40 results about "Irpex lacteus" patented technology

The fungus Irpex lacteus is efficiently cultured on a solid substrate with a high content of crystalline cellulose. The fungal culture can be used to produce cellulases that are useful in conversion of cellulose to sugars. The fungal culture can also be used to produce chitin, which is itself valuable and can also be converted to chitosan. Use of the fungal culture for co-production of cellulases and chitin is also described.

The invention belongs to the field of environmental microorganism, and relates to irpex lacteus and application thereof. The irpex lacteus is a strain IFP00606 and preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2017, and the preservation number is CGMCC No.14352. A degrading bacterium produced by the technical scheme can effectively repair tetracycline antibiotic residue polluted water, reduce the harm on a human body by the antibiotic residue and protect the ecological environment.

The invention relates to irpex lacteus LL210, application thereof and a biocontrol microbial agent. The irpex lacteus provided by the invention is classified and named as Irpex lacteus, and the preservation number of the Irpex lacteus is CGMCC No.21057. A strain is separated from tomato leaves, and is non-toxic and free of pathogenicity. A plate confrontation test shows that the strain can effectively inhibit mycelial growth and spore germination of tomato botrytis cinerea and tomato late blight, and can be used for antagonizing plant pathogenic fungi tomato botrytis cinerea and/or phytophthora. The biocontrol microbial agent prepared from the irpex lacteus LL210 is used for preventing and treating tomato diseases, the prevention and treatment effects on tomato botrytis cinerea and late blight reach 81.35% and 87.68%, the biocontrol microbial agent has a good disease prevention effect and good compatibility with the environment, and a new microbial resource is provided for biological prevention and treatment of diseases of plants such as tomatoes.

The invention belongs to the field of biological feed, and relates to a preparation method of recombinant manganese peroxidase and application of the recombinant manganese peroxidase in degradation ofChinese herbal medicine lignin. The method comprises the following steps: firstly, carrying out preference optimization on a gene sequence of manganese peroxidase coming from Irpex lacteus, carryingout PCR amplification by taking the optimized gene as a template, connecting the optimized gene with pREP, then converting the connected gene into escherichia coli competent cells to obtain recombinant shuttle plasmids, and introducing the recombinant shuttle plasmids into defective schizosaccharomyces pombe competent cells to obtain recombinant manganese peroxidase engineering bacteria; and performing culture to obtain a crude enzyme liquid, mixing the crude enzyme liquid with Chinese herbal medicines, and then adding calcium chloride, manganese sulfate, laccase, glucose oxidase and a small molecular substance activator for a multi-enzyme synergistic enzymolysis reaction. According to the method provided by the invention, a large amount of manganese peroxidase can be rapidly obtained, industrial production of the manganese peroxidase is realized, a good and stable peroxidase product is prepared, and effective degradation of Chinese herbal medicine lignin is realized.

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention relates to a complex microbial inoculant for degrading corn straws at low temperature. The complex microbial inoculant is prepared from bacillus cereus TK-2 (Bacillus cereus), bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus megatherium (Bacillus megatherium), Pseudomonas fragi (Pseudomonas fragi), Trichoderma viride (Trichoderma viride), Aspergillus niger (Aspergillus niger), Phanerochaete and Irpex lacteus (Irpex lacteus), and the complex microbial inoculant can be used for degrading corn straws at low temperature. The complex microbial inoculant formed by nine strains including bacillus cereus has the degradation efficiency of 38.71% at the indoor constant temperature of 15 DEG C, can efficiently degrade the corn straw and accelerate the decomposition time in the natural environment of 1-10 DEG C, has a high degradation rate, has the degradation rate of 35.93% after 30 days, and has excellent stability.

The invention provides application of irpex lacteus in preparing anti-hypoxia healthy products. The irpex lacteus is prepared by fermentation, water extraction, alcohol sedimentation and lyophilizing methods. Animal experiments prove that the irpex lacteus has a remarkable anti-hypoxia effect. The irpex lacteus has the advantages of simple preparation method, low cost, small toxic and side effect and remarkable anti-hypoxia effect, and is suitable for preparation of anti-hypoxia healthy foods or medicinal preparations.

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention provides an additive for a liquid nutrient medium for Irpex lacteus fermentation, relating to formula optimization of a liquid nutrient medium for Irpex lacteus fermentation. Calcium lignosulphonate and natural borneol are added into the broad liquid nutrient medium to enhance the transport efficiency of Irpex lacteus cells, and promote the absorption and utilization of the Irpex lacteus for nutrient substances, thereby enhancing the yield of the cellular metabolism products adenosin and polysaccharide and the dry weight of hypha. The total adenosin content is up to 0.135-0.149 g/L, which is enhanced by 8.2% on average as compared with the liquid nutrient medium without the additive; the total polysaccharide content is up to 2...381-2.605 g/L, which is enhanced by 11.0% on average as compared with the liquid nutrient medium without the additive; and the dry weight of hypha is up to 15.176-15.320 g/L, which is enhanced by 9.1% on average as compared with the liquid nutrient medium without the additive. The additive can be used as a liquid nutrient medium additive for industrially fermenting Irpex lacteus.

The invention provides an application of irpex lacteus in preparing anti-fatigue healthy products. The irpex lacteus extract is prepared by fermentation, water extraction, alcohol sedimentation and lyophilizing methods. Animal experiments prove that the irpex lacteus has a remarkable anti-fatigue effect. The irpex lacteus has the advantages of simple preparation method, low cost, small toxic and side effect and remarkable anti-fatigue effect, and is suitable for preparation of anti-fatigue healthy products.

The invention discloses Irpex lacteus and application thereof in degradation of waste branches in orchards, and relates to the technical field of microorganisms. The Irpex lacteus SDAU-B disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.23855, the preservation date is November 17, 2021, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Irpex lacteus provided by the invention has the capability of degrading lignin and cellulose, can improve the activity of degrading enzyme in waste piles, can play a degrading role to promote the increase of the content of ammonium nitrogen in the piles, and can improve the degradation efficiency of waste branches in orchards.

The invention relates to novel strains, namely, irpex lacteus (Irpex lacteus PCV1) and schizophyllum commune (schizophyllum commune SCF1) for preparing active dietary fibers by fermenting potato dregs, and a method for converting the potato dregs to synthesize the active dietary fibers through degradation of irpex lacteus PCV1 and schizophyllum commune SCF1. The Irpex lacteus PCV1 is the strain belonging to the polyporales, polyporaceae and the irpex lacteus genus; the Irpex lacteus PCV1 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 6, 2016,and is assigned with the accession number of CGMCC NO.12529. The schizophyllum commune SCF1 is the strain belonging to the agaricales, schizophyllaceae and the schizophyllum; the schizophyllum commune SCF1 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 6, 2016, and is assigned with the accession number of CGMCC NO.12530. The method comprises thefollowing steps: inoculating Irpex lacteus PCV1 into a liquid fermentation culture medium containing the potato dregs, brain and inorganic salt for fermentation culture, after culture is finished, inactivating the fermentation liquor, after inactivating, inoculating the schizophyllum commune SCF1 for fermentation culture, carrying out homogenization treatment on the fermentation final product, and thus the active dietary fiber product is obtained.

The invention discloses a method for extracting polypeptides from Irpex lacteus. The method comprises steps as follows: the Irpex lacteus is subjected to impurity removal, freezing and crushing, degreasing is performed with a supercritical CO2 fluid extraction method, then protein and peptide substances are extracted with a NaOH solution, and the polypeptides are extracted from the Irpex lacteus after further enzymolysis and ultrafiltration. The method is simple in technology, safe and free of pollution, the obtained product is high in purity and extraction rate, side reactions and addition products are few, and the method is easy to operate and popularize and suitable for industrial production.

The invention discloses irpex lacteus fermented tea. The irpex lacteus fermented tea is obtained by co-fermentation culture of irpex lacteus and tea leaves. Accordingly, the inventor also establishes a corresponding preparation method, and Irpex lacteus hyphae obtained through tea water culture are selected to be domesticated, cultured, overgrown and dried in the sun to obtain the Irpex lacteus fermented tea. The method comprises the following steps: inoculating Irpex lacteus into a culture medium containing tea leaves for fermentation culture, co-fermenting the domesticated Irpex lacteus and the tea leaves to form Irpex lacteus fermented tea, and generating a new secondary metabolite of the Irpex lacteus fermented tea in the process. A further research shows that the irpex lacteus fermented tea can significantly reduce the content of blood fat indexes TG, TC and LDL-C of hyperlipidemia model mice, significantly improve the HDL-C level, and has a good blood fat reducing effect on hyperlipidemia. Therefore, the irpex lacteus fermented tea has the blood fat reducing effect superior to that of the irpex lacteus and the tea leaves, and a theoretical basis is provided for deep research and development of the irpex lacteus and the irpex lacteus fermented tea.