291 results about "Bacillus sp. BI" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention discloses a biocontrol bacterium composition and application thereof in prevention and treatment of corn diseases, and relates to the technical field of biology. The biocontrol bacterium composition comprises bacillus velezensis (Bacillus velezensis) HPP-0-TS7 and bacillus subtilis (Bacillus subtilis) HPP-0-GZL7, and the biocontrol bacterium composition comprises bacillus velezensis (Bacillus velezensis) HPP-0-TS7 and bacillus subtilis (Bacillus subtilis) HPP-0-GZL7. The biocontrol bacterium composition provided by the invention shows excellent technical effects in the aspects of preventing and treating corn diseases and promoting plant growth, and the two strains have a synergistic effect, so that not only is the prevention and control capability on specific diseases enhanced, but also the antibacterial spectrum is expanded, and a reliable guarantee is provided for comprehensive prevention and treatment; a good interaction relationship exists between the two strains, and the two strains jointly play a role through different action mechanisms, so that the stress resistance and yield potential of corn are comprehensively improved.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention provides bacillus velezensis H5 capable of producing broad-spectrum antibacterial substances and application of the bacillus velezensis H5. The preservation number of the bacillus velezensis H5 in the China General Microbiological Culture Collection Center is CGMCC No.32671. The bacillus velezensis H5 is separated from sea mud in a specific sea area, can generate antibacterial substances with broad-spectrum antibacterial activity, and mainly comprises bacitracin, surfactin and mycostatin. The antibacterial substance of the bacillus velezensis H5 is applied to preservation of perishable fruits such as grapes and the like and mildew prevention of cereals such as corn and the like, and can effectively inhibit spoilage of the perishable fruits and synthesis of mycotoxins such as aflatoxin and the like, and the action effect of the antibacterial substance is obviously superior to that of a food preservative natamycin. The invention provides a novel, green, safe and broad-spectrum antibacterial substance for preservation and fresh-keeping of fruits and mildew prevention of grains, and has important application value.

A microorganism according to the present disclosure is a microorganism (accession number: NITE BP-03832) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04073) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04126) belonging to Bacillus velezensis, or a variant of these having a plant disease prevention effect.

The invention discloses a method for inhibiting production of vomitoxin from bacillus cereus, which is characterized in that isoleucine is added into a substance needing to inhibit production of vomitoxin from bacillus cereus, preferably 5-20mM of isoleucine is added. According to the present invention, the content of the toxin is detected through the liquid chromatography-mass spectrometry, and the cytotoxicity experiment verification results show that the isoleucine can significantly inhibit the synthesis of the vomitoxin of the bacillus cereus in the culture medium and the matrix, and can effectively reduce the toxic effect of the toxin on HeLa cells; the invention provides a safe and efficient biological control means which is suitable for various food systems such as starch food, dairy products, meat products and the like. Isoleucine can be used as a novel non-toxic and harmless food additive and is low in cost; the use method is simple, the output of vomitoxin can be reduced by directly adding a trace amount of isoleucine, and the food flavor is not affected.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis NEAU-L05, application of the bacillus velezensis NEAU-L05, antibacterial cyclic lipopeptide Iturin A and application of the antibacterial cyclic lipopeptide Iturin A. The bacillus velezensis NEAU-L05 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 19, 2024, and the preservation number is CGMCC No.33130. The bacillus velezensis NEAU-L05 disclosed by the invention can be used for preparing the bacillus velezensis NEAU-L05. The bacteriostasis rate of the bacterial strain to botrytis cinerea of phytopathogen is 67.2%. The bacteriostatic cyclic lipopeptide ItuinA separated from the strain can improve the storage performance of berries, and a basis is provided for development of multiple biocontrol microbial agents with excellent performance.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus velezensis DZ-4-002 and application thereof. According to the bacillus velezensis DZ-4-002 provided by the invention, the Latin name is bacillus velezensis, and the preservation number of the bacillus velezensis DZ-4-002 is CGMCC (China General Microbiological Culture Collection Center) No.25955. The strain not only can produce nitrite reductase at high yield, but also can improve the growth performance of animals and the milk production performance of cows. Within the experimental range of the invention, the bacillus velezensis provided by the invention not only can improve the egg production increase rate, the average egg weight and the feed utilization rate of laying hens, but also can improve the average daily gain, the average daily feed intake and the feed utilization rate of weaned piglets, and the average milk yield, the milk-feed ratio and the lactose content in milk of dairy cows. The method has a good application prospect in the fields of nitrite degradation agents, feeds, additives of the feeds and the like.

The invention belongs to the field of microbial preparations, and discloses a microbial stabilizer which comprises non-bacillus or a zymophyte solution thereof and a chemical stabilizer, and the non-bacillus comprises at least one of serratia nematophila and acinetobacter calcoaceticus. The invention also discloses a preparation method of the microbial stabilizer and an application of the microbial stabilizer in prolonging the shelf life of a microbial agent. The research on the microbial stabilizer containing non-bacillus discovers that the microbial stabilizer formed by proper matching of the non-bacillus and the chemical stabilizer can keep the stability of the concentration of viable bacteria for a long time, prolong the shelf life of microbial products and reduce the requirements of frequent production and storage; therefore, the production cost is directly reduced, the economic benefit is improved, and the method can be used in the technical field of microbial preparations.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The present application relates to a novel Bacillus promoter variant and a protein production method using same. When the novel Bacillus promoter variant of the present application is used, the expression of a target protein can be significantly increased in a host cell, and thus the novel Bacillus promoter variant can be effectively used in the production of biotechnologically, medically, and industrially useful proteins through the mass production of proteins and target proteins such as polypeptide drugs.

The invention discloses a novel bacillus, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 1.62901, and the preservation date of which is September 20, 2024. Gene identification shows that the strain belongs to a new microbial species in taxonomy. Researches show that the strain has nitrogen fixation capacity, can grow in a Mengjinna inorganic phosphorus culture medium, exerts phosphorus dissolving capacity, converts insoluble phosphorus elements which are difficult to absorb and utilize by organisms into dissolved phosphate ions which can be efficiently absorbed and utilized by plants, and provides a large number of nutrient substances for crop production; in addition, the strain has good endurance capacity to low-temperature and high-salt environments, and can play an environment restoration function in the low-temperature environments or saline-alkali areas. Therefore, the novel bacillus disclosed by the invention has better application potential and prospect in the aspects of agricultural production (microbial phosphate solubilization) and environmental restoration.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention relates to the technical field of microbial technology and rare earth element recovery. The invention provides bacillus tequilensis Re2, and the preservation number of the bacillus tequilensis Re2 is CGMCC (China General Microbiological Culture Collection Center) No. 36764. The strain Re2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 25, 2025, the center is called CGMCC for short, the address of the center is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number of the strain Re2 is CGMCC No.36764, and the strain Re2 is classified and named as Bacillus tequilensis. The bacillus tequilensis provided by the invention can efficiently adsorb various rare earth elements, and the rare earth elements in spores can be eluted after 2, 6-dipicolinic acid (DPA) is added; therefore, the repeated utilization of the strain is realized.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention relates to the technical field of phycocyanobilin, and particularly discloses a method for producing phycocyanobilin by modifying bacillus subtilis, which comprises the following steps: preparation of a culture medium, synthesis of phycocyanobilin biosynthesis key genes, construction of a carrier, and construction of phycocyanobilin synthesis efficient coenzyme factor circulation. According to the method, a ferredoxin oxidoreductase gene PcyA is used as a template, an ancestral sequence of the ferredoxin oxidoreductase gene PcyA is synthesized, a bacillus subtilis expression system is used for expressing a heterologous synthesized phycocyanobilin gene, and the yield of phycocyanobilin is increased by increasing synthesis of coenzyme and reaches 20.42 mg / L in a shake flask; the yield of the phycocyanobilin in the bacillus expression system is increased, the use of the bacillus expression system simplifies the step of removing endotoxin for the subsequent purification of the phycocyanobilin, and the purification and use of the phycocyanobilin are safer.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention discloses bacillus velezensis, a microbial preparation as well as a preparation method and application of the microbial preparation, and relates to the technical field of microorganisms. The bacillus velezensis strain is preserved in the China Center for Type Culture Collection, and is named as bacillus velezensis XNW-BLSYB1, the preservation number is CCTCC NO: M 2025721, the preservation time is April 8, 2025, and the 16S rDNA sequence of the bacillus velezensis strain is as shown in SEQID No.1. The bacillus velezensis strain has the advantages that the bacillus velezensis strain is named as bacillus velezensis XNW-BLSYB1; the spore formed by the bacillus velezensis XNW-BLSYB1 strain can survive at high temperature, and can inhibit the reproductive and respiratory syndrome virus and inhibit the activity of bacteria such as streptococcus suis, infectious actinobacillus pleuropneumoniae, clostridium perfringens, pasteurella multocida and escherichia coli.

The invention discloses bacillus subtilis Bs1 as well as an antagonistic substance and application thereof. According to the invention, Bs1 with antagonism on colletotrichum gloeosporioides is separated and screened for the first time, an antagonistic substance is clear as Bs1 serine-producing protease Vpr, a Bs1 bacteriostasis mechanism and biological control potential excavation are analyzed in a multi-omics manner, a peripheral colonization rule of Bs1-Rif is clear, and an indoor control strategy of the strain Bs1 on cunninghamia lanceolata anthracnose is optimized. When the strain Bs1 is applied on the day when pathogenic bacteria are inoculated, a certain prevention and treatment effect is achieved, and it is indicated that the strain Bs1 can rapidly occupy the ecological niche; the average antibacterial rate of the strain Bs1 can reach 66.69%. The biocontrol strain and theoretical basis are provided for prevention and control of Chinese fir diseases, the application of biological prevention and control in forestry is promoted, the interaction mechanism of biocontrol bacteria and pathogenic bacteria can be deeply understood, and the sustainable development requirement of modern forestry is met.

The invention relates to the technical field of microbial preparations, and particularly discloses bacillus velezensis HY16 fermentation liquor freeze-dried powder and a preparation method and application thereof.The preparation method includes the steps that hydroxyl of beta-cyclodextrin reacts with anhydride of maleic anhydride, double bonds are introduced to beta-cyclodextrin molecules, and then under the action of persulfate serving as an initiator, the beta-cyclodextrin and maleic anhydride react with each other to prepare beta-cyclodextrin HY16 fermentation liquor freeze-dried powder; and carrying out free radical copolymerization reaction on the beta-cyclodextrin and 2-acrylamide-2-methylpropanesulfonic acid to obtain the modified beta-cyclodextrin. Sulfonic acid groups in 2-acrylamide-2-methylpropanesulfonic acid are strong hydrophilic groups, the water solubility of beta-cyclodextrin can be remarkably improved, growth of ice crystals during freezing is delayed, mechanical damage of the ice crystals to strains is reduced, and meanwhile negative charges of the sulfonic acid groups can be effectively absorbed through electrostatic repulsion. The freeze-drying process is simple, aggregation and inactivation of fermentation active substances or strains in the freeze-drying process are prevented, the survival rate of viable bacteria in the freeze-drying powder is increased, the stability of active ingredients in fermentation liquor is kept, and then the algae-lysing effect is improved.

The invention relates to the field of microorganisms, and discloses a strain of bacillus aryabhattai, which is characterized in that the preservation number of the bacillus aryabhattai is CCTCC (China Center for Type Culture Collection) NO: M 2024282. The bacillus aryabhattai provided by the invention can inhibit the activity of the pathogenic bacterium fusarium oxysporum f.sp.cubense of banana wilt, so that the banana wilt can be prevented and treated, and in addition, the bacillus aryabhattai also has a remarkable growth promoting effect on banana plants.

The present invention belongs to the field of microbial technology and relates to a strain of Bacillus subtilis subsp. and its application in degrading lignin. It is named Bacillus subtilis subsp. NBL-B12058, deposited in the China Center for Type Culture Collection on October 17, 2022, with the deposit number CCTCC NO: M20221574, and the deposit address is Wuhan University, Wuhan, China. The Bacillus subtilis subsp. provided by the present invention has a good degradation effect on lignin in crop straws and the black water of paper mills.

The invention discloses bacillus cereus with wide pH adaptability and application of the bacillus cereus in soil or underground water heavy metal remediation, the strain is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20241939), and the bacillus cereus is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (CCTCC). The strain is high in environmental adaptability and high in remediation efficiency, can show good reduction and endurance capacity to various heavy metal pollutants under the wide environmental conditions that the pH ranges from 4 to 10 and the temperature ranges from 16 DEG C to 37 DEG C, and is particularly suitable for in-situ remediation of high-concentration contaminated soil; experimental results represented by hexavalent chromium show that the removal rate of the strain in 1000 mg / kg hexavalent chromium contaminated soil within 7 days can reach 70-100%, the dependence of an existing microbial remediation technology on a low-concentration and alkaline environment or a long remediation period is broken through, and an efficient solution is provided for green bioremediation of multiple types of heavy metal contaminated sites.

The invention relates to the technical field of genetic engineering, in particular to a bacillus subtilis engineering strain as well as a preparation method and application thereof. According to the invention, the bacillus subtilis WB800n is used as an original strain, and the IL-2 gene and the DnaK gene are introduced, so that heterologous expression of the IL-2 is realized, and the yield of the IL-2 is increased. Meanwhile, the IL-2-containing bacillus subtilis disclosed by the invention can be used for improving the immunity and egg laying performance of poultry, and has a good application effect.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

This invention discloses a compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis, its preparation method, and its application, belonging to the field of agricultural microbiology. The compound microbial agent comprises halophilic Bacillus and Bacillus belye, and an agriculturally acceptable carrier; wherein, single bacterial suspensions of halophilic Bacillus and Bacillus belye are mixed to obtain a compound bacterial suspension, and the compound bacterial suspension is further compounded with an agriculturally acceptable carrier to obtain the compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis. The halophilic Bacillus is classified and named... Bacillus halotolerans X14-2, classified as Bacillus belye Bacillus velezensis X147. This invention solves the problems of insufficient adaptability and poor growth-promoting effect caused by the single strain in the microbial agent in the prior art. It has the potential to prepare products that alleviate the continuous cropping obstacles of Scutellaria baicalensis or promote the growth of Scutellaria baicalensis.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.