1194results about "Microbiology processes" patented technology

The invention belongs to the technical field of food microorganisms, and relates to lactobacillus plantarum and application thereof in preparation of products for relieving insomnia. The preservation number of the lactobacillus plantarum is GDMCC No: 65061. The lactobacillus plantarum can be applied to efficient fermentation of spina date seed juice, the content of GABA, polypeptide, total phenols and total flavonoids in the spina date seed juice is increased, and the content of spinosin, 6 ''-feruloyl spinosin, jujuboside A, jujuboside B and betulinic acid is increased; the reduction of whole brain coefficient and the increase of liver coefficient caused by long-term insomnia are relieved, the bad condition caused by long-term insomnia in a hippocampus DG region in the brain of the mouse is improved, the sleep time of the mouse is prolonged, and the insomnia symptom of the mouse is relieved; the content of inhibitory neurotransmitters 5-hydroxytryptamine, melatonin and gamma-aminobutyric acid in the hypothalamus of the mouse is increased, the content of excitatory neurotransmitter glutamic acid is reduced, and the reduction of organism immunity and oxidative stress of brain tissues caused by insomnia are relieved.

The invention relates to a preparation method of a heterotrophic nitrification-aerobic denitrification bacterial agent and an obtained HNAD bacterial agent. The preparation method comprises the following steps: obtaining a heterotrophic nitrification denitrification strain; mixing the obtained heterotrophic nitrification nitrogen removal strain with an aerobic denitrification bacterium solution to obtain a compound HNAD (Heterotrophic Nicotinamide Adenine Dinucleotide) flora; and inoculating the obtained compound HNAD flora into a bio-membrane reactor, constructing a stable biofilm culturing HNAD bio-membrane reactor, and domesticating and enriching to obtain the HNAD microbial inoculum. The invention also provides an application of the HNAD microbial inoculum in nitrogen-polluted rare earth mine groundwater remediation. The HNAD microbial agent disclosed by the invention has a remarkable denitrification effect and a wide application prospect on nitrogen-polluted rare earth mine underground water, and has important significance on improving the ecological environment, and the HNAD microbial agent and the remediation method disclosed by the invention have important significance on protecting underground water resources.

The invention discloses a breeding method of high-concentration cane molasses tolerant robust saccharomyces cerevisiae and high-concentration ethanol fermentation application of the high-concentration cane molasses tolerant robust saccharomyces cerevisiae, and belongs to the technical field of biology. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on July 25, 2023, and the number of the Saccharomyces cerevisiae NGTM-F1 is GDMCC 63687. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is named as Saccharomyces cerevisiae NGTM-F1. The saccharomyces cerevisiae is obtained by taking laboratory saccharomyces cerevisiae GJ08 as an original strain, carrying out ARTP (room temperature plasma) mutagenesis and adaptive evolution breeding and utilizing high-throughput screening, keeps a good growth state in cane molasses with the total sugar being 300 g / L, and has good fermentation performance in cane molasses with the total sugar being 250 g / L at the temperature of 30 DEG C. Under the condition of 30 DEG C in a conical flask, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 102.30 g / L, and the fermentation efficiency is 80.8%; under the condition of 30 DEG C in a 5 L fermentation tank, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 111.65 g / L, and the fermentation efficiency is 87.0%.

The embodiment of the invention discloses plant lactobacillus, selenium-enriched lactic acid bacteria and application of the plant lactobacillus and the selenium-enriched lactic acid bacteria in preparation of selenium-enriched food, the plant lactobacillus is inoculated into a sodium selenite solution, and the selenium-enriched lactic acid bacteria are obtained through culture. Adding the selenium-rich lactic acid bacteria into the pickled bamboo shoot brine containing the pickled bamboo shoots, and fermenting to obtain the selenium-rich fermented pickled bamboo shoots. The plant lactobacillus with high selenium conversion rate is obtained, the prepared selenium-enriched lactic acid bacteria have wide application, the selenium-enriched lactic acid bacteria can be applied to preparation of selenium-enriched food, the selenium content in the food is greatly increased, for example, the physicochemical properties of the selenium-enriched fermented pickled bamboo shoots prepared in the invention are improved compared with those of traditional pickled bamboo shoots, and the selenium-enriched fermented pickled bamboo shoots have good market prospects. Good oxidation resistance is realized.

The invention relates to talaromyces purpurogenus D2 and application thereof, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D2 disclosed by the invention has the preservation number of GDMCC (Graphics Decific Microorganism Center) NO.66320 The strain is obtained by being separated from soil and domesticated, the zinc tolerance concentration of the strain can reach 2g / L, and the strain can efficiently convert inorganic zinc into organic active zinc which can be easily absorbed and utilized by a human body. The organic zinc prepared from the strain has a remarkable ABTS free radical scavenging capability, and also has tyrosine kinase and 5-alpha reductase inhibition capability; the organic zinc prepared from the talaromyces purpurogenum D2 can be used for preparing zinc-supplementing health-care products, can also be used as cosmetic active matter raw materials with free radical scavenging and whitening effects, and has a wide market application prospect.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention discloses a sulfur disproportionation-mediated low-temperature high-efficiency low-carbon denitrification and synchronous ammonium recovery method for sewage, belongs to the field of sewage treatment, and aims to solve the problems that an existing autotroph denitrification process cannot adapt to water quality fluctuation and seasonal variation and is poor in engineering applicability. Polyculture denitrification and elemental sulfur disproportionation (SD) are used as an effective enhanced denitrification scheme and are applied to a low-temperature SDAD system of actual sewage. Sulfide and polysulfide generated in situ through elemental sulfur disproportionation reaction and low-concentration sodium acetate under the polyculture condition are used as supplementary electron donors, so that the denitrification rate is remarkably increased. The process of reducing dissimilatory nitrate into ammonium (DNRA) in the SD-MixBNR system is beneficial to emission reduction of greenhouse gases while realizing ammonium recovery. According to the method, the SD-MixBNR system can be promoted to be widely applied to actual engineering projects under the carbon neutralization background, and the stability and adaptability of the SD-MixBNR system in the face of various water quality fluctuations and seasonal changes are enhanced.

The invention relates to bacillus pumilus and application thereof. The bacillus pumilus DX-fK is obtained through a chemical mutagenesis method, the bacillus pumilus DX-fK can efficiently degrade straw, the bacillus pumilus DX-fK is used as a substrate to produce feed alternative protein, and therefore the bacillus pumilus DX-fK can be applied industrially and has good industrialization prospects.

The invention belongs to the technical field of in-vitro cultivation of calculus bovis, and particularly relates to a method for in-vitro cultivation of calculus bovis. According to the invention, firstly, the escherichia coli is subjected to bile domestication and ray induced mutation, a high-activity oxgall-resistant bezoar production engineering bacterium E.coli-NH01 is obtained through screening, a method for in-vitro cultivation of bezoar by using the engineering bacterium E.coli-NH01 is provided, the prepared bezoar meets the pharmacopoeia standard, the production efficiency of in-vitro cultivation of bezoar is improved, and the production cost is reduced. And the production cost is reduced, the overall forming period is shortened, and the key that precious traditional Chinese medicinal materials are popular can be achieved.

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention provides an inoculum domestication method for relieving acid or ammonia nitrogen inhibition of an anaerobic fermentation system, which comprises the following steps of: carrying out constant-temperature anaerobic fermentation culture at medium temperature (30-35 DEG C) or high temperature (50-55 DEG C) until the gas yield is stable by taking livestock manure, activated sludge, industrial anaerobic digestion residues or mineralized refuse biogas fermentation liquor and the like as inoculums; the obtained inoculum is an inoculum which has relatively strong acid tolerance or ammonia nitrogen tolerance after domestication. The domesticated inoculum with good tolerance and the original untreated inoculum are mixed according to a certain proportion and then put into an anaerobic fermentation system, and wet fermentation (TS = 5-10% and total solid content) or dry fermentation (TS = 20-30%) is carried out, so that the pH value of the anaerobic fermentation system is recovered to a state suitable for growth of methanogens.

The invention provides a Burkholderia thailantensis DIS2 bacterium, a Burkholderia thailantensis DIS2.1 bacterium, a bacterium with all recognition characteristics of the Burkholderia thailantensis DIS2 bacterium and the Burkholderia thailantensis DIS2.1 bacterium, and a mutant of the Burkholderia thailantensis DIS2 bacterium and the Burkholderia thailantensis DIS2.1 bacterium. The invention also provides a Starmerella bombicola DIS4 yeast, a yeast with all recognition characteristics of the Starmerella bombicola DIS4 yeast, and a mutant of the Starmerella bombicola DIS4 yeast. Also provided are methods for producing rhamnolipids and methods for producing sophorolipids. In order to produce rhamnolipids, raw materials comprising waste carbon sources and fried edible oil are fermented with Burkholderia Thailand, mutants or variants thereof. Similarly, in order to produce sophorolipids, a raw material comprising a waste carbon source and a deep-fried edible oil is fermented with Starmomyces sp., a mutant or a variant thereof.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The present invention relates to a modified bacterium with anti-tumor activity, which comprises an essential gene expression cassette under the control of a strictly hypoxia inducible promoter and is deficient in at least one gene involved in or regulating the endogenous anti-oxidative stress response pathway or a functional expression product thereof. The present invention also relates to a pharmaceutical composition comprising the modified bacterium and anti-tumor use thereof.

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

The invention discloses a phosphate solubilizing bacterium with eurytopic characteristic and application thereof, the bacterium is separated from corn straw biochar in which rice is continuously planted for 3 years, is identified as Pseudomonas paaversuta and is named as MPP2401, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuchang District, Wuhan, Hubei province, the preservation date is November 6, 2024, and the preservation number is CCTCC NO: M20242439. The strain can keep stable phosphate solubilizing capacity under the environmental conditions of 15-30 DEG C, pH of 7-10 and NaCl 0-0.8 mol.L <-1 >, and still has functional phosphate solubilizing capacity even under the condition of extreme low temperature of 5 DEG C, the composite stress of pH of 7-8 and NaCl 0-0.8 mol.L <-1 > and the composite stress of pH of 9-10 and NaCl 0-0.6 mol.L <-1 >. Meanwhile, the bacterium has the broad-spectrum antibacterial characteristic, and the antibacterial rate on fusarium graminearum, fusarium verticillioides and magnaporthe oryzae reaches 41.0-77.6%. Due to the unique wide environment adaptability and the phosphate solubilizing-bacteriostasis dual-function synergistic characteristic, a microbial germplasm resource with production-ecology dual values is provided for biochar bacterial fertilizer development, saline-alkali phosphorus activation and biological prevention and control.

The invention belongs to the technical field of microorganisms, and particularly relates to streptomyces cinnamomi, a fungicide, a method for synthesizing monensin and application. The invention provides streptomyces cinnamomi SDSL6002, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.30409. The streptomyces cinnamomi SDSL6002 provided by the invention has the advantages that the capability of simultaneously metabolizing glucose and xylose is obviously improved, and monensin can be synthesized by simultaneously utilizing the xylose and the glucose. The invention further provides a method for synthesizing the monensin or increasing the yield of the monensin, it is verified that the streptomyces cinnamomi SDSL6002 can be used for synthesizing the monensin through bagasse enzymatic hydrolysate, and the yield of the synthesized monensin is high.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The preparation method comprises the following steps: centrifuging an activated culture solution of animal bifidobacterium subsp. Lactis HNU212 to remove a supernatant, washing with 0.9% normal saline, adjusting the strain concentration to 1 * 10 < 9 > CFU / mL, and inactivating at 90 DEG C for 15 minutes, thereby obtaining the thermal inactivation bifidobacterium subsp. Lactis metagen. The invention also provides a preparation method of the thermal inactivation bifidobacterium subsp. Lactis metagen, and the preparation method of the thermal inactivation bifidobacterium subsp. Lactis metagen comprises the following steps of: preparing the thermal inactivation bifidobacterium subsp. Lactis metagen and the preparation method of the thermal inactivation bifidobacterium subsp. Lactis metagen. When applied to an HUA animal model, the thermal inactivation substance bifidobacterium subsp. Lactis metaplasm prepared by the invention can effectively reduce the serum uric acid level, relieve kidney and intestinal injury and regulate host intestinal flora, and has the effect of relieving and treating HUA.

The invention discloses a saline-alkaline tolerant compound microbial agent and application thereof, and particularly relates to the technical field of microbial soil treatment. The microbial agent comprises Klebsiella, Enterobacter hormaechei and humic acid, the ratio of the components is as follows: Klebsiella: Enterobacter hormaechei: humic acid = 1: (0.5-2): (1-3), and both Klebsiella and Enterobacter hormaechei are obtained by fermentation culture. According to the present invention, the specific pH gradient is adopted for the Klebsiella and the Enterobacter hormaechei, and the segmented fermentation process combining the specific pH gradient with the corresponding gradually-increased sodium chloride concentration is adopted, such that the strain is effectively subjected to the directional domestication so as to maintain the activity and the function in the high-salt and high-alkali extreme environment.

The present invention belongs to the field of functional microbial application technology, specifically a strain of Bacillus licheniformis for efficient biosynthesis of nano-selenium, its application and a green method for preparing nano-selenium. Bacillus licheniformis B1-Se for efficient biosynthesis of nano-selenium has been deposited in the Guangdong Provincial Microbial Culture Collection Center on June 27, 2024, with a deposit number of GDMCC NO: 64806. The present invention uses a method of specific screening and directional domestication by gradually increasing the concentration of sodium selenite, from wild strains to efficient engineered strains, so that it can stably tolerate a sodium selenite concentration environment of up to 6600 mg / L, and the sodium selenite conversion rate is increased to 92.19%, and the nano-selenium production is increased to 2778.05 mg / L, reaching the international leading level.

The invention discloses a method for efficiently leaching waste battery positive electrode powder by using hydrogel to assist bacteria, belonging to the field of resource recovery technology. The invention utilizes hydrogel to assist bacteria to leach valuable metal elements from waste LIBs. The bacteria include L. plants and / or L. acidophilus , the hydrogel is prepared by polyvinyl alcohol and agarose. The present invention creatively applies hydrogel in the bioleaching process, effectively increases the leaching percentage of valuable metals in waste LIBs, and avoids the use of inorganic acid at the same time. Compared with the traditional chemical leaching technology, the method of the present invention has obvious advantages in operational safety and effectively avoids the risk of secondary pollution to the environment. By using microorganisms, not only the efficient recovery of metal resources in the battery is achieved, but also the environmental friendliness of the whole process is ensured.

The invention relates to bacillus velezensis LYB-S and application thereof in improving the reduction rate of hexavalent chromium in combination with exosomes, and belongs to the technical field of environmental restoration. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC NO.34429. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC). The bacillus velezensis LYB-S can be combined with an exosome, so that the reduction rate of the hexavalent chromium can be increased together with the bacillus velezensis LYB-S.

The invention discloses a streptococcus suis type 2 gene deletion strain as well as a construction method and application thereof, and belongs to the technical field of biological products. According to the invention, the B9H0105210 gene in the genome of the streptococcus suis serotype 2 SC19 is deleted in a homologous recombination manner for the first time, the hydrogen peroxide and nitric oxide stress resistance of the constructed streptococcus suis serotype 2 gene deleted strain is reduced, and the pathogenicity to mice is obviously weakened; moreover, the growth performance of the streptococcus suis type 2 gene-deleted strain is not greatly different from that of a wild strain SC19, the condition of gene recovery does not exist after continuous passage, and the streptococcus suis type 2 gene-deleted strain has an application prospect as an attenuated live vaccine, has a good immune protection effect on different genotypes of streptococcus suis such as the streptococcus suis type 2 strain SC19 and streptococcus suis type 2 P1 / 7, and has a good application prospect. And a certain cross immune protection performance is achieved.

The invention provides N-Fe co-doped biochar, a preparation method and application of the N-Fe co-doped biochar in production of medium-chain carboxylic acid by anaerobic digestion. The preparation method comprises the following steps: S1, pretreating a nitrogen-rich biomass raw material; s2, pyrolyzing and carbonizing the pretreated nitrogen-rich biomass raw material to obtain N-doped biochar; s3, adding FeCl3. 6H2O, a stabilizer, a buffer agent and N-doped biochar into an organic solvent, and uniformly mixing to obtain a mixed solution; s4, performing microwave-assisted solvent heat treatment on the mixed solution to obtain a precursor mixed solution; and S5, carrying out centrifugal washing on the precursor mixed solution, filtering, and drying to obtain the N-Fe co-doped biochar. On one hand, the required nitrogen element is obtained by using the nitrogen-rich biomass raw material, and the use of an exogenous nitrogen-containing material is avoided; on the other hand, the biochar is kept in a good dispersion state in a solution through microwave solvent heat treatment, agglomeration of particles is avoided, the electrical conductivity, the thermal conductivity and the mechanical strength of the biochar are improved, and the N-Fe co-doped biochar can also be used for producing medium-chain carboxylic acid through anaerobic digestion.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The present disclosure belongs to the technical field of microorganisms, and specifically relates to a non-toxin-producing Aspergillus flavus strain, a microbial inoculant, a preparation, and use. The present disclosure provides a non-toxin-producing Aspergillus flavus strain EXY1A109 with a deposit number of CCTCC No: M20221465. The strain can effectively inhibit the production of aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 by a toxin-producing Aspergillus flavus strain. The results of examples show that when an Aspergillus flavus strain EXY1A109 spore suspension and a strain CGMCC 3.4408 spore suspension are mixed in an equal volume, the Aspergillus flavus strain EXY1A109 spore suspension and the strain CGMCC 3.4408 spore suspension each have a concentration of 1×106, and the strain EXY1A109 has a toxin-producing inhibition rate of 99.71% against the strain CGMCC 3.4408. This Aspergillus flavus strain shows a desirable application effect.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.