Preparation and application of Clostridium butyricum and live Clostridium butyricum preparation

A technology for Clostridium butyricum and viable bacteria preparations, applied in the field of microbial fermentation, can solve the problems of not involving the influence of production performance, unsuitable for industrial production, complex medium components, etc., to improve the balance of intestinal flora, improve Eggshell quality, low cost effect

Active Publication Date: 2017-03-08
湖北绿雪生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Patents CN201110116927, CN201110126498, etc. relate to the production and processing methods of Clostridium butyricum, but these methods generally have complex medium components, high fermentation costs, and are not suitable for industrial production.
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  • Preparation and application of Clostridium butyricum and live Clostridium butyricum preparation
  • Preparation and application of Clostridium butyricum and live Clostridium butyricum preparation
  • Preparation and application of Clostridium butyricum and live Clostridium butyricum preparation

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Embodiment 1

[0037] Embodiment-clostridium butyricum ( Clostridium butyricum ) Isolation and Identification of LXKJ-1

[0038] Clostridium butyricum ( Clostridium butyricum ) LXKJ-1 was isolated from the intestinal tract of healthy pigs. Firstly, the collected samples were diluted with normal saline and heated in a water bath at 80 °C for 10 min to kill non-spore bacteria; Cultivate in tanks for 24-30 hours, conduct preliminary identification of strains based on colony morphology and cell morphology, and select strains with cultural characteristics, colony morphology, and cell morphology that conform to the characteristics of Clostridium butyricum, and continuously streak and purify them for three generations, and mark them separately. Proceed to the next step of identification and screening.

[0039] Further identification of Clostridium butyricum ( Clostridium butyricum ) The taxonomic status of LXKJ-1, the present invention uses traditional classification methods and molecular classi...

Embodiment 2

[0078] Embodiment Clostridium dibutyricum tertiary fermentation (200L fermentation tank)

[0079] (1) Preparation of the primary seed liquid: the prepared Clostridium butyricum ( Clostridium butyricum ) LXKJ-1 original bacterial liquid was inoculated on the primary seed medium for seed preparation. The primary seed was prepared in a blue cap bottle with a preparation volume of 1 L. The medium was as follows: glucose 15 g / L, L-cysteine ​​1 g / L, sodium thioglycolate 1 g / L, K 2 HPO 4 2 g / L, yeast extract powder 5 g / L, soybean peptone 20 g / L, NaCl 5 g / L, sodium alginate 5 g / L, pH 6.5-7.0, culture temperature 36 ℃, culture time 24 hours.

[0080] (2) Preparation of the secondary seed solution: the prepared primary Clostridium butyricum ( Clostridium butyricum)LXKJ-1 seed solution was inoculated in a 20L fermenter according to the inoculum amount of 5%, as the secondary seed solution, and the formula of the secondary seed solution was the secondary seed medium. The specific medi...

Embodiment 3

[0083] The preparation of embodiment clostridium tributyricum freeze-dried bacterial powder and live bacteria preparation

[0084] (1) Collect the thalli by centrifugation: the Clostridium butyricum obtained above ( Clostridium butyricum ) The fermentation broth of LXKJ-1 was centrifuged with a tube centrifuge at a centrifugal speed of 12000rpm and a feed rate of 200L / h.

[0085] (2) Freeze-drying of the bacteria: add the sterilized 20% skim milk powder suspension and 10% trehalose to the bacteria sludge obtained by the above centrifugation according to the weight ratio of 1:3, mix well, carry out vacuum freeze-drying, and freeze-dry Time 24h.

[0086] (3) Freeze-dried bacteria powder: freeze-dried and pulverized bacteria to obtain freeze-dried bacteria powder. It has been determined that the number of live bacteria in the bacteria powder produced by the above method can reach up to 1.0*10 11 More than cfu / g.

[0087] (4) Preparation of Clostridium butyricum live bacteria ...

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Abstract

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a production method and application of Clostridium butyricum and a live Clostridium butyricum preparation. Background technique [0002] At present, with the further expansion of people's breeding scale, in order to improve the breeding benefits, the extensive use of various antibiotics as growth promoters has become a common phenomenon, and its disadvantages have become increasingly prominent, such as drug residues, reports of drug-resistant pathogens, animal intestinal The imbalance of intestinal flora and environmental pollution have seriously threatened human health and food safety. As a safe, efficient, non-polluting and non-resistant antibiotic substitute product, microecological preparations are gradually used in the breeding industry as disease prevention and growth promotion preparations. [0003] Microecological preparations are biological preparations and live bact...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/75A23K10/18C12R1/145
CPCC12N1/20C12N1/205C12R2001/145
Inventor 王丽张仁文姜文明黄跃忠冯丽萍
Owner 湖北绿雪生物科技有限公司
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