116 results about "Lignin peroxidase" patented technology

The enzymatic straw material pulping process includes three stages of mechanical pre-treatment, enzyme treatment and chemical pulping or chemical-mechanical pulping. After being pretreated mechanically in a pulp grinder, the straw material is treated with hemicellulase liquid or mixed enzyme liquid comprising hemicellulase and lignin peroxidase at pH 3.5-6.5 and 40-60 deg.c for 1-10 hr. In chemical pulping, the material is presoaked with waste pulp-making liquor before extruding out wastes liquor and digested; and in chemical-mechanical pulping, the material is first treated with EDTA, NaOH and H2O2 at 40-80 deg.c for 30-120 min and then ground to form the pulp. The present invention is used in papermaking enterprises with straw as material and has the advantages of low pollution load, high, paper pulp quality, easy-to-process waste liquor, etc.

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

The invention discloses a Myrothecium verrucaria mutant strain T2901 with a collection number of CCTCC NO: M2017413. The strain T2901 obtained by separation and mutagenesis can efficiently degrade lignin in corn straw. The strain T2901 can secrete laccase, lignin peroxidase and manganese peroxidase related to lignin degradation. Studies on impact of microbial pretreatment on structure and composition of the corn straw show that the strain T2901 has no loss of saccharides like cellulose and hemicelluloses, cellulose is retained greatly, and a new path for increasing straw cellulose enzymolysisutilization rate is provided.

The invention provides a method for pretreating cotton fabrics by adopting glucoamylase/glucose oxidase, pectinase and CBD (cellulose-binding domain)-lignin peroxidase, belonging to the technical field of cotton fabric dyeing and finishing pretreatment. The method is characterized in that the cotton fabrics are firstly treated in mixed enzyme solution containing the glucoamylase and the glucose oxidase and the glucoamylase catalyzes the starch size on the fabrics to be decomposed into glucose so as to eliminate the size; the glucose oxidase catalyzes the glucose and oxygen to act to generate hydrogen peroxide; the generated hydrogen peroxide not only plays a role of bleaching but also can damage the hydrophobic surface structure of the cotton to expose the lower pectic substances; then the cotton fabrics are treated in alkaline pectinase solution to remove the pectic substances through decomposition; and finally the cotton fabrics are treated in CBD-lignin peroxidase solution to finally remove the cotton seed hulls through decomposing the lignin in the cotton seed hulls. The method provided by the invention can replace the traditional chemical treatment method to realize full bio-enzyme pretreatment of the cotton fabrics, and at the same time the traditional hydrogen peroxide can be no longer adopted for bleaching.

The invention relates to an organic fertilizer and a production method thereof, and particularly relates to a method for producing an organic fertilizer by utilizing hickory hulls. The method comprises the following steps of: uniformly mixing hull powder, meal and superphosphate, inoculating the obtained mixture into a microbial inoculan I after moisture is adjusted, and carrying out aerobic fermentation under the turning condition; after fermentation, inoculating a microbial inoculan II into the material, and then carrying out piled aging, drying, crushing, sieving and packaging and the like to obtain a hickory hull organic fertilizer. Aiming at the characteristics that lignin in the hull has a high content, and is difficult to break down, trametes gallic which has strong adaptability and is capable of breaking down lignin and cellulose is added during the piled aging, paint enzymes and lignin peroxidases generated in the growth process of the trametes gallic can promote conversion of lignin to humic acid, and simultaneously, lignin wrapped cellulose is released; and after the cellulose is treated at high temperature in the aerobic fermentation process, the cellulose is changed in structure, so that the cellulose is easily utilized by microbes, the material is composted more thoroughly, and the content of humic acid in the product is increased.

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

A process for mechanically preparing the alkaline hydrogen peroxide pulp from raw straw materials includes such steps as immersing raw straw materials in hot water, squeezing for dewatering, chemical pretreating, grinding, enzyme treating with the solution of semi-cellulase or the solution of semi-cellulase and lignin peroxidase, chemical pretreating, and grinding. The resultant pulp features high whiteness and strength and easy treatment of its sewage.

The invention provides microorganism compound microbial inoculums capable of efficiently degrading straw lignin. Floras achieve mutualistic symbiosis and synergistic interaction. In a straw fermentation process, three enzymes relating to the degradation of the lignin can be secreted at the same time; the activities of laccase, lignin peroxidase and manganese peroxidase are respectively 23.56 U/g,23.02 U/g and 21.74 U/g biomass; the lignin content of the fermented corn straws is reduced by 60.21 percent, and loss of sugar such as celluloses and hemicelluloses is avoided; the conversion rate ofthe celluloses in the pretreated corn straws is greater than that of non-treated corn straws by 142.14 percent; a screened compound bacterial strain is high in lignin degradation capacity, and the industrial utilization efficiency of the straws pretreated by the bacterial strain is obviously improved. The research provides a new pretreatment way for enzymolysis saccharification of lignocellulosesin the straws.

The invention provides a biological pulping method adopting crop straw and papermaking pulp and belongs to the technical field of pulping. The biological pulping method comprises crop straw cutting, primary treatment with bio-enzyme, grinding, secondary treatment with a microbial agent and bleaching. During primary treatment of with the bio-enzyme, an adopted complex bio-enzyme preparation comprises lignin peroxidase, xylanase, laccase, pectinase and pulping enzyme in the mass ratio being (2-4):(1-2):(1-2):(1-2):(1-2), and the technical problem that traditional pulping methods exert enormous pressure on the environment is relieved. By means of the biological pulping method adopting crop straw, emission of wastewater and waste gas is reduced, energy consumption is reduced, the pulp yield and the whiteness are increased, the crop straw can be changed into wealth, and the method has great significance in energy conservation, emission reduction and low-carbon economy.

The invention relates to a white rot fungi continuous high-efficient enzyme producing method, which belongs to the environmental biotechnical field and can be applied in the condition of difficult degradation of organic waste water treatment and environmental repair. In order to realize the aims of the invention, the white rot fungi continuous high-efficient enzyme producing method uses cobs as growth carriers and supplemental nutrition substrates of white rot fungus, utilizes an air-lift fermentation apparatus and optimizes the fermentation conditions, realizes continuous high-efficient output of lignin peroxidases and manganese peroxidases, and simultaneously reduces the enzyme production cost.

The invention discloses a lignin-degrading enzyme regulator and an application thereof. The lignin-degrading enzyme regulator is added by two steps in an aerobic composting process. The lignin-degrading enzyme regulator comprises a manganese peroxidase and lignin peroxidase regulator and a laccase regulator, wherein the manganese peroxidase and lignin peroxidase regulator comprises mixed liquid A of Tween 80, hydrogen peroxide and trace stimulation elements; the laccase regulator comprises mixed liquid B of Tween 80, 1,2-dimethoxybenzene and trace stimulation elements. The lignin-degrading enzyme regulator can be used for regulating secretion of lignin-degrading enzyme in fungi and has the advantages of good promotion effect, simple, economic and practical technology and the like.

The invention belongs to an agricultural waste recycling technology and discloses a degrading fungus capable of decomposing trimmed pear branches and a fungicide of the degrading fungus. The strain D10 belongs to Ceriporia lacerata which is preserved with the number of CGMCC NO.7852 in China general microbiological culture collection center on July 8th, 2013. The strain can grow on a culture medium with a pear branch as the unique carbon source. The maximum enzyme activity values of laccase, lignin peroxidase and manganese peroxidase of the strain D10 are respectively 11.03U.Ml<-1>, 36.71U.Ml<-1>and 152.75U.Ml<-1> in the 21d liquid fermentation process. According to the invention, agricultural waste trimmed pear branches are degraded by using a microbial fermentation method and are converted into an organic fertilizer, so that wastes are turned into wealth, the environment is protected, and the application prospect is wide.

The invention provides a method for manufacturing fried bamboo shoots. The method comprises the following steps: (S1) cutting bamboo shoots into strips or sheets and cooking; (S2) cooling the cooked bamboo shoots in the step S1 in cold water; (S3) adding lignin peroxidase into the cooled bamboo shoots for performing enzymolysis; (S4) adding auxiliary materials into the bamboo shoots subjected to enzymolysis in the step S3; and (S5) frying and taking out the bamboo shoots mixed with the auxiliary materials, and obtaining the fried bamboo shoots. According to the method for manufacturing fried bamboo shoots, the bamboo shoots are subjected to boiling and lignin degradation treatment, so that the bamboo shoots are not hardened in a subsequent frying process, and the bamboo shoots can further form fried foods.

The invention discloses a fungus with straw degradation capability and application thereof in composting and relates to the technical field of biological environmental protection. The straw degradation fungus JSD-17 is penicillium oxalicum (Penicillium oxalicum) and has a preservation number of CGMCC No.7743; the straw degradation fungus is further used for fermenting and composting agricultural waste straw. The penicillium oxalicum disclosed by the invention not only has strong cellulose enzyme synthesis capability, but also has the capability of decomposing hemicellulose and pectin components in the straw. By adopting the fungus disclosed by the invention, laccase (Lac), lignin peroxidase (Lip) and manganese peroxidase (Mnp) can be synthesized; the degradation speed of lignin components in the straw can be greatly increased.

The invention discloses Chinese yew pulp and a preparation method thereof. Chinese yew stalks are used as raw materials, shredded by a rolling and grinding shredding machine and then added into the potassium hydroxide solution for pre-impregnation, water is squeezed out after pre-impregnation, complex enzyme comprising lignin peroxidase and manganese peroxidase according to the mass ratio of 5:1-4 is added into the solution, the Chinese yew stalks are soaked for 30-40h at the temperature of 35-50 DEG C, alkali cooking is performed after defibrination, total chlorine free bleaching is performed after shoving, cleaning, screening and impurity removal, and then paper is manufactured with pulp after acid treatment, washing and desanding, so that the Chinese yew pulp is prepared. A using approach with a high added value is provided for the Chinese yew stalks, the application field of the Chinese yew stalks is widened, a low-pollution mature production process is provided for preparing dissolving pulp and regenerated cellulose fibers with the Chinese yew stalks, and shortage of regenerated cellulose fiber raw materials at present is relieved.

The invention relates to an engineering bacterium based on lignin peroxidase and an implementation method of the engineering bacterium. The implementation method comprises the following steps: with DNA of a streptomyces griseorubens genome as a template, carrying out PCR amplification on the DNA of the streptomyces griseorubens genome and a primer containing a restriction site and a glutathione label to obtain a nucleotide sequence for coding the lignin peroxidase; and then connecting the gene sequence obtained by virtue of amplification to an expression vector, and then transferring an obtained ligation product to an escherichia coli expression strain to obtain a lignin peroxidase expression recombination strain. With the adoption of the implementation method, by virtue of methods of constructing a heterogeneous expression vector, optimizing conditions and fusion tags of protein inducing expression and the like, extracellular over expression of the lignin peroxidase is realized.

The invention discloses a complex microbial agent for degrading straw, a preparation method of the complex microbial agent and a method for degrading the straw. The complex microbial agent for degrading the straw is obtained through mixed culture of lysinibacillus macroides and paenibacillus barcinonensis. Pectinase, cellulase, manganese peroxidase, lignin peroxidase and the like, which are relatively high in activity, can be generated; on the basis of enzyme system components which are on a relatively high amount and cause a coordinated effect on the straw, such components as pectic substances, cellulose, hemicellulose, lignin and the like can be degraded, so that rapid and full degradation of the straw is achieved.

The invention discloses a method for producing ligninolytic enzymes, and belongs to the biotechnical field. The method comprises the following steps: 1, respectively cloning lignin peroxidase, manganese peroxidase and laccase genes from microbes; 2, constructing a Pichia pastoris expression vector containing the expression cassettes of the above three genes, and a Saccharomyces cerevisiae expression vector containing the expression cassettes, and converting the above vectors to corresponding host bacteria to obtain engineering bacteria; and 3, respectively fermenting Pichia pastoris expression engineering bacteria and Saccharomyces cerevisiae engineering bacteria to produce recombinant lignin peroxidase, manganese peroxidase and laccase. The method has the following advantages: engineering bacteria are used to substitute natural bacterial strains to produce the lignin peroxidase, manganese peroxidase and laccase, so the enzyme outputs are improved through increasing the copy numbers of the genes; and the engineering bacteria containing three enzyme gene expression cassettes substitute engineering bacteria containing a single enzyme gene expression cassette to produce single enzymes, so the raw material, the energy consumption and the manpower resource are saved.

The invention discloses a preparation method of xylanase or lignin peroxidase, which comprises the following steps: (1) activating and culturing green Saccharomonospora strain to prepare mother liquor; (2) inoculating the mother liquid obtained in step (1) into Sauton culture medium for inducing to produce enzyme which contains induction substrate for culture; (3) centrifuging the bacteria liquid obtained in step (2) and then collecting clear liquid to obtain the crude extract of the enzyme; and (4) dialyzing the crude extract in step (3), and then carrying out chromatography and purification to obtain theenzyme solution of the xylanase or the lignin peroxidase. The mother liquid obtained in step (1) can be also inoculated into the lignin peroxidase containing the induction substrate prepared by post-fermentation and culture. The xylanase or the lignin peroxidase prepared by the method of the invention has lower cellulase activity, thereby preventing reduction of fiber strength and viscosity of thick liquid in the process of enzyme processing; and the prepared xylanase or the lignin peroxidase has certain heat-resistance and alkali resistance, can directly and effectively attack lignin and degrade hemicellulose, is applicable to pre-bleaching paper pulp in industrial production, and has excellent bleaching effect.

The invention discloses a preparation method and application of immobilized lignin peroxidase. The preparation method comprises the following steps of (1) preparing ferroferric oxide nano-particles through ferric trichloride, sodium citrate and sodium acetate; (2) preparing magnetic silicon dioxide nano-particles; (3) preparing dopamine polymerization embedded magnetic silicon dioxide nano-particles; and (4) preparing magnetic silicon dioxide immobilized lignin peroxidase through dopamine polymerization in-situ embedding. The immobilized lignin peroxidase prepared with the method and hydrogenperoxide are added in wastewater containing organic pollutants, and reaction is performed at room temperature to remove the organic pollutants. A preparation technology is simple and convenient; an adopted immobilized carrier is nanometer sized particles, is safe without toxicity, is high in stability, large in specific surface area, high in binding rate with enzyme, relatively good in dispersibility in a catalytic system and higher in removal efficiency to the pollutants without the risk of secondary pollution and can recycle immobilized enzyme through magnetic separation and recovery.

The invention discloses a method for preparing rice hull ash active carbon by enzymatic pretreatment of rice hull. The method comprises the steps of: firstly, building a lignin degradation enzyme system with a certain enzyme activity ratio to degrade rice hull, wherein the lignin degradation enzyme system is composed of lignin peroxidase, manganese peroxidase and laccase, then degrading the rice hull by a cellulose degradation enzyme system with a certain enzyme activity ratio, wherein the cellulose degradation enzyme system is composed of endoglucanase, exoglucanase and beta-glucosaccharase, and finally distilling to prepare the rice hull ash. The rice hull is subjected to pretreatment by the lignin degradation enzyme system and the cellulose degradation enzyme system; the surface micro-pore structure of the rich hull active carbon is improved; and the adsorptive property is good.

The invention discloses a bacterium L2 used for degrading pear branches, with a classification name of Bacillus megaterium, preserved in China General Microbiological Culture Collection Center on 8th, July, 2013, with a culture preservation number of CGMCC NO.7899. The bacterium L2 can grow on a culture medium using analogue of lignin, guaiacol as a unique carbon source or pear branch powder as the unique carbon source, and can generate a fading ring on an LB-aniline blue plate. Experiments show that after culture suspension of the acterium L2 with an inoculation amount of 1% is inoculated on the culture medium, and solid state fermentation is performed for 30d, a weight loss ratio of the culture medium can reach 105. During a liquid state fermentation process on the 21st day, the highest value of activity of generated lignin peroxidase is 21.76U*mL-1, and the highest value of activity of generated manganese peroxidase is 106.36U*mL-1.

This invention relates to an electrochemistry measurement of the peroxid enzyme activity, the method uses the auricome and eldopaque as the enzyme base and measures the activity of the hyperoxide in the dunghill lixivium with xanthan even bacterium and establishes the linear relation between the current change slope and the enzyme activity. The relation is as following: its peroxide enzyme is 2.27í½29.79U/L and manganese peroxide is 0.085í½1.37U/L.