127 results about "Bacillus sp. BI" patented technology

The invention relates to the technical field of microbial technology and rare earth element recovery. The invention provides bacillus tequilensis Re2, and the preservation number of the bacillus tequilensis Re2 is CGMCC (China General Microbiological Culture Collection Center) No. 36764. The strain Re2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 25, 2025, the center is called CGMCC for short, the address of the center is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number of the strain Re2 is CGMCC No.36764, and the strain Re2 is classified and named as Bacillus tequilensis. The bacillus tequilensis provided by the invention can efficiently adsorb various rare earth elements, and the rare earth elements in spores can be eluted after 2, 6-dipicolinic acid (DPA) is added; therefore, the repeated utilization of the strain is realized.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

This invention discloses a compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis, its preparation method, and its application, belonging to the field of agricultural microbiology. The compound microbial agent comprises halophilic Bacillus and Bacillus belye, and an agriculturally acceptable carrier; wherein, single bacterial suspensions of halophilic Bacillus and Bacillus belye are mixed to obtain a compound bacterial suspension, and the compound bacterial suspension is further compounded with an agriculturally acceptable carrier to obtain the compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis. The halophilic Bacillus is classified and named... Bacillus halotolerans X14-2, classified as Bacillus belye Bacillus velezensis X147. This invention solves the problems of insufficient adaptability and poor growth-promoting effect caused by the single strain in the microbial agent in the prior art. It has the potential to prepare products that alleviate the continuous cropping obstacles of Scutellaria baicalensis or promote the growth of Scutellaria baicalensis.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

This present invention relates to compositions and methods of microbial enhanced oil recovery using Bacillus subtilis strains. The invention also relates to compositions and methods for performing oil degradation with Bacillus subtilis strains. The compositions and methods of the present invention are also used for enhanced commercial biosurfactant and enzyme production.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

This invention discloses an alkali-resistant xanthan gum side-chain lyase mutant and its preparation method, which is effective against alkali-resistant xanthan gum from Bacillus subtilis (Bacillus spp.). Paenibacillus nanensis Site-directed mutagenesis was performed on wild-type xanthan gum side-chain lyase to obtain alkali-resistant xanthan gum side-chain lyase mutants. The site-directed mutagenesis included at least one of Y260V, T387A, and I422S. The xanthan gum side-chain lyase mutants of this invention showed significantly increased enzyme activity compared to the original enzyme after treatment with an alkaline buffer system at 40°C, with the three mutants Y260V / T387A / I422S showing the greatest increase. These mutants are suitable for large-scale degradation of xanthan gum side chains under alkaline conditions, possessing significant social and economic benefits and broad application prospects.

The present application belongs to the field of microbial preparation, and discloses a kind of microbial stabilizer, including non-bacillus or its fermentation bacteria liquid, chemical stabilizer, wherein, the non-bacillus includes at least one of Serratia entomophila, Acinetobacter calcoaceticus.The present application also discloses the preparation method of the microbial stabilizer and its application in prolonging the shelf life of microbial agent.The present application studies the microbial stabilizer containing non-bacillus, finds that the microbial stabilizer formed by the suitable collocation of non-bacillus and chemical stabilizer can maintain the stability of viable bacterial concentration for a long time, can prolong the shelf life of microbial product, reduces the need for frequent production and storage, can ensure the supply of product in market more stable, thereby directly reduces the production cost, improves economic benefit, can be used in the field of microbial preparation technology.

The present disclosure is generally related to recombinant microbial cells expressing heterologous proteins of interest. Certain aspects of the disclosure are therefore related to, inter alia, recombinant Bacillus cells having enhanced protein production capabilities, novel protein signal sequences, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and / or methods thereof. Thus, as exemplified herein, the recombinant Bacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.

The present application relates to the technical field of strain propagation, in particular to a bacillus propagation device, which comprises a container and a sealing assembly, the container is coaxially provided with a first fixing ring and a second fixing ring, a first feeding hole is formed in the top end of the container, the sealing assembly comprises a sealing plug and a movable ring, three second feeding holes are formed in the sealing plug, the sealing plug works in cooperation with the first feeding hole, the sealing plug is slidably connected with the movable ring, the sliding direction of the sealing plug is parallel to the axis of the movable ring, a first fixing hole is formed in the first fixing ring, which can fix the position of the movable ring, a second fixing hole is formed in the second fixing ring, which can fix the position of the sealing plug when the sealing plug blocks the first feeding hole, and the second fixing hole can fix the position of the sealing plug when the sealing plug does not block the first feeding hole. The present application is convenient to seal the container by using the sealing assembly, and is also convenient to check and maintain the sealing assembly in case of failure.

The application discloses a bacillus zoogleoides BZ-32 and application of metabolites of the bacillus zoogleoides BZ-32 in vegetable stress resistance, and an effective component of the application is composed of bacillus zoogleoides fermentation liquor or metabolites, choline chloride and lentinan, wherein the weight percentage of the bacillus zoogleoides, choline chloride and lentinan is respectively 5-30% of bacillus zoogleoides metabolites, 5-30% of choline chloride and 0.2-5.0% of lentinan. The plant stress resistance composition can prevent and treat diseases such as root rot, anthracnose and powdery mildew of vegetables, and can prevent stress caused by excessively low temperature in winter and spring frost, and improve disease resistance and stress resistance of crops. The application can also be used for preventing and treating various corn diseases and strawberry diseases, and promoting growth and improving stress resistance.

This invention provides a method for mineralizing and recovering dysprosium ions from Bacillus pasteurellii, comprising the following steps: 1) Cell culture: Activated Bacillus pasteurellii is inoculated into CASO liquid medium and cultured for 22-26 hours, then centrifuged to collect the cell bodies; 2) Induced mineralization of Bacillus pasteurellii: The cell bodies collected in step 1) are mixed with a calcium source, urea, and dysprosium ions (Dysprosium). 3+ The dysprosium-containing precipitate was obtained by incubation with an inducing solution and filtration. This invention belongs to the field of bio-environmental protection technology. The method for mineralizing and recovering dysprosium ions using Bacillus pasteurellium provided by this invention can efficiently mineralize rare earth ions Dysprosium. 3+ No need to deal with Dy 3+ After pretreatment of the solution and biomineralization, the dysprosium-containing precipitate can be directly obtained by filtration without centrifugation, making the operation simple. 3+ The recovery rate is as high as 98.4%.

The present application relates to the field of microbial fermentation and bioengineering technology, and particularly relates to bacillus licheniformis and application thereof in acetoin and tetramethylpyrazine production. A bacillus licheniformis with high acetoin yield is screened from a large number of strains. The acetoin yield of the strain can reach 98 g / L after 36 h fermentation, which is much higher than that of other natural screening strains, and the liquor yield is increased by 11% and the content of tetramethylpyrazine in the liquor is increased by 57% when the strain is used for liquor brewing, which obviously improves the quality of the liquor.

The invention discloses a microbial fuel cell based on bacillus pasteurii as well as a construction method and application thereof, and relates to the technical field of biological fuel cells. The microbial fuel cell comprises a single-chamber reactor, an anode, a cathode and a wire, the cathode is positioned on the upper layer of the single chamber of the single-chamber reactor and is in contact with air; the anode is positioned at the single-chamber bottom layer of the single-chamber reactor; the lead is connected with the anode and the cathode; a cavity of the single-chamber reactor is filled with soil or an electrolyte solution; the surface of the anode is loaded with a bacillus pasteurii biological membrane; the bottom layer of a single chamber of the single-chamber reactor is loaded with a urea-containing culture medium. The microbial fuel cell provided by the invention can realize collaborative implementation of soil heavy metal remediation and electric energy recovery, and has the advantages of simple preparation process, stable electricity generation performance, high heavy metal removal efficiency, wide application range and the like.

The invention belongs to the technical field of gene engineering, and relates to a plasmid of bacillus, an artificial recombinant vector as well as a construction method and application thereof, the plasmid is called pBso for short, and the nucleotide sequence of the plasmid is shown as SEQ ID NO.1. In a plasmid sequence, a protein coded by an open reading frame is a replication element Rep protein of a gram-positive bacterium rolling circle replication type plasmid, and the nucleotide sequence of the protein is shown as SEQ ID NO.2. The artificial recombinant vector is constructed by carrying out gene recombination on plasmids of the bacillus. The construction method of the artificial recombinant vector comprises the following steps: cloning a replication element Rep protein by using a molecular cloning means to construct the artificial recombinant vector with neomycin resistance. The replication element Rep protein of the plasmid pBso of the bacillus can be used for constructing an artificial recombinant vector, stably exists in the bacillus subtilis and the bacillus amyloliquefaciens and is subjected to replication and passage, and the steps of obtaining the engineering bacillus are simplified.

The invention discloses a bacillus velezensis strain and an application of the bacillus velezensis strain. The bacillus velezensis strain M4 is obtained by screening soybean paste. The strain is preserved in China Center for Type Culture Collection. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 20251427; according to the present invention, the strain has characteristics of broad-spectrum antibacterial ability, high protease and cellulase production ability, and high alpha-amylase and gamma-aminobutyric acid (GABA) production ability, the protease yield is 318.96 + / -0.17 U / mL, and the cellulase yield is 52.63 + / -0.77 U / mL. In the process of fermenting the bean dregs, the bacillus velezensis M4 can inhibit the growth of pathogenic bacteria, obviously change the accumulation mode of amino acids, small peptides and analogues thereof in the bean dregs, improve the protein utilization rate of the bean dregs and obviously improve the flavor of the bean dregs.

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The invention discloses a method for crystallizing BM3 protein in bacillus megaterium. The method comprises the following steps: constructing a gene for coding the BM3 protein into an expression vector, carrying out induced expression in escherichia coli, carrying out affinity chromatography purification, excising a fusion tag, carrying out gel filtration purification, incubating with cofactors FAD, FMN and NADPH to form a compound, and crystallizing under a specific pool liquid condition through a seated drop gas phase diffusion method. According to the method, the high-quality BM3 protein crystal suitable for X-ray diffraction analysis can be obtained, and a basis is provided for analyzing the three-dimensional structure of the BM3 protein crystal and researching an electron transfer mechanism.

The invention discloses a bacillus strain NJAU-N3 and an application of the bacillus strain NJAU-N3. The invention relates to bacillus subtilis NJAU-N3, which is classified and named as bacillus cereus, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is August 18, 2025, and the preservation number is CGMCC No.35652. The bacillus subtilis NJAU-N3 is named as bacillus cereus, and is preserved in the China General Microbiological Culture Collection Center (CGMCC). The invention discloses the capability of the compound in soil nitrogen fixation and soybean growth promotion. The invention further discloses a microbial inoculum prepared from the bacillus subtilis and application of the microbial inoculum in soil nitrogen fixation and soybean growth promotion. The strain provided by the invention has wide temperature and drought adaptability, can play the functions of soil nitrogen fixation and soybean growth promotion in low-temperature and drought soil, is suitable for planting of crops such as soybeans in northeast semi-arid windy desert areas, and has good popularization and application values.

This invention belongs to the field of microbial technology, specifically relating to a type of endophytic Bacillus belye AN6 in rice and its applications. Specifically, this invention provides a Bacillus belye AN6, classified and named as follows: Bacillus velezensis This invention provides a novel rice endophytic Bacillus bereaves strain AN6. Through isolation, screening, identification, and experimental verification, it was found that this strain exhibits broad-spectrum resistance to various plant pathogens, including rice bacterial blight and rice blast. This strain has the ability to inhibit multiple plant pathogens and adsorb heavy metals, which is of great significance for the biological control of rice diseases and the development of green agriculture. (CCTCC accession number: M 20231886)

The invention discloses bacillus velezensis and application thereof, and relates to the technical field of biological control. The bacillus is a strain GZSF-g1B, and the preservation number of the bacillus is CGMCC (China General Microbiological Culture Collection Center) No.36194. According to the GZSF-g1B thallus suspension disclosed by the invention, indoor control effect determination is carried out on millet anthracnose by using the thallus suspension, and the control effect is 84.83%.

The present invention relates to a Bacillus licheniformis host cell for increased production of secreted proteins. Specifically, the invention relates to a Bacillus licheniformis host with a genetic modification that leads to increased production of a heterologous, non-native, secreted enzyme. Specifically, the present invention relates to a Bacillus licheniformis host cell expressing a) a first polypeptide having peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) activity, said first polypeptide comprising an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence being a least 81% identical to SEQ ID NO: 1, and b) a second polypeptide having amylase activity, wherein said second polypeptide is heterologous to said Bacillus licheniformis host cell.

The invention relates to a method for producing a trypsin-like protease in Bacillus, the method comprising expressing in a Bacillus host cell a gene coding for a preproenzyme comprising or consisting of (i) a trypsin-like protease having a sequence identity of at least 70% compared to the wild-type trypsin-like protease from the fungus Fusarium oxysporum, and (ii) a prosequence located upstream of the trypsin-like protease and having at its C-terminus a basic amino acid residue, and (iii) a bacterial secretion signal sequence located upstream of the trypsin-like protease and of the optionally present prosequence, wherein the bacterial secretion signal sequence is capable of directing secretion in a Bacillus host cell to a culture medium, thereby providing a culture medium comprising the mature trypsin-like protease.

The invention discloses bacillus velezensis YHBLS-1 and application thereof in degradation of nitrite and inhibition of soil pathogenic mould, and belongs to the technical field of microorganisms. The preservation number of the bacillus velezensis YHBLS-1 is CCTCC (China Center For Type Culture Collection) NO: M20251258, the bacterial colony of the bacillus velezensis YHBLS-1 is milky white and opaque, the edge of the bacterial colony is wrinkled and irregular, and the surface of the bacterial colony is rough; the bacillus velezensis YHBLS-1 and the microbial inoculum prepared from the bacillus velezensis YHBLS-1 have the capability of degrading nitrite, can effectively improve the water quality index of aquaculture and also can inhibit various soil pathogenic mycetes, and the strain is expected to be expanded to prevention and treatment of branchiomycosis and saprolegniasis in aquaculture, and can be used for preventing and treating various soil pathogenic mycetes. According to the bacillus velezensis YHBLS-1, the adverse effect on a water body is avoided, meanwhile, the disease infection rate of aquatic animals is reduced, the application range of the strain is widened, and the bacillus velezensis YHBLS-1 has economic value and a very good application market prospect.