167 results about "Putrescine" patented technology

The invention relates to the field of biology, and discloses a serum-free culture medium which essentially comprises an IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, Hepes, recombinant human insulin, recombinant human transferrin, recombinant human albumin, 2-mercaptoethanol, protocatechuic acid, lipid, amino acid, vitamins, trace elements, Pluronic F-68, hydrocortisone, vitamin C, bonding amine or recombinant human fibronectin, progesterone, putrescine, heparin, serotonin, epidermal growth factors (EGFs), b-fibroblast growth factors (FGF), platelet derive growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I. The serum-free culture medium is clear in chemical components, free from animal sources and serum and safe and ideal in cell cultivation and avoids the doped animal components and unstable batches, and the results of the cultured mesenchymal stem cells show that the total cellular score, the cell phenotype and the secretory cell factors are normal, so that the serum-free culture medium has good industrial application prospect.

The present invention belongs to the field of biotechnology, and discloses a serum-free culture medium with specific chemical compositions for in vitro culture and amplification of bone marrow mesenchymal stem cells. By adding insulin, transferrin, ethanolamine, sodium selenite, growth factors, adherent factors, hormone, putrescine, inorganic salt, vitamin, albumin and antioxidant into a basic culture medium, the bone marrow mesenchymal stem cells can attach to the culture medium under a serum free condition, so the in vitro culture and amplification are realized, the potential of multi-directional differentiation is maintained, and the amplified cells can be induced to be osteoblast and lipocyte in vitro. The serum-free culture medium has the advantages that the clinic level cell products for human produced by the serum free culture medium can effectively avoid the potential risk of producing cell products by serum culture medium. The drawing appended is a photo of the confluence of the bone marrow mesenchymal stem cells cultured by the serum-free culture medium.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

The present invention provides methods and compositions for modulating polyamine pathway activity as a means for ameliorating neurodegenarative disorders. In particular, for ameliorating the symptoms or onset of amyotrophic lateral sclerosis (ALS) by modulating the gene and protein products involved the polyamine pathway, such as by inhibiting the enzyme, ornithine decarboxylase (ODC), involved in the synthesis of the polyamine, putrescine. Compositions and methods are disclosed for inhibiting the polyamine pathway producing lower polyamine levels resulting in a beneficial effect on ALS. This can be accomplished by using modulating agents such as analogs, or polyamine analogs, and antiproliferative drugs. Screening assays for pharmacological agents that are capable of decreasing polyamine levels and / or reducing cell proliferation are also disclosed.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

Compositions and methods for isolating and expanding human mesenchymal stem / progenitor cells through multiple passages in defined serum-free environments are provided. The culture media compositions includes a basal medium supplemented with a nutrient mixture such as Ham's F12 nutrient mixture, glutamine, buffer solutions such as sodium bicarbonate and hepes, serum albumin, a lipid mixture, insulin, transferrin, putrescine, progesterone, fetuin, hydrocortisone, ascorbic acid or its analogues such as ascorbic acid-2-phosphate, fibroblast growth factor and transforming growth factor β, and are free of serum or other undefined serum substitutes such as platelet lysate. Methods employing these compositions and protein-coated surfaces for the isolation of mesenchymal stem / progenitor cells from human bone marrow and other tissues such as adipose tissue are also provided. Finally, methods are also provided for serially expanding these cells through multiple passages without losing mesenchymal stem cell-specific proliferative, phenotypical and differentiation characteristics.

The invention relates to an SFM (serum-free medium) for culturing MSCs (mesenchymal stem cells). Based on volume, the SFM comprises the following components: 10.2 grams per liter of alpha-MEM (alpha-minimum essential medium), 2.4 grams per liter of sodium bicarbonate, 1 to 5 millimoles of L-glutamine, 50 to 300 milligrams per liter of poloxamer 188, 2 to 8 grams per liter of recombinant human albumin, 10 to 20 milligrams per liter of recombinant human transferrin, 2 to 10 milligrams per liter of recombinant human insulin, 1 to 5 millimoles per liter of Hepes, 50 nanomoles of beta-mercaptoethanol, 0.1 to 1 milligram per liter of lipid, 1 to 5 milligrams per liter of trace element, 0.1 to 5 milligrams per liter of glutathione, 0.5 to 5 milligrams per liter of para-aminobenzoic acid, 1 to 50 nanograms per milliliter of hydrocortisone, 20 to 50 milligrams per liter of vitamin PP, 5 to 50 milligrams per liter of vitamin C, 2 to 10mu M of compound shown in a formula I, 5 to 20mu M of compound shown in a formula II, 10 to 20 nanograms per milliliter of progestin, 1 to 10 milligrams per liter of putrescine, 1 to 10 international units per liter of heparin, 1 to 10 nanograms per milliliter of EGF (epidermal growth factor), 1 to 10 nanograms per milliliter of b-FGF (b-fibroblast growth factor), 1 to 10 nanograms per milliliter of HGF (hepatocyte growth factor) and 1 to 10 nanograms per milliliter of VEGF (vascular endothelial growth factor). The SFM for culturing the MSCs is a BPS-SFM which has determinate chemical components and is free of animal-derived substances.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

The present invention relates to a mutant microorganism having a high ability of producing putrescine in which genes associated with the decomposition or use pathway of putrescine are weakened or deleted, in a microorganism having a putrescine production metabolic pathway, and to a method for preparing putrescine with a high yield rate by culturing the mutant microorganism under an anaerobic condition. The mutant microorganism having a high ability of producing putrescine according to the present invention is useful for the high-yield production of putrescine and has broad industrial application.

The present invention relates to a preparation method of 4-[3-(2-butyl-amino) propoxy]- benzoic alkyl ester (a compound of formula (1)). The p-hydroxide benzene methyl formate and 1, 3 methylparaben react to prepare compound III under alkaline conditions. Then the products and putrescine dock to synthesize compound I under alkaline conditions. The compound is a key intermediate for preparing drugs of treating arrhythmic diseases, hydrochloride Juenaidalong. Wherein, R represents C1-C4 alkyl; X, X1 and X2 are halogen atoms, such as Cl, Br and I; but X1 and X2 are different from each other.

Nutrient compositions and methods that sustain and promote positive metabolic energy levels in a targeted manner are disclosed. Methods utilize endogenous energy stores (fat oxidation), increase use of those stores (increasing transport rate), increase available energy (increasing the ability to perform ADP to ATP phosphorylation,) as well as decrease catabolism and increase protein synthesis. Compositions are also disclosed, and include Mono- or Dicreatine-HMB salt; Putrescine Dihydrochloride; Alanine; L-Glutamine, which may be combined with Alanine in a 1:2 to 2:1 molecular ratio; Trimethylglycine; and Guanidinopropionic Acid.

The invention discloses a cold-resistant agent for growth of Chinese trichosanthes. The cold-resistant agent comprises the following components by weight: 5-7.5mg of abscisic acid, 0.03-0.06g of salicylic acid, 0.6-1g of calcium nitrate, 0.035-0.04g of putrescine, 0.05g of spermidine, 0.45g of urea, 0.3g of monopotassium phosphate, 0.04g of citric acid, 0.1g of malic acid, 0.05g of chitosan, and 1L of water. The invention simultaneously discloses a using method of the cold-resistant agent for growth of Chinese trichosanthes. The method comprises the following steps of: spraying leaves of a Chinese trichosanthes plant by a Chinese trichosanthes cold-resistant agent until the leaves drip 12-48 hours before a cold wave comes. By adopting the cold-resistant agent for growth of the Chinese trichosanthes disclosed by the invention, the ability of the Chinese trichosanthes for resisting low-temperature damage can be obviously enhanced; and the stability of a Chinese trichosanthes cell membrane also can be enhanced.

The invention discloses a blood serum-free culture medium suitable for the suspension culture of CHO cells with large scale and high density. The culture medium takes DMEM / F12 (v / v, 1:1) as a base culture medium, and adds the other substances such as insulin, putrescine, transferrin and microelement, etc. The blood serum-free culture medium has the advantages that: the culture medium can support the CHO cells to fast growth under the condition of suspension culture; the protein content is very low, and the chemical composition is basically clear; the cells are grown one by one by means of suspension, so that the living cell density and the cell living rate are higher than those of the serum culture and the similar commercialized serum-free culture; and the cost is lower.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

The invention discloses a lactobacillus plantarum and its application in the high-acid yellow wine production for acid modulation, belonging to the field of rice wine brewing technology. The lactobacillus plantarum CGMCC No.12757, isolated from rice wine fermented mash. The lactobacillus plantarum comprises of total acid of rice wine produced of more than 17.5g / L (in lactic acid), lactic acid content of 70% or more accounting for the total organic acid, 14% (v / v) or more of alcohol content, 52mg / L biogenic amine content (the total amount of putrescine, histamine and tyramine), far lower than the rancid rice wine in the storage process, which can produce high-acid rice wine for acid modulation, greatly facilitating the production of low-grade rice wine.

The invention provides a serum-free human amniotic mesenchymal stem cell culture medium, and belongs to the technical field of stem cells. The serum-free human amniotic mesenchymal stem cell culture medium comprises the following components: an IMDM basal culture medium, recombinant human insulin, recombinant transferrin, vitamin C, human serum albumin, fibroblast growth factors, platelet-derived growth factors, epidermal growth factors, insulin-like growth factors, transforming growth factors, fibronectin, fetuin, putrescine salt and recombinant human activin. The serum-free human amniotic mesenchymal stem cell culture medium provided by the invention does not need a culture bottle coating process, and is good in cell adherence and morphology and high in cell proliferation rate.

The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.

Belonging to the field of biotechnology and fermentation, the invention discloses a method for degradation of biogenic amines in wine making by lactobacillus plantarum. The strain is obtained by screening the surfaces of wine grapes, not only can effectively reduce the content of biogenic amines in an MRS medium added with putrescine, histamine and tyramine, but also can be directly applied in the wine making process. With good high acid and high alcohol content and high SO2 tolerance, and very high malic acid-lactic acid conversion rate, the strain can effectively decrease the content of biogenic amines in the wine making process.

The invention discloses a clinical-grade serum-free medium for adherent culture of human neural stem cells. The medium disclosed by the invention comprises a basic medium, basic nutrition additives, plant-based human serum albumins, saccharides, lipid, hormones, antioxidants and related substances for promoting metabolism; the basic medium is prepared from commercial DMEM / F12 and a commercial neurobasal medium according to a ratio of 1:1; the basic nutrition additives comprise insulin, holo-transferrin, apo-transferrin, putrescine, progesterone and sodium selenite; the hormones comprise biotin, corticosterone, lipoic acid, Ve and Ve acetic ester; the antioxidants comprise human-derived catalase, human-derived superoxide dismutase, glutathione and Vc; the related substances for promoting metabolism comprise carnitine, T3 and ethanol amine. The medium can improve a cell expansion speed by two to three times, well keeps stem properties of the neural stem cells, keeps the cell differentiation potential of the neural stem cells and eliminates potential animal-origin endotoxin and viral pollution.

The invention discloses dendritic flame-retardant layered silicate and a preparation method thereof, and the preparation method comprises the following steps: (1) mixing inorganic montmorillonite and high heat-resistant organic intercalator, adding ethanol water solution, stirring for 2-4h at 75-80DEG C and collecting high heat-resistant organic montmorillonite; (2) stirring the high heat-resistant organic montmorillonite, putrescine, acrylonitrile and catalyst for 5-8h at 70-90DEG C, and then collecting dendritic organic montmorillonite from the product; and (3) stirring the dendritic organic montmorillonite and alpha-(diphenylphosphino) acetic acid for 1-2h at 60-80DEG C, then adding zinc borate, and stirring for 2-4h at 70-90DEG C, thus obtaining the dendritic flame-retardant layered silicate. The dendritic flame-retardant layered silicate is applicable to rubber industry and cable industry, which can not only replace the traditional environmental-unfriendly flame-retardant and reinforcing material of high price, but also improve the performance of rubber material, such as flame resistance, mechanical properties and the like.

The invention provides a method for synthesizing chlorinated formic acid 9-fluoren methyl, which comprises the following steps: (1) 9-fluoren methyl and triphosgene are reacted for 0.5 to 3 hours in ice bath, under the existence of catalyst and in organic solvent; the catalyst is chosen from N with nucleophilicity, N-dimethylformamide, acetone, diisopropyl ethyl amine, diethylamine, triethylamine, ethyleendiamine, putrescine, imidazole, and the solvent is chosen from benzene, toluene, cyclohexane, hexane, heptane, cyclopentane, dichloromethane, chloroform and the compound thereof; (2) the temperature raises to 20 to 50 DEG C, and the mixture reacts for 1 to 5 hours. The method for synthesizing chlorinated formic acid 9-fluoren methyl adopts safe solid phosgene as reactant, and is simple in technique, convenient in safety, mild in condition, high in yield and easy in realization of industrialization production; the solvent can be used repeatedly after being processed simply, and the products do not need to be refined, and purity is up to more than 98 percent.

The invention discloses a Chinese hamster ovary culture medium as well as a preparation method and application thereof. The culture medium comprises ascorbic acid, pyridoxine hydrochloride, vitamin B12, nicotinamide, riboflavin, thiamine chloride, choline chloride, folic acid, calcium pantothenate, sodium pyruvate, reduced glutathione, inositol, biotin, hypoxanthine, putrescine, lipoic acid, linoleic acid, thymine, glucose, HEPES (2-hydroxyethyl) buffer solution, dextran sulphate, Pluronic-F68, various amino acids and salts thereof and various inorganic salts. The culture medium has low cost, and the component does not contain animal origin ingredients, does not contain protein and has definite chemical ingredients. All ingredients are cooperated and blended, and the Chinese hamster ovary culture medium can satisfy the basic growth requirement of cells, and can effectively regulate, transfer and control the culture of cells so as to realize good high-density cell culture. Compared with the culture effect of the existing culture medium, the culture effect of culture medium disclosed in the invention is at least equivalent to even superior to the existing culture medium.

The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.

The invention discloses an efficient liquid-phase chromatographic detection method for content of polyamines (putrescine, spermidine and spermine) in a goose tissue. Chromatographic conditions of the method are as follows: a C18 chromatographic column, 5 mu m, 4.6*250mm; a volume ratio of mobile phase methanol: water of 62:38; flow velocity of 1.0 mL / minute; ultraviolet detection wavelength of 229 nm; and a column temperature of 25 DEG C. A tissue solid-phase extracting method comprises the following steps: adding 0.1g of tissue into 1 mL of 5% HClO4 and 10 mu L of an internal standard substance for homogenizing and extracting supernatant; adding 2 mL of 2.5 mol / mL NaOH and 1.4 mu L of benzoyl chloride, carrying out water-bath for 30 minutes at a temperature of 40 DEG C; adjusting pH value to 7.0, then, adding into the C18 column, and flushing by 15 mL of water and 15 mL of 15% methanol; and eluting by 0.5 mu L of methanol. The scheme can effectively separate putrescine, spermidine and spermine in the tissue, and can quickly and accurately detect content of the polyamines in the goose tissue, is simple and convenient to operate, high in accuracy and good in repeatability.

The invention provides a serum-free, animal-derived-component-free culture medium with definite components and application thereof, and relates to the technical field of cell culture. The serum-free and animal-derived-component-free culture medium with definite components comprises a basic culture medium and cytokines, hormones, transferrin, albumin, fetal globulin, putrescine and serotonin whichare added into the basic culture medium. According to the serum-free, animal-derived-component-free culture medium with definite components, substances such as cytokines, hormones and the like are added into a basic culture medium to replace serum, so that the culture of human cells is realized. The components of the culture medium are definite, no serum and animal-derived-component components areadded, so that the technical problems of poor repeatability of the traditional serum-containing culture medium for cell culture, low cell activity and low cell amplification efficiency of other serum-free culture mediums can be mitigated.

The invention discloses an improved Neurobasal B27 culture medium. One liter of a Neurobasal culture solution comprises 0.09-0.11 g of biotin, 18-22 micrograms of corticosterone, 1-3 g of L-carnitine, 0.9-1.1 g of linoleic acid, 0.9-1.1 g of ethanol amine, 0.9-1.1 g of linolenic acid, 13-17 g of D(+)-galactose, 6-7 micrograms of progesterone, 15-17 g of putrescine dihydrochloride, 0.08-0.12 g of retinyl acetate, 2.2-2.8 g of thyroid-hormone-removing bovine serum albumin, 0.8-1.5 g of DL-alpha-tocopherol, 2-3 g of catalase, 0.9-1.2 g of DL-alpha-tocopheryl acetate, 0.8-1.3 g of reduced glutathione, 42-52 micrograms of lipoic acid, 2-3 g of superoxide dismutase, 4-6 g of transferring, 3-5 g of human insulin and 0.12-0.16 mg of sodium selenite. The invention further discloses a preparing method and application of the improved Neurobasal B27 culture medium. The improved Neurobasal B27 culture medium can be used for researching the influence of thyroid hormones on the process that embryonic stem cells are differentiated into nerve cells.

The invention particularly discloses an insect attractant capable of improving the pollination rate and the fruit set percentage of a jujube tree, and application of the insect attractant. The insectattractant comprises a nutrient solution and an animal and vegetable decomposition liquid, wherein the nutrient solution comprises the following ingredients by weight percent: 27.56-38.38% of honey, 0.01-0.09% of putrescine, 0.01-0.09% of spermidine, 0.1-1.3% of salicylic acid, 0.1-0.5% of gibberellin and 60.36-71.5% of urea; the animal and vegetable decomposition liquid is a supernate obtained byfiltering after fermentation of egg, cooked beans and chicken entrails for 7-8 days at 25-35 DEG C; and the nutrient solution, the animal and vegetable decomposition liquid and water are compounded at the proportion of 3:1:16. By spraying the insect attractant onto jujube flowers in the flowering stage, the proportion of fly flower-visiting insects in the total flower-visiting insect number is averagely increased by 5-8 percent. The insect attractant can be sprayed to achieve a remarkable trapping effect on flies and bees, the number and the flower-visiting frequency of flower-visiting insects are increased in a unit time, and the pollination and fertilization rates of jujube flowers are increased. After spraying of 100 times and 200 times of the insect attractant, the single-plant yieldsof the jujube tree are increased by 56.94% and 35.08% respectively, the prune single-plant yields are increased b6 36.36% and 25.38% respectively, and the almond single-plant yields are increased by48.11% and 44,30% respectively.

The invention relates to the field of cell culture medium, and in particular to a non-serum non-animal-origin-additive insect cell culture medium. The medium comprises mainly the following components: basic culture medium, glucose, inorganic salt, vitamins, L-arginine, L-agedoite, L-glycocoll, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine, L-proline, L-glutamine, yeast extracts, recombulin, malic acid, allomaleic acid, cholesterol, linoleic acid, granulesten, putrescine, glutathione, glycerol and fructosan. The culture medium can promote the growth of insect cell and is suitable for the large scale breeding of insect cell and the expression of recombination protein.