Rapid detection reagent box for clostridium perfringens

Abstract

The utility model relates to a clostridium perfringens rapid detection reagent box, and relates to the field of detection reagent boxes. The device comprises a main box shell, a middle partition plate is installed in the main box shell, a first partition plate and a second partition plate are installed in the middles of the two sides of the middle partition plate respectively, and the first partition plate and the second partition plate are both fixedly connected with the middle partition plate in a sealed mode; the space of the main box shell is divided into a reaction area, a cooling area, a material area and a sample storage area by the middle partition plate, the first partition plate and the second partition plate, and a box cover capable of being turned over to be opened is further mounted on the upper end face of the main box shell. Through reasonable partition design, the functions of reaction, cooling, material storage, sample preservation and the like are integrated in one reagent box, so that the detection process is more ordered, and the detection efficiency is improved. The ultraviolet lamp and the heating lamp are arranged, so that the disinfection, sterilization and temperature control functions can be realized, various requirements in the detection process are met, and the accuracy and the reliability of a detection result are improved.

Description

Technical Field

[0001] This application relates to the technical field of testing kits, and in particular to a rapid testing kit for Clostridium perfringens. Background Technology

[0002] Clostridium perfringens is an anaerobic pathogen that causes necrotic enteritis in farmed broilers. Necrotic enteritis leads to decreased appetite, lethargy, diarrhea, and even death in broilers. Clostridium perfringens can also spread through feces, causing widespread infection and resulting in severe economic losses for poultry farms. Therefore, rapid diagnosis of Clostridium perfringens infection is crucial for poultry farms.

[0003] Currently, the main methods for detecting Clostridium perfringens include conventional culture methods, ELISA kits, and multiplex PCR technology. These methods are complex to operate and costly. Considering the explosive phenomenon of Clostridium perfringens fermenting milk and the correlation between this phenomenon and bacterial count, a rapid detection kit for Clostridium perfringens was designed to facilitate rapid detection at the farm. Utility Model Content

[0004] To achieve rapid detection at the breeding site, this application provides a rapid detection kit for Clostridium perfringens.

[0005] The rapid detection kit for Clostridium perfringens provided in this application adopts the following technical solution:

[0006] A rapid detection kit for Clostridium perfringens includes a main shell with a central partition. A first partition and a second partition are respectively installed on the central sides of the central partition. Both the first and second partitions are sealed and fixedly connected to the central partition. The central partition, the first partition, and the second partition divide the space of the main shell into a reaction zone, a cooling zone, a material zone, and a sample storage zone. A flip-open lid is also installed on the upper surface of the main shell. An ultraviolet lamp corresponding to the reaction zone, cooling zone, material zone, and sample storage zone is fixedly installed on the inner side of the lid. A heating lamp corresponding to the reaction zone and cooling zone is also fixedly installed on the inner side of the lid.

[0007] By adopting the above technical solution, the main chamber space is rationally divided into a reaction zone, a cooling zone, a material zone, and a sample storage zone using a central partition, a first partition, and a second partition. This ensures that each step in the Clostridium perfringens detection process can proceed in an orderly manner, avoiding mutual interference and improving detection efficiency. The reaction zone houses the water bath. The cooling zone contains a rectangular plastic or stainless steel basin, used for rapid cooling of test tubes containing culture medium after water bath sterilization. The material zone holds the reagents and equipment required for rapid detection, including sampling tubes, reaction tubes, dilution tubes, a balance, weighing paper, pipettes, pipette tips, test tube racks, and diluent. The sample storage zone holds the collected samples. An ultraviolet lamp installed on the lid disinfects all areas, effectively preventing bacterial contamination and ensuring the accuracy of the test results. Furthermore, the lid is locked to the main chamber using a snap lock, and heating lamps control the temperature of the reaction and cooling zones to meet different temperature requirements during the detection process, further improving the reliability of the detection.

[0008] Optionally, the upper surface of the middle partition is flush with the upper surface of the main housing, and a positioning groove is provided in the middle of the upper surface of the middle partition. The height of the first partition is lower than the upper surface of the main housing, and the upper surface of the second partition is higher than the upper surface of the main housing.

[0009] By adopting the above technical solution, the upper surface of the middle partition is flush with the upper surface of the main housing and has a positioning groove, which facilitates the sealing connection when the lid is closed. The height of the first partition is lower than the upper surface of the main housing, which facilitates the simultaneous use of ultraviolet lamps and heating lamps. The upper surface of the second partition is higher than the upper surface of the main housing, which facilitates the installation and sealing of the lid, and can also play a certain role in positioning and separation, ensuring the independence and sealing of each area and reducing the influence of external factors on the testing process.

[0010] Optionally, a water bath is placed in the cooling zone, and an insertion hole for the water bath is provided on the side of the cooling zone. Two sets of auxiliary supports are symmetrically installed on both sides of the water bath, and the water bath is fixedly connected to the auxiliary supports.

[0011] By adopting the above technical solution, a water bath is placed in the cooling zone, allowing for more uniform and stable cooling of the test samples. The side-mounted socket facilitates power connection and operation of the water bath, improving the ease of use of the equipment. The auxiliary support enhances the stability of the water bath and makes it easier to place and remove the equipment within the reagent box.

[0012] Optionally, the auxiliary support includes a suspension frame and a grip frame. The suspension frame is fixedly installed on one side of the water bath, and the grip frame is installed at the head of the suspension frame and is rotatably connected to the suspension frame.

[0013] By adopting the above technical solution, the auxiliary support consists of a suspension frame and a grip frame, and the grip frame is rotatably connected to the suspension frame. This design allows the operator to flexibly adjust the angle of the grip frame as needed, making it convenient to move and operate the water bath, thus improving the practicality and convenience of the equipment.

[0014] Optionally, the suspension frame includes a base bracket and a vertical rod bracket. The base bracket is fixedly installed on the lower end face of the water bath, and the vertical rod bracket is vertically installed at both ends of the base bracket and fixedly connected to the base bracket. The head of the vertical rod bracket is also provided with a rotating shaft for the gripping frame to be rotated and installed. The rotating shaft is integrally formed with the vertical rod bracket.

[0015] By adopting the above technical solution, the base bracket and vertical support structure of the suspension frame can be firmly fixed to the water bath, providing stable support for the gripping frame. The rotating shaft and vertical support are integrally formed, ensuring the flexibility and stability of the gripping frame's rotation, allowing operators to easily lift and move the water bath.

[0016] Optionally, the grip frame includes a grip bar, a slant bracket, and a connecting sleeve rotatably mounted on a rotating shaft. The slant bracket is fixedly mounted at both ends of the grip bar, and the connecting sleeve is disposed at the upper end of the slant bracket, and the connecting sleeve is integrally formed with the slant bracket.

[0017] By adopting the above technical solution, the gripping rod, inclined frame, and connecting sleeve of the holding frame have a simple structure and are easy to operate. The gripping rod is easy for the operator to hold, and the design of the inclined frame and connecting sleeve allows the holding frame to be tightly connected to the suspension frame and to rotate flexibly. It can be flipped up when needed and flipped down to be stored in the suspension frame when not in use, further improving the convenience of transporting the water bath.

[0018] Optionally, the lid includes a main cover plate and a handle, the handle being installed at the center of the upper surface of the main cover plate, and both ends of the handle being rotatably connected to the main cover plate.

[0019] By adopting the above technical solution, the design of the main cover plate and handle of the reagent box facilitates opening and carrying. The handle is installed at the center of the upper surface of the main cover plate and is rotatably connected to the main cover plate at both ends, making it easier for operators to use and improving the portability of the equipment.

[0020] Optionally, a sealing strip one that mates with the positioning groove is provided on the inner side of the main cover plate, and a mating groove corresponding to the second partition is also provided on the inner side of the main cover plate. A sealing strip two is installed in the mating groove, and both the sealing strip one and the sealing strip two are fixedly connected to the main cover plate.

[0021] By adopting the above technical solution, the sealing strip one and sealing strip two set on the inner side of the main cover plate cooperate with the positioning groove and the second partition plate respectively, which can effectively enhance the sealing of the reagent box, prevent external dust, moisture and bacteria from entering the box, protect the test reagents and samples from contamination, and ensure the accuracy and reliability of the test results.

[0022] In summary, this application includes at least one of the following beneficial technical effects: Through a rational partitioned design, this application integrates reaction, cooling, material storage, and sample preservation functions into a single reagent box, making the detection process more organized and improving detection efficiency. Equipped with UV lamps and heating lamps, it achieves sterilization and temperature control functions, meeting various needs during the detection process and improving the accuracy and reliability of detection results. The auxiliary support for the water bath and the handle design of the lid make the equipment easier to move and operate, reducing the labor intensity of operators. The sealing strip effectively enhances the airtightness of the reagent box, protecting the test reagents and samples from external factors and extending their shelf life. This reagent kit has a reaction zone, cooling zone, material zone, and sample zone, allowing for immediate detection after sampling and obtaining results in a short time, ensuring rapid detection. Heating lamps and UV lamps are also included to prevent cross-contamination. Attached Figure Description

[0023] Figure 1 This is a perspective view of the overall structure in the embodiments of this application.

[0024] Figure 2 yes Figure 1 Side view of the device shown.

[0025] Figure 3 yes Figure 1 Top view of the device shown.

[0026] Figure 4 This is a perspective view of the water bath and auxiliary support in an embodiment of this application.

[0027] Figure 5 This is a perspective view of the suspension frame in the embodiments of this application.

[0028] Figure 6 This is a perspective view of the grip frame in the embodiments of this application.

[0029] Explanation of reference numerals in the attached drawings: 1. Main casing; 11. Middle partition; 111. Positioning groove; 12. First partition; 13. Second partition; 2. Reaction zone; 3. Cooling zone; 31. Water bath; 32. Insertion hole; 4. Material zone; 5. Sample storage zone; 6. Lid; 61. Main cover plate; 611. Sealing strip one; 612. Fitting groove; 613. Sealing strip two; 62. Handle; 7. Ultraviolet lamp; 8. Heating lamp; 9. Auxiliary support; 91. Suspension frame; 911. Base bracket; 912. Vertical rod frame; 913. Rotating shaft; 92. Grip frame; 921. Grip bar; 922. Slanted frame; 923. Connecting sleeve. Detailed Implementation

[0030] The present application will be further described in detail below with reference to the accompanying drawings.

[0031] This application discloses a rapid detection kit for Clostridium perfringens. Example 1

[0032] Reference Figure 1 , Figure 2 and Figure 3As shown, a rapid test kit for Clostridium perfringens includes a main shell 1. A central partition 11 is installed within the main shell 1. A first partition 12 and a second partition 13 are respectively installed on the central sides of the central partition 11. Both the first partition 12 and the second partition 13 are sealed and fixedly connected to the central partition 11. The central partition 11, the first partition 12, and the second partition 13 divide the space of the main shell 1 into a reaction zone 2, a cooling zone 3, a material zone 4, and a sample storage zone 5. A flip-open lid 6 is also installed on the upper surface of the main shell 1. An ultraviolet lamp 7, corresponding to the reaction zone 2, cooling zone 3, material zone 4, and sample storage zone 5, is fixedly installed on the inner side of the lid 6. A heating lamp 8, corresponding to the reaction zone 2 and cooling zone 3, is also fixedly installed on the inner side of the lid 6. A switch panel 13 is also provided at the front end of the main shell 1, including control switches for the ultraviolet lamp 7 and the heating lamp 8. The main casing 1 is rationally divided into a reaction zone 2, a cooling zone 3, a material zone 4, and a sample storage zone 5 by a central partition 11, a first partition 12, and a second partition 13. This ensures that each step in the Clostridium perfringens detection process can proceed in an orderly manner, avoiding mutual interference and improving detection efficiency. The reaction zone 2 houses the water bath 31. The cooling zone 3 contains a rectangular plastic or stainless steel basin, used for rapid cooling of test tubes containing culture medium after water bath sterilization. The material zone 4 houses the reagents and equipment required for rapid detection, including sampling tubes, reaction tubes, dilution tubes, a balance, weighing paper, pipettes, pipette tips, test tube racks, and diluent. The sample storage zone 5 holds the collected samples. An ultraviolet lamp 7 installed on the lid 6 disinfects each area, effectively preventing bacterial contamination and ensuring the accuracy of the test results. Furthermore, the lid 6 is locked to the main shell 1 via a snap lock, and the heating lamp 8 can control the temperature of the reaction zone 2 and the cooling zone 3 to meet different temperature requirements during the testing process, further improving the reliability of the testing. The upper surface of the middle partition 11 is flush with the upper surface of the main shell 1, and a positioning groove 111 is provided in the middle of the upper surface of the middle partition 11. The height of the first partition 12 is lower than the upper surface of the main shell 1, and the upper surface of the second partition 13 is higher than the upper surface of the main shell 1. The upper surface of the middle partition 11 is flush with the upper surface of the main shell 1 and has a positioning groove 111, which facilitates the sealing connection when the lid 6 is closed. The height of the first partition 12 is lower than the upper surface of the main shell 1, which facilitates the simultaneous use of the ultraviolet lamp 7 and the heating lamp 8. The upper surface of the second partition 13 is higher than the upper surface of the main shell 1, which facilitates the installation and sealing of the lid 6, and also plays a certain role in positioning and separation, ensuring the independence and sealing of each area, and reducing the influence of external factors on the testing process.

[0033] Reference Figure 3As shown, a water bath 31 is placed in the cooling zone 3, and an insertion hole 32 for the water bath 31 is provided on the side of the cooling zone 3. Two sets of auxiliary supports 9 are symmetrically installed on both sides of the water bath 31, and the water bath 31 is fixedly connected to the auxiliary supports 9. The placement of the water bath 31 in the cooling zone 3 allows for cooling of the test samples through a water bath, making the cooling process more uniform and stable. The insertion hole 32 on the side facilitates the power connection and operation of the water bath 31, improving the ease of use of the equipment. The auxiliary supports 9 enhance the stability of the water bath 31 and make it easier to place and remove the equipment from the reagent box.

[0034] Reference Figure 2 As shown, the lid 6 includes a main cover plate 61 and a handle 62. The handle 62 is installed at the center of the upper surface of the main cover plate 61, and both ends of the handle 62 are rotatably connected to the main cover plate 61. The design of the main cover plate 61 and handle 62 of the lid 6 facilitates the opening and carrying of the reagent box. The handle 62 is installed at the center of the upper surface of the main cover plate 61 and rotatably connected to the main cover plate 61 at both ends, making it easier for operators to use and improving the portability of the equipment. A sealing strip 611 that mates with the positioning groove 111 is provided on the inner side of the main cover plate 61, and a mating groove 612 corresponding to the second partition 13 is also provided on the inner side of the main cover plate 61. A second sealing strip 613 is installed in the mating groove 612. Both the first sealing strip 611 and the second sealing strip 613 are fixedly connected to the main cover plate 61. The sealing strip 611 and sealing strip 613 on the inner side of the main cover plate 61 cooperate with the positioning groove 111 and the second partition 13 respectively, which can effectively enhance the sealing of the reagent box, prevent external dust, moisture and bacteria from entering the box, protect the test reagents and samples from contamination, and ensure the accuracy and reliability of the test results.

[0035] Example 2

[0036] Reference Figure 4 and Figure 5As shown, the auxiliary support 9 includes a suspension frame 91 and a gripping frame 92. The suspension frame 91 is fixedly installed on one side of the water bath 31, and the gripping frame 92 is installed at the head of the suspension frame 91 and is rotatably connected to the suspension frame 91. The auxiliary support 9, composed of the suspension frame 91 and the gripping frame 92, allows the operator to flexibly adjust the angle of the gripping frame 92 as needed, facilitating the handling and operation of the water bath 31 and improving the practicality and convenience of the equipment. The suspension frame 91 includes a base bracket 911 and a vertical rod bracket 912. The base bracket 911 is fixedly installed on the lower end face of the water bath 31, and the vertical rod bracket 912 is vertically installed at both ends of the base bracket 911 and is fixedly connected to the base bracket 911. The head of the vertical rod bracket 912 is also provided with a rotating shaft 913 for the gripping frame 92 to rotate and install. The rotating shaft 913 is integrally formed with the vertical rod bracket 912. The design of the base bracket 911 and vertical rod bracket 912 of the suspension bracket 91 allows it to be firmly fixed to the water bath 31, providing stable support for the gripping frame 92. The rotating shaft 913 is integrally formed with the vertical rod bracket 912, ensuring the flexibility and stability of the gripping frame 92's rotation, allowing operators to easily lift and move the water bath 31.

[0037] Reference Figure 4 and Figure 6 As shown, the grip frame 92 includes a grip rod 921, a slant bracket 922, and a connecting sleeve 923 rotatably mounted on a rotating shaft 913. The slant bracket 922 is fixedly mounted at both ends of the grip rod 921, and the connecting sleeve 923 is located at the upper end of the slant bracket 922, and the connecting sleeve 923 is integrally formed with the slant bracket 922. The grip rod 921, slant bracket 922, and connecting sleeve 923 of the grip frame 92 have a simple structure and are easy to operate. The grip rod 921 is easy for the operator to hold, and the design of the slant bracket 922 and connecting sleeve 923 allows the grip frame 92 to be tightly connected to the suspension frame 91 and to rotate flexibly. It can be flipped up when needed and flipped down to be stored in the suspension frame 91 when not in use, further improving the convenience of transporting the water bath 31.

[0038] The implementation principle of a rapid detection kit for Clostridium perfringens according to an embodiment of this application is as follows: In use, open the kit, remove the items for sampling and detection from material area 4, collect samples using sampling tubes, place the collected samples in the storage area, weigh 1g of sample into a dilution tube using a balance, mix well, and simultaneously remove the water bath 31 from reaction area 2. Connect the water bath 31 to a power source, add an appropriate amount of water to the water bath 31, and set the temperature of the water bath 31 to 100℃. After reaching the temperature, place the reaction tube in the water bath 31 and maintain it for 2 minutes. Remove it and place it in cooling area 3 (with an appropriate amount of water added) to rapidly cool the reaction tube. Add an appropriate amount of water to the water bath 31 and set the temperature of the water bath 31 to 46℃. After the reaction tube cools, use a pipette to draw 1mL of sample solution and place it at the bottom of the reaction tube. Place the reaction tube in the water bath 31 and maintain it for 5 hours, observing and recording the changes in the reaction tube during this period. After the reaction is complete, drain the water from the water bath 31 and the cooling zone 3, return the water bath 31 to the reaction zone 2, turn on the heating lamp 8 to dry the water vapor in the detection chamber, and after the water vapor is removed, turn on the ultraviolet lamp 7 and keep it for 30 minutes to disinfect and sterilize the detection chamber for the next use.

[0039] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A rapid detection kit for Clostridium perfringens, comprising a main casing (1), characterized in that: The main housing (1) is equipped with a central partition (11). A first partition (12) and a second partition (13) are respectively installed on the central parts of both sides of the central partition (11). The first partition (12) and the second partition (13) are sealed and fixedly connected to the central partition (11). The central partition (11), the first partition (12) and the second partition (13) divide the space of the main housing (1) into a reaction zone (2), a cooling zone (3), a material zone (4) and a sample storage zone (5). The upper end of the main housing (1) is also equipped with a flip-open lid (6). An ultraviolet lamp (7) that cooperates with the reaction zone (2), the cooling zone (3), the material zone (4) and the sample storage zone (5) is fixedly installed on the inner side of the lid (6). A heating lamp (8) corresponding to the reaction zone (2) and the cooling zone (3) is also fixedly installed on the inner side of the lid (6).

2. The rapid detection kit for Clostridium perfringens according to claim 1, characterized in that: The upper surface of the middle partition (11) is flush with the upper surface of the main shell (1), and a positioning groove (111) is provided in the middle of the upper surface of the middle partition (11). The height of the first partition (12) is lower than the upper surface of the main shell (1), and the upper surface of the second partition (13) is higher than the upper surface of the main shell (1).

3. The rapid detection kit for Clostridium perfringens according to claim 2, characterized in that: The cooling zone (3) is equipped with a water bath (31), and the side of the cooling zone (3) is also provided with a socket (32) that matches the water bath (31). Two sets of auxiliary supports (9) are symmetrically installed on both sides of the water bath (31), and the water bath (31) is fixedly connected to the auxiliary supports (9).

4. The rapid detection kit for Clostridium perfringens according to claim 3, characterized in that: The auxiliary support (9) includes a suspension frame (91) and a grip frame (92). The suspension frame (91) is fixedly installed on one side of the water bath (31), and the grip frame (92) is installed on the head of the suspension frame (91) and is rotatably connected to the suspension frame (91).

5. The rapid detection kit for Clostridium perfringens according to claim 4, characterized in that: The suspension frame (91) includes a base bracket (911) and a vertical rod bracket (912). The base bracket (911) is fixedly installed on the lower end face of the water bath (31). The vertical rod bracket (912) is vertically installed at both ends of the base bracket (911) and is fixedly connected to the base bracket (911). The head of the vertical rod bracket (912) is also provided with a rotating shaft (913) for the gripping frame (92) to be rotatably installed. The rotating shaft (913) and the vertical rod bracket (912) are integrally formed.

6. The rapid detection kit for Clostridium perfringens according to claim 5, characterized in that: The grip frame (92) includes a grip bar (921), a slant frame (922), and a connecting sleeve (923) rotatably mounted on a rotating shaft (913). The slant frame (922) is fixedly mounted on both ends of the grip bar (921), and the connecting sleeve (923) is located at the upper end of the slant frame (922). The connecting sleeve (923) and the slant frame (922) are integrally formed.

7. The rapid detection kit for Clostridium perfringens according to claim 6, characterized in that: The box cover (6) includes a main cover plate (61) and a handle (62). The handle (62) is installed at the center of the upper surface of the main cover plate (61), and both ends of the handle (62) are rotatably connected to the main cover plate (61).

8. The rapid detection kit for Clostridium perfringens according to claim 7, characterized in that: The inner side of the main cover plate (61) is provided with a sealing strip (611) that cooperates with the positioning groove (111), and the inner side of the main cover plate (61) is also provided with a mating groove (612) corresponding to the second partition (13). A sealing strip (613) is installed in the mating groove (612). Both the sealing strip (611) and the sealing strip (613) are fixedly connected to the main cover plate (61).

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