45 results about "Harpagoside" patented technology

The invention is suitable for the field of medicines and provides a drug composition for preventing and treating related diseases of ischemic stroke. The drug composition comprises the following components in parts: 1.0-3.5 parts of notoginsenoside R1, 4.0-7.5 parts of ginsenoside Rg1, 3.5-7.5 parts of ginsenoside Rb1, 1.0-3.0 parts of ginsenoside Re, 0.03-0.15 part of astragaloside, 0.02-0.010 part of calycosin-7-O-beta-D-glucopyranoside, 0.01-0.10 part of harpagoside, 0.002-0.010 part of cinnamic acid, 0.45-1.5 parts of salvianolic acid B, 0.02-0.10 part of cryptotanshinone and 0.02-0.10 part of tanshinone IIA. The drug composition provided by the invention has the functions of promoting blood circulation and removing blood stasis, supplementing qi and nourishing yin, can be used for preventing and treating the related diseases of the ischemic stroke and is capable of overcoming the shortcomings of component complexity and the like of traditional compound traditional Chinese medicine preparations.

The invention relates to the field of natural medicaments, in particular to a medical application of Harpagoside compound, i.e. the pharmacological effect of Harpagoside compound can be applied to treating metabolic diseases caused by insulin resistance or glycometabolism disorder complication caused by insulin resistance.

The invention discloses a freeze-drying method of figwort root. After the fresh figwort root are selected to be sliced, freeze-drying is conducted, factors such as the temperature, the rate of temperature change, and the duration are controlled during pre-freezing, step-by-step freezing, and heating drying so as to prevent effective ingredients of figwort root harpagide and harpagoside from beingdestructed during processing, and quality of a figwort root medical material is ensured. (That is, the figwort root index component content stipulated in the 2015 edition of Chinese pharmacopoeia), compared with a general drying method, the figwort root medical material produced by the freeze-drying method is stable and controllable, and is unaffected by external factors, and the quality of a finished medical material product is high.

The present invention is directed to compositions incorporating purified herbal ingredients for the treatment of conditions such as pain, inflammation, arthritic conditions and other chronic rheumatic diseases, muscle spasms, and headache, as well as methods for their use. The compositions incorporating purified herbal ingredients can incorporate the following combinations of ingredients: (1) harpagoside and paeoniflorin; (2) harpagoside, paeoniflorin, and bioperin; (3) harpagoside and bioperin; and (4) paeoniflorin and bioperin.

The invention discloses a detection method for an HPLC characteristic atlas of a Liang Di decoction. The method is characterized by comprising the following steps that (1) a tested object solution isprepared by taking a preparation of the Liang Di decoction to be detected; (2) a contrast solution is prepared by taking paeoniflorin and harpagoside; and (3) a contrast characteristic atlas is made by absorbing the tested object solution and the contrast solution and injecting the solutions into a high efficiency liquid chromatograph, generating the contrast characteristic atlas according to atlas obtained by measuring N batches of tested objects, and calculating relative retention time with other shared peaks by taking retention time of a chromatographic peak of the reference object as reference. The method is higher in precision, satiability and repeatability, and can be used to comprehensively and effectively control the quality of the Liang Di decoction.

The invention discloses a method for extracting harpagoside. The method includes the following steps: fresh figwort roots as a raw material are ground and then added with methanol, ethanol or acetone aqueous solution for reflux extraction, extract is added into a polyamide column for separation after the reagent is recovered, 5 to 10 BV water, 20 to 30 percent of 4 to 6 BV ethanol (methanol) solution and 50 to 70 percent of 5 to 7 BV ethanol (methanol) solution are sequentially used for elution, eluent is concentrated and then extracted with ethyl acetate for 4 to 6 times, extract is concentrated and added with petroleum ether for crystallization, methanol-chloroform mixture is then added for recrystallization, and after low-temperature drying, the harpagoside is obtained. The repeatability of the process is high, and the method is suitable for the production of high-content harpagoside.

The invention provides a preparation method of Xuanmai Ganjie granules. The method comprises the following steps: decocting and concentrating radix scrophulariae, radix ophiopogonis, liquorice and platycodon grandiflorus; and performing refluxing treatment on the concentrate for 2.5-3.5 hours while keeping the refluxing temperature at 55-65 DEG C to finally obtain granules. In the finished product of Xuanmai Ganjie granules prepared in the invention, the contents of active ingredients, namely harpagoside and glycyrrhizic acid, are remarkably increased, and the treatment effect of the medicine is correspondingly enhanced, which is a great beneficial technical progress; and on the premise of neither increasing production equipment nor changing production flow, low cost and high yield are realized, and tremendous economic and social benefits are created by the most economical way.

The invention discloses a method for preparing harpagoside and sibirioside A by using high-speed counter-current chromatography through separation and purification. The method comprises the steps of crushing a radix scrophulariae medicinal material, extracting by ethanol, and enriching by using macro-porous adsorption resin to obtain a crude extract; performing high-speed counter-current chromatography separation on the crude extract; forming a high-speed counter-current solvent system by using a solvent A, a solvent B and a solvent C of which the volume ratio is (1-5): (5-10): (1-10), wherein the solvent A is methanol, ethanol, n-butyl alcohol or isobutanol; the solvent B is ethyl acetate, propyl acetate or n-butyl acetate; the solvent C is water; an upper phase is a stationary phase, and a lower phase is a mobile phase, so that harpagoside and sibirioside A are prepared respectively. The method disclosed by the invention is used for preparing harpagoside and sibirioside A by using the high-speed counter-current chromatography, does not have irreversible adsorption, avoids the loss of samples, and has the characteristics of good separation effect, small solvent dose, no pollution, high efficiency and high speed.

The invention belongs to the field of processing of Chinese medicines, and particularly discloses a method for refining a figwort decoction piece and a product thereof. The method comprises the following steps of: preparing fresh figwort, removing impurities, drying or airing figwort till the mass percentage of water in figwort is 25-35 percent, piling for 40-50 days, and turning once every 3-6 days; and wetting figwort, softening, slicing and drying till the mass percentage of water is 2-15 percent to obtain the figwort decoction piece. The obtained figwort decoction piece has high medicinal ratio, is sliced in a standardized way, is not softened with a cooking process, is prepared with a simple process, and is convenient for industrial production; and the losses of harpagoside and harpagide in medicinal materials are small, so that the medicament effect is ensured.

A figwort root method is provided. Through effective control of a series of parameters, such as water adding amount, infiltration time and drying temperature, cinnamic acid, harpagide, harpagoside andother effective components in the processed figwort root are high in content. Compared with unprocessed figwort root, peak areas of the second and the third peaks in ten common characteristic peaks in fingerprint are reduced, peak areas of other peaks are all increased, and areas of the fifth peak, an S (cinnamic acid) peak, the seventh peak, the eighth peak and the tenth peak are obviously increased. Complex chemical components in a traditional Chinese medicine are compound bases of multiple effects of the traditional Chinese medicine. Through specific chromatograms before and after figwortroot processing, a plurality of components are changed during figwort root processing. The method is simple and suitable for large-scale production.

The invention provides a liquid increasing granule and a preparation method thereof. The preparation method comprises the following steps: taking 270g of radix scrophulariae, 216g of radix rehmanniae recens and 216g of radix ophiopogonis, adding 10 times of water, soaking for 0.5h, decocting for 1 hour, filtering, adding 8 times of water into medicine residues, decocting for 1 hour, filtering out decoction and the like. By adopting special granule drying equipment, the content of harpagoside in the prepared liquid increasing granule is high, and the liquid increasing granule can be applied to preparation of drugs for inhibiting Chinese hamster ovary (CHO) cell proliferation.

The invention relates to a preparation method of extract of granules containing radix scrophulariae, radix ophiopogonis, radix glycyrrhizae and radix platycodonis. The preparation method is characterized by comprising the following processing steps: first, soaking radix scrophulariae for 2-3 h, mixing the soaked radix scrophulariae with radix ophiopogonis, radix glycyrrhizae and radix platycodonis, adding 9-10 folds of water, carrying out soaking for 25-30 min, carrying out heating till the boiling state is achieved, carrying out first-time extracting, carrying out filtering, adding 9-10 foldsof water into the obtained filter residue, carrying out heating till the boiling state is achieved, carrying out second-time extracting, carrying out second-time filtering, adding 7-8 folds of waterinto the obtained filter residue, carrying out heating till the boiling state is achieved, carrying out third-time extracting, mixing the filtrates obtained after the three times of filtering, carrying out concentrating, carrying out standing, carrying out centrifugalizing, carrying out re-concentrating, and collecting the extract. According to the preparation method provided by the invention, themethod of shortening the soaking time is adopted for treating the traditional Chinese medicinal materials of the granules containing radix scrophulariae, radix ophiopogonis, radix glycyrrhizae and radix platycodonis, so that the content of harpagoside in the extract of the granules containing radix scrophulariae, radix ophiopogonis, radix glycyrrhizae and radix platycodonis is improved, and meanwhile, the time consumed by the process is shortened correspondingly.

The invention discloses a radix scrophulariae iridoid reference extract and a preparation method and application thereof. The preparation method comprises the following steps: (1) loading a scrophularia ningpoensis crude extract on a first silica gel column and eluting to obtain an eluted first silica gel column; (2) equally dividing the eluted first silica gel column into N sections along the direction from the column head to the bottom; obtaining a first target section and a second target section; respectively treating to obtain a scrophularia ningpoensis iridoid reference extract I and a scrophularia ningpoensis iridoid reference extract intermediate; (3) treating the scrophularia ningpoensis iridoid reference extract intermediate to obtain a scrophularia ningpoensis iridoid reference extract II; and (4) mixing the scrophularia ningpoensis iridoid reference extract I and the scrophularia ningpoensis iridoid reference extract II to obtain the scrophularia ningpoensis iridoid reference extract. In the radix scrophulariae iridoid reference extract obtained by the invention, the total content of harpagoside, anagloside C and harpagoside is higher than 60wt%.

The invention relates to a radix scrophulariae producing area processing and preparation integrated preparation process and a detection method. The method comprises the following steps: washing freshradix scrophulariae with water to remove impurities such as soil on the surface of the medicinal material and carrying out drying to reduce weight, sweating, slicing and then performing drying to obtain a product. Measurement is performed by using harpagide, harpagoside, polysaccharide, a water-soluble extract and an alcohol-soluble extract as investigation indexes. Single-factor investigation iscarried out on the weight reduction temperature, the weight reduction degree, the sweating temperature, the sweating time, the slicing shape, the slicing specification and the drying temperature in the radix scrophulariae producing area processing and preparation integrated process; the optimal radix scrophulariae producing area processing and preparation integrated process is optimized by adopting an orthogonal test and a comprehensive scoring method and the radix scrophulariae integrated preparation process and the detection method are explored. The process is stable, reasonable and feasible, and higher value is provided for guiding the production of radix scrophulariae medicinal materials.

The invention relates to a construction method of a radix scrophulariae medicinal material evaluation model and an evaluation method of a radix scrophulariae medicinal material quality. The construction method comprises the following steps: measuring the contents of harpagide, harpagoside and cinnamic acid in a radix scrophulariae medicinal material; acquiring physical characteristic parameters of the radix scrophulariae medicinal material; establishing a regression equation I of the physical characteristic parameter I of the radix scrophulariae medicinal material and the sum of the contents of harpagide and harpagoside; and establishing a regression equation II of the physical characteristic parameter II of the radix scrophulariae medicinal material and the content of the cinnamic acid. The physical characteristic parameters of the radix scrophulariae medicinal material and the contents of various index active components in the radix scrophulariae medicinal material are combined for evaluation, so that the quality of the radix scrophulariae medicinal material can be evaluated more objectively, accurately, efficiently and comprehensively.

The present disclosure relates to a dietary vegan supplement for treating joint pain in human comprising of natural ingredients as its constituents. The composition for the dietary vegan supplement for treating joint pain in human comprises of natural vitamin E (d-alpha-tocopherol), turmeric root extract (standardized to 95% curcuminoids), a plant material (devils claw) providing a rich source of harpagosides, calcium carbonate powder, green tea extract comprising standardized 90% polyphenols, 80% catechins, ginger, olive extract (olive oil powder), glucosamine HCL, flaxseed or flaxseed extracts thereof, and black pepper extract standardized to contain 95% piperine. In addition, the compositions provided herein can contain other ingredients such as vitamin D3 (cholecalciferol).

The invention relates to a detection method of a traditional Chinese medicine composition for nourishing yin and clearing away lung-heat. The method comprises the following steps: preparation of a reference substance solution: taking paeoniflorin, harpagoside and paeonol as reference substances, and dissolving with a solvent; preparing a test solution, namely extracting a to-be-detected sample byusing an extraction solvent, filtering an extracting solution, and taking subsequent filtrate as the test solution; injecting the reference substance solution and the test solution into a liquid chromatograph for determination, wherein a mobile phase A of the liquid chromatograph is water, a 0.005 mol/L-0. 04mol/L sodium dihydrogen phosphate aqueous solution or a 0.005 mol/L-0. 04mol/L potassium dihydrogen phosphate aqueous solution, and a mobile phase B of the liquid chromatograph is acetonitrile. The method provided by the invention realizes simultaneous determination of multiple componentsin the yin-nourishing lung-heat-clearing traditional Chinese medicine composition, has strong specificity and good separation effect, has accuracy, reproducibility and stability meeting scientific research and production requirements, and can effectively ensure the stability and controllability of product quality.

The invention discloses a plant skin-whitening composition, which is prepared from the following effective components: harpagoside, a xanthium sibiricum extract and matrix, wherein the weight ratio of the xanthium sibiricum extract to the harpagoside is equal to (1 to 1) to (30 to 1), and the matrix is prepared from vitamin E magnesium phosphate ester, polyglyceryl-3 diisostearate, glycolic acid, linseed oil and hyaluronic acid. The plant skin-whitening composition is prepared by taking the xanthium sibiricum extract as a main raw material and adding the harpagoside and the matrix, thus being low in price; the xanthium sibiricum extract is mixed with a small amount of the harpagoside, so that a good synergic effect for skin-whitening is achieved.

The invention discloses a reflux extraction method of delactation inhibition particles, and belongs to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: S1, weighing 20 parts of selfheal, 120 parts of raw malt, 10 parts of thunberg fritillary bulb, 5 parts of radix scrophulariae, 3 parts of edible tulip, 5 parts of kelp and 10 parts of oyster, S2, heating at 70 DEG C by adopting 6 times of 40% ethanol, and carrying out reflux extraction for three times for 0.5 h each time, S3, filtering, and concentrating the filtrate at 70 DEG C to recover the solvent, and S4, performing vacuum drying on the concentrated liquid medicine under the condition of 0.1 MPa to obtain dry extract, thereby obtaining the traditional Chinese medicine composition. The content of rosmarinic acid and the content of harpagoside in the prepared delactation inhibition and increase granules are 40.35 mu g/g and 8.03 mu g/g respectively. The preparation method is convenient to use, the obtained finished product is convenient to carry, take and store, meanwhile, the quality and efficacy of the medicine can be accurately controlled, recovery of a patient is facilitated, and practicability is achieved.