221 results about "Bacillus sp. BI" patented technology

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention discloses a method for inhibiting production of vomitoxin from bacillus cereus, which is characterized in that isoleucine is added into a substance needing to inhibit production of vomitoxin from bacillus cereus, preferably 5-20mM of isoleucine is added. According to the present invention, the content of the toxin is detected through the liquid chromatography-mass spectrometry, and the cytotoxicity experiment verification results show that the isoleucine can significantly inhibit the synthesis of the vomitoxin of the bacillus cereus in the culture medium and the matrix, and can effectively reduce the toxic effect of the toxin on HeLa cells; the invention provides a safe and efficient biological control means which is suitable for various food systems such as starch food, dairy products, meat products and the like. Isoleucine can be used as a novel non-toxic and harmless food additive and is low in cost; the use method is simple, the output of vomitoxin can be reduced by directly adding a trace amount of isoleucine, and the food flavor is not affected.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention relates to the technical field of microbial technology and rare earth element recovery. The invention provides bacillus tequilensis Re2, and the preservation number of the bacillus tequilensis Re2 is CGMCC (China General Microbiological Culture Collection Center) No. 36764. The strain Re2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 25, 2025, the center is called CGMCC for short, the address of the center is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number of the strain Re2 is CGMCC No.36764, and the strain Re2 is classified and named as Bacillus tequilensis. The bacillus tequilensis provided by the invention can efficiently adsorb various rare earth elements, and the rare earth elements in spores can be eluted after 2, 6-dipicolinic acid (DPA) is added; therefore, the repeated utilization of the strain is realized.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention discloses bacillus subtilis Bs1 as well as an antagonistic substance and application thereof. According to the invention, Bs1 with antagonism on colletotrichum gloeosporioides is separated and screened for the first time, an antagonistic substance is clear as Bs1 serine-producing protease Vpr, a Bs1 bacteriostasis mechanism and biological control potential excavation are analyzed in a multi-omics manner, a peripheral colonization rule of Bs1-Rif is clear, and an indoor control strategy of the strain Bs1 on cunninghamia lanceolata anthracnose is optimized. When the strain Bs1 is applied on the day when pathogenic bacteria are inoculated, a certain prevention and treatment effect is achieved, and it is indicated that the strain Bs1 can rapidly occupy the ecological niche; the average antibacterial rate of the strain Bs1 can reach 66.69%. The biocontrol strain and theoretical basis are provided for prevention and control of Chinese fir diseases, the application of biological prevention and control in forestry is promoted, the interaction mechanism of biocontrol bacteria and pathogenic bacteria can be deeply understood, and the sustainable development requirement of modern forestry is met.

The invention relates to the technical field of microbial preparations, and particularly discloses bacillus velezensis HY16 fermentation liquor freeze-dried powder and a preparation method and application thereof.The preparation method includes the steps that hydroxyl of beta-cyclodextrin reacts with anhydride of maleic anhydride, double bonds are introduced to beta-cyclodextrin molecules, and then under the action of persulfate serving as an initiator, the beta-cyclodextrin and maleic anhydride react with each other to prepare beta-cyclodextrin HY16 fermentation liquor freeze-dried powder; and carrying out free radical copolymerization reaction on the beta-cyclodextrin and 2-acrylamide-2-methylpropanesulfonic acid to obtain the modified beta-cyclodextrin. Sulfonic acid groups in 2-acrylamide-2-methylpropanesulfonic acid are strong hydrophilic groups, the water solubility of beta-cyclodextrin can be remarkably improved, growth of ice crystals during freezing is delayed, mechanical damage of the ice crystals to strains is reduced, and meanwhile negative charges of the sulfonic acid groups can be effectively absorbed through electrostatic repulsion. The freeze-drying process is simple, aggregation and inactivation of fermentation active substances or strains in the freeze-drying process are prevented, the survival rate of viable bacteria in the freeze-drying powder is increased, the stability of active ingredients in fermentation liquor is kept, and then the algae-lysing effect is improved.

The invention relates to the field of microorganisms, and discloses a strain of bacillus aryabhattai, which is characterized in that the preservation number of the bacillus aryabhattai is CCTCC (China Center for Type Culture Collection) NO: M 2024282. The bacillus aryabhattai provided by the invention can inhibit the activity of the pathogenic bacterium fusarium oxysporum f.sp.cubense of banana wilt, so that the banana wilt can be prevented and treated, and in addition, the bacillus aryabhattai also has a remarkable growth promoting effect on banana plants.

The invention discloses bacillus cereus with wide pH adaptability and application of the bacillus cereus in soil or underground water heavy metal remediation, the strain is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20241939), and the bacillus cereus is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (CCTCC). The strain is high in environmental adaptability and high in remediation efficiency, can show good reduction and endurance capacity to various heavy metal pollutants under the wide environmental conditions that the pH ranges from 4 to 10 and the temperature ranges from 16 DEG C to 37 DEG C, and is particularly suitable for in-situ remediation of high-concentration contaminated soil; experimental results represented by hexavalent chromium show that the removal rate of the strain in 1000 mg / kg hexavalent chromium contaminated soil within 7 days can reach 70-100%, the dependence of an existing microbial remediation technology on a low-concentration and alkaline environment or a long remediation period is broken through, and an efficient solution is provided for green bioremediation of multiple types of heavy metal contaminated sites.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

This invention discloses a compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis, its preparation method, and its application, belonging to the field of agricultural microbiology. The compound microbial agent comprises halophilic Bacillus and Bacillus belye, and an agriculturally acceptable carrier; wherein, single bacterial suspensions of halophilic Bacillus and Bacillus belye are mixed to obtain a compound bacterial suspension, and the compound bacterial suspension is further compounded with an agriculturally acceptable carrier to obtain the compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis. The halophilic Bacillus is classified and named... Bacillus halotolerans X14-2, classified as Bacillus belye Bacillus velezensis X147. This invention solves the problems of insufficient adaptability and poor growth-promoting effect caused by the single strain in the microbial agent in the prior art. It has the potential to prepare products that alleviate the continuous cropping obstacles of Scutellaria baicalensis or promote the growth of Scutellaria baicalensis.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

To provide a new application for an oral composition comprising vesicles separated from a food fermented from soybeans using a bacterium belonging to the genus Bacillus.SOLUTION: An oral composition containing vesicles separated from a food fermented from soybeans using a bacterium belonging to the genus Bacillus is used for at least one application selected from the group consisting of suppression of interleukin-1β production, improvement of diseases associated with interleukin-1β, and prevention of diseases associated with interleukin-1β.SELECTED DRAWING: None

An object is to obtain a β-amylase having heat resistance even to high temperatures exceeding 60° C. By screening β-amylase producing bacteria from a soil sample, a novel Bacillus halosaccharovorans strain having a novel β-amylase with heat resistance and the β-amylase from the novel B. halosaccharovorans strain were obtained.

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The invention relates to the technical field of microorganisms, in particular to a saline-alkaline-tolerant bacillus velezensis JT6, a cellulose degrading enzyme secreted by the same and application of the saline-alkaline-tolerant bacillus velezensis JT6. The bacillus velezensis JT6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 5, 2025, and the preservation number is CGMCC No.36918. The bacillus velezensis JT6 disclosed by the invention can be used for preparing the bacillus velezensis JT6. The strain has cellulose degradation activity, the degradation rate of corn straw is 48.8%, and the degradation rate of rice straw is 24.92%. The strain provided by the invention is used for rapid decomposition of straw returning, is safe to human and livestock, has no pollution to the environment, and has good development and application prospects.

The invention discloses a bacillus velezensis fermentation process, which comprises the steps of bacillus velezensis slant strain preparation, seed solution preparation, fermentation broth preparation and the like, the titer of the bacillus velezensis fermentation broth prepared by the method is 100-160CFU / mL, the spore content of the fermentation broth is obviously improved, and the bacillus velezensis fermentation broth has the advantages of high yield, high yield and the like. Therefore, the biocontrol effect of the obtained fermentation liquor is improved; and on the other hand, the preparation method maximizes the utilization rate of the raw materials, shortens the fermentation period, is suitable for batch production, and improves the economic benefit.

The application discloses bacillus subtilis G190 and application thereof in Eriocheir sinensis breeding. After soybean meal is fermented by the bacillus subtilis G190, aquatic product feed is prepared, and the Eriocheir sinensis is bred by using the feed, so that the survival rate and flavor substances of the Eriocheir sinensis can be remarkably improved. The effective implementation of the research can provide scientific theoretical basis for the effect, regulation mechanism and popularization and application of the probiotic bacillus feed, and effectively serve further development of sustainable ecological breeding technology of the Eriocheir sinensis.

The invention discloses bacillus subtilis Q16 and application thereof, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252232. According to the invention, the bacillus subtilis Q16 is separated from the body of the large yellow croaker and is added into the feed for the large yellow croaker, so that the growth performance, digestion, oxidation resistance and immunity of the large yellow croaker can be remarkably improved, and the intestinal flora composition structure of the large yellow croaker and the gene expression related to immunity and metabolism are changed.

The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

This present invention relates to compositions and methods of microbial enhanced oil recovery using Bacillus subtilis strains. The invention also relates to compositions and methods for performing oil degradation with Bacillus subtilis strains. The compositions and methods of the present invention are also used for enhanced commercial biosurfactant and enzyme production.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

Provided are a strain of Bacillus velezensis EA19 and the use thereof. The strain is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO:M2023602. The strain has a relatively good treatment effect and prevention effect on stripe rust of Triticuma aestivum, and shows a comparable control efficacy to triadimefon. Moreover, the strain has an effect of reducing dosage while enhancing efficacy when used in combination with mefentrifluconazole. Therefore, the strain has important application values in the control of agricultural pests and diseases, provides a new way for green control of stripe rust of Triticuma aestivum, and has broad application prospects.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis utilizing lactic acid and application of the bacillus velezensis in white spirit production. The lactic acid utilization strain obtained through screening has good tolerance, can be applied to Baijiu brewing, can significantly reduce the lactic acid content in the seventh-round fermented grains of Maotai-flavor by about 44.9%, improves the propionic acid and ethyl propionate in Baijiu by about 10.3%, improves the ethyl propionate by about 23.7%, and is stable in effect.

The invention belongs to the technical field of environmental microorganisms and discloses a bacillus strain SY5, a microbial agent and application of the bacillus strain SY5 and the microbial agent in wastewater treatment, the strain belongs to bacillus sp. And is preserved in Guangdong Microbial Culture Collection Center on January 20, 2025, the preservation address is No. 59 building, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 65835. The invention also discloses a microbial agent taking the strain as an active component and application of the microbial agent in wastewater treatment, and the strain specifically enhances part of biological characteristics through domestication and screening; the prepared microbial agent can be directly added into a wastewater treatment system, pollutants such as organic matters, ammonia nitrogen, total nitrogen, tetracycline antibiotics and the like in wastewater can be synchronously treated, the efficiency is high, and the requirements of low cost and large-scale popularization and application can be met.

The invention belongs to the technical field of microbial fermentation, and particularly relates to bacillus velezensis and application thereof in preparation of ethyl laurate, farnesyl acetate and octanoic acid. The invention provides a bacillus velezensis strain which is preserved in the China General Microbiological Culture Collection Center on October 13, 2025, and the preservation number is CGMCC No. 36155. The bacillus velezensis strain has the advantages that the bacillus velezensis strain is preserved in the China General Microbiological Culture Collection Center on October 13, 2025; the address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing; ethyl laurate, farnesyl acetate and octanoic acid can be obtained by fermenting sugarcane juice with the fermentation broth. After subsequent extraction, the method can be used for industrial production.