120 results about "Bacillus perfringens" patented technology

The invention provides application of an Alzheimer's disease intestinal flora biomarker combination in preparation of a product for detecting AD (Alzheimer's disease). The marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, clostridium erysipelas, helicobacter, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas, and is characterized in that the marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas. And the composition is any one or a combination of at least two of bacillus pumilus and odorobacter. According to the present invention, the abundance level of the intestinal flora biomarker combination is adopted as the detection index, and the kit is used for the early auxiliary diagnosis of the Alzheimer's disease, has characteristics of high detection precision, convenience, rapidness, safety and non-invasive property, and provides important clinical guiding significance for the auxiliary diagnosis of AD-related indexes.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the field of microorganisms, in particular to bacillus subtilis, a fungicide and application of the fungicide. The invention discloses a bacillus subtilis strain, which is classified and named as bacillus subtilis, has a strain number of GXGD-YZ-2483, is preserved in Guangdong Microbial Culture Collection Center on March 13, 2025, and has a preservation number of GDMCC No: 66014. The bacillus subtilis can tolerate the high temperature of 70-90 DEG C, has excellent self-heat production performance, and can produce lignin degrading enzymes (laccase, manganese peroxidase and / or lignin peroxidase) and inhibit the growth of pathogenic bacteria (escherichia coli, salmonella, clostridium perfringens and / or aspergillus flavus). When the bacillus subtilis or the microbial inoculum prepared from the bacillus subtilis is used for biologically composting organic wastes, a lignin barrier can be efficiently cracked by secreting a lignin degrading enzyme system, lignin degradation, nitrogen retention and pathogenic bacteria inactivation can be synchronously realized by virtue of high temperature resistance, self-heat production characteristics and enzyme activity stability, the composting period is shortened, and the yield of the organic wastes is improved. The humification quality is obviously improved.

Bacillus strains useful for preventing and / or controlling Clostridium perfringens infections and necrotic enteritis in monogastric animals, such as swine and poultry, and for improving the overall health, feed conversion rates and body weight gain of such animals, as well as compositions comprising the Bacillus strains and various methods of using the Bacillus strains in animal feed and animal feed additives.

The invention discloses a culture medium for A type clostridium perfringens and C type clostridium perfringens, a fermentation culture method and application of fermentation toxin production. Wherein the culture medium comprises the following components: tryptone, # imgabs0 # peptone, casein peptone, a yeast extract, lactoalbumin hydrolysate, trihydroxymethyl aminomethane, sodium acetate and glucose. According to the present invention, through the specific formula composition, the A-type clostridium perfringens and the C-type clostridium perfringens can adapt to fermentation in the fermentation tank, and in the fermentation culture process, the toxin production ability of the A-type clostridium perfringens and the C-type clostridium perfringens can be significantly improved by controlling the stirring speed, the pH value of the fermentation liquid and the precise material supplementing manner. And the supernate obtained by fermentation is used as a raw material for preparing the vaccine, so that animals can generate high-level neutralizing antibodies aiming at the clostridium perfringens A and C type toxins after being immunized with the vaccine, the death of the animals caused by infection of the clostridium perfringens A and C type toxins is avoided, and the method has practical popularization and application values.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention discloses bacillus velezensis, a microbial preparation as well as a preparation method and application of the microbial preparation, and relates to the technical field of microorganisms. The bacillus velezensis strain is preserved in the China Center for Type Culture Collection, and is named as bacillus velezensis XNW-BLSYB1, the preservation number is CCTCC NO: M 2025721, the preservation time is April 8, 2025, and the 16S rDNA sequence of the bacillus velezensis strain is as shown in SEQID No.1. The bacillus velezensis strain has the advantages that the bacillus velezensis strain is named as bacillus velezensis XNW-BLSYB1; the spore formed by the bacillus velezensis XNW-BLSYB1 strain can survive at high temperature, and can inhibit the reproductive and respiratory syndrome virus and inhibit the activity of bacteria such as streptococcus suis, infectious actinobacillus pleuropneumoniae, clostridium perfringens, pasteurella multocida and escherichia coli.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The invention belongs to the technical field of microorganisms, and discloses a heat-resistant and acid-base-resistant clostridium perfringens bacteriophage XHX-CP-18P and application thereof, the bacteriophage is preserved in the China Center for Type Culture Collection (CCTCC) on March 17, 2025, and the preservation number is CCTCC NO: M 2025492. The invention also provides a bactericide product prepared from the bacteriophage. The bacteriophage has good acid and alkali resistance under the condition that the pH is 3-13, has heat resistance under the condition that the temperature is 30-60 DEG C, and ensures that the activity of the bacteriophage is not lost. The bacteriophage has a good lysis effect on clostridium perfringens, is strong in infection ability, can infect more host cells with a very small amount of viruses, can effectively lyse A-type and G-type hosts, and can replace antibiotics to resist corrosion of gastric acid, so that intestinal diseases of the hosts are effectively treated.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

The invention relates to the technical field of intestinal pathogen detection, in particular to a multiplex PCR (polymerase chain reaction) primer group, a kit and a method for detecting pathogens causing calf diarrhea. The invention provides a primer group for detecting diarrheagenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium, and a multiple PCR (Polymerase Chain Reaction) method for detecting the diarrheagenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium is established on the basis of the primer group. According to the primer group and the multiple PCR method disclosed by the invention, the mixed infection and single infection conditions of diarrheogenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium in a sample to be detected can be determined through one-time multiple PCR reaction, and compared with a common PCR method, the primer group and the multiple PCR method are more convenient and quicker, and time and resources are saved; and compared with real-time fluorescent quantitative PCR, the method is more cost-saving and convenient to operate.

The invention belongs to the technical field of gene engineering, and particularly relates to recombinant lactobacillus for expressing clostridium perfringens multiple antigens of targeted dendritic cells as well as a construction method and application of the recombinant lactobacillus. The recombinant lactobacillus contains an expression vector for expressing a fusion gene; the fusion gene comprises sequences for coding and expressing a PgsA '-fimA fusion protein, a LysM-Fba fusion protein, an LP1261-NetB fusion protein, a phi54 targeting element and a Pas escape element; a sequence for coding the PgsA '-fimA fusion protein is shown as SEQ ID NO: 1, a sequence for coding the LysM-Fba fusion protein is shown as SEQ ID NO: 2, and a sequence for coding the LP1261-NetB fusion protein is shown as SEQ ID NO: 3. When the recombinant lactobacillus is used for immunizing animals, mucosal immunity, cellular immunity and humoral immunity reactions can be induced, the immunocompetence of organisms can be comprehensively enhanced, and the recombinant lactobacillus can be used for preparing oral vaccines for prevention and control of chicken necrotic enteritis.

The invention relates to a novel bacillus subtilis (B.subtilis) with a strong inhibition effect on clostridium perfringens (C. perfringens), and an application of the bacillus subtilis as a probiotic.

The invention relates to bacillus velezensis Bv-GD2 and application of the bacillus velezensis Bv-GD2. The classification name of the bacillus velezensis Bv-GD2 is bacillus velezensis, and the preservation number of the bacillus velezensis Bv-GD2 is GDMCC No: 66722. The strain is wide in antibacterial spectrum and high in tolerance, has the enzyme production capacity and the antibacterial active substance production capacity, and can remarkably inhibit common intestinal pathogens such as clostridium perfringens, salmonella, staphylococcus aureus and escherichia coli, relieve intestinal lesions, improve the health degree of the intestinal environment and improve the growth performance of livestock and poultry.

The present invention relates to a strain of Clostridium butyricum and its application, belonging to the field of microbiology. The Clostridium butyricum JBH-BD1 of the present invention is preserved in the China Center for Type Culture Collection, with the preservation number CCTCC NO: M2022151. The Clostridium butyricum of the present invention is natural, safe, acid-resistant, alkali-resistant, heat-resistant, and has strong abilities to produce protease, amylase and organic acids. At the same time, it has antibacterial effects on Escherichia coli, Salmonella, Staphylococcus aureus, Clostridium perfringens, Pasteurella multocida of porcine origin and Actinobacillus pleuropneumoniae, and can convert inorganic ferrous iron into organic ferrous iron; when used in the aquaculture industry, it can not only regulate the balance of intestinal flora, promote the growth of livestock and poultry, but also effectively improve the utilization rate of iron element, reduce its addition amount, prevent iron deficiency anemia in livestock and poultry, improve the absorption and utilization efficiency of iron by livestock and poultry, and increase the aquaculture benefits.

In one aspect, the invention relates to compounds useful as hybrid antibiotics, acting as both activators of the ClpP protease and inhibitors of RNA polymerase activity. Also disclosed are methods of using the disclosed compounds in the treatment of infectious diseases such as, for example, biofilm-mediated diseases and infectious diseases due to prosthetic joint infections, intracellular bacteria, and Gram-positive bacteria (e.g., Gram-positive bacteria selected from Bacillus anthracis, Bacillus cereus, Bacillus subtilis, Clostridium difficile, Clostridium tetani, Clostridium botulinum, Clostridium perfringens, Corynebacterium diphtheria, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Listeria ivanovii, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus hyicus, Staphylococcus intermedius, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

This invention belongs to the field of biotechnology and discloses a monoclonal antibody that specifically binds to the recombinant protein of Clostridium perfringens (Cp) β2 toxin, a hybridoma cell line, and its applications. This invention provides a monoclonal antibody that specifically binds to the β2 toxin protein and a hybridoma cell line CPB2-2A4 (accession number CCTCC NO: C2024312) that secretes this monoclonal antibody. The monoclonal antibody reacts only with the Cp β2 toxin protein and does not cross-react with other pathogens infecting cattle and sheep, exhibiting extremely high specificity. The kit utilizes competitive ELISA technology, using a mouse monoclonal antibody that specifically binds to the Cp β2 toxin recombinant protein as a competing antibody, enabling the detection of Cp β2 toxin antibodies in serum from various animal sources, avoiding species limitations of sample sources. Furthermore, the kit exhibits good reproducibility. The kit has relatively relaxed technical requirements during operation and can be widely used in production for applications such as Cp epidemiological surveys and vaccine immunization level monitoring.

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

The present invention relates to the technical field of vaccine preparation, and in particular to an ASP-ETX fusion protein and a Clostridium perfringens epsilon toxin nanoparticle vaccine and a preparation method thereof. The ASP-ETX fusion protein of the present invention comprises Clostridium perfringens epsilon toxin and an activation-related secretory protein; the amino acid sequence of the ASP-ETX fusion protein is shown in SEQ ID NO.1. The Clostridium perfringens epsilon toxin nanoparticle vaccine is obtained by co-incubating the ASP-ETX fusion protein with cell membrane-wrapped nanoparticles. The vaccine exhibits excellent biosafety in both cells and animals, and induces the production of up to 10 5 ETX-specific antibodies with a neutralizing antibody titer of up to 64 can protect mice against 100×LD 50 GST-ETX toxin attack has great potential to replace traditional ETX vaccines.

The invention discloses bacillus velezensis LYBV022 and application thereof in preparation of a preparation for inhibiting clostridium perfringens infection, and belongs to the technical field of microorganisms. The invention discloses a bacillus velezensis LYBV022, and the preservation number of the bacillus velezensis LYBV022 is GDMCC No: 66952. The bacillus velezensis LYBV022 disclosed by the invention has a relatively good antibacterial ability on clostridium perfringens, staphylococcus aureus, escherichia coli and the like, so that the bacillus velezensis LYBV022 has a clinical application potential.

The utility model relates to a clostridium perfringens rapid detection reagent box, and relates to the field of detection reagent boxes. The device comprises a main box shell, a middle partition plate is installed in the main box shell, a first partition plate and a second partition plate are installed in the middles of the two sides of the middle partition plate respectively, and the first partition plate and the second partition plate are both fixedly connected with the middle partition plate in a sealed mode; the space of the main box shell is divided into a reaction area, a cooling area, a material area and a sample storage area by the middle partition plate, the first partition plate and the second partition plate, and a box cover capable of being turned over to be opened is further mounted on the upper end face of the main box shell. Through reasonable partition design, the functions of reaction, cooling, material storage, sample preservation and the like are integrated in one reagent box, so that the detection process is more ordered, and the detection efficiency is improved. The ultraviolet lamp and the heating lamp are arranged, so that the disinfection, sterilization and temperature control functions can be realized, various requirements in the detection process are met, and the accuracy and the reliability of a detection result are improved.

The application discloses a broad-spectrum bacteriostatic Clostridium subsp. and a preparation method and application thereof, and belongs to the technical field of microorganisms. The strain is named as DMS028, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 38049. The strain has broad-spectrum bacteriostatic activity, can simultaneously inhibit gram-negative bacteria Aeromonas hydrophila in aquaculture, gram-positive bacteria Clostridium perfringens in livestock and poultry breeding, and plant diseases such as Exserohilum longipes and Fusarium oxysporum, and overcomes the defects of narrow bacteriostatic spectrum of existing microbial preparations. The preparation method adopts two-stage temperature anaerobic culture, and adds lysine methyl ester, leucine ethyl ester, palmitoyl glycine and lauroyl arginine ethyl ester as an antibacterial substance synergist and a stabilizer, and has the advantages of simple process, convenient operation, no need of complex equipment, easy scale production, efficient high-activity bacterial body and antibacterial product.

The application discloses antagonistic Bacillus of Clostridium perfringens type G and application, the Bacillus is Bacillus sonorensis CB-6, and is classified and named as Bacillus sonorensis CB-6, the preservation number is CCTCC NO: M20251373, the preservation date is June 12, 2025, and the preservation unit is China Center for Type Culture Collection, which is located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.The sterile fermentation supernatant of Bacillus sonorensis CB-6 provided by the application has neutral pH value, can inhibit Clostridium perfringens with high activity, the bacteriostatic effect is not dependent on organic acid such as lactic acid, the bacteriostatic effect is significant and stable, can significantly enhance the immunity of laying hens after Clostridium perfringens infection and improve the egg production rate, and solves the problem that laying hens are prone to necrotic enteritis during the egg production period and are not suitable for using antibiotics for prevention and control.

The application belongs to the field of biological products, and relates to a single-domain antibody of epsilon toxin of Clostridium perfringens and application thereof. The application specifically discloses a single-domain antibody of epsilon toxin of Clostridium perfringens, and the amino acid sequence of the single-domain antibody is shown in SEQ ID NO. 16. The single-domain antibody can neutralize epsilon toxin in vivo, can treat animals poisoned by epsilon toxin, and has application prospect.

The present invention belongs to the field of microbial applications, and specifically relates to a bacteriocin Thurisin A2 and its application. The bacteriocin Thurisin A2 has an amino acid sequence as shown in SEQ ID NO.1, and can be synthesized by a polypeptide solid-phase synthesis method or a liquid-phase polypeptide synthesis method, or the coding gene of the bacteriocin Thurisin A2 can be expressed in an engineered bacterium. It has a simple structure, is safe and low-toxic, is easy to synthesize, can effectively kill Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and Listeria monocytogenes, and has no hemolytic activity against eukaryotic animal red blood cells. It has biosafety and can be used to prepare food preservatives, feed additives, antibacterial drugs, etc., and has broad application prospects.

The application relates to the technical field of biological medicine, and discloses application of maslinic acid or a derivative thereof in preparation of a product for treating Clostridium perfringens infection. The application verifies that the maslinic acid has a good inhibiting effect on Clostridium perfringens through in-vitro experiments and in-vivo infection models, and has a good treatment effect on intestinal infection of Clostridium perfringens in mammals and avian necrotic enteritis, thereby providing a new approach and thought for treatment of Clostridium perfringens infection, and having important clinical significance and application value.

The application belongs to the technical field of biotechnology, and particularly relates to a Clostridium perfringens bacteriophage lytic enzyme produced by Bacillus subtilis, a preparation method and application thereof, an optimized Clostridium perfringens bacteriophage lytic enzyme coding nucleic acid sequence according to the codon bias of Bacillus subtilis, and an optimized Clostridium perfringens bacteriophage lytic enzyme coding nucleic acid sequence as shown in SEQ ID NO. 1. The preparation method of the Clostridium perfringens bacteriophage lytic enzyme is as follows: firstly, a recombinant expression vector containing a Clostridium perfringens bacteriophage lytic enzyme gene is constructed, and then the vector is transferred into a Bacillus subtilis competent cell to obtain a Bacillus subtilis strain recombinant engineering bacterium. After the recombinant strain is induced to express, a fermentation liquor with Clostridium perfringens bacteriophage lytic enzyme activity can be obtained. The fermentation liquor can specifically inhibit the growth of Clostridium perfringens, and provides a safe, efficient and orally-taken preparation for treating animal diseases caused by Clostridium perfringens.

The invention belongs to the technical field of biology, and particularly relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine as well as a preparation method and application thereof. The invention relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine, the bivalent inactivated vaccine comprises two inactivated strain antigens, and the antigens are a bactrian camel A type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35194 and a double hump D type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC No.35195. According to the application, a newly found bactrian camel clostridium perfringens strain is used as an antigen. The 1059 ST type clostridium perfringens are separated from intestinal tracts of young bactrian camels for the first time, and the 1059 type (A type) and 675 type (D type) clostridium perfringens are inactivated to prepare the bivalent inactivated vaccine.

The invention belongs to the technical field of microorganisms, and discloses a clostridium perfringens bacteriophage CP-XHX1 and application thereof, the bacteriophage is preserved in the China Center for Type Culture Collection (CCTCC) on June 17, 2024, and the preservation number is CCTCC NO: M 20241270. The invention also provides a bactericide product prepared from the bacteriophage. The cracking rate of the clostridium perfringens A, E and G reaches 100%, the toxic and side effects are small, and the safety is high. The bacteriophage has a good lysis effect on clostridium perfringens, is strong in infection ability, can infect more host cells with a very small amount of viruses, and can replace antibiotics, so that the intestinal diseases of hosts are effectively treated.

The invention provides a subunit vaccine and application thereof in resisting avian clostridium perfringens, and the subunit vaccine is characterized in that non-toxic CPA and NetB proteins are truncated, and three gene sequences of auxiliary protein metallopeptidase are connected in series and fused through Linker to obtain a recombinant gene. The recombinant protein with good solubility and high expression quantity can be obtained from the recombinant gene under the induction conditions that the IPTG concentration is 0.2 mM and the temperature is 25 DEG C. Immunogenicity detection on the N-C-M recombinant protein finds that the N-C-M recombinant protein can induce SPF chicken to generate antibodies. Immune protection test results show that a subunit vaccine prepared from the N-C-M protein and an adjuvant finds that 100% immune protection effect can be achieved for immunizing 100 [mu] g / chicken and 200 [mu] g / chicken, and the subunit vaccine can be used as a subunit vaccine for resisting various toxin types.