Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Irpex lacteus capable of efficiently degrading lignin and application of irpex lacteus

A technology of white sac rake tooth bacteria and lignin, if applied in this field, can solve the problems of low utilization rate and increase of livestock and poultry breeding waste, so as to improve immunity, good application prospects, improve digestibility and health care The effect of the function

Inactive Publication Date: 2016-07-13
JILIN AGRICULTURAL UNIV
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The crude protein content of feather meal obtained by hydrolyzing poultry feathers under high temperature and high pressure is as high as 80%, and also contains trace elements and even some growth factors, but the protein is mainly keratin, which has a stable chemical structure and is difficult to be digested by animals. Digested by the digestive enzymes in the feed, if it is directly added to the feed, it will cause low utilization rate and increase the pollution level of odorous substances in livestock and poultry farming waste

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Irpex lacteus capable of efficiently degrading lignin and application of irpex lacteus
  • Irpex lacteus capable of efficiently degrading lignin and application of irpex lacteus
  • Irpex lacteus capable of efficiently degrading lignin and application of irpex lacteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The experiment of the degradative effect of ZK1 albicans rake-tooth fungus on straw skin.

[0050] Separate the dried corn stalks from the skins, take the straw skins with the same position and appearance observation results, cut them into strips with a length of 5 cm and a width of 0.5 cm, add them to the test tube, add inorganic nutrient salts to moisten the straw, and inoculate 1 Cultivate a fungus cake, cultivate until the straw is covered with mycelia, stop the cultivation, gently remove the mycelium on the straw skin, clean the straw in an ultrasonic cleaner for 10-15 minutes, and then rinse repeatedly with sterile water , please gently blot dry with filter paper, then dry at 40°C for 2 hours, and observe with a scanning electron microscope, the results are as follows image 3 shown. It can be seen from the figure that compared with the control, the straw husk has significantly more small holes, indicating that the fungus can decompose the lignin on the surface a...

Embodiment 2

[0052] An experiment for analyzing the enzyme production characteristics of ZK1 albicans albicans of the present invention.

[0053] Strain ZK1 was inoculated into PDA solid medium for cultivation, and a 7mm bacterial cake was prepared, and 3 pieces of bacterial cake were added to 100mL enzyme-producing medium for cultivation at 30°C, and samples were collected regularly to determine the activity of the enzyme. Utilize AzureB method, manganese sulfate method and ABTS method to measure the activity of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) respectively, the result is as follows Figure 4 shown. It can be seen from the figure that the strain ZK1 can produce laccase, lignin peroxidase and manganese peroxidase in the basal medium, and the activities of the three enzymes are 13.4U / mL, 10.6U / mL, 9.24U / mL, respectively. U / mL, indicating that strain ZK1 has the strongest ability to produce laccase, followed by manganese peroxide plum, and the ability to...

Embodiment 3

[0056] The analysis experiment of the release ability of Phytate Phosphorus by Raketodon albicans of the present invention.

[0057] Inoculate 3 pieces of ZK1 bacteria cake into calcium phytate liquid medium, culture at 30°C and 150r / min, take samples every 5 days, and measure the content of phosphate radicals, the results are as follows Figure 5 shown. With the prolongation of culture time, the amount of phosphate ion released by strain ZK1 gradually increased. By the 20th day, the concentration of soluble phosphate released by strain ZK1 from calcium phytate was as high as 680mg / L, which was 7.5 times that of the control group, indicating that the strain ZK1 In the process of utilizing straw, active phosphate ions may be released from phytate phosphorus, which can improve the utilization rate of phosphorus in straw, thereby reducing the content of phosphorus in waste, which is very important for reducing the eutrophication of water caused by livestock and poultry farming. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to irpex lacteus capable of efficiently degrading lignin and application of irpex lacteus. The irpex lacteus is preserved in the China General Microbiological Culture Collection Center, and the preservation No. is CGMCC No.11920. The ZK1 irpex lacteus can be adopted to ferment straw composite feed prepared from a mixed material of straw powder, feather powder and oyster shell powder, the content of polysaccharide of the straw composite feed is as high as 203.2 [mu]g / g, the content of real protein of the straw composite feed is as high as 72.68%, the pH value of the straw composite feed is kept within 7.5-8.5, the lignin degradation rate of the straw composite feed is as high as 62.45%, and the degradation rate of cellulose of the straw composite feed is as high as 45.48%, so that the digestion rate of the straw composite feed is increased, and the healthcare function of the straw composite feed is improved.

Description

[0001] Application field [0002] The present invention relates to the technical field of microbial application, in particular to a strain of Raketooth albicans and its application in straw feed Background technique [0003] Crop straw is an important renewable biomass resource, mainly composed of organic matter including lignin, cellulose and hemicellulose, water and a small amount of inorganic mineral elements, and is an important source of feed for livestock and poultry, especially ruminants . However, because lignin and hemicellulose are cross-linked with each other, and cellulose is embedded in it to form a strong ether bond or ester bond, it is not easy to be digested by animals or decomposed by microorganisms, and the nutrient conversion efficiency is low. Even so, the potential for the utilization of straw as feed is still huge. In recent years, my country has made some progress in the utilization of straw as feed. At present, it mainly includes physical, chemical, bi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A23K10/18A23K10/26A23K10/37C12R1/645
CPCC12N1/145C12R2001/645Y02P60/87
Inventor 张超李海燕陈光王雅雯井雨铂程锐
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com