Processing method of viable clostridium butyricum preparation and culture medium composition

A technology of Clostridium butyricum and live bacteria preparations, applied in the biological field, can solve the problems of long fermentation time and low spore rate of fermentation broth, etc., achieve short fermentation culture time, high spore maturity rate, and optimize bacterial collection The effect of the method

Active Publication Date: 2014-04-09
GUANGDONG HINAPHARM PHARMA CO LTD
View PDF4 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves how clostridia are grown by reducing their growth cycle length while increasing cell size during culturing. It achieves this through optimization with specific media compositions or processes that shorten up the overall duration of each step. Additionally, it allows for more efficient collection of bacterial colonies from these solutions compared to traditional methods like incubating them at room temperature beforehand. Overall, this new approach makes producing high-quality crops faster and easier than current techniques.

Problems solved by technology

The technical problem addressed by this patented method for producing clostridiocolve (C.) Bacteria that are resistant against various factors such as pH value, temperature, oxygen concentration, nutrients, enzyme activity, antibodies, growth conditions, and others during production processes remains unexplored despite their promise being explanted from use in medical applications like treating gastrointestinal disorders caused by harmful substances released into bloodstream fluid due to ingestion thereof.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] 1. Activation and expansion of culture strains:

[0077] 1) Activated strain: The strain of Clostridium butyricum is separated and purified by streaking on RCM solid medium. The RCM solid medium (enhanced clostridium multiplication medium) is produced by Qingdao Haibo Biotechnology Co., Ltd., and its ingredients are: tryptone 10g / L, beef powder 10g / L, yeast powder 3g / L, and glucose 5g / L, soluble starch 1g / L, sodium chloride 5g / L, sodium acetate 3g / L, L-cysteine ​​hydrochloride 0.5g / L, agar powder 20g / L, PH6.8-7.0.

[0078] 2) Expand the culture of strains: select a single colony on the RCM solid medium, inoculate it in 200 ml of RCM liquid medium, and place it in an anaerobic bag for anaerobic culture at 37°C for 48 hours. The RCM liquid medium is the same as above, but no agar is added.

[0079] 2. Cultivating strains in seed tanks:

[0080] A 50-liter first-level seed tank is connected to 200 ml of RCM liquid culture medium, and nitrogen is introduced into the tank from th...

Embodiment 2

[0089] 1. Activation and expansion of culture strains:

[0090] 1) Activated strain: The strain of Clostridium butyricum is separated and purified by streaking on RCM solid medium. The RCM solid medium (enhanced clostridium multiplication medium) is produced by Qingdao Haibo Biotechnology Co., Ltd., and its ingredients are: tryptone 10g / L, beef powder 10g / L, yeast powder 3g / L, and glucose 5g / L, soluble starch 1g / L, sodium chloride 5g / L, sodium acetate 3g / L, L-cysteine ​​hydrochloride 0.5g / L, agar powder 20g / L, PH6.8-7.0.

[0091] 2) Expand the culture of strains: select a single colony on the RCM solid medium, inoculate it in 200 ml of RCM liquid medium, and place it in an anaerobic bag for anaerobic culture at 37°C for 48 hours. The RCM liquid medium is the same as above, but no agar is added.

[0092] 2. Cultivating strains in seed tanks:

[0093] A 50-liter first-level seed tank is connected to 200 ml of RCM liquid culture medium, and nitrogen is introduced into the tank from th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a processing method of a viable clostridium butyricum preparation and a culture medium composition. The processing method comprises the following steps: carrying out activation and enlarge cultivation on strains; culturing the strains by utilizing a seeding tank; carrying out fermentation cultivation by utilizing a fermentation tank, wherein a fermentation culture medium comprises the following components: corn flour, peptone, beef extract powder, yeast extracts, dipotassium phosphate, monopotassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ferrous sulfate, sodium acetate and cysteine salt. According to the processing method, by optimizing the culture medium and a processing process, the fermentation cultivation is short, and the maturing rate of spores is high; furthermore, a thallus collection method is further optimized, so that the production process is greatly simplified, the energy consumption can be substantially reduced, and the yield of active thalli can be improved.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner GUANGDONG HINAPHARM PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap