6 results about "Harpagoside" patented technology

The application discloses a crocus rhizosphere microorganism and application thereof, and belongs to the technical field of traditional Chinese medicinal material quality and quality improvement. The rhizosphere microorganism is obtained by using traditional bacterial separation and purification technology from a crocus rhodora plant crocus living body, belongs to the actinobacteria phylum, mycobacterium order, mycobacterium family and mycolicibacterium genus bacteria, is classified and named as (Mycolicibacterium frederiksbergense) SRB560, and the strain preservation number is CGMCC No. 29735. The application also discloses the application of the rhizosphere microorganism. The rhizosphere microorganism can effectively promote the growth and effective component content accumulation of radix scrophulariae, improves the medicinal material yield by 50.08% (P<0.05), and improves the total amount of harpagide and harpagoside by 25.56% (P<0.05). The rhizosphere microorganism has obvious effects on promoting the growth of radix scrophulariae and improving the quality of radix scrophulariae, and brings wide application prospects for the development of the radix scrophulariae industry.

This invention relates to the field of molecular biology technology of medicinal plants, and particularly to a protein, gene, and their application related to the biosynthesis of harpagoside in Scrophularia ningpoensis. The proteins disclosed in this invention related to the biosynthesis of harpagoside in Scrophularia ningpoensis include the Sni_B_54755.0 protein shown in SEQ ID NO.1; the Sni_B_54755.1 protein shown in SEQ ID NO.3; or the Sni_B_54755.9 protein shown in SEQ ID NO.5. In vitro and in vivo enzyme activity verification, UPLC and LC-MS product identification of these proteins confirmed that the above three proteins are related to the biosynthesis of harpagoside in Scrophularia ningpoensis. This provides a basis for the analysis of key enzymes in the biosynthesis of harpagoside in Scrophularia ningpoensis, the discovery of related functional genes, and metabolic engineering research.

The invention relates to a method for extracting a magic grass extract, in particular to a method for extracting harpagoside from magic grass, and aims to overcome the defect of long process period in the existing technology for extracting harpagoside. The method comprises the steps of raw material crushing and enzymolysis, organic solvent impurity removal, extraction, graded sedimentation, membrane separation, membrane concentration, extraction, crystallization and the like, the whole preparation process is mild in condition, the loss of the harpagoside under a high heat condition is avoided, the influence of temperature on the instability of the harpagoside is effectively avoided, and the method is suitable for industrial production. The activity and the purity of the harpagoside can be maintained, and the harpagoside with relatively high quality can be obtained.

The invention discloses a UHPLC-MS / MS (Ultra High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry)-based multi-component content determination method for a compound radix scutellariae throat-clearing capsule, which is characterized in that a multi-component simultaneous quantitative analysis method is established by adopting a UHPLC-MS / MS technology, and the quality of the compound radix scutellariae throat-clearing capsule is evaluated from the quantitative aspect. 21 components including p-hydroxybenzaldehyde, vanillin, caffeic acid, syringaldehyde, citric acid, tanshinol, chrysin, baicalein, genistein, quercetin, chlorogenic acid, neochlorogenic acid, rosmarinic acid, harpagide, puerarin, apigenin-7-O-beta-D-glucoside, baicalin, oleanolic acid, scutellarin, glycyrrhetinic acid and harpagoside are selected as quantitative indexes; and determining the content of the compound radix scutellariae throat-clearing capsule. According to the method, the contents of 21 components in the compound radix scutellariae throat-clearing capsule can be accurately measured at the same time within 14 min, and the method has good precision and stability.

This invention relates to the field of analytical detection technology, and particularly to the application of harpagoside or harpagoside in the identification of Scrophularia ningpoensis honey. This invention is the first to discover that harpagoside and harpagoside can serve as characteristic marker compounds for the identification of Scrophularia ningpoensis honey. Furthermore, this invention provides a method for identifying Scrophularia ningpoensis honey, which exhibits high specificity and sensitivity, with a detection limit of 5–20 μg / kg for the target compound, an accuracy between 87–108%, and a coefficient of variation of less than 15%. The identification method of this invention is simple to operate, highly efficient, and easy to promote and apply. It is of great significance for the identification and authenticity evaluation of Scrophularia ningpoensis honey and has broad application prospects.

The invention relates to a preparation method of harpagoside, and belongs to the technical field of natural product extraction. The method comprises the following steps: extracting uncaria gramineus with an extraction solvent to obtain a crude extract; the crude extract is sequentially subjected to extraction with an extraction solution, column separation and crystallization, and harpagoside is obtained, the extraction liquid is n-butyl alcohol. By optimizing the extraction conditions and the extraction method, the extraction efficiency and the purity of the harpagoside are remarkably improved. In addition, a special filler adopted in the column separation step can effectively improve the separation effect of a target compound, so that the finally obtained harpagoside has higher purity and better stability. The method not only is simple and convenient to operate, but also has good repeatability, and is suitable for the requirements of industrial production.