405results about "Microbiology processes" patented technology

The invention provides a method for preparing rare ginsenoside through conversion of kombucha, the rare ginsenoside and application, and belongs to the technical field of biological medicine. A symbiotic system is formed by multiple bacteria such as acetic acid bacteria, gluconacetobacter, foal bacillus and the like and fungi, after the symbiotic system is domesticated, the American ginseng stem and leaf extract is fermented through multi-bacteria synergistic catalysis, the rare ginsenoside can be efficiently prepared, the highest conversion rate of Rb3 and Rd can reach about 90%, and the yield of Rb3 and Rd can reach about 90%. Meanwhile, the contents of rare ginsenosides Rg3 (20S), Rg5, Rh2 (20R) and Rh2 (20S) are remarkably increased and are increased by about 15 times at most, and efficient conversion of various ginsenosides is achieved.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The invention discloses a domestication method for efficiently degrading tobacco waste to produce hydrogen by using mixed flora, which comprises the following steps: carrying out anaerobic enrichment domestication culture by taking manure compost as a bacterial source and cellulose prepared from tobacco waste as a core component of a domestication culture medium; the domestication culture medium contains cellulose, ammonium bicarbonate and monopotassium phosphate, and the cellulose concentration gradient is gradually increased without adjusting the pH value; stopping culturing when the hydrogen content in the biogas is reduced by 10%, transferring the biogas to a fresh domestication culture medium for passage according to an inoculum size of 10-20%, and continuously domesticating for 20-40 rounds; inoculating the domesticated mixed flora culture solution into a cellulose liquid fermentation culture medium containing microcrystalline cellulose, ammonium bicarbonate, monopotassium phosphate and a nutrient solution according to the inoculum size of 10-20%; the initial pH of the fermentation liquor is adjusted to 6.5-7.5, high-purity nitrogen is introduced to create an anaerobic environment, and hydrogen is produced through fermentation under the conditions of 35 + / -1 DEG C and 120 r / min. According to the method, flora is easy to culture, hydrogen production is efficient and stable, tobacco waste resources can be utilized, flora stability is high, adaptability is high, and strict sterile conditions are not needed.

The invention belongs to the technical field of microorganisms, and particularly discloses high-activity lactic acid bacteria powder as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out activation culture on lactic acid bacteria in a liquid culture medium to obtain an activated bacteria solution; adjusting the concentration of the activated bacterial liquid, standing and incubating, and in the incubation process, dynamically measuring the formation amount of a biological membrane by adopting a crystal violet staining method, and determining the optimal biological membrane incubation time of the lactic acid bacteria strain; adjusting the concentration of the activated bacterial liquid, standing and incubating to the determined optimal biological membrane incubation time; washing off thalli which are not adhered to the upper layer by using the sterile PBS, and centrifugally collecting biofilm state lactic acid bacteria which are adhered to the bottom of the culture container; and re-suspending the collected biofilm state lactic acid bacteria, and drying to obtain the high-activity lactic acid bacteria powder. According to the preparation method, the drying survival rate of the strain is remarkably improved, and the resistance of lactic acid bacteria to stress such as dehydration, heat stress and cold stress in the drying process is effectively enhanced.

The present invention relates to a method for hydrolyzing carbohydrates present in raw materials containing carbohydrates, comprising the step a) of contacting the raw material containing carbohydrate with a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a method for simultaneously liquifying and saccharifying starch, a method for simultaneously liquifying, saccharifying and fermenting starch, and to a method for producing a biomass, the method comprising the step of cultivating bacteria secreting carbohydrate-hydrolyzing enzymes on a medium comprising complex carbohydrates as a major carbon source.

The present disclosure relates to: a method for preparing heat-killed lactic acid bacteria.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The invention relates to a Saccharomyces cerevisiae C16I09 strain which is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the Saccharomyces cerevisiae C16I09 strain is CCTCC (China Center for Type Culture Collection) No. M20251150. The strain disclosed by the invention has good oxidation resistance and can be applied to the fields of skin care products, health care products, anti-aging foods and the like. Especially, extracellular fermentation broth of the strain, namely a fermentation product or a fermentation extract of the strain, has excellent oxidation resistance, can scavenge free radicals and relieve oxidative damage, has the effects of protecting skin, resisting inflammation, delaying senescence and the like, and can be applied to the fields of skin care products, health care products, anti-aging foods and the like as a natural anti-aging agent.

The application discloses a heavy metal resistant bacillus velezensis and a microbial repair agent and application thereof. The bacillus velezensis (Bacillus velezensis DBS01) is screened from heavy metal contaminated soil in Dabaoshan mine area of Shaoguan city in Guangdong province, and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 20252228. The bacillus velezensis not only has multiple heavy metal resistance capabilities, but also can metabolically secrete organic acids, cellulase, growth hormones and other metabolites in different growth stages, so as to realize multiple functions such as heavy metal solidification, phosphorus dissolution, potassium dissolution and plant growth promotion. The bacillus velezensis DBS01 is inoculated into a culture medium containing straws, calcium superphosphate is added, and agar and sodium alginate are added, and the mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the mine heavy metal contaminated soil can be repaired in a high-efficiency, low-cost and environment-friendly manner, and the ecological function recovery of the soil can be effectively promoted.

This invention discloses a method for preparing a carbon source for microbial culture medium based on enzymatic hydrolysis of bamboo powder and its application, belonging to the field of high-value utilization technology of biomass resources. Using bamboo powder as raw material, the invention involves mild alkali pretreatment to disrupt the dense structure of lignocellulose, followed by enzymatic hydrolysis using a specialized enzyme system of endoglucanase Cel7B and xylanase XynA expressed heterologously by *E. coli* in an optimized ratio. After solid-liquid separation, an enzymatic hydrolysate rich in reducing sugars is obtained. The hydrolysate is then nutritionally fortified with trace elements such as sodium thiosulfate and sodium silicate to produce a low-cost, high-efficiency liquid carbon source. This invention achieves high-value utilization of bamboo waste. The entire process is mild, environmentally friendly, and simple, easily scaled up industrially, and solves the industry bottleneck of high carbon source cost in industrial microbial culture, demonstrating good application prospects and market value.

In alternative embodiments, the invention provides vaccines, pharmaceutical compounds and formulations for diagnosing, preventing, treating or ameliorating Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections. In alternative embodiments, the invention provides compositions such as diagnostic tests, assays, immunoassays and test strips, and methods, for detecting or diagnosing the presence of a Streptococcal infection, e.g., Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections.

Provided herein is a method of producing a target product, comprising co-culturing a producer cell and a recycler cell in a culture media comprising a first nutrient source, wherein the producer cell is capable of producing the target product and is capable of producing a second nutrient source; and the recycler cell is capable of producing the target product, is capable of metabolising the second nutrient source, and is not capable of metabolising the first nutrient source. Also provided herein are cells, nucleic acids, methods, and kits associated with the said method of producing a target product.

The present invention relates to a method for cultivating a microorganism, preferably a Saccharomycetales yeast, capable of utilizing ethanol as feedstock. The invention is directed to a method for the production of biomass, in particular single cell protein, wherein the yeast single cell protein product comprises Saccharomycetales yeast cells, as well as to an animal feed comprising such biomass.

Disclosed are cell culture media using microorganismal, e.g., bacterial, fungal, lysates as a replacement for serum that are effective for short-term and long-term cultivation of animal cells, e.g., generation of cultivated meat. Also disclosed are systems for culturing animal cells using the media, methods of making the media, methods of detecting suitable serum replacements, and kits.

The invention belongs to the field of microorganism application, and particularly relates to a bacterium capable of degrading pyrethroid pesticides, and a preparation and application thereof. The invention relates to the field of biotechnology, and in particular relates to a pseudomonas aeruginosa, which is named as paracoccus panotrophus BB-2 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251184, and pseudomonas aeruginosa, which is named as pseudomonas aeruginosa BS-3 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251185. The invention further relates to a mixed bacterial agent of the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3, it is found that the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3 can be subjected to synergistic symbiosis and secrete and produce extracellular hydrolase and multiple oxidoreductases, pyrethroids are efficiently degraded, and the removal efficiency of the multiple pyrethroids reaches 90% or above.

This invention discloses a highly efficient *Bacillus cereus* strain that degrades sulfamethoxazole and its applications, belonging to the field of microbial application technology. The *Bacillus cereus* SDB-X, with accession number CCTCC NO: M2026459, exhibits highly efficient degradation capabilities for sulfamethoxazole. It achieves a degradation rate of over 96% for 50 mg / L SMX within 5 days, and a degradation rate as high as 97.8% in simulated medical wastewater, significantly outperforming most existing degrading strains and filling the technological gap in highly efficient SMX degradation by *Bacillus cereus*. It maintains high degradation performance even in complex medical wastewater environments, is unaffected by complex substrates, and is minimally affected by abiotic factors, demonstrating high practical value.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

Provided herein are methods and systems for treating a stream comprising material (e.g., biomass such as plant biomass, animal biomass, agricultural biomass, wastewater sludge, or a combination thereof) with nanobubbles. One such method includes generating nanobubbles in wastewater sludge of a wastewater treatment system to produce nanobubble-containing sludge, the wastewater sludge having a higher solids content than an input stream to the wastewater treatment system, and processing the nanobubble-containing sludge in an anaerobic reactor of the wastewater treatment system.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

The present invention relates generally to biotechnology techniques, and specifically to genetically modified bacteria of the genus Methylobacillus having improved properties that are particularly useful in large-scale methanol fermentation. More specifically, the present invention provides bacteria of the genus Methylobacillus that have been modified to reduce exopolysaccharide (EPS) production compared to an otherwise identical bacterium lacking the modification. The present invention further provides methods for producing biochemical compounds using the genetically modified bacteria of the present invention.

The application discloses a fishery breeding method and device based on wind and light power generation, equipment and medium, and relates to the technical field of fishery breeding. The method comprises the following steps: acquiring resource data of a breeding project location and power load demand of an algae and fish breeding system, constructing a capacity optimization configuration model according to the resource data and the power load demand, iteratively solving the capacity optimization configuration model to obtain optimal capacity matching based on power generation cost, and delivering power to the algae and fish breeding system according to the optimal capacity matching. The temperature and light intensity are adjusted based on the power and a preset gradient through the algae and fish breeding system, the microalgae strains are domesticated and cultured according to the adjusted temperature and light intensity, the corresponding domesticated microalgae strains are screened based on fishpond wastewater, and target microalgae strains are obtained. The active cell liquid of the microalgae strains is acquired, and the active cell liquid of the microalgae strains is used for water purification and feed supply, so that fishery breeding is realized. The application improves the microalgae so that the microalgae can purify wastewater.

The invention relates to the technical field of microbial fermentation, and particularly discloses a high-density expanding culture method of autotrophic nitrifying bacteria. The method comprises the following steps: screening low-temperature aquatic plants, and carrying out stepped pressurization domestication to obtain low-temperature-resistant and high-salt-resistant autotrophic nitrobacteria strains; a three-stage stepped expanding culture process is adopted, and a carrier filler with a high specific surface area is added in second-stage fermentation and third-stage fermentation; in the fermentation process, continuous collaborative fed-batch feeding, pH linkage regulation and control and dissolved oxygen precise linkage control of an inorganic carbon and nitrogen source are implemented. Through stress-resistant strain screening, carrier adding and precise process control, the density of the thalli in the fermentation liquor can be increased, the stress resistance of the thalli is improved, and the method is suitable for a more complex water body environment.

The application discloses a triazine herbicide propoxur degrading bacterial strain and application thereof, the bacterial strain is SWFU-DH02 strain which is negative in gram staining reaction and is identified as Microbacterium sp. Exiguobacteruim sp. ) on November 22, 2021, and is deposited in the China General Microbiological Culture Collection Center, and the strain preservation number is CGMCC No. 23948. The application discloses, for the first time, the degradation effect of Microbacterium sp. Exiguobacteruim sp. ) on triazine herbicide propoxur, and a Microbacterium sp. high-efficiency degrading bacterium SWFU-DH02 is screened, the degradation efficiency of the bacterium on propoxur can reach 75.08%, and the bacterium grows optimally under the conditions of pH 7.0, temperature 28 DEG C and salt concentration 1%. The degrading bacterial strain SWFU-DH02 has the characteristics of simple culture, rapid growth and high efficiency of degradation. The application can solve the pollution problem of triazine herbicide propoxur in soil or water, prevent the phytotoxicity of herbicide residues on subsequent crops, and produce non-toxic and non-polluting green agricultural products. The application provides a new biological approach for modern pesticide pollution control.

The present disclosure relates to methods, devices and systems for exposing microorganisms or compositions comprising functional proteins to an oscillating electric field substantially free of a magnetic component in order to improve stress responses, survival and metabolite or protein production. In embodiments, a controller drives one or more insulated emitters with a true AC signal or pulsed DC signal, in stepped, swept or shuffled patterns of an electric field oscillating at one or more frequencies in the range 210 Hz to 99 kHz. Exposure to the field provides surmounting energy that enhances protein breathing, catalysis, and fundamental functions of multiprotein complexes including transcription factors and ribosomes, thereby improving stress resistance of microorganisms, their viability during fermentation, drying, storage, transport, and / or increasing production of functional or therapeutic proteins, metabolites or microbial lysates. The technology is applicable to manufacture of probiotics, therapeutics, metabolites, food, alcohol, feed and bioenergy, cosmetics, nutraceuticals and biopharmaceuticals..

The invention discloses a method for producing medium-chain fatty acid through a core-shell structure magnetic polymer material reinforced biological carbon chain lengthening reaction, which comprises the following steps: by taking a perishable organic solid waste as a raw material, adding core-shell structure magnetic polypyrrole, adding a culture medium solution containing a substrate, adding a domesticated inoculum, and carrying out a reaction for 2-3 hours; a fermentation system for producing hexanoic acid through biological carbon chain lengthening is constructed for reaction, the reaction temperature is 30-40 DEG C, and the pH value is weak acidity; according to the magnetic polypyrrole with the core-shell structure, ferroferric oxide is used as a core, and polypyrrole is used as a shell; the problems that single magnetic particles are agglomerated, magnetic components are prone to loss, and traditional materials are difficult to recycle and large in adding amount are solved, and the performance stability and the reaction promoting capacity are enhanced by means of the structural advantages of the materials. Meanwhile, the efficiency of the carbon chain lengthening reaction and the yield of medium-chain fatty acid are remarkably improved, and high-valued conversion of the perishable organic solid waste is achieved.