991 results about "Bacillus coagulans" patented technology

The present invention discloses compositions and methodologies for the utilization of probiotic organisms in therapeutic compositions. More specifically, the present invention relates to the utilization of one or more species or strains of lactic acid-producing bacteria, preferably strains of Bacillus coagulans, for the control of gastrointestinal tract pathogens, including antibiotic-resistant gastrointestinal tract pathogens, and their associated diseases by both a reduction in the rate of colonization and the severity of the deleterious physiological effects of the colonization of the antibiotic-resistant pathogen. In addition, the present invention relates to the utilization of therapeutic compounds comprised of lactic acid—producing bacteria and anti-microbial agents such as antibiotics, anti-fungal compounds, anti-yeast compounds, or anti-viral compounds. The present invention also discloses methodologies for: (i) the selective breeding and isolation of probiotic, lactic acid-producing bacterial strains which possess resistance or markedly decreased sensitivity to anti-microbial agents (e.g., antibiotics, anti-fungal agents, anti-yeast agents, and anti-viral agents); and (ii) treating or preventing bacteria-mediated infections of the gastrointestinal tract by use of the aforementioned probiotic bacterial strains with or without the concomitant administration of antibiotics. While the primary focus is on the treatment of gastrointestinal tract infections, the therapeutic compositions of the present invention may also be administered to buccal, vaginal, optic, and like physiological locations.

Compositions including a non-pathogenic lactic acid-producing bacteria, such as a Bacillus species, spores or an extracellular product of B. coagulans, formulated for oral administration to the intestinal tract for inhibiting bacterial gastrointestinal infections are described. Methods and systems using the compositions for treating gastrointestinal infections, particularly sudden infant death syndrome (SIDS) are also disclosed.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

The invention discloses a micro-ecological fruit-vegetable enzyme preparation method. The method comprises the steps of culturing 4 to 6 of six probiotics including lactobacillus acidophilus, bacillus coagulans, bacillus natto, saccharomyces cerevisiae, enterococcus faecium and bifidobacterium in a culture medium comprising ingredients such as brown sugar, liquorice and fresh sweet potato, and then culturing the probiotics through a diluted culture liquid containing brown sugar and liquorice in an activating manner to prepare a micro-ecological preparation, wherein the prepared micro-ecological preparation is a fermenting agent which is used for fermenting fruit pulp of fresh fruit and vegetable and traditional Chinese herbs. The microorganism enzyme in the ecological preparation and the natural vegetable enzyme in fruits, vegetables and Chinese herbs are compounded into a composite enzyme supplement by utilizing a micro-ecological fermentation technology, i.e. the micro-ecological fruit-vegetable enzyme. The micro-ecological fruit-vegetable enzyme can be made into a liquid type or a solid type (powder). An ideal effect is achieved in the fields such as medicine, healthcare and beauty.

Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans, suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.

The invention discloses a production method of L-lactic acid and the special condensate bacillus CASH CGMCC No. 2184. L-lactic acid can be gotten by cultivating the special condensate bacillus CASH CGMCC No. 2184. Per litter of fermentation culture medium of the condensate bacillus comprises at least one of the following five nitrogen sources: yeast powder 5 to 15g, peptone 5 to 15g, soybean peptide 5 to 15g, soybean peptone 5 to 15g, and cottonseed protein 10 to 20g; in addition, the fermentation culture medium comprises glucose 80 to 170g (the content of initial glucose), calcium carbonate 50 to 100g and water; the pH of the fermentation culture medium is 5.5 to 7. With glucose as the substrate, under the condition of 45 to 60DEG C and with 98 to 99% of sugar acid conversion, the condensate bacillus CASH CGMCC No. 2184 efficiently ferments to produce L-lactic acid with optical purity of 99%; the concentration of the L-lactic acid maximum can attain 190 g/l and the volume production efficiency maximum can attain 5.7 g/l/h.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu/g; lactobacillus acidophilus is not less than 1.0*10cfu/g; lactobacillus plantarum is not less than 1.0-10cfu/g; bacillus natto is not less than 1.0*10cfu/g; aspergillus niger is not less than 1.0*10cfu/g; and candida utili is not less than 1.0*10cfu/g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention relates to an environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture. An environment-friendly composite microbial water purifying agent comprises the following components in percentage by weight: 20 to 40 percent of bacillus subtilis, 10 to 20 percent of bacillus licheniformis, 10 to 15 percent of bacillus megaterium, 10 to 15 percent of brevibacillus laterosporus, 5 to 10 percent of bacillus pumilus, 5 to 20 percent of bacillus coagulans and 2 percent of biological activating agent. The high-concentration composite microbial preparation is prepared by screening eight strains of probiotics by simulating the physiological functions of normal microbial flora in an aquaculture environment and through scientific compatibility, so that division and cooperation amount the bacterial strains can be realized, a biological enhancement technology can be combined with an indigenous flora activating technology, complex problems in a culture water body can be solved completely, the using amount is small, the cost is low, and the competitive advantage is remarkable.

The present invention discloses a preparation method and application of Bacillus coagulans and a spore preparation cultured in high density, and belongs to the microbe technical field. A Bacillus coagulans strain is classified and named Bacillus coagulans JSSW-LA-07 and is preserved as CGMCC No.2602 in China General Microbiological Culture Collection Center. The Bacillus coagulans strain JSSW-LA-07 can produce spore under an aerobic condition and produce a small amount of L-lactic acid in the metabolism during a course of fermentation. The Bacillus coagulans strain can produce L-lactic acid with a higher purity under an anaerobic condition. The strain does not produce substances which can be harmful to human and cultured animals, has the characteristics of innocuity, on residue, no side effect and the like, has a great significance for production of green and safe livestock, fowls and marine products and improvement of cultivation ecological environment, and can be widely applied in the cultivation production of high yield, high quality, high efficiency, synusiologic and safe livestock, fowls and marine products, and the regulation and control of the ecological environment of cultivation.

The invention relates to a condensate bacillus feed additive and a preparation method, belonging to the technical field of microbial feed additives. The additive uses one or several of the fish meal, soybean meal, cottonseed meal, urea, ammonium sulfate, diammonium hydrogen phosphate, soybean peptone and yeast extract as the nitrogen source, and also takes one or several of the glucose, lactose, starch, cane sugar, maize flour and bran as the carbon source for fermentation; the number of viable bacteria reaches more than 1 multiplying 109 CFU/ml; after the additive is further concentrated and dried or directly adsorbed to the carrier, bacillus powder is obtained through spray-drying, and the number of viable bacteria reaches more than 1 multiplying 1010 CFU/g. The novel bacillus product produced with the submerged liquid fermentation method provided in the invention is free from toxic side effects, drug resistance and residue; the product can endure processing, intestinal habitat and storage.

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention provides a compound biological strain for feed. The compound biological strain comprises enterococcus faecalis, lactobacilli, saccharomycetes, bacillus subtilis and bacillus coagulans, wherein the bacterial count ratio of the enterococcus faecalis to the lactobacilli to the saccharomycetes to the bacillus subtilis to the bacillus coagulans is (0.5 to 1.5):(0.5 to 1.5):(0.1 to 0.5):(4 to 6):(0.5 to 1.5). The compound biological strain for feed and a preparation method of fermented feed provided by the invention have the advantages that the release of crude protein and amino acid in coarse feed can be promoted, and the animal absorption is facilitated.

A method of treating autism using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.

The invention discloses novel medical application of Bacillus coagulans, and in particular relates to application of the Bacillus coagulans as an active ingredient to preparing preparations for preventing and controlling shit odor and shit odor poisoning syndrome caused by shit odor toxin generated by harmful bacteria.

The invention discloses an antibiotic complex aquatic feed additive which is prepared from the following components in percentage by weight: 1-2 percent of probiotics, 8-9 percent of prebiotics and 20-30 percent of carriers. The probiotics comprise natto bacillus, bacillus coagulans and clostridium freeze-dried powder, the prebiotics comprise xylo-oligosaccharide, ganoderma meat amylase and oligochitosan, and the carriers comprise montmorillonite and corn starch. The antibiotic complex aquatic feed additive not only has the advantages of stable thalli and long survival time, but also has the effectiveness of united multiple roles. the invention also provides a mixed feed containing the antibiotic complex aquatic feed additive, which can obviously improve the digestion function of animals, increase the growth performance of animals of fishes, shrimps, soft-shelled turtles, and the like, decrease the feed factor, enhance the immunity of the animals and obviously improve the meat quality of animals.

An animal-derived lipid is disclosed that is useful as a carrying agent for anti-microbial formulations. Pharmaceutical and other preparations including Emu Oil are also described as profoundly useful components in anti-bacterial, anti-fungal, and anti-viral treatments. This lipid material is extracted from the Emu (Dromais Novae-Hollandiae), an indigenous bird of Australia and New Zealand. The present invention also discloses therapeutic compositions comprising Emu Oil in combination with an extracellular product of Bacillus coagulans or Pseudomonas lindbergii strain, comprising a supernatant or filtrate of said culture suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. Additionally, the aforementioned therapeutic composition may also include an anti-microbial, anti-mycotic, and/or anti-viral agent. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions comprising Emu Oil in combination with an extracellular product of Bacillus coagulans or Pseudomonas lindbergii strain suitable for topical application to the skin or mucosal membranes of a mammal. Similarly, the aforementioned method may also employ a therapeutic composition additionally containing an anti-microbial, anti-mycotic, and/or anti-viral agent.

The invention provides a complex bacterium for bean pulp fermentation. The complex bacterium is composed of bacillus subtilis, bacillus coagulans, aspergillus niger, aspergillus oryzae, lactobacillus plantarum and saccharomyces cerevisiae. The complex bacterium provided by the invention is obtained through repeated scientific experiment and reasonable combination based on physiological metabolism feature of the microorganism, the complex bacterium is vigorous in growth metabolism, is capable of secreting a plurality of enzymes, acids and other metabolites, and is capable of efficiently decomposing macromolecule protein, anti-nutritional factors and sensitization factors in the bean pulp, obviously increasing content of soluble protein, small peptide, organic acid, protease and vitamin and the number of probiotics and antibacterial peptide, improving the digestion utilization rate, inhibiting the growth of pathogenic bacteria and reducing the use of antibiotic; and meanwhile, the complex bacterium has acid fragrance of fermentation and is good in food attractant effect. The bacteria strains forming the complex bacterium are included in a feed additive variety catalogue (2013) issued by the department of agriculture, the complex bacterium is safe and reliable, and can be directly used for the industrial production for the bean pulp fermentation.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses the syndrome of diarrhea with loose and fetid stools caused by harmful bacteria in intestine, especially relates to a kind of probiotics and prebiotics used for treating said syndrome and the compound product. Said probiotics comprises bacillus coagulans, butanoic acid clostridial vaccine, infant bifidobacteria, young bifidobacteria, long bifidobacteria, Bifidobacterium bifidum and bacillus subtilis, and etc. The prebiotics can be soybean oligosaccharide and other oligosaccharides. The probiotics and prebiotics and their compound can be taken orally, and they can remove indole, scatal, sulfur, amino and amine from intestinal canal.