663results about "Microbiology processes" patented technology

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention discloses a saline-alkaline tolerant compound microbial agent and application thereof, and particularly relates to the technical field of microbial soil treatment. The microbial agent comprises Klebsiella, Enterobacter hormaechei and humic acid, the ratio of the components is as follows: Klebsiella: Enterobacter hormaechei: humic acid = 1: (0.5-2): (1-3), and both Klebsiella and Enterobacter hormaechei are obtained by fermentation culture. According to the present invention, the specific pH gradient is adopted for the Klebsiella and the Enterobacter hormaechei, and the segmented fermentation process combining the specific pH gradient with the corresponding gradually-increased sodium chloride concentration is adopted, such that the strain is effectively subjected to the directional domestication so as to maintain the activity and the function in the high-salt and high-alkali extreme environment.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

The invention provides a method for preparing rare ginsenoside through conversion of kombucha, the rare ginsenoside and application, and belongs to the technical field of biological medicine. A symbiotic system is formed by multiple bacteria such as acetic acid bacteria, gluconacetobacter, foal bacillus and the like and fungi, after the symbiotic system is domesticated, the American ginseng stem and leaf extract is fermented through multi-bacteria synergistic catalysis, the rare ginsenoside can be efficiently prepared, the highest conversion rate of Rb3 and Rd can reach about 90%, and the yield of Rb3 and Rd can reach about 90%. Meanwhile, the contents of rare ginsenosides Rg3 (20S), Rg5, Rh2 (20R) and Rh2 (20S) are remarkably increased and are increased by about 15 times at most, and efficient conversion of various ginsenosides is achieved.

The present invention relates to a method for producing a mycelium sheet. The invention discloses a method for producing a mycelium sheet, which comprises the following steps of: standing and culturing fungi in a liquid culture medium containing cellulose, so that the mycelium sheet is formed on the surface of the liquid culture medium; and collecting the mycelium sheet from the surface of the liquid culture medium containing cellulose.

The invention discloses a strain of saccharomyces cerevisiae CPJ-02 with high acid resistance, high ethanol content and strong ester aroma generation and application of the saccharomyces cerevisiae CPJ-02, the strain of the saccharomyces cerevisiae CPJ-02 has been preserved in China Center for Type Culture Collection on June 27, 2024; the preservation number of the strain is CCTCC NO: M 20241388. The saccharomyces cerevisiae CPJ-02 strain is milky white to faint yellow after being cultured on a YPD solid plate for 48 hours, the surface is dry and matte, the texture is slightly rough, and the colonial morphology is round. The saccharomyces cerevisiae CPJ-02 strain has prominent high ethanol tolerance, and still has activity greater than or equal to 92% in a 12% ethanol environment; and the fermentation product is rich in aroma and high in ethyl caprylate and phenethyl alcohol content, more than or equal to 25 volatile substances are detected by GC-MS (gas chromatography-mass spectrometry), and the fermented product is rich in fruit fragrance and has ester flower fragrance. The saccharomyces cerevisiae CPJ-02 strain is suitable for production of high-concentration wines and directional production of low-concentration healthy fermented beverages, fermented foods and aroma compounds.

The invention discloses a method for leaching waste lithium battery positive electrode powder by using intestinal flora in a neutral environment, and belongs to the technical field of resource recycling, the method comprises the following steps: step 1, a pretreatment link, the pretreatment link comprises the following process links, and each link has no sequence: (1) preparation of battery powder, (2) acquisition of intestinal flora, and (3) preparation of a culture medium; 2, domesticating the flora; and 3, valuable metal in the waste battery powder is leached out. According to the method, the intestinal flora is applied to the biological leaching of the waste LIBs positive electrode powder, so that the dependence of traditional strong acidophilic bacteria on an extremely low pH condition is broken through, and the environmental harm is greatly reduced; according to the method, inorganic acid does not need to be added, the pH value of a reaction system is stabilized within the nearly neutral range of 7.8-8.2, equipment loss caused by acid etching is reduced, reagent consumption for subsequent metal recovery and neutralization treatment is reduced, and remarkable economic benefits and environmental benefits are achieved.

A method for preparing a microbial consortium composite remediation agent for synergistic treatment of PAEs and Cd. The preparation method comprises: preparing a functional microbial consortium; preparing a microbial consortium carrier; and preparing the microbial consortium composite remediation agent. In the involved microbial consortium composite remediation agent, the functional microbial consortium is subjected to cadmium-tolerance acclimation, thereby not only exhibiting broad-spectrum degradation activity against PAEs, but also overcoming the issue of reduced degradation efficiency of single functional strains under the stress of heavy-metal Cd pollution in composite contaminated soil. The involved composite remediation agent is modified with sulfhydryl groups, providing excellent passivation effects on heavy metals such as cadmium. In combination with the functional microbial consortium, the remediation agent can achieve the effect of removing PAEs and Cd from the soil synchronously.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention relates to the technical field of soil improvement and microorganisms, and discloses a preparation process of a porous activated carbon soil conditioner loaded with microbial flora, which comprises the following steps: firstly, performing high-temperature activation and acid-base modification on a biomass raw material to prepare a porous activated carbon carrier, and dipping in a nutrient solution to preset a slow-release nutrient substrate; secondly, adopting a step-by-step sequential loading process, firstly loading the bacillus subtilis subjected to stress resistance domestication on a carrier through electrostatic adsorption, and performing activation colonization culture to form a stable pioneer biological membrane; then, on the basis of the biological membrane, phosphate solubilizing bacteria, potassium solubilizing bacteria and antagonistic bacteria are loaded in sequence; finally, the loaded composite carrier is subjected to microcapsule embedding and low-temperature drying treatment, and the porous activated carbon soil conditioner is obtained. According to the modifier prepared by the invention, through ordered loading and multiple protection, the problem of strain competition is solved, the high survival rate of flora is ensured, and the improvement effect is stable and long-acting.

The application discloses a microbial electrochemical system device and method for realizing wastewater treatment, CO2 fixation and in-situ synthesis of microbial protein synchronously, which comprises an anode chamber, a cathode chamber, a protein production chamber, a power supply, a mixed gas tank and a peristaltic pump; the anode chamber, the cathode chamber and the protein production chamber are sequentially arranged from left to right; a cation exchange membrane is arranged between the anode chamber and the cathode chamber; a cation exchange membrane is also arranged between the cathode chamber and the protein production chamber; an anode is arranged in the anode chamber and connected with the positive pole of the power supply through a wire, and a resistor is arranged on the wire connecting the anode and the positive pole of the power supply. The microbial electrochemical system and the protein production system are coupled to form an ammonia-nitrogen recovery-in-situ protein synthesis microbial electrochemical system, the system can convert active nitrogen into high-value protein products in-situ, the system can synchronously treat wastewater, recover ammonia-nitrogen and fix carbon dioxide, and has excellent environmental benefits.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and / or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.

The invention discloses a domestication method for efficiently degrading tobacco waste to produce hydrogen by using mixed flora, which comprises the following steps: carrying out anaerobic enrichment domestication culture by taking manure compost as a bacterial source and cellulose prepared from tobacco waste as a core component of a domestication culture medium; the domestication culture medium contains cellulose, ammonium bicarbonate and monopotassium phosphate, and the cellulose concentration gradient is gradually increased without adjusting the pH value; stopping culturing when the hydrogen content in the biogas is reduced by 10%, transferring the biogas to a fresh domestication culture medium for passage according to an inoculum size of 10-20%, and continuously domesticating for 20-40 rounds; inoculating the domesticated mixed flora culture solution into a cellulose liquid fermentation culture medium containing microcrystalline cellulose, ammonium bicarbonate, monopotassium phosphate and a nutrient solution according to the inoculum size of 10-20%; the initial pH of the fermentation liquor is adjusted to 6.5-7.5, high-purity nitrogen is introduced to create an anaerobic environment, and hydrogen is produced through fermentation under the conditions of 35 + / -1 DEG C and 120 r / min. According to the method, flora is easy to culture, hydrogen production is efficient and stable, tobacco waste resources can be utilized, flora stability is high, adaptability is high, and strict sterile conditions are not needed.

The invention belongs to the technical field of microorganisms, and particularly discloses high-activity lactic acid bacteria powder as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out activation culture on lactic acid bacteria in a liquid culture medium to obtain an activated bacteria solution; adjusting the concentration of the activated bacterial liquid, standing and incubating, and in the incubation process, dynamically measuring the formation amount of a biological membrane by adopting a crystal violet staining method, and determining the optimal biological membrane incubation time of the lactic acid bacteria strain; adjusting the concentration of the activated bacterial liquid, standing and incubating to the determined optimal biological membrane incubation time; washing off thalli which are not adhered to the upper layer by using the sterile PBS, and centrifugally collecting biofilm state lactic acid bacteria which are adhered to the bottom of the culture container; and re-suspending the collected biofilm state lactic acid bacteria, and drying to obtain the high-activity lactic acid bacteria powder. According to the preparation method, the drying survival rate of the strain is remarkably improved, and the resistance of lactic acid bacteria to stress such as dehydration, heat stress and cold stress in the drying process is effectively enhanced.

The present invention relates to a method for hydrolyzing carbohydrates present in raw materials containing carbohydrates, comprising the step a) of contacting the raw material containing carbohydrate with a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a method for simultaneously liquifying and saccharifying starch, a method for simultaneously liquifying, saccharifying and fermenting starch, and to a method for producing a biomass, the method comprising the step of cultivating bacteria secreting carbohydrate-hydrolyzing enzymes on a medium comprising complex carbohydrates as a major carbon source.

The present disclosure relates to: a method for preparing heat-killed lactic acid bacteria.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The invention relates to a Saccharomyces cerevisiae C16I09 strain which is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the Saccharomyces cerevisiae C16I09 strain is CCTCC (China Center for Type Culture Collection) No. M20251150. The strain disclosed by the invention has good oxidation resistance and can be applied to the fields of skin care products, health care products, anti-aging foods and the like. Especially, extracellular fermentation broth of the strain, namely a fermentation product or a fermentation extract of the strain, has excellent oxidation resistance, can scavenge free radicals and relieve oxidative damage, has the effects of protecting skin, resisting inflammation, delaying senescence and the like, and can be applied to the fields of skin care products, health care products, anti-aging foods and the like as a natural anti-aging agent.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

The invention discloses a film-embedded conjugated oligomeric electrolyte based on a natural product, and the structure of the film-embedded conjugated oligomeric electrolyte takes a natural polyphenol compound as a conjugated skeleton part, so that the overall biocompatibility of molecules is improved, and the toxicity is reduced; alkyl chains with different lengths or numbers are used as side chain parts to adjust the compatibility with a cell membrane and promote the membrane embedding and retention of molecules, and the alkyl chains can disturb the phospholipid arrangement of the cell membrane and increase the membrane permeability after the molecules are embedded into the membrane; a positively charged group is used as an end group part, and a negatively charged cell membrane is anchored through electrostatic attraction, so that the local molecular concentration is improved. The cell membrane is modified by the embedded membrane conjugated oligomeric electrolyte derived from the natural product, so that the permeability of the cell membrane is improved, the cytotoxicity is reduced, and the catalytic efficiency of cells is improved. The cell membrane is modified by the embedded membrane conjugated oligomeric electrolyte derived from the natural product, so that the permeability of the cell membrane is improved, the cytotoxicity is reduced, and the catalytic efficiency of cells is improved.

The application discloses a heavy metal resistant bacillus velezensis and a microbial repair agent and application thereof. The bacillus velezensis (Bacillus velezensis DBS01) is screened from heavy metal contaminated soil in Dabaoshan mine area of Shaoguan city in Guangdong province, and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 20252228. The bacillus velezensis not only has multiple heavy metal resistance capabilities, but also can metabolically secrete organic acids, cellulase, growth hormones and other metabolites in different growth stages, so as to realize multiple functions such as heavy metal solidification, phosphorus dissolution, potassium dissolution and plant growth promotion. The bacillus velezensis DBS01 is inoculated into a culture medium containing straws, calcium superphosphate is added, and agar and sodium alginate are added, and the mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the mine heavy metal contaminated soil can be repaired in a high-efficiency, low-cost and environment-friendly manner, and the ecological function recovery of the soil can be effectively promoted.

This invention discloses a method for preparing a carbon source for microbial culture medium based on enzymatic hydrolysis of bamboo powder and its application, belonging to the field of high-value utilization technology of biomass resources. Using bamboo powder as raw material, the invention involves mild alkali pretreatment to disrupt the dense structure of lignocellulose, followed by enzymatic hydrolysis using a specialized enzyme system of endoglucanase Cel7B and xylanase XynA expressed heterologously by *E. coli* in an optimized ratio. After solid-liquid separation, an enzymatic hydrolysate rich in reducing sugars is obtained. The hydrolysate is then nutritionally fortified with trace elements such as sodium thiosulfate and sodium silicate to produce a low-cost, high-efficiency liquid carbon source. This invention achieves high-value utilization of bamboo waste. The entire process is mild, environmentally friendly, and simple, easily scaled up industrially, and solves the industry bottleneck of high carbon source cost in industrial microbial culture, demonstrating good application prospects and market value.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention relates to anti-CRISPR genes and anti-CRISPR proteins, and their uses in various biotechnology applications.

In alternative embodiments, the invention provides vaccines, pharmaceutical compounds and formulations for diagnosing, preventing, treating or ameliorating Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections. In alternative embodiments, the invention provides compositions such as diagnostic tests, assays, immunoassays and test strips, and methods, for detecting or diagnosing the presence of a Streptococcal infection, e.g., Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections.