699 results about "Clostridium butyricum" patented technology

The invention discloses a special gestation compound feed for sows at later stage of pregnancy. The special gestation compound feed is prepared from the following raw materials: corn, bran, rice bran meal, DDGS (dried distiller grains with soluble), swelling soya dregs, swelling soybean flour, fermented soybean meal, compound sugar, mountain flour, calcium hydrogen phosphate, choline chloride, common salt, compound premix, L-lysine hydrochloride, L-threonine, acidifier, clostridium butyricum and yeast cell wall polysaccharides. The invention, starts from nutrition of the sows at the later stage of the pregnancy, gives consideration to the growth of fetuses and the physiological characteristics of the sows per se, designs daily ration with high protein, high amino acid and low energy, adds the compound sugar constituted by different monosaccharides and disaccharides, and can provide energy which can be utilized quickly and improve the birth weight and the uniformity of piglets; and by adding the clostridium butyricum and the yeast cell wall polysaccharides, the special gestation compound feed can improve the intestinal health of the sows, improve immunity, regulate intestinal peristalsis, reduce the constipation incidence of the sows, shorten the labor stage of the sows, reduce the incidence rate of mastitis and endometritis of the sows, and assist the sows in rapid postpartum recovery.

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.

The invention discloses a manufacturing method of butyrate spindle bacillus microecological agent, which comprises the following steps: preparing culture medium; dissolving the culture medium; adjusting pH value; loading in the ferment tank; heating to sterilize the culture medium in the ferment tank; inoculating the butyrate spindle bacillus seed; controlling temperature to ferment; detecting the bacteria number of ferment liquid; adsorbing ferment liquid through plant fiber powder; drying; grinding; obtaining the powder agent.

The invention discloses a method for preparing a bacillus subtilis and clostridium butyricum composite bacterial preparation, which is characterized by comprising the following steps: (1) preparing seed culture solution, namely preparing a seed solution containing clostridium butyricum and a seed solution containing bacillus subtilis respectively, wherein the concentration of the bacterial solution is 0.5*10<8> to 1.0*10<8>CFU/ml; (2) preparing a coculture medium; (3) adding the seed solution of the bacillus subtilis into the coculture medium prepared by the step (1) according to a ratio of 2 percent, automatically regulating the pH value to 6.5 to 7.0 by using 10-percent diluted hydrochloric acid or 10-percent sodium hydroxide, introducing gas, stirring, controlling dissolve oxygen content to be 20 to 50 percent, culturing for 18 to 20 hours at 37 DEG C, stopping introducing gas, and continuing to stir and culture for 2 to 4 hours; and (4) adding the seed solution of the clostridium butyricum prepared by the step (1) into the coculutre medium by using a peristaltic pump according a ratio of 2 percent, standing at 35 DEG C and culturing for 24 to 28 hours to obtain the bacillus subtilis and clostridium butyricum composite bacterial preparation. In the invention, a microecological preparation is mixed with feed according to a ratio of 0.5 percent, the feed is directly used to feed livestock, the diarrhea rate and death rate of the livestock are lowered effectively, yield is increased, and medicine administration cost is saved.

The invention discloses a clostridium butyricum having a diarrhea treating effect. The strain is named as clostridium butyricum Cb-2 which is conserved in China Center for Type Culture Collection with the conservation number of CCTCC M2011384, in November 9th, 2011. A test proves that the clostridium butyricum disclosed by the invention has strong acid producing capability especially butyric acidproducing capability; and the clostridium butyricum has obvious effects on diseases, such as intestinal tract flora infection, acute and chronic diarrhea, irritable bowel syndrome and non-ulcer dyspepsia, caused by various reasons, and has no toxicity and harm. Thus, the clostridium butyricum or clostridium butyricum powder disclosed by the invention is used as a raw material for preparing a micro-ecological preparation and used for replacing sodium butyrate to be applied; and when a specific application is carried out, a clostridium butyricum preparation can be singly used as an active component to be prepared into the micro-ecological preparation together with corn starch, or, the clostridium butyricum preparation is mixed with at least one of other bacterial preparations and the corn starch to prepare a composite micro-ecological preparation.

The invention relates to the technical field of feed processing and specifically provides a microbial fermented feed and a production process thereof. The microbial fermented feed is composed of 60% by mass of waste stevia rebaudiana residue, 15% by mass of corn flour, 15% by mass of soybean meal and 10% by mass of liquid strains, wherein the liquid strains comprise lactobacillus fermenti, saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. According to the invention, a plurality of microbes are utilized and scientifically collocated for fermentation of processing waste of stevia rebaudiana, and the microbial fermented feed with the advantages of low cost, a high nutritional value, fragrant flavor and agreeable taste is produced.

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

The invention provides feed for improving the digestive and immunologic ability of chicken, which comprises a feed carrier, choline chloride, lysine hydrochloride, phenylalanine dihydrochloride, pantothenic acid, clostridium butyricum, lactobacillus plantarum, sodium butyrate, stone powder, folic acid, riboflavin, vitamin E, vitamin K, glucolase, amylase, sodium selenate, magnesium sulfate and a Chinese herbal medicine composition additive. The feed has the beneficial effect that a digestion system and an immune system of the chicken can be improved, so that the feeding management and epidemic prevention works during the brooding period are better improved.

The present invention discloses a feed micro-ecological preparation, an anti-nutritional substance-free feed and a preparation method thereof, and belongs to the technical field of feed. The feed micro-ecological preparation is prepared by fermenting the following raw materials in parts by weight: 1.0-1.2 parts of Saccharomyces cerevisiae, 0.2-0.6 part of Enterococcus faecalis, 1.5-2.0 parts of Bacillus subtilis, 0.2-0.6 part of Bacillus coagulans, 0.1-0.15 part of Bacillus licheniformis, 0.1-0.3 part of Bacillus lentus, 0.2-0.5 part of Clostridium butyricum and 993.05-995.15 parts of liquid medium. After the feed micro-ecological preparation is mixed with feed raw materials, enzymes produced in the process of microbial strain fermentation can decompose anti-nutritional substances in feed and increase the feed utilization rate; at the same time, microbial strains also have effects in improving animal intestinal environment, maintaining intestinal flora balance, inhibiting the growth of pathogenic bacteria, and improving animal immunity.

The invention discloses microbial fermentation feed as well as a production method and an application thereof. The production method of the microbial fermentation feed comprises the following steps: (1) lactic acid bacteria, Clostridium butyricum Cb-2, yeast, Bacillus subtilis, a fermentation base-material and water are uniformly mixed, and a fermentation raw material is obtained; 2) aerobic fermentation and anaerobic fermentation are carried out in order for the fermentation raw material obtained in the step (1), and after fermentation, the feed is obtained. The microbial fermentation feed contains microecological viable organisms, and the total amount of beneficial bacteria is more than 5 x 10<9> cfu/g; the amount of mould and the content of mould toxin are lower than national limit standards; the feed is rich in organic acid, the content of lactic acid reaches 2.5%, and pH value is lower than 4.5; the titer of antibacterial peptide is higher than 1000 u/g, the titer of neutral protease is 150 u/g, the titer of alpha-amylase 300 u/g, dry weight for above, and the titer of total protease is higher than 600 u/g; the whole fermentation process is controlled in the range of 8-24 hours, the fermentation time is saved, and the dissipating energy is controlled below 3%.

The invention discloses a biological antimicrobial-free feed additive and a preparation method thereof, and belongs to the field of animal food. The biological antimicrobial-free feed additive mainly comprises the following components in parts by weight: 28-35 parts of yeast cell wall polysaccharide, 23-30 parts of bacillus licheniformis, 28-45 parts of bacillus subtilis, 17-19 parts of lactobacillus acidophilus, 17-19 parts of clostridium butyricum, 5-10 parts of selenium yeast, 60-70 parts of a coated slow-release acidifying agent, 150-160 parts of wheat bran, 120-150 parts of stone powder and a complex enzyme preparation, wherein the complex enzyme preparation comprises the following raw materials in parts by weight: 7-10 parts of phytase, 7-10 parts of glucanase, 10-15 parts of zytase and 7-10 parts of cellulase. The feed additive provided by the invention can produce various nutrient substances such as beneficial bacteria, complex enzyme and yeast peptides as being subjected to three fermentation processes of liquid tertiary fermentation, solid fermentation and biological feed fermentation, promotes the growth of intestinal villi, enhances the disease-resistant and disease-preventing abilities, and is mainly used for biological anti-free feed.

The invention aims to provide a Clostridium butyricum GK13 bacterial strain CCTCCNO. M2010175. The bacterial strain has the characteristics of high caproic acid yield and good growth stability, can be applied in preparing high-concentration compound Clostridium butyricum liquid after strengthening functional bacteria, can be applied in cultivating artificial pit mud, obtains the effect of shortening the newly-built cellar pool aging period and rejuvenating the degrade cellar pool function in the practical production, improves the alcohol yield and the superior product rate, and solves the problems of rejuvenating a degrade old cellar pool, and shortening the newly-built cellar pool aging time and the like.

The invention relates to a feed additive and a preparation method thereof, in particular to a traditional Chinese medicine microecological preparation and a preparation method thereof. The traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp comprises the following components in percentage by weight: 25-35% of bacillus subtilis, 30-40% of bacillus aceticus and 20-35% of clostridium butyricum. In the invention, the traditional Chinese medicine extracting solution is used for culturing microbes, and the cultured microecological preparationcontains rich polysaccharide components, probiotics and metabolite thereof of acalypha australis, polygonum bydropiper L., purslane and the like. The compound traditional Chinese medicine microecological preparation as the feed additive can be used as a substitute for antibiotic, and the additive prepared into the feed of the grass carp is green and safe, can prevent and treat the enteritis of the grass carp, enhances the immunity and disease resistance, can improve the conversion rate of the feed, and is favorable for purifying water and protecting and improving the ecological environment.

The invention discloses a probiotic and prebiotic composition for forming lean body mass and application thereof, and relates to the field of biomedicine. The composition comprises probiotics such asbifidobacterium lactis, bifidobacterium longum, lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus acidophilus, streptococcus thermophilus, bacillus coagulans and clostridium butyricum, anda prebiotic mixture is also added. By reasonably matching the components of the composition, the intestinal flora is effectively regulated, and the obesity is more efficiently and healthily relieved;for people with normal weight, the weight reduction effect is not obvious, but the body shape is more beautiful; meanwhile, the composition can effectively prevent hyperglycemia, hyperlipidemia and other diseases and improve the body immunity, is suitable for a wider range of people, and can be applied to food, pharmacy and healthy or natural product production.

The invention relates to a clear liquid fermentation medium for clostridium butyricum. The clear liquid fermentation medium, the pH value of which is 7-8, is made from glucose, tryptone, a yeast extract powder, ammonium sulfate, sodium bicarbonate, a maize liquid powder and the like. The clear liquid fermentation medium for clostridium butyricum contains metal salts for promoting the growth of gemma, wherein the metal salts are manganese sulfate, magnesium sulfate and ferrous sulphate. The enrichment medium of the clear liquid fermentation medium for clostridium butyricum contains yeast extract, beef extract, tryptone, glucose, soluble starch, sodium chloride, sodium acetate trihydrate, cysteine hydrochloride, methylene blue at the concentration of 0.5% and distilled water; and the pH is adjusted to 7.1. A fermentation culture method of the clear liquid fermentation medium for clostridium butyricum comprises the following steps of: glycerin tube refrigerated strain activation, heating and optimization, Erlenmeyer flask first-order seed culture, liquid fermentation and spray drying. The production of clostridium butyricum has advantages of simple operation, high amount of zymocyte, high gemma yield, simple post-treatment, less impurities in a bacterial powder sample and high amount of effective live bacteria, and saves cost at large.

The invention discloses a method for preparing a spirit flavoring spirit or a spirit flavoring liquor mainly aroma through liquid state fermentation and biological esterificaiton by using wastes produced from solid fermentation spirit production as culture mediums of composite bacteria. The composite bacteria comprise clostridium butyricum with a bacteria number of SICC1. 91, clostridium koseri with a bacteria number of SICC1. 13 and clostridium barati with a bacteria number of SICC1. 2; the composite bacteria fermentation mediums comprise yellow water, tail water, lees solution and pot-bottom water with a weight ratio of 7-8: 0. 5-1: 0. 5-1: 0. 5-1. Sodium acetate 1. 5and dibasic potassium phosphate 0. 04are added in the mixture solution, and ammonium bicarbonate is added to adjust pH value to 6. 5-7. 0, 95 degree ethanol 2 0s added after sterilization, then the mixture solution is sterilized at temperature 105 DEG C for 20min. The invention solves the pollution problem of the wastes to the water environment from the solid spirit production process. The added spirit body is more coordinate, which prevents the defect that the addition of monomer product easily generates float fragrant flavor. The spirit has strong nature feeling, and the recovered bacterial sludge and other residual nutritive matters can be recycled in the fermentation process and avoid secondary environment pollution.

The invention discloses a strain called acid-producing bacillus cereus, which is collected in the China center for type culture collection, with the preservation number of CCTCC M 2011352, on October 16th, 2011. The strain can be used for preparing micro-ecological preparations. During application, acid-producing bacillus cereus powder can be used as an individual effective component to be mixed with corn starch to prepare the micro-ecological preparations, or is mixed with at least one other effective component or strain powder and corn starch to prepare composite micro-ecological preparations. The invention further provides a composite micro-ecological preparation M3 which is prepared by three high-temperature-resistant strains including bacillus subtilis N9-1-35, clostridium butyricum Cb-2, the acid-producing bacillus cereus and corn starch according to the mass ratio of 1:2:2:5, wherein the viable counts of the strain powder are larger than or equal to 1.0X109cfu/g, 5.0X108cfu/g and 5.0X108cfu/g respectively.

The present invention discloses a synbiotic feed additive, and a preparation method and applications thereof. The synbiotic feed additive comprises: 1 ~ 5 parts by weight of, preferably 2-4 parts by weight of, more preferably 3-4 parts by weight of, and most preferably 3.5 parts by weight of at least one selected from chitosan oligosaccharide, chitosan and algal oligosaccharide; 2-10 parts by weight of, preferably 3 to 9 parts by weight of, more preferably 4-8 parts by weight of, and most preferably 5.5 parts by weight of at least one selected from astragalus polysacharide, and lycium barbarum polysaccharides hawthorn flavone; and 100 parts by weight of probiotics composition which is composed of bacillus subtilis, bacillus coagulans and clostridium butyricum at a weight ratio of 1-3:1-3:1-3, preferably 3:3:3, relatively preferably 3:2:2, more preferably 3: 2:1, and most preferably 3:1:1. Compared with existing synbiotic feed additives, the synbiotic feed additive of the present invention is easier to digest and absorb, and can significantly improve animal immunity and animal health, increase feed utilization, lower feed conversion rates, promote reproduction, improve production performance, improve meat quality and improve breeding environment.

The invention relates to a solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans, wherein a production method is a solid state fermental cultivation method of the mixed bacteria. A solid state fermentation culture medium is formed with enzymolysis bean pulp and rice bran as major ingredients and added with proper amount of an inorganic salt; the solid state fermentation culture medium is subpackaged and then sterilized; next, the solid state fermentation culture medium is inoculated with an isometric clostridium butyricum TK2 seed solution accounting for 10% of the total weight of the solid state culture medium and an isometric bacillus coagulans TQ33 seed solution accounting for 5% of the total weight of the solid state culture medium, and stirred evenly for fermentation. After the fermentation is carried out at 37 DEG C for 48 hours, a compound probiotics preparation of the clostridium butyricum TK2 and the bacillus coagulans TQ33 is obtained by drying at 50 DEG C, crushing and sieving; in the fermentation of the mixed bacteria, antinutritional factors in the bean pulp are decomposed more effectively under the mutualistic synergistic effect of the clostridium butyricum and the bacillus coagulans; as a result, nutrient substances in the fermentation bean pulp are richer and more balanced; therefore, a high protein feed rich in compound probiotics is provided.

The invention discloses a harmless microorganism treatment method for cadavers of livestock and poultry. The method particularly includes the following steps: placing the cadavers in boxes, cutting, separating, crushing and slicing the cadavers, deoiling the sliced meat, fermenting and degrading the deoiled meat, performing high temperature sterilization and preparing a fertilizer, wherein during the fermenting and degrading step, thermophilus spoilage bacterium and mesophilic non-spore bacteria are employed as bacterial strains, wherein the thermophilus spoilage bacterium is composed of one or more selected from bacillus stearothermophilus, Cl.thermosaccharolyticum, clostridium butyricum, streptococcus thermophilus and bacillus macerans; and the mesophilic non-spore bacteria is composed of one or more selected from streptococcus liquefaciens, streptococcus faecalis, byssochlamys fulva, leuconostoc dextranicum, yeast and mildews. The organic fertilizer, by means of the key functions of the microorganisms, can supply various nutrient elements to crops and also can supply large amount of biotins, amino acids, vitamins, growth hormone, antibiotics and the like physiological active components that are necessary in growth of the crops.

A composite preparation for reducing a diarrhea rate of weaned piglets is disclosed. The composite preparation is prepared by mixing an oligosaccharide-acidifier solution and a probiotic fermentation liquor according to a volume ratio of 1:(3-5). Each mL of the oligosaccharide-acidifier solution contains 2500-3500 mug of oxalic acid, 30-60 mug of tartaric acid, 50-100 mug of malic acid, 4000-5500 mug of lactic acid, 2500-4000 mug of citric acid, 0.05-0.2 g of glucose, 0.05-0.1 g of isomaltooligosaccharide, 0.02-0.06 g of fructooligosaccharide and 0.08-0.15 g of chitosan oligosaccharide. The probiotic fermentation liquor is prepared by mixing a mixed fermentation liquor of lactobacillus plantarum and pediococcus pentosaceus and a clostridium butyricum fermentation liquor according to a volume ratio of (2-4):1. A preparing method of the composite preparation is also disclosed. The composite preparation reduces the diarrhea rate of weaned piglets, reduces the death rate of piglets, and boosts productive performance and immunity of piglets.

The invention relates to preparation and application of a diarrhoea-resistant type special composite microecological preparation for piggies, belongs to the field of a feed additive, solves the technical problems of the special composite microecological preparation prepared from various beneficial microorganisms and having favorable biology diarrhoea-resistant effects, for the piggies, the product strain compatibility and the feeding effect stability, and relates to the diarrhoea-resistant type special composite microecological preparation for piggies. The diarrhoea-resistant type special composite microecological preparation is characterized by being formed by combining bacterium powder I, with bacterium powder II and a carrier, wherein the bacterium powder I is coated composite lactic acid bacteria consisting of coated enterococcus faecalis and pediococcus acidilactici; the bacterium powder II is composite bacillus formed by mixed fermentation of three probiotics of bacillus subtilis, bacillus coagulans and clostridium butyricum. Various beneficial microorganisms in the diarrhoea-resistant type special composite microecological preparation for piggies have the characteristics of being high in bacterium restraining capacity, rapid in propagation, capable of realizing synergistic symbiosis, rich in produced enzymes and high in enzyme activity. A technique of united fermentation of various bacterium strains and strain compatibility is adopted for preparing the diarrhoea-resistant type special composite microecological preparation, so that the diarrhoea-resistant type special composite microecological preparation is worth of wide popularization and application.

The invention discloses a method for breeding black soldier fly larvae. The method includes steps of S1, uniformly mixing livestock and poultry feces which is fermented at the high temperatures, wheatbran, humus, brown sugar and clostridium butyricum with one another according to a mass ratio to prepare black soldier fly breeding bait; S2, breeding the larvae, to be more specific, inoculating 1-2g of newly hatched black soldier fly larvae in every 1 kg of black soldier fly breeding bait, carrying out culture on the newly hatched black soldier fly larvae at the temperatures of 25-35 DEG C for6-8 days and carrying out screening to obtain the mature black soldier fly larvae. The mass ratio of the livestock and poultry feces to the wheat bran to the humus to the brown sugar to the clostridium butyricum is 900-1000:40-60:20-40:4-5:1-3. The method has the advantages that growth and ingestion of the black soldier fly larvae can be promoted by the aid of the method, the livestock and poultry feces consumption speeds can be increased, the culture cycle of the black soldier fly larvae can be shortened, the protein content of black soldier fly can be increased, the black soldier fly larvaeobtained by the aid of the method can be used as a raw material for active feed or protein-source feed, and the method has a great application prospect.

The invention discloses a processing method of a viable clostridium butyricum preparation and a culture medium composition. The processing method comprises the following steps: carrying out activation and enlarge cultivation on strains; culturing the strains by utilizing a seeding tank; carrying out fermentation cultivation by utilizing a fermentation tank, wherein a fermentation culture medium comprises the following components: corn flour, peptone, beef extract powder, yeast extracts, dipotassium phosphate, monopotassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ferrous sulfate, sodium acetate and cysteine salt. According to the processing method, by optimizing the culture medium and a processing process, the fermentation cultivation is short, and the maturing rate of spores is high; furthermore, a thallus collection method is further optimized, so that the production process is greatly simplified, the energy consumption can be substantially reduced, and the yield of active thalli can be improved.

The invention discloses a microbial preparation, an aquatic feed and an aquaculture method and relates to the technical field of aquaculture. The microbial preparation comprises bdellovibrio and probiotic. Optimally, the probiotic is selected from any one of lactobacillus bulgaricus, lactobacillus delbrueckii, enterococcus faecalis, lactobacillus casei, lactobacillus acidophilus, rhodopseudomonaspalustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum. The microbial preparation has thecharacteristics of capability of increasing food intake of aquaculture animals, capability of promoting growth rate, survival rate and immunity of organisms, and the like. Besides, the microbial preparation is capable of effectively preventing and treating the problems of diseases caused by pathogenic bacteria, such as vibrio parahaemolyticus, vibrio harveyi, luminous vibrio, vibrio alginolyticus,vibrio campbellii, vibrio fluvialis, vibrio vulnificus, aeromonas, vibrio cholerae, pseudomonas and salmonella.