106 results about "Bacillus coagulans" patented technology

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention discloses Witzia ciliaris NCU-33 and application thereof, and belongs to the technical field of microbial medicines. The bacillus coagulans NCU-33 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus coagulans NCU-33 is CGMCC NO.31779. The strain is separated from a fresh excrement sample of healthy people; the strain is capable of ameliorating and / or preventing rheumatoid arthritis by anti-inflammatory and immunomodulatory effects.

This application relates to the field of feed processing technology and discloses a highly absorbable feed composition for nursery pigs containing compound probiotics. The composition includes the following ingredients: a basal diet, a live bacteria compound powder composed of freeze-dried *Lactobacillus plantarum* powder and freeze-dried *Bacillus coagulans* powder, medium-chain triglycerides, a PIT-responsive compound emulsifier composed of polysorbate-80 and sorbitan monooleate, anhydrous trehalose, glycerol, a micron-sized confinement carrier, which is a porous carrier obtained by high-temperature expansion and vacuum degassing of perlite powder, and fumaric acid. This invention employs anhydrous trehalose and glycerol to construct a high-permeability system, combined with medium-chain triglycerides and a compound emulsifier to form an oil-phase encapsulation, achieving the technical effect of effectively blocking external moisture penetration and improving the heat resistance of the bacteria.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

Aiming at a lactic acid return loss phenomenon existing in a process for producing lactic acid by microbial fermentation, the invention firstly provides application of a target gene in inhibiting lactic acid return loss in lactic acid production by a microbial fermentation method, and the target gene is a lactic acid permease gene lutP and / or an iron-sulfur oxidase gene cluster lutABC. On the basis of the application, the invention also provides a method for inhibiting lactic acid return consumption in lactic acid production by a microbial fermentation method, and a bacillus coagulans engineering strain with low lactic acid return consumption and high sugar-acid conversion rate. According to the method, effective inhibition of intracellular transport and / or metabolism of lactic acid is realized, so that the problem of lactic acid return consumption in a process for producing lactic acid through microbial fermentation is solved, and the technical effect is remarkable. According to the engineering strain, a LutR palindromic sequence in a lactic acid permease gene lutP promoter region is modified, and / or an iron-sulfur oxidase gene cluster lutABC is inactivated, so that transmembrane transport and / or intracellular metabolism of lactic acid are / is effectively inhibited, a lactic acid return loss phenomenon is remarkably weakened, the sugar-acid conversion efficiency is improved, the fermentation process flow is simplified, the production cost is reduced, and the engineering strain is suitable for industrial production. And the method has important value for large-scale production.

The invention belongs to the technical field of fish culture, and particularly relates to bacillus coagulans, a traditional Chinese medicine fermentation preparation and application of the bacillus coagulans and the traditional Chinese medicine fermentation preparation. The technical problem to be solved by the invention is to provide a new way for improving excessive heavy metals in fish culture. The technical scheme of the invention is as follows: the bacillus coagulans DZNBC-06-16 is provided, and the preservation number of the bacillus coagulans DZNBC-06-16 is CGMCC (China General Microbiological Culture Collection Center) NO.35929. The strain can be subjected to anaerobic fermentation by utilizing a culture medium containing traditional Chinese medicines (honeysuckle and scutellaria baicalensis). The fermentation product is rich in flavones, butyric acid and lactic acid, and also can resist oxidation. The fermentation liquor is added into the fish basal feed, so that the influence of excessive heavy metals (copper, zinc and chromium) in the feed on the growth and shape indexes of zebra fish is remarkably improved.

The invention relates to the field of bacteriostasis of clostridium difficile, in particular to bacillus coagulans, clostridium butyricum, a combination of the bacillus coagulans and the clostridium butyricum and application of the bacillus coagulans and the clostridium butyricum in relieving clostridium difficile infection. The bacillus coagulans is bacillus coagulans BC99 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 21801, and the bacillus coagulans BC99 is a bacillus coagulans BC99 of which the preservation number is CGMCC NO. The clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 33734, and the clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC NO. The invention discloses inhibition effects of the bacillus coagulans, the clostridium butyricum and the combination thereof on clostridium difficile, and regulation and improvement of intestinal infection caused by the clostridium difficile.

This invention relates to the fields of microbiology and biotechnology, and discloses Bacillus coagulans PPX-13 and its applications. Strain PPX-13 is a thermotolerant, multifunctional phosphate-solubilizing biocontrol bacterium that can reduce rhizosphere soil acidification in tea trees, improve fertility, enhance the microbial ecological environment, improve the functional microbial community and structure, inhibit mold communities and structure, and increase the yield of spring tea. It has been identified as Bacillus coagulans. This bacterium exhibits mesophilic and thermotolerant phosphate-solubilizing activity; phytase activity; multiple enzyme activities; and mesophilic antagonistic activity against isolates of anthracnose fungal pathogens. This strain holds promise for development into a novel agricultural microbial agent, which is of great significance for mitigating soil acidification, reducing fertilizer use, activating soil nutrients, improving soil quality, and increasing plant (especially tea) yield and quality.

The invention relates to the technical field of microbial fermentation, in particular to a complex microbial inoculant as well as a preparation method and application thereof in promoting compost fermentation. The composite bacterial agent provided by the invention comprises a composite bacterial liquid and a matrix, wherein the composite bacterial liquid comprises bacillus licheniformis and bacillus coagulans; the mass ratio of the bacillus licheniformis to the bacillus coagulans is 1: (0.4-2.5). When the complex microbial inoculant provided by the invention is used for fermenting compost, the fermentation starting time in winter can be remarkably shortened, the core temperature of the compost is increased by 10-50 DEG C, and the fermentation period is shortened from conventional 12-16 days to 5-6 days.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The application discloses a composite biological deodorant, which comprises the following components in parts by weight: 1-3 parts of Bacillus coagulans slurry; 1-3 parts of Saccharomyces cerevisiae slurry; 1-3 parts of Lactobacillus plantarum slurry; 10-30 parts of nutrient solution A; and 20-60 parts of nutrient solution B. The composite biological deodorant is prepared by using the composite system of the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the Lactobacillus plantarum slurry, and combining with a specific nutrient solution carrier. The Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance. Metabolic products secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Lactobacillus plantarum. Bacteriocins produced by the Lactobacillus plantarum selectively inhibit staphylococcus aureus, escherichia coli and other foul-smelling pathogenic bacteria, and degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide. The three components are synergistic, so that the deodorization and bacteriostasis effects are achieved. The product has no strong peculiar smell, and the risk of contamination by miscellaneous bacteria is effectively avoided.

Nutritional supplements and compositions for activating the GLP-1 peptide and / or other hormones in a subject in order to regulate blood sugar, weight, and hunger of the subject are described in this application. The compositions include a first nutritional supplement containing specific amounts of hydrolyzed Acacia gum, lemon bioflavonoids, chromium, Berberis aristate, and hesperidin. The compositions also include a second nutritional supplement containing specific amounts of flax seed oil, resistant potato starch, resistant tapioca fiber, kombucha, Bacillus coagulans, Bacillus clausii, and allulose. A third composition includes the first and second nutritional supplements containing these ingredients in sufficient amounts to regulate the GLP-1 peptide and / or other hormones. These compositions are able to increase the GLP-1 concentration in the blood by up to about 140% without binding directly to any GLP-1 receptors. Other embodiments are also described.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine decoction piece for improving immunity and a preparation method thereof. The preparation method of the traditional Chinese medicine decoction piece comprises the following steps: S1, weighing the following raw material medicines in parts by weight: 10-25 parts of rhizoma atractylodis macrocephalae, 7-12 parts of radix pseudostellariae and 2-12 parts of fructus lycii, cleaning, drying, and sequentially performing steam treatment and crushing treatment to obtain traditional Chinese medicine powder slurry; s2, adding bacillus sorola desert into the traditional Chinese medicine powder slurry, uniformly stirring, fermenting, and sterilizing to obtain a first fermented product; s3, adding bacillus coagulans into the first fermentation product, uniformly stirring, fermenting, and sterilizing to obtain a second fermentation product; s4, the second fermentation product is subjected to vacuum drying, smashing and tabletting, and the traditional Chinese medicine decoction pieces for improving the immunity are obtained. The traditional Chinese medicine decoction piece has a good effect of enhancing immunity.

The invention discloses a bacillus subtilis CR-6 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the bacillus subtilis CR-6 is CGMCC (China General Microbiological Culture Collection Center) No.35148. The bacillus coagulans ER-6 has the characteristic of high yield of chitinase, has a strong contact killing effect on meloidogyne incognita and reniform reniform nematodes no matter fermentation liquor or fermentation supernate, is applied to prevention and control of agricultural pests, does not involve broad-spectrum chemical toxins, does not have harmful residues, and meets the requirements of green agriculture and ecological safety. Meanwhile, the bacillus coagulans ER-6 disclosed by the invention also has a growth promoting effect on plant growth, and the yield of crops is remarkably increased.

A method of treating, preventing or ameliorating at least one symptom of obsessive compulsive disorder (OCD) in a subject is disclosed. The method comprises a step in which a multistrain probiotic formulation is administered to the subject. The multistrain probiotic formulation includes the bacterial strains Bacillus coagulans, Lactobacillus rhamnosus, Bifidobacterium lactis, Lactobacillus plantarum, Bifidobacterium breve and Bifidobacterium infantis and Glutamine in a concentration range of 100-500 mg.

The present application provides a composite probiotic for relieving constipation, and a use thereof. The composite probiotic for relieving constipation is composed of a Bifidobacterium animalis subsp. lactis Bla36 strain with a preservation number of CGMCC No. 24029, a Lactobacillus rhamnosus LRa05 strain with a preservation number of CGMCC No. 24377, a Bacillus coagulans BC99 strain with a preservation number of CGMCC No. 19487, and a Bifidobacterium bifidum Bbi32 strain with a preservation number of CGMCC No. 16923. The four strains have a synergistic effect in relieving constipation symptoms.

The present application relates to the field of agricultural biotechnology and plant disease control technology, and specifically discloses a composition for preventing and controlling rape root rot, a preparation method and application thereof. The composition is prepared by fermentation of the following components in parts by weight: tea seed cake 10-30 parts, oyster shell powder 20-30 parts, waste molasses 10-20 parts, brewer's yeast powder 1-5 parts, xylo-oligosaccharide industrial residue 1-3 parts, and a composite fermentation agent containing lactobacillus plantarum, bacillus coagulans and propionic acid-producing propionibacterium 1-3 parts. The fermentation process adopts a two-stage pH control timing inoculation process: in the first stage, brewer's yeast is inoculated; in the second stage, the composite fermentation agent is inoculated under acidic conditions, and through synergistic metabolism, the sugar source is efficiently converted into a short-chain fatty acid composite system mainly composed of lactic acid and propionic acid. The prevention and control effect on rape root rot is significantly improved, and the composition also has the functions of improving soil and promoting crop growth, and has the advantages of high efficiency, environmental protection and multi-effect combination.

The invention relates to the technical field of biological medicine manufacturing, discloses an ancient herbal enzyme oral liquid for treating digestive system diseases and a preparation method, and aims to solve the problems that in the prior art, adaptability of strains and herbal fibers is insufficient, directional enzyme production induction is deficient, probiotic activity retention is poor, and a flora, fiber and enzyme cooperative closed loop cannot be constructed. The preparation method comprises the following steps: by taking genuine hemp seeds, Chinese herbaceous peony and the like as raw materials, matching with rhizosphere-separated saccharomyces cerevisiae Y-01, lactobacillus acidophilus L-03 and bacillus coagulans C-05, carrying out three-step directional fermentation of substrate pretreatment, flora colonization and enzymatic synthesis to produce CAZymes and SCFA, embedding probiotics through trehalose-chitosan, compounding a stabilizer adaptive preparation, and adjusting the ratio of the raw materials according to an IBS subtype. The fiber degradation efficiency and the functional product synthesis efficiency are improved, the gastric acid tolerance and the storage stability of the probiotics are enhanced, different IBS subtype requirements are met, and an effective means is provided for treatment of digestive system diseases.

The invention relates to the technical field of fermented feeds, in particular to a pennisetum sinese bacterium enzyme synergistic fermented feed as well as a preparation method and application of the pennisetum sinese bacterium enzyme synergistic fermented feed. The invention discloses a pennisetum sinese bacterium enzyme synergistic fermentation method. The method comprises the following steps: S1, crushing pennisetum sinese sections, and sieving to obtain micronized pennisetum sinese; s2, mixing saccharomyces cerevisiae, bacillus coagulans, lactobacillus casei, cellulase and water to obtain a zymophyte aqueous solution; s3, mixing the zymophyte aqueous solution with the micronized pennisetum sinese, and performing solid-state fermentation to obtain the pennisetum sinese bacterium enzyme synergistic fermentation feed. According to the application, the saccharomyces cerevisiae, the bacillus coagulans, the lactobacillus casei and the cellulase are used for synergistically fermenting the pennisetum sinese, so that the dry matter degradation rate and the crude protein content of the pennisetum sinese are increased, the contents of crude fibers and tannin are reduced, and the growth performance and the immunity of pigs and poultry are improved.

The invention relates to the technical field of fermented drinks, in particular to antioxidant orange soda water and a preparation method thereof. According to the orange soda water, orange juice is used as a main raw material, a multi-strain stepped fermentation process is adopted, bacillus coagulans, saccharomyces boulardii and lactobacillus plantarum are sequentially inoculated, and matrix degradation, functional precursor release, aroma framework and oxygen-expelling environment creation, flavor roundness and biological preservation are achieved respectively. A fermentation product is compounded with functional ingredients such as vitamin C, sodium D-isoascorbate, a citrus essential oil nano-emulsion and microencapsulated citrus polyphenol, and then high-pressure treatment terminal sterilization and high-power carbon dioxide carbonation filling are performed. The process effectively improves the oxidation resistance, flavor complexity and storage stability of the orange soda water, and is suitable for development of functional carbonated beverages.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus coagulans 18 and application thereof in fermentation production of L-lactic acid. According to the invention, heavy ion beam mutagenesis is combined with high-temperature adaptive evolution breeding to obtain the high-temperature-resistant bacillus coagulans, specifically bacillus coagulans 18, and the preservation number of the bacillus coagulans 18 is CGMCC (China General Microbiological Culture Collection Center) No. 33049. The high-temperature resistance of the strain is remarkably improved, efficient production of lactic acid can be achieved at high temperature, particularly, a two-stage heating method process control strategy developed for the strain can remarkably improve the raw material conversion rate of the strain, and the strain is suitable for industrial production of lactic acid, reduces the economic cost of lactic acid biological manufacturing and is suitable for application and popularization.

The application discloses a feed composition and a preparation method and application thereof, and relates to the technical field of feed additives.The feed composition comprises the following components in parts by weight: 4-6 parts of protease, 1-3 parts of phytase, 5-8 parts of bacillus subtilis, 5-8 parts of bacillus coagulans, 40-60 parts of oil, and 2-4 parts of emulsifier.The specific bacteria and enzymes are used for synergistic effect in the feed composition, so that the prepared feed can not only eliminate the muddy smell of farmed fish, but also reduce the abdominal fat rate of grass carp and improve the tightness of fish meat.

The invention discloses an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, and relates to the technical field of biological medicines and microecological preparations, in particular to an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, the active ingredients comprise bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, and bacillus capable of interfering the drug-resistant enterobacter quorum sensing system. The bacillus subtilis can be bacillus coagulans or bacillus subtilis; saccharomycetes capable of adsorbing the drug-resistant enterobacter and consuming necessary trace elements thereof, such as saccharomyces cerevisiae with optimized cell walls; the oligosaccharide mixture can be utilized by beneficial bacteria and can generate inhibitory products after being subjected to specific enzymolysis by drug-resistant enterobacter, and galactooligosaccharide, xylooligosaccharide and seaweed-derived oligosaccharide are mixed according to a specific proportion. The composition can be used for reducing the loading capacity of drug-resistant enterobacter in intestinal tracts of mammals, restoring the balance of intestinal flora and reducing the systemic infection risk. The preparation method comprises the step of mixing the active ingredients with a pharmaceutically or food acceptable carrier to prepare an oral dosage form.

The invention relates to the field of microbial fermentation application, in particular to a solid-state fermentation type light cheese flavor cream base material and a preparation method thereof.The preparation method of the solid-state fermentation type light cheese flavor cream base material comprises the following steps that S1, strain activation is conducted, specifically, an activation culture medium is inoculated with bacillus coagulans for culture activation; s2, secondary activation of strains; S3, preparation of a solid-state fermentation base material; s4, inoculating; s5, performing solid-state fermentation; s6, sterilization and enzyme deactivation treatment; and S7, performing high-speed shearing or spray drying on the solid-state fermentation base material subjected to sterilization and enzyme deactivation treatment to obtain the light cheese flavor cream base material. The prepared cream base material can provide rich light cheese flavor, is used in foods in various forms, endows the foods with light cheese fragrance and taste, meets the ever-increasing flavor requirements of people, can reduce cheese and processed cheese components required to be added into the foods, and reduces the product cost.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".