35 results about "Catalpol" patented technology

The present invention provides a traditional Chinese medicine composition for clearing heat, removing blood stasis, stopping bleeding and regulating menstruation, and a preparation method thereof, relating to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition of the present invention comprises the following components: catalpol, aucubin, β-sitosterol, emodin, chrysophanol, sanguisorba officinalis extract, Panax notoginseng saponins, septamine, cuttlefish scaly, rubiacin, typhaneuroside, and p-coumaric acid; the components synergistically work together to significantly reduce plasma prothrombin time and plasma recalcification time, and significantly reduce the whole blood viscosity, plasma viscosity, and hematocrit of rats with blood stasis syndrome. The traditional Chinese medicine composition of the present invention is used in the preparation of drugs for menstrual disorders or menstrual bleeding and metrorrhagia, and has a significant therapeutic effect on irregular menstruation, excessive menstrual bleeding, or metrorrhagia.

The invention discloses a heart-protecting granule identification and quality control method based on thin layer chromatography, and belongs to the technical field of medicine detection. The thin-layer chromatography identification method comprises the following steps: identifying astragalus membranaceus by taking astragaloside as a reference substance, identifying wine-treated rhubarb by taking chrysophanol and emodin as reference substances, identifying leech by taking a leech medicinal material as a reference substance, identifying liquorice by taking liquiritin and a liquorice medicinal material as reference substances, and identifying radix rehmanniae by taking catalpol as a reference substance. And identifying salvia miltiorrhiza by taking salvianolic acid B and salvia miltiorrhiza medicinal materials as reference substances, and identifying fructus aurantii by taking naringin and neohesperidin as reference substances. By establishing the thin-layer chromatography identification method of the heart-protecting granules, an exclusive developing solvent and a developing mode are adopted, accurate identification is achieved according to the characteristics of spot colors, fluorescence characteristics and the like of corresponding positions of a test substance chromatography, a reference substance and a reference medicinal material chromatography, and the method is easy and convenient to operate, low in detection cost and high in repeatability and specificity; and quality control can be effectively carried out in the production process of the heart-protecting granules.

This invention discloses a method for preparing Rehmannia glutinosa pulp with high retention of all its components after nine steaming and nine sun-drying processes, belonging to the technical field of deep processing of traditional Chinese medicine and preparation of functional foods. Using Rehmannia glutinosa processed through nine steaming and nine sun-drying processes from authentic Huai Rehmannia glutinosa as raw material, this method addresses industry pain points such as the inability to dry-crush candied Rehmannia glutinosa, low efficiency of traditional extraction methods, easy loss of heat-sensitive active ingredients, and difficulty in retaining all components. It adopts wet pulping instead of dry crushing and pioneers a three-stage progressive cell-wall breaking process of "enzymatic hydrolysis → ultrasound → microwave," achieving a synergistic effect of gentle biological cell-wall breaking, physically enhanced crushing, and low-temperature solubilization. Combined with vacuum low-temperature extraction, post-fermentation with probiotics, and low-temperature concentration, the entire process avoids membrane separation, protein removal, and purification of individual components. The finished product achieves a polysaccharide extraction rate of 90%–96%, a total retention rate of ≥65% for catalpol and rehmannia glycoside D, an increase of over 30% in melanoidin content, a decrease of over 25% in 5-HMF content, and an increase of over 13% in in vitro antioxidant activity. The process is gentle and controllable, suitable for large-scale industrial production.

This invention relates to the field of microbial fermentation technology, specifically a method for preparing Rehmannia glutinosa through simultaneous fermentation with compound bacteria and a laxative effect. This method involves extracting four types of probiotics that meet national safety standards: Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus casei, and Saccharomyces cerevisiae, and then formulating a high-concentration compound bacterial agent according to the live bacteria ratio. The initial fermentation material is adjusted to a material-to-water ratio of 1:5 and a suitable pH is maintained. After autoclaving, the materials are inoculated with bacteria at a weight-to-volume ratio for biological culture. The entire system is placed in a 37°C environment for continuous constant-temperature static co-fermentation. Through specific enzymatic hydrolysis and metabolic pathways of multidimensional bacterial communities, the absolute content of the core active ingredient catalpol and soluble dietary fiber in the final product is significantly increased. This bio-fermentation process effectively eliminates the potential risk of spleen and stomach irritation and damage from traditional Chinese medicinal materials. The resulting dry powder raw material can be widely and safely used in the preparation of microecological functional foods or pharmaceutical preparations targeting constipation symptoms caused by intestinal dryness and fluid deficiency.

The invention discloses a quality control method and application of eyesight-improving and kidney-nourishing tablets, and relates to the technical field of medicines.The control method comprises the steps that firstly, a fingerprint spectrum of the eyesight-improving and kidney-nourishing tablets is established and used for quality detection of the eyesight-improving and kidney-nourishing tablets, and effective components of six medicinal materials in the eyesight-improving and kidney-nourishing tablets can be detected; meanwhile, the content of glucose, salidroside, chlorogenic acid, verbascoside, catalpol, specnuezhenide, rehmannia D, 3, 5-O-dicaffeoylquinic acid, 4, 5-O-dicaffeoylquinic acid, chrysophanol, luteolin, luteoloside, aurantio-obtusin and beta-ecdysterone in a sample can be quantitatively detected. Establishment of the fingerprint spectrum detection method of the eyesight-improving and kidney-nourishing tablets and determination of standard fingerprint spectrum information of the eyesight-improving and kidney-nourishing tablets have important significance on work in the aspects of product quality control, authenticity identification, origin traceability and the like.

This invention discloses the application of catalpol in the preparation of drugs for the prevention and treatment of schizophrenia and the alleviation of the glucose and lipid metabolism side effects of olanzapine. Using a schizophrenia model mouse model induced by the commonly used MK801, the therapeutic effect of catalpol on schizophrenia was investigated, and the therapeutic effect of catalpol on the glucose and lipid metabolism side effects of the commonly used drug olanzapine was investigated through long-term gavage administration. A systematic study of catalpol was conducted from pharmacodynamic, behavioral, and morphological perspectives. The results showed that catalpol effectively improved the behavioral characteristics of the schizophrenia model mice, alleviated negative symptoms and cognitive impairment; catalpol effectively improved olanzapine-induced obesity and lipid metabolism disorders. This provides a theoretical research basis for the development of catalpol into a drug for the treatment of schizophrenia.

The invention discloses a pharmaceutical composition for treating type 2 diabetes mellitus and application of the pharmaceutical composition. The pharmaceutical composition for treating type 2 diabetes mellitus is prepared from atractylenolide I, catalpol, berberine, quercetin, magnolol and nobiletin according to the mass ratio of (2.5 to 7.5): (1 to 3): (5 to 20): (5 to 10): (1 to 10): (1 to 40). According to the research on the pathogenesis of type 2 diabetes mellitus and the active ingredients of traditional Chinese medicines, the active ingredients of the traditional Chinese medicines with a targeted therapeutic mechanism are screened by applying a molecular docking theory, and the composition is self-planned to be composed of various active ingredients of the traditional Chinese medicines based on the traditional Chinese medicine state target theory and clinical Pi-dan prescription application experience, and is explored in the research on the pharmacological mechanism of treating T2DM. Researches find that the pharmaceutical composition has a good T2DM treatment effect, and the action mechanism of the pharmaceutical composition is realized by improving bile acid metabolism, intestinal flora homeostasis and glucose homeostasis of liver-intestinal axes of T2DM rats.

The invention belongs to the technical field of medicine detection, and discloses a fingerprint spectrum detection method of Huangshi Yin-nourishing granules, which comprises the following steps: step 1, taking extract of the Huangshi Yin-nourishing granules, adding methanol, carrying out ultrasonic treatment, fixing the volume by using methanol, and filtering to obtain a test solution; 2, respectively weighing catalpol, salvianic acid A, safflower yellow A, paeoniflorin, salvianolic acid B, harpagoside and cinnamic acid reference substances, adding methanol, and respectively preparing single reference substance solutions; mixing the reference substance solutions to prepare a mixed reference substance solution; 3, respectively sucking the test solution in the step 1 and the reference solution in the step 2, injecting into a high performance liquid chromatograph, and recording a chromatogram; the elution procedure of the liquid chromatography is gradient elution. The detection method is simple and convenient to operate, stable in result and good in reproducibility, and can comprehensively, scientifically and accurately evaluate the quality of the Huangshi Yin-nourishing granules.

The invention relates to the field of traditional Chinese medicines, in particular to an optimization method of a processing technology of dried rehmannia root charcoal, which comprises the following steps of: detecting the contents of catalpol and leonurus glycoside in the dried rehmannia root charcoal at different processing times and different processing temperatures through a single factor experiment; basic data parameters of a raw rehmannia root charcoal extraction process are improved through a combined response curved surface method, and the content change of catalpol and leonuride at different processing temperatures and different time is measured, so that the optimal processing temperature and the optimal processing time are obtained; according to the method, the process is optimized by combining a response surface method and taking the content of catalpol and leonuride in the radix rehmanniae charcoal as indexes, and a theoretical basis is provided for subsequent experiments.

This invention belongs to the field of biomedical technology and discloses the use of catalpol and mesenchymal stem cells in the preparation of drugs for treating liver fibrosis, wherein the concentration of catalpol is 50–200 μM and the concentration of exosomes is 20–50 μg / mL. Simultaneously, this invention constructs a spatially resolved liver fibrosis chip model based on GelMA hydrogel, integrating Kupffer-like cells, hepatic stellate cells, hepatic parenchymal cells, and endothelial cells through a layered sequential seeding strategy, achieving high-precision biomimicry of the liver fibrosis microenvironment. In vitro chip and in vivo animal experiments confirmed that this combined approach can significantly inhibit the expression of collagen synthesis-related genes, improve liver function indicators, and effectively reduce collagen deposition; its anti-fibrotic efficacy is significantly better than the simple superposition of the effects of single components, exhibiting a clear synergistic effect. This invention provides a novel synergistic drug strategy and a highly biomimetic in vitro evaluation platform for the treatment of liver fibrosis.

The invention provides a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of hypoglycemic products, and relates to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials: 4-8 parts of ginseng stem and leaf saponin, 8-16 parts of astragalus polysaccharide, 6-12 parts of astragaloside, 8-16 parts of catalpol, 6-12 parts of rehmannia D, 8-16 parts of verbascoside, 3-5 parts of raspberry polysaccharide, 4-8 parts of ellagic acid, 4-8 parts of pachymaran, 5-10 parts of pachymic acid, 8-16 parts of lycium barbarum polysaccharide, 40-62 parts of schisandra chinensis, 20-40 parts of Chinese yam, 40-62 parts of radix ophiopogonis, 40-62 parts of radix trichosanthis and 40-62 parts of rhizoma alismatis. The traditional Chinese medicine raw materials are pretreated by using the high-voltage pulsed electric field, and then ethanol extraction treatment is performed twice, so that the yield of the traditional Chinese medicine extract is high, and the traditional Chinese medicine composition obtained by mixing the traditional Chinese medicine extract with other active ingredients has an excellent effect of treating type II diabetes mellitus.

The application relates to the technical field of pharmaceutical analysis, and particularly discloses a detection method for simultaneously detecting the contents of catalpol, rehderin D and chlorogenic acid in medicinal liquid. The detection method for simultaneously detecting the contents of catalpol, rehderin D and chlorogenic acid in medicinal liquid adopts high performance liquid chromatography detection; the gradient elution program is as follows: 0-35 min, 1% of methanol and 99% of 0.18wt% phosphoric acid solution; 35-45 min, the methanol is increased from 1% to 5%, and the 0.18wt% phosphoric acid solution is decreased from 99% to 95%; 45-60 min, the water is increased from 0% to 20%, the methanol is increased from 5% to 30%, and the 0.18wt% phosphoric acid solution is decreased from 95% to 50%; 60-90 min, the water is decreased from 20% to 0%, the methanol is increased from 30% to 50%, and the 0.18wt% phosphoric acid solution is 50%. The application can accurately detect the contents of catalpol, rehderin D and chlorogenic acid in medicinal liquid, and control the quality of the medicinal liquid.

The invention discloses a thin-layer identification method for Rehmannia root medicinal materials and a Chinese medicine preparation containing Rehmannia root, and relates to the technical field of Chinese medicine quality control. The thin-layer identification method for the Chinese medicine preparation containing Rehmannia root of the present invention is as follows: (1) taking a sample to be tested, adding a solvent, ultrasonic treatment, filtering, evaporating the filtrate to dryness, dissolving the residue in water, extracting with an organic solvent, combining the organic solvent layers, evaporating to dryness, dissolving the residue in water, applying D101 macroporous resin to the aqueous solution, eluting with water until it is nearly colorless, then eluting with an ethanol solution of pH 2-5, collecting the eluate, evaporating to dryness, dissolving the residue in methanol, and obtaining a test solution; (2) taking a control medicinal material of Rehmannia root to prepare a control medicinal material solution; (3) taking the test solution and the control medicinal material solution and spotting them on a thin-layer plate, developing with a developing agent, drying, developing, and inspecting. The thin-layer identification method of the present invention can not only identify a single-ingredient medicinal material of Rehmannia root, but also can better identify a compound Chinese patent medicine preparation, and can detect at least three components other than catalpol in Rehmannia root, with good separation of each component and no interference.

The invention discloses a method for detecting 10 components in rehmannia and processed products thereof, and belongs to the technical field of traditional Chinese medicine component detection. The invention particularly discloses a method for detecting catalpol, rehmannia D, mannose, fructose, glucose, cane sugar, melibiose, raffinose, manninotriose and stachyose in rehmannia and processed products by using a high performance liquid reversed phase chromatography. The elution gradient and flow velocity are 0-30 min, 30% A, 70% B and 0.3 mL / min; 30-40 min, 30% of A and 70% of B are adopted, and 0.4 mL / min is adopted; 40-60 min, 35% of A and 65% of B are adopted, and 1 mL / min is adopted; according to the detection method disclosed by the invention, the detection limit of catalpol and the detection limit of rehmannia D can be equivalent to the detection limit of a variable-wavelength ultraviolet-visible spectrophotometric detector; meanwhile, according to the detection method disclosed by the invention, each detection substance can meet quantitative requirements; and a foundation is laid for research on synchronous detection of various components in rehmannia, accurate control of medicinal material quality, clarification of a processing mechanism and guarantee of clinical curative effect.

The invention relates to the field of biological medicine, in particular to application of catalpol in preparation of a periprosthetic osteolysis inhibitor, which is helpful for inhibiting aseptic loosening of a prosthesis, can remarkably reduce the occurrence rate of joint replacement postoperative complications and realizes more effective clinical intervention, thereby accelerating rehabilitation of a patient and improving the daily life quality of the patient.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The application discloses a heart-protecting granule identification and quality control method based on a thin layer chromatography, and belongs to the technical field of drug detection. The thin layer chromatography identification method comprises the following steps: taking astragaloside as a control product to identify astragalus membranaceus, taking chrysophanol and emodin as control products to identify largeleaf rhubarb, taking hirudo medicinal materials as a control product to identify hirudo, taking glycyrrhizin and licorice medicinal materials as control products to identify licorice, taking catalpol as a control product to identify radix rehmanniae, taking salvianolic acid B and salvia miltiorrhiza medicinal materials as control products to identify salvia miltiorrhiza, and taking naringin and neohesperidin as control products to identify aurantii fructus. The application establishes the thin layer chromatography identification method of the heart-protecting granule, adopts a special developing agent and a color developing mode, realizes accurate identification according to the spot color, fluorescence characteristics and other characteristics of the corresponding positions of the test product chromatogram, the control product and the control medicinal material chromatogram, the method is simple in operation, low in detection cost, strong in repeatability and specificity, and can effectively control the quality of the heart-protecting granule production process.

The present invention relates to a method for preparing catalpol 6-caffeate from the flowers of Spathodea campanulata, and its product and application. The method comprises the following steps: Step S1: Heating and refluxing the dried and pulverized flowers of Spathodea campanulata with a first solvent, filtering and then concentrating under reduced pressure to obtain a first extract; Step S2: Using a second solvent as an extractant to extract the first extract, the extraction times being 2-3 times, combining the supernatant and filtering and concentrating to obtain a second extract; Step S3: Using a resin as an adsorbent, eluting with water, a third solvent, and a fourth solvent respectively, collecting the fraction eluted with the fourth solvent, and concentrating to obtain a refined product; Step S4: Adding a fifth solvent to the refined product and precipitating at a low temperature, filtering and drying under reduced pressure after precipitation to obtain catalpol 6-caffeate. The catalpol 6-caffeate prepared by the present invention has the effects of inhibiting tyrosinase activity and repairing the skin barrier.

The invention relates to a method for determining the content of effective components in a Kunxinning preparation. Comprising the following steps: step 1, preparing a reference substance solution, namely respectively taking a proper amount of one or two or more of catalpol, rehmannia glutinosa liboside D, paeoniflorin, calycosin-7-glucoside, (-)-syringinol-4-O-beta-D-furanselinosyl-(1-2)-beta-D-glucopyranoside, curculigoside, icariin and baohuoside I reference substances; preparing a reference substance solution; step 2, preparation of a test solution: taking a Kunxinning preparation, weighing the Kunxinning preparation, placing the Kunxinning preparation in a measuring flask, adding methanol to dilute the Kunxinning preparation to a scale, filtering the Kunxinning preparation, and taking a subsequent filtrate; step 3, a determination method: respectively and precisely sucking the reference substance solution and the test solution, injecting the solutions into a high performance liquid chromatograph, and determining component types and / or component contents; the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler, water is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is performed, the flow velocity is 0.5-1.5 mL / min, the column temperature is 25-35 DEG C, the detection wavelength is 200-300 nm, and the sample size is 10-30 [mu] L.

The invention discloses an alveolar macrophage inhibitory natural product monomer and application thereof, and relates to the technical field of biological medicines. Seven natural product monomers including polyphyllin B, gentisic acid, catalpol, abscisic acid, aescin, ginsenoside and guaiacol are obtained through screening, and the menin / SETD2 interaction can be specifically inhibited. Experiments show that the monomers can inhibit expression of various inflammatory factors such as IL-1alpha, IL-1beta, IL-6 and the like induced by LPS in a dose-dependent manner; animal experiments prove that the monomers can obviously inhibit release of inflammatory factors induced by pathogens such as LPS and streptococcus pneumoniae, and can effectively relieve acute pneumonia and lung injury of mice. The provided natural product monomer is clear in target spot, specific in effect and broad in anti-inflammatory effect spectrum, and has important application prospects in treatment of cell / inflammatory factor storm related diseases caused by abnormal activation of alveolar macrophages.

This invention discloses a medicinal and edible herbal beverage for fundamental health restoration and its preparation process, belonging to the field of traditional Chinese medicine health beverage technology. The beverage is based on medicinal and edible ingredients such as ginger, poria cocos, platycodon grandiflorus, jujube seed, rehmannia glutinosa, ganoderma lucidum, polygonatum sibiricum, lily bulb, and jujube. It precisely combines active ingredients such as gingerol, hesperidin, platycodon saponin, jujube seed glycoside, catalpol, and total polysaccharides, achieving a balanced cooling and warming effect, and providing both nourishment and cleansing benefits. It can nourish yin and strengthen essence, calm the mind and invigorate the spleen, resolve stagnation and eliminate dampness, improving sub-health issues such as yin deficiency and essence depletion, spleen and stomach stagnation, and poor sleep. This invention employs a combined process of ultra-micro pulverization, compound enzymatic hydrolysis, anaerobic-microaerobic bidirectional microbial fermentation, low-temperature ultrasound-assisted extraction, and graded membrane purification and concentration, effectively improving the dissolution rate of active ingredients and the content of small molecule active substances. This solves the problems of low dissolution rate of active ingredients, easy inactivation of heat-sensitive substances, poor bioavailability, and difficulty in achieving efficacy in traditional processes.

The invention belongs to the technical field of medicines, and particularly relates to a composition for preventing and treating children obesity and a preparation method thereof. The composition comprises the following raw materials in parts by weight: 35-45 parts of active pharmaceutical ingredients and 8-15 parts of auxiliary materials, the active pharmaceutical ingredient is composed of sipunculus nudus polypeptide and catalpa glycoside in a mass ratio of 1: (1.5-3). The composition can inhibit the weight gain of mice with high fat diet, reduce the total cholesterol, triglyceride and low-density lipoprotein level in serum of the mice, and improve the high-density lipoprotein level in the serum. In addition, the composition can also improve liver fat accumulation and relieve fatty liver lesion caused by liver fat accumulation, and has no influence on renal functions.

A Catalpa bungei antibacterial composition, the Catalpa bungei antibacterial composition comprising a mixture formed by catalpol and 4,9-dihydroxy-α-lapachone. The mass fraction of catalpol is 50-65%, and the mass fraction of 4,9-dihydroxy-α-lapachone is 20-30%. The steps for separation and extraction using Catalpa bungei as a raw material are as follows: The powder of the heartwood part of Catalpa bungei is placed in dichloromethane solvent for cold soaking at room temperature, and the obtained extract is evaporated to dryness under reduced pressure to obtain a dark brown extract; the obtained dark brown extract is separated by silica gel column chromatography, eluted with a mixed solvent of dichloromethane and methanol, and then eluted with pure methanol after the elution is completed. The methanol eluate is collected and concentrated under reduced pressure to obtain the Catalpa bungei composition. The present invention not only has a decontamination function on human skin, but also has a good antibacterial effect, and can also enhance the market competitiveness.

The invention discloses application of catalpol in promoting macrophage IFN-gamma secretion and an application determination method of catalpol, and solves the technical problem of lack of research on effective components based on IFN-gamma tumor immunotherapy, autoimmune disease regulation, vaccine research and development, health care products, immunopotentiators and the like in the prior art. The application comprises application of catalpol in promotion of macrophage IFN-gamma secretion, and a determination method of the application comprises the following steps: step A, preliminary discovery and verification of effects, UUO mouse kidney differential gene analysis and kidney tissue IFN-gamma detection under different catalpol administration conditions; and B, verifying the effect again, namely detecting IFN-gamma mRNA in RAW264.7 cells under different administration conditions of catalpol and / or detecting IFN-gamma mRNA in mouse bone marrow macrophages before and after catalpol administration. The invention discloses the application of catalpol in promoting macrophage IFN-gamma secretion, and the catalpol has wide application prospects in tumor immunotherapy, autoimmune disease immune function regulation, anti-fibrosis, vaccine adjuvant research and development, health care products, immunopotentiators and the like.

According to the present invention, the high performance liquid chromatography is adopted, the five symbolic components in the fermented soybean fresh rehmannia root simultaneously-processed product are subjected to simultaneous quantitative analysis under the acetonitrile 0.1% phosphoric acid water system gradient elution condition, the method is simple, the catalpol, the rehmannia glutinosa libosch D, the leonuride, the daidzein and the genistein can be rapidly and simultaneously separated, and the peak pattern and the separation degree are good. The simultaneous determination method established by the invention is good in repeatability and accurate and reliable in result, can be used for determining the contents of catalpol, rehmannia glutinosa libosch D, leonurus glycoside, daidzein and genistein, can be used for research on a processing technology of a fermented soybean fresh-rehmannia root simultaneous processing process product, and can also provide a basis for quality control of the fermented soybean fresh-rehmannia root simultaneous processing product.

The invention discloses a liquid chromatography-mass spectrometry detection method of bulbus fritillariae cirrhosae lung-heat-clearing syrup, which comprises the following steps: respectively taking a reference substance and the bulbus fritillariae cirrhosae lung-heat-clearing syrup, adding methanol for dissolving to obtain a reference substance solution and a test solution, and injecting the reference substance solution and the test solution into an HPLC-QQQ / MS liquid chromatography-mass spectrometer for detection. The detection method disclosed by the invention can be used for detecting rehmannia glutinosa-D, catalpol, amygdalin, platycodin-D, ophiopogonin-C and ophiopogonin-D in the fritillaria cirrhosa lung-heat-clearing syrup, is used for identifying active ingredients in the fritillaria cirrhosa lung-heat-clearing syrup, and perfects the quality control standard of the fritillaria cirrhosa lung-heat-clearing syrup. According to methodology verification, the detection method is high in accuracy and sensitivity, can realize specific detection of the characteristic components of the bulbus fritillariae cirrhosae lung-heat-clearing syrup, and has practical application value.

The application discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses and belongs to the technical field of pesticide antiviral agents.Taking a tobacco mosaic virus coat protein as an example, small molecules including sugar rings, such as Swertiamarin I (A0) and ZINC85593196 (B0), are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. Based on this, further optimization is carried out, and six compounds similar to the structure of A0, such as 4-methylumbelliferone-beta-D-xyloside (A1), gardenoside (A2), 4-methylumbelliferone-beta-D-pyranoside (A3), 4-methylumbelliferone-beta-D-pyranoglucoside (A4), catalpol (A5) and esculin (hemihydrate) (A6), are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active ingredients screened by the above method have good inhibitory effect on plant viruses.

The invention discloses a traditional Chinese medicine composition for treating a neuronal intracellular inclusion body disease and a preparation method thereof, and belongs to the field of traditional Chinese medicines. The traditional Chinese medicine composition provided by the invention is prepared from 11 medicinal materials including turtle shell, concha haliotidis, ruddle, nacre mother of pearl, semen cassiae, gastrodia elata, chrysanthemum morifolium ramat, prepared rehmannia root, Chinese yam, stiff silkworm and scutellaria baicalensis. The method adopts a step-by-step extraction and refining process and comprises the following steps: firstly, decocting mineral water with water; combining medicine residues with animal-derived traditional Chinese medicines, and carrying out ethanol reflux extraction; and finally decocting with the rest of plant-derived traditional Chinese medicines. After all extracting solutions are combined, active ingredients are enriched through macroporous resin gradient elution, finally, dry paste powder is obtained through concentration and spray drying, and capsules are prepared. The yield of the finished product dry paste is 22.7%, and the content of active ingredients needs to meet the quality control standard that baicalin is larger than or equal to 9.2 mg / g, gastrodin is larger than or equal to 3.5 mg / g, and catalpol is larger than or equal to 4.8 mg / g.

The present application relates to the low-toxicity and high-efficiency composition for preventing and treating depression, which can effectively solve the problem of preparing the low-toxicity and high-efficiency medicine for preventing and treating depression, and the composition is composed of rehmannia extract and fluoxetine, wherein the rehmannia extract is one of rehmannia water extract, rehmannia alcohol extract, rehmannia glycoside D and catalpol. The rehmannia water extract, rehmannia alcohol extract, catalpol and rehmannia glycoside D in rehmannia are selected for the first time, and are matched with fluoxetine to form the composition, so as to provide the safe and high-efficiency anti-depression composition, greatly improve the drug advantage, partially solve the hidden danger of liver toxicity of the current anti-depression drug to the clinical safe drug use, help to improve the compliance of the depression patients, maintain the body health of the depression patients, promote the recovery of the patients, improve the life quality of the patients, and have significant economic and social benefits.

The invention discloses a flavor substance directional regulation and control enzymolysis method for a rehmannia and Chinese yam fermented beverage, which comprises the following steps: S1, gelatinizing rehmannia and Chinese yam at high temperature; s2, cooling the gelatinized slurry, putting the gelatinized slurry into an enzymolysis bin of fermentation equipment, adjusting the gelatinized slurry to be acidic, and adding beta-glucosidase for enzymolysis; s3, conveying the enzymolysis slurry to a fermentation bin of fermentation equipment, and then adding lactic acid bacteria for anaerobic fermentation; s4, after the acidity of the fermentation liquor is further reduced, saccharomycetes are added, and double-bacterium anaerobic fermentation is carried out at low temperature; and S5, after fermentation is completed, performing pasteurization, filtration and aging, and then performing filling. The beta-glucosidase is used for performing directional enzymolysis on iridoid glycoside flavor substances such as catalpol and the like, the problem that the fermented beverage has bitter taste is solved, double-bacterium combined aeration is subsequently used for fermentation, breeding of infectious microbes is inhibited, and the taste of the beverage is enriched by generated various lipid substances.