35 results about "Putrescine" patented technology

The invention is applicable to the technical field of molecular biology, and provides a mutant gene, a mutant, a transformant and a genetic transformation plant of tobacco deoxycarboxy-putrescine lysine synthase and application of the mutant gene, the mutant, the transformant and the genetic transformation plant of the tobacco deoxycarboxy-putrescine lysine synthase of the tobacco deoxycarboxy-putrescine lysine synthase of the tobacco deoxycarboxy-putrescine lysine synthase. The nucleotide sequence of the mutant gene is shown as SEQ ID NO.2 in a sequence table. After the gene is mutated, a mutant strain shows the phenotype of white veins, and various nutrients are greatly changed. According to the mutant gene of the tobacco deoxycarboxamide lysine synthase provided by the invention, by detecting the mutant and comparing the potassium content in tobacco leaves, the result shows that the mutant gene of the tobacco deoxycarboxamide lysine synthase can improve the potassium content in the tobacco leaves. Therefore, a better choice is provided for genetic improvement of new plant varieties, and the gene has important application value.

The application discloses a method for detecting plant free polyamines by HPLC, which can clearly separate the target substances putrescine (Put), spermidine (Spd), thermospermine (T-Spm) and spermine (Spm) in a plant sample. The method has stable retention time of the separated target substances, and the peaks corresponding to the target substances separated from different samples and the standard sample can be well overlapped, data is clear, and the result is reliable. The separation time is shortened by half, the use amount of the mobile phase is halved, the operation cost is greatly reduced, the waste liquid is halved, the recovery cost of the waste liquid is also halved, and the potential pollution to the environment is reduced.

The invention discloses a composition for promoting bulb expansion of hippeastrum hippeastrum, the composition is an aqueous solution, the solute in the composition comprises putrescine, spermidine, spermine, triacontanol, ethanol, Tween 80, sodium lignin sulfonate and ethylene glycol, and the dosage ratio of the materials is 10: 1: 2: 0.05: 10: 5: 3: 2 according to the dosage weight. And diluting the composition with water to obtain a diluent with the mass percent concentration of 0.04-0.06%. The diluent is used for foliage spraying and seedball base irrigation in the germination stage, the growth stage, the flowering stage and the post-flowering rejuvenation stage of hippeastrum hippeastrum, the seedball diameter of hippeastrum hippeastrum can be remarkably increased, and economic benefits are increased.

The invention belongs to the technical field of tea tree gene application, and particularly relates to application of a tea tree CsPHT gene in improving tea geometrid and colletotrichum gloeosporioides resistance of a tea tree. The invention discloses a tea tree CsPHT gene, the nucleotide sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.1, the tea tree CsPHT gene codes 434 amino acid residues, and the amino acid sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.2. The CsPHT gene is proved to be capable of catalyzing biosynthesis of tea tree defense related compounds such as feruloyl putrescine, coumaroyl putrescine and cinnamoyl putrescine for the first time, and the conservation of the catalytic function of the CsPHT gene is proved through heterologous expression. An in-vivo instantaneous function verification result of a tea tree shows that the over-expression of the CsPHT gene can significantly improve the resistance of the tea tree to tea geometrid and tea anthracnose bacteria. The CsPHT gene is proved to be a key positive regulation factor for regulating the resistance of the tea tree to the tea geometrid and the tea anthracnose fungus. The invention provides important gene resources and molecular targets for cultivating new varieties of high-resistance and multi-resistance tea trees.

The invention discloses a dual-mode sensor for detecting putrescine in food as well as a preparation method and application of the dual-mode sensor. The dual-mode sensor (CDs / AuNCs) is composed of carbon dots (CDs) and gold nanoclusters (AuNCs), and putrescine can be rapidly and sensitively detected within 20 seconds. The interaction of putrescine and the sensor results in an increase in the fluorescence intensity of CDs at 430 nm and 525 nm, which is caused by the aggregation-induced emission (AIE) effect of CDs and the internal filter effect (IFE) of AuNCs. The detection limit of the CDs / AuNCs to putrescine in a ratio fluorescence mode is as low as 0.44 mu M, and the CDs / AuNCs has excellent selectivity and anti-interference performance. CDs / AuNCs also show good colorimetric linear response to putrescine, and the detection limit is 0.72 mu M. Finally, the CDs / AuNCs realizes rapid and accurate detection of putrescine in prawn meat by means of a smart phone, and shows the potential of food spoilage detection.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.

A culture medium for immune cells and use thereof. The culture medium comprises: 200-1000 U / mL IL-2, 10-100 ng / mL IL-4, 10-100 ng / mL IL-15, 2-20 ng / mL anti-CD3 monoclonal antibody, 2-20 ng / mL anti-CD28 monoclonal antibody, 0.5-20 μM GSK-3β inhibitor, 0.1-10 μM Wnt / β-catenin signaling pathway agonist, 1-10 μg / mL transferrin, 1-10 μg / mL ethanolamine, 1-10 μg / mL water-soluble cholesterol, 1-10 μg / mL insulin, 1-10 mM L-alanyl-L-glutamine, 1-10 μg / mL L-ascorbic acid, 1-10 μg / mL taurine, 0.1-1 μg / mL putrescine, and an RPMI 1640 medium. The culture medium can effectively keep the survival rate of cells, efficiently induce the proliferation of memory T cells, and amplify the number of memory T cells. The culture medium has the advantages of low cell culture cost and good cell quality.

The invention discloses a huperzia serrata HsCAO1b gene and application thereof, and belongs to the technical field of molecular biology and genetic engineering. Aiming at the problems that huperzia serrata wild resources are scarce, and CAO family genes of the huperzia serrata have unknown functions and are difficult to clone, the invention provides a complete coding sequence SEQ ID NO: 1 of the gene, constructs an expression vector containing the gene, and introduces the expression vector into host cells and plants. The gene can be used for regulating the metabolism of amine substances in plants, reducing the content of 2-phenylethylamine, piperidine or putrescine and increasing the content of spermidine or spermine; alkaloid synthesis is regulated and controlled, and total alkaloid is reduced or nicotine content is increased; the quantity of rosette leaves in the bolting period is increased to regulate and control plant growth and development, and gene resources are provided for production of active ingredients such as huperzine A and the like.

The invention provides a microalgae culture medium and a microalgae culture method, and belongs to the technical field of algae culture, the microalgae culture medium provided by the invention comprises a nitrogen source and a salt agent, the mass ratio of the nitrogen source to the salt agent is 1: (0.7-1.3), and the nitrogen source comprises a phytic acid compound, amino acid and putrescine. When the microalgae culture medium provided by the invention is used for culturing microalgae, the growth rate, the total lipid accumulation rate and the unsaturated fatty acid content of the microalgae can be effectively improved, and the microalgae culture medium provided by the invention is not easy to cause eutrophication of a water body and is suitable for large-scale culture of the microalgae.

The application discloses a kind of meat freshness rapid test paper, belong to food rapid detection technical field.The test paper is with water absorption filter paper as base material, and is loaded by the reaction color developing system consisting of o-Phthaldehyde, mercapto glycolic acid, sodium acetate buffer and fast blue B salt, can occur specific color reaction with the biological amine (putrescine) generated in the meat spoilage process, and realizes freshness rapid judgment by color ladder change.The detection time of the application is only 3-5 minutes, without large instrument, simple operation, high sensitivity, low cost, wide spectrum, can be used for on-site rapid screening of pork, beef, mutton, frozen shrimp, frozen chicken breast and other various meats, and is widely applicable to raw material acceptance of meat processing enterprises, market supervision law enforcement and cold chain logistics quality monitoring.

The invention discloses two plant growth regulation products for increasing the yield of carya illinoensis, and a preparation method and an application method of the two products. The first plant growth regulation product is prepared from 0.5 to 1.5 mmol / L of putrescine, 0.05 to 0.15 mmol / L of spermidine and 40 to 60 mg / L of cytokinin; and the second plant growth regulation product is prepared from 0.5 to 1.5 mmol / L of putrescine, 0.05 to 0.15 mmol / L of spermidine and 400 to 600 mg / L of cytokinin. The first plant growth regulation product and the second plant growth regulation product both can increase the number of female flowers, relieve the fruit drop condition in the development period and improve the fruit setting rate, and have positive influences on the yield increase of the carya illinoensis. The plant growth regulating product provided by the invention is simple in preparation method, simple in application method, easy to operate, high in practicability, capable of meeting the industrial development of large-scale cultivation and production of the carya illinoensis and wide in application prospect.

The invention discloses application of putrescine as a molecular marker in preparation of a reagent for evaluating the severity of alcohol use disorder. The reagent is used for detecting the content of putrescine in a sample. By detecting the putrescine content in the excrement sample of the tested person, the alcohol use disorder severity of the person can be evaluated, and the method has important clinical significance for formulating a better diagnosis and treatment scheme.

The invention relates to a trace hydroxyputrescine lysine protein detection method, which comprises: 1) preparing a sample to be detected, and slicing cells, body fluid or tissue; (2) sealing the sample by using Duolink sealing liquid; (3) diluting an anti-rabbit PLUS probe and an anti-mouse MINUS probe Merck and DUO92101 / DUO92102 in proportion by using a Duolinkantibody diluent, incubating with a product obtained in the previous step, and respectively combining a rabbit polyclonal antibody and a mouse monoclonal antibody through the PLUS / MINUS probe to provide a basis for a ligation reaction; (4) diluting ligase by using a Duolink ligation buffer solution according to the ratio, and incubating with the product in the step (3) at the temperature; 5) diluting an amplification enzyme with a Duolink amplification buffer solution in proportion, and incubating with a product obtained in the previous step; 6, a mounting medium containing DAPI is adopted for mounting, and imaging analysis is carried out through a fluorescence or confocal microscopy. The problem that due to the fact that the affinity of the antibody is insufficient and the protein expression quantity is low, the omission ratio of the trace hydroxyputrescine lysine modified protein is high can be well solved.

The present application belongs to the field of wheat growth regulation, and particularly relates to a wheat growth regulation method. In view of the technical problem of negative correlation between the growth cycle and yield of wheat, a new method for cultivating wheat is provided. The present application sprays putrescine on the wheat ear, which can effectively shorten the growth cycle of wheat while ensuring the stability of effective tiller number, thousand-grain weight and ear grain number, and avoiding yield loss. The mechanism of action of putrescine may match the endogenous putrescine metabolism characteristics of wheat, realize the synergy of growth regulation and yield maintenance, and effectively improve the protein content.

The invention relates to the field of biological pharmacy, in particular to a composition for cell-free protein synthesis and application thereof. The composition for cell-free protein synthesis comprises multiple of the following components: ribonucleic acid, folic acid, oxalic acid, putrescine, spermidine, potassium salt, magnesium salt, ammonium salt, amino acid, PEG8000 and a cell extract. According to the preparation method of the antibody coupling medicine, the raw materials are simple and easy to obtain, the universality is high, and the problems that in a traditional method, a full-length antibody has four pairs of disulfide bonds which are conveniently used for fixed-point coupling, and a single-domain antibody has no exposed disulfide bonds and is not prone to fixed-point coupling can be solved; therefore, the preparation method of the antibody coupling medicine can easily obtain the single-domain antibody coupling medicine with fixed-point coupling, good uniformity and various DAR values.

A method for ratiometric detection of putrescine using PtPd NPs@TPE fluorescent nanozymes. This invention first utilizes mesoporous platinum-palladium nanoparticles (PtPd NPs) doped with tetraphenylethylene (TPE) to obtain fluorescent nanoparticles (PtPd NPs@TPE) with oxidase-like activity, which can produce blue fluorescence under 365 nm ultraviolet light excitation. Secondly, this invention utilizes the antioxidant properties of putrescine solution to inhibit the formation of 2,3-diaminophenazine (DAP), an oxidation product, during the oxidation of o-phenylenediamine (OPD) by PtPd NPs@TPE, thereby reducing the DAP content. Based on the relationship between putrescine concentration and the colorimetric intensity of DAP, and the ratio of the yellow fluorescence intensity of DAP at 552 nm to the blue fluorescence intensity of PtPd NPs@TPE at 442 nm, a novel ratiometric fluorescence method for detecting putrescine is constructed. This method can be used to detect actual samples such as dried bean curd, with a detection limit as low as 0.0025 μg / mL.

This invention belongs to the fields of bioinformatics and medical testing technology, specifically relating to a combination of biomarkers related to the postoperative prognosis of colorectal cancer and their application and prediction system. This invention, through 16S rRNA gene sequencing of tumor mucosal samples from colorectal cancer patients, combined with long-term postoperative follow-up data and screening, found that *Peptostreptococcus*, *Fusobacterium*, *Bacteroides*, *Porphyromonas*, and *Prevotella* are closely related to postoperative recurrence of colorectal cancer, with an AUC of 0.81 for assessing postoperative recurrence. Based on this, this invention conducts non-targeted metabolomics analysis, finding that combining alanine glutamate, putrescine, arginine, histidine, and sebacic acid with the above microbial biomarkers can improve the AUC to 0.91, significantly enhancing accuracy, specificity, and precision. This enables prognostic stratification of recurrence-free survival in colorectal cancer patients, demonstrating higher predictive efficacy and stability.

To provide a method capable of conveniently and accurately determining stress.SOLUTION: The present invention provides a method for acquiring stress determination data, the method including measuring a stress determination marker that includes at least one selected from pyruvic acid, nicotinamide, Ile, N-acetyl-β-alanine, 2AB, choline, Val, uracil, xanthine, estrone, inosine, adenosine, and Leu in a biological sample derived from a subject, or includes a combination of at least one selected from propionic acid, spermine, 3-methylbutanoic acid, 4-methyl-2-oxopentanoic acid, diethanolamine, putrescine, and body temperature together with at least one of the above.SELECTED DRAWING: Figure 1

To provide an improved serum-free animal cell culture medium that can be used for production of a protein of interest.SOLUTION: Inclusion of ornithine, either with or without putrescine, in serum-free cell culture medium ("OS" medium) increases cell viability and density, decreases cell doubling time, and allows for high titer protein production by these cells.SELECTED DRAWING: None

The application is a divisional application of "ornithine decarboxylase mutant, gene, recombinant plasmid, recombinant strain and application", relates to the technical field of genetic engineering and enzyme engineering, and particularly relates to an ornithine decarboxylase mutant, gene, recombinant plasmid, recombinant strain and application. The ornithine decarboxylase mutant is obtained by mutating a wild-type ornithine decarboxylase with an amino acid sequence shown in SEQ ID NO. 1, and the mutation site of the ornithine decarboxylase mutant is H137R. The ornithine decarboxylase mutant with high enzyme activity is obtained by single-point mutation or multi-site combined mutation of the wild-type ornithine decarboxylase, so that the substrate conversion rate of the ornithine decarboxylation reaction and the synthesis efficiency of putrescine are significantly improved, the rate-limiting bottleneck of the ornithine decarboxylation reaction in the synthesis path from glucose to ornithine and then to putrescine is successfully broken through, and the overall efficiency of the metabolic pathway is significantly optimized.

A biomarker panel for a serum test for detecting lung cancer, wherein the biomarker is selected from the group consisting of arginine, C18.2, decadienoylcarnitine (C10:2), LYSOC18.2, methionine, ornithine, PC32:2AA, PC36.0AA, PC36.0AE, putrescine, spermidine, spermine, and valine. Serum testing for diagnosing lung cancer may take into account smoking history.

This invention discloses a method for simultaneously and accurately determining the content of multiple biogenic amines in baijiu (Chinese liquor). Based on gas chromatography-triple quadrupole mass spectrometry (GC-MS), it detects multiple biogenic amines in baijiu, including nicotinamide, β-phenylethylamine, guanidine, and putrescine. In the pretreatment process, 1,7-diaminoheptane is added as an internal standard, and a mixture of N,O-bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane is used as a derivatization reagent, effectively simplifying the pretreatment process. By controlling the conditions of GC and MS, accurate, rapid, and highly sensitive determination of multiple biogenic amines in baijiu is achieved. In this invention, baijiu samples do not require extraction with organic solvents or pH adjustment; the pretreatment operation is extremely simple, and the sensitivity and quantitative accuracy of biogenic amines are high, with good stability and reliability. This effectively improves the problem of complex pretreatment operations and poor detection accuracy in the quantitative determination of biogenic amines in baijiu.

The invention belongs to the technical field of bioengineering, and relates to application of feruloyl putrescine in preparation of deoxynivalenol. The invention verifies that when the feruloyl putrescine is used as the fusarium graminearum culture medium additive, the fusarium graminearum can be promoted to generate deoxynivalenol. According to the invention, more ways are provided for synthesis and acquisition of deoxynivalenol.

This invention belongs to the field of bioengineering technology and relates to the application of ferulic acid putrescine in the preparation of deoxynivalenol. This invention verifies that ferulic acid putrescine, when used as an additive in the culture medium of *Fusarium graminearum*, can promote the production of deoxynivalenol by *Fusarium graminearum*. This invention provides more pathways for the synthesis and acquisition of deoxynivalenol.

The present invention generally related to a modified microbial cell capable of producing high levels of spermidine and / spermidine derivatives. The genetically modified microbial cell comprises at least one modification to native spermidine biosynthetic pathway via putrescine together with genes involved in the S-adenosylmethionine biosyntheticpathway.

The invention belongs to the technical field of marine organisms, and particularly relates to a method for qualitatively and quantitatively detecting thallus and protonema polyamines of macroalgae laver species by utilizing an extracting solution in combination with LC-ELSD (liquid chromatography-evaporative light scattering detector), which comprises the following specific processes: 1) preparing a disodium hydrogen phosphate-citric acid extracting solution with the pH value of 3-6; (2) adding thalli and protonema of laver species into an acidic extraction solution, crushing by using a cell crusher, centrifugally extracting supernate, carrying out high-temperature overnight acidolysis on algal residues by using an acid solution, and centrifugally extracting supernate; the extracted supernate is used for LC-ELSD (liquid chromatography-evaporative light scattering detector) detection; and 3) LC-ELSD qualitative and quantitative detection: according to the appearance time of putrescine, spermidine, spermine and hot spermine standard substances and the established standard curve, qualitatively and quantitatively detecting the phyllosome and protonema polyamine components of the laver species.

The present invention relates to a composition for preventing or treating diabetes mellitus using a 4-component mixture of putrescine, glucosamine, nicotinamide, and a STAT3 inhibitor. It has been confirmed that blood glucose is stably regulated when a compound of the 4-component mixture according to the present invention is injected into the caudal vein of a diabetes mellitus-induced mouse. The compound can be practically and usefully used as a therapeutic agent for diabetes mellitus patients and patients exposed to diabetes mellitus risk.

The invention discloses a method for fermenting smelly mandarin fish by using potassium chloride to replace salt and inoculate bacteria, and belongs to the technical field of food fermentation processing. Potassium chloride is used for replacing part of sodium chloride, and the content of sodium chloride in the smelly mandarin fish can be remarkably reduced. Potassium chloride is added to replace sodium chloride, the metabolic activity of lactic acid bacteria in the inoculation fermentation process is effectively adjusted, the growth activity of microorganisms such as putrefying bacteria is inhibited, and the number of enterococcus, pseudomonas and hydrogen sulfide producing bacteria in each gram of fish meat in the fermentation process is obviously reduced. Potassium chloride is added to replace sodium chloride, accumulation of volatile basic nitrogen and biogenic amine in the fermentation process is inhibited, the volatile basic nitrogen is as low as 28.87 mg / 100g, putrescine is 137.45 mg / Kg, and tyramine is 18.34 mg / Kg.

The present invention discloses a mutant of Escherichia coli putrescine aminotransferase and its application, belonging to the field of bioengineering technology. The present invention overcomes the limitation of low catalytic stability of the wild-type enzyme by mutating the 91st phenylalanine to tyrosine in the putrescine aminotransferase PaTA derived from Escherichia coli, and the obtained mutant F91Y has an enzyme activity of 2.56U / mg. The recombinant bacteria expressing the mutant F91Y use 10g / L 1,4-cyclohexanedicarboxaldehyde as a substrate and the conversion rate reaches 50.48%. It has high production intensity and high catalytic stability in the preparation of 1,4-cyclohexanedimethylamine, reduces the amount of bacteria used in the conversion, and greatly saves the production cost of industrialization.

The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and / or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and / or putrescine or related products using the microbial organisms.