Bacillus subtilis for effectively degrading grease and application thereof

A technology of Bacillus subtilis and Bacillus coagulans, which is applied to Bacillus subtilis and its application in aerobic composting of kitchen waste, can solve the problems of inconvenience in the field and incomparable benefits, and achieves avoiding the decline of microbial activity and efficient degradation. Grease, the effect of reducing the oil content

Active Publication Date: 2017-07-07
HANYU GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology is not convenient for aerobic composting of food waste, and its benefits are not more advantageous than the direct use of chemical surfactants

Method used

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  • Bacillus subtilis for effectively degrading grease and application thereof
  • Bacillus subtilis for effectively degrading grease and application thereof
  • Bacillus subtilis for effectively degrading grease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This example is the isolation, domestication and identification of Bacillus subtilis strain 6-1oil.

[0040] (1) Take 1 g of aerobically fermented food waste compost and dissolve it in 20 mL of sterile water. After fully shaking and dissolving, inoculate the supernatant into the nutrient broth medium according to 5% inoculum size, place it on a shaker, and Cultivate at 37°C and 180 rpm for 48 hours to obtain a culture solution;

[0041] (2) Take the culture solution in step (1) and dilute it until the bacterial solution concentration is 500~1500cfu / mL and spread it on the culture medium containing 1% edible oil (recipe: peptone 10g, beef extract 5g, NaCl 5g, edible oil 10mL, Tween 80 1.5-3mL, agar 15-20g, water up to 1L, pH 7.0-7.4), at a temperature of 37°C, cultivate and screen out a single colony that grows;

[0042] (3) Get the single bacterium colony in the step (2) and inoculate in the liquid culture medium that contains 5% edible oil, cultivate at 37 ℃, 5 days a...

Embodiment 2

[0052] This example is an experiment in which unacclimated Bacillus subtilis and Bacillus subtilis 6-1oil were cultured on high-fat agar plate respectively.

[0053] Prepare solid medium containing edible oil (recipe: peptone 10g, yeast extract 5g, sodium chloride 10g, agar powder 15g, edible oil 50mL, Tween-80 2mL, water up to 1L, pH7.0~7.4), plate Drill holes on top, inoculate unacclimated Bacillus subtilis and Bacillus subtilis 6-1oil respectively, culture them at 37°C, and observe their growth.

[0054] For the growth of the two strains, see Figure 4 ,in Figure 4 The upper and lower holes in the middle are inoculated with unacclimated Bacillus subtilis, and the left and right holes are inoculated with Bacillus subtilis 6-1oil. Depend on Figure 4 It can be seen that Bacillus subtilis 6-1oil grows rapidly on the plate containing edible oil, with a large colony area and surrounds the colonies of unacclimated Bacillus subtilis, indicating that 6-1oil can grow well under ...

Embodiment 3

[0056] This example is a high-fat nutrient solution fermentation experiment

[0057] Inoculate the strain Bacillus subtilis 6-1oil into sterilized oil-containing liquid medium (recipe: peptone 10g, beef extract 5g, sodium chloride 5g, edible oil 10mL, Tween-80 1.5mL, water up to 1L, pH 7.0 ~7.4), cultured on a shaker at 37°C, cultured for 3 days, and observed the results.

[0058] Figure 5A Picture before inoculation of Bacillus subtilis 6-1oil culture for oily liquid medium, courtesy of Figure 5A It can be seen that before the inoculation of bacterial strain Bacillus subtilis 6-1oil, the oily liquid culture medium is yellow, and there is a layer of oil floating on it, and there is a more obvious layering phenomenon, and there is grease attachment on the bottle wall.

[0059] After cultivating for 3 days, the emulsified degradation of the oily liquid medium inoculated with Bacillus subtilis 6-1oil is as follows: Figure 5B shown. Depend on Figure 5B It can be seen that...

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Abstract

The invention provides a bacillus subtilis for effectively degrading grease. The bacillus subtilis 6-loil is preserved at Guangdong Province Microbiological Culture Collection Center on December 22, 2016. The preservation number is GDMCC No. 60134. The bacillus subtilis 6-loil provided by the invention can utilize the grease as a growth carbon source, can normally grow under an environment of higher grease content and has a capacity of efficiently degrading grease. The bacillus subtilis 6-loil provided by the invention can be compounded with bacillus coagulans, brewer yeast and carrier ingredients so as to be prepared into a kitchen garbage disposal fungicide. The prepared kitchen garbage disposal fungicide can adapt to the condition of high grease content of the domestic kitchen garbage, so that the condition of failure in aerobic composting caused by the reduction of microbial activity resulted from the microorganism wrapped by the grease in aerobic composting can be effectively avoided and the bacillus subtilis can meet the requirement of the domestic kitchen garbage aerobic composting. The bacillus subtilis 6-loil also can be applied to the fields of soil oil stain treatment, biosurfactant production, biological detergent preparation, and the like.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a bacillus subtilis capable of efficiently degrading oil and its application in aerobic composting of kitchen waste. Background technique [0002] Food waste refers to the food waste generated in daily life and is one of the important components of urban domestic waste. According to statistics from the Institute of Solid Waste Pollution Control and Recycling of the Department of Environmental Science and Engineering of Tsinghua University, the annual production of kitchen waste in large and medium-sized cities in my country is not less than 60 million tons. Food waste is mainly composed of starch, dietary fiber, protein, and oil. These substances are easily corrupted and mildewed, and the composition is complex. Toxins such as nitrates, toxic and harmful components such as bacteria, and a large number of viruses are not only easy to infect more than 10 kinds of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C11D1/83C11D3/38C12R1/125
CPCA62D3/02B09C1/10C11D1/29C11D1/72C11D1/83C11D3/381C12N1/205C12R2001/125
Inventor 梁嘉亮赵泳婷罗勇
Owner HANYU GRP CO LTD
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