17 results about "Skatole" patented technology

Embodiments of the present invention provide a pharmaceutical intervention in the neonatal pig that inhibits and delays development and activation of the HPG axis, growth of the boar testis and inhibits testicular production of testosterone and androstenone, which prevents the development of aggressive behavior in the maturing boars and the presence of boar taint in the meat. The invention comprises treatment with a combination of an androgen and an estrogen in the newborn male piglet using extended drug delivery methods, with a defined duration of no more than 12 weeks, for the purpose of inhibiting the production of testosterone and androstenone and the accumulation of the boar taint-inducing molecules androstenone and skatole in the fat.

The invention belongs to the technical field of environmental biology, and particularly relates to a key gene for degrading skatole by enterococcus haiseri ID05 and application of the key gene for degrading skatole by enterococcus haiseri ID05, and the test process of the key gene for degrading skatole by enterococcus haiseri ID05 is as follows: selecting an enterococcus haiseri IDO5 strain, an LB culture medium, an inorganic salt culture medium and reagent consumables and instruments required in the test process; the degradation rate of the enterococcus haiseri IDO5 on 100 mg / L of skatole in an inorganic salt culture medium reaches 67.01 + / -0.01%, and it is shown that the enterococcus haiseri IDO5 has the good degradation capacity on the skatole. Besides, the degradation rate of the IDO5 on skatole in sterilized pig manure wastewater reaches up to 96.10%, the degradation rate of the IDO5 on skatole in unsterilized pig manure wastewater is also 78.17%, and the enterococcus hirsuti IDO5 has good degradation capacity on skatole in pig manure wastewater and has good application potential in pig manure wastewater, so that when the pig manure wastewater is discharged, the enterococcus hirsuti IDO5 can be used for degrading the skatole in the pig manure wastewater. After being treated by enterococcus hirsuti IDO5, the wastewater can be discharged, so that the pollution to the environment is effectively reduced.

The present invention relates to the use of a probe, in particular chosen from rhodamine or a derivative thereof, for detecting at least one molecule responsible for boar odor, such as indole or skatole, said probe being chosen, for example, from the group consisting of rhodamine 6G, eosin Y, rhodamine B and rhodamine 101.

This intestinal-environment-improving agent contains a yeast deposited to the National Institute of Technology and Evaluation under international deposition number NITE BP-04012. The intestinal-environment-improving agent of the present invention can be used as a useful intestinal-flora-improving agent, a short-chain fatty-acid-producing bacteria increase or decrease inhibitor, a short-chain fatty-acid-producing agent, a putrefaction product production inhibitor, an agent used for increasing or decreasing a specific bacterial genus or group, a bowel-movement-improving agent, a secondary bile acid production inhibitor, a valeric acid production inhibitor, and the like. The putrefaction product is preferably one or more selected from indole, phenol, p-cresol, 4-ethylphenol, and skatole.

The present invention discloses a preparation process and application of Enterococcus hirae I DO5 freeze-dried powder, which has the following beneficial effects: (1) the preparation process of Enterococcus hirae I DO5 freeze-dried powder of the present invention is simpler and easier to operate than the existing preparation process of preparing Enterococcus hirae I DO5 into immobilized pellets in the prior art; (2) by preparing Enterococcus hirae I DO5 into the form of freeze-dried powder, the low temperature and low pressure during the freeze-drying process can better retain the physiological activity of the Enterococcus hirae I DO5 strain; the Enterococcus hirae I DO5 freeze-dried powder can be stably stored for a long time, reducing the need for frequent replenishment, and is more suitable for long-term use in breeding scenarios; (3) the Enterococcus hirae I DO5 freeze-dried powder has a good deodorizing effect, and the degradation rate of indole, skatole and ammonia in pig manure wastewater can reach more than 60%; (4) the protective agent solution can effectively improve the surface morphology of the Enterococcus hirae I DO5 freeze-dried powder, enhance the cell's anti-freezing ability, maintain the integrity of the cell membrane structure, and improve the freeze-dried survival rate of Enterococcus hirae I DO5.

The application provides a bacteriostatic and deodorizing composite preparation for pets and application thereof, which comprises 30-40% of enzyme preparation, 20-30% of plant extract, 20-30% of probiotic powder, and 0-10% of inorganic salt and filling aid. The application can efficiently remove ammonia and effectively reduce the ammonia gas yield in excrement; can efficiently inhibit E. coli, Salmonella, MRSA, C. perfringens and the like; can reduce fecal odor and effectively reduce fecal odor substances in pet excrement, such as indole, cadaverine, spermidine, putrescine and skatole and the like; is safe and harmless, avoids the negative influence of chemical synthetic substances on pet health, prevents clumping, and maximally reduces the influence on cat litter clumping, thereby guaranteeing the use effect.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

The invention relates to the technical field of daily chemical products, and discloses a strong toilet cleaner for removing bacteria and odor and a preparation method thereof, the strong toilet cleaner comprises 3.8-6.5 parts by weight of a surfactant, 2.5-6 parts by weight of a cyclodextrin-based bacteria and odor removing agent, 4.6-6.8 parts by weight of an organic acid, 0.8-1.3 parts by weight of a chelating agent, and 0.3-0.8 part by weight of a thickening agent; the cyclodextrin-based degerming deodorant contains quaternary ammonium salt cation antibacterial groups and can form electrostatic interaction with cell membrane surfaces of bacteria and fungi, so that the cell membranes are destroyed, growth and reproduction of the bacteria and fungi are inhibited, and the cyclodextrin-based degerming deodorant shows very strong degerming and sterilizing performance. And the beta-cyclodextrin has a cavity structure, has specific inclusion and adsorption effects on ammonia, hydrogen sulfide, skatole and other substances and gas molecules, and can achieve a good deodorization effect.

The invention belongs to the technical field of animal feed, and relates to a feed additive as well as a preparation method and application thereof in livestock feed. The feed additive is prepared from the following components in parts by mass: 10-20 parts of pericarpium citri reticulatae, 20-30 parts of radix angelicae sinensis, 8-15 parts of compound plant essential oil, 2.5-3.0 parts of compound microorganisms and 4-8 parts of gamma-aminobutyric acid. By adding the feed additive disclosed by the invention into a feed, the production performance can be improved, the immune function can be enhanced, the generation of skatole and ammonia nitrogen in excrement can be reduced, the breeding benefit of livestock is remarkably improved, and meanwhile, the feed additive is beneficial to improving the environment.

The invention relates to an enzyme gene YKSWp450 of a 3-methylindole efficient degrading bacterium P450 and application of the enzyme gene YKSWp450, based on a gene with a skatole degrading function in rhodococcus gordoni YKSW-6, a remarkably up-regulated gene YKSWp450 with a coding oxidation-reduction function enzyme is screened out through screening and amplification of a target gene, and it is proved through heterologous expression and a whole-cell biotransformation test that the gene YKSWp450 has the advantages that the gene YKSWp450 has the function of degrading skatole and has the function of degrading skatole in the rhodococcus gordoni YKSW-6, and the gene YKSWp450 has the function of degrading skatole in the rhodococcus gordoni YKSW-6; the gene has the ability of degrading skatole, both escherichia coli added with recombinant plasmids and purified enzyme can degrade 100 mg / L of skatole, the removal rate reaches 75% or above, and the gene can be effectively used for treating skatole pollution in the environment.

The invention relates to a pig feed additive and a preparation method and application thereof, and the pig feed additive is prepared from the following raw materials in parts by weight: 20-25 parts of a basic carrier, 24-32 parts of an acremonium terricola culture, 18-26 parts of polyrhachis vicina roger extract powder, 10-15 parts of compound Chinese herbal medicines, 9-13 parts of a compound plant extract and 3-5 parts of a compound adjuvant. By adding the pig feed additive containing the acremonium terricola culture and the polyrhachis vicina roger extract powder into the basic feed, the utilization rate of the feed can be improved, the weight gain of pigs can be promoted, the content of skatole and indole in pig manure can be remarkably reduced, in practical application, odor emission of a farm can be remarkably reduced, and the economic benefit of the farm is improved. The air quality of the farm and the surrounding of the farm is improved, and the green, low-carbon and sustainable development of animal husbandry can be realized.

The invention relates to the technical field of pig feed, in particular to improved compound feed capable of reducing mutton smell of pork and a preparation method of the improved compound feed. The feed comprises a feed additive with the function of reducing the mutton smell of pork, and the feed additive comprises fructo-oligosaccharide, phytosterol and a salvia miltiorrhiza extract. Through the effects of the fructo-oligosaccharide, the phytosterol and the radix salviae miltiorrhizae extract, the whole-course blocking of chains generated by goaty-flavor substances is realized, and the contents of skatole and androsteneone in fat are remarkably reduced. While the mutton smell is effectively removed, the scheme has no negative influence on the pork quality, and can obviously reduce the drip loss, improve the water holding capacity, reduce the shearing force and improve the red degree of the meat color.

The invention particularly discloses a screening method of microorganisms capable of relieving odor of kitchen waste. The method comprises the following steps: acquiring a sample for enrichment culture; separating and purifying on a specific selective culture medium which respectively takes degradation of amines, hydrogen sulfide and skatole as targets to obtain a single colony; and finally, extracting genome DNA of the strain, amplifying the 16S rRNA gene of the strain, and carrying out sequence alignment, so that the strain is accurately identified. By designing and applying the selective culture medium aiming at the specific odor-causing substances, efficient and directional screening of the target functional strains is realized. According to the method disclosed by the invention, the acinetobacter wuelsii, the acinetobacter baumannii and the bacillus licheniformis are successfully screened and identified, and the strains can be used for effectively degrading main odor-causing substances in the kitchen waste and have a good application prospect.

The invention provides a feed for improving intestinal health of laying hens and a preparation method thereof, and relates to the technical field of feed preparation. The feed is prepared from the following raw materials: corn, yellow powder, soybean meal, peanut kernel cakes, fish meal, calcium hydrophosphate, mountain flour, methionine, vegetable oil, vitamins, table salt, a perilla frutescens extract, a premix, a dichotoma mongolicum leachate and star anise oil. On the basis of basic daily ration, the compound additive taking the dichotoma mongolicum leachate as a component is added, so that the feed cost is reduced, and on the premise of not influencing the egg laying capacity of laying hens and the digestive ability of intestinal tracts, the feed-egg ratio and the quality of eggs are increased, the proliferation of bacteroides cecum is promoted, and the growth of the laying hens is promoted. The abundance of escherichia coli in cecum is reduced, the transfer of odor substances such as skatole and indole generated by microbial metabolism to eggs is reduced, and the market competitiveness of the finished feed is comprehensively improved.

The invention provides a black hair local pig intestine processing and preparation method, and relates to the technical field of food processing. The flash evaporation device used in the aluminum oxide evaporation process comprises a black hair local pig intestine processing and preparation method, and the method comprises the following specific operation steps: S1, raw material pretreatment: raw material pretreatment sequentially comprises the steps of appearance inspection, ultrasonic cleaning biological enzymolysis and supercritical COdegreasing; s2, performing vacuum pulsation marinating; s3, gradient temperature control curing, wherein the gradient temperature control curing process sequentially comprises three stages of swelling, flavoring and shaping; s4, antibacterial and fresh-keeping treatment, wherein the antibacterial and fresh-keeping treatment process comprises edible film spraying and radio frequency pasteurization; and S5, packaging and storing. Through the steps, the skatole removal rate is larger than or equal to 90%, the degreasing rate is larger than 95%, the marinade penetration depth reaches the intestinal wall center, the salinity difference is smaller than 10%, the shrinkage rate is controlled to be smaller than or equal to 22%, the total number of bacterial colonies is reduced by 2 orders of magnitude, heat-resistant bacillus is inactivated, and the sterilization rate is larger than 99.9%.

The invention relates to a method for detecting the presence of skatole in adipose tissue from a male pig, comprising at least the steps of: a) preparing an organic extract from a sample of the adipose tissue; b) subjecting the organic extract prepared in step a) to an electrochemiluminescence reaction; etc.; measuring the intensity of the luminescence during step b) and, if the measured luminescence intensity exceeds a predetermined threshold value, inferring the presence of skatole in the adipose tissue sample; and characterized in that step a) comprises the substeps of: i) collecting the adipose tissue sample using a sampling swab; ii) dissolving the adipose tissue sample in a medium comprising an aprotic organic solvent; iii) adding an anhydrous background salt to the aprotic organic solvent, this addition being able to be carried out before or after substep ii).Applications: identification, on slaughter lines, of entire male pigs whose meat carries boar odor; research tool, in particular to study the factors influencing the production of skatole in the adipose tissues of entire male pigs with a view to developing methods to prevent or reduce boar odor.

The invention relates to lactobacillus plantarum CTZ021 for promoting intestinal health of pets and application of the lactobacillus plantarum CTZ021, and belongs to the technical field of microorganisms. The preservation number of the strain is CCTCCNO: M20251255. The feed additive has good acid and cholate resistance, excellent gastrointestinal survival ability and definite probiotic efficacy. By adjusting the balance of intestinal flora, the production of harmful metabolites (skatole and indole) is effectively inhibited, the odor of excrement is fundamentally improved, and the intestinal health is reflected; healthy growth of pets is promoted by enhancing digestion and absorption, supporting immunity and reinforcing a barrier; by maintaining flora stability, strengthening a barrier and relieving inflammation, diarrhea and intestinal injury caused by diarrhea are effectively prevented and improved. Due to the characteristics, the strain becomes an excellent strain with great potential for developing efficient and safe special probiotic preparations for pets and daily maintenance type functional foods.