916results about "Microbiology processes" patented technology

The invention belongs to the technical field of food microorganisms, and relates to lactobacillus plantarum and application thereof in preparation of products for relieving insomnia. The preservation number of the lactobacillus plantarum is GDMCC No: 65061. The lactobacillus plantarum can be applied to efficient fermentation of spina date seed juice, the content of GABA, polypeptide, total phenols and total flavonoids in the spina date seed juice is increased, and the content of spinosin, 6 ''-feruloyl spinosin, jujuboside A, jujuboside B and betulinic acid is increased; the reduction of whole brain coefficient and the increase of liver coefficient caused by long-term insomnia are relieved, the bad condition caused by long-term insomnia in a hippocampus DG region in the brain of the mouse is improved, the sleep time of the mouse is prolonged, and the insomnia symptom of the mouse is relieved; the content of inhibitory neurotransmitters 5-hydroxytryptamine, melatonin and gamma-aminobutyric acid in the hypothalamus of the mouse is increased, the content of excitatory neurotransmitter glutamic acid is reduced, and the reduction of organism immunity and oxidative stress of brain tissues caused by insomnia are relieved.

The invention relates to talaromyces purpurogenus D2 and application thereof, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D2 disclosed by the invention has the preservation number of GDMCC (Graphics Decific Microorganism Center) NO.66320 The strain is obtained by being separated from soil and domesticated, the zinc tolerance concentration of the strain can reach 2g / L, and the strain can efficiently convert inorganic zinc into organic active zinc which can be easily absorbed and utilized by a human body. The organic zinc prepared from the strain has a remarkable ABTS free radical scavenging capability, and also has tyrosine kinase and 5-alpha reductase inhibition capability; the organic zinc prepared from the talaromyces purpurogenum D2 can be used for preparing zinc-supplementing health-care products, can also be used as cosmetic active matter raw materials with free radical scavenging and whitening effects, and has a wide market application prospect.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention relates to bacillus pumilus and application thereof. The bacillus pumilus DX-fK is obtained through a chemical mutagenesis method, the bacillus pumilus DX-fK can efficiently degrade straw, the bacillus pumilus DX-fK is used as a substrate to produce feed alternative protein, and therefore the bacillus pumilus DX-fK can be applied industrially and has good industrialization prospects.

The invention belongs to the technical field of in-vitro cultivation of calculus bovis, and particularly relates to a method for in-vitro cultivation of calculus bovis. According to the invention, firstly, the escherichia coli is subjected to bile domestication and ray induced mutation, a high-activity oxgall-resistant bezoar production engineering bacterium E.coli-NH01 is obtained through screening, a method for in-vitro cultivation of bezoar by using the engineering bacterium E.coli-NH01 is provided, the prepared bezoar meets the pharmacopoeia standard, the production efficiency of in-vitro cultivation of bezoar is improved, and the production cost is reduced. And the production cost is reduced, the overall forming period is shortened, and the key that precious traditional Chinese medicinal materials are popular can be achieved.

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

The invention discloses a phosphate solubilizing bacterium with eurytopic characteristic and application thereof, the bacterium is separated from corn straw biochar in which rice is continuously planted for 3 years, is identified as Pseudomonas paaversuta and is named as MPP2401, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuchang District, Wuhan, Hubei province, the preservation date is November 6, 2024, and the preservation number is CCTCC NO: M20242439. The strain can keep stable phosphate solubilizing capacity under the environmental conditions of 15-30 DEG C, pH of 7-10 and NaCl 0-0.8 mol.L <-1 >, and still has functional phosphate solubilizing capacity even under the condition of extreme low temperature of 5 DEG C, the composite stress of pH of 7-8 and NaCl 0-0.8 mol.L <-1 > and the composite stress of pH of 9-10 and NaCl 0-0.6 mol.L <-1 >. Meanwhile, the bacterium has the broad-spectrum antibacterial characteristic, and the antibacterial rate on fusarium graminearum, fusarium verticillioides and magnaporthe oryzae reaches 41.0-77.6%. Due to the unique wide environment adaptability and the phosphate solubilizing-bacteriostasis dual-function synergistic characteristic, a microbial germplasm resource with production-ecology dual values is provided for biochar bacterial fertilizer development, saline-alkali phosphorus activation and biological prevention and control.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention discloses a saline-alkaline tolerant compound microbial agent and application thereof, and particularly relates to the technical field of microbial soil treatment. The microbial agent comprises Klebsiella, Enterobacter hormaechei and humic acid, the ratio of the components is as follows: Klebsiella: Enterobacter hormaechei: humic acid = 1: (0.5-2): (1-3), and both Klebsiella and Enterobacter hormaechei are obtained by fermentation culture. According to the present invention, the specific pH gradient is adopted for the Klebsiella and the Enterobacter hormaechei, and the segmented fermentation process combining the specific pH gradient with the corresponding gradually-increased sodium chloride concentration is adopted, such that the strain is effectively subjected to the directional domestication so as to maintain the activity and the function in the high-salt and high-alkali extreme environment.

The invention relates to bacillus velezensis LYB-S and application thereof in improving the reduction rate of hexavalent chromium in combination with exosomes, and belongs to the technical field of environmental restoration. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC NO.34429. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC). The bacillus velezensis LYB-S can be combined with an exosome, so that the reduction rate of the hexavalent chromium can be increased together with the bacillus velezensis LYB-S.

The invention discloses a streptococcus suis type 2 gene deletion strain as well as a construction method and application thereof, and belongs to the technical field of biological products. According to the invention, the B9H0105210 gene in the genome of the streptococcus suis serotype 2 SC19 is deleted in a homologous recombination manner for the first time, the hydrogen peroxide and nitric oxide stress resistance of the constructed streptococcus suis serotype 2 gene deleted strain is reduced, and the pathogenicity to mice is obviously weakened; moreover, the growth performance of the streptococcus suis type 2 gene-deleted strain is not greatly different from that of a wild strain SC19, the condition of gene recovery does not exist after continuous passage, and the streptococcus suis type 2 gene-deleted strain has an application prospect as an attenuated live vaccine, has a good immune protection effect on different genotypes of streptococcus suis such as the streptococcus suis type 2 strain SC19 and streptococcus suis type 2 P1 / 7, and has a good application prospect. And a certain cross immune protection performance is achieved.

The invention provides N-Fe co-doped biochar, a preparation method and application of the N-Fe co-doped biochar in production of medium-chain carboxylic acid by anaerobic digestion. The preparation method comprises the following steps: S1, pretreating a nitrogen-rich biomass raw material; s2, pyrolyzing and carbonizing the pretreated nitrogen-rich biomass raw material to obtain N-doped biochar; s3, adding FeCl3. 6H2O, a stabilizer, a buffer agent and N-doped biochar into an organic solvent, and uniformly mixing to obtain a mixed solution; s4, performing microwave-assisted solvent heat treatment on the mixed solution to obtain a precursor mixed solution; and S5, carrying out centrifugal washing on the precursor mixed solution, filtering, and drying to obtain the N-Fe co-doped biochar. On one hand, the required nitrogen element is obtained by using the nitrogen-rich biomass raw material, and the use of an exogenous nitrogen-containing material is avoided; on the other hand, the biochar is kept in a good dispersion state in a solution through microwave solvent heat treatment, agglomeration of particles is avoided, the electrical conductivity, the thermal conductivity and the mechanical strength of the biochar are improved, and the N-Fe co-doped biochar can also be used for producing medium-chain carboxylic acid through anaerobic digestion.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

A functional microbial organic fertilizer for improving saline-alkali land, and its preparation method and application, belong to the field of fertilizer technology. In order to solve the technical problem that the high salt ion content in saline-alkali soil has multiple adverse effects on plant growth, the present invention provides a functional microbial organic fertilizer for improving saline-alkali land, which is prepared by a functional microbial agent prepared from Bacillus amyloliquefaciens, Copperbacterium insecticide, Trichoderma harzianum, Halobacterium gesneri and Bacillus halogenide, and a functional microbial carrier organic fertilizer added with furfural residue and citric acid residue. The functional microbial organic fertilizer provided has the functions of increasing the abundance of saline-alkali soil microorganisms, improving soil physical and chemical properties, regulating soil pH, increasing the proportion of soil aggregates, and increasing the content of available phosphorus and available potassium. The functional microbial organic fertilizer provided by the present invention can be used to effectively improve saline-alkali soil.

There is provided an interleukin-21 (IL-21) variant, wherein in comparison to wild-type IL-21 that comprises an amino acid sequence according to SEQ ID NO: 6 or an amino acid sequence comprising at least 70% sequence identity to SEQ ID NO: 6, the IL-21 variant comprises an amino acid modification at one or more of the following amino acid positions: 29, 33, 36 and / or 71.

The invention provides a method for sorting chlorinated organic matter degrading bacteria, and belongs to the technical field of organic matter degrading bacteria sorting.The sorting method comprises the following steps that 1, microorganisms are separated from an environment sample polluted by chlorinated organic matter, and a microbial community is obtained; (2) domesticating the microbial community obtained in the step (1) by using a chlorinated organic matter until the degradation effect of the microbial community on the chlorinated organic matter is stable, so as to obtain a domesticated flora; 3) inoculating the domesticated flora into a heavy water culture medium for culturing to obtain a heavy water labeled flora; and 4) sorting the heavy water labeled flora under the condition of chlorinated organic matter pollution stress by using a single-cell Raman sorting platform, and screening cells with a heavy water labeled characteristic peak and a VB12 characteristic peak as target cells, namely the chlorinated organic matter degrading bacteria. Based on the single-cell Raman sorting technology and in combination with the anaerobic culture technology, the sorting efficiency of the anaerobic chlorinated organic matter degrading bacteria is remarkably improved.

The present invention relates to recombinant virulence attenuated Gram-negative bacterial strains and its use in a method of treating cancer in a subject.

The invention provides a method for realizing high yield of bile salt hydrolase by probiotics by utilizing intestinal selective pressure and application, and the method comprises the following steps: changing the intestinal selective pressure by high fat diet to promote adaptive evolution of bifidobacterium animalis H22B656 to obtain a mutant strain for efficiently producing bile salt hydrolase. Directional breeding is carried out through a sterile mouse model and intestinal tract selection pressure, the mutant strain with the bile salt hydrolase production capacity and the functional effect of the probiotics remarkably enhanced is screened out, and a new strategy is provided for functional probiotic development and accurate micro-ecological intervention of metabolic diseases.

The invention aims to provide a morchella esculenta breeding method, an obtained morchella esculenta strain and application, the morchella esculenta is named as Morchella sextella NYD1, the taxonomic name of the morchella esculenta is Morchella sextella NYD1, the morchella esculenta is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the morchella esculenta is GDMCC No: 66082, and the preservation date of the morchella esculenta is April 21, 2025. The morchella strain is further utilized as a mother strain to carry out domestication and cultivation, the morchella with the fruiting yield up to 400 kg / mu or above, excellent agronomic characters and rich nutritional ingredients such as crude protein, total sugar, iron, zinc and calcium is finally obtained, the content of the crude protein is not lower than 23 g / 100 g, the content of the total sugar is not lower than 55 g / 100 g, the content of the iron is not lower than 240 mg / kg, the content of the zinc is not lower than 70 mg / kg, and the content of the calcium is not lower than 76 mg / kg.

The invention belongs to the field of microbial agents, and particularly relates to a microbial composite agent for degrading herbicides and a preparation method of the microbial composite agent. The preparation method of the microbial complex microbial inoculant comprises the following preparation steps: mixing bran hydrolysate, peat soil and diatomite, sterilizing, adding a protective agent and a complex flora suspension in a sterile environment, uniformly mixing, kneading and forming, preparing into slices, naturally air-drying, and crushing to obtain the microbial inoculant, and uniformly mixing the microbial agent with the adsorbent, the surfactant and the nutrient substances to obtain the microbial complex microbial inoculant. According to the invention, domesticated microorganisms form a composite flora to prepare the microbial agent, and the composite flora exerts unique degradation capability and generates synergistic effect, so that the herbicide can be degraded more comprehensively and efficiently, and the proportion of each microorganism, an adsorbent, a surfactant and other components in the microbial agent can be adjusted according to different herbicide types and polluted environments; and efficient degradation of specific herbicides is realized.

The invention relates to a method and system for in-situ treatment of fluorine-containing mine water in cooperation with a coal mine underground reservoir. The method comprises the following steps: S1, screening coal gangue of which the coal content is 5-20% and the particle size is less than or equal to 5mm; s2, fluorine-containing mine water or sediment collected near the site to be treated is used as a sample, the sample is subjected to screening, enrichment and domestication of mixed anaerobic flora, and efficient defluorination mixed bacteria are obtained; s3, the bacterial liquid of the efficient defluorination mixed bacteria and the coal gangue are subjected to mixed culture, and microbial biofilm culturing coal gangue is obtained; and S4, adding the microbial biofilm formation coal gangue into a material adding mechanism, and adding the microbial biofilm formation coal gangue into mine water of a coal mine underground reservoir through the material adding mechanism for defluorination treatment. According to the invention, in-situ defluorination can be carried out on the mine water of the coal mine underground reservoir, and the purification treatment effect of the coal mine underground reservoir on the mine water is greatly improved.

The invention discloses a bisphenol A degrading bacterium XYQ100 as well as a culture method and application thereof, and relates to the technical field of microbial pollution degradation and environmental pollution, the bisphenol A degrading bacterium XYQ100 provided by the invention is classified and named as sphingobacterium sp., and is preserved in the China General Microbiological Culture Collection Center on April 18, 2025, and the preservation number of the strain is CGMCC NO.34264. The bisphenol A degrading bacterium XYQ100 provided by the invention is named as sphingobacterium sp. The strain can realize efficient removal of bisphenol A pollutants, and the degradation rate of bisphenol A within 54 hours reaches 100%.

The invention discloses a multifunctional composite microbial inoculant for improving drought resistance of plants as well as a preparation method and application of the multifunctional composite microbial inoculant, and belongs to the field of microbiology. According to the multifunctional composite microbial inoculant, by establishing a culture medium with gradually increased PEG (Polyethylene Glycol) concentration, progressive drought resistance induction is carried out on a known functional strain, so that the strain damage caused by single high-intensity stress is effectively avoided, and the strain can still keep relatively high activity and stability under a drought condition; progressive drought-resistant induction enables the strain to gradually adapt to the drought environment, so that the drought resistance of the strain is enhanced, the survival rate of the strain under the drought condition is increased, and the strain can better play a role in drought resistance and growth promotion; the multifunctional composite microbial inoculant is applied to plant seedling root system field planting, stress resistance phenotype induction and ecological adaptability improvement in arid / semi-arid regions, the colonization survival rate of seedling rhizosphere of caragana microphylla is increased to 2.3 times of that of conventional treatment, the transplanting survival rate of caragana microphylla is increased to 78% from 42%, and the multifunctional composite microbial inoculant has wide application prospects.

The invention provides a method for preparing rare ginsenoside through conversion of kombucha, the rare ginsenoside and application, and belongs to the technical field of biological medicine. A symbiotic system is formed by multiple bacteria such as acetic acid bacteria, gluconacetobacter, foal bacillus and the like and fungi, after the symbiotic system is domesticated, the American ginseng stem and leaf extract is fermented through multi-bacteria synergistic catalysis, the rare ginsenoside can be efficiently prepared, the highest conversion rate of Rb3 and Rd can reach about 90%, and the yield of Rb3 and Rd can reach about 90%. Meanwhile, the contents of rare ginsenosides Rg3 (20S), Rg5, Rh2 (20R) and Rh2 (20S) are remarkably increased and are increased by about 15 times at most, and efficient conversion of various ginsenosides is achieved.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The present invention relates to a method for producing a mycelium sheet. The invention discloses a method for producing a mycelium sheet, which comprises the following steps of: standing and culturing fungi in a liquid culture medium containing cellulose, so that the mycelium sheet is formed on the surface of the liquid culture medium; and collecting the mycelium sheet from the surface of the liquid culture medium containing cellulose.