29 results about "Lignin peroxidase" patented technology

The invention discloses a low-temperature-resistant and drought-resistant straw degrading bacterium and application thereof. The low-temperature-resistant and drought-resistant straw degrading bacterium is obtained through screening, is stenotrophomonas maltophilia GF-Y18, has good capacity of producing exon-beta-1, 4-glucanase, laccase and lignin peroxidase, can effectively promote straw decomposition, has certain low-temperature-resistant and drought-resistant capacity, can be used for degrading straw, and can be applied to the field of straw degradation. The strain and the fungicide thereof can be used for degrading lignin and / or cellulose and / or straw in a low-temperature drought environment.

The invention discloses a preparation method of an actinomycetes and waste mushroom matrix composite, belongs to the technical field of environmental microorganisms, solves the problems of low resource utilization rate, serious environmental pollution and the like in the existing waste mushroom matrix treatment, and overcomes the defects of loose soil structure and poor erosion resistance caused by direct returning of the waste mushroom matrix to the field. The method comprises the following steps: separating actinomycetes strains from soil, purifying and culturing the actinomycetes strains into a bacterial solution, crushing a waste mushroom substrate, adjusting the water content and the initial pH value, inoculating the bacterial solution into a pretreated substrate, and culturing in a dark place to form a composite material with biological activity and structural stability. Lignocellulose in a matrix is efficiently degraded by utilizing cellulase secreted by actinomycetes and lignin peroxidase, and a three-dimensional cross-linked structure is formed through a hypha network and humic acid-minerals, so that the mineralization efficiency of organic matters and soil agglomeration are improved. The composite material can be applied to the field of water and soil loss prevention and control, and efficient conversion of waste resources and collaborative improvement of soil ecological functions are achieved.

The invention discloses streptomyces pseudogriseogriseus for promoting straw degradation and increasing soil organic matters, and belongs to the technical field of microorganisms. The streptomyces pseudogriseolus NF3 disclosed by the invention has the activity of CMC-Na enzyme, xylanase, laccase, lignin peroxidase and manganese peroxidase, and can be used for degrading cellulose, hemicellulose and lignin at the same time, and the degradation rate of the streptomyces pseudogriseolus NF3 on corn straws reaches 67.75%; the fertilizer is applied to straw returning soil, soil nutrient release is accelerated, the soil organic matter content is increased, and the crop yield is increased.

This invention belongs to the field of resource utilization technology, specifically relating to a low-temperature composting self-heating start-up compound microbial agent, its preparation method, and its application. The low-temperature composting self-heating start-up compound microbial agent is composed of *Broodia buddingis* (…). Aureobasidium sp.X4-8) and Bacillus licheniformis ( Bacillus licheniformis The compound microbial agent (H-29) was prepared by combining two bacterial groups. The two bacterial groups exhibited dominance and functional complementarity at different temperature stages. When added at -30℃, the psychrophilic fungi initially "ignited" the heat, and as the compost temperature exceeded 40℃, the heat-resistant bacteria rapidly took over and released lignin peroxidase and manganese peroxidase, achieving continuous regulation of the composting system from initial heating and high-temperature maintenance to deep decomposition under low-temperature conditions. Aerobic composting of agricultural waste using the compound microbial agent resulted in a seed germination index (GI) ≥ 70% for the decomposed product, with all indicators meeting the NY / T 525-2021 standard.

The invention discloses a production technology of a jute environment-friendly coir mat with a three-dimensional structure, and relates to the technical field of jute coir mat production.The production technology comprises the following steps that S1, raw material pretreatment is conducted, specifically, raw jute is sequentially subjected to three-stage degumming including anionic surfactant solution soaking, pectinase treatment, lignin peroxidase treatment and caustic soda boiling-off, performing countercurrent cleaning and vacuum drying to obtain flexible fibers; s2, mixing fibrils: mixing the pretreated jute fibers with palm fibers and ramie fibers in proportion to form a mixed fiber raw material; s3, opening and carding: performing loosening treatment on the mixed fibers by a bidirectional opener, and preparing flocculent fibers by a carding machine; the flocculent fiber filaments are sprayed to a lapping machine through an exhaust fan, the lapping machine reciprocates at the constant speed of 1.5-2.5 m / s in the direction perpendicular to the spraying direction, the fiber flexibility can be improved, a three-dimensional supporting structure can be constructed, and a formaldehyde adding source can be eliminated.

The invention discloses an aflatoxin B1 degrading enzyme and application thereof. The degrading enzyme comprises lignin peroxidase, dye decoloring peroxidase and multifunctional peroxidase. According to the invention, an efficient and targeted aflatoxin B1 degrading enzyme screening model is established by adopting a molecular simulation technology, and the problems of high complexity, low screening efficiency and the like commonly existing in a traditional screening method are solved. The engineering strain provided by the invention takes escherichia coli Shuffle T7-B as a host, has a stable degrading enzyme expression characteristic, and ensures the efficient expression of the degrading enzyme. The degradation rate of the degrading enzyme provided by the invention on aflatoxin B1 reaches 84% or above.

The invention discloses a tea tree pruning efficient degradation microbial combined bacterium, a microbial agent and application of the microbial agent, the tea tree pruning efficient degradation microbial combined bacterium comprises bacillus JKB11 and paenibacillus polymyxa JKB16, and the JKB11 and JKB16 combination in the tea tree pruning efficient degradation microbial combined bacterium can effectively deal with degradation of tea tree pruning; although the living enzymes of cellulose degrading enzyme (CMCase) and lignin degrading enzyme (lignin peroxidase (LiP) and manganese peroxidase (MnP)) are similar to those of JKB16, the three-element content and infrared spectrum indexes of the degradation product prove that the tea tree pruning degradation effect of the combination of JKB11and JKB16 is better.

The method comprises the following steps: (1) raw material pretreatment: taking tobacco leaf powder after supercritical extraction, putting into a reaction kettle, adding water, uniformly stirring, adjusting the pH value to be 5-6, sealing, reacting for 1-2 hours at the temperature of 180-200 DEG C, cooling, transferring into an oil bath pan at the temperature of 50 DEG C, and uniformly stirring; (2) compound enzyme preparation activation and enzymolysis: taking a compound enzyme (manganese peroxidase, laccase and lignin peroxidase) accounting for 0.5% of the mass of the raw material, dissolving the compound enzyme in water, activating in a water bath at 50 DEG C, dropwise adding the pretreated raw material, performing oil bath enzymolysis at 50 DEG C for 3-4 hours, heating to 100 DEG C, stirring for 20 minutes, and cooling to obtain a feed liquid A; and (3) preparing the tobacco absolute, namely centrifuging the feed liquid A, taking supernatant liquid (feed liquid B), adding 95% ethanol, uniformly stirring, separating and removing impurities by using a cellulose acetate or polyacrylonitrile membrane, and collecting permeate liquid to obtain the tobacco absolute. The equipment is simple, the preparation method is green and efficient, and the prepared tobacco absolute can effectively improve the cigarette smoke quality.

The invention belongs to the field of biotechnology and biomass conversion, and particularly relates to a lignin peroxidase mutant based on semi-rational design, a preparation method of an enzyme preparation and application of the lignin peroxidase mutant and the enzyme preparation in straw degradation. According to the method, a three-dimensional model constructed by wild type lignin peroxidase is evaluated, a potential mutation hot spot is screened out in combination with a molecular docking technology, and then the high-activity lignin peroxidase mutant is successfully obtained through alanine scanning, saturated mutation and combined mutation three rounds. After an enzyme activity improvement mechanism is simulated and analyzed through molecular dynamics, an optimized high-activity mutant is optimized according to codon preference of pichia pastoris and then integrated into an expression vector pPIC9K of the pichia pastoris, so that secretory expression of the mutant is realized; the composite material shows significantly improved catalytic efficiency in straw degradation. While the outstanding degradation effect is achieved, the method also has important significance for promoting the green and efficient development of the feed industry.

The invention belongs to the technical field of refined cotton preparation, and particularly relates to a method for preparing refined cotton through chlorine-free bleaching. The preparation method comprises the following steps: putting cotton fibers subjected to soda boiling into a mixed solution containing hydrogen peroxide, amino trimethylene phosphonic acid, nano titanium dioxide and a nano composite chlorine-free bleaching agent, and treating by adopting plasma; then hydrogen peroxide, lignin peroxidase and a fluorescent whitening agent are added, and LED light source irradiation and ultrasonic treatment are combined; and finally adding sodium hydrogen sulfite and ionic liquid, and completing yellowing prevention through microwave treatment. The bleaching effect is enhanced through cooperation of a nano-composite system and multiple physical fields, pollution of chlorine-containing reagents is avoided, efficient delignification and fiber protection are achieved under the mild condition, and the method is suitable for preparation of high-quality refined cotton.

The present application relates to the field of food processing technology, and discloses a softened kudzu root and its preparation method and application. The preparation method of the softened kudzu root of the present application first places the kudzu root in an acetic acid-sodium acetate buffer solution containing chitosan, and adds lignin peroxidase to perform the first stage enzymolysis; then adds hemicellulase and xylanase to perform the second stage enzymolysis; finally, adds acetic acid to adjust the pH to the optimal pH of cellulase, adds cellulase to perform the third stage enzymolysis, and after enzymolysis, dries to obtain the softened kudzu root. The process of the present application is based on the enzymatic method and is optimized on this basis. It can effectively soften the kudzu root fiber, avoid the problem of kudzu root fiber being difficult to chew, and at the same time retain the toughness and integrity of the kudzu root fiber to the greatest extent, avoid the generation of fiber debris, and can be extended to the production of kudzu root chewing food.

The invention discloses an alkaline high-activity organic water-soluble fertilizer based on coupling of enzymolysis papermaking waste liquid and potassium fulvate and a preparation method of the alkaline high-activity organic water-soluble fertilizer, and relates to the technical field of organic fertilizers. Lignin peroxidase, xylanase and alkaline protease are adopted as a compound enzyme preparation to conduct enzymolysis treatment on concentrated papermaking waste liquid, then enzymolysis activation liquid obtained after enzymolysis is mixed with potassium fulvate, amino acid chelating liquid and a stabilizer, and the alkaline high-activity organic water-soluble fertilizer is prepared through a coupling reaction. Through multi-enzyme system enzymolysis and coupling synergistic reaction, macromolecular harmful organic matters in the papermaking waste liquid are degraded into active small molecules which are easy to absorb, and the active small molecules and the potassium fulvic acid are in synergistic interaction, so that the alkaline high-activity organic water-soluble fertilizer is rich in small-molecule fulvic acid, amino acid and trace elements; therefore, the prepared alkaline high-activity organic water-soluble fertilizer can improve the physical and chemical properties of soil, improve the disease resistance, catalase activity and vitamin C content of tomatoes, and improve the yield and quality of broccoli.

This invention relates to the field of genetic engineering technology, and more particularly to an engineered bacterium with high substrate utilization efficiency, its construction method, and its applications. The method includes overexpressing laccase and lignin peroxidase in two monokaryotic Ganoderma lucidum strains, respectively, and then hybridizing the two monokaryotic strains to form a new Ganoderma lucidum. Ganoderma lingzhi MKLGE253252, depositary institution: China General Microbiological Culture Collection Center; address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; deposit date: March 11, 2026; accession number: CGMCC No. 42594. It synergistically enhances lignin degradation capacity, mycelial utilization efficiency, and growth capacity, and significantly improves the strength of mycelial fiber sheath. This invention fundamentally solves the problems of low lignin utilization efficiency, slow mycelial growth, and insufficient mycelial sheath strength in existing technologies, providing a completely new technical path for the production of bio-based leather.

The invention belongs to the technical field of biological environment-friendly degradable materials, and discloses an environment-friendly biodegradable tissue based on edible fungus mycelia and a preparation method thereof. The tissue takes edible fungus mycelia as a main raw material. The used tissue is placed in a soil environment to be naturally degraded to form a compound. The compound is rich in organic matters and various nutrient elements such as nitrogen, phosphorus and potassium, the content of the nitrogen element can reach 0.23%, and the compound is a high-quality organic fertilizer source. Meanwhile, the compound also contains bioactive enzymes, such as lignin peroxidase, manganese peroxidase, laccase and the like. The biological active enzymes can play a catalytic role to decompose complex organic matters such as lignin and polycyclic aromatic hydrocarbon in the soil, so that more small molecular substances capable of being absorbed and utilized by plants are released. In addition, waste materials can be degraded into fertilizer within 30 days, and closed-loop utilization of resources is achieved.

This invention belongs to the field of microbial application technology, and more specifically, relates to a highly efficient microbial agent for degrading low-density polyethylene and its application. The microbial agent provided by this invention is obtained by inoculating CBS 143848 strain into PDA medium and activating it at 25-30℃ for 25-35 days to obtain an activated bacterial solution. The activated bacterial solution is then inoculated into mineral salt medium at an inoculation rate of 1%-3% (volume fraction) to obtain the microbial agent. The lignin peroxidase and manganese peroxidase in this agent can efficiently degrade low-density polyethylene plastic, achieving a weight loss rate of 4.28±0.0036% within 45 days of cultivation. This microbial agent can be used for plastic degradation in landfills and the environment.

The invention relates to the technical field of microorganisms, in particular to heat-resistant corynebacterium nicotianae and application of the heat-resistant corynebacterium nicotianae in lignocellulose degradation. The invention provides Glutamicobacter nicotianae WT08 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Glutamicobacter nicotianae WT08 is CGMCC No.35741. The invention further provides a preparation method of the Glutamicobacter nicotianae WT08. The corynebacterium nicotianae WT08 can tolerate growth at a high temperature, can generate laccase, lignin peroxidase, cellulase and manganese peroxidase under a high-temperature condition, is a high-temperature-resistant lignocellulose degrading bacterium, can be used for composting treatment of raw materials such as agricultural and forestry wastes, can increase the quantity and activity of microorganisms in a high-temperature period of composting, and can be used for degrading the high-temperature-resistant lignocellulose degrading bacterium. The rapid degradation of the lignocellulose is promoted, and the method has a relatively good application value in the resource utilization of lignocellulose wastes.

The invention belongs to the field of biotechnology and environmental management, and particularly relates to a high-throughput screening transformation and preparation method of efficient lignin peroxidase and a degradation effect of straw lignin. An efficient screening platform is established on the basis of an escherichia coli exocrine system, rapid quantitative detection of the enzyme activity is achieved in combination with an ABTS chromogenic reaction, and the screening efficiency can be improved. Key mutation sites (M52I, A188E and 308I) are determined through a directed evolution technology, the mutant D3 is obtained through combination mutation and a bacillus subtilis surface display technology, the enzyme activity of the mutant D3 is improved by 2.35 times, the degradation efficiency of alkali lignin is improved by 50%, and the mutant D3 is easy to operate and high in stability and economic benefit. The method can be widely applied to efficient degradation of lignin in agricultural wastes (such as straws) or environment-friendly treatment of industrial wastewater (such as papermaking wastewater), and a novel enzyme preparation solution is provided for biomass resource utilization.

The invention relates to the technical field of biomass resource utilization, in particular to a straw pretreatment method and application thereof. The straw pretreatment method comprises the following steps: carrying out steam explosion treatment and composite enzymatic hydrolysis reaction on straws; wherein enzymes for the composite enzymatic hydrolysis reaction comprise lysed polysaccharide monooxygenase, lignin peroxidase and cellobiose dehydrogenase. After the pretreatment, the content of lignin in the straw is obviously reduced, the structural damage of cellulose and hemicellulose is more sufficient, and the breakage degree of glucosidic bonds is improved, so that the efficiency of the subsequent enzymolysis saccharification process and the yield of reducing sugar are greatly improved. The method has the advantages of mild treatment conditions, environmental friendliness, high saccharification efficiency and the like, and is suitable for efficient bioconversion of straw wood fiber raw materials.

The application discloses a low-temperature and drought-resistant straw-degrading bacterium and application thereof. The application screens a low-temperature and drought-resistant straw-degrading bacterium, which is Stenotrophomonas maltophilia (Stenotrophomonas maltophilia) Stenotrophomonas maltophilia ) GF-Y18, has good abilities of producing exo-beta-1, 4-glucanase, laccase and lignin peroxidase, can effectively promote straw decomposition, and has certain low-temperature and drought resistance; the bacterium and the bacterium agent thereof can be used for degrading lignin and / or cellulose and / or straw in a low-temperature and drought environment.

The present invention relates to the fields of gene recombination technology and pharmaceutical preparations, and provides an anticoccidial fusion protein, the amino acid sequence of which is composed of a broad-spectrum antiprotozoal polypeptide APP amino acid sequence, an oxidative stress-sensitive linker amino acid sequence, a flexible linker amino acid sequence, and a lignin peroxidase mutant LiPM amino acid sequence connected in sequence; the amino acid sequences of the broad-spectrum antiprotozoal polypeptide APP, the oxidative stress-sensitive linker, the flexible linker, and the lignin peroxidase mutant LiPM are shown in SEQ ID NOs. 1 to 4, respectively. The present invention also provides a preparation method and application of the above-mentioned anticoccidial fusion protein. The present invention is designed based on APP and LiPM. When used as an insecticide, the outer layer of the oocyst wall can be enzymatically decomposed by LiPM, while APP can enter the inner layer of the eggs to effectively kill the eggs, thereby achieving a better anticoccidial effect.

The application relates to the technical field of degradable materials, and discloses a full-biodegradable bamboo-based cellulose composite modified material and a preparation method thereof, which is composed of the following components: bamboo-based cellulose, modified rosin acid ester, polylactic acid, carboxylated cellulose nanofiber, polybutylene succinate, calcium ion crosslinking agent, bamboo charcoal powder, antioxidant and plasticizer; the modified rosin acid ester is prepared through an ester exchange reaction of rosin acid and polyethylene glycol monomethyl ether; the bamboo-based cellulose is prepared through the following steps of crushing, screening and lignin peroxide enzyme synergistic microwave-ultrasonic pretreatment of bamboo material processing leftovers; through the overall technical scheme of directional activation pretreatment of the bamboo-based cellulose, interface compatibilization of the modified rosin acid ester, carboxylated cellulose nanofiber reinforcement, calcium ion crosslinking network construction and multi-component synergistic compounding, the application realizes systematic optimization, and effectively solves the problems of poor compatibility, low strength, poor thermal stability and uncontrollable degradation of traditional bamboo-based biodegradable materials.

This invention discloses a strain of Bacillus thuringiensis and its application in the degradation of lignocellulose, belonging to the field of microbial technology. The microbial classification name is Bacillus thuringiensis, the strain name is LMU-81, and the accession number is CGMCC NO.36215. Strain LMU-81 was isolated from leaf litter soil and accumulated decaying wood in mangrove wetlands. It exhibits high lignocellulose enzyme activity, particularly lignin peroxidase (LiP), and can be used as a microbial inoculant in combination with other microorganisms to form a compound inoculant for the degradation of lignocellulose in sugarcane bagasse and other materials.

The invention relates to the technical field of resource utilization of agricultural wastes, provides a method for co-producing fibers and fulvic acid from straws at normal temperature, and solves the problems of high energy consumption, heavy pollution and single product in the traditional process. According to the method, multi-stage enzymolysis and stepped mechanical pulping are combined through unique process arrangement, a synergistic effect is achieved, and efficient conversion and near-zero emission of straw resources at the normal temperature are achieved. The method comprises the following specific steps: after straw is crushed and dedusted, carrying out multi-stage enzymolysis and pulping treatment, sequentially degrading lignin and hemicellulose by using a compound enzyme A (laccase / xylanase), a compound enzyme C (compound enzyme A / compound enzyme B) and a compound enzyme B (lignin peroxidase / manganese peroxidase) in stages, refining fibers by combining high, medium and low gradient pulping, and then refining and screening the fibers, so as to obtain the lignin-hemicellulose composite fiber. The fulvic acid solution is recycled, and unqualified fibers are subjected to closed-loop circulation treatment. The method is suitable for various raw materials such as corn straws, cotton stalks and poplar chips, and has the advantages of low energy consumption, environmental protection and economy.

The invention belongs to the technical field of microbial application, and particularly relates to a microbial agent for efficiently degrading low-density polyethylene and application of the microbial agent. According to the microbial inoculum provided by the invention, a CBS 143848 strain is inoculated to a PDA culture medium and activated for 25-35 days at 25-30 DEG C to obtain an activated bacterial solution, and the activated bacterial solution is inoculated to a mineral salt culture medium according to the inoculation amount of 1%-3% of the volume fraction of the mineral salt culture medium to obtain the microbial inoculum. The weight loss rate can reach 4.28 + / -0.0036% within 45 days of culture, and the microbial inoculum can be used for garbage burying and plastic degradation in the environment.

The classification name of the clostridium butyricum GS314 is clostridium butyricum, and the clostridium butyricum GS314 is preserved in the China Center for Type Culture Collection, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, China, the preservation number is CCTCC NO: M 20251824, and the preservation date is August 12, 2025. The activity of endoglucanase synthesized by the clostridium butyricum GS314 is 21.78 U / mL, the activity of filter paper enzyme is 13.37 U / mL, meanwhile, related enzymes for degrading lignin can be synthesized, the enzyme activity of laccase is 6.63 U / mL, the activity of lignin peroxidase is 398.25 U / mL, the activity of manganese peroxidase is 1576.18 U / mL, in addition, the strain can effectively degrade hemicellulose, and in the process of degrading corn straw by using the clostridium butyricum GS314, the yield of corn straw is increased, and the yield of corn straw is increased. Cellulose, hemicellulose and lignin are obviously degraded.

The invention discloses a sugarcane leaf in-situ decomposition microbial agent, which is prepared from the following active ingredients in parts by weight: 25 to 35 parts of bacillus subtilis powder, 10 to 20 parts of aspergillus niger powder, 15 to 25 parts of trichoderma reesei powder and 5 to 15 parts of pseudomonas fluorescens powder; the microbial agent further comprises 40-50 parts of a modified carrier agent and 5-10 parts of an additive agent. According to the decomposition microbial agent, lignin peroxidase and manganese peroxidase secreted by trichoderma reesei in the lignin and cellulose synchronous decomposition microbial agent are adopted, the lignin phenylpropane structure can be destroyed, and the lignin peroxidase and the manganese peroxidase form a synergistic effect with cellulase generated by aspergillus niger.

The invention discloses a white-rot fungus BF-1 capable of degrading high-lignin straws and application thereof, belongs to the technical field of microorganisms, and aims to solve the problem that the feed utilization rate is low due to the fact that internal nutritional ingredients of the high-lignin straws such as wheat straws and rice straws are tightly wrapped by acidic washing lignin. The coriolus versicolor BF-1 is separated and screened from a shiitake mushroom sample, the strain can secrete laccase, manganese peroxidase, lignin peroxidase and other key enzymes, the stubborn lignin structure in straw is effectively decomposed through the synergistic effect, the strain is prepared into a microbial inoculum after being cultured in a suitable culture medium, the microbial inoculum is applied to the preparation process of straw fermented feed, and the straw fermented feed has the advantages of being high in yield and good in application prospect. According to the present invention, the nutritional value, the palatability and the safety of the feed can be improved, the digestion and the absorption of the livestock on the nutrients can be promoted, the green high-value utilization of the straw resource can be achieved, the environmental pollution can be reduced, the sustainable feed source can be provided for the animal husbandry, and the important application prospect and the important promotion value can be provided.

The invention discloses a method for carrying out bacterium-enzyme composite treatment on grape branch compost, and belongs to the technical field of agricultural waste resource utilization. According to the method, a bacterial enzyme composite system is prepared by using own saccharomycetes, lactic acid bacteria strains and lignin peroxidase, during composting, grape pruning waste is crushed, the carbon nitrogen ratio is adjusted, a micro-ecological bacterial enzyme complexing agent is inoculated for composting fermentation, the degradation efficiency of cellulose and hemicellulose is enhanced through the synergistic effect of two bacteria and one enzyme, and the composting period is shortened; the high-quality organic fertilizer is obtained. The invention solves the technical problems of slow degradation of grape pruning waste and long composting period, realizes resource utilization of waste, has the advantages of simple operation, low cost and environmental friendliness, and is suitable for large-scale agricultural production and application.