645 results about "Bacillus sp. BI" patented technology

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and / or isobutyric acid (as part of the catabolism of valine and / or isoleucine), 3) butanol and / or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and / or 5) cadaverine and / or putrescine (as parts of the catabolism of lysine and / or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

Novel variant α-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.2) are deleted. The disclosed variant α-amylase enzymes show altered or improved stability and / or activity profiles.

The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

Belonging to the field of environmental microorganism application, the invention discloses a Bacillus subtilis strain and application thereof. The strain is named as Bacillus subtilis CM-A12, is currently preserved in China Center For Type Culture Collection (called CCTCC for short) with a preservation number of CCTCC NO:M 2015688, and the preservation date is November 23, 2015. The Bacillus subtilis strain provided by the invention has the advantages of simple cultivation condition, high temperature resistance, acid and alkali resistance, and high salinity resistance, can secrete protease, amylase and cellulase, and can effectively improve water quality and reduce COD and ammonia nitrogen in water.

A method of treating autism using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.

The invention relates to a Bacillus subtilis ANSB 060CGMCC No.3440 used for degrading aflatoxins, and culture method and application thereof. The invention also provides a method for degrading aflatoxins B1, G1 and M1 by fermentation liquor of the strain. By the method, the Bacillus subtilis is reacted with the aflatoxin B1 for 2 hours, has a degradation rate of over 70 percent on the aflatoxin B1, and has the degradation rate of over 80 percent after the reaction performed for 12 hours; and the Bacillus subtilis has the degradation rates of over 80 percent and over 60 percent on the aflatoxin G1 and aflatoxin M1 respectively. The Bacillus subtilis has high detoxification activity, strong specificity, mild action effect, and no damage to nutrient in feeds, and is suitable for removing the aflatoxins in the feeds.

Products and methods for controlling animal wastes are disclosed. The methods include making an inoculum containing a mixture of Bacillus species; mixing the inoculum with animal wastes and then incubating the mixture to result in an enriched culture; and then applying the enriched culture to the animal wastes. The inoculum for controlling wastes includes a mixture of Bacillus species in a liquid medium in which the mixture of Bacillus species were incubated.

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention provides bacillus velezensis as well as a separation screening method and application thereof, and belongs to the field of microbe technology and food processing. The invention providesthe bacillus velezensis with high fermentation capability; the bacillus velezensis is obtained through screening; during the fermentation, the capability of high yielding protease, amylase and fermented bean plasmin is realized; the bacillus velezensis can be applied to food fermentation in a large scale when being prepared into a food fermentation strain; fermentation food obtained through fermentation by using the bacillus velezensis, the contents of free amino acid and douchi fibrinolytic enzyme are high; the nutrition is rich; the characteristics of easy digestion, thrombus prevention andthe like are realized.

A probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders, such as irritable bowel syndrome, autism, and fibromyalgia.

The invention relates to Bacillus pumilus NMCC 46, which is preserved in CGMCC with preservation date as November 2, 2009, and preservation number as CGMCC No.3378. The Bacillus pumilus NMCC 46 CGMCC No.3378 in the invention is a biological control bacillus separated from grass root surrounding soil near Namco Lake in Tibet, which can grow at 10 DEG C, is favorable for exerting growth promotion and biological control effects, can restrain soybean root pythium myriotylum and saccharomycetes, can boost growth of Arabidopsis and soybean, has a prevention and control effects on soybean root pythium myriotylum as high as 85%, can increase yield of soybean to 24%, and has good application prospect.

The invention provides a kind of Brevibacillus laterosporus (STP-B.latCGMCCNo.1119), the character follows this. G+, haulm shape, the shape is (0.4-0.5)*(2-2.5)mu. The spore adnation is from neutrality to sub-neutrality. The spore bursa will enlarge. Most of the spore is vertebra aitch, the remainder is disciform. VP-, ph value of culture fluid is 7.2, amylolysis-, gelatin dispergation-, decomposition casein+, katalase+, oxidase-, cirate -, dextrose aerogenesis-, dextrose acidgenesis+, L-arabinose acidgenesis-, xylose acidgenesis-, mannit acidgenesis+, azotate deocidisation+, methyl red+, pH5.7 growth+.

The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

The invention relates to a straw decomposing inoculant which comprises the following components of 0.2-1.5 billion / g of bacillus subtilis, 0.2-1.5 billion / g of bacillus licheniformis, 20-150 million / g of thermophilic sporotrichosis, 20-150 million / g of Trichoderma Koningii and 20-150 million / g of Phanerochete chrysosporium. Therefore, the straw decomposing inoculant has the advantages of low cost and short decomposing time and is practical and easy to operate.

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

The invention discloses a microbial composite treatment for gold ore tailings and an application method and belongs to the field of road engineering. According to the method, Bacillus pasteurii is compounded with one or more of Bacillus subtilis, Bacillus pumilus or Bacillus megaterium, so that by means of the Bacillus pasteurii, CaCO3 crystal is deposited and generated in the gaps in the gold oretailings, thus improving mechanical performance and stability and immobilizing heavy metal and noble metal ions in the gold ore tailings. The Bacillus pumilus or Bacillus megaterium can decompose thecyanides in the gold ore tailings. Through mechanical ploughing and multi-round slurry injection, the microorganisms, the gold ore tailings and a cemented liquid are fully mixed and contacted with each other, so that purposes of generating the CaCO3 crystal for reinforcing the gold ore tailings and degrading the cyanides can be achieved. The method has no secondary pollution, is low-cost, is simple and is high in resource utilization rate, and can achieve multi-channel large-scale recovery utilization of the tailings.

A method of inducing systemic acquired resistance to infection in a plant by applying a composition comprising a Bacillus control agent to the foliage of said plant wherein said plant is capable of producing defense proteins.

The invention discloses recombinant bacillus subtitles capable of increasing the yield of acetylglucosamine and belongs to the field of genetic engineering. The recombinant bacillus subtitles is characterized in that bacillus subtitles BSGNKAP-Pxy1A-glmS-P43-GNA1 is used as the original strain, a phosphatase yqaB gene controlled by a strong constitutive promoter Pveg is integrated to the genome, and the promoter of 6-phosphate glucosamine synthase is replaced by the strong constitutive promoter Pveg to obtain genetically engineered bacillus subtitles BSGNY-Pveg-glmS-P43-GNA1 accumulating the acetylglucosamine. The recombinant bacillus subtitles has the advantages that the yield of the acetylglucosamine of a 3L fermentation tank reaches 60.5g / L, the yield of the acetylglucosamine is 0.311g / g glucose, production intensity is 0.983g / L / h, the yield of the acetylglucosamine outside the cells of the recombinant bacillus subtitles is increased, and a foundation is laid for glucosamine production using metabolic engineering modified bacillus subtitles.

The invention provides Bacillus velezensis with the preservation number of CGMCC No.19215. The Bacillus velezensis and fermentation liquor and metabolites thereof can be used for inhibiting phytopathogen and promoting plant growth. The Bacillus velezensis has the biocontrol and growth-promoting effects, can effectively reduce soil-borne diseases of crops such as tomatoes, strawberries and corn, and reduces the usage amount of chemical agents. A preparation prepared from the Bacillus velezensis is stable in storage performance and good in control effect, can be in direct contact with crop rootsystems, has no adverse effect on plant growth and development, and is convenient to process and low in production and transportation cost.

The invention discloses bacillus subtilis JYM841 with antibacterial activity. The bacillus subtilis is preserved in a China center for type culture collection in January 10, 2014, and the preservation number is CCTCC NO: M 2014012. The bacillus subtilis JYM841 has a strong inhibitory effect on staphylococcus aureus and candida albicans, is high in antibacterial activity, wide in antibacterial spectrum, high in enzyme production, sensitive to bacterial antibiotic, drug-resistant to fungal antibiotic, high in heat-resisting ability and tolerant to acid and alkaline, has high antibacterial activity after treated at the temperature of 100 DEG C for 20min, and has tolerance to various enzymes such as trypsin, papain and protease K. An acid and alkali resistant pH (potential of hydrogen) value ranges from 6 to 10, and metabolite is stable, so that the bacillus subtilis can be applied to preparation of medicines for treating genital tract infection.

Genetically modified microorganisms having the ability to produce D(−)-lactic acid at temperatures between 30° C. and 55° C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and / or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.

The invention discloses recombinant bacillus subtilis for accumulating acetylglucosamine and application thereof, and belongs to the field of genetic engineering. According to the recombinant bacillus subtilis for accumulating the acetylglucosamine and the application thereof, recombinant bacillus subtilis BSGN6-PxylA-glmS serves as an original strain, the glucosamine synthesizing route is enhanced by excessively expressing glucosamine histone acetyltransferase coding genes (CeGNA1) from caenorhabditis elegans, and the genetically engineered bacterium bacillus subtilis for accumulating the acetylglucosamine is obtained; the yield on the shake flask level reaches 7.31 g / L, and a foundation is laid for further producing the glucosamine by transforming the bacillus subtilis in metabolic engineering.