (3) Concentration: Evaporate the extract obtained in step (2) at room temperature under reduced pressure with a thin film to recover the solvent, and then concentrate it with a rotary evaporator until it is alcohol-free to obtain a concentrated solution for future use.

[0027] (4) Removal of impurities: the concentrated solution obtained in step (3) is decolorized and purified by activated carbon column chromatography, washed with water to remove impurities, gradient eluted with methano...

Embodiment 2

[0032] ⑴Extraction: 10kg of fresh rehmannia glutinosa was selected and cut into blocks, put in a pulverizer and added with 50% ethanol solution by mass percentage, the tissue was crushed and flashed 2 to 3 times at room temperature, stirred evenly, and placed in a sealed container to cool Soak for 2 to 4 hours to extract. The volume-to-weight ratio of the amount of solvent added to the medicinal material is 10:1.

[0033] ⑵ Filtration: Centrifuge to get the extract, further suction and filter, and set aside.

[0034] (3) Concentration: Evaporate the extract obtained in step (2) with a thin film under reduced pressure to recover the solvent, and then concentrate it with a rotary evaporator until it is alcohol-free to obtain a concentrated solution for future use.

[0035] (4) Impurity removal: The concentrated solution obtained in step (3) is decolorized and purified by activated carbon column chromatography, washed with water to remove impurities, gradient eluted with methano...

Embodiment 3

[0040] ⑴Extraction: 10kg of fresh rehmannia glutinosa was selected and cut into blocks, put in a pulverizer and added with 50% ethanol solution by mass percentage, the tissue was crushed and flashed 2 to 3 times at room temperature, stirred evenly, and placed in a sealed container to cool Soak for 2 to 4 hours to extract. The volume-to-weight ratio of the amount of solvent added to the medicinal material is 10:1.

[0041] ⑵ Filtration: Centrifuge to get the extract, further suction and filter, and set aside.

[0042] (3) Concentration: Evaporate the extract obtained in step (2) with a thin film under reduced pressure to recover the solvent, and then concentrate it with a rotary evaporator until it is alcohol-free to obtain a concentrated solution for future use.

[0043] (4) Impurity removal: The concentrated solution obtained in step (3) is decolorized and purified by activated carbon column chromatography, washed with water to remove impurities, gradient eluted with methano...

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Abstract

The invention belongs to the technical field of medicines and discloses a preparation method of a rehmannia iridoid glycoside extractive. In order to increase the extracting rate of the reparation method of rehmannia iridoid glycoside extractive, the method comprises the following steps: firstly performing low temperature tissue fragmentation flash extraction by taking 20% to 50% by mass percentage of methanol or ethanol as a solvent; separating and removing impurities by combination application of polar and non-polar macroporous adsorption resin, and then preparing by using a high-performanceliquid-phase method; and performing low temperature vacuum drying to obtain a dry extractive. For the prepared dry extractive, the content of cycloenethers is 60%-80% (w/w) based on the total contents of catalpol, rehmannioside A and D, and leonuride. The method disclosed by the invention is better than a conventional extraction process, is conducive to development and utilization of rehmannia.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for separating and preparing iridoid glycoside extracts from Chinese medicine rehmannia glutinosa. Background technique [0002] Rehmannia glutinosa is the fresh or dried tuber root of Rehmannia glutinosa Libosch. and its processed products. It is one of the famous "four pregnant medicines" in my country. It is a traditional Chinese medicine with high medicinal value for nourishing yin and nourishing blood. It has a wide range of activities and functions. have certain effects. [0003] The chemical composition of Rehmannia glutinosa is mainly glycosides, among which iridoid glycosides are the main ones. Pharmacological studies have shown that iridoid glycosides are the main active ingredients. More than 20 kinds of glycosides have been isolated and identified from fresh and dried rehmannia glutinosa: catalpol, dihydrocatalpol, acetyl catalpol, motherwort g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/804
CPCA61K36/804A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 王慧森刘明李更生吕杨周倩
Owner THE CHINESE TRADITIONAL HERB MEDICINE RES INST HENAN PROV
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