Method for degrading antibiotics in aquaculture sewage by combining industrialized laccase and syringaldehyde mediator

An aquaculture and antibiotic technology, applied in the field of biochemistry, to achieve the effects of reducing consumption cost, good degradation effect and simple treatment process

Pending Publication Date: 2019-09-17
江西省水利科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Novozym 51003 laccase is widely used in printing and dyeing, papermaking and other industries, but its application in antibiotic sewage treatment is rarely reported

Method used

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  • Method for degrading antibiotics in aquaculture sewage by combining industrialized laccase and syringaldehyde mediator
  • Method for degrading antibiotics in aquaculture sewage by combining industrialized laccase and syringaldehyde mediator
  • Method for degrading antibiotics in aquaculture sewage by combining industrialized laccase and syringaldehyde mediator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: antibiotic degradation system 100ml, add aquaculture sewage 96,95,94ml successively, 1g / L antibiotic mother liquor respectively adds 1ml, 10mM syringaldehyde mediator mother liquor adds 1 (0.1mM), 2 (0.2mM), 3ml respectively (0.3mM), 2ml of Novozym51003 laccase was added, and 3 parallel samples were set up in each group. The pH of each system was adjusted to 6 respectively, and placed in a temperature-controlled shaking incubator at a temperature of 25° C. and a rotation speed of 150 rpm. At 180 minutes, take 1ml samples into 5ml centrifuge tubes, then add 1ml methanol and mix well to inactivate laccase to stop the reaction. Finally, after filtering through a 0.22 μm syringe filter, it was transferred to a 2ml brown sample bottle for determination of the remaining antibiotic concentration. No syringaldehyde mediator was used as the control group. Antibiotic degradation effects are as follows:

[0035]

Embodiment 2

[0036]Example 2: 100ml of antibiotic degradation system, add 97ml of aquaculture sewage, add 1ml of 1g / L antibiotic mother solution, add 1.87ml (0.187mM) of 10mM syringaldehyde mediator mother solution, add 0.051ml of Novozym 51003 laccase, and set 3 in each group Parallel sample. Adjust the pH of the reaction system to 5.5, and put it into a temperature-controlled shaking incubator at a temperature of 30.3° C. and a rotation speed of 150 rpm. At 180 minutes, take 1ml samples into 5ml centrifuge tubes, then add 1ml methanol and mix well to inactivate laccase to stop the reaction. Finally, after filtering through a 0.22 μm syringe filter, it was transferred to a 2ml brown sample bottle for determination of the remaining antibiotic concentration. No syringaldehyde mediator was used as the control group. Antibiotic degradation effects are as follows:

[0037]

Embodiment 3

[0038] Example 3: 100ml of antibiotic degradation system, add 96.9ml of aquaculture sewage, add 1ml of 1g / L antibiotic mother solution, add 2ml (0.2mM) of 10mM syringaldehyde mediator mother solution, add 0.088ml of Novozym 51003 laccase, and set 3 in each group Parallel sample. Adjust the pH of the reaction system to 5.3, and put it into a temperature-controlled shaking incubator with a temperature of 31° C. and a rotation speed of 150 rpm. At 180 minutes, take 1ml samples into 5ml centrifuge tubes, then add 1ml methanol and mix well to inactivate laccase to stop the reaction. Finally, after filtering through a 0.22 μm syringe filter, it was transferred to a 2ml brown sample bottle for determination of the remaining antibiotic concentration. No syringaldehyde mediator was used as the control group. Antibiotic degradation effects are as follows:

[0039]

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Abstract

The invention discloses a method for degrading antibiotics in aquaculture sewage by combining industrialized laccase and a syringaldehyde mediator, wherein sulfanilamide-based antibiotics in aquaculture wastewater are degraded by combining industrialized Novozym 51003 laccase and a syringaldehyde mediator. The method comprises: adding a certain amount of a sulfanilamide-based antibiotic mother liquor to aquaculture pond sewage, adding a syringin mediator mother liquor, finally adding Novozym 51003 laccase, adjusting to a suitable pH value, carrying out an oscillation reaction for 0.5-6 h at a certain temperature, and carrying out a catalytic oxidation degradation reaction so as to effectively degrade the sulfanilamide-based antibiotics in the sewage. According to the present invention, the industrialized laccase is used as the laccase source, and the ecological effect and the degradation effect of the mediator are comprehensively considered by determining the concentration of the syringaldehyde mediator, such that the treatment process is simple, the cost is low, and the pollutant removal rate is high.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for jointly degrading antibiotics in aquaculture sewage by using industrialized laccase Novozym 51003 laccase and syringaldehyde as reaction mediators. Background technique [0002] In recent years, reports on the detection of antibiotics in water environment have attracted more and more attention. In 2015, the publication of China's first antibiotic discharge and pollution map has attracted great attention from all walks of life. The ecological effects caused by environmental antibiotic residues have been continuously concerned by researchers at home and abroad. In order to reduce the potential ecological risk of antibiotic residues to the environment, especially in the context of the implementation of sewage permits in my country in recent years, research on the degradation and removal of environmental trace pollutants such as antibiotics has become a hot spot ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/342C02F2101/305
Inventor 丁惠君张本浩刘聚涛郭春晶钟家有胡芳代涛涛楼倩张维昊
Owner 江西省水利科学研究院
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