48 results about "Bacillus sp. BI" patented technology

This invention discloses a compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis, its preparation method, and its application, belonging to the field of agricultural microbiology. The compound microbial agent comprises halophilic Bacillus and Bacillus belye, and an agriculturally acceptable carrier; wherein, single bacterial suspensions of halophilic Bacillus and Bacillus belye are mixed to obtain a compound bacterial suspension, and the compound bacterial suspension is further compounded with an agriculturally acceptable carrier to obtain the compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis. The halophilic Bacillus is classified and named... Bacillus halotolerans X14-2, classified as Bacillus belye Bacillus velezensis X147. This invention solves the problems of insufficient adaptability and poor growth-promoting effect caused by the single strain in the microbial agent in the prior art. It has the potential to prepare products that alleviate the continuous cropping obstacles of Scutellaria baicalensis or promote the growth of Scutellaria baicalensis.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The present application belongs to the field of microbial preparation, and discloses a kind of microbial stabilizer, including non-bacillus or its fermentation bacteria liquid, chemical stabilizer, wherein, the non-bacillus includes at least one of Serratia entomophila, Acinetobacter calcoaceticus.The present application also discloses the preparation method of the microbial stabilizer and its application in prolonging the shelf life of microbial agent.The present application studies the microbial stabilizer containing non-bacillus, finds that the microbial stabilizer formed by the suitable collocation of non-bacillus and chemical stabilizer can maintain the stability of viable bacterial concentration for a long time, can prolong the shelf life of microbial product, reduces the need for frequent production and storage, can ensure the supply of product in market more stable, thereby directly reduces the production cost, improves economic benefit, can be used in the field of microbial preparation technology.

The present disclosure is generally related to recombinant microbial cells expressing heterologous proteins of interest. Certain aspects of the disclosure are therefore related to, inter alia, recombinant Bacillus cells having enhanced protein production capabilities, novel protein signal sequences, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and / or methods thereof. Thus, as exemplified herein, the recombinant Bacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.

The application discloses a bacillus zoogleoides BZ-32 and application of metabolites of the bacillus zoogleoides BZ-32 in vegetable stress resistance, and an effective component of the application is composed of bacillus zoogleoides fermentation liquor or metabolites, choline chloride and lentinan, wherein the weight percentage of the bacillus zoogleoides, choline chloride and lentinan is respectively 5-30% of bacillus zoogleoides metabolites, 5-30% of choline chloride and 0.2-5.0% of lentinan. The plant stress resistance composition can prevent and treat diseases such as root rot, anthracnose and powdery mildew of vegetables, and can prevent stress caused by excessively low temperature in winter and spring frost, and improve disease resistance and stress resistance of crops. The application can also be used for preventing and treating various corn diseases and strawberry diseases, and promoting growth and improving stress resistance.

This invention provides a method for mineralizing and recovering dysprosium ions from Bacillus pasteurellii, comprising the following steps: 1) Cell culture: Activated Bacillus pasteurellii is inoculated into CASO liquid medium and cultured for 22-26 hours, then centrifuged to collect the cell bodies; 2) Induced mineralization of Bacillus pasteurellii: The cell bodies collected in step 1) are mixed with a calcium source, urea, and dysprosium ions (Dysprosium). 3+ The dysprosium-containing precipitate was obtained by incubation with an inducing solution and filtration. This invention belongs to the field of bio-environmental protection technology. The method for mineralizing and recovering dysprosium ions using Bacillus pasteurellium provided by this invention can efficiently mineralize rare earth ions Dysprosium. 3+ No need to deal with Dy 3+ After pretreatment of the solution and biomineralization, the dysprosium-containing precipitate can be directly obtained by filtration without centrifugation, making the operation simple. 3+ The recovery rate is as high as 98.4%.

This invention belongs to the field of microbial technology, specifically relating to a type of endophytic Bacillus belye AN6 in rice and its applications. Specifically, this invention provides a Bacillus belye AN6, classified and named as follows: Bacillus velezensis This invention provides a novel rice endophytic Bacillus bereaves strain AN6. Through isolation, screening, identification, and experimental verification, it was found that this strain exhibits broad-spectrum resistance to various plant pathogens, including rice bacterial blight and rice blast. This strain has the ability to inhibit multiple plant pathogens and adsorb heavy metals, which is of great significance for the biological control of rice diseases and the development of green agriculture. (CCTCC accession number: M 20231886)

The present disclosure is generally related to recombinant microbial cells expressing heterologous proteins of interest. Certain aspects of the disclosure are therefore related to, inter alia, recombinant Bacillus cells having enhanced protein production capabilities, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and / or methods thereof. As exemplified herein, the recombinant Bacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.

This invention discloses a strain of *Bacillus videmannii* X3 and its application in inhibiting *Fusarium graminearum*, belonging to the field of microbial technology and its application. Strain X3 was isolated from the rhizosphere soil of the highly resistant wheat scab variety Sumai 3, with the preservation number CCTCC NO: M 20252108. Its mechanism of action is primarily through contact inhibition of the bacterial cells, supplemented by volatile substances. The innovative application effects of this invention are significant: in straw treatment at the source of the disease, it can reduce the abundance of soil pathogens by 71.04%; in direct prevention of wheat seedlings, the disease control efficacy can reach 67.58%. In summary, this invention not only provides a novel biocontrol strain resource but also constructs an innovative application system combining "source control" and "early prevention," providing key technical support for achieving "prevention and control, and integrated management" of wheat scab.

The application relates to an anti-Bacillus cereus nanobody and application thereof, the nanobody comprising a framework region FR and a complementarity determining region CDR, wherein the complementarity determining region CDR is CDR1 with an amino acid sequence as shown in SEQ ID NO. 6, CDR2 with an amino acid sequence as shown in SEQ ID NO. 7 and CDR3 with an amino acid sequence as shown in SEQ ID NO. 8. The nanobody can recognize and combine with Bacillus cereus, and has the advantages of easy expression and high expression efficiency, so that the nanobody can be applied to an enzyme-linked immunoassay method for detecting Bacillus cereus.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

The application discloses a bacteriocin expressed by bacillus subtilis WB800N and application thereof. The original sequence of the bacteriocin is a bacteriocin PA from bacillus polymyxa, the nucleotide sequence of the bacteriocin PA is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The bacteriocin PA coding gene is modified by a tag and optimized by a codon to obtain a bacteriocin PFH, the nucleotide sequence of the bacteriocin PFH is shown as SEQ ID NO:3, and the amino acid sequence is shown as SEQ ID NO:4. The bacteriocin PFH expressed by the bacillus subtilis WB800N has a good bacteriostatic effect on gram-positive bacteria such as streptococcus suis, enterococcus faecium, staphylococcus aureus and bacillus subtilis, and has a wide application prospect in the development of a new type of replacement product for infection treatment and a feed antibacterial peptide.

The invention relates to bacillus hanissii, which is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.37090. The strain has the effect of degrading ammonia nitrogen under high-temperature and aerobic conditions, and can be used for degrading ammonia nitrogen in a high-temperature environment. The degradation effect can be normally achieved under the condition that the water body temperature is 40-55 DEG C. Under the condition that the temperature is 55 DEG C, for the initial ammonia nitrogen concentration of 200 ppm, the ammonia nitrogen removal rate within 72 h is 92%.

This invention discloses a mushroom residue immobilized Bacillus mucilaginosus encapsulation agent and its application in pharmaceutical wastewater treatment. Specifically, the preparation method of the immobilized microbial strain of this invention includes three steps: mushroom residue carrier pretreatment and modification, Bacillus mucilaginosus activation and cultivation, and immobilization reaction. This invention uses widely available and low-cost mushroom residue as the raw material for nano-biochar. The selected solid waste mushroom residue is pretreated with an inorganic salt culture solution before drying. The carrier performance is further optimized through material modification, and then adsorbed and immobilized with a high-concentration Bacillus mucilaginosus bacterial solution. Specific excipients and processes are selected to improve the survival rate and performance stability of the immobilized agent.

This invention discloses a Bacillus subtilis lipase A mutant and its applications. Using wild-type Bacillus subtilis lipase A from existing literature as the research object, its amino acid sequence was designed and modified to obtain two mutant proteins, named BsLipA-M1 and BsLipA-M2, respectively. The Tm values ​​of BsLipA-M1 and BsLipA-M2 are 68.1℃ and 69.8℃, respectively, which are higher than those of WT (53.7℃), 20G6 mutant (49.4℃), and 5F12 mutant (60.1℃). Their activities are 1.30-2.75 times higher than the reported activities of 20G6 and 5F12 mutants. Therefore, the BsLipA-M1 and BsLipA-M2 mutants have significantly improved thermal stability and enzyme activity, which is more conducive to broadening their application range in oil and fat hydrolysis, enzymatic synthesis of biodiesel, or modification of food-grade oils.

This invention relates to a strain of *Bacillus wiedmannii* that inhibits plant pathogens and its applications, within the field of biological control. It aims to address the negative impacts of chemical control methods on the ecological environment and human health. The *Bacillus wiedmannii* strain is identified as *Bacillus wiedmannii* SJZ, deposited at the China Center for Type Culture Collection (CCTCC) on December 23, 2024, with accession number CCTCC NO: M 20242884. This *Bacillus wiedmannii* strain inhibits *Sclerotinia sclerotiorum* and *Heterophyllaria rubra*. It secretes proteases, cellulases, and chitinases. This *Bacillus wiedmannii* strain is also used for nitrogen fixation, secreting siderophores and IAA (inorganic acid oxidase).

This invention belongs to the fields of genetic engineering and bioengineering technology, specifically disclosing a method for synthesizing 2-keto-L-gulonic acid (2-KLG) using Bacillus as a substrate strain. This invention achieves single-strain, one-step fermentation for 2-KLG production using Bacillus as a substrate strain by introducing and expressing sorbitol dehydrogenase and sorbitol dehydrogenase with NAD(P)H or FADH as coenzymes in Bacillus. This invention provides a novel technical route for the microbial fermentation production of 2-KLG, with the potential to replace the traditional two-step fermentation process. It also provides reference and inspiration for the exploration and optimization of biosynthetic pathways for other important compounds.

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

The application belongs to the technical field of biology and relates to a Bysinum perfringens YF01 strain capable of producing an enzyme for catalyzing degradation of creatinine. The application also relates to a Bysinum perfringens YF01 preparation for degrading creatinine, which contains bacterial cells and / or crude enzymes of the Bysinum perfringens YF01 strain. Research results show that the Bysinum perfringens YF01 cell and the enzyme produced by the Bysinum perfringens YF01 cell can respectively degrade 500 mg / L of creatinine within 48 hours and 24 hours, and have important application prospects in reducing human blood creatinine levels and preventing and treating kidney diseases and corresponding complications.

The present application belongs to the technical field of mycotoxin biodegradation and bacillus velezensis, and particularly relates to a bacillus velezensis strain with degrading vomitoxin and multiple enzyme activities and application thereof. The strain is isolated from pig intestinal contents, and is identified by morphological identification, physiological and biochemical identification, and 16S rRNA and gyrB gene molecular identification, and is confirmed as bacillus velezensis, and is named as bacillus velezensis YK-19 (Bacillus velezensis YK-19). The strain has a degrading rate of vomitoxin (deoxynivalenol, DON) as high as 93.79%, and has multiple enzyme activities such as protease, amylase, beta-mannanase, xylanase, cellulase and pectinase, and does not have hemolytic activity, and has good biological safety. The strain also shows good acid resistance, bile salt resistance, salt resistance and high temperature resistance, and has a wide application prospect in actual production.

This invention relates to the field of plant disease control technology, and more particularly to a strain of Bacillus belye. Bacillus velezensis Application of YZ081609S19. This invention provides a *Bacillus belyceae* YZ081609S19, the Latin name of which is... Bacillus velezensis This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, on January 28, 2026, with accession number GDMCC No.: 67752. This strain exhibits significant inhibitory effects against various plant pathogenic fungi, particularly effectively inhibiting the pathogen causing sugarcane leaf spot disease. Simultaneously, this strain also promotes the growth of sugarcane plants.

The utility model relates to the technical field of granulation, specifically relates to a bacillus subtilis granulation equipment, including the granulating disc, the granulating disc outer surface fixed mounting has the convex edge to the upwards, the small hole is opened to the convex edge, the small hole diameter is less than the granulation particle size, and the convex edge outside installs the collection cover, and the collection cover bottom fixed mounting has the bottom support plate, and the bottom support plate and the collection cover with the convex edge and the granulating disc between all leave the gap. The utility model discloses through the hole on the outer convex edge of the granulating disc, and sets up the collection cover on the outside, so that the powder material and the granular material can be separated through the small hole, and then the powder material is collected by the collection cover, and the powder material is no longer mixed in the granular material, thereby avoiding the influence on the granular material granulation forming.

This invention provides a Bacillus sicca Z4, Bacillus siamensis The strain Z4 was deposited on March 3, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37830, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The optimal fermentation medium for strain Z4 was 1000 mL of water, with yeast extract at 15 g / L, maltose at 20 g / L, and sodium chloride at 4 g / L. The optimal fermentation conditions were: 1% inoculum in a 250 mL Erlenmeyer flask (75 mL), temperature 35℃, pH 7, rotation speed 289 r / min, and incubation time 18 h. Strain Z4 antagonized the growth and spore germination of *Spodoptera litura*, the causal agent of sorghum smut. The aseptic fermentation filtrate of strain Z4, diluted 10-20 times, could be used to accelerate sorghum seed germination and emergence. The fermentation broth of strain Z4, diluted 10-100 times, could be used for the control of sorghum smut. This invention provides new biocontrol resources for the prevention and control of sorghum smut and other diseases.

The application provides a recombinant bacillus subtilis producing phage lytic enzyme LysJPP58 and a construction method and application thereof. The recombinant bacillus subtilis integrates the lytic enzyme LysJPP58 into the genome of a host bacterium bacillus subtilis, wherein the lytic enzyme LysJPP58 is derived from a staphylococcus aureus phage, and an amino acid sequence of the lytic enzyme LysJPP58 is shown as SEQ ID NO. 1. The recombinant bacillus subtilis can effectively secrete the LysJPP58 protein with lytic activity, and can significantly lyse staphylococcus aureus. The recombinant bacillus subtilis has the characteristics of safety, sustainability and industrial fermentation production, and provides a new technical approach for the development of animal health, food safety and medical antibacterial preparations.