14 results about "Lignin peroxidase" patented technology

The invention discloses streptomyces pseudogriseogriseus for promoting straw degradation and increasing soil organic matters, and belongs to the technical field of microorganisms. The streptomyces pseudogriseolus NF3 disclosed by the invention has the activity of CMC-Na enzyme, xylanase, laccase, lignin peroxidase and manganese peroxidase, and can be used for degrading cellulose, hemicellulose and lignin at the same time, and the degradation rate of the streptomyces pseudogriseolus NF3 on corn straws reaches 67.75%; the fertilizer is applied to straw returning soil, soil nutrient release is accelerated, the soil organic matter content is increased, and the crop yield is increased.

The invention discloses a production technology of a jute environment-friendly coir mat with a three-dimensional structure, and relates to the technical field of jute coir mat production.The production technology comprises the following steps that S1, raw material pretreatment is conducted, specifically, raw jute is sequentially subjected to three-stage degumming including anionic surfactant solution soaking, pectinase treatment, lignin peroxidase treatment and caustic soda boiling-off, performing countercurrent cleaning and vacuum drying to obtain flexible fibers; s2, mixing fibrils: mixing the pretreated jute fibers with palm fibers and ramie fibers in proportion to form a mixed fiber raw material; s3, opening and carding: performing loosening treatment on the mixed fibers by a bidirectional opener, and preparing flocculent fibers by a carding machine; the flocculent fiber filaments are sprayed to a lapping machine through an exhaust fan, the lapping machine reciprocates at the constant speed of 1.5-2.5 m / s in the direction perpendicular to the spraying direction, the fiber flexibility can be improved, a three-dimensional supporting structure can be constructed, and a formaldehyde adding source can be eliminated.

This invention relates to the field of genetic engineering technology, and more particularly to an engineered bacterium with high substrate utilization efficiency, its construction method, and its applications. The method includes overexpressing laccase and lignin peroxidase in two monokaryotic Ganoderma lucidum strains, respectively, and then hybridizing the two monokaryotic strains to form a new Ganoderma lucidum. Ganoderma lingzhi MKLGE253252, depositary institution: China General Microbiological Culture Collection Center; address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; deposit date: March 11, 2026; accession number: CGMCC No. 42594. It synergistically enhances lignin degradation capacity, mycelial utilization efficiency, and growth capacity, and significantly improves the strength of mycelial fiber sheath. This invention fundamentally solves the problems of low lignin utilization efficiency, slow mycelial growth, and insufficient mycelial sheath strength in existing technologies, providing a completely new technical path for the production of bio-based leather.

The invention belongs to the technical field of biological environment-friendly degradable materials, and discloses an environment-friendly biodegradable tissue based on edible fungus mycelia and a preparation method thereof. The tissue takes edible fungus mycelia as a main raw material. The used tissue is placed in a soil environment to be naturally degraded to form a compound. The compound is rich in organic matters and various nutrient elements such as nitrogen, phosphorus and potassium, the content of the nitrogen element can reach 0.23%, and the compound is a high-quality organic fertilizer source. Meanwhile, the compound also contains bioactive enzymes, such as lignin peroxidase, manganese peroxidase, laccase and the like. The biological active enzymes can play a catalytic role to decompose complex organic matters such as lignin and polycyclic aromatic hydrocarbon in the soil, so that more small molecular substances capable of being absorbed and utilized by plants are released. In addition, waste materials can be degraded into fertilizer within 30 days, and closed-loop utilization of resources is achieved.

This invention belongs to the field of microbial application technology, and more specifically, relates to a highly efficient microbial agent for degrading low-density polyethylene and its application. The microbial agent provided by this invention is obtained by inoculating CBS 143848 strain into PDA medium and activating it at 25-30℃ for 25-35 days to obtain an activated bacterial solution. The activated bacterial solution is then inoculated into mineral salt medium at an inoculation rate of 1%-3% (volume fraction) to obtain the microbial agent. The lignin peroxidase and manganese peroxidase in this agent can efficiently degrade low-density polyethylene plastic, achieving a weight loss rate of 4.28±0.0036% within 45 days of cultivation. This microbial agent can be used for plastic degradation in landfills and the environment.

The invention relates to the technical field of microorganisms, in particular to heat-resistant corynebacterium nicotianae and application of the heat-resistant corynebacterium nicotianae in lignocellulose degradation. The invention provides Glutamicobacter nicotianae WT08 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Glutamicobacter nicotianae WT08 is CGMCC No.35741. The invention further provides a preparation method of the Glutamicobacter nicotianae WT08. The corynebacterium nicotianae WT08 can tolerate growth at a high temperature, can generate laccase, lignin peroxidase, cellulase and manganese peroxidase under a high-temperature condition, is a high-temperature-resistant lignocellulose degrading bacterium, can be used for composting treatment of raw materials such as agricultural and forestry wastes, can increase the quantity and activity of microorganisms in a high-temperature period of composting, and can be used for degrading the high-temperature-resistant lignocellulose degrading bacterium. The rapid degradation of the lignocellulose is promoted, and the method has a relatively good application value in the resource utilization of lignocellulose wastes.

The invention relates to the technical field of biomass resource utilization, in particular to a straw pretreatment method and application thereof. The straw pretreatment method comprises the following steps: carrying out steam explosion treatment and composite enzymatic hydrolysis reaction on straws; wherein enzymes for the composite enzymatic hydrolysis reaction comprise lysed polysaccharide monooxygenase, lignin peroxidase and cellobiose dehydrogenase. After the pretreatment, the content of lignin in the straw is obviously reduced, the structural damage of cellulose and hemicellulose is more sufficient, and the breakage degree of glucosidic bonds is improved, so that the efficiency of the subsequent enzymolysis saccharification process and the yield of reducing sugar are greatly improved. The method has the advantages of mild treatment conditions, environmental friendliness, high saccharification efficiency and the like, and is suitable for efficient bioconversion of straw wood fiber raw materials.

This invention discloses a strain of Bacillus thuringiensis and its application in the degradation of lignocellulose, belonging to the field of microbial technology. The microbial classification name is Bacillus thuringiensis, the strain name is LMU-81, and the accession number is CGMCC NO.36215. Strain LMU-81 was isolated from leaf litter soil and accumulated decaying wood in mangrove wetlands. It exhibits high lignocellulose enzyme activity, particularly lignin peroxidase (LiP), and can be used as a microbial inoculant in combination with other microorganisms to form a compound inoculant for the degradation of lignocellulose in sugarcane bagasse and other materials.

The invention belongs to the technical field of microbial application, and particularly relates to a microbial agent for efficiently degrading low-density polyethylene and application of the microbial agent. According to the microbial inoculum provided by the invention, a CBS 143848 strain is inoculated to a PDA culture medium and activated for 25-35 days at 25-30 DEG C to obtain an activated bacterial solution, and the activated bacterial solution is inoculated to a mineral salt culture medium according to the inoculation amount of 1%-3% of the volume fraction of the mineral salt culture medium to obtain the microbial inoculum. The weight loss rate can reach 4.28 + / -0.0036% within 45 days of culture, and the microbial inoculum can be used for garbage burying and plastic degradation in the environment.

The invention discloses a sugarcane leaf in-situ decomposition microbial agent, which is prepared from the following active ingredients in parts by weight: 25 to 35 parts of bacillus subtilis powder, 10 to 20 parts of aspergillus niger powder, 15 to 25 parts of trichoderma reesei powder and 5 to 15 parts of pseudomonas fluorescens powder; the microbial agent further comprises 40-50 parts of a modified carrier agent and 5-10 parts of an additive agent. According to the decomposition microbial agent, lignin peroxidase and manganese peroxidase secreted by trichoderma reesei in the lignin and cellulose synchronous decomposition microbial agent are adopted, the lignin phenylpropane structure can be destroyed, and the lignin peroxidase and the manganese peroxidase form a synergistic effect with cellulase generated by aspergillus niger.

The invention discloses a white-rot fungus BF-1 capable of degrading high-lignin straws and application thereof, belongs to the technical field of microorganisms, and aims to solve the problem that the feed utilization rate is low due to the fact that internal nutritional ingredients of the high-lignin straws such as wheat straws and rice straws are tightly wrapped by acidic washing lignin. The coriolus versicolor BF-1 is separated and screened from a shiitake mushroom sample, the strain can secrete laccase, manganese peroxidase, lignin peroxidase and other key enzymes, the stubborn lignin structure in straw is effectively decomposed through the synergistic effect, the strain is prepared into a microbial inoculum after being cultured in a suitable culture medium, the microbial inoculum is applied to the preparation process of straw fermented feed, and the straw fermented feed has the advantages of being high in yield and good in application prospect. According to the present invention, the nutritional value, the palatability and the safety of the feed can be improved, the digestion and the absorption of the livestock on the nutrients can be promoted, the green high-value utilization of the straw resource can be achieved, the environmental pollution can be reduced, the sustainable feed source can be provided for the animal husbandry, and the important application prospect and the important promotion value can be provided.

The invention discloses a method for carrying out bacterium-enzyme composite treatment on grape branch compost, and belongs to the technical field of agricultural waste resource utilization. According to the method, a bacterial enzyme composite system is prepared by using own saccharomycetes, lactic acid bacteria strains and lignin peroxidase, during composting, grape pruning waste is crushed, the carbon nitrogen ratio is adjusted, a micro-ecological bacterial enzyme complexing agent is inoculated for composting fermentation, the degradation efficiency of cellulose and hemicellulose is enhanced through the synergistic effect of two bacteria and one enzyme, and the composting period is shortened; the high-quality organic fertilizer is obtained. The invention solves the technical problems of slow degradation of grape pruning waste and long composting period, realizes resource utilization of waste, has the advantages of simple operation, low cost and environmental friendliness, and is suitable for large-scale agricultural production and application.