208 results about "Bacillus coagulans" patented technology

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The present invention discloses a process for preparing lactic acid by mechanically enhanced ammonia method from straw-like biomass. The enzymatic hydrolysate of solid fibers obtained by the mechanically enhanced ammonia method from straw-like biomass is used as the carbon source of the fermentation medium in the process of fermenting lactic acid. The preparation of the carbon source in the fermentation medium comprises the following steps: S1: treating straw-like lignocellulosic biomass with a pretreatment liquid containing ammonia water and inorganic ammonium salts in a twin-screw device; S2: subjecting the obtained material to a heat preservation reaction, and after solid-liquid separation, obtaining solid fibers containing holocellulose; S3: enzymatically hydrolyzing the obtained solid fibers with Trichoderma reesei cellulase and xylanase, and performing solid-liquid separation, and the obtained supernatant is used as the carbon source in the fermentation medium for fermenting L-lactic acid. The present invention uses the sugar liquid obtained by enzymatic hydrolysis and saccharification of pretreated holocellulose, inoculates Bacillus coagulans to ferment L-lactic acid after supplementing nitrogen source and inorganic salts, and can greatly improve the concentration and conversion rate of L-lactic acid.

The invention relates to a probiotic preparation for improving intestinal health of pets and application of the probiotic preparation. The bacterial strains in the probiotic preparation consist of a bacillus coagulans BC66 bacterial strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 21879, a pediococcus acidilactici PA153 bacterial strain with the preservation number of CCTCC NO: M 2023230, a lactobacillus plantarum LP194 bacterial strain with the preservation number of CGMCC 21766, a bifidobacterium animalis BA20 bacterial strain with the preservation number of CGMCC No. 21830 and a lactobacillus reuteri LR108 bacterial strain with the preservation number of GDMCC No: 63095. According to the probiotic preparation, five specific probiotics are compounded, the five strains are matched and promoted with one another, and synergistic interaction is achieved on the effect of improving the intestinal health of pets, specifically, the digestive ability of the pets on nutrients is improved; the immunity of pets is improved and the diarrhea rate is reduced; the content of beneficial bacteria in intestinal tracts of pets is increased.

The invention provides a method for preparing a protease beverage by fermenting cyperus esculentus powder and a product thereof. Comprising the steps of directional pretreatment, fermentation medium preparation, segmented fermentation and optional blending, specifically, directional pretreatment adopts low-temperature extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the fiber structure and antinutritional factors of soybean meal are effectively broken; in the fermentation stage, lactobacillus plantarum, saccharomyces cerevisiae and bacillus coagulans composite flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the system is reduced to 4.0-4.5, and then L-cysteine hydrochloride-vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the protease activity is larger than or equal to 320 U / mL, the total number of viable bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the human gastrointestinal tract environment, and the method has wide industrialization prospects.

The invention discloses Witzia ciliaris NCU-33 and application thereof, and belongs to the technical field of microbial medicines. The bacillus coagulans NCU-33 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus coagulans NCU-33 is CGMCC NO.31779. The strain is separated from a fresh excrement sample of healthy people; the strain is capable of ameliorating and / or preventing rheumatoid arthritis by anti-inflammatory and immunomodulatory effects.

The invention provides a compound feed for promoting beef cattle growth and improving meat quality and a production method thereof, and belongs to the technical field of feed preparation. The compound feed comprises the following components in parts by weight: 100 parts of basal feed, 7-10 parts of a functional additive and 0.3-0.5 part of disodium inosinate, and the functional additive comprises a postbiotic compound, a fatty acid regulating agent, a bacterium enzyme synergist and trace elements, wherein the metagen complex comprises a lactobacillus plantarum metagen extract and a bacillus coagulans metagen extract, the fatty acid regulating agent comprises conjugated linoleic acid glyceride and medium-chain fatty acid, and the bacterial enzyme synergist comprises beta-mannase and bacillus subtilis. According to the compound feed, the growth performance and the meat quality can be synchronously improved, and a reliable processing technical guarantee is provided for improving the growth performance and the meat quality of beef cattle.

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.

The invention relates to the technical field of functional microbial preparations, in particular to a biological preparation for relieving intestinal discomfort caused by alcohol and a preparation method and application thereof.The main technical scheme is that the biological preparation is prepared from, by weight, 5-20 parts of bacillus coagulans, 10-30 parts of pediococcus pentosaceus, 5-10 parts of lactobacillus rhamnosus and 0-20 parts of saccharomycetes. 0-5 parts of bifidobacterium longum and 0-5 parts of lactococcus lactis. By constructing an alcohol metabolism-intestinal protection dual-function synergistic system, specific florae such as bacillus coagulans, pediococcus pentosaceus and lactobacillus rhamnosus are scientifically compatible with functional components such as inulin and L-cysteine, alcohol is efficiently metabolized, and a carbon source is generated; the technical problems of low metabolic efficiency, weak intestinal protection and serious gastric acid inactivation of a traditional product are comprehensively solved.

The invention discloses active probiotic soft sweets capable of improving intestinal health and enhancing immunity and a high-temperature preparation method of the active probiotic soft sweets. The method comprises the following steps: preparing soluble carbohydrate into an adapter solution with the water activity of 0.7-0.9, the pH value of 5.0-7.0 and the temperature of 40-50 DEG C; adding a certain amount of bacillus coagulans powder into the adaptive solution, and keeping the temperature at 40-50 DEG C for 20-50 minutes to prepare a pre-activation solution; preparing a sugar glue solution; auxiliary materials are added into the carbohydrate gum solution to prepare a blended carbohydrate gum solution, the pH value of the blended carbohydrate gum solution is 3.0-6.0, and the temperature is 60-80 DEG C; adding the pre-activated solution into the blended carbohydrate glue solution, and preserving heat for 10-40 minutes at the temperature of 60-80 DEG C to passivate; and pouring the passivated sugar glue solution into a soft sweet mold at 65-85 DEG C for shaping, and drying until the moisture is 16-18% to obtain the active probiotic soft sweet. The active probiotic soft sweets prepared by the preparation method disclosed by the invention are high in survival rate of probiotics, can keep stable in the delivery period, and have the activity of improving intestinal health and enhancing immunity.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

The invention provides a preparation method of a microbial viable bacterium concentrated laying hen feed, which comprises the following steps: culturing bacillus subtilis and bacillus coagulans, and then carrying out enlarged culture; cooling the fermentation liquor to 4-10 DEG C, and centrifuging to obtain bacterial sludge; soybean meal and corn protein powder are uniformly mixed, sieved and dried, mixed powder is obtained, the bacterial sludge and the mixed powder are mixed and stirred, and a bacterial sludge-carrier mixture is obtained; adding the bacterial sludge-carrier mixture into a chitosan acetic acid solution, adding a calcium chloride solution, stirring, sieving, and washing to prepare microspheres; adding the microspheres into a sodium alginate solution again, mixing, stirring and washing to obtain coated microspheres; and freeze-drying and crushing the coated microspheres. The problems of gastric acid tolerance, storage stability and processing applicability of the viable bacterium preparation are solved through an innovative process, and the viable bacterium preparation has wide market prospects and social value.

The invention relates to a complex microbial inoculant for fermenting and regenerating chicken manure and application of the complex microbial inoculant. The complex microbial inoculant consists of a first complex microbial inoculant and a second complex microbial inoculant which are independently packaged, bacterial strains in the first complex microbial inoculants consist of bacillus coagulans, bacillus subtilis, aspergillus niger and saccharomyces cerevisiae; the bacterial strain in the second complex microbial inoculant is composed of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus buchneri. According to the invention, seven probiotics are combined for use, the first complex microbial inoculant performs aerobic fermentation on a substrate containing chicken manure, unstable factors in chicken manure raw materials are reduced, and a high-quality raw material basis is provided for regenerated feed; wherein the second complex microbial inoculant performs anaerobic fermentation on a substrate containing chicken manure, so that the growth of harmful bacteria is inhibited, the safety of the regenerated product is further ensured, beneficial metabolites are generated, and the palatability of the regenerated product is improved. The finally obtained regenerated feed not only can reduce the feeding cost, but also can regulate the intestinal health, promote the growth of animals and reduce the morbidity.

This application relates to the field of feed processing technology and discloses a highly absorbable feed composition for nursery pigs containing compound probiotics. The composition includes the following ingredients: a basal diet, a live bacteria compound powder composed of freeze-dried *Lactobacillus plantarum* powder and freeze-dried *Bacillus coagulans* powder, medium-chain triglycerides, a PIT-responsive compound emulsifier composed of polysorbate-80 and sorbitan monooleate, anhydrous trehalose, glycerol, a micron-sized confinement carrier, which is a porous carrier obtained by high-temperature expansion and vacuum degassing of perlite powder, and fumaric acid. This invention employs anhydrous trehalose and glycerol to construct a high-permeability system, combined with medium-chain triglycerides and a compound emulsifier to form an oil-phase encapsulation, achieving the technical effect of effectively blocking external moisture penetration and improving the heat resistance of the bacteria.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

The invention relates to an anti-aging and whitening ligustroside-rich composition and a preparation method and application thereof.The preparation method of the composition comprises the following steps that 1, dried osmanthus fragrans flowers and flaxseeds are smashed and then sieved, obtained osmanthus fragrans flower powder and obtained flaxseed powder are mixed according to the mass ratio of 10: (1-3), and mixed powder is obtained; (2) adding deionized water into the mixed powder, and sterilizing to obtain a mixed material; (3) inoculating bifidobacterium adolescentis and saccharomyces cerevisiae into the mixed material for anaerobic fermentation, and sterilizing to obtain an anaerobic fermentation product; and (4) inoculating bacillus coagulans into the anaerobic fermentation product to carry out aerobic fermentation, sterilizing, filtering and drying to obtain the anti-aging and whitening composition rich in ligustrum glycoside. The composition provided by the invention has excellent whitening and anti-aging effects.

The present invention relates to a method for preparing probiotic microcapsules, particularly a method for preparing Bacillus coagulans microcapsules. The method employs a combination of inulin and xanthan gum to encapsulate Bacillus coagulans microcapsules. The microcapsules are activated and microencapsulated, and the inulin and xanthan gum are used to prepare the wall material. Sterile physiological saline is used as a solvent, to which Bacillus coagulans is added. The solution is homogenized to obtain a uniform solution, and then freeze-dried to obtain the microcapsules. The method improves the survival rate and stability of Bacillus coagulans. The prepared Bacillus coagulans microcapsules have high resistance and strong barrier properties, overcoming the limitations of Bacillus coagulans in consumption and storage.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

Aiming at a lactic acid return loss phenomenon existing in a process for producing lactic acid by microbial fermentation, the invention firstly provides application of a target gene in inhibiting lactic acid return loss in lactic acid production by a microbial fermentation method, and the target gene is a lactic acid permease gene lutP and / or an iron-sulfur oxidase gene cluster lutABC. On the basis of the application, the invention also provides a method for inhibiting lactic acid return consumption in lactic acid production by a microbial fermentation method, and a bacillus coagulans engineering strain with low lactic acid return consumption and high sugar-acid conversion rate. According to the method, effective inhibition of intracellular transport and / or metabolism of lactic acid is realized, so that the problem of lactic acid return consumption in a process for producing lactic acid through microbial fermentation is solved, and the technical effect is remarkable. According to the engineering strain, a LutR palindromic sequence in a lactic acid permease gene lutP promoter region is modified, and / or an iron-sulfur oxidase gene cluster lutABC is inactivated, so that transmembrane transport and / or intracellular metabolism of lactic acid are / is effectively inhibited, a lactic acid return loss phenomenon is remarkably weakened, the sugar-acid conversion efficiency is improved, the fermentation process flow is simplified, the production cost is reduced, and the engineering strain is suitable for industrial production. And the method has important value for large-scale production.

This invention discloses a method for processing bamboo shoot residue that synergistically enhances the content of soluble dietary fiber and its bound polyphenols. Using waste bamboo shoot residue as raw material, the fermentation bacteria are all food-grade. Specifically, a multi-strain coupled fermentation is employed, including *Bacillus belye*, *Bacillus natto*, *Trichoderma kangsei*, *Cladosporium*, *Aspergillus pamoateus*, and *Bacillus coagulans*. This significantly alters the ratio and content of soluble and insoluble dietary fiber in the bamboo shoot residue and substantially increases the content of dietary fiber-bound polyphenols, synergistically enhancing the content of soluble dietary fiber and its bound polyphenols, thereby producing high-quality dietary fiber. This invention is simple to operate, low in cost, and allows for easier control of fermentation conditions. It is highly efficient and does not easily damage the non-covalent bonds (hydrogen bonds, electrostatic or ionic bonds, etc.) or covalent bonds (ester bonds, ether bonds, etc.) binding dietary fiber and polyphenols. It also has no significant impact on the pH and temperature of the environment, and can significantly increase the content of soluble dietary fiber and its bound polyphenols in bamboo shoot residue.

The invention belongs to the technical field of fish culture, and particularly relates to bacillus coagulans, a traditional Chinese medicine fermentation preparation and application of the bacillus coagulans and the traditional Chinese medicine fermentation preparation. The technical problem to be solved by the invention is to provide a new way for improving excessive heavy metals in fish culture. The technical scheme of the invention is as follows: the bacillus coagulans DZNBC-06-16 is provided, and the preservation number of the bacillus coagulans DZNBC-06-16 is CGMCC (China General Microbiological Culture Collection Center) NO.35929. The strain can be subjected to anaerobic fermentation by utilizing a culture medium containing traditional Chinese medicines (honeysuckle and scutellaria baicalensis). The fermentation product is rich in flavones, butyric acid and lactic acid, and also can resist oxidation. The fermentation liquor is added into the fish basal feed, so that the influence of excessive heavy metals (copper, zinc and chromium) in the feed on the growth and shape indexes of zebra fish is remarkably improved.

The invention relates to the field of bacteriostasis of clostridium difficile, in particular to bacillus coagulans, clostridium butyricum, a combination of the bacillus coagulans and the clostridium butyricum and application of the bacillus coagulans and the clostridium butyricum in relieving clostridium difficile infection. The bacillus coagulans is bacillus coagulans BC99 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 21801, and the bacillus coagulans BC99 is a bacillus coagulans BC99 of which the preservation number is CGMCC NO. The clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 33734, and the clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC NO. The invention discloses inhibition effects of the bacillus coagulans, the clostridium butyricum and the combination thereof on clostridium difficile, and regulation and improvement of intestinal infection caused by the clostridium difficile.

This invention relates to the fields of microbiology and biotechnology, and discloses Bacillus coagulans PPX-13 and its applications. Strain PPX-13 is a thermotolerant, multifunctional phosphate-solubilizing biocontrol bacterium that can reduce rhizosphere soil acidification in tea trees, improve fertility, enhance the microbial ecological environment, improve the functional microbial community and structure, inhibit mold communities and structure, and increase the yield of spring tea. It has been identified as Bacillus coagulans. This bacterium exhibits mesophilic and thermotolerant phosphate-solubilizing activity; phytase activity; multiple enzyme activities; and mesophilic antagonistic activity against isolates of anthracnose fungal pathogens. This strain holds promise for development into a novel agricultural microbial agent, which is of great significance for mitigating soil acidification, reducing fertilizer use, activating soil nutrients, improving soil quality, and increasing plant (especially tea) yield and quality.

The present invention relates to the technical field of soil improvement, and discloses a carbon-based soil conditioner and a preparation method thereof. In the present invention, biochar, humic acid-based modified hydrogel, diatomite, hydroxyapatite, Bacillus licheniformis, and Bacillus coagulans are stirred and mixed, and then a polyvinyl alcohol solution is sprayed into the mixture, granulated and dried to obtain the carbon-based soil conditioner. Imidazoline quaternary ammonium salts and quaternary phosphonium salts can adsorb on the negatively charged cell surface of bacteria through their positive charges, and the hydrophobic groups are inserted into the lipid layer, causing the leakage of intracellular substances and leading to the death of bacteria; guanidine groups destroy the cell membrane of bacteria through strong positive charge adsorption, bind and coagulate nucleic acids and enzymes, playing an antibacterial role; the nitrogen atoms in the imidazole group and triazine group act as hydrogen bond acceptors, enhancing the hydrogen bond interaction with the hydrogel molecular chain and improving the water retention performance of the conditioner; the benzene ring hydrophobic groups form reversible cross-linking points through hydrophobic association, constituting a dynamic three-dimensional network and improving the water retention performance of the conditioner.

The invention relates to the technical field of microbial fermentation, in particular to a complex microbial inoculant as well as a preparation method and application thereof in promoting compost fermentation. The composite bacterial agent provided by the invention comprises a composite bacterial liquid and a matrix, wherein the composite bacterial liquid comprises bacillus licheniformis and bacillus coagulans; the mass ratio of the bacillus licheniformis to the bacillus coagulans is 1: (0.4-2.5). When the complex microbial inoculant provided by the invention is used for fermenting compost, the fermentation starting time in winter can be remarkably shortened, the core temperature of the compost is increased by 10-50 DEG C, and the fermentation period is shortened from conventional 12-16 days to 5-6 days.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The utility model relates to a fermentation tank convenient for expanding culture of bacillus coagulans in the technical field of fermentation equipment. Comprising a fermentation tank body, a stirring assembly is arranged in the fermentation tank body, and the stirring assembly comprises a stirring rod and stirring blades; a defoaming assembly is arranged on the stirring rod in the fermentation tank body; the plurality of stirring blades are fixed on the stirring rod below the defoaming assembly; the defoaming assembly comprises a defoaming pipe with the two ends plugged, a row of defoaming teeth are evenly arranged on the lower surface of the defoaming pipe, a liquid drainage hole is formed between every two adjacent defoaming teeth and communicated with the defoaming pipe, a liquid guide pipe is fixedly connected to the middle of the defoaming pipe, a liquid storage box is fixed to the top in the fermentation tank body, the stirring rod penetrates through the liquid storage box, and the stirring rod penetrates through the liquid storage box. A liquid inlet cavity is formed in the stirring rod, the liquid inlet cavity is communicated with the liquid storage tank, and the top of the liquid guide pipe extends into the liquid inlet cavity. The defoaming teeth on the defoaming pipe are matched with a defoaming agent discharged from the liquid discharge hole, so that an efficient defoaming effect is achieved, and production is facilitated.