Method for efficiently degrading tetracyclic and sulfonamide antibiotics by laccase

A technology of antibiotics and tetracyclics, applied in the field of enzyme engineering and technology and fermentation, can solve the problems of high price, unsatisfactory degradation effect, and difficulty in obtaining intellectual property rights, etc., and achieve the effect of broad application prospects
CN110577936AInactive Publication Date: 2019-12-17JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-12-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for efficiently degrading tetracyclic and sulfonamide antibiotics by laccase. The method comprises the following steps: S1, fermenting by adopting Pycnoporus sp. SYBC-L10 to produce laccase; and S2, firstly adjusting the pH value of a waste liquid containing tetracyclic antibiotics or sulfonamide antibiotics to 3.0-6.0, then adding the laccase obtained in the stepS1, simultaneously adding a small molecular mediator ABTS or syringaldehyde, standing and carrying out incubation for a period of time. The invention provides laccase capable of efficiently degradingtetracyclic and sulfanilamide antibiotics in the environment and a method. Compared with reported similar laccase, the laccase of the invention has the advantages that the effect is obviously quicker(generally, 50 mg L<-1> of antibiotics are degraded only within 5-10 min). Compared with a traditional physical-chemical method, the method of the invention is green, safe and environmentally friendly, meets the sustainable development requirement and has wide application prospects in the application of antibiotics to the future environment treatment.
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Description

technical field

[0001] The present invention relates to the fields of enzyme engineering and technology and fermentation technology, in particular to a method for efficiently degrading tetracyclic and sulfonamide antibiotics by fermenting a strain of Mycoporeus to produce laccase Background technique

[0002] Antibiotics left in the environment are considered to be a new class of pollutants, which pose a great risk to human health and the stability of the ecosystem. In recent years, how to remove antibiotics left in the environment has attracted widespread attention. Antibiotics are widely used in medicine, aquaculture and other industries, and are currently one of the most widely used drugs in the world. In recent years, laccases have played an important role in the degradation of antibiotics. However, the reaction time of the existing research needs several hours or even more than a few days, and the domestic laccases used for antibiotic degradation are mainly imported c...

Claims

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