15 results about "Irpex lacteus" patented technology

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant modified through semi-rational design and application of the manganese peroxidase mutant to straw degradation. On the basis of Irpex lacteus MnP, a core site G189 is screened out through structural analysis and alanine scanning, a G189A single mutant (glycine-alanine) is constructed, the G189A single mutant is free of Mn < 2 + > dependence, the enzyme activity reaches 15.2 U / mL (about 130% higher than that of a wild type), and the catalytic efficiency is improved by 2.1 times. Meanwhile, the mutant gene is introduced into bacillus subtilis to realize efficient extracellular secretion, the straw lignocellulose depolymerization can be effectively promoted, the application value in the fields of agricultural straw recycling and the like is remarkable, and the wide market application prospect is achieved.

The preparation method comprises the following steps: mixing a termite palatable cellulose material, wheat bran, gypsum, cane sugar and water to prepare a culture material, packaging and sterilizing the culture material, inoculating the Irpex lacteus, culturing until hyphae cover all the culture material, and carrying out freeze-drying to obtain the Irpex lacteus living fungus termite bait. The hypha living body state is kept for trapping odontotermes formosanus to eat. Experiments of three indoor devices show that the food calling effect of the Irpex lacteus bait block on odontotermes formosanus is obviously superior to that of a sterile culture material contrast bait block. Field tests show that the eating efficiency of the Irpex lacteus living fungus bait is obviously superior to that of natural plant bait circulating in the current market. Meanwhile, breathable tubular and bag-shaped packaging is convenient to store and carry, and hyphae can survive for a long time. The Irpex lacteus living fungus bait is good in termite trapping effect, high in eating speed, not prone to being polluted by infectious microbes and suitable for monitoring, trapping and killing odontotermes formosanus on dams and gardens.

The present application belongs to the field of microbial fermentation and microbial food technology, and particularly relates to a fermentation culture additive of Lactobacillus gasseri JM1 and use thereof. The fermentation culture additive is selected from at least two of polysaccharides of Irpex lacteus, Hericium erinaceus and Inonotus obliquus, or inactivated products of fermentation products of Lactobacillus gasseri JM1. The fermentation culture additive can promote the growth and proliferation of the strain when Lactobacillus gasseri JM1 is fermented. The present application also provides a beverage, and the preparation raw materials include milk powder, polysaccharide products and inactivated Lactobacillus gasseri JM1 blended fermentation products. The polysaccharides in the polysaccharide products are selected from at least two of polysaccharides of Irpex lacteus, Hericium erinaceus and Inonotus obliquus. The inactivated Lactobacillus gasseri JM1 blended fermentation products are added in the fermentation stage of Lactobacillus gasseri JM1, and the polysaccharide products are further added after beverage ingredients are prepared, which is beneficial to improve the viable count and physical stability of the beverage.

The invention discloses Irpex lacteus YYY-16, and the preservation number of the Irpex lacteus YYY-16 is GDMCC No: 67140. The invention further discloses a terpenoid and a preparation method thereof. The terpenoid has a structure shown in a formula I or a formula II. The invention further discloses application of the terpenoid in preparing tobacco pathogenic bacterium resisting drugs.

The invention provides Irpex lacteus S351, which is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Irpex lacteus S351 is CGMCC No.42285. The Irpex lacteus S351 is named as Irpex lacteus S351. According to the Irpex lacteus S351, waste orange peel capsule dressing can be used as a main substrate for mycelium growth, the surface of the obtained mycelium is pure white, the structure is compact, the Irpex lacteus S351 can be used for preparing degradable and environment-friendly packaging materials, and the prepared packaging materials are excellent in buffering performance.

The application discloses a white-cyst Irpex lacteus and application of the white-cyst Irpex lacteus in degradation of corn stalks and fermentation production of erucic acid amide. The white-cyst Irpex lacteus is classified and named as Irpex lacteus J2, and has been preserved in the China Center for Type Culture Collection on November 24, 2023, with a preservation number of M20232318. The strain has a complete lignocellulose-degrading enzyme system, and can produce erucic acid amide by taking corn stalks as the only carbon source.

The invention belongs to the technical field of lignin degradation, and discloses a composite lignin degrading fungus, a fermentation culture method, application and straw feed, the composite lignin degrading fungus, Irpex lacteus and Trametes versicolor are included, and the weight ratio of Irpex lacteus to Trametes versicolor is (1-2): (1-2). According to the composite lignin degrading fungus provided by the invention, Irpex lacteus and Trametes versicolor are adopted to form a composite strain, and when the Irpex lacteus and the Trametes versicolor are compounded, the effect of synergistically improving the lignin degrading capacity can be achieved.

The invention discloses Irpex lacteus and an application of the Irpex lacteus in production of erucyl amide by degrading corn straws through fermentation. The Irpex lacteus is classified and named as Irpex lacteus, the name of the Irpex lacteus is Irpex lacteus J2, the Irpex lacteus is preserved in the China Center for Type Culture Collection on November 24, 2023, and the preservation number of the Irpex lacteus is M20232318. The strain has a complete lignocellulose degrading enzyme system and is used for producing erucyl amide by taking corn straws as a unique carbon source.

The application discloses a reference gene of Irpex lacteus, primers and application thereof. The reference gene comprises ubiquitin conjugating enzyme 1, autophagy protein Atg8, ubiquitin conjugating enzyme 2, calmodulin-dependent phosphatase and fungal protein kinase gene. The five reference genes disclosed in the application are suitable for expression level research of different expression level genes. The reference gene provided by the application has stable expression in different development periods and different culture conditions, and the reference primers have high specificity, stable amplification and amplification efficiency close to 100%. In addition, according to the expression level of a target gene, a suitable reference gene can be selected from the five reference genes for expression level research. The application provides new candidate reference genes which are more stable and more extensive than common reference genes such as gapdh for gene expression research of Irpex lacteus.

The present invention provides a Bacillus velezensis SFT4 with antibacterial function and its application, belonging to the technical field of microbial application. Bacillus velezensis SFT4 has good inhibitory effects on Irpex lacteus, Aspergillus pallidus, Clostridium perfringens, Staphylococcus aureus, Micrococcus luteus, etc., and has a certain inhibitory effect on Salmonella. A probiotic preparation containing Bacillus velezensis SFT4 and its metabolites is provided, and a method for using the probiotic preparation is provided. The probiotic preparation is composed of Bacillus velezensis SFT4, Lactobacillus reuteri, Lactobacillus casei, and Lactobacillus plantarum. The application of the probiotic preparation in the livestock and poultry breeding industry can effectively prevent and control Clostridium perfringens and Escherichia coli, and the probiotic preparation can be made into a feed additive or directly fed through a drinking water line, and it is not easy to block the drinking and feeding pipeline.

The invention relates to the technical field of biology, in particular to Irpex lacteus nd6 and application of the Irpex lacteus nd6. The invention discloses Irpex lacteus nd6, and the preservation number of the Irpex lacteus nd6 is CGMCC (China General Microbiological Culture Collection Center) No.41795. The obtained irpex lacteus nd6 is used for fermentation of the caragana korshinskii rubbing chippings, and the result shows that the conventional nutrition of most caragana korshinskii is remarkably improved, the content of the important index crude protein of the feed is remarkably higher than that of an original sample, and the contents of neutral detergent fibers, acid detergent fibers and acid detergent lignin are remarkably reduced compared with the original sample group; and the degradation rates respectively reach 8.10%, 10.46% and 18.67%, which shows that the irpex lacteus nd6 has better application potential when being used for degrading the caragana microphylla lignocellulose.

The invention discloses a method for extracting flavonoid components from Irpex lacteus sporocarp, which comprises the following steps of: dipping and extracting by using a dichloromethane-methanol mixed solvent, extracting by using ethyl acetate, performing normal-phase silica gel column chromatography separation, performing gradient elution and purification and the like. And three flavonoid compounds, namely sakuranetin, 7-O-methyl-hesperidin and rhamnocitrin, are obtained through separation for the first time. An antioxidant activity test shows that the obtained compound has a remarkable scavenging effect on DPPH, ABTS and hydroxyl radicals, and the scavenging capacity on the hydroxyl radicals is superior to that of positive control Vc (ascorbic acid). The invention provides a new direction for deep development and utilization of Irpex lacteus, and the obtained flavonoid compound can be used for preparing natural antioxidants applied to the fields of foods, medicines or cosmetics.

The invention relates to a strontium-containing growth regulator and application thereof in improving stress resistance of plants, and belongs to the technical field of plant growth regulators. The growth regulator is composed of a component A and a component B. The component A contains 0.3-0.5 part by weight of jasmonic acid, 0.2-0.25 part by weight of ligustilide, 1-1.5 parts by weight of a bunge pricklyash leaf extract, 1-1.5 parts by weight of a Chinese prickly ash leaf extract, and the balance of water. The component B is prepared from 10 to 12 parts by weight of microbial extract, 0.1 to 0.3 part by weight of choline chloride, 15 to 20 parts by weight of potassium fulvate, 1 to 1.5 parts by weight of betaine, 5 to 8 parts by weight of Irpex lacteus polysaccharide, 100 parts by weight of water and 10 to 15 parts by weight of surfactant. When the growth regulator is used for soaking plant seeds or treating plant seedlings, the germination rate of the seeds in a low-temperature environment can be obviously increased, and the growth performance of the seedlings in the low-temperature environment is improved.

The present application relates to the technical field of fermentation, in particular to irpex lacteus and its application in the conversion of rare ginsenosides. The present application discovers and cultivates a wild irpex lacteus which can convert rare ginsenosides in panax notoginseng, further optimizes the irpex lacteus-panax notoginseng bidirectional solid fermentation process through experiments, and confirms that the process can efficiently convert common ginsenosides in panax notoginseng into rare ginsenosides; through pharmacodynamic evaluation, it is further shown that fermented panax notoginseng can significantly improve the pathological state of lupus nephritis model mice through multiple pathways such as immune regulation, inflammation inhibition and anti-kidney fibrosis, and its overall curative effect is better than that of panax notoginseng. The present application not only provides a new technical path for the deep development and value improvement of panax notoginseng, but also provides a potential candidate drug for the treatment of lupus nephritis, and embodies the wisdom of traditional Chinese medicine processing "synergistic and detoxification".