Bacillus coagulans YSF17 and application thereof

A technology of Bacillus coagulans, CCTCCM2021965, applied in the field of microorganisms, can solve the problems affecting the effective survival of Bacillus coagulans, achieve the effect of reducing content, increasing content, and large energy consumption

Active Publication Date: 2021-11-05
四川润格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the problem of effective survival of Bacillus coagulans in the actual complex environment in the field of food and feed has not been solved. Bile salts can greatly affect the effective survival of Bacillus coagulans

Method used

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  • Bacillus coagulans YSF17 and application thereof
  • Bacillus coagulans YSF17 and application thereof
  • Bacillus coagulans YSF17 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Screening and separation and purification of condensation

[0041] Collect the health farm sheep in Baoding City, Hebei Province. Fresh animal feces were collected in sterile cotton swabs and tested in sterile centrifugal tubes, each animal sample was taken. 10 g of the collection of fresh feces samples was called, diluted with 90 ml of sterile water and shaked at room temperature for 30 min. The sterile gauze was prepared to filter the filtrate of the above shock-treated manure sample, and then filtered three times. The resulting filtrate was placed in a 80 ° C water bath in a hot treatment for 15 min to remove non-spore. After the above heat treatment, the feces filtration gradient was diluted with a suitable gradient, coated with each of the YPD and MRS solid plates containing bromromol mexhydrol, and cultured in a 50 ° C incubator for 24 h. The colonies surrounded by YPD and MRS solid plates have a yellow discoloration ring are static in YPD and MRS liquid medium, re...

Embodiment 2

[0068] Bacillus Coagulan YSF17 was identified by 16S RDNA sequence as a Bacillus genus. The strain has been stored in the China Typical Culture Safety Center, and the number of Materials, CCTCC M 2021965, and the deposit site is China Wuhan University, the preservation time is August 2, 2021.

[0069] The strain culture method of Condensed Bacillus YSF17 is as follows:

[0070]YPD plate purification separation: Anti-Condensed cytoplatte YSF17 under aseptic conditions, overprinting in YPD plate, placed in a 50 ° C incubator inverted culture 24 h, picking a single colony into liquid YPD to carry out Next activation After activation, the experiment was carried out.

[0071] YPD medium composition (g / L): yeast powder 10, protein 胨 20, glucose 20, water 1000; 115 ° C sterilization for 20 min. A 1.5% agar powder was added additional solid medium.

[0072] Method of detection of the number of bacteria: Accurately weigh 10g sample to add 250 ml of triangular bottles with 90 ml of steril...

Embodiment 3

[0080] Example 3 Using Condensed Bacillus YSF17 Fermented Cotton Seed Meal Degradation of Cottonol

[0081] The cottonseed meal was added to the 0.6% fermentation medium and 0.6% of the fermentation medium, which was used as a basic solid fermentation medium, and the YPD solid medium activated coagulum pylori YSF17, challenged the single bacterius to the YPD liquid medium, and stand at 50 ° C The culture solution after 24 h is a seed liquid having a solid-state fermentation, and the induction amount is 15% and the liquid ratio 1: 1 seed liquid and water into solid fermentation medium, mix mix mix, adjust the initial pH 6, fermentation temperature is 50 At ° C, the fermentation time is 48 h to medium solid state fermentation, and the resulting fermentation of cottonseed meal is dried, and it is sealed with a sealed bag, placed in the drying conditions.

[0082] In the above embodiment, the cotton flt in the cottonseed meal was reduced from 1173 mg / kg to 111.3 mg / kg, and the det...

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Abstract

The invention discloses bacillus coagulans YSF17 and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in China Center for Type Culture Collection, the preservation number is CCTCC M 2021965, the preservation place is Wuhan University, Wuhan, China, and the preservation time is August 2, 2021. The bacillus coagulans YSF17 disclosed by the invention has the capacity of secreting amylase and cellulase, can produce acid by utilizing 11 monosaccharides or oligosaccharides, and can resist the high temperature, bacteria can grow at 60 DEG C, and the survival rate of spores can reach 80% after the spores are treated at the high temperature of 90 DEG C for 30 minutes; the strain can resist various phenolic substances such as vanillin, syringaldehyde, benzaldehyde, gossypol and the like, is used for fermentation of cottonseed meal and soybean meal, can effectively reduce the content of anti-nutritional factors such as gossypol, stachyose, raffinose and the like, shortens the fermentation period, implements cost reduction and synergism, and has very good application prospects in development and application of protein feeds.

Description

Technical field [0001] The present invention relates to the field of microbial, particularly to a Bacillus coagulans YSF17 their use. Background technique [0002] Lactic acid bacteria are normal flora of human and animal advocates dominant bacteria, can produce large amounts of lactic acid fermentation of sugar, regulate advocate flora balance, enhance immunity and so on. Although lactic acid bacteria has the characteristics of traditional strong ability to produce lactic acid, but in the heat resistance, bile salts, gastric acid resistant capacity in this area is very poor, Bacillus coagulans spores is the only type of lactic acid bacteria found so far, with good resistance to inhibition of lactic acid produced by breeding enteric pathogens, and thus maintain intestinal health, so both Bacillus coagulans spores and lactic acid producing great features to make up for this shortcoming. Bacillus coagulans is applied as a feed additive in animal breeding is secreted by a variety of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/30A23K10/37A23K20/20A23K20/24C12R1/07
CPCA23K10/12A23K10/37A23K10/30A23K20/30A23K20/24Y02P60/87
Inventor 刘超杨丹露彭楠田建平梁运祥
Owner 四川润格生物科技有限公司
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