The invention relates to a preparation method of camellia oleosa seed oil with high
oxidation resistance, and belongs to the technical field of camellia oleosa seed oil. The preparation method comprises the following steps: mixing camellia seed
powder with
distilled water, carrying out ultrasonic treatment, preheating, adding immobilized compound
enzyme, mixing, stirring at a constant temperature, and
drying to obtain an intermediate; pressing the intermediate in a double-
screw press; the preparation method of the immobilized compound
enzyme comprises the following steps: preparing composite particles from
chitosan,
amino acid and
cellulose, adding an auxiliary agent and an aromatic
aldehyde solution to prepare a porous carrier, and immobilizing the compound
enzyme by the porous carrier. According to the method, after the camellia seeds are subjected to ultrasonic treatment, compound enzyme can permeate into a
plant tissue structure more easily, the extraction rate of
grease is increased, in addition,
chitosan,
cellulose and
amino acid are compounded to form a composite carrier, and the composite carrier and
syringaldehyde form a
Schiff base structure, so that the
heat stability and pH tolerance of the
immobilized enzyme are improved; the activity of the compound enzyme is improved and extraction of camellia oleosa seed oil is promoted.