50 results about "Bacillus perfringens" patented technology

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The invention belongs to the technical field of gene engineering, and particularly relates to recombinant lactobacillus for expressing clostridium perfringens multiple antigens of targeted dendritic cells as well as a construction method and application of the recombinant lactobacillus. The recombinant lactobacillus contains an expression vector for expressing a fusion gene; the fusion gene comprises sequences for coding and expressing a PgsA '-fimA fusion protein, a LysM-Fba fusion protein, an LP1261-NetB fusion protein, a phi54 targeting element and a Pas escape element; a sequence for coding the PgsA '-fimA fusion protein is shown as SEQ ID NO: 1, a sequence for coding the LysM-Fba fusion protein is shown as SEQ ID NO: 2, and a sequence for coding the LP1261-NetB fusion protein is shown as SEQ ID NO: 3. When the recombinant lactobacillus is used for immunizing animals, mucosal immunity, cellular immunity and humoral immunity reactions can be induced, the immunocompetence of organisms can be comprehensively enhanced, and the recombinant lactobacillus can be used for preparing oral vaccines for prevention and control of chicken necrotic enteritis.

The invention relates to bacillus velezensis Bv-GD2 and application of the bacillus velezensis Bv-GD2. The classification name of the bacillus velezensis Bv-GD2 is bacillus velezensis, and the preservation number of the bacillus velezensis Bv-GD2 is GDMCC No: 66722. The strain is wide in antibacterial spectrum and high in tolerance, has the enzyme production capacity and the antibacterial active substance production capacity, and can remarkably inhibit common intestinal pathogens such as clostridium perfringens, salmonella, staphylococcus aureus and escherichia coli, relieve intestinal lesions, improve the health degree of the intestinal environment and improve the growth performance of livestock and poultry.

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

The utility model relates to a clostridium perfringens rapid detection reagent box, and relates to the field of detection reagent boxes. The device comprises a main box shell, a middle partition plate is installed in the main box shell, a first partition plate and a second partition plate are installed in the middles of the two sides of the middle partition plate respectively, and the first partition plate and the second partition plate are both fixedly connected with the middle partition plate in a sealed mode; the space of the main box shell is divided into a reaction area, a cooling area, a material area and a sample storage area by the middle partition plate, the first partition plate and the second partition plate, and a box cover capable of being turned over to be opened is further mounted on the upper end face of the main box shell. Through reasonable partition design, the functions of reaction, cooling, material storage, sample preservation and the like are integrated in one reagent box, so that the detection process is more ordered, and the detection efficiency is improved. The ultraviolet lamp and the heating lamp are arranged, so that the disinfection, sterilization and temperature control functions can be realized, various requirements in the detection process are met, and the accuracy and the reliability of a detection result are improved.

The application discloses antagonistic Bacillus of Clostridium perfringens type G and application, the Bacillus is Bacillus sonorensis CB-6, and is classified and named as Bacillus sonorensis CB-6, the preservation number is CCTCC NO: M20251373, the preservation date is June 12, 2025, and the preservation unit is China Center for Type Culture Collection, which is located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.The sterile fermentation supernatant of Bacillus sonorensis CB-6 provided by the application has neutral pH value, can inhibit Clostridium perfringens with high activity, the bacteriostatic effect is not dependent on organic acid such as lactic acid, the bacteriostatic effect is significant and stable, can significantly enhance the immunity of laying hens after Clostridium perfringens infection and improve the egg production rate, and solves the problem that laying hens are prone to necrotic enteritis during the egg production period and are not suitable for using antibiotics for prevention and control.

The application belongs to the field of biological products, and relates to a single-domain antibody of epsilon toxin of Clostridium perfringens and application thereof. The application specifically discloses a single-domain antibody of epsilon toxin of Clostridium perfringens, and the amino acid sequence of the single-domain antibody is shown in SEQ ID NO. 16. The single-domain antibody can neutralize epsilon toxin in vivo, can treat animals poisoned by epsilon toxin, and has application prospect.

The application belongs to the technical field of biotechnology, and particularly relates to a Clostridium perfringens bacteriophage lytic enzyme produced by Bacillus subtilis, a preparation method and application thereof, an optimized Clostridium perfringens bacteriophage lytic enzyme coding nucleic acid sequence according to the codon bias of Bacillus subtilis, and an optimized Clostridium perfringens bacteriophage lytic enzyme coding nucleic acid sequence as shown in SEQ ID NO. 1. The preparation method of the Clostridium perfringens bacteriophage lytic enzyme is as follows: firstly, a recombinant expression vector containing a Clostridium perfringens bacteriophage lytic enzyme gene is constructed, and then the vector is transferred into a Bacillus subtilis competent cell to obtain a Bacillus subtilis strain recombinant engineering bacterium. After the recombinant strain is induced to express, a fermentation liquor with Clostridium perfringens bacteriophage lytic enzyme activity can be obtained. The fermentation liquor can specifically inhibit the growth of Clostridium perfringens, and provides a safe, efficient and orally-taken preparation for treating animal diseases caused by Clostridium perfringens.

The invention belongs to the technical field of biology, and particularly relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine as well as a preparation method and application thereof. The invention relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine, the bivalent inactivated vaccine comprises two inactivated strain antigens, and the antigens are a bactrian camel A type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35194 and a double hump D type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC No.35195. According to the application, a newly found bactrian camel clostridium perfringens strain is used as an antigen. The 1059 ST type clostridium perfringens are separated from intestinal tracts of young bactrian camels for the first time, and the 1059 type (A type) and 675 type (D type) clostridium perfringens are inactivated to prepare the bivalent inactivated vaccine.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus paralicheniformis CA10001 as well as application and a product thereof. The bacillus paralicheniformis CA10001 has been preserved in the China General Microbiological Culture Collection Center on February 22, 2024, and the preservation number of the bacillus paralicheniformis CA10001 is CGMCC No. 29876. The strain has relatively strong antibacterial ability, heat resistance, acid resistance and cholate resistance to clostridium perfringens, and can be used for improving feed quality and improving animal growth performance.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant lactobacillus plantarum for preventing and controlling chicken necrotic enteritis as well as a preparation method and application of the recombinant lactobacillus plantarum. The invention provides an oral vaccine vector capable of co-expressing various key virulence factor antigens (FimB, NetB, CnaA and FBA) of clostridium perfringens, and by fusing dendritic cell (DC) targeting molecules (nano antibody Phi74), the efficiency of recognizing and taking in the vaccine vector by full-time antigen presenting cells is remarkably improved, so that the immunogenicity is enhanced. When the recombinant lactobacillus is used for immunizing animals, mucosal immunity, cellular immunity and humoral immunity reactions can be induced, the immunity of organisms is comprehensively enhanced, and a safe and efficient oral immunization scheme suitable for large-scale breeding is established for treating chicken necrotic enteritis.

The invention relates to the technical field of microorganisms, in particular to a method for reducing the dosage of antibiotics for treating clostridium perfringens infection, a bactericidal composition, bacteriophage and application. According to the method, the clostridium perfringens bacteriophage RDP-CP-2310008 and one or more antibiotics are used in a combined mode, and the clostridium perfringens bacteriophage RDP-CP-2310008 and the one or more antibiotics are used in a combined mode. The clostridium perfringens bacteriophage RDP-CP-2310008 is preserved in the China General Microbiological Culture Collection Center on November 29, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45778. The clostridium perfringens bacteriophage RDP-CP-2310008 can be used The bactericidal composition is prepared from the following effective components: the clostridium perfringens bacteriophage RDP-CP-2310008, and one or more antibiotics used for resisting the clostridium perfringens. The invention further discloses application of the clostridium perfringens phage RDP-CP-2310008 in preparation of a medicine for preventing and / or treating clostridium perfringens infection. The invention provides and verifies that the specific bacteriophage is combined with the antibiotics for the first time, so that the dosage of the antibiotics can be greatly reduced and can be up to 64 times, and a brand-new path is provided for solving the problem of abuse of the antibiotics in the breeding industry.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

According to the present invention, provided is a Clostridium perfringens sporulation inhibitor that contains at least one inorganic compound at such a concentration as to cause the precipitation of C. perfringens in vivo, wherein the inorganic compound is a salt of at least one selected from the group consisting of K, Na, Mg, Ca and Fe and the inorganic compound is coated with a protective layer containing a hardened vegetable oil.

The invention discloses a method for quantitatively analyzing the content of alpha2-3 acetylneuraminic acid in glycoprotein, which comprises the following steps: firstly, treating a sample containing glycoprotein by using streptococcus pneumoniae acetylneuraminidase, and continuously treating the sample containing glycoprotein by using clostridium perfringens acetylneuraminidase in the same solution; centrifugally separating and taking a separating solution containing two acetylneuraminic acid products outside the centrifugal tube; and detecting and collecting two hydrolysates 2, 7-dehydrated acetylneuraminic acid and the signal intensity of acetylneuraminic acid in a negative ion mode, and calculating the percentage content of alpha2-3 linked acetylneuraminic acid by using a formula. According to the method, the sensitivity of quantitative analysis of alpha2-3 acetylneuraminic acid can be improved, and the time consumption of detection and analysis is reduced.

This invention relates to the field of Clostridium perfringens antigen technology, and particularly to a Clostridium perfringens α-toxin-ferritin nanoparticle antigen, its preparation method, and its application. The Clostridium perfringens α-toxin-ferritin nanoparticle antigen is assembled from αm2ST protein and FeSC protein. The αm2ST protein is expressed using an α-protein mutant expression plasmid, and the FeSC protein is expressed using a ferritin expression plasmid. The nucleotide sequence of the α-protein mutant expression plasmid is shown in SEQ ID NO: 1, and the nucleotide sequence of the ferritin expression plasmid is shown in SEQ ID NO: 2. The Clostridium perfringens α-toxin-ferritin nanoparticle antigen provided by this invention can effectively stimulate humoral and cellular immunity, providing a new approach for the development of Clostridium perfringens subunit vaccines.

This invention belongs to the field of microbial technology, and relates to a *Pediococcus pentosacchari* strain derived from geese, its isolation and screening method, and its application. The *Pediococcus pentosacchari* strain is *Pediococcus pentosacchari*. Pediococcus pentosaceus L2, deposited at the Guangdong Provincial Microbial Culture Collection Center, accession number GDMCC No:67207. This invention, *Pediococcus pentosaceus* L2, was isolated and screened from the cecum of healthy Magang geese. Its source is reliable and readily available, possessing safety and probiotic properties. It exhibits good antibacterial activity against *Clostridium perfringens*, demonstrating excellent probiotic potential for application in feed additives, probiotic preparations, and medications for treating geese infected with *Clostridium perfringens*.

The invention discloses a nucleic acid aptamer specifically bound with clostridium perfringens. A nucleotide sequence of the nucleic acid aptamer comprises a nucleotide sequence as shown in SEQ ID NO: 1. According to the nucleic acid aptamer specifically bound with clostridium perfringens, the dissociation constant Kd of binding with the clostridium perfringens is 66.91 + / -22.02 nM, and the aptamer only has high affinity to the clostridium perfringens and has no affinity to other bacteria, so that the aptamer has high specificity in binding with the clostridium perfringens, and therefore, the nucleic acid aptamer specifically bound with the clostridium perfringens can be applied to the field of biosynthesis of the clostridium perfringens. The aptamer provided by the invention has strong affinity and high specificity to the clostridium perfringens, and can realize efficient and rapid detection of the clostridium perfringens by taking the aptamer as a recognition element, thereby providing a new method for on-site rapid monitoring of supervision departments.

The invention relates to the technical field of animal feed additives, in particular to a composition with bacteriostatic activity and application thereof. A screening antibacterial experiment of the plant essential oil and the organic acid shows that the composition of the plant essential oil and the organic acid, which is reasonably proportioned, has remarkable antibacterial activity on escherichia coli, salmonella typhimurium, shigella flexneri and clostridium perfringens, and particularly has a very good synergistic interaction effect. The composition of the plant essential oil and the organic acid is preferably thymol, carvacrol and sorbic acid, and the optimal compounding ratio is 3: 1: 1. The plant essential oil and organic acid composition provided by the invention has the advantages of being good in antibacterial effect, low in production cost, natural, non-toxic and the like, provides an effective treatment means for weaned piglets with bacterial diarrhea, and has huge potential commercial value.

The invention is applicable to the technical field of microorganisms, and provides a lactobacillus paracasei Jlu-CH9 and application thereof, the lactobacillus paracasei Jlu-CH9 is preserved in China General Microbiological Culture Collection Center on January 4, 2026, the preservation number is CGMCC No.37281, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain Jlu-CH9 obtained by separation has certain tolerance to acid and cholate, has no hemolysis phenomenon, and is sensitive to various antibiotics; the compound has good inhibitory activity on dipeptidyl peptidase IV and has a certain potential of reducing blood sugar. The compound has relatively good inhibitory activity on a plurality of pathogenic bacteria including salmonella typhimurium, staphylococcus aureus, escherichia coli, clostridium perfringens and pseudomonas aeruginosa; the traditional Chinese medicine composition has good prevention and treatment effects on a DSS-induced mouse colitis model, and can repair intestinal barriers, regulate immune balance, remodel intestinal flora homeostasis and relieve colonic atrophy.

The invention discloses a dual-fluorescence PCR detection kit for simultaneously detecting clostridium septicum and clostridium ganodontotrichum and a primer probe group of the dual-fluorescence PCR detection kit, and belongs to veterinary detection of animal epidemic diseases in the technical field of biological detection. The invention discloses a dual-fluorescence PCR (polymerase chain reaction) detection kit for simultaneously detecting clostridium septicum and clostridium emphasis. The dual-fluorescence PCR detection kit comprises a primer probe group for detecting alpha genes of the clostridium septicum and a primer probe group for detecting the clostridium emphasis. The kit is simple and convenient in detection operation, strong in specificity, high in sensitivity and good in repeatability, and not only can be used for qualitatively detecting clostridium septicum and clostridium gangrene, but also can be used for realizing accurate quantification; technical support is provided for detection of clostridium septicum and clostridium gangrene, epidemic situation epidemiological investigation, epidemic disease monitoring and purification, strain screening and quality monitoring and stable production and supply conservation in the vaccine production process, and the method plays an important role.

The invention relates to clostridium perfringens in excrement as a molecular marker for grading and judging fatty liver diseases related to metabolic dysfunction and application of the clostridium perfringens, the clostridium perfringens contains alpha toxin CPA according to the types of main toxins generated by the clostridium perfringens, the coding gene of the alpha toxin CPA is plc gene, and the content of the alpha toxin CPA is determined by quantitatively detecting the plc gene. The molecular level detection of the clostridium perfringens in the excrement sample is realized; besides, the clostridium perfringens further contain nirA genes related to nitrogen metabolism, the nirA genes participate in the metabolic process related to microbial ammonia production, the functional state of the intestinal ammonia-producing microorganisms is reflected by detecting the expression level of the nirA genes, stable acquisition of the molecular characteristics of the intestinal microorganisms related to the MASLD is achieved, and the application prospect is broad. A molecular basis is provided for analyzing the correlation between the abnormal ammonia metabolism and the occurrence and development of the MASLD.

The invention relates to the technical field of microorganisms, particularly provides a novel clostridium perfringens phage and further provides application of the novel clostridium perfringens phage. The preservation number of the novel clostridium perfringens phage provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.46656, and the novel clostridium perfringens phage is classified and named as the clostridium perfringens phage. According to the invention, a brand-new virulent clostridium perfringens phage vBCppII Hohhot is separated and identified for the first time, the homology of a genome sequence of the strain and a known phage is low, and a clostridium perfringens phage resource library is expanded; meanwhile, the bacteriophage has efficient splitting capacity and can rapidly inhibit and remove host bacteria.

The invention discloses a reusable dual-mode aptamer biosensor for detecting clostridium perfringens, which is characterized by comprising a competitive chain CP-C, a CeO2 (at) PANI nano composite material, a closed bipolar electrode system and an aptamer functionalized magnetic bead probe. According to the invention, the high-affinity nucleic acid aptamer is combined with the specific extracellular electron transfer mechanism of the clostridium perfringens viable bacteria for the first time, a new viable bacteria detection sensing strategy free of nucleic acid extraction and target gene amplification is constructed, and the biosensor can specifically recognize the viable clostridium perfringens, and can be used for detecting the viable bacteria of the clostridium perfringens. According to the present invention, with the detection method, the false positive result caused by the existence of dead bacteria is avoided, the detection can be rapidly completed within 3 min, the voltage signal and the G value signal are mutually proved, the false positive or false negative of the single signal detection is effectively avoided, the detection limit is as low as 2.50 CFU / mL, and the excellent anti-interference ability in the complex food matrix such as chicken and milk is provided; the core element ITO glass electrode can be continuously and repeatedly used for 12 times, and the performance is not obviously attenuated.

The application belongs to the technical field of microorganisms, and particularly relates to a parali-bacillus licheniformis CA10001, application and product thereof. The parali-bacillus licheniformis CA10001 has been preserved in the China General Microbiological Culture Collection Center on February 22, 2024, and the preservation number is CGMCC No.29876. The strain has strong bacteriostatic capacity on clostridium perfringens, is heat-resistant, acid-resistant and bile salt-resistant, and can be used for improving feed quality and improving animal growth performance.

The application relates to a culture medium for improving the biomass and fermentation product yield of Clostridium autoethanogenum and a preparation method and application thereof, and belongs to the technical field of Clostridium autoethanogenum fermentation. Each liter of the culture medium comprises the following components: ammonium chloride, arginine, lysine, methionine, potassium dihydrogen phosphate, L-cysteine hydrochloride, trace element mother liquor, vitamin mother liquor and phosphoric acid. The culture medium is optimized, yeast extract powder (YE) is not relied on, a plurality of amino acids are added as nitrogen sources, the external contamination risk can be reduced in the later production, and the metabolism of the bacteria is promoted; the proportion of sulfur sources is adjusted by optimizing the proportion of cysteine and sodium sulfide, a suitable growth environment is created for Clostridium autoethanogenum, the strain grows rapidly, the contamination risk in the fermentation process is reduced, and the product content is improved; finally, a surfactant is added, the clumping phenomenon in the Clostridium autoethanogenum fermentation process is improved, the gas-liquid mass transfer efficiency is improved, the growth and metabolism of the strain are promoted, and the application value is high in industrialized fermentation.

The present application belongs to the technical field of genetic engineering, and particularly relates to a recombinant plant lactobacillus for preventing and controlling chicken necrotic enteritis as well as a preparation method and application thereof. The present application provides an oral vaccine carrier capable of co-expressing multiple key virulence factor antigens (FimB, NetB, CnaA, FBA) of Clostridium perfringens, and the efficiency of the vaccine carrier being recognized and taken by professional antigen presenting cells is significantly improved through fusion of dendritic cell (DC) targeting molecules (nanobody Phi74), so as to enhance immunogenicity. Immunization of animals using the recombinant lactobacillus can induce mucosal immunity, cellular immunity and humoral immunity response, and comprehensively enhance the immune ability of the body, thereby establishing a safe, efficient and suitable oral immunization scheme for large-scale breeding for treating chicken necrotic enteritis.

Provided are compositions, feeds, rations, drinking water and methods of use thereof for inducing protective immunity against or preventing Clostridium perfringens infection and / or necrotic enteritis in poultry, optionally as single dose vaccines. The composition comprises live virulent Clostridium perfringens (CP) and a mucosal adjuvant, such as cholera toxin (CT), which is for administered at or after hatch by for example oral delivery. The composition can be used with an immunomodulatory composition administered in ovo before hatching or a boost composition. The composition can be administered as a single dose.

The invention provides a specific targeting antibacterial peptide aiming at clostridium perfringens as well as a preparation method and application thereof, and belongs to the technical field of bioengineering, and the sequence of the specific targeting antibacterial peptide aiming at clostridium perfringens is as shown in SEQ ID No.1. A peptide fragment WYSPQWWNHHRH with clostridium perfringens affinity obtained through screening of a phage display peptide library is used as a targeting peptide fragment, a broad-spectrum antibacterial peptide GKEFKRIVKWPWWPWRR is used as a functional region, the peptide fragment WYSPQWWNHHRH and the broad-spectrum antibacterial peptide GKEFKRIVKWPWWRR are connected through a flexible structure GGG to construct the antibacterial peptide with a clostridium perfringens targeting effect, the antibacterial peptide shows an excellent targeting effect, and under the condition that the influence on other bacteria is small, the antibacterial peptide with the clostridium perfringens targeting effect is achieved. And the compound has efficient antibacterial activity on clostridium perfringens. In addition, the specific targeting antibacterial peptide W1LI aiming at the clostridium perfringens has relatively low hemolytic activity and cytotoxicity.