106 results about "Catalpol" patented technology

A method of treating non-insulin dependent diabetes mellitus comprising the step of providing a composition comprising a predetermined amount of berberine and a predetermined amount of catalpol, wherein said berberine and said catalpol are major active ingredients of said composition. The composition may further comprise a predetermined amount of oleanolic acid as another active ingredient. The berberine is obtained from natural herbs selected from the group of Berberis, Chelidonium, Stephniz, Coptis, Phellodendron, and Ziziphus, the catalpol is obtained from natural herbs selected from the group of Rehmannia, Verbascum, Panulownia, Glubularia and Adonis, and the oleanolic acid is obtained from natural herbs selected from the group of Olea, Swertia, Astrantia, Lonicera and Beta.

This invention provides a method for separating and extracting high-purity stachyose product from Rehmannia glutinosa and Stachys sieboldii. This invention also provides a method for continuously separating and extracting stachyose and catalpol from Rehmannia glutinosa. The method comprises: separating and purifying the extract by passing through continuous pressure-filtration, microfiltration, ultrafine filtration and nanofiltration membranes, and active carbon column, macroporous adsorption resin column, cation exchange resin column, and anion exchange resin column chromatography to obtain stachyose and catalpol. The method has scuh advantages as increased product quality, low wnwegy consumption, and increased raw material utility.

The present invention relates to a composition comprising an extract of Pseudolysimachion genus plant, and the catalpol derivatives isolated therefrom having anti-inflammatory, antiallergic and anti-asthmatic activity. The extractof Pseudolysimachion genus plantand the catalpol derivatives isolated therefrom shows potent suppressing effect on elevated IgE, IL-4 and IL- 13 levels and eosinophilia in the plasma and BALF, and mucus overproduction in the lung tissues in an OVA-induced asthmatic mouse model. Therefore, it can be used as the therapeutics or functional health food for treating and preventing inflammatory, allergic and asthmatic disease.

The invention relates to a method for preparing catalpol, which has simple operation, light pollution and less equipment investment. The method comprises the following processing steps of: taking fresh roots of rehmannia root; adding methanol of which the volume is 5 to 10 times weight of the fresh roots; refluxing for 2 to 4 times, and taking 0.5 to 2 hours for each refluxing; merging backflow liquid; filtering the liquid; recovering the methanol at reduced pressure and condensing the liquid; adding isopyknic water saturation butarol to extract for 3 to 8 times; merging a butarol layer; recovering the butarol at reduced pressure until the butarol is exhausted; adsorbing the butarol with a macroporous adsorbent resin column; carrying out water washing to remove impurities; carrying out elution with 5 to 30 percent ethanol; collecting eluent of which the volume is 3 to 8 times volume of the column; concentrating and drying the eluent; adding the eluent to a silica gel chromatographic column by a dry method; carrying out elution with chloroform-methanol-water (6:4:1); making the eluent in each column volume as one fluid part; collecting 3rd to 6th fluid parts; merging the fluid parts; recovering the solvent and concentrating the fluid parts; and adding anhydrous ethanol to crystallize. The catalpol prepared by the method has high product purity and is easy to realize industrialization expansion.

A method of restoring insulin beta cells in a living object to lower a plasma sugar level comprising the step of providing a composition of berberine and catalpol as the two major active ingredients of said composition. The composition is preferably include oleanolic acid and taurine as active ingredients. The composition is pre-made into a single dosage unit. The single dosage contains active ingredients within a range between 1 to 300 mg / kg / dl for a single intake and is in a form of packet powders, solution in vials, ampoules or pre-filled syringes, capsule, tablet, draught in water, syrup and / or cachets. The berberine is obtained from natural herbs Berberis, Chelidonium, Stephniz, Coptis, Phellodendron, and / or Ziziphus, the catalpol is obtained from natural herbs Rehmannia, Verbascum, Panulownia, Glubularia and / or Adonis. The oleanolic acid is obtained from natural herbs Olea, Swertia, Astrantia, Lonicera and / or Beta.

The invention relates to a method for preparing catalpol and polysaccharide of rehmannia glutinosa libosch from fresh rehmannia glutinosa libosch. In the process, fresh rehmannia glutinosa libosch serving as a raw material is sliced, alcohol extraction of catalpol is performed first, then water extraction of the polysaccharide is performed, alcohol precipitation is performed, the polysaccharide is purified by nano filtration, and the catalpol is obtained by macroporous resin absorption and medium-pressure silica gel column separation. When the method is used for producing the catalpol and the polysaccharide of rehmannia glutinosa libosch, the raw material utilization rate is high; the cost is low; and the process is simple.

The invention belongs to the technical field of medicines and discloses a preparation method of a rehmannia iridoid glycoside extractive. In order to increase the extracting rate of the reparation method of rehmannia iridoid glycoside extractive, the method comprises the following steps: firstly performing low temperature tissue fragmentation flash extraction by taking 20% to 50% by mass percentage of methanol or ethanol as a solvent; separating and removing impurities by combination application of polar and non-polar macroporous adsorption resin, and then preparing by using a high-performanceliquid-phase method; and performing low temperature vacuum drying to obtain a dry extractive. For the prepared dry extractive, the content of cycloenethers is 60%-80% (w / w) based on the total contents of catalpol, rehmannioside A and D, and leonuride. The method disclosed by the invention is better than a conventional extraction process, is conducive to development and utilization of rehmannia.

The invention discloses an alcohol-group leather sterilization mildewcide comprising the following components: ethanol, N- octyl-group isothiazolinone, dodecyl dimethyl benzyl ammonium bromide, polyoxyethylene sorbitan fatty acid ester (T-80), methylparaben, propylben and deionized water. The components of the alcohol-group leather sterilization mildewcide are added into a stirrer to be uniformly stirred and dissolved and than canned as a finished product of the alcohol-group leather sterilization mildewcide. The alcohol-group leather sterilization mildewcide can fast inhibit the synthesis of protein, solidify thallus and inhibit the synthesis of fungus ergot catalpol to inactivate mycete , has good compounding property and enhances the bactericidal and mildewproof effect. The finished product manufactured by using the alcohol-group leather sterilization mildewcide to process leather has safe and reliable use without irritation on skin; and in addition, the alcohol-group leather sterilization mildewcide is suitable for the bacteriocidal action and the mildew prevention of the leather.

The invention relates to the field of medicine preparation, in particular to a catalpol preparation method, which solves the low conversion rate, low yield and low product quality problems in the preparation of catalpol in the prior art and adopts a process that comprises: washing raw materials, slicing and performing enzymolysis; adding ethanol, performing homogenate extraction for 2 or more times at normal temperature in a homogenate extraction device; mixing homogenate, filtering out slag, collecting filtrate, concentrating the filtrate under reduced pressure, adding water to dilute thick extract, adding the diluted extract into macroporous resin, eluting, and concentrating the eluent under reduced pressure to obtain extract; and adding sample mixing silica gel, uniformly stirring, passing the stirred product through a silica gel column, concentrating eluent under reduced pressure, and recrystallizing in methanol to obtain pure catalpol. The method of the invention greatly improves production efficiency and conversion rate, promotes more effective material in the raw material to be converted into catalpol, further improves product quality, improves product purity and is suitable for industrial production.

The invention relates to a method for extracting catalpol from rehmannia stem, which belongs to the medicine technical field. The method comprises the following steps: 1) rehmannia stem and water are heated for backflow extraction, an extract is performed pressure reduction and concentration to obtain a concentrate A; 2) 95% of ethanol is added in the concentrate A, standing is carried out for overnight, a supernate is performed pressure reduction and concentration to obtain a concentrate B; 3) a concentrate B is led to a XAD-16 macroporous resin column, elution is carried out by water, then elution is carried out by ethanol, an ethanol eluate is collected, the pressure reduction and concentration are carried out to obtain a stiff paste; 4) silica gel is added in the stiff paste, dried and a silica gel column is added, elution is carried out by ethyl acetate / methanol according to ratio of 4:1, a target fraction is collected, the pressure reduction, concentration and drying processes are carried out; 5) recrystallization is carried out on a dried object, the processes of filtering and drying are carried out to obtain the catalpol pure product. Compared with the prior art, the method of the invention has the characteristics of strong industrialization operationality, high extraction efficiency, resource preservation and environmental protection.

The invention discloses a method for extracting catalpol from traditional Chinese medicine rehmannia root, belonging to the technical field of biomedicines. The method comprises the following steps: 1) taking homogenate of fresh rehmannia root and water, further adding methanol, stirring and then, filtering to obtain filtrate; 2) further extracting filter residue with methanol solution once, merging filtrate obtained by filtering twice and concentrating to obtain viscous liquid; 3) adding ethanol solution into the liquid, stirring and pouring out obtained clear liquid when no precipitate is generated any more; concentrating the obtained clear liquid till no alcohol exists so as to separate out pigment contained in rehmannia root from solution; 4) filtering insoluble, extracting water phase with n-butanol twice, merging n-butanol phase and removing solvent by reduced pressure evaporation to obtain grey white crystals; 5) recrystallizing the crystals with water once to obtain white crystals, namely catalpol. The extraction method disclosed by the invention has the advantages of simple process, extensive sources of raw material traditional Chinese medicine rehmannia root, low cost and environment-friendly extraction preparation process and opens a broad market for developing novel catalpol health care products and medicaments.

The invention relates to a medical prescription of catalpol freezing-drying powder for injection and a preparation method thereof. The invention is characterized in that the medical prescription of the catalpol freezing-drying powder for injection comprises 1 to 1000 mg of catalpol, 50mg to 2000mg of mannite, 100mg to 4000mg of glycine, 25mg to 1000mg of fucose, and 10% of sodium hydroxide solution right amount (pH 6.8-7.0). The invention also contains the medicinal application of the catalpol freezing-drying powder for injection which is used for treating clinical ischemia cerebral disease. The invention also comprises accessories used in the prescription which are used for preparing the catalpol and the freezing-drying powder for injection of the composition which is prepared by the catalop and other medicines according to different mixing ratios and is used for treating the ischemia cerebral disease. The invention aims at providing the traditional Chinese medicine active ingredient medicinal preparation. The preparation can permeate the blood brain barrier, alleviates the injury degree of the cerebral ischemia, increases the tolerance capability of the ischemia and oxygen-poor of brain tissues, promotes the newborn and the mature of brain microvascular, protects neurovascular units, promotes the neuron axon and dendrite growth, strengthens the synapse reconstruction, improves the back neutral behaviors of the cerebral ischemia, increases the neutral function score, and promotes the motor function recovery of affected limbs after the cerebral ischemia.

The invention discloses a method for drying rehmannia through nitrogen regulating hot air. The method includes the steps that ultrasonic preprocessing is carried out on the rehmannia firstly; and then nitrogen regulating hot air drying is carried out. According to the method for drying the rehmannia through the nitrogen regulating hot air, ultrasonic preprocessing is combined with the nitrogen regulating hot air drying to dry the rehmannia; advantage complementing and the coordination effect of a plurality of processing modes are achieved; catalpol losses in the rehmannia drying process are slowed down; the dry rehmannia quality is improved; meanwhile, the drying resistance is reduced; the drying efficiency is improved; the drying time is shortened. By means of the method for drying the rehmannia, the time used for achieving the dry rehmannia safety storage moisture content which is 0.15g / g in dry basis ranges from 130 min to 510 min; the dry rehmannia catalpol content ranges from 2.42% to 2.98%; the drying time is short; the catalpol content is high; the problems that traditional drying time for drying the rehmannia through the hot air is long, and the drying quality is not high are solved; rapid and quality-retaining drying of the rehmannia is achieved.

The invention provides a pharmaceutical composition for treating diabetes and application thereof. The pharmaceutical composition is mainly prepared from the following raw materials in parts by weight: 1 part of coptis chinensis and 0.1-20 parts of fresh rehmannia; the pharmaceutical composition is prepared by the following steps: extracting the coptis chinensis through steps of performing ethanol reflux extraction, and concentrating and drying an extracting solution to obtain a coptis chinensis extract; extracting the fresh rehmannia through steps of performing ethanol reflux extraction, and concentrating and drying an extracting solution to obtain a fresh rehmannia extract; then mixing the coptis chinensis extract with the fresh rehmannia extract. The invention provides application of the pharmaceutical composition in preparing a medicine for treating the diabetes as well. The pharmaceutical composition disclosed by the invention has a significant advantage in the transfer rate of such main active ingredients as catalpol, stachyose and berberine and the like compared with Qianjin optis chinensis pills prepared by a conventional process; moreover, pharmacological experiments prove that the medicine disclosed by the invention has significant pharmacological effects of regulating fat and reducing sugar, and the pharmacological effects are remarkably better than that of a conventional process product.

The invention discloses an efficient, simple and convenient method for preparing cyanidenon-7-O-beta-D-glucuronide. The efficient, simple and convenient method comprises the following steps of: a. smashing an irexis sonchifolia raw material, soaking in 5-10 times amount of saturated lime water for 2-3 times, mixing extracting solutions, filtering, carrying out molecule trapping sequentially by an ultrafiltration membrane and a nanofiltration membrane, adjusting pH of trapped liquid to 3-5, absorbing the trapped liquid by polyamide resin, carrying out gradient elution by an ethanol solution, and concentrating eluent to obtain a concentration solution; and b. purifying the concentration solution by adopting high-speed counter-current chromatography, carrying out online monitoring by an ultraviolet detector, collecting a target component according to an atlas, recovering the reagent, and drying under reduced pressure to obtain the catalpol. The method for preparing the cyanidenon-7-O-beta-D-glucuronide has the advantages that the process operation is simple, the product yield is high, and the industrial amplification is easy.

The invention relates to application of catalpol as a non-enzymatic glycosylation end product inhibitor. The catalpol is an iridoid glucoside compound and mainly exists in plants such as radix rehmanniae. The catalpol can prevent NO activation, can be used for preventing and treating related diseases such as diabetic complication caused by AGE disorder, Alzheimer's disease and atherosclerosis and has certain blood sugar reducing and blood lipid reducing effects.

The invention relates to the technical field of extraction of active substances in Chinese medicinal herbs and particularly discloses a method for extracting polysaccharide and catalpol in dried rehmannia root. The method includes the following steps: firstly, performing sample pretreatment, and drying the dried rehmannia root to constant weight prior to smashing and sieving; secondly, for extraction, uniformly mixing the treated dried rehmannia root and distilled water with the solid-to-liquid ratio being 1:(8-12)g / ml, heating to the temperature of 90-100 DEG C, and extracting for 1-2 hours for two times to three times, and combining extracting solution; thirdly, concentrating the extracting solution and drying to obtain finished products. Further, in the third step, the extracting solution is concentrated to a specific weight of 1.10g / ml and is added with 5% of maltodextrin, inlet air temperature of a spray dryer is 150+ / -2 DEG C, and outlet air temperature is 80+ / -2 DEG C. Yield of the polysaccharide and catalpol in prepared polysaccharide and catalpol mixture is high, and the method has the advantages of simple techniques, less extraction time and low cost and is suitable for industrial production.

The invention discloses a novel application of catalpol. The novel application is an application of catalpol in preparation of drugs or healthcare food for treating hyperthyroidism or hyperthyroid heart disease related with hyperthyroidism. It has been proved by experiment results that catalpol can improve the appearance and behavior of hyperthyroidism mice, is tend to increase the weight of mice, can reduce the oxygen consumption and slow down the heart rate, has a prominent reducing effect on T3, FT3, and FT4, and has a prominent reducing effect on the whole heart weight index and left ventricle weight index.

The invention provides a method for extracting and separating effective components of rehmannia glutinosa Libosch and extracted catalpol and polysaccharides. A plurality of components of the rehmanniaglutinosa Libosch are extracted, which gets rid of the limitation of extraction and separation of a single target, greatly improves the utilization rate of the rehmannia glutinosa Libosch and finallyachieves multi-target co-production of the catalpol and the polysaccharides. The method is simple in operation and low in equipment requirements, improves the yield by 3-5 times by using enzymatic hydrolysis, saves important raw materials, and is easy in industrial mass production. The extraction and separation method obtains the catalpol monomer and the polysaccharide monomer, wherein the purityof the catalpol monomer is greater than or equal to 98%.

The invention belongs to the technical field of medicinal chemistry and medicine, and discloses a catalpol derivative or pharmaceutically acceptable salts thereof, as well as a preparation method and application of the catalpol derivative or the pharmaceutically acceptable salts thereof. The structural formula of the catalpol derivative or the pharmaceutically acceptable salts thereof is shown as the formula (I), wherein R1 is a hydrogen atom or a C1-C4 branched chain or branched paraffin. The compounds or the pharmaceutical salts thereof have wide pharmacologic actions.

The invention provides a method for determining three active components in a medicinal preparation, which comprises the following steps: adding water to dissolve the medicinal preparation, fixing the volume, performing shaking uniformly, performing filtering, detecting the obtained test solution by high performance liquid chromatography under double detection wavelengths, and determining the contents of the three active components in the test solution: catalpol, rehmannioside D and regallily glycoside B. The method for determining the three active components in the medicinal preparation is accurate, reliable, simple, convenient, rapid and good in stability and repeatability, provides a basis for systematic evaluation and quality control of the pharmaceutical preparation, provides a research basis for reasonable development and application of the pharmaceutical preparation, can more comprehensively guarantee the quality of the medicinal preparation, improves the safety and the reliability of the medicine are improved, and has a wide application prospect.

The invention belongs to the technical field of medicinal material processing, and particularly relates to a method for processing rehmannia root, which aims to solve the technical problem that the different steaming degrees and long cooling and moisturizing time of rehmannia root with different sizes and thicknesses affect the effect of prepared rehmannia root. The technical scheme is that the method for processing rehmannia root comprises the following steps of: cleaning rehmannia root, cutting rehmannia root into slices which are 1 to 3 centimeters thick, putting the slices into a sterilization cabinet, sterilizing at the temperature of between 110 and 130 DEG C for 0.5 to 1.5 hours, taking the sterilized slices out, and drying. Due to the adoption of the technical scheme, the time of processing rehmannia root is shortened, the processing cost is reduced, the content of stachyose and catalpol which serve as main ingredients in rehmannia root is increased, and the medicinal effect of rehmannia root is enhanced simultaneously.

The invention discloses a method for producing catalpol by utilizing suspension-cultured rehmannia glutinosa cambium stem cells. The method specifically comprises the following steps: taking rehmanniaglutinosa plant stems as explants and establishing a rehmannia glutinosa cambium stem cell culture system capable of stable growth through induced culture, isolation culture and subculture; then adding a combined inductor beta-CD and methyl jasmonate in a bioreactor for suspension culture and refining through ultrasound extraction, macro-porous resin absorption and a high-performance liquid-phasepreparation column to obtain the high-purity hinokitiol. The method disclosed by the invention has the benefits that the utilized plant stem cell culture system has the excellent characteristics of synchronous growth, stable genetic character, high growth speed, stable catalpol synthesis and the like and provides a novel method for catalpol preparation as a raw material for catalpol preparation;in addition, compared with a chemical synthesis method, the method for producing the catalpol by utilizing a bioreactor culture technology combined with a plant stem cell culture technology has the advantages of being short in production period, high in safety, low in cost, high in yield, low in pollution and the like.

The invention discloses a novel application of catalpol, which belongs to the medicine technical field. The invention concretely relates to the application of catalpol in the preparation of medicines for treating cerebral arterial thrombosis sequelae, and the application of the catalpol in the preparation of medicines for promoting recovery of touch neurologic function of cerebral arterial thrombosis sequelae.

The invention belongs to the technical field of the medicine and chemical industry, discloses a natural catalpol derivative and a novel modified catalpol derivative derived from catalposide and picroside II, and provides a preparation method of the derivative and application of the derivative in the preparation of anti-inflammatory drugs. The structure of the natural catalpol derivative is shown in a formula (I) or a formula (II), and the structure of the novel modified catalpol derivative derived from the catalposide and the picroside II is shown in a formula (III) or a formula (IV). Experimental results show that compounds of which the structures are shown in formula (I), formula (II), formula (III) and formula (IV) or hydrates of the compounds, pharmaceutically acceptable salt, tautomers, stereisomers and precursor compounds have a significant inhibiting effect on 8-hydroxytridine DNA glycosylase 1 (8-hydroxytridine DNA glycosylase 1, OGG1), and has wide application space in the preparation of the anti-inflammatory drugs and antitumor drugs.

The invention discloses nutritional and healthcare honey-fried radix rehmanniae. The average content of catalpol in the honey-fried radix rehmanniae is 0.5-0.8%. The average content of verbascoside in the honey-fried radix rehmanniae is 0.03-0.04%. The nutritional and healthcare honey-fried radix rehmanniae has the advantages that the cold nature of raw radix rehmanniae is eliminated, the functions of removing heat to promote salivation, cooling blood and stopping bleeding are reserved, and the blood replenishing, yin nourishing, essence boosting and marrow filling functions of prepared radix rehmanniae are realized; the nutritional and healthcare honey-fried radix rehmanniae has the functions of raw radix rehmanniae and prepared radix rehmanniae at the same time and can improve immunity, regulate blood sugar level, resist tumor, resist inflammation and the like.

The invention discloses a simple and efficient catalpol preparation method. The simple and efficient catalpol preparation method comprises the following steps of: a. smashing fresh radix rehmanniae, juicing, adding the same volume of 95% alcohol to obtained juice, fully stirring, centrifuging, concentrating centrifugate by a nanofiltration membrane, and carrying out spray drying on a concentrated solution to obtain a crude extract; b. adding the crude extract to ethyl acetate and completely dissolving; filtrating, processing filtrate by an aluminium oxide column, purifying a reagent, which is recycled from liquid processed by column, by adopting high-speed counter-current chromatography, carrying out online monitoring by an ultraviolet detector, collecting a target component according to an atlas, recovering the reagent, and drying under reduced pressure to obtain the catalpol. The simple and efficient catalpol preparation method has the advantages that the process operation is simple, the product yield is high, and the industrial amplification is easy.

The invention discloses a novel use of catalpol in preparation of a drug for treating cerebral arterial thrombosis reperfusion injuries and belongs to the technical field of medicines. Catalpol can be used for preparation of a drug for treating cerebral arterial thrombosis reperfusion injuries and especially can be used for preparation of a drug for treating cerebral arterial thrombosis reperfusion injuries and neurologic impairment.

The invention relates to a method for extracting catalpol and in particular relates to a method for ultrasonic extraction on catalpol. The method comprises the following treatment steps: crushing fresh rehmannia roots into 2-10mm; adding distilled water which is 1-2 times of the mass of the treated rehmannia root powder into the treated rehmannia root powder, and further adding a prepared methanolblending liquid (the density is 0.82-0.89g / ml) which is 2-3% by weight of the raw materials; putting the obtained material into an ultrasonic device, further adding a 70-95% ethanol solution which is4-6 times of the weight of the raw materials, performing extraction for 3-4 hours under conditions that the ultrasonic frequency is 40KHz and the temperature is 60-70 DEG C, performing vacuum concentration on the extract, and recycling ethanol so as to obtain a concentrated solution; eluting the obtained concentrated solution by using 80% ethanol on a macroreticular resin column, collecting eluant, controlling the concentration temperature to be less than or equal to 50 DEG C, controlling the vacuum degree to minus 0.05MPa to minus 0.08MPa, and performing drying, so as to obtain catalpol. Themethod provided by the invention is simple and feasible, is high in content of a purification substance, namely the catalpol, is good in reproducibility, and in addition, a resin can be repeatedly used.