Coagulation bacillus feedstuff supplement and preparation method thereof

A technology of Bacillus coagulans and feed additives, applied in the field of microbial feed additives, can solve the problems of limited application range, single carrier type, inconvenient transportation and so on, so as to avoid the rapid decline of viable bacteria, expand the application range, and have good stress resistance Effect

Active Publication Date: 2009-03-25
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on Bacillus coagulans products is still in its infancy. In the preparation method of Bacillus coagulans for water purification disclosed in patent CN 1831113A, solid fermentation is carried out, and the number of viable Bacillus coagulans prepared is high, reaching 1×10 11 However, due to the shortcomings of solid fermentation method that is easy to contaminate miscellaneous bacteria, researchers began to turn to liquid fermentation method; patent CN 1579200A discloses a method for preparing Bacillus coagulans bacterial agent by liquid fermentation method, and the liquid ferme

Method used

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  • Coagulation bacillus feedstuff supplement and preparation method thereof
  • Coagulation bacillus feedstuff supplement and preparation method thereof
  • Coagulation bacillus feedstuff supplement and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Novel Bacillus Liquid Agent-Y1

[0031] 1) Primary slant culture of Bacillus coagulans (preservation number AS 1.2407):

[0032] Inoculate Bacillus coagulans on the primary slant medium by conventional methods, and culture at 30°C for 24 hours to obtain a single colony of Bacillus coagulans; the medium is a mixture prepared according to the following ratio (g / L) : Soy peptone: 5.0, yeast extract: 15.0, glucose: 3.0, MgSO 4 ·7H 2 O: 0.5,K 2 HPO 4 : 1.0, MnSO 4 : 20ppm, CaCO 3 : 5.0, agar 20; pH: 7.0;

[0033] 2) Secondary liquid culture of Bacillus coagulans

[0034] Inoculate the single colony of the first-level Bacillus coagulans cultured in step 1) into the second-level culture medium by conventional methods, with a liquid volume of 20ml / 500ml, and cultivate it at 35°C and 180rpm / min for 25 hours to obtain the second-level Bacillus coagulans Seed liquid; the medium is a mixed solution (g / L) prepared in the following proportions: soybea...

Embodiment 2

[0039] Example 2 Preparation of Novel Bacillus Liquid Agent-Y2

[0040] 1) Primary slant culture of Bacillus coagulans (preservation number AS 1.2407):

[0041] Inoculate Bacillus coagulans on the primary slant medium by conventional methods, and culture at 40°C for 36 hours to obtain a single colony of Bacillus coagulans; the medium is a mixture prepared according to the following ratio (g / L) : Soy peptone: 15.0, yeast extract: 5.0, glucose: 10.0, MgSO 4 ·7H 2 O: 1.5,K 2 HPO 4 : 4.0, MnSO 4 : 70ppm, CaCO 3 : 15.0, agar 30, pH: 7.0;

[0042] 2) Secondary liquid culture of Bacillus coagulans

[0043] Inoculate the single colony of the first-level Bacillus coagulans cultivated in step 1) into the second-level culture medium by a conventional method, with a liquid volume of 100ml / 500ml, and cultivate it at 45°C and 300rpm / min for 36 hours to obtain the second-level Bacillus coagulans Seed liquid; the medium is a mixed solution (g / L) prepared in the following proportions: ...

Embodiment 3

[0048] Example 3 Preparation of Novel Bacillus Liquid Agent-Y3

[0049] 1) Primary slant culture of Bacillus coagulans (preservation number AS 1.2407):

[0050] Inoculate Bacillus coagulans on the primary slant medium by conventional methods, and cultivate it at 36°C for 30 hours to obtain a single colony of Bacillus coagulans; the medium is a mixture prepared according to the following ratio (g / L) : Soy peptone: 10.0, yeast extract: 10.0, glucose: 5.0, MgSO 4 ·7H 2 O: 1.0, K 2 HPO 4 : 0.4, MnSO 4 : 40ppm, CaCO 3 : 10.0, agar 25, pH: 7.0;

[0051] 2) Secondary liquid culture of Bacillus coagulans

[0052] Inoculate the single colony of the first-level Bacillus coagulans cultured in step 1) into the second-level culture medium by a conventional method, with a liquid volume of 700ml / 500ml, and cultivate it at 36°C and 200rpm / min for 30 hours to obtain the second-level Bacillus coagulans Seed liquid; the medium is a mixed solution (g / L) prepared in the following proportio...

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Abstract

The invention relates to a condensate bacillus feed additive and a preparation method, belonging to the technical field of microbial feed additives. The additive uses one or several of the fish meal, soybean meal, cottonseed meal, urea, ammonium sulfate, diammonium hydrogen phosphate, soybean peptone and yeast extract as the nitrogen source, and also takes one or several of the glucose, lactose, starch, cane sugar, maize flour and bran as the carbon source for fermentation; the number of viable bacteria reaches more than 1 multiplying 109 CFU/ml; after the additive is further concentrated and dried or directly adsorbed to the carrier, bacillus powder is obtained through spray-drying, and the number of viable bacteria reaches more than 1 multiplying 1010 CFU/g. The novel bacillus product produced with the submerged liquid fermentation method provided in the invention is free from toxic side effects, drug resistance and residue; the product can endure processing, intestinal habitat and storage.

Description

technical field [0001] The invention relates to a bacillus coagulans feed additive and a preparation method thereof, belonging to the technical field of microbial feed additives. Background technique [0002] As a substitute for antibiotics, microecological preparations are attracting more and more attention from the feed industry due to their unique function of regulating intestinal flora, and among them, lactic acid bacteria preparations are very popular, with Lactobacillus bifidum and Health products for various animals (including human body) made by Lactobacillus acidophilus and Streptococcus faecalis are extremely popular at home and abroad. However, these preparations use vegetative cells, which have weak resistance to heat and drying, poor preservation, and are not resistant to processing, and the number of viable bacteria drops sharply after the action of gastric acid and bile salts in the digestive tract, which makes the product more durable. Application is limited...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/14C12N1/00C12N1/22A23K10/18A23K20/00
Inventor 张玳华赵雁青莫云宋维平孙占敏姚琨贾秋英白玉卿刘宇赵军
Owner BEIJING DABEINONG TECH GRP CO LTD
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