164 results about "Putrescine" patented technology

The invention relates to the field of biology, and discloses a serum-free culture medium which essentially comprises an IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, Hepes, recombinant human insulin, recombinant human transferrin, recombinant human albumin, 2-mercaptoethanol, protocatechuic acid, lipid, amino acid, vitamins, trace elements, Pluronic F-68, hydrocortisone, vitamin C, bonding amine or recombinant human fibronectin, progesterone, putrescine, heparin, serotonin, epidermal growth factors (EGFs), b-fibroblast growth factors (FGF), platelet derive growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I. The serum-free culture medium is clear in chemical components, free from animal sources and serum and safe and ideal in cell cultivation and avoids the doped animal components and unstable batches, and the results of the cultured mesenchymal stem cells show that the total cellular score, the cell phenotype and the secretory cell factors are normal, so that the serum-free culture medium has good industrial application prospect.

The present invention belongs to the field of biotechnology, and discloses a serum-free culture medium with specific chemical compositions for in vitro culture and amplification of bone marrow mesenchymal stem cells. By adding insulin, transferrin, ethanolamine, sodium selenite, growth factors, adherent factors, hormone, putrescine, inorganic salt, vitamin, albumin and antioxidant into a basic culture medium, the bone marrow mesenchymal stem cells can attach to the culture medium under a serum free condition, so the in vitro culture and amplification are realized, the potential of multi-directional differentiation is maintained, and the amplified cells can be induced to be osteoblast and lipocyte in vitro. The serum-free culture medium has the advantages that the clinic level cell products for human produced by the serum free culture medium can effectively avoid the potential risk of producing cell products by serum culture medium. The drawing appended is a photo of the confluence of the bone marrow mesenchymal stem cells cultured by the serum-free culture medium.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

Compositions and methods for isolating and expanding human mesenchymal stem/progenitor cells through multiple passages in defined serum-free environments are provided. The culture media compositions includes a basal medium supplemented with a nutrient mixture such as Ham's F12 nutrient mixture, glutamine, buffer solutions such as sodium bicarbonate and hepes, serum albumin, a lipid mixture, insulin, transferrin, putrescine, progesterone, fetuin, hydrocortisone, ascorbic acid or its analogues such as ascorbic acid-2-phosphate, fibroblast growth factor and transforming growth factor β, and are free of serum or other undefined serum substitutes such as platelet lysate. Methods employing these compositions and protein-coated surfaces for the isolation of mesenchymal stem/progenitor cells from human bone marrow and other tissues such as adipose tissue are also provided. Finally, methods are also provided for serially expanding these cells through multiple passages without losing mesenchymal stem cell-specific proliferative, phenotypical and differentiation characteristics.

The invention relates to an SFM (serum-free medium) for culturing MSCs (mesenchymal stem cells). Based on volume, the SFM comprises the following components: 10.2 grams per liter of alpha-MEM (alpha-minimum essential medium), 2.4 grams per liter of sodium bicarbonate, 1 to 5 millimoles of L-glutamine, 50 to 300 milligrams per liter of poloxamer 188, 2 to 8 grams per liter of recombinant human albumin, 10 to 20 milligrams per liter of recombinant human transferrin, 2 to 10 milligrams per liter of recombinant human insulin, 1 to 5 millimoles per liter of Hepes, 50 nanomoles of beta-mercaptoethanol, 0.1 to 1 milligram per liter of lipid, 1 to 5 milligrams per liter of trace element, 0.1 to 5 milligrams per liter of glutathione, 0.5 to 5 milligrams per liter of para-aminobenzoic acid, 1 to 50 nanograms per milliliter of hydrocortisone, 20 to 50 milligrams per liter of vitamin PP, 5 to 50 milligrams per liter of vitamin C, 2 to 10mu M of compound shown in a formula I, 5 to 20mu M of compound shown in a formula II, 10 to 20 nanograms per milliliter of progestin, 1 to 10 milligrams per liter of putrescine, 1 to 10 international units per liter of heparin, 1 to 10 nanograms per milliliter of EGF (epidermal growth factor), 1 to 10 nanograms per milliliter of b-FGF (b-fibroblast growth factor), 1 to 10 nanograms per milliliter of HGF (hepatocyte growth factor) and 1 to 10 nanograms per milliliter of VEGF (vascular endothelial growth factor). The SFM for culturing the MSCs is a BPS-SFM which has determinate chemical components and is free of animal-derived substances.

The present invention relates to a preparation method of 4-[3-(2-butyl-amino) propoxy]- benzoic alkyl ester (a compound of formula (1)). The p-hydroxide benzene methyl formate and 1, 3 methylparaben react to prepare compound III under alkaline conditions. Then the products and putrescine dock to synthesize compound I under alkaline conditions. The compound is a key intermediate for preparing drugs of treating arrhythmic diseases, hydrochloride Juenaidalong. Wherein, R represents C₁-C₄ alkyl; X, X1 and X2 are halogen atoms, such as Cl, Br and I; but X₁ and X₂ are different from each other.

Nutrient compositions and methods that sustain and promote positive metabolic energy levels in a targeted manner are disclosed. Methods utilize endogenous energy stores (fat oxidation), increase use of those stores (increasing transport rate), increase available energy (increasing the ability to perform ADP to ATP phosphorylation,) as well as decrease catabolism and increase protein synthesis. Compositions are also disclosed, and include Mono- or Dicreatine-HMB salt; Putrescine Dihydrochloride; Alanine; L-Glutamine, which may be combined with Alanine in a 1:2 to 2:1 molecular ratio; Trimethylglycine; and Guanidinopropionic Acid.

The invention discloses a cold-resistant agent for growth of Chinese trichosanthes. The cold-resistant agent comprises the following components by weight: 5-7.5mg of abscisic acid, 0.03-0.06g of salicylic acid, 0.6-1g of calcium nitrate, 0.035-0.04g of putrescine, 0.05g of spermidine, 0.45g of urea, 0.3g of monopotassium phosphate, 0.04g of citric acid, 0.1g of malic acid, 0.05g of chitosan, and 1L of water. The invention simultaneously discloses a using method of the cold-resistant agent for growth of Chinese trichosanthes. The method comprises the following steps of: spraying leaves of a Chinese trichosanthes plant by a Chinese trichosanthes cold-resistant agent until the leaves drip 12-48 hours before a cold wave comes. By adopting the cold-resistant agent for growth of the Chinese trichosanthes disclosed by the invention, the ability of the Chinese trichosanthes for resisting low-temperature damage can be obviously enhanced; and the stability of a Chinese trichosanthes cell membrane also can be enhanced.

The invention discloses a blood serum-free culture medium suitable for the suspension culture of CHO cells with large scale and high density. The culture medium takes DMEM/F12 (v/v, 1:1) as a base culture medium, and adds the other substances such as insulin, putrescine, transferrin and microelement, etc. The blood serum-free culture medium has the advantages that: the culture medium can support the CHO cells to fast growth under the condition of suspension culture; the protein content is very low, and the chemical composition is basically clear; the cells are grown one by one by means of suspension, so that the living cell density and the cell living rate are higher than those of the serum culture and the similar commercialized serum-free culture; and the cost is lower.

The invention discloses a lactobacillus plantarum and its application in the high-acid yellow wine production for acid modulation, belonging to the field of rice wine brewing technology. The lactobacillus plantarum CGMCC No.12757, isolated from rice wine fermented mash. The lactobacillus plantarum comprises of total acid of rice wine produced of more than 17.5g/L (in lactic acid), lactic acid content of 70% or more accounting for the total organic acid, 14% (v / v) or more of alcohol content, 52mg / L biogenic amine content (the total amount of putrescine, histamine and tyramine), far lower than the rancid rice wine in the storage process, which can produce high-acid rice wine for acid modulation, greatly facilitating the production of low-grade rice wine.

The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.

Belonging to the field of biotechnology and fermentation, the invention discloses a method for degradation of biogenic amines in wine making by lactobacillus plantarum. The strain is obtained by screening the surfaces of wine grapes, not only can effectively reduce the content of biogenic amines in an MRS medium added with putrescine, histamine and tyramine, but also can be directly applied in the wine making process. With good high acid and high alcohol content and high SO2 tolerance, and very high malic acid-lactic acid conversion rate, the strain can effectively decrease the content of biogenic amines in the wine making process.

The invention provides berberine-containing serum-free medium for mesenchymal stem cells, comprising a DMEM basic medium and further comprising, by concentration, 5-110mg/L berberine, 3-20mg/ml basic fibroblast growth factors, 3-20mg/ml epidermal growth factors, 1-10Mug/mL glutathione, 1-10mg/ml recombinant human insulin, 5-15mg/ml human serum albumin, 1-10Mug/mL transferrin, 5-15mg/L fibronectin, 5-30mg/L putrescine, 0.37-4mg/L aminoethanol, 10-50Mug/mL hydrocortisone, and 0.02-0.2mg/ml sodium pyruvate. The invention belongs to the technical field of stem cells; the medium has good cell adherence, high cell proliferation rate and capable of delaying decline phase of MSCs (mesenchymal stem cells) and prolong growth period of the cells.

The invention discloses dendritic flame-retardant layered silicate and a preparation method thereof, and the preparation method comprises the following steps: (1) mixing inorganic montmorillonite and high heat-resistant organic intercalator, adding ethanol water solution, stirring for 2-4h at 75-80DEG C and collecting high heat-resistant organic montmorillonite; (2) stirring the high heat-resistant organic montmorillonite, putrescine, acrylonitrile and catalyst for 5-8h at 70-90DEG C, and then collecting dendritic organic montmorillonite from the product; and (3) stirring the dendritic organic montmorillonite and alpha-(diphenylphosphino) acetic acid for 1-2h at 60-80DEG C, then adding zinc borate, and stirring for 2-4h at 70-90DEG C, thus obtaining the dendritic flame-retardant layered silicate. The dendritic flame-retardant layered silicate is applicable to rubber industry and cable industry, which can not only replace the traditional environmental-unfriendly flame-retardant and reinforcing material of high price, but also improve the performance of rubber material, such as flame resistance, mechanical properties and the like.

The invention provides a method for synthesizing chlorinated formic acid 9-fluoren methyl, which comprises the following steps: (1) 9-fluoren methyl and triphosgene are reacted for 0.5 to 3 hours in ice bath, under the existence of catalyst and in organic solvent; the catalyst is chosen from N with nucleophilicity, N-dimethylformamide, acetone, diisopropyl ethyl amine, diethylamine, triethylamine, ethyleendiamine, putrescine, imidazole, and the solvent is chosen from benzene, toluene, cyclohexane, hexane, heptane, cyclopentane, dichloromethane, chloroform and the compound thereof; (2) the temperature raises to 20 to 50 DEG C, and the mixture reacts for 1 to 5 hours. The method for synthesizing chlorinated formic acid 9-fluoren methyl adopts safe solid phosgene as reactant, and is simple in technique, convenient in safety, mild in condition, high in yield and easy in realization of industrialization production; the solvent can be used repeatedly after being processed simply, and the products do not need to be refined, and purity is up to more than 98 percent.

The invention discloses a Chinese hamster ovary culture medium as well as a preparation method and application thereof. The culture medium comprises ascorbic acid, pyridoxine hydrochloride, vitamin B12, nicotinamide, riboflavin, thiamine chloride, choline chloride, folic acid, calcium pantothenate, sodium pyruvate, reduced glutathione, inositol, biotin, hypoxanthine, putrescine, lipoic acid, linoleic acid, thymine, glucose, HEPES (2-hydroxyethyl) buffer solution, dextran sulphate, Pluronic-F68, various amino acids and salts thereof and various inorganic salts. The culture medium has low cost, and the component does not contain animal origin ingredients, does not contain protein and has definite chemical ingredients. All ingredients are cooperated and blended, and the Chinese hamster ovary culture medium can satisfy the basic growth requirement of cells, and can effectively regulate, transfer and control the culture of cells so as to realize good high-density cell culture. Compared with the culture effect of the existing culture medium, the culture effect of culture medium disclosed in the invention is at least equivalent to even superior to the existing culture medium.

The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.

The invention provides a serum-free, animal-derived-component-free culture medium with definite components and application thereof, and relates to the technical field of cell culture. The serum-free and animal-derived-component-free culture medium with definite components comprises a basic culture medium and cytokines, hormones, transferrin, albumin, fetal globulin, putrescine and serotonin whichare added into the basic culture medium. According to the serum-free, animal-derived-component-free culture medium with definite components, substances such as cytokines, hormones and the like are added into a basic culture medium to replace serum, so that the culture of human cells is realized. The components of the culture medium are definite, no serum and animal-derived-component components areadded, so that the technical problems of poor repeatability of the traditional serum-containing culture medium for cell culture, low cell activity and low cell amplification efficiency of other serum-free culture mediums can be mitigated.

The invention particularly discloses an insect attractant capable of improving the pollination rate and the fruit set percentage of a jujube tree, and application of the insect attractant. The insectattractant comprises a nutrient solution and an animal and vegetable decomposition liquid, wherein the nutrient solution comprises the following ingredients by weight percent: 27.56-38.38% of honey, 0.01-0.09% of putrescine, 0.01-0.09% of spermidine, 0.1-1.3% of salicylic acid, 0.1-0.5% of gibberellin and 60.36-71.5% of urea; the animal and vegetable decomposition liquid is a supernate obtained byfiltering after fermentation of egg, cooked beans and chicken entrails for 7-8 days at 25-35 DEG C; and the nutrient solution, the animal and vegetable decomposition liquid and water are compounded at the proportion of 3:1:16. By spraying the insect attractant onto jujube flowers in the flowering stage, the proportion of fly flower-visiting insects in the total flower-visiting insect number is averagely increased by 5-8 percent. The insect attractant can be sprayed to achieve a remarkable trapping effect on flies and bees, the number and the flower-visiting frequency of flower-visiting insects are increased in a unit time, and the pollination and fertilization rates of jujube flowers are increased. After spraying of 100 times and 200 times of the insect attractant, the single-plant yieldsof the jujube tree are increased by 56.94% and 35.08% respectively, the prune single-plant yields are increased b6 36.36% and 25.38% respectively, and the almond single-plant yields are increased by48.11% and 44,30% respectively.

The invention relates to the field of cell culture medium, and in particular to a non-serum non-animal-origin-additive insect cell culture medium. The medium comprises mainly the following components: basic culture medium, glucose, inorganic salt, vitamins, L-arginine, L-agedoite, L-glycocoll, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine, L-proline, L-glutamine, yeast extracts, recombulin, malic acid, allomaleic acid, cholesterol, linoleic acid, granulesten, putrescine, glutathione, glycerol and fructosan. The culture medium can promote the growth of insect cell and is suitable for the large scale breeding of insect cell and the expression of recombination protein.

The invention provides a polyamine composite agent enhancing wheat and corn drought resistance. The composite agent is composed of the following components, by weight: 7-280 parts of putrescine, 0.4-10 parts of spermidine, 0.8-16 parts of spermine, 1-15 parts of succinic acid, and 6-150 parts of chitin. According to the invention, the weighed components are dissolved into 50000-150000 parts of water, such that the polyamine composite agent is obtained. Compared with prior arts, formerly commonly used spraying agent components are broken through. While the drought resistance of wheat and corn is improved, polyamine can be absorbed by plants according to agriculturally applied concentrations, such that no residue is left in soil. Even the polyamine absorbed by plants enters human and livestock bodies, the concentrations are greatly lower than those synthesized by human and livestock. The composite agent is harmless to human and livestock, and has a relatively low cost.

The invention provides a serum substitution combination for immune killer cell amplification in vitro. Insulin with the final concentration being 0.5-20 mg/L, transferrin with the final concentration being 0.69-22 mg/L, bovine serum albumin with the final concentration being 3000-6000 mg/L, ethanolamine with the final concentration being 0.61-2.44 mg/L, alpha-thioglycerol with the final concentration being 5.41-10.82 mg/L, linolenic acid with the final concentration being 0.5-5.0 mg/L and cholesterol with the final concentration being 0.8-20 mg/L are added into a conventional culture medium. In the serum substitution combination, ferric ammonium citrate with the concentration being 0.46-2.3 mg/L, beta-disodium glycerophosphate with the concentration being 300-1500 mg/L and putrescine with the concentration being 0.25-1.25 mg/L can be added. The components are definite, can be added into different culture media such as an RPMI1640 culture medium, a DMEM/F12 culture medium and an IMDM culture medium, can support immune killer cell amplification in vitro, and are efficient and universal. In vitro serum-free amplification of immune killer cells can be achieved, the biological therapy of tumors is promoted, and conduction of the somatic cell therapy is promoted particularly.