472 results about "Bacillus sp. BI" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus velezensis for producing alpha-amylase from a tobacco source and application of the bacillus velezensis, and belongs to the technical field of microorganisms. The bacillus velezensis for producing the alpha-amylase from the tobacco source is A8-1, and the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M 20232632. The activity of the alpha-amylase produced by the bacillus velezensis A8-1 can reach up to 147.85 + / -0.05 U / mL at the high temperature of 70 DEG C through determination, and the content of tobacco starch in tobacco products can be effectively reduced when the alpha-amylase is applied to the tobacco products, so that the quality of the tobacco products is improved. On one hand, the quality, applicability and utilization rate of low-grade and low-grade tobacco leaves can be improved; on the other hand, the quality grade of tobacco leaves can be improved, and defects of high-quality tobacco leaves are overcome. Meanwhile, valuable microbial resources are provided for the tobacco industry, and a new technology is provided for promoting high-quality development of tobacco industry enterprises.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

The invention belongs to the technical field of potato scab prevention and treatment, and particularly relates to a complex microbial inoculant for preventing and treating potato scab and application of the complex microbial inoculant. The complex microbial inoculant provided by the invention comprises bacillus velezensis BEV2 and bacillus atrophaeus FM2-4, wherein the effective viable bacterium concentration of the bacillus velezensis BEV2 and the effective viable bacterium concentration of the bacillus atrophaeus FM2-4 in the complex microbial inoculant are respectively 1-3 * 10 < 8 > CFU / m. The complex microbial inoculant provided by the invention can effectively prevent and treat the potato scab, reduce the morbidity of the potato scab and improve the yield and quality of potatoes, and has the advantages of being not easily influenced by the environment and being harmless to the environment.

The invention discloses a bacillus subtilis strain and application thereof, the bacillus subtilis strain is bacillus subtilis X-NY1 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20242038. Bacillus subtilis X-NY1, bacillus velezensis and bacillus amyloliquefaciens are compounded into a microbial agent, a film-forming agent, a dispersing agent, a coloring agent, a stabilizing agent, a preservative, a conditioning agent and a nutritional agent are further added to prepare the compound microbial seed coating agent, the effective viable count of the microbial agent is 108-1011, and the quality guarantee period is longer than 6 months. The compound microbial seed coating agent can improve the plant height, root length, fresh weight and the like in the growth process of pennisetum alopecuroides, can effectively improve the stress resistance of the pennisetum alopecuroides under the salinization condition, and is small in addition amount, low in cost, safe, reliable, obvious in growth promoting effect and suitable for application and popularization.

The present application relates to the field of biotechnology, and discloses a strain of Bacillus velezensis, which is classified and named Bacillus velezensis ZM2024, and the deposit number is CCTCC NO: M2024695; the deposit date is April 15, 2024, and it is deposited in the China Center for Type Culture Collection. The Bacillus velezensis in the present application has good stress resistance and safety, high production of cellulase, and can significantly improve the nutritional content and feeding value of high-fiber plant feed raw materials such as palm meal; at the same time, the Bacillus velezensis can inhibit diarrhea-causing pathogens of livestock and poultry, and is suitable for livestock and poultry feed processing and feeding. In addition, the present application also discloses the use of the above-mentioned Bacillus velezensis and a feed.

The invention provides a culture medium formula capable of effectively improving vibrio inhibition capability of bacillus. The culture medium formula comprises 10-20 g of corn starch, 2-10 g of yeast powder, 2-10 g of malt extract, 5-20 g of glycerin, 0.5-1 g of L-aspartic acid, 1 g of dipotassium phosphate, 0.3 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 1 g of calcium carbonate and 1000 mL of distilled water. When the culture medium is used for culturing bacillus capable of naturally inhibiting vibrio, the obtained culture has a better vibrio inhibiting effect.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention provides bacillus velezensis TYF-QPY-E98 for producing saccharifying enzyme, a bacterial agent and application of the bacterial agent, and belongs to the technical field of microorganisms. The bacillus velezensis TYF-QPY-E98 provided by the invention is classified and named as Bacillus velezensis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.30424, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 24, 2024. The saccharifying enzyme activity of the bacillus velezensis TYF-QPY-E98 provided by the invention can reach 2181 U / g, the saccharifying power can reach 713.12 mg / g.h, the liquefying power can reach 1.62, and the prepared enhanced yeast for making hard liquor is stronger in flavor.

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention discloses a biocontrol bacterium composition and application thereof in prevention and treatment of corn diseases, and relates to the technical field of biology. The biocontrol bacterium composition comprises bacillus velezensis (Bacillus velezensis) HPP-0-TS7 and bacillus subtilis (Bacillus subtilis) HPP-0-GZL7, and the biocontrol bacterium composition comprises bacillus velezensis (Bacillus velezensis) HPP-0-TS7 and bacillus subtilis (Bacillus subtilis) HPP-0-GZL7. The biocontrol bacterium composition provided by the invention shows excellent technical effects in the aspects of preventing and treating corn diseases and promoting plant growth, and the two strains have a synergistic effect, so that not only is the prevention and control capability on specific diseases enhanced, but also the antibacterial spectrum is expanded, and a reliable guarantee is provided for comprehensive prevention and treatment; a good interaction relationship exists between the two strains, and the two strains jointly play a role through different action mechanisms, so that the stress resistance and yield potential of corn are comprehensively improved.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention provides bacillus velezensis H5 capable of producing broad-spectrum antibacterial substances and application of the bacillus velezensis H5. The preservation number of the bacillus velezensis H5 in the China General Microbiological Culture Collection Center is CGMCC No.32671. The bacillus velezensis H5 is separated from sea mud in a specific sea area, can generate antibacterial substances with broad-spectrum antibacterial activity, and mainly comprises bacitracin, surfactin and mycostatin. The antibacterial substance of the bacillus velezensis H5 is applied to preservation of perishable fruits such as grapes and the like and mildew prevention of cereals such as corn and the like, and can effectively inhibit spoilage of the perishable fruits and synthesis of mycotoxins such as aflatoxin and the like, and the action effect of the antibacterial substance is obviously superior to that of a food preservative natamycin. The invention provides a novel, green, safe and broad-spectrum antibacterial substance for preservation and fresh-keeping of fruits and mildew prevention of grains, and has important application value.

A microorganism according to the present disclosure is a microorganism (accession number: NITE BP-03832) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04073) belonging to Bacillus velezensis, a microorganism (accession number: NITE BP-04126) belonging to Bacillus velezensis, or a variant of these having a plant disease prevention effect.

The invention discloses a method for inhibiting production of vomitoxin from bacillus cereus, which is characterized in that isoleucine is added into a substance needing to inhibit production of vomitoxin from bacillus cereus, preferably 5-20mM of isoleucine is added. According to the present invention, the content of the toxin is detected through the liquid chromatography-mass spectrometry, and the cytotoxicity experiment verification results show that the isoleucine can significantly inhibit the synthesis of the vomitoxin of the bacillus cereus in the culture medium and the matrix, and can effectively reduce the toxic effect of the toxin on HeLa cells; the invention provides a safe and efficient biological control means which is suitable for various food systems such as starch food, dairy products, meat products and the like. Isoleucine can be used as a novel non-toxic and harmless food additive and is low in cost; the use method is simple, the output of vomitoxin can be reduced by directly adding a trace amount of isoleucine, and the food flavor is not affected.

The invention provides a salt-tolerant bacillus and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29259. A fermentation product obtained by using the strain has remarkable effects of moisturizing, whitening, relieving, resisting oxidation, resisting wrinkles, controlling oil, removing acnes and tightening, and can be applied to cosmetics.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis NEAU-L05, application of the bacillus velezensis NEAU-L05, antibacterial cyclic lipopeptide Iturin A and application of the antibacterial cyclic lipopeptide Iturin A. The bacillus velezensis NEAU-L05 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 19, 2024, and the preservation number is CGMCC No.33130. The bacillus velezensis NEAU-L05 disclosed by the invention can be used for preparing the bacillus velezensis NEAU-L05. The bacteriostasis rate of the bacterial strain to botrytis cinerea of phytopathogen is 67.2%. The bacteriostatic cyclic lipopeptide ItuinA separated from the strain can improve the storage performance of berries, and a basis is provided for development of multiple biocontrol microbial agents with excellent performance.

The invention discloses bacillus subtilis and application thereof. The bacillus subtilis LDH-Y3 is obtained by being separated from Guangzhou commercially available Rakang goat milk pasteurized milk, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 64442. The bacterial strain is high in growth speed and high in activity, the maximum viable count reaches 9.36 log CFU / mL, and the bacterial strain has good gastrointestinal tract digestion resistance. The bacillus subtilis is high in cellulase production capacity, CMCase (Carboxymethyl Cellulase) reaches 39.66 U / mL, beta-Gase (beta-Gase) reaches 2.36 U / mL, and Fpase (Fpasease) reaches 11.37 U / mL. The strain has the capability of inhibiting helminthosporium convex hilum and helminthosporium longibrachiatum, and after solid state fermentation of corn straw, the content of neutral detergent fibers in the corn straw can be reduced, and the digestibility of the straw is improved.

The invention discloses bacillus simplex A16 which is registered and preserved in the China Center for Type Culture Collection on July 18, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241597. The application of the bacillus simplex in uranium enrichment comprises the following steps: inoculating a ring of bacillus simplex into a peptone fermentation culture medium, and culturing in a shaking table for 20-28 hours to obtain a bacterial solution with an OD600 value of 1.6-2.0; centrifuging the obtained bacterial liquid, discarding the supernatant, and washing the precipitate with sterile water for 2-4 times to obtain thalli; and under the aerobic condition, adding the obtained thalli into a uranium solution, and culturing in a shaking table for 2-24 hours. The invention provides bacillus simplex A16, which can quickly remove uranyl ions in a water body, effectively reduce the concentration of uranium and achieve the purpose of repairing the uranium-polluted water body.

The invention belongs to the technical field of biology, and particularly relates to recombinant bacillus subtilis, a preparation method of fermentation liquor, immobilized cells, a preparation method of the immobilized cells, a method for synthesizing D-tagatose and application of the D-tagatose. The invention provides a strain of recombinant bacillus subtilis BLCY-012, and the preservation number of the recombinant bacillus subtilis BLCY-012 is CGMCC (China General Microbiological Culture Collection Center) No.31950. The recombinant bacillus subtilis BLCY-012 disclosed by the invention can be fermented to generate the D-tagatose-4-epimerase, the D-tagatose-4-epimerase is an intracellular enzyme, the D-tagatose-4-epimerase can be used for preparing the D-tagatose by taking the D-fructose as a raw material, the immobilized cell is prepared by utilizing the recombinant bacillus subtilis BLCY-012, and the recombinant bacillus subtilis BLCY-012 can be used for preparing the D-tagatose. The immobilized cell is used as an enzyme carrier to convert D-fructose into D-tagatose, the conversion rate can reach 28%-32%, and the conversion rate is high.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus velezensis DZ-4-002 and application thereof. According to the bacillus velezensis DZ-4-002 provided by the invention, the Latin name is bacillus velezensis, and the preservation number of the bacillus velezensis DZ-4-002 is CGMCC (China General Microbiological Culture Collection Center) No.25955. The strain not only can produce nitrite reductase at high yield, but also can improve the growth performance of animals and the milk production performance of cows. Within the experimental range of the invention, the bacillus velezensis provided by the invention not only can improve the egg production increase rate, the average egg weight and the feed utilization rate of laying hens, but also can improve the average daily gain, the average daily feed intake and the feed utilization rate of weaned piglets, and the average milk yield, the milk-feed ratio and the lactose content in milk of dairy cows. The method has a good application prospect in the fields of nitrite degradation agents, feeds, additives of the feeds and the like.

The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.

The invention belongs to the field of microbial preparations, and discloses a microbial stabilizer which comprises non-bacillus or a zymophyte solution thereof and a chemical stabilizer, and the non-bacillus comprises at least one of serratia nematophila and acinetobacter calcoaceticus. The invention also discloses a preparation method of the microbial stabilizer and an application of the microbial stabilizer in prolonging the shelf life of a microbial agent. The research on the microbial stabilizer containing non-bacillus discovers that the microbial stabilizer formed by proper matching of the non-bacillus and the chemical stabilizer can keep the stability of the concentration of viable bacteria for a long time, prolong the shelf life of microbial products and reduce the requirements of frequent production and storage; therefore, the production cost is directly reduced, the economic benefit is improved, and the method can be used in the technical field of microbial preparations.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The present invention provides an improved Bacillus cell having reduced expression of a metalloprotease gene. Inactivation of the metalloprotease gene reduces proteolytic degradation of a protein of interest produced by said Bacillus cell and thus, improves the yield of the protein of interest in such production method. The present invention is also directed to a method of inactivating the metalloprotease and the application of this method of inactivation when producing a protein of interest.

The invention discloses bacillus Witzmannii for inhibiting phytopathogen and application of the bacillus Witzmannii, relates to the field of biological prevention and control, and aims to solve the problem that chemical prevention and control methods of phytopathogen cause negative effects on ecological environment and human health. The bacillus wiedmannii is bacillus wiedmannii SJZ and is preserved in the China Center for Type Culture Collection, the preservation date is December 23, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20242884. The bacillus vetzmannii disclosed by the invention can be used for inhibiting sclerotinia sclerotiorum and solanum nigrum. And protease, cellulase and chitinase can be secreted. The bacillus vetzmannii is used for nitrogen fixation and can secrete siderophores and IAA (indoleacetic acid).

The present application relates to a novel Bacillus promoter variant and a protein production method using same. When the novel Bacillus promoter variant of the present application is used, the expression of a target protein can be significantly increased in a host cell, and thus the novel Bacillus promoter variant can be effectively used in the production of biotechnologically, medically, and industrially useful proteins through the mass production of proteins and target proteins such as polypeptide drugs.

The invention discloses bacillus subtilis and application thereof, and relates to the technical field of microorganisms, the bacillus subtilis S1-17L provided by the invention is preserved in the China General Microbiological Culture Collection Center, the strain preservation number is GGMCC NO: 31323, the preservation date is July 16, 2024, and the 16S rDNA sequence of the bacillus subtilis S1-17L is as shown in SEQ ID NO.1; the strain can secrete a plurality of hydrolases including protease, phosphatase and cellulase, has relatively high capacity of dissolving insoluble phosphorus in soil and a plurality of biological functions, is a growth-promoting and environment-friendly strain, and lays a foundation for research and development of efficient and green microbial fertilizers.