395 results about "Bacillus coagulans" patented technology

A compound bacterial powder preparation, and a preparation method therefor and the use thereof. The compound bacterial powder preparation comprises Bifidobacterium animalis, Lactiplantibacillus plantarum and Bacillus coagulans, wherein the deposit number of the Bifidobacterium animalis strain is CGMCC No.25681. The compound bacterial powder preparation can stimulate macrophage RAW264.7 of a mouse to generate cell factors TNF-α and IL-6, thereby enhancing immunity; can significantly enhance the cellular immune function, the humoral immune function and the mononuclear-macrophage function of a mouse, and has the function of enhancing immunity; and can increase the ink propelling rate and has the functional potential of relaxing the bowels.

The invention particularly relates to a method for extracting ACE (Angiotensin Converting Enzyme) inhibitory peptide from tilapia skin. The tilapia skin is a by-product rich in collagen, and has important development significance as a screening raw material of the ACE inhibitor. The invention provides a method for extracting an ACE (Angiotensin Converting Enzyme) inhibitor by taking tilapia skin as a raw material, which comprises the following steps of: degreasing the tilapia skin, crushing, adding acid liquor, extracting to obtain fish skin collagen crude protein, adding enzyme into the fish skin collagen crude protein for enzymolysis, and reserving the part with the molecular weight of less than 3kDa in an enzymolysis product, namely the ACE inhibitory peptide. Particularly, the enzyme is a product of bacillus subtilis or bacillus coagulans under gelatin-induced fermentation.

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses a livestock composite biological feed prepared from probiotics and a method thereof, and relates to the technical field of livestock feeds. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus niger BNCC147472, enterococcus faecalis BNCC186075, yarrowia lipolytica BNCC193899, nutrient substances, mineral trace elements, vitamins, amino acids and prebiotics. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus The compound biological feed fermented by adopting various probiotics can improve the antioxidant level of the broiler chicken, improve the health condition of intestinal tracts, increase the villus height and the villus-implicit ratio of the jejunum of the broiler chicken and reduce the occurrence of intestinal inflammation, so that the growth performance of the broiler chicken is promoted, and the immune performance of the body is improved.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention relates to the technical field of screening and application of probiotics, and particularly provides a novel bacillus coagulans strain and application of the novel bacillus coagulans strain. The bacillus coagulans is screened from naturally fermented soybean paste, has good biological safety, strong stress resistance to gastrointestinal tract environment, high yield of exopolysaccharide and protease, strong bacteriostasis and oxidation resistance, can efficiently degrade cholesterol, is beneficial to maintaining intestinal health, and can be widely applied to the fields of food, health care products and the like.

The invention belongs to the field of microorganisms. The invention provides an application of a probiotic composition in preparation of medicines for preventing and treating ulcerative colitis, the probiotic composition comprises a combination of bacillus coagulans and clostridium butyricum, and the combination of bacillus coagulans and clostridium butyricum comprises a combination of bacillus coagulans and clostridium butyricum. The bacillus coagulans is bacillus coagulans JSSW-LA-07-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.10182, and the clostridium butyricum is clostridium butyricum JSIM-MCB20040312 with the preservation number of CGMCC No.1647. The bacillus coagulans and the clostridium butyricum are used for preparing the bacillus coagulans and the clostridium butyricum. The probiotic composition has the capacity of preventing and treating ulcerative colitis, the ulcerative colitis treating effect is better than that of positive control medicine salazosulfapyridine, the obvious ulcerative colitis treating effect is achieved, the inflammatory factor content and the oxidative stress level are remarkably reduced, inflammatory cell infiltration is reduced, intestinal flora imbalance is improved, and the effect of preventing and treating ulcerative colitis is achieved. Meanwhile, the effect of promoting planting of probiotics is achieved.

The invention discloses a composition for dispelling effects of alcohol and protecting liver and application thereof, and belongs to the technical field of biology. The composition comprises the following components in percentage by mass: 0.5-2% of probiotics, 6-20% of beta-glucan, 10-20% of corn peptide and 50-80% of medicinal and edible substances, and the probiotics are mixed bacterial powder prepared by freeze-drying bacillus coagulans BCN019 and lactobacillus salivarius JYLS-372 and then mixing the bacillus coagulans BCN019 and the lactobacillus salivarius JYLS-372 according to a mass ratio of (0.1-1): 1; the medicinal and edible substance is obtained by mixing a hovenia dulcis thunb extract, a kudzuvine root extract and a turmeric extract in a mass ratio of (0.1-1): (0.1-1): (0.1-1). The composition can relieve headache, dizziness and other uncomfortable symptoms caused by drinking; the contents of ethanol and acetaldehyde in the body can be obviously reduced, and the metabolism of the liver is relieved; meanwhile, by improving the intestinal microecology, gastrointestinal discomfort symptoms caused by excessive drinking can be effectively reduced, and hangover reaction is relieved.

The invention relates to an application of a compound microbial agent in promoting phosphorus and potassium solubilization of soil and increasing the content of available phosphorus and potassium in the soil. The bacterial strains in the compound microbial agent consist of a bacillus coagulans BC66 bacterial strain, a lactobacillus plantarum LP194 bacterial strain and a lactobacillus acidophilus LA71 bacterial strain. The invention also relates to an application of the compound microbial agent in promoting phosphorus and potassium solubilization of the soil and increasing the content of available phosphorus and potassium in the soil, and the bacterial strains in the compound microbial agent consist of a bacillus coagulans BC66 bacterial strain, a lactobacillus plantarum LP194 bacterial strain and a lactobacillus acidophilus LA71 bacterial strain. The invention develops a brand-new microbial preparation capable of promoting the phosphorus and potassium solubilizing effect of soil, and researches find that the three strains are mutually matched and have synergistic interaction on the effects of promoting phosphorus and potassium solubilizing of soil, improving the content of available phosphorus and potassium in soil, improving the utilization rate of phosphate fertilizer and potassium fertilizer and increasing the yield of fruit trees, and the yield of the fruit trees is increased under the condition that the using bacterium amount is consistent. Compared with a single strain intervention mode or other strain combined intervention mode, the microbial preparation has the advantage that the effects are obviously improved. Therefore, the compound microbial agent provides a new strategy for promoting phosphorus and potassium solubilization of soil, increasing the content of available phosphorus and potassium in soil, increasing the utilization rate of phosphate fertilizer and potassium fertilizer and increasing the yield of fruit trees.

The invention discloses a fermented Chinese herbal medicine preparation for improving eggshell quality and a preparation method of the fermented Chinese herbal medicine preparation, Chinese herbal medicines mainly comprise motherwort, folium artemisiae argyi, radix isatidis and jujube powder, and fermentation probiotics mainly comprise bacillus subtilis, bacillus coagulans, enterococcus faecalis, lactobacillus plantarum and saccharomycetes. The fermented traditional Chinese medicine preparation produced by the invention has the effects of improving intestinal health and immunity and improving oxidation resistance and stress resistance of laying hens in laying hen production, so that the egg quality and eggshell quality of the laying hens are improved.

The invention relates to the technical field of extraction and utilization of chemical substances in walnut green seedcases, in particular to a production process of urolithin A. The production process specifically comprises the following steps: obtaining the walnut green seedcases; walnut green seedcase is mashed into pulp, a stabilizing solution is added, ultrasonic treatment is performed after uniform stirring, the fermentation substrate is obtained, and the stabilizing solution is an aqueous solution of a compound of sulfydryl-beta-cyclodextrin and chitosan; primary fermentation: inoculating a microbial bacterial liquid A for anaerobic fermentation, then filtering and collecting filtrate, the microbial bacterial liquid A being obtained by respectively inoculating bacillus velezensis and bacillus coagulans into a culture medium A for shake cultivation and activation and then mixing the bacillus velezensis and the bacillus coagulans; secondary fermentation: inoculating the filtrate with a microbial bacterial liquid B for anaerobic fermentation again, wherein the microbial bacterial liquid B is obtained by inoculating enterococcus faecium into a culture medium B for culture; and extracting. According to the method, the stabilizing solution is added into the walnut green seedcase, and two-stage fermentation is performed, so that the yield and the conversion rate of urolithin A are remarkably improved, and the method is safe, environmentally friendly and capable of being industrialized.

The present invention discloses a process for preparing lactic acid by mechanically enhanced ammonia method from straw-like biomass. The enzymatic hydrolysate of solid fibers obtained by the mechanically enhanced ammonia method from straw-like biomass is used as the carbon source of the fermentation medium in the process of fermenting lactic acid. The preparation of the carbon source in the fermentation medium comprises the following steps: S1: treating straw-like lignocellulosic biomass with a pretreatment liquid containing ammonia water and inorganic ammonium salts in a twin-screw device; S2: subjecting the obtained material to a heat preservation reaction, and after solid-liquid separation, obtaining solid fibers containing holocellulose; S3: enzymatically hydrolyzing the obtained solid fibers with Trichoderma reesei cellulase and xylanase, and performing solid-liquid separation, and the obtained supernatant is used as the carbon source in the fermentation medium for fermenting L-lactic acid. The present invention uses the sugar liquid obtained by enzymatic hydrolysis and saccharification of pretreated holocellulose, inoculates Bacillus coagulans to ferment L-lactic acid after supplementing nitrogen source and inorganic salts, and can greatly improve the concentration and conversion rate of L-lactic acid.

The invention relates to application of bacillus coagulans BC99 in preparation of a probiotic preparation for assisting in reducing the uric acid level. The bacillus coagulans BC99 is a bacillus coagulans BC99 strain of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 21801, and the bacillus coagulans BC99 strain of which the preservation number is CGMCC No. 21801. The invention finds that the bacillus coagulans BC99 has a very remarkable effect in the aspect of assisting in reducing uric acid, and the effect is specifically shown as follows: the levels of serum uric acid, urea nitrogen and creatinine of a hyperuricemia rat are reduced; the xanthine oxidase activity of the hyperuricemia rats is improved; the serum inflammatory factor level of the hyperuricemia rats is regulated, and the inflammatory reaction caused by uric acid increase is inhibited; and the levels of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase of high-uric-acid rats Meanwhile, the bacillus coagulans BC99 is used as a probiotic, so that the product is high in safety and not easy to generate resistance.

The invention relates to a probiotic preparation for improving intestinal health of pets and application of the probiotic preparation. The bacterial strains in the probiotic preparation consist of a bacillus coagulans BC66 bacterial strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 21879, a pediococcus acidilactici PA153 bacterial strain with the preservation number of CCTCC NO: M 2023230, a lactobacillus plantarum LP194 bacterial strain with the preservation number of CGMCC 21766, a bifidobacterium animalis BA20 bacterial strain with the preservation number of CGMCC No. 21830 and a lactobacillus reuteri LR108 bacterial strain with the preservation number of GDMCC No: 63095. According to the probiotic preparation, five specific probiotics are compounded, the five strains are matched and promoted with one another, and synergistic interaction is achieved on the effect of improving the intestinal health of pets, specifically, the digestive ability of the pets on nutrients is improved; the immunity of pets is improved and the diarrhea rate is reduced; the content of beneficial bacteria in intestinal tracts of pets is increased.

The invention relates to the technical field of bio-fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula as well as a preparation method and application of the bio-organic fertilizer. The bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula provided by the invention comprises a kitchen fermentation base material and a complex microbial inoculant in a weight ratio of 100: (1-10), the complex microbial inoculant comprises one or more of bacillus atrophaeus, bacillus coagulans, bacillus licheniformis, bacillus velezensis and brevibacillus laterosporus; the invention further provides a preparation method of the bio-organic fertilizer and application of the bio-organic fertilizer in prevention and treatment of root rot of codonopsis pilosula. The bio-organic fertilizer provided by the invention can degrade glyphosate in soil on one hand, and also can reduce the occurrence of root rot of codonopsis pilosula on the other hand, so that the growth of codonopsis pilosula and the accumulation of active value components of codonopsis pilosula can be promoted.

The invention provides a method for preparing a protease beverage by fermenting cyperus esculentus powder and a product thereof. Comprising the steps of directional pretreatment, fermentation medium preparation, segmented fermentation and optional blending, specifically, directional pretreatment adopts low-temperature extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the fiber structure and antinutritional factors of soybean meal are effectively broken; in the fermentation stage, lactobacillus plantarum, saccharomyces cerevisiae and bacillus coagulans composite flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the system is reduced to 4.0-4.5, and then L-cysteine hydrochloride-vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the protease activity is larger than or equal to 320 U / mL, the total number of viable bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the human gastrointestinal tract environment, and the method has wide industrialization prospects.

The invention discloses a fermented feed prepared from traditional Chinese medicine residues as well as a preparation method and application of the fermented feed. The fermented feed is prepared by mixing and fermenting a mixed bacteria solution formed by fermenting bacillus subtilis, bacillus coagulans, phaffia rhodozyma and lactobacillus plantarum with bran, soybean meal, rice husks, zeolite powder and honeysuckle dregs. The fermented feed disclosed by the invention increases the contents of microbial protein, lactic acid, ethanol, tartaric acid, acetic acid and lactic acid bacteria, contains various fermentation substances, can improve the appetite of animals and improve the palatability, and is high in nutritional value. Honeysuckle herb residues are used as main raw materials, so that the preparation method has the characteristic of low production cost, and the problem of environmental pollution caused by random discarding of herb residue wastes is solved.

The invention relates to a composition for regulating intestinal microenvironment and relaxing bowel and a preparation method thereof, and belongs to the technical field of biology. The composition comprises the following components in specific parts: artichoke-siraitia grosvenorii-fructus cannabis composite extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, escherichia coli bacteriocin, salmonella bacteriocin, a liquorice extract, a semen cassiae extract, a herba cistanche extract, a dendrobium officinale extract and the like. Wherein the artichoke-siraitia grosvenorii-fructus cannabis compound extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, the liquorice extract, the semen cassiae extract, the herba cistanche extract and the dendrobium officinale extract are obtained by a special preparation method. Through cooperation of the components, the intestinal microenvironment can be adjusted from multiple angles, and the comprehensive effects of improving the intestinal flora structure, inhibiting harmful bacteria, promoting the growth of beneficial bacteria, lubricating the intestinal tract, relieving intestinal inflammation, enhancing the intestinal mucosal barrier function, improving the intestinal immunity and the like are achieved.

The invention discloses Witzia ciliaris NCU-33 and application thereof, and belongs to the technical field of microbial medicines. The bacillus coagulans NCU-33 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus coagulans NCU-33 is CGMCC NO.31779. The strain is separated from a fresh excrement sample of healthy people; the strain is capable of ameliorating and / or preventing rheumatoid arthritis by anti-inflammatory and immunomodulatory effects.

The invention provides a compound feed for promoting beef cattle growth and improving meat quality and a production method thereof, and belongs to the technical field of feed preparation. The compound feed comprises the following components in parts by weight: 100 parts of basal feed, 7-10 parts of a functional additive and 0.3-0.5 part of disodium inosinate, and the functional additive comprises a postbiotic compound, a fatty acid regulating agent, a bacterium enzyme synergist and trace elements, wherein the metagen complex comprises a lactobacillus plantarum metagen extract and a bacillus coagulans metagen extract, the fatty acid regulating agent comprises conjugated linoleic acid glyceride and medium-chain fatty acid, and the bacterial enzyme synergist comprises beta-mannase and bacillus subtilis. According to the compound feed, the growth performance and the meat quality can be synchronously improved, and a reliable processing technical guarantee is provided for improving the growth performance and the meat quality of beef cattle.

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.

The invention relates to the technical field of mine restoration, in particular to an ecological restoration method for a mine slope. Slope surface dangerous rocks and sundries of the mine side slope are cleaned, and the cleaned side slope is obtained; spraying and seeding a planting matrix on the cleaned side slope; the planting substrate comprises the following components in parts by weight: 3-8 parts of a water-retaining agent, 15-20 parts of plant straws, 5-10 parts of coconut shells, 5-10 parts of peanut meal, 10-20 parts of a decomposed organic fertilizer, 5-10 parts of a microbial agent, 10-20 parts of rural soil, 10-20 parts of diatomite and 5-10 parts of vermiculite; the microbial agent contains bacillus licheniformis, bacillus flexus, bacillus marinus and bacillus coagulans; plant seeds are sowed on the planting substrate. By adopting the method for repairing the side slope, heavy metal in soil can be removed, the side slope can be greened, and the side slope is gradually covered year by year.

The invention relates to the technical field of functional microbial preparations, in particular to a biological preparation for relieving intestinal discomfort caused by alcohol and a preparation method and application thereof.The main technical scheme is that the biological preparation is prepared from, by weight, 5-20 parts of bacillus coagulans, 10-30 parts of pediococcus pentosaceus, 5-10 parts of lactobacillus rhamnosus and 0-20 parts of saccharomycetes. 0-5 parts of bifidobacterium longum and 0-5 parts of lactococcus lactis. By constructing an alcohol metabolism-intestinal protection dual-function synergistic system, specific florae such as bacillus coagulans, pediococcus pentosaceus and lactobacillus rhamnosus are scientifically compatible with functional components such as inulin and L-cysteine, alcohol is efficiently metabolized, and a carbon source is generated; the technical problems of low metabolic efficiency, weak intestinal protection and serious gastric acid inactivation of a traditional product are comprehensively solved.

The invention discloses active probiotic soft sweets capable of improving intestinal health and enhancing immunity and a high-temperature preparation method of the active probiotic soft sweets. The method comprises the following steps: preparing soluble carbohydrate into an adapter solution with the water activity of 0.7-0.9, the pH value of 5.0-7.0 and the temperature of 40-50 DEG C; adding a certain amount of bacillus coagulans powder into the adaptive solution, and keeping the temperature at 40-50 DEG C for 20-50 minutes to prepare a pre-activation solution; preparing a sugar glue solution; auxiliary materials are added into the carbohydrate gum solution to prepare a blended carbohydrate gum solution, the pH value of the blended carbohydrate gum solution is 3.0-6.0, and the temperature is 60-80 DEG C; adding the pre-activated solution into the blended carbohydrate glue solution, and preserving heat for 10-40 minutes at the temperature of 60-80 DEG C to passivate; and pouring the passivated sugar glue solution into a soft sweet mold at 65-85 DEG C for shaping, and drying until the moisture is 16-18% to obtain the active probiotic soft sweet. The active probiotic soft sweets prepared by the preparation method disclosed by the invention are high in survival rate of probiotics, can keep stable in the delivery period, and have the activity of improving intestinal health and enhancing immunity.

The invention discloses ice clara health-care tea and a preparation method thereof, and belongs to the technical field of health-care tea. The ice clara health-care tea comprises an ice clara extract, an aloe extract, an American ginseng extract, composite ferment powder and composite enzymolysis powder, the composite ferment powder is obtained by fermenting a schisandra chinensis extract, an acanthopanax senticosus extract and a folium cortex eucommiae extract through a composite fermentation inoculant and drying; the compound fermentation inoculant comprises clostridium butyricum, bacillus coagulans and aspergillus oryzae; the compound enzymolysis powder is obtained by mixing radix polygonati officinalis, burdock and echinacea purpurea and then performing enzymolysis with compound enzyme; the compound enzyme comprises pectinase, acid protease, glucose oxidase, malate dehydrogenase and thermolysin. According to the ice clara health-care tea disclosed by the invention, the solubility and bioavailability of the product are improved, and the stability and functionality of the ice clara health-care tea are also enhanced, so that the product has a remarkable advantage in the aspect of improving the immunity, and the product can better meet the requirements of consumers on health care through the elaborately designed process steps.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

The invention relates to the technical field of microbial fermentation and food production, and particularly provides a rhizoma polygonati probiotic fermented beverage and a preparation method thereof. The polygonatum kingianum fermented beverage is prepared by taking polygonatum kingianum as a main raw material and fermenting the polygonatum kingianum with bacillus coagulans, is pure in taste, sour, sweet, tasty and refreshing, and soft in fermentation aroma, contains various nutritional ingredients, has the effects of enhancing immunity, resisting oxidation and the like, has multiple benefits on body health, and is wide in market prospect.