Bacteriostatic and deodorant compound preparation for pets and preparation method thereof

Abstract

The invention provides an antibacterial and deodorant compound preparation for pets and application thereof. The antibacterial and deodorant compound preparation comprises 30-40% of an enzyme preparation, 20-30% of a plant extract, 20-30% of probiotic powder and 0-10% of inorganic salt and a filling aid. Ammonia can be efficiently removed, and the yield of ammonia gas in excrement is effectively reduced; bacteria are efficiently inhibited, and escherichia coli, salmonella, MRSA, clostridium perfringens and the like are effectively inhibited; feces odor is reduced, and feces odor substances such as indole, cadaverine, spermidine, putrescine and skatole in pet feces are effectively reduced; safety and harmlessness are realized; possible negative effects of chemically synthesized substances on pet health are avoided; and the influence on the clotting of the cat litter is reduced to the greatest extent, so that the use effect of the cat litter is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of pet product application, and in particular to a pet-use antibacterial and deodorizing composite preparation and a preparation method thereof. Background Art

[0002] As pets play an increasingly important role in human life, their health issues are attracting more and more attention. Pet feces, as an intuitive indicator of health, directly reflects the digestive status. Excessive ammonia production means that the protein is not completely digested, which damages the intestinal epithelial cells, metabolism, and flora composition, acid-base balance, and reduces the activity of digestive enzymes, thereby affecting nutrient absorption, while causing pet health and a large amount of ammonia emissions. Sulfides (such as hydrogen sulfide), ammonium (ammonia), and aliphatic compounds (such as butyric acid and skatole) produced by feces and urine not only pollute the environment, but also have a negative impact on the physiological functions of humans and animals, stimulate the olfactory nerves, affect the respiratory center, and damage respiratory function. They are highly toxic. As the scale of pet breeding continues to expand, fecal pollution and odor problems are becoming increasingly serious. According to statistics, the number of pets in China has exceeded 100 million, and the amount of feces produced each year is huge.

[0003] The current pet feces deodorant market mostly relies on single functional substances such as yucca extract or inorganic salt systems such as sodium bicarbonate, lacks consideration from the digestion perspective, and has poor deodorization effects. At the same time, problems such as cat litter clumping limit its popularization and application, and the deodorant has a single highlight. Households have a stronger demand for pet feces deodorization. Currently, the types of products on the market are limited, mainly chemical deodorants, and biological and physical deodorants are in the early stages of development. With the increasing demand for pet feces treatment, the current deodorant technology needs to be innovated, especially for pet feces treatment. The types need to be rich and environmentally friendly, and can reduce ammonia emissions and pet feces odor. Summary of the invention

[0004] In order to effectively add deodorizing substances to feces odorous substances, the present invention provides a compound antibacterial and deodorizing preparation for pets and a preparation method thereof, by synergistically compounding substances such as biological enzymes, probiotics, plant extracts and inorganic salts, to solve the technical problem that the current deodorizing effect of cat litter is poor, reduce the abundance of odorous substances and pathogenic bacteria in pet feces, reduce the risk of pet urinary tract infection, and minimize the effect of cat litter agglomeration on the quality of the deodorizing composition.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions: The first aspect of the present invention provides a composite preparation capable of deodorizing and inhibiting bacteria, comprising 30%-40% enzyme preparation, 20%-30% plant extract, 20%-30% probiotic powder, and 0-10% inorganic salt and filling auxiliary agent.

[0006] Preferably, the enzyme preparation is obtained by mixing laccase, lipase, glucose oxidase, peroxidase, pectinase and protease in a mass ratio of 5:5:1:1:3:5.

[0007] Preferably, the protease in the enzyme preparation is obtained by mixing acidic protease, neutral protease and alkaline protease in a mass ratio of 5:3:2.

[0008] Preferably, the probiotic powder is obtained by mixing Bacillus subtilis, Saccharomyces cerevisiae and Enterococcus faecalis in a mass ratio of 1:3:5.

[0009] Preferably, the plant extract is obtained by mixing dandelion extract, honeysuckle extract and scutellaria baicalensis extract in a mass ratio of 1:1:1.

[0010] Preferably, the inorganic salt and the filling aid are obtained by mixing zinc ricinoleate, sodium bicarbonate and montmorillonite in a mass ratio of 1:2:1.

[0011] The second aspect of the present invention provides a method for preparing a deodorizing and antibacterial composite preparation, the preparation method comprising: (1) Weighing enzyme preparation, probiotic powder, plant extract and inorganic salt additive according to weight, mixing and adding into a micro-particle granulator; (2) Corn starch and distilled water are mixed and heated to obtain a corn starch solution, and then the corn starch is sprayed into a micro-granulator for granulation, and low-temperature drying is performed to remove moisture to obtain a composite preparation.

[0012] Preferably, the corn starch and distilled water are mixed in a solid-liquid ratio of 1: (5-10), and the low-temperature drying temperature is 0-40°C.

[0013] The third aspect of the present invention provides the application of the deodorizing and antibacterial composite preparation. The application scenarios include but are not limited to pet feces treatment, cat litter, kennel, carpet, external use, deodorizing volatile odors of substances, etc.

[0014] The beneficial effects of the present invention are: The antibacterial and deodorizing composite preparation of the present invention, which is compounded with enzyme preparation, probiotic powder, plant extract, inorganic salt and auxiliary agent, can synergistically effectively reduce the ammonia production in feces and fecal odor substances in pet excrement, such as indole, cadaverine, spermidine, putrescine and skatole. Enzyme preparation and probiotic group can specifically decompose organic matter in pet excrement, especially substances that cause odor. The degradation of carbohydrates by enzyme preparation provides probiotics with nutrients that are easier to absorb, promotes the recovery of probiotic group, and further decomposes fecal odor substances in pet excrement.

[0015] Furthermore, the antibacterial and deodorizing composite preparation of the present invention can effectively inhibit Escherichia coli, Salmonella, methicillin-resistant Staphylococcus aureus (MRSA), Clostridium perfringens, etc. The enzyme preparation combination assists the recovery and proliferation of the probiotics and effectively squeezes the breeding space of harmful bacteria.

[0016] Furthermore, the deodorant and antibacterial composite preparation of the present invention can minimize the impact on the agglomeration of cat litter by optimizing the formula and preparation process, thereby ensuring its use effect. The present invention adopts a method of wrapping the deodorant and antibacterial composite preparation with corn starch, and sprays it into pet excrement, cat litter, kennel, and carpet when it is finally used. The starch wrapping helps the early probiotics to recover and break out of the granules, thereby starting to decompose the excrement, and minimizes the agglomeration of excrement on products such as cat litter, and the deodorant and antibacterial composite preparation of the present invention will not produce adhesion to products such as cat litter, avoiding problems such as skin allergies and respiratory discomfort in pets. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a static ammonia production device.

[0018] Figure 2 This is the effect of Example 1 on feces ammonia production. DETAILED DESCRIPTION

[0019] The present invention is further described below with reference to the embodiments.

[0020] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0021] Description: The laccase in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 10000U.

[0022] The lipase in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 100000U.

[0023] The glucose oxidase in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 10000U.

[0024] The peroxidase in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 10000U.

[0025] The pectinase in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 300000U.

[0026] The protease in the present invention is produced by Guangdong Yiduoli Biotechnology Co., Ltd., and the enzyme activity is 200000U.

[0027] In probiotic powder, the content of Bacillus subtilis is ≥1.0×10 9 CFU / g, yeast content ≥1.0×10 7 CFU / g, Enterococcus faecium content ≥1.0×10 8 CFU / g.

[0028] Dandelion extract, honeysuckle extract, Scutellaria baicalensis extract, etc. were purchased from Changsha Shiwei Biological Co., Ltd.

[0029] Zinc ricinoleate, sodium bicarbonate, bentonite, etc. were purchased from Shanghai Yuanye Biological Co., Ltd.

[0030] Cat litter is provided by Gaoyejia in Hangzhou. Example

[0031] Three different proteases were used: acidic protease, neutral protease and alkaline protease. The addition level of each enzyme when used alone was set at 50 units per gram (u / g), and the degradation of plant raw material proteins such as soybean meal was studied under their respective optimal pH conditions. See Table 1.

[0032] Table 1 Enzyme Addition level (u / g) Enzymatic environment Soluble protein content increase rate (%) Acid protease 50 pH 3.0 75.77 Neutral protease 50 pH 6.5 110.18 Alkaline protease 50 pH7.5 70.28 When used alone, neutral protease had the highest increase rate on soluble protein content at pH 6.5, reaching 110.18%.

[0033] Subsequently, these three proteases were combined in different ratios to form four different experimental groups, and the effects of different combinations on the increase rate of soluble protein content were evaluated under pH 6.5 conditions, as shown in Table 2.

[0034] Table 2 Grouping (proportion) Acid protease Neutral protease Alkaline protease Soluble protein content increase rate (%) Group 1 5 3 2 154.81 Group 2 4 3 3 137.25 Group 3 2 4 4 107.20 Group 4 2 5 3 104.42 When used in combination, the combination ratio of the first group (acid protease: 5, neutral protease: 3, alkaline protease: 2) showed the highest increase in soluble protein content, which was 154.81%, followed by the combination of the second group (acid protease: 4, neutral protease: 3, alkaline protease: 3), which was 137.25%.

[0035] It can be seen that neutral protease performed best in the single experiment, and when acidic protease and alkaline protease were added without changing the amount of neutral protease, the increase rate of soluble protein content in the first and second groups showed that the synergistic effect of different types of proteases can significantly improve the efficiency of protein degradation, which is of great significance for improving the nutritional value and application scope of raw materials. Example

[0036] Furthermore, based on Example 1, six enzymes were used: laccase, lipase, glucose oxidase, peroxidase, pectinase and protease (acid protease: 5, neutral protease: 3, alkaline protease: 2) to treat pet excrement. By changing the addition ratio of these enzymes, five different experimental combinations were formed to evaluate their effects on ammonia production. See the experimental device connection diagram for details. Figure 1 .

[0037] Pet excrement is fermented to produce ammonia. The change in ammonia content after adding the deodorizing and antibacterial compound preparation product is measured to evaluate the deodorizing effect of the product. The detection method is based on GBT 14668-1993 Determination of ammonia in air quality, Nessler's reagent colorimetric method.

[0038] Take fresh cat feces and place equal amounts in feces bottles (devices such as Figure 1 As shown in the figure), the enzyme system prepared by the first to fifth groups was added to each feces bottle at 3% of the cat litter content. The feces bottles were placed in a room at room temperature of 28°C. The ammonia production of each group after 3 days was measured using the Nessler reagent colorimetric method. The results are shown in Table 3.

[0039] The enzymatic hydrolysis reaction was carried out under controlled conditions, and the effect of the enzyme combination was evaluated by measuring the production of ammonia in the reaction system.

[0040] Table 3 Grouping (proportion) Laccase Lipase Glucose oxidase Peroxidase Pectinase Protease Ammonia production (μg) Group 1 5 5 1 1 3 5 32.82213 Group 2 4 4 2 2 3 5 38.4933 Group 3 3 3 3 3 3.5 4.5 47.59035 Group 4 4 3 2 2 5 4 33.03507 Group 5 5 4 1 1 4 5 34.85448 The first enzyme combination (laccase: 5, lipase: 5, glucose oxidase: 1, peroxidase: 1, pectinase: 3, protease: 5) produced the least amount of ammonia, which was 32.82213 μg.

[0041] The experimental results show that different enzyme combinations have a significant effect on the amount of ammonia produced, and some combinations can more effectively inhibit the generation of odor substances such as ammonia. According to the above Examples 1 and 2, the optimal enzyme ratio combination (the first group) was selected.

[0042] Example 3 (Full Combination Example) 10kg of deodorizing and antibacterial composite preparation is prepared, including laccase: 0.25kg, lipase: 0.25kg, glucose oxidase: 0.25kg, peroxidase: 0.75kg, pectinase: 0.75kg, Bacillus subtilis powder: about 0.11kg, yeast: about 0.33kg, Enterococcus faecalis: about 0.56kg, dandelion extract: about 0.33kg, honeysuckle extract: about 0.33kg, Scutellaria baicalensis extract: about 0.33kg, zinc ricinoleate: about 0.33kg, sodium bicarbonate: about 0.67kg, and the rest is filled with montmorillonite. After mixing, the mixture is added into a micro-particle granulator at a speed of 25r / min, and then a corn starch solution (2kg corn starch is dissolved in 10kg water and heated to 45°C) is sprayed into the granulator for granulation, and the mixture is dried at low temperature to remove moisture to obtain a composite preparation.

[0043] Example 4 (Example without enzyme preparation) 10kg of deodorizing and antibacterial composite preparation is prepared, including about 0.11kg of Bacillus subtilis powder, about 0.33kg of yeast, about 0.56kg of Enterococcus faecalis, about 0.33kg of dandelion extract, about 0.33kg of honeysuckle extract, about 0.33kg of Scutellaria baicalensis extract, about 0.33kg of zinc ricinoleate, about 0.67kg of sodium bicarbonate, and the rest is filled with montmorillonite and bran. After mixing, it is added into a micro-particle granulator at a speed of 25r / min, and then corn starch solution (2kg of corn starch is dissolved in 10kg of water and heated to 45°C) is sprayed into the granulator for granulation, and low-temperature drying is performed to remove moisture to obtain a composite preparation.

[0044] Example 5 (Example without probiotics) 10kg of deodorizing and antibacterial composite preparation is prepared, including laccase: 0.25kg, lipase: 0.25kg, glucose oxidase: 0.25kg, peroxidase: 0.75kg, pectinase: 0.75kg, dandelion extract: about 0.33kg, honeysuckle extract: about 0.33kg, Scutellaria extract: about 0.33kg, zinc ricinoleate: about 0.33kg, sodium bicarbonate: about 0.67kg, and the rest is filled with montmorillonite and bran. After mixing, the mixture is added into a micro-particle granulator at a speed of 25r / min, and then a corn starch solution (2kg corn starch is dissolved in 10kg water and heated to 45°C) is sprayed into the granulator for granulation, and the composite preparation is obtained by low-temperature drying to remove moisture.

[0045] Example 6 (Example without plant extract) 10kg of deodorizing and antibacterial composite preparation is prepared, including laccase: 0.25kg, lipase: 0.25kg, glucose oxidase: 0.25kg, peroxidase: 0.75kg, pectinase: 0.75kg, Bacillus subtilis powder: about 0.11kg, yeast: about 0.33kg, Enterococcus faecalis: about 0.56kg, zinc ricinoleate: about 0.33kg, sodium bicarbonate: about 0.67kg, and the rest is filled with montmorillonite and bran. After mixing, it is added into a micro-particle granulator at a speed of 25r / min, and then corn starch solution (2kg corn starch is dissolved in 10kg water and heated to 45°C) is sprayed into the granulator for granulation, and low-temperature drying is performed to remove moisture to obtain the composite preparation.

[0046] Example 7 (Example without inorganic salt and filling aid) 10kg of deodorizing and antibacterial composite preparation is prepared, including laccase: 0.25kg, lipase: 0.25kg, glucose oxidase: 0.25kg, peroxidase: 0.75kg, pectinase: 0.75kg, dandelion extract: about 0.33kg, honeysuckle extract: about 0.33kg, Scutellaria baicalensis extract: about 0.33kg, Bacillus subtilis powder: about 0.11kg, yeast: about 0.33kg, Enterococcus faecalis: about 0.56kg, and the rest is filled with bran. After mixing, it is added into a micro-particle granulator at a speed of 25r / min, and then corn starch solution (2kg corn starch is dissolved in 10kg water and heated to 45°C) is sprayed into the granulator for granulation, and low-temperature drying is performed to remove moisture to obtain a composite preparation. Example

[0047] 1. Deodorization test Cat feces fermentation produces ammonia. The change in ammonia content after adding the deodorizing and antibacterial compound preparation product is measured to evaluate the deodorizing effect of the product. The detection method is based on GBT 14668-1993 Determination of ammonia in air quality, Nessler's reagent colorimetric method.

[0048] Take fresh cat feces and place equal amounts in feces bottles (devices such as Figure 1 As shown in the figure), the deodorizing and antibacterial composite preparations prepared in Examples 2-5 were added to each feces bottle at 3% of the cat litter content, and a control group was used without adding the deodorizing and antibacterial composite preparations. The feces bottles were placed in a room at a room temperature of 28°C, and the ammonia production of each group on the first, second, third, fifth and seventh days was measured respectively, and the determination was carried out by Nessler's reagent colorimetric method. The results are shown in Table 4.

[0049] Table 4 Deodorization effect of each embodiment Group / ammonia μg Day 1 Day 3 Day 5 Day 7 Control group 12.42 26.36 47.59 53.66 Example 3 2.71 19.09 20.30 29.72 Example 4 10.60 31.82 38.49 50.02 Example 5 10.60 27.26 31.44 31.97 Example 6 8.78 30.00 53.66 42.74 Example 7 6.35 19.69 26.97 32.43 Comparison of the data of the control group with those of Example 3 shows that the antibacterial and deodorizing composite preparation of the present invention is significantly more effective in removing ammonia than other examples. Figure 2The ammonia production of the control group and Example 3 on days 1, 3, 5 and 7, as well as the percentage of inhibition of ammonia production by Example 3 are shown. Figure 2 It shows that on the first day, Example 3 has the highest inhibition on ammonia production, reaching 78.2%, followed by the fifth day, with an inhibition of 47.27%, indicating that the enzyme preparation in the antibacterial and deodorizing composite preparation has a significant effect in deodorizing.

[0050] On the other hand, when comparing Example 3 and Examples 4-7, it can be found that in the absence of one of the components of the antibacterial and deodorizing composite preparation of the present invention, the deodorizing effect is reduced to varying degrees, especially in Example 4 where the enzyme preparation is lacking, the ammonia production on the seventh day is close to that of the control group.

[0051] At the same time, it should be noted that, in the ammonia production data on the seventh day, the effect of Example 3 cannot be achieved without one of the components of the antibacterial and deodorizing composite preparation of the present invention.

[0052] 2. Antibacterial test Example 3-7 was added at a ratio of 3% to prepare an aqueous solution, and then Escherichia coli, Salmonella, Staphylococcus aureus, and Clostridium perfringens were used as indicator bacteria to conduct an antibacterial experiment. The antibacterial results are shown in Table 5. Table 5 Antibacterial test results of antibacterial deodorant composite preparation Diameter of inhibition zone / mm Escherichia coli salmonella MRSA Clostridium perfringens Example 3 10 11 10 12 Example 4 7 8 6 8 Example 5 5 6 7 6 Example 6 6 5 6 7 Example 7 9 10 8 10 As shown in Table 5, the antibacterial and deodorizing composite preparation provided in Example 3 can effectively inhibit Escherichia coli, Salmonella, MRSA and Clostridium perfringens, and has good antibacterial performance in general, while inorganic salts and filling additives have no obvious antibacterial effect.

[0053] 3. Reduce fecal odor The remaining feces of the control group in Example 8 and Example 3 were tested for fecal odor substances. The results are shown in Table 6.

[0054] Table 6 Effects of Macleaya San and glucose oxidase on dog odor substances Projectng / g Indole Cadaverine Spermidine Humus Skatole Control group 15193.67 59138.75 353.33 38547.45 254.77 Example 3 10173.88 47654.66 297.65 29845.62 120.85 Note: All the above indicators are in ng / g; As shown in Table 6, compared with the control group, the fecal odor substances in the feces using the antibacterial and deodorizing compound preparation were significantly reduced, indicating that the antibacterial and deodorizing effect was better.

[0055] The above test results show that the combination of several substances has obvious gain benefits, indicating that the combination has synergistic benefits rather than a simple superposition combination. The enzyme preparation, plant extract, probiotics and inorganic salts provided by the present invention can effectively improve the function of the composite product and have a clear amplification effect. In summary, the antibacterial and deodorizing composite preparation provided by the present invention can efficiently remove ammonia and effectively reduce the ammonia production in feces; it is highly effective in antibacterial, effectively inhibiting Escherichia coli, Salmonella, MRSA, Clostridium perfringens, etc.; it reduces fecal odor and effectively reduces fecal odor substances in pet feces, such as indole, cadaverine, spermidine, putrescine and skatole; it is safe and harmless: it avoids the negative impact of chemical synthetic substances on pet health; it prevents clumping and minimizes the impact on cat litter clumping, thereby ensuring its use effect.

[0056] The embodiments described above can be further combined or replaced, and the embodiments are only descriptions of preferred embodiments of the present invention, and do not limit the concept and scope of the present invention. Without departing from the design concept of the present invention, various changes and improvements made by ordinary technicians in this field to the technical solution of the present invention belong to the protection scope of the present invention. The protection scope of the present invention is given by the attached claims and any equivalents thereof.

Claims

1. A pet antibacterial and deodorizing composite preparation, characterized in that: The following components are included in mass percentage: Enzyme preparation 30%-40%, plant extract 20%-30%, probiotic powder 20%-30%, inorganic salt and filling aid 0-10%.

2. The deodorizing and antibacterial composite preparation according to claim 1, characterized in that: The enzyme preparation is obtained by mixing laccase, lipase, glucose oxidase, peroxidase, pectinase and protease in a mass ratio of 5:5:1:1:3:

5.

3. The composite formulation according to claim 2, characterized in that The protease in the enzyme preparation is obtained by mixing acidic protease, neutral protease and alkaline protease in a mass ratio of 5:3:

2.

4. The composite formulation according to claim 1, characterized in that The probiotic powder is obtained by mixing Bacillus subtilis, Saccharomyces cerevisiae and Enterococcus faecalis in a mass ratio of 1:3:

5.

5. The composite formulation according to claim 1, characterized in that The plant extract is obtained by mixing dandelion extract, honeysuckle extract and scutellaria baicalensis extract in a mass ratio of 1:1:

1.

6. The composite formulation according to claim 1, characterized in that The inorganic salt and the filling auxiliary agent are obtained by mixing zinc ricinoleate, sodium bicarbonate and montmorillonite in a mass ratio of 1:2:

1.

7. The method for preparing the composite formulation according to any one of claims 1 to 6, characterized in that: The system The preparation method is: (1) Weighing enzyme preparation, probiotic powder, plant extract, and optional inorganic salt and filling aid according to weight, mixing and adding into a micro-granulator; (2) Corn starch and distilled water are mixed and heated to obtain a corn starch solution, and then the corn starch is sprayed into a micro-particle granulator for granulation, and low-temperature drying is performed to remove moisture to obtain a composite preparation; the corn starch and distilled water are mixed at a material-liquid ratio of 1: (5-10), and the low-temperature drying temperature is 0-40°C.

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